Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006861

Physical Location & Seq

Genomic Coordinates Reverse (-)

6021379 .. 6027299

Gene Structure 5921 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G18230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGGTGCGAGCCGGAAATTACAAGGCGAGATAGATCGGGTGCTGAAGAAGGTTCAAGAAGGTGTTGATGTTTTCGACAGCATCTGGAACAAGGTATATGATACAGACAATGTTAATCAAAAGGAAAAGTTTGAGGCGGACTTGAAGAAGGAAATCAAGAAGCTGCAGCGGTATAGAGACCAGATCAAGACATGGATTCAGTCTAGTGAGATCAAAGATAAGAAAGTCAGTGCATCTTATGAGCAATCCCTGGTGGATGCTCGGAAGCTTATTGAGAAAGAGATGGAGAGGTTTAAGATATGTGAAAAAGAGACCAAGACAAAAGCCTTCTCCAAGGAAGGACTGGGTCAGCAACCTAAAACTGACCCAAAAGAGAAAGCAAAGTCAGAGACAAGGGATTGGTTGAACAATGTGGTGAGTGAACTGGAGTCGCAGATTGATAGCTTTGAAGCTGAGTTGGAAGGACTGTCTGTCAAAAAAGGAAAGACAAGACCGCCCAGATTGACTCATCTTGAGACATCTATTACAAGACACAAGGATCACATAATAAAGTTGGAACTGATCTTGAGGCTTCTGGACAATGATGAATTAAGTCCAGAACAAGTAAATGACGTCAAAGATTTTCTGGATGATTATGTTGAACGAAATCAGGATGATTTTGATGAATTCAGTGATGTCGATGAGCTCTATAGCACGTTGCCACTAGATGAGGTGGAGGGTCTTGAAGATCTAGTTACCGCTGGCCCACTTGTCAAGGGTACTCCTTTAAGCATGAAGAGTTCTTTGGCAGCGTCAGCATCTCAAGTTCGGAGCATAAGTTTGCCAACTCACCATCAAGAGAAAACAGAGGATACATCTTTACCGGATAGCAGTGCTGAGATGGTTCCAAAAACCCCTCCGCCAAAGAATGGTGCAGGCCTTCACTCAGCACCATCAACACCTGCCGGAGGACGTCCAAGTTTGAACGTGCCTGCCGGTAATGTTTCAAATACATCAGTTACCTTATCAACTTCTATTCCTACTCAAACTTCCATAGAAAGCATGGGGAGTTTGTCTCCCGTGGCTGCCAAGGAAGAAGACGCAACAACCTTGCCTTCTCGTAAACCACCCTCATCTGTTGCGGATACTCCATTGAGGGGCATTGGTAGAGTTGGTATCCCCAACCAACCCCAACCAAGCCAGCCTCCGTCTCCTATTCCAGCTAACGGGTCTCGCATTAGTGCAACTTCAGCTGCTGAAGTTGCAAAGAGAAATATAATGGGAGTTGAGAGCAACGTCCAACCTCTTACTTCTCCACTGAGCAAAATGGTGTTGCCACCAACTGCAAAGGGTAATGATGGAACTGCCTCTGATAGCAACCCTGGTGATGTTGCGGCTAGTATTGGTAGAGCTTTTTCACCATCTATTGTATCTGGTTCGCAGTGGAGGCCTGGTAGTCCCTTTCAGAGTCAGAATGAAACGGTTCGTGGGAGAACTGAAATAGCACCAGACCAAAGAGAGAAATTCTTACAGAGATTACAGCAAGTACAGCAAGGCCATGGTAACCTCTTAGGCATACCTTCTTTATCTGGAGGAAACGAGAAGCAGTTTTCTTCACAACAGCAAAATCCTCTTTTACAGCAGAGCTCTTCCATCTCTCCTCATGGAAGCTTGGGAATCGGAGTTCAGGCACCAGGTTTTAATGTCATGAGTTCTGCCTCCTTACAGCAGCAATCAAATGCCATGAGTCAACAATTGGGTCAGCAACCTTCTGTTGCAGATGTAGACCATGTCAGAAATGATGATCAATCCCAGCAAAACTTACCTGATGATTCAGCTTCCATAGCAGCTTCAAAAGCTATTCAAAGTGAGGATGACTCTAAAGTTCTATTTGATACTCCGTCGGGAATGCCCAGCTACATGTTGGATCCAGTACAAGTATCTAGCGGTCCTGATTTCTCTCCTGGACAACCTATACAACCGGGTCAATCTTCAAGTAGCCTCGGGGTCATTGGACGGAGAAGTAACTCTGAGTTAGGAGCCATTGGTGACCCTTCAGCTGTAGGGCCAATGCATGATCAAATGCACAATCTCCAGATGCTTGAAGCTGCTTTTTACAAACGTCCTCAACCCTCAGATTCAGAACGTCCTAGACCCTATTCTCCGAGGAACCCAGCAATCACACCTCAAACATTTCCCCAAACACAAGCACCAATCATAAACAACCCTTTGCTCTGGGAACGGTTAGGCAGCGATGCTTATGGAACCGATACTTTGTTCTTTGCGTTCTACTATCAGCAGAACTCATACCAGCAATATCTTGCTGCAAAAGAGCTGAAGAAACAGTCATGGAGATACCACAGGAAGTTCAACACTTGGTTTCAGAGACATAAAGAGCCAAAGATTGCAACCGATGAATATGAACAAGGAGCCTACGTTTACTTCGATTTCCAAACCCCGAAAGACGAGAATCAAGAAGGAGGATGGTGCCAAAGGATCAAAAACGAGTTCACATTTGAATACAGTTATCTTGAAGATGAACTCGTCGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

NOT2_3_5 Not3

Protein Analysis

843

Amino Acids

92.95

Weight (kDa)

5.33

Isoelectric Point (pI)

55.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Not3	PF04065	4 - 235	1.3e-82	Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C	PF04153	699 - 837	8.3e-32	NOT2/NOT3/NOT5 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006861)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18230 AT5G18230 AT5G18230 AT5G18230
fragaria_vesca	FvH4_5g18220 FvH4_5g18220 FvH4_5g18220 FvH4_5g18220
malus_domestica	MD06G1237800.v1.1 MD14G1244600.v1.1
prunus_persica	Prupe.5G242100_v2.0.a1 Prupe.5G242100_v2.0.a1 Prupe.5G242100_v2.0.a1
pyrus_communis	pycom06g21350 pycom14g20570
rosa_chinensis	RchiOBHm_Chr7g0177371
rosa_laevigata	RLG00000005542
rosa_multiflora	Rmu_sc0005310.1_g000004
rosa_roxburghii	Rroxscaffold_3G00275910
rosa_rugosa	Rorug06G0405300 Rorug06G0405400
rosa_samantha	Rh7AG005000 Rh7BG005000 Rh7CG005100 Rh7DG004800
rosa_wichuraiana	Rw7G000410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	949
AatII	GACGTC	2 cut(s)	615, 955
Acc36I	ACCTGC	1 cut(s)	949
AccB1I	GGYRCC	2 cut(s)	1669, 2466
AccB7I	CCANNNNNTGG	1 cut(s)	908
AccI	GTMKAC	1 cut(s)	1764
AciI	CCGC	8 cut(s)	137, 169, 494, 738, 899, 1121, 1373, 1926
AclWI	GGATC	4 cut(s)	546, 1901, 1914, 2483
AcsI	RAATTY	2 cut(s)	665, 1502
AcuI	CTGAAG	5 cut(s)	65, 1212, 1257, 2019, 2336
AcyI	GRCGYC	2 cut(s)	612, 952
AfaI	GTAC	3 cut(s)	760, 1527, 1914
AfiI	CCNNNNNNNGG	4 cut(s)	908, 947, 1136, 1205
AflIII	ACRYGT	1 cut(s)	1899
AhdI	GACNNNNNGTC	1 cut(s)	470
AjnI	CCWGG	5 cut(s)	249, 1360, 1429, 1672, 1942
AjuI	GAANNNNNNNTTGG	2 cut(s)	767, 799
AloI	GAACNNNNNNTCC	2 cut(s)	1647, 1679
Alw21I	GWGCWC	2 cut(s)	687, 1628
Alw26I	GTCTC	8 cut(s)	171, 305, 383, 509, 1059, 1194, 1215, 2359
AlwI	GGATC	4 cut(s)	546, 1901, 1914, 2483
AlwNI	CAGNNNCTG	2 cut(s)	84, 1235
Ama87I	CYCGRG	1 cut(s)	1982
AoxI	GGCC	5 cut(s)	742, 916, 1427, 1534, 2045
ApoI	RAATTY	2 cut(s)	665, 1502
Asp700I	GAANNNNTTC	1 cut(s)	1461
AspS9I	GGNCC	3 cut(s)	743, 1928, 2045
AsuC2I	CCSGG	1 cut(s)	1962
AsuHPI	GGTGA	5 cut(s)	427, 821, 1376, 1389, 2039
AvaI	CYCGRG	1 cut(s)	1982
AvaII	GGWCC	1 cut(s)	1928
BamHI	GGATCC	1 cut(s)	1906
BanI	GGYRCC	2 cut(s)	1669, 2466
BanII	GRGCYC	2 cut(s)	687, 1628
BbsI	GAAGAC	1 cut(s)	1083
Bbv12I	GWGCWC	2 cut(s)	687, 1628
BccI	CCATC	8 cut(s)	277, 840, 874, 940, 1331, 1408, 1640, 2457
BciT130I	CCWGG	5 cut(s)	251, 1362, 1431, 1674, 1944
BciVI	GTATCC	3 cut(s)	844, 1117, 1166
BclI	TGATCA	2 cut(s)	1783, 2056
BcnI	CCSGG	1 cut(s)	1962
BcoDI	GTCTC	8 cut(s)	171, 305, 383, 509, 1059, 1194, 1215, 2359
BfaI	CTAG	6 cut(s)	204, 704, 731, 1377, 1923, 2130
BfmI	CTRYAG	3 cut(s)	164, 688, 2040
BfuAI	ACCTGC	1 cut(s)	949
BfuI	GTATCC	3 cut(s)	844, 1117, 1166
BglII	AGATCT	1 cut(s)	727
Bme1390I	CCNGG	6 cut(s)	251, 1362, 1431, 1674, 1944, 1962
Bme18I	GGWCC	1 cut(s)	1928
BmeRI	GACNNNNNGTC	1 cut(s)	470
BmeT110I	CYCGRG	1 cut(s)	1982
BmgT120I	GGNCC	3 cut(s)	743, 1928, 2045
BmiI	GGNNCC	8 cut(s)	885, 1671, 1908, 2020, 2150, 2245, 2410, 2468
BmrFI	CCNGG	6 cut(s)	251, 1362, 1431, 1674, 1944, 1962
BmrI	ACTGGG	1 cut(s)	353
BmsI	GCATC	6 cut(s)	90, 242, 247, 806, 2067, 2224
BmuI	ACTGGG	1 cut(s)	353
BpiI	GAAGAC	1 cut(s)	1083
BplI	GAGNNNNNCTC	4 cut(s)	1039, 1071, 1841, 1873
BpmI	CTGGAG	3 cut(s)	446, 1590, 2057
BpuEI	CTTGAG	3 cut(s)	533, 586, 786
BpuMI	CCSGG	1 cut(s)	1962
BsaHI	GRCGYC	2 cut(s)	612, 952
BsaI	GGTCTC	3 cut(s)	171, 305, 1215
BsaJI	CCNNGG	8 cut(s)	249, 333, 1059, 1068, 1360, 1537, 1981, 2144
BsaWI	WCCGGW	1 cut(s)	862
BsaXI	ACNNNNNCTCC	6 cut(s)	802, 832, 1417, 1447, 2400, 2430
Bsc4I	CCNNNNNNNGG	4 cut(s)	908, 947, 1136, 1205
Bse118I	RCCGGY	1 cut(s)	974
Bse1I	ACTGG	3 cut(s)	348, 429, 1910
BseBI	CCWGG	5 cut(s)	251, 1362, 1431, 1674, 1944
BseDI	CCNNGG	8 cut(s)	249, 333, 1059, 1068, 1360, 1537, 1981, 2144
BseGI	GGATG	5 cut(s)	262, 634, 658, 1858, 2468
BseLI	CCNNNNNNNGG	4 cut(s)	908, 947, 1136, 1205
BseMII	CTCAG	6 cut(s)	444, 867, 939, 1289, 2001, 2127
BseNI	ACTGG	3 cut(s)	348, 429, 1910
BseRI	GAGGAG	1 cut(s)	1629
BseYI	CCCAGC	3 cut(s)	1791, 1892, 2152
BsgI	GTGCAG	1 cut(s)	933
BshFI	GGCC	5 cut(s)	744, 918, 1429, 1536, 2047
BshNI	GGYRCC	2 cut(s)	1669, 2466
BsiHKAI	GWGCWC	2 cut(s)	687, 1628
BsiHKCI	CYCGRG	1 cut(s)	1982
BsiSI	CCGG	5 cut(s)	13, 863, 945, 975, 1961
BslFI	GGGAC	1 cut(s)	1422
BslI	CCNNNNNNNGG	4 cut(s)	908, 947, 1136, 1205
BsmAI	GTCTC	8 cut(s)	171, 305, 383, 509, 1059, 1194, 1215, 2359
BsmBI	CGTCTC	1 cut(s)	1194
BsmFI	GGGAC	1 cut(s)	1422
BsmI	GAATGC	1 cut(s)	1893
BsnI	GGCC	5 cut(s)	744, 918, 1429, 1536, 2047
Bso31I	GGTCTC	3 cut(s)	171, 305, 1215
BsoBI	CYCGRG	1 cut(s)	1982
Bsp1286I	GDGCHC	2 cut(s)	687, 1628
Bsp19I	CCATGG	1 cut(s)	1537
BspACI	CCGC	8 cut(s)	137, 169, 494, 738, 899, 1121, 1373, 1926
BspANI	GGCC	5 cut(s)	744, 918, 1429, 1536, 2047
BspCNI	CTCAG	6 cut(s)	445, 868, 938, 1290, 2002, 2126
BspHI	TCATGA	1 cut(s)	1686
BspLI	GGNNCC	8 cut(s)	885, 1671, 1908, 2020, 2150, 2245, 2410, 2468
BspMAI	CTGCAG	1 cut(s)	168
BspMI	ACCTGC	1 cut(s)	949
BspPI	GGATC	4 cut(s)	546, 1901, 1914, 2483
BspQI	GCTCTTC	1 cut(s)	1633
BspT107I	GGYRCC	2 cut(s)	1669, 2466
BspTNI	GGTCTC	3 cut(s)	171, 305, 1215
BsrFI	RCCGGY	1 cut(s)	974
BsrI	ACTGG	3 cut(s)	348, 429, 1910
BssAI	RCCGGY	1 cut(s)	974
BssECI	CCNNGG	8 cut(s)	249, 333, 1059, 1068, 1360, 1537, 1981, 2144
BssNI	GRCGYC	2 cut(s)	612, 952
BssT1I	CCWWGG	3 cut(s)	333, 1068, 1537
Bst2UI	CCWGG	5 cut(s)	251, 1362, 1431, 1674, 1944
Bst4CI	ACNGT	5 cut(s)	468, 1462, 2223, 2325, 2504
Bst6I	CTCTTC	2 cut(s)	770, 1633
BstACI	GRCGYC	2 cut(s)	612, 952
BstC8I	GCNNGC	5 cut(s)	10, 742, 916, 972, 1181
BstDEI	CTNAG	7 cut(s)	453, 876, 925, 1298, 1549, 2010, 2113
BstDSI	CCRYGG	2 cut(s)	1059, 1537
BstEII	GGTNACC	2 cut(s)	1541, 2027
BstENI	CCTNNNNNAGG	1 cut(s)	945
BstF5I	GGATG	5 cut(s)	262, 634, 658, 1858, 2468
BstMAI	GTCTC	8 cut(s)	171, 305, 383, 509, 1059, 1194, 1215, 2359
BstMWI	GCNNNNNNNGC	7 cut(s)	794, 1229, 1241, 1426, 1528, 1823, 1835
BstNI	CCWGG	5 cut(s)	251, 1362, 1431, 1674, 1944
BstNSI	RCATGY	1 cut(s)	1903
BstPI	GGTNACC	2 cut(s)	1541, 2027
BstSCI	CCNGG	6 cut(s)	249, 1360, 1429, 1672, 1942, 1960
BstSFI	CTRYAG	3 cut(s)	164, 688, 2040
BstV2I	GAAGAC	1 cut(s)	1083
BstX2I	RGATCY	2 cut(s)	727, 1906
BstYI	RGATCY	2 cut(s)	727, 1906
BsuI	GTATCC	3 cut(s)	844, 1117, 1166
BsuRI	GGCC	5 cut(s)	744, 918, 1429, 1536, 2047
BtgI	CCRYGG	2 cut(s)	1059, 1537
BtgZI	GCGATG	1 cut(s)	2247
BtsCI	GGATG	5 cut(s)	262, 634, 658, 1858, 2468
BtsI	GCAGTG	2 cut(s)	877, 1427
BtsIMutI	CAGTG	5 cut(s)	235, 676, 877, 1295, 1427
BveI	ACCTGC	1 cut(s)	949
Cac8I	GCNNGC	5 cut(s)	10, 742, 916, 972, 1181
CaiI	CAGNNNCTG	2 cut(s)	84, 1235
CciI	TCATGA	1 cut(s)	1686
Cfr10I	RCCGGY	1 cut(s)	974
Cfr13I	GGNCC	3 cut(s)	743, 1928, 2045
CseI	GACGC	2 cut(s)	780, 1088
CsiI	ACCWGGT	1 cut(s)	1672
Csp6I	GTAC	3 cut(s)	759, 1526, 1913
CviQI	GTAC	3 cut(s)	759, 1526, 1913
DdeI	CTNAG	7 cut(s)	453, 876, 925, 1298, 1549, 2010, 2113
DriI	GACNNNNNGTC	1 cut(s)	470
Eam1104I	CTCTTC	2 cut(s)	770, 1633
Eam1105I	GACNNNNNGTC	1 cut(s)	470
EarI	CTCTTC	2 cut(s)	770, 1633
EciI	GGCGGA	2 cut(s)	152, 888
Ecl136II	GAGCTC	2 cut(s)	685, 1626
Eco130I	CCWWGG	3 cut(s)	333, 1068, 1537
Eco147I	AGGCCT	2 cut(s)	918, 1429
Eco24I	GRGCYC	2 cut(s)	687, 1628
Eco31I	GGTCTC	3 cut(s)	171, 305, 1215
Eco47I	GGWCC	1 cut(s)	1928
Eco53kI	GAGCTC	2 cut(s)	685, 1626
Eco57I	CTGAAG	5 cut(s)	65, 1212, 1257, 2019, 2336
Eco88I	CYCGRG	1 cut(s)	1982
Eco91I	GGTNACC	2 cut(s)	1541, 2027
EcoICRI	GAGCTC	2 cut(s)	685, 1626
EcoNI	CCTNNNNNAGG	1 cut(s)	945
EcoO65I	GGTNACC	2 cut(s)	1541, 2027
EcoRI	GAATTC	1 cut(s)	665
EcoRII	CCWGG	5 cut(s)	249, 1360, 1429, 1672, 1942
EcoT14I	CCWWGG	3 cut(s)	333, 1068, 1537
EcoT22I	ATGCAT	1 cut(s)	2055
EcoT38I	GRGCYC	2 cut(s)	687, 1628
ErhI	CCWWGG	3 cut(s)	333, 1068, 1537
Esp3I	CGTCTC	1 cut(s)	1194
FaqI	GGGAC	1 cut(s)	1422
FbaI	TGATCA	2 cut(s)	1783, 2056
FblI	GTMKAC	1 cut(s)	1764
FokI	GGATG	5 cut(s)	269, 641, 665, 1865, 2475
FriOI	GRGCYC	2 cut(s)	687, 1628
FspBI	CTAG	6 cut(s)	204, 704, 731, 1377, 1923, 2130
GsaI	CCCAGC	3 cut(s)	1795, 1896, 2156
GsuI	CTGGAG	3 cut(s)	446, 1590, 2057
HaeIII	GGCC	5 cut(s)	744, 918, 1429, 1536, 2047
HapII	CCGG	5 cut(s)	13, 863, 945, 975, 1961
HgaI	GACGC	2 cut(s)	780, 1088
Hin1I	GRCGYC	2 cut(s)	612, 952
HincII	GTYRAC	1 cut(s)	1730
HindII	GTYRAC	1 cut(s)	1730
HindIII	AAGCTT	2 cut(s)	266, 1648
HpaII	CCGG	5 cut(s)	13, 863, 945, 975, 1961
HphI	GGTGA	5 cut(s)	427, 821, 1376, 1389, 2039
Hpy166II	GTNNAC	6 cut(s)	422, 1103, 1730, 1765, 2419, 2490
Hpy8I	GTNNAC	6 cut(s)	422, 1103, 1730, 1765, 2419, 2490
Hpy99I	CGWCG	2 cut(s)	1885, 2528
HpyCH4III	ACNGT	5 cut(s)	468, 1462, 2223, 2325, 2504
HpyCH4IV	ACGT	8 cut(s)	612, 695, 952, 966, 1275, 2101, 2125, 2415
HpyF10VI	GCNNNNNNNGC	7 cut(s)	794, 1229, 1241, 1426, 1528, 1823, 1835
HpyF3I	CTNAG	7 cut(s)	453, 876, 925, 1298, 1549, 2010, 2113
HpySE526I	ACGT	8 cut(s)	612, 695, 952, 966, 1275, 2101, 2125, 2415
Hsp92I	GRCGYC	2 cut(s)	612, 952
Ksp22I	TGATCA	2 cut(s)	1783, 2056
LguI	GCTCTTC	1 cut(s)	1633
LmnI	GCTCC	3 cut(s)	810, 2018, 2408
LweI	GCATC	6 cut(s)	90, 242, 247, 806, 2067, 2224
MabI	ACCWGGT	1 cut(s)	1672
MaeI	CTAG	6 cut(s)	204, 704, 731, 1377, 1923, 2130
MaeII	ACGT	8 cut(s)	612, 695, 952, 966, 1275, 2101, 2125, 2415
MaeIII	GTNAC	5 cut(s)	733, 997, 1541, 2003, 2027
MfeI	CAATTG	1 cut(s)	1733
MflI	RGATCY	2 cut(s)	727, 1906
MhlI	GDGCHC	2 cut(s)	687, 1628
MluCI	AATT	5 cut(s)	17, 587, 665, 1502, 1733
MlyI	GAGTC	5 cut(s)	437, 499, 1456, 1735, 1850
MmeI	TCCRAC	4 cut(s)	438, 534, 1303, 1884
Mph1103I	ATGCAT	1 cut(s)	2055
MroXI	GAANNNNTTC	1 cut(s)	1461
MseI	TTAA	5 cut(s)	114, 294, 590, 767, 1680
MspA1I	CMGCKG	4 cut(s)	169, 740, 1232, 2039
MspI	CCGG	5 cut(s)	13, 863, 945, 975, 1961
MspR9I	CCNGG	6 cut(s)	251, 1362, 1431, 1674, 1944, 1962
MunI	CAATTG	1 cut(s)	1733
Mva1269I	GAATGC	1 cut(s)	1893
MvaI	CCWGG	5 cut(s)	251, 1362, 1431, 1674, 1944
MwoI	GCNNNNNNNGC	7 cut(s)	794, 1229, 1241, 1426, 1528, 1823, 1835
NciI	CCSGG	1 cut(s)	1962
NcoI	CCATGG	1 cut(s)	1537
NlaIV	GGNNCC	8 cut(s)	885, 1671, 1908, 2020, 2150, 2245, 2410, 2468
NmuCI	GTSAC	1 cut(s)	2027
NsiI	ATGCAT	1 cut(s)	2055
NspI	RCATGY	1 cut(s)	1903
PagI	TCATGA	1 cut(s)	1686
PaqCI	CACCTGC	1 cut(s)	949
PceI	AGGCCT	2 cut(s)	918, 1429
PciI	ACATGT	1 cut(s)	1899
PciSI	GCTCTTC	1 cut(s)	1633
PcsI	WCGNNNNNNNCGW	1 cut(s)	2421
PctI	GAATGC	1 cut(s)	1893
PdmI	GAANNNNTTC	1 cut(s)	1461
PfeI	GAWTC	5 cut(s)	196, 1656, 1811, 2117, 2449
PflFI	GACNNNGTC	2 cut(s)	345, 1769
PflMI	CCANNNNNTGG	1 cut(s)	908
PfoI	TCCNGGA	1 cut(s)	1942
PleI	GAGTC	5 cut(s)	436, 499, 1455, 1734, 1850
PpsI	GAGTC	5 cut(s)	436, 499, 1455, 1734, 1850
PscI	ACATGT	1 cut(s)	1899
Psp124BI	GAGCTC	2 cut(s)	687, 1628
Psp6I	CCWGG	5 cut(s)	249, 1360, 1429, 1672, 1942
PspEI	GGTNACC	2 cut(s)	1541, 2027
PspFI	CCCAGC	3 cut(s)	1791, 1892, 2152
PspGI	CCWGG	5 cut(s)	249, 1360, 1429, 1672, 1942
PspN4I	GGNNCC	8 cut(s)	885, 1671, 1908, 2020, 2150, 2245, 2410, 2468
PspPI	GGNCC	3 cut(s)	743, 1928, 2045
PstI	CTGCAG	1 cut(s)	168
PstNI	CAGNNNCTG	2 cut(s)	84, 1235
PsuI	RGATCY	2 cut(s)	727, 1906
PsyI	GACNNNGTC	2 cut(s)	345, 1769
PvuII	CAGCTG	2 cut(s)	1232, 2039
RsaI	GTAC	3 cut(s)	760, 1527, 1914
RsaNI	GTAC	3 cut(s)	759, 1526, 1913
SacI	GAGCTC	2 cut(s)	687, 1628
SapI	GCTCTTC	1 cut(s)	1633
SaqAI	TTAA	5 cut(s)	114, 294, 590, 767, 1680
Sau96I	GGNCC	3 cut(s)	743, 1928, 2045
SchI	GAGTC	5 cut(s)	437, 499, 1456, 1735, 1850
ScrFI	CCNGG	6 cut(s)	251, 1362, 1431, 1674, 1944, 1962
SduI	GDGCHC	2 cut(s)	687, 1628
SexAI	ACCWGGT	1 cut(s)	1672
SfaNI	GCATC	6 cut(s)	90, 242, 247, 806, 2067, 2224
SfcI	CTRYAG	3 cut(s)	164, 688, 2040
SinI	GGWCC	1 cut(s)	1928
SmlI	CTYRAG	3 cut(s)	512, 565, 801
SmoI	CTYRAG	3 cut(s)	512, 565, 801
Sse9I	AATT	5 cut(s)	17, 587, 665, 1502, 1733
SseBI	AGGCCT	2 cut(s)	918, 1429
SsiI	CCGC	8 cut(s)	137, 169, 494, 738, 899, 1121, 1373, 1926
SspMI	CTAG	6 cut(s)	204, 704, 731, 1377, 1923, 2130
SstI	GAGCTC	2 cut(s)	687, 1628
StuI	AGGCCT	2 cut(s)	918, 1429
StyD4I	CCNGG	6 cut(s)	249, 1360, 1429, 1672, 1942, 1960
StyI	CCWWGG	3 cut(s)	333, 1068, 1537
TaaI	ACNGT	5 cut(s)	468, 1462, 2223, 2325, 2504
TaiI	ACGT	8 cut(s)	615, 698, 955, 969, 1278, 2104, 2128, 2418
TaqI	TCGA	3 cut(s)	75, 678, 2424
TasI	AATT	5 cut(s)	17, 587, 665, 1502, 1733
TatI	WGTACW	2 cut(s)	1525, 1912
TauI	GCSGC	1 cut(s)	1376
TfiI	GAWTC	5 cut(s)	196, 1656, 1811, 2117, 2449
Tru1I	TTAA	5 cut(s)	114, 294, 590, 767, 1680
Tru9I	TTAA	5 cut(s)	114, 294, 590, 767, 1680
TscAI	CASTG	5 cut(s)	235, 676, 877, 1302, 1427
TseFI	GTSAC	1 cut(s)	2027
Tsp45I	GTSAC	1 cut(s)	2027
TspDTI	ATGAA	7 cut(s)	600, 678, 788, 1470, 2409, 2415, 2532
TspGWI	ACGGA	3 cut(s)	1176, 1869, 2011
TspRI	CASTG	5 cut(s)	235, 676, 877, 1302, 1427
Tth111I	GACNNNGTC	2 cut(s)	345, 1769
Van91I	CCANNNNNTGG	1 cut(s)	908
VpaK11BI	GGWCC	1 cut(s)	1928
XagI	CCTNNNNNAGG	1 cut(s)	945
XapI	RAATTY	2 cut(s)	665, 1502
XceI	RCATGY	1 cut(s)	1903
XcmI	CCANNNNNNNNNTGG	3 cut(s)	340, 1039, 1900
XmiI	GTMKAC	1 cut(s)	1764
XmnI	GAANNNNTTC	1 cut(s)	1461
XspI	CTAG	6 cut(s)	204, 704, 731, 1377, 1923, 2130
ZraI	GACGTC	2 cut(s)	613, 953
Zsp2I	ATGCAT	1 cut(s)	2055

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.