Rw7G000410

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006861
Physical Location & Seq
Reverse (-)
342878 .. 349982
7105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000410.1

Sequence Viewer

Length: 2682 bp
ATGGGGGCGAGTCGGAAGCTCCAGGGCGAGATCGACCGGGTTCTCAAGAAGGTCCAAGAAGGCGTGGATGTGTTCGACAGCATTTGGAATAAGGTTTATGACACAGACAATGCGAACCAGAAGGAGAAGTTTGAGGCCGACTTGAAGAAGGAGATTAAGAAGCTCCAGCGGTACAGGGACCAAATCAAGACTTGGATTCAGTCCAGTGAAATCAAGGATAAGAAGGTGAGTGCCTCATACGAGCAGGCTCTGGTGGATGCTCGGAAGCTTATTGAGCGTGAAATGGAAAGGTTTAAGATATGCGAAAAGGAGACCAAAACCAAAGCTTTTTCTAAAGAGGGATTGGGCCAACAACCTAAAACTGATCCGAAGGAGAAGGCTAAATCAGAGACAAGGGATTGGATAAACACTGTGGTAGGGGAGTTGGAATCTCAGATTGACAGCTTTGAAGCTGAGATTGAGGGGGTATCAGTCAAGAAAGGAAAGGGCAGACCGCCTAGACTGACCCATCTAGAGACATCTATTACCCGGCATAAGGCTCATATAATGAAGTTGGAACTGATCTTAAGGTTATTGGATAATGATGAACTCAGTCCTGAGCAGGTCAATGATGTCAAAGACTTTTTGGAAGACTATGTTGAACGTAATCAGGAGGACTTTGATGAGTTTAGTGAAGTTGATGAGCTTTACAGTACCTTACCATTAGACAAGGTGGAGTCCCTTGAAGATATTGTTCCTCCTGGTCTTGTCAAGGGTGCACCGATGCTTGGCTTGAAGACTTCTTTGGCAGCATCAGCATCTCAAATGCCTCCCATTGTTATTACCACTAACCAACCAAGTACTCCTGTACAAGAGCCGGTTGAGGATACAGTTTCCCAGGATAACAGTAATCCTGATAACGTTGCTAGAACTCCACCTCCTAAAAGCAGTGCACTTGGTTCTGCTGCATCAACACCAACTGGGAATCATGCAACTCCTGTCTCTTTGAATGTTTCATCTCTCAGTTTGCCTGGTGTACCAACTGTTTCAGCTGTTGCTGGTTCAAATGCTGTTCGTGGTGTCACAGAGAATGCAGGGGCTGCTCGTTCTTCATCTCCTGTAAGTCTGTCTCCTTCTGTGAAGGAAGAAGAAGTAGCAAGCTTCCCTGGACGTAGAGCATCACCATCGCTTTCTGATACTGGAGTTGTGAGAGGCATTGGTAGAGGTGGCCTCACTGGCCAGATCCCATCTAGCATACCTCTTAGTTCAAGCACTGTGGTTCCTAGTAATAATGCCCTGGGTGCAGTCCCCTCTGCTACTGATGTAGCAAAGAGAAATATTTTGGGAGCTGATGAGAGACTTGGAAGTAGTGGTGTGGTACAGTCTCTTGTTTCCCCTCTGAGCAACCGGATGATCTTGCCTCAGGCTTCCAAACCCAGTGATGGAACTGGGCCAGTTGATTCTAGTAATACAAGTGAGGCTACTGCTATACCTGGCCGAGCATTTTCCCCTTCTATGGTCTCTGGCATGCAATGGAGGCCCGGAAGTTCCAATGAAGCGGGATTGTTTCGTGGAAGAACGGAAATAGCACCTGATCAGAGGGAGAAGTTCTTGCAGCGGCTCCAGCAAGTGCAGCAACAAGGTCACAGTACCATTCTTAGCATGCCCACTCTTGCTGGTGGAAATTCTAAGCAGTTTTCTACTCAGCAGCAGAATCCACTCTTGCAACAGTTTAATTCTGCAAGCTCATCTGTACCTTCTCAAGCTGGCATGGGACTTGGGGTAGGTCTTGGGACTGTTTCATCTACTGCCTTACAGCAGCAGCTAAATTCCATCCACCAACAGTCTCCACAGGCTAATCCACAGGCATTGATGTCAAGTGGACCAAAAGAAGCTGATGCTGGTCATCCAAAAGTTGAGGACCAGCAGCAACAGAATCTTCCTGATGACTCGACATCTGAATCTGCTCCTAGTTCTGGGCTCGTAAAGAATCTAATGAATGAGGATGATATGAAAGCCTCATATGCAATTGATTCTCTGACTGGAGTTTCTAGCTCTTTGACAGAGTCTTCTCAAGTACCTCGAGATATCGATCTCTCTCCTGGGCAACCTTTGCAGTCGAGTCAACCTTCTGCTAGCCTTGGTGTTATTGGCCGAAGAAGTGTTTCTGATCTTGGTGCAATTGGCGATAACCTCTCCGGATCAACTGCTAATTCTGGGGCAATGCATGATCAGTTGTATAATTTGCAGATGCTTGAGGCTGCTTATTACAAACTTCCACAACCCAAAGACTCTGAACGTGCAAGAAGCTACACTCCAAGGCACCCTGCAATAACTCCTCCTAGCTATCCCCAAGTTCAAGCACCTATAGTTAATAATCCTGCTTTCTGGGAACGTTTGGGTTCTGAACAATATTCTACTGATACCCTGTTCTTTGCATTTTACTATCAGCAGAACACTTACCAACAATATTTGGCTGCCAAAGAGTTGAAGAAGCAATCTTGGAGATACCACAGGAAATACAACACTTGGTTTCAACGACACGAAGAGCCAAAAGTTGCTACTGATGAATATGAACAGGGTACATATGTGTACTTTGACTTCCATATTGCCAATGATGATCTACAACATGGATGGTGTCAAAGGATCAAAACCGAGTTTACCTTTGAATATAACTATCTTGAAGATGAACTTATCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

893

Amino Acids

97.56

Weight (kDa)

5.34

Isoelectric Point (pI)

52.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Not3 PF04065 4 - 235 2.3e-83 Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C PF04153 749 - 887 9.7e-34 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 1 cut(s) 2301
AccIII TCCGGA 1 cut(s) 2177
AciI CCGC 4 cut(s) 169, 494, 1538, 1597
AclI AACGTT 2 cut(s) 900, 2374
AclWI GGATC 4 cut(s) 359, 1216, 2188, 2633
AcoI YGGCCR 3 cut(s) 1216, 1474, 2131
AcsI RAATTY 2 cut(s) 1663, 1807
AfiI CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1495, 1955
AflII CTTAAG 1 cut(s) 565
AjnI CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1275, 1471, 2080
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AloI GAACNNNNNNTCC 2 cut(s) 901, 933
Alw21I GWGCWC 2 cut(s) 760, 934
Alw26I GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1830
Alw44I GTGCAC 2 cut(s) 756, 930
AlwI GGATC 4 cut(s) 359, 1216, 2188, 2633
AlwNI CAGNNNCTG 2 cut(s) 250, 1079
Ama87I CYCGRG 1 cut(s) 2061
Aor13HI TCCGGA 1 cut(s) 2177
AoxI GGCC 8 cut(s) 135, 346, 1207, 1216, 1430, 1474, 1517, 2131
ApaLI GTGCAC 2 cut(s) 756, 930
ApoI RAATTY 2 cut(s) 1663, 1807
Asp700I GAANNNNTTC 1 cut(s) 2143
AspS9I GGNCC 7 cut(s) 52, 178, 346, 1430, 1518, 1862, 1900
AsuC2I CCSGG 3 cut(s) 38, 529, 1521
AsuHPI GGTGA 2 cut(s) 238, 1152
AsuNHI GCTAGC 1 cut(s) 2114
AvaI CYCGRG 1 cut(s) 2061
AvaII GGWCC 4 cut(s) 52, 178, 1862, 1900
AxyI CCTNAGG 1 cut(s) 1401
BaeGI GKGCMC 2 cut(s) 760, 934
BaeI ACNNNNGTAYC 4 cut(s) 1167, 1200, 2553, 2586
BalI TGGCCA 1 cut(s) 1218
BanI GGYRCC 1 cut(s) 2301
BanII GRGCYC 1 cut(s) 1962
BarI GAAGNNNNNNTAC 2 cut(s) 2564, 2596
BbsI GAAGAC 3 cut(s) 636, 782, 2040
Bbv12I GWGCWC 2 cut(s) 760, 934
BccI CCATC 6 cut(s) 516, 1171, 1234, 1415, 1820, 2607
BcgI CGANNNNNNTGC 2 cut(s) 1146, 1180
BciT130I CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1277, 1473, 2082
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 2 cut(s) 1573, 2209
BcnI CCSGG 3 cut(s) 38, 529, 1521
BcoDI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1830
BfmI CTRYAG 1 cut(s) 2346
BfrI CTTAAG 1 cut(s) 565
BfuAI ACCTGC 1 cut(s) 592
BfuI GTATCC 1 cut(s) 859
BglI GCCNNNNNGGC 1 cut(s) 1215
BmcAI AGTACT 1 cut(s) 841
Bme18I GGWCC 4 cut(s) 52, 178, 1862, 1900
BmeT110I CYCGRG 1 cut(s) 2061
BmgT120I GGNCC 7 cut(s) 52, 178, 346, 1430, 1518, 1862, 1900
BmiI GGNNCC 4 cut(s) 179, 1260, 1601, 2303
BmrI ACTGGG 3 cut(s) 969, 1410, 1437
BmsI GCATC 8 cut(s) 247, 753, 800, 806, 956, 1166, 1867, 2220
BmtI GCTAGC 1 cut(s) 2118
BmuI ACTGGG 3 cut(s) 969, 1410, 1437
BpiI GAAGAC 3 cut(s) 636, 782, 2040
BplI GAGNNNNNCTC 2 cut(s) 1194, 1226
BpmI CTGGAG 5 cut(s) 5, 149, 1200, 1586, 2043
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 4 cut(s) 29, 1725, 2037, 2255
BpuMI CCSGG 3 cut(s) 38, 529, 1521
Bsa29I ATCGAT 1 cut(s) 2070
BsaBI GATNNNNATC 1 cut(s) 2070
BsaI GGTCTC 2 cut(s) 305, 1504
BsaJI CCNNGG 8 cut(s) 22, 876, 1144, 1275, 1276, 2081, 2119, 2297
BsaWI WCCGGW 2 cut(s) 1386, 2177
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bsc4I CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1495, 1955
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 8 cut(s) 204, 964, 1183, 1219, 1416, 1432, 1433, 2026
Bse21I CCTNAGG 1 cut(s) 1401
Bse3DI GCAATG 2 cut(s) 1517, 2208
Bse8I GATNNNNATC 1 cut(s) 2070
BseAI TCCGGA 1 cut(s) 2177
BseBI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1277, 1473, 2082
BseCI ATCGAT 1 cut(s) 2070
BseDI CCNNGG 8 cut(s) 22, 876, 1144, 1275, 1276, 2081, 2119, 2297
BseGI GGATG 7 cut(s) 73, 262, 1395, 1812, 1885, 1990, 2618
BseJI GATNNNNATC 1 cut(s) 2070
BseLI CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1495, 1955
BseMI GCAATG 2 cut(s) 1517, 2208
BseMII CTCAG 8 cut(s) 444, 446, 588, 604, 1015, 1370, 1415, 1697
BseNI ACTGG 8 cut(s) 204, 964, 1183, 1219, 1416, 1432, 1433, 2026
BseRI GAGGAG 1 cut(s) 2307
BseSI GKGCMC 2 cut(s) 760, 934
BsgI GTGCAG 2 cut(s) 1302, 1631
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 8 cut(s) 137, 348, 1209, 1218, 1432, 1476, 1519, 2133
BshNI GGYRCC 1 cut(s) 2301
BshVI ATCGAT 1 cut(s) 2070
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 760, 934
BsiHKCI CYCGRG 1 cut(s) 2061
BsiSI CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2178
BslFI GGGAC 5 cut(s) 191, 703, 1271, 1767, 1786
BslI CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1495, 1955
BsmAI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1830
BsmFI GGGAC 5 cut(s) 191, 703, 1271, 1767, 1786
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 8 cut(s) 137, 348, 1209, 1218, 1432, 1476, 1519, 2133
Bso31I GGTCTC 2 cut(s) 305, 1504
BsoBI CYCGRG 1 cut(s) 2061
Bsp1286I GDGCHC 3 cut(s) 760, 934, 1962
Bsp13I TCCGGA 1 cut(s) 2177
Bsp1407I TGTACA 1 cut(s) 847
BspACI CCGC 4 cut(s) 169, 494, 1538, 1597
BspANI GGCC 8 cut(s) 137, 348, 1209, 1218, 1432, 1476, 1519, 2133
BspCNI CTCAG 8 cut(s) 445, 445, 589, 603, 1014, 1371, 1414, 1696
BspDI ATCGAT 1 cut(s) 2070
BspEI TCCGGA 1 cut(s) 2177
BspLI GGNNCC 4 cut(s) 179, 1260, 1601, 2303
BspMI ACCTGC 1 cut(s) 592
BspOI GCTAGC 1 cut(s) 2118
BspPI GGATC 4 cut(s) 359, 1216, 2188, 2633
BspQI GCTCTTC 1 cut(s) 2520
BspT107I GGYRCC 1 cut(s) 2301
BspTI CTTAAG 1 cut(s) 565
BspTNI GGTCTC 2 cut(s) 305, 1504
BsrDI GCAATG 2 cut(s) 1517, 2208
BsrFI RCCGGY 1 cut(s) 856
BsrGI TGTACA 1 cut(s) 847
BsrI ACTGG 8 cut(s) 204, 964, 1183, 1219, 1416, 1432, 1433, 2026
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 8 cut(s) 22, 876, 1144, 1275, 1276, 2081, 2119, 2297
BssT1I CCWWGG 2 cut(s) 2119, 2297
Bst2UI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1277, 1473, 2082
Bst6I CTCTTC 1 cut(s) 2520
BstAFI CTTAAG 1 cut(s) 565
BstAPI GCANNNNNTGC 2 cut(s) 1079, 2092
BstAUI TGTACA 1 cut(s) 847
BstC8I GCNNGC 7 cut(s) 246, 1138, 1508, 1643, 1723, 1747, 2116
BstF5I GGATG 7 cut(s) 73, 262, 1395, 1812, 1885, 1990, 2618
BstMAI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1830
BstMCI CGRYCG 1 cut(s) 37
BstNI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1277, 1473, 2082
BstNSI RCATGY 2 cut(s) 1510, 1645
BstSFI CTRYAG 1 cut(s) 2346
BstSLI GKGCMC 2 cut(s) 760, 934
BstV2I GAAGAC 3 cut(s) 636, 782, 2040
BstX2I RGATCY 1 cut(s) 1221
BstYI RGATCY 1 cut(s) 1221
Bsu15I ATCGAT 1 cut(s) 2070
Bsu36I CCTNAGG 1 cut(s) 1401
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 8 cut(s) 137, 348, 1209, 1218, 1432, 1476, 1519, 2133
BsuTUI ATCGAT 1 cut(s) 2070
BtgZI GCGATG 1 cut(s) 1149
BtsCI GGATG 7 cut(s) 73, 262, 1395, 1812, 1885, 1990, 2618
BtsI GCAGTG 1 cut(s) 934
BtsIMutI CAGTG 6 cut(s) 211, 408, 934, 1212, 1251, 1423
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 7 cut(s) 246, 1138, 1508, 1643, 1723, 1747, 2116
CaiI CAGNNNCTG 2 cut(s) 250, 1079
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 7 cut(s) 52, 178, 346, 1430, 1518, 1862, 1900
ClaI ATCGAT 1 cut(s) 2070
CspCI CAANNNNNGTGG 4 cut(s) 1236, 1271, 1830, 1865
CviAII CATG 6 cut(s) 968, 1507, 1642, 1750, 2207, 2609
EaeI YGGCCR 3 cut(s) 1216, 1474, 2131
Eam1104I CTCTTC 1 cut(s) 2520
EarI CTCTTC 1 cut(s) 2520
Eco130I CCWWGG 2 cut(s) 2119, 2297
Eco24I GRGCYC 1 cut(s) 1962
Eco31I GGTCTC 2 cut(s) 305, 1504
Eco32I GATATC 1 cut(s) 2068
Eco47I GGWCC 4 cut(s) 52, 178, 1862, 1900
Eco81I CCTNAGG 1 cut(s) 1401
Eco88I CYCGRG 1 cut(s) 2061
EcoRII CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1275, 1471, 2080
EcoRV GATATC 1 cut(s) 2068
EcoT14I CCWWGG 2 cut(s) 2119, 2297
EcoT22I ATGCAT 1 cut(s) 2208
EcoT38I GRGCYC 1 cut(s) 1962
ErhI CCWWGG 2 cut(s) 2119, 2297
FaeI CATG 6 cut(s) 971, 1510, 1645, 1753, 2210, 2612
FalI AAGNNNNNCTT 1 cut(s) 2655
FaqI GGGAC 5 cut(s) 191, 703, 1271, 1767, 1786
FatI CATG 6 cut(s) 967, 1506, 1641, 1749, 2206, 2608
FauI CCCGC 1 cut(s) 1531
FauNDI CATATG 2 cut(s) 2002, 2566
FbaI TGATCA 2 cut(s) 1573, 2209
FokI GGATG 7 cut(s) 80, 269, 1402, 1799, 1872, 1997, 2625
FriOI GRGCYC 1 cut(s) 1962
GsuI CTGGAG 5 cut(s) 5, 149, 1200, 1586, 2043
HaeIII GGCC 8 cut(s) 137, 348, 1209, 1218, 1432, 1476, 1519, 2133
HapII CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2178
Hin1II CATG 6 cut(s) 971, 1510, 1645, 1753, 2210, 2612
HincII GTYRAC 1 cut(s) 2105
HindII GTYRAC 1 cut(s) 2105
HindIII AAGCTT 3 cut(s) 266, 324, 1138
HpaII CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2178
HphI GGTGA 2 cut(s) 238, 1152
Hpy166II GTNNAC 7 cut(s) 758, 932, 1016, 1862, 2105, 2572, 2640
Hpy8I GTNNAC 7 cut(s) 758, 932, 1016, 1862, 2105, 2572, 2640
HpyCH4IV ACGT 5 cut(s) 643, 900, 1150, 2278, 2374
HpySE526I ACGT 5 cut(s) 643, 900, 1150, 2278, 2374
Hsp92II CATG 6 cut(s) 971, 1510, 1645, 1753, 2210, 2612
Kpn2I TCCGGA 1 cut(s) 2177
Ksp22I TGATCA 2 cut(s) 1573, 2209
LguI GCTCTTC 1 cut(s) 2520
LmnI GCTCC 5 cut(s) 24, 168, 1325, 1605, 1951
LweI GCATC 8 cut(s) 247, 753, 800, 806, 956, 1166, 1867, 2220
MaeII ACGT 5 cut(s) 643, 900, 1150, 2278, 2374
MaeIII GTNAC 2 cut(s) 1060, 1622
MfeI CAATTG 2 cut(s) 2007, 2160
MflI RGATCY 1 cut(s) 1221
MhlI GDGCHC 3 cut(s) 760, 934, 1962
MlsI TGGCCA 1 cut(s) 1218
MluCI AATT 7 cut(s) 1663, 1714, 1807, 2007, 2160, 2191, 2221
MluNI TGGCCA 1 cut(s) 1218
MlyI GAGTC 6 cut(s) 19, 725, 1922, 2054, 2110, 2264
MmeI TCCRAC 2 cut(s) 405, 534
Mox20I TGGCCA 1 cut(s) 1218
Mph1103I ATGCAT 1 cut(s) 2208
MroI TCCGGA 1 cut(s) 2177
MroXI GAANNNNTTC 1 cut(s) 2143
MscI TGGCCA 1 cut(s) 1218
MseI TTAA 5 cut(s) 156, 294, 566, 1713, 2352
Msp20I TGGCCA 1 cut(s) 1218
MspA1I CMGCKG 3 cut(s) 169, 1031, 1597
MspCI CTTAAG 1 cut(s) 565
MspI CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2178
MunI CAATTG 2 cut(s) 2007, 2160
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1277, 1473, 2082
NciI CCSGG 3 cut(s) 38, 529, 1521
NdeI CATATG 2 cut(s) 2002, 2566
NheI GCTAGC 1 cut(s) 2114
NlaIII CATG 6 cut(s) 971, 1510, 1645, 1753, 2210, 2612
NlaIV GGNNCC 4 cut(s) 179, 1260, 1601, 2303
NmeAIII GCCGAG 1 cut(s) 1502
NmuCI GTSAC 2 cut(s) 1060, 1622
NsiI ATGCAT 1 cut(s) 2208
NspI RCATGY 2 cut(s) 1510, 1645
PaeI GCATGC 2 cut(s) 1510, 1645
PaeR7I CTCGAG 1 cut(s) 2061
PasI CCCWGGG 1 cut(s) 1276
PciSI GCTCTTC 1 cut(s) 2520
PctI GAATGC 1 cut(s) 1075
PdmI GAANNNNTTC 1 cut(s) 2143
PfeI GAWTC 9 cut(s) 196, 428, 964, 1439, 1693, 1915, 1940, 1969, 2012
PflFI GACNNNGTC 1 cut(s) 2044
PleI GAGTC 6 cut(s) 18, 724, 1922, 2053, 2109, 2264
PpsI GAGTC 6 cut(s) 18, 724, 1922, 2053, 2109, 2264
Psp1406I AACGTT 2 cut(s) 900, 2374
Psp6I CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1275, 1471, 2080
PspGI CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1275, 1471, 2080
PspN4I GGNNCC 4 cut(s) 179, 1260, 1601, 2303
PspPI GGNCC 7 cut(s) 52, 178, 346, 1430, 1518, 1862, 1900
PstNI CAGNNNCTG 2 cut(s) 250, 1079
PsuI RGATCY 1 cut(s) 1221
PsyI GACNNNGTC 1 cut(s) 2044
PvuII CAGCTG 1 cut(s) 1031
SapI GCTCTTC 1 cut(s) 2520
SaqAI TTAA 5 cut(s) 156, 294, 566, 1713, 2352
Sau96I GGNCC 7 cut(s) 52, 178, 346, 1430, 1518, 1862, 1900
ScaI AGTACT 1 cut(s) 841
SchI GAGTC 6 cut(s) 19, 725, 1922, 2054, 2110, 2264
SduI GDGCHC 3 cut(s) 760, 934, 1962
SfaNI GCATC 8 cut(s) 247, 753, 800, 806, 956, 1166, 1867, 2220
SfcI CTRYAG 1 cut(s) 2346
SfiI GGCCNNNNNGGCC 1 cut(s) 1215
Sfr274I CTCGAG 1 cut(s) 2061
SinI GGWCC 4 cut(s) 52, 178, 1862, 1900
SlaI CTCGAG 1 cut(s) 2061
SmlI CTYRAG 6 cut(s) 44, 565, 1740, 2052, 2061, 2234
SmoI CTYRAG 6 cut(s) 44, 565, 1740, 2052, 2061, 2234
SphI GCATGC 2 cut(s) 1510, 1645
Sse9I AATT 7 cut(s) 1663, 1714, 1807, 2007, 2160, 2191, 2221
SsiI CCGC 4 cut(s) 169, 494, 1538, 1597
SspI AATATT 3 cut(s) 1318, 2393, 2450
StyI CCWWGG 2 cut(s) 2119, 2297
TaiI ACGT 5 cut(s) 646, 903, 1153, 2281, 2377
TaqI TCGA 6 cut(s) 33, 75, 1931, 2062, 2070, 2099
TasI AATT 7 cut(s) 1663, 1714, 1807, 2007, 2160, 2191, 2221
TatI WGTACW 3 cut(s) 839, 847, 2571
TauI GCSGC 1 cut(s) 1600
TfiI GAWTC 9 cut(s) 196, 428, 964, 1439, 1693, 1915, 1940, 1969, 2012
Tru1I TTAA 5 cut(s) 156, 294, 566, 1713, 2352
Tru9I TTAA 5 cut(s) 156, 294, 566, 1713, 2352
TscAI CASTG 6 cut(s) 211, 415, 934, 1219, 1258, 1423
TseFI GTSAC 2 cut(s) 1060, 1622
Tsp45I GTSAC 2 cut(s) 1060, 1622
TspGWI ACGGA 1 cut(s) 1574
TspRI CASTG 6 cut(s) 211, 415, 934, 1219, 1258, 1423
Tth111I GACNNNGTC 1 cut(s) 2044
Vha464I CTTAAG 1 cut(s) 565
VneI GTGCAC 2 cut(s) 756, 930
VpaK11BI GGWCC 4 cut(s) 52, 178, 1862, 1900
XapI RAATTY 2 cut(s) 1663, 1807
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 1510, 1645
XhoI CTCGAG 1 cut(s) 2061
XmnI GAANNNNTTC 1 cut(s) 2143
ZrmI AGTACT 1 cut(s) 841
Zsp2I ATGCAT 1 cut(s) 2208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.