Prupe.5G242100_v2.0.a1

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0006861
Physical Location & Seq
Forward (+)
18212923 .. 18222057
9135 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G242100.1

Sequence Viewer

Length: 2703 bp
ATGGGGGCGAGTCGGAAACTTCAGGGCGAGATCGACCGCGTTCTCAAGAAGGTCCAAGAAGGCGTGGATGTCTTCGATAGCATATGGAACAAGGTTTATGATACGGACAATGCGAACCAGAAGGAAAAGTTTGAGGCGGACCTAAAGAAGGAGATTAAGAAGCTCCAGAGGTACAGGGACCAAATCAAGACTTGGATTCAATCCAGTGAAATCAAGGATAAGAAGGTCAGTGCCTCTTATGAGCAGGCTCTGGTGGATGCTCGCAAGCTTATCGAGCGTGAAATGGAAAGGTTTAAGATTTGTGAAAAGGAGACCAAAACAAAAGCATTTTCTAAAGAAGGCTTGGGCCAACAACCTAAAACCGATCCGAGGGAGAAGGCCAAGTCTGAGACAAGGGATTGGATAAACAATGTGGTTGGGGAGTTGGAATCTCAGATTGACAGCTTTGAAGCTGAGATTGAAGGCCTATCTTTCAGGAAAGGGAAGGGCAGACCACCCAGACTGACCCATCTAGAGACATCTATTACTCGGCATAAGGCTCACATAATGAAGTTAGAACTGATCCTGAGGCTATTGGATAATGATGAACTGAGTCCTGAGCAGGTCAATGATGTCAAAGACTTCTTGGAAGACTATGTTGAACGCAATCAGGAGGATTTTGATGAATTTAGTGAAGTTGATGAGCTTTACAACACCTTACCATTGGACAAAGTGGAGTCCCTTGAAGATCTGGTTACTATTGTCCCTCCTGGTCTTGTCAAGGGTGCACCGGTGCTTGGCTTGAAGACTTCTTTGGCAGTATCCGCATCTCCAATGCCTGCCGCAGCTACTTCCACTACTCAGCAGAGTACTTCTGTACAAGAGCCAGTTGAAGATACAGTTTCCCAGGACAGTAATGTTGATAATATTCCTAGGACTCCACCTCCTAAAAGTAGTGCACTTGCTTCTTCTCCTGCATCAACACCAGTTGGGGGTCTTGCAAGTCCTCTCTCTGTGAGTGTTTCATCTCACAATTTGCCTGGTCCGCCAAGTGTTTCAGCCGTTCCAGGTTCAATTGCTGTTCGTGGTGTCACAGAGAATGCAGGAGCTTCTAATTCTTCATCTCCTGTAAGTCTGTCCGCTTCTGTGAAGGAAGAAGAATTAGCAAGCTTCCCGGGCCGTAGACCATCGCCATCTCTTTCTGATGGTGGGCTTGTGAGGGGCGTTGGTAGAGGTGGCCTCTCTGCACAGAGTCCATCTAGTATCCCTCTTAGTTCTAGCAATGTAGCTCCTAGTAATAGTACCCTTAGTGCAGCTCCTTCGGTTTCTGATGTGACAAAGAGAAATATATTGGGAGCTGATGAGAGAATTGGGAGTAGTAGCGTAGTGCAGCCTCTTGTTTCCCCTATAAGTAACAGATTGATCTTGCCTCAAGCTGCCAAGGCTAGTGATGGAAGTATTCCAGTTGATTCTGGTAATGCTGGTGAGGCTGCTGCTATTCCTGGCAGAGCATTTTCTCCTTCTATGGTCTCCAGCATGCAATGGAGGCCTGGAAGTTCCTTCCAAAACCAGAACGAAGCGGGGTTGTTTCGTGGAAGAACTGAAATAGCACCTGATCAGAGGGAGAAATTTTTGCAGCGGCTCCAACAAGTGCAGCAAGGTCACAGTACCATTCTCGGCATGCCTCCTCTTGCTGGTGGAAATCATAAGCAATTTTCTGGACAGCAGCAAAATCCACTCTTACAACAGTTTAATTCTCAGAACTCATCTGTATCTTCTCAAGCTGGCCTCGGAGTAGGGGTACAAGCCCCAGGTCTTGGTACTGTTGCACCTACCACCTTACAGCAGCAGCTAAATTCTATCCATCAACAGTCTAACCAACAGGCATTGATGTCGAGTGGACCAAAAGAAGCTGATGTTGGTCATCCAAAAGTTGAGGACCAGCAGCAACAACAAAGTACACCTGATGATTCAACTGCGGACTCTACTCCGGTTTCTGGGCTTGTAAAGAATCTAATTAATGAGGATGATTTGAAAGCCTCATATGCAATTGATTCTCTGGCTGGAGTTTCTGGCTCTTCGACAGAGCCTGCTCAAGTACCACGAGATATCGATCTCTCTCCTGGGCAACCTTTACAGCCCAATCAACCTTCTGGTAGTCTTGGTGTTATTGGCCGAAGAAGTGTTTCTGACCTTGGTGCAATTGGTGATAACCTTTCTGGATCAACTCCTAATTCTGGGGGAACGCACGATCAGTTGTATAATTTGCAGATGCTTGAGGCTGCTTATTACAAACTTCCACAACCCAAAGACTCGGAACGTGCAAGGAGCTACACTCCAAGGCACCCTGCAATAACTCCTCCTAGCTACCCCCAAGCACAAGCGCCTATTGTTAACAATCCTGCCTTCTGGGAACGTTTGGGTCTTGAACCGTACGGCACTGATACCCTGTTCTTTGCATTTTACTATCAGCAGAACACTTATCAGCAATATTTGGCAGCCAAAGAGTTGAAGAAGCAATCTTGGAGATACCACAGGAAATATAACACTTGGTTTCAACGACACGAGGAGCCAAAAGTTGCCACTGATGAATATGAACAGGGGACATATGTGTACTTTGACTTCCATATCGCAAATGATGATCTACAACATGGATGGTGTCAAAGGATCAAAACCGAGTTTACTTTTGAATATAACTATCTTGAAGATGAACTTATCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

901

Amino Acids

98.16

Weight (kDa)

5.33

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 1 cut(s) 2322
AccB7I CCANNNNNTGG 1 cut(s) 1796
AccI GTMKAC 1 cut(s) 1162
AccII CGCG 1 cut(s) 39
AciI CCGC 9 cut(s) 37, 137, 804, 822, 1025, 1119, 1559, 1618, 1958
AclI AACGTT 1 cut(s) 2395
AclWI GGATC 4 cut(s) 359, 556, 2209, 2654
AcoI YGGCCR 1 cut(s) 2152
AcsI RAATTY 3 cut(s) 665, 1607, 1834
AcuI CTGAAG 1 cut(s) 5
AfiI CCNNNNNNNGG 8 cut(s) 148, 369, 776, 971, 1673, 1796, 1976, 2216
AgeI ACCGGT 1 cut(s) 769
AjnI CCWGG 8 cut(s) 748, 885, 1018, 1045, 1480, 1528, 1789, 2101
AjuI GAANNNNNNNTTGG 4 cut(s) 776, 808, 1881, 1913
Alw21I GWGCWC 2 cut(s) 769, 940
Alw26I GTCTC 4 cut(s) 305, 383, 509, 1513
Alw44I GTGCAC 2 cut(s) 765, 936
AlwI GGATC 4 cut(s) 359, 556, 2209, 2654
AlwNI CAGNNNCTG 2 cut(s) 250, 2069
Ama87I CYCGRG 1 cut(s) 1153
AoxI GGCC 8 cut(s) 346, 378, 463, 1156, 1216, 1526, 1765, 2152
ApaLI GTGCAC 2 cut(s) 765, 936
ApoI RAATTY 3 cut(s) 665, 1607, 1834
AseI ATTAAT 1 cut(s) 1998
AsiGI ACCGGT 1 cut(s) 769
Asp700I GAANNNNTTC 1 cut(s) 2164
AspA2I CCTAGG 1 cut(s) 911
AspLEI GCGC 1 cut(s) 2365
AspS9I GGNCC 8 cut(s) 52, 139, 178, 346, 1022, 1156, 1880, 1918
AsuC2I CCSGG 2 cut(s) 1154, 1155
AsuHPI GGTGA 2 cut(s) 1475, 2198
AvaI CYCGRG 1 cut(s) 1153
AvaII GGWCC 6 cut(s) 52, 139, 178, 1022, 1880, 1918
AvrII CCTAGG 1 cut(s) 911
AxyI CCTNAGG 1 cut(s) 566
BaeGI GKGCMC 2 cut(s) 769, 940
BanI GGYRCC 1 cut(s) 2322
BarI GAAGNNNNNNTAC 2 cut(s) 2585, 2617
BauI CACGAG 2 cut(s) 2082, 2543
BbsI GAAGAC 3 cut(s) 64, 636, 791
Bbv12I GWGCWC 2 cut(s) 769, 940
BccI CCATC 8 cut(s) 516, 1174, 1178, 1180, 1243, 1424, 1851, 2628
BceAI ACGGC 3 cut(s) 1025, 1143, 2431
BcgI CGANNNNNNTGC 6 cut(s) 253, 287, 1281, 1315, 1854, 1888
BciT130I CCWGG 8 cut(s) 750, 887, 1020, 1047, 1482, 1530, 1791, 2103
BciVI GTATCC 2 cut(s) 811, 1253
BclI TGATCA 1 cut(s) 1594
BcnI CCSGG 2 cut(s) 1154, 1155
BcoDI GTCTC 4 cut(s) 305, 383, 509, 1513
BfaI CTAG 7 cut(s) 512, 912, 1239, 1257, 1272, 1425, 2343
BfoI RGCGCY 1 cut(s) 2366
BfuAI ACCTGC 1 cut(s) 592
BfuI GTATCC 2 cut(s) 811, 1253
BglII AGATCT 1 cut(s) 727
BlnI CCTAGG 1 cut(s) 911
BmcAI AGTACT 1 cut(s) 850
Bme18I GGWCC 6 cut(s) 52, 139, 178, 1022, 1880, 1918
BmeT110I CYCGRG 1 cut(s) 1153
BmgT120I GGNCC 8 cut(s) 52, 139, 178, 346, 1022, 1156, 1880, 1918
BmiI GGNNCC 4 cut(s) 179, 1622, 2324, 2550
BmsI GCATC 4 cut(s) 247, 815, 965, 2241
BpiI GAAGAC 3 cut(s) 64, 636, 791
BplI GAGNNNNNCTC 4 cut(s) 1203, 1235, 2299, 2331
BpmI CTGGAG 3 cut(s) 149, 1495, 2064
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 5 cut(s) 29, 1395, 1743, 2058, 2276
BpuMI CCSGG 2 cut(s) 1154, 1155
Bsa29I ATCGAT 1 cut(s) 2091
BsaBI GATNNNNATC 1 cut(s) 2091
BsaI GGTCTC 2 cut(s) 305, 1513
BsaWI WCCGGW 2 cut(s) 769, 1969
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 1764, 1794
Bsc4I CCNNNNNNNGG 8 cut(s) 148, 369, 776, 971, 1673, 1796, 1976, 2216
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 4 cut(s) 204, 866, 965, 1442
Bse21I CCTNAGG 1 cut(s) 566
Bse3DI GCAATG 2 cut(s) 1267, 1526
Bse8I GATNNNNATC 1 cut(s) 2091
BseBI CCWGG 8 cut(s) 750, 887, 1020, 1047, 1482, 1530, 1791, 2103
BseCI ATCGAT 1 cut(s) 2091
BseGI GGATG 5 cut(s) 73, 262, 1903, 2011, 2639
BseJI GATNNNNATC 1 cut(s) 2091
BseLI CCNNNNNNNGG 8 cut(s) 148, 369, 776, 971, 1673, 1796, 1976, 2216
BseMI GCAATG 2 cut(s) 1267, 1526
BseMII CTCAG 8 cut(s) 378, 444, 446, 557, 581, 588, 854, 1751
BseNI ACTGG 4 cut(s) 204, 866, 965, 1442
BseRI GAGGAG 3 cut(s) 1656, 2328, 2561
BseSI GKGCMC 2 cut(s) 769, 940
BsgI GTGCAG 4 cut(s) 1209, 1311, 1388, 1652
Bsh1236I CGCG 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 8 cut(s) 348, 380, 465, 1158, 1218, 1528, 1767, 2154
BshNI GGYRCC 1 cut(s) 2322
BshTI ACCGGT 1 cut(s) 769
BshVI ATCGAT 1 cut(s) 2091
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 769, 940
BsiHKCI CYCGRG 1 cut(s) 1153
BsiSI CCGG 3 cut(s) 770, 1154, 1970
BsiWI CGTACG 1 cut(s) 2412
BslFI GGGAC 4 cut(s) 191, 703, 728, 2596
BslI CCNNNNNNNGG 8 cut(s) 148, 369, 776, 971, 1673, 1796, 1976, 2216
BsmAI GTCTC 4 cut(s) 305, 383, 509, 1513
BsmFI GGGAC 4 cut(s) 191, 703, 728, 2596
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 8 cut(s) 348, 380, 465, 1158, 1218, 1528, 1767, 2154
Bso31I GGTCTC 2 cut(s) 305, 1513
BsoBI CYCGRG 1 cut(s) 1153
Bsp1286I GDGCHC 2 cut(s) 769, 940
Bsp1407I TGTACA 1 cut(s) 856
BspACI CCGC 9 cut(s) 37, 137, 804, 822, 1025, 1119, 1559, 1618, 1958
BspANI GGCC 8 cut(s) 348, 380, 465, 1158, 1218, 1528, 1767, 2154
BspCNI CTCAG 8 cut(s) 379, 445, 445, 558, 582, 589, 853, 1750
BspDI ATCGAT 1 cut(s) 2091
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 4 cut(s) 179, 1622, 2324, 2550
BspMI ACCTGC 1 cut(s) 592
BspPI GGATC 4 cut(s) 359, 556, 2209, 2654
BspQI GCTCTTC 1 cut(s) 2062
BspT107I GGYRCC 1 cut(s) 2322
BspTNI GGTCTC 2 cut(s) 305, 1513
BsrDI GCAATG 2 cut(s) 1267, 1526
BsrFI RCCGGY 1 cut(s) 769
BsrGI TGTACA 1 cut(s) 856
BsrI ACTGG 4 cut(s) 204, 866, 965, 1442
BssAI RCCGGY 1 cut(s) 769
BssSI CACGAG 2 cut(s) 2082, 2543
BssT1I CCWWGG 4 cut(s) 911, 1419, 2173, 2318
Bst2BI CACGAG 2 cut(s) 2082, 2543
Bst2UI CCWGG 8 cut(s) 750, 887, 1020, 1047, 1482, 1530, 1791, 2103
Bst4CI ACNGT 7 cut(s) 880, 893, 1646, 1728, 1804, 1851, 2412
Bst6I CTCTTC 1 cut(s) 2062
BstAUI TGTACA 1 cut(s) 856
BstC8I GCNNGC 9 cut(s) 246, 262, 266, 819, 1147, 1517, 1661, 1765, 2070
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 5 cut(s) 73, 262, 1903, 2011, 2639
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 2366
BstHHI GCGC 1 cut(s) 2365
BstMAI GTCTC 4 cut(s) 305, 383, 509, 1513
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 8 cut(s) 274, 803, 1024, 1155, 1421, 1466, 1525, 2024
BstNI CCWGG 8 cut(s) 750, 887, 1020, 1047, 1482, 1530, 1791, 2103
BstNSI RCATGY 2 cut(s) 1519, 1663
BstSLI GKGCMC 2 cut(s) 769, 940
BstUI CGCG 1 cut(s) 39
BstV2I GAAGAC 3 cut(s) 64, 636, 791
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
Bsu15I ATCGAT 1 cut(s) 2091
Bsu36I CCTNAGG 1 cut(s) 566
BsuI GTATCC 2 cut(s) 811, 1253
BsuRI GGCC 8 cut(s) 348, 380, 465, 1158, 1218, 1528, 1767, 2154
BsuTUI ATCGAT 1 cut(s) 2091
BtgZI GCGATG 1 cut(s) 1152
BtsCI GGATG 5 cut(s) 73, 262, 1903, 2011, 2639
BtsIMutI CAGTG 4 cut(s) 211, 235, 2418, 2562
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 9 cut(s) 246, 262, 266, 819, 1147, 1517, 1661, 1765, 2070
CaiI CAGNNNCTG 2 cut(s) 250, 2069
CfoI GCGC 1 cut(s) 2365
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 8 cut(s) 52, 139, 178, 346, 1022, 1156, 1880, 1918
Cfr9I CCCGGG 1 cut(s) 1153
ClaI ATCGAT 1 cut(s) 2091
CspAI ACCGGT 1 cut(s) 769
CviAII CATG 3 cut(s) 1516, 1660, 2630
EaeI YGGCCR 1 cut(s) 2152
Eam1104I CTCTTC 1 cut(s) 2062
EarI CTCTTC 1 cut(s) 2062
EciI GGCGGA 2 cut(s) 152, 1014
Eco130I CCWWGG 4 cut(s) 911, 1419, 2173, 2318
Eco147I AGGCCT 2 cut(s) 465, 1528
Eco31I GGTCTC 2 cut(s) 305, 1513
Eco32I GATATC 1 cut(s) 2089
Eco47I GGWCC 6 cut(s) 52, 139, 178, 1022, 1880, 1918
Eco57I CTGAAG 1 cut(s) 5
Eco81I CCTNAGG 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 1153
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRII CCWGG 8 cut(s) 748, 885, 1018, 1045, 1480, 1528, 1789, 2101
EcoRV GATATC 1 cut(s) 2089
EcoT14I CCWWGG 4 cut(s) 911, 1419, 2173, 2318
ErhI CCWWGG 4 cut(s) 911, 1419, 2173, 2318
FaeI CATG 3 cut(s) 1519, 1663, 2633
FalI AAGNNNNNCTT 1 cut(s) 2676
FaqI GGGAC 4 cut(s) 191, 703, 728, 2596
FatI CATG 3 cut(s) 1515, 1659, 2629
FauI CCCGC 1 cut(s) 1552
FauNDI CATATG 3 cut(s) 83, 2023, 2587
FbaI TGATCA 1 cut(s) 1594
FblI GTMKAC 1 cut(s) 1162
FokI GGATG 5 cut(s) 80, 269, 1890, 2018, 2646
FspBI CTAG 7 cut(s) 512, 912, 1239, 1257, 1272, 1425, 2343
GlaI GCGC 1 cut(s) 2364
GsuI CTGGAG 3 cut(s) 149, 1495, 2064
HaeII RGCGCY 1 cut(s) 2366
HaeIII GGCC 8 cut(s) 348, 380, 465, 1158, 1218, 1528, 1767, 2154
HapII CCGG 3 cut(s) 770, 1154, 1970
HhaI GCGC 1 cut(s) 2365
Hin1II CATG 3 cut(s) 1519, 1663, 2633
Hin6I GCGC 1 cut(s) 2363
HinP1I GCGC 1 cut(s) 2363
HincII GTYRAC 1 cut(s) 2374
HindII GTYRAC 1 cut(s) 2374
HindIII AAGCTT 2 cut(s) 266, 1147
HpaI GTTAAC 1 cut(s) 2374
HpaII CCGG 3 cut(s) 770, 1154, 1970
HphI GGTGA 2 cut(s) 1475, 2198
Hpy166II GTNNAC 8 cut(s) 767, 938, 1163, 1880, 1940, 2374, 2593, 2661
Hpy8I GTNNAC 8 cut(s) 767, 938, 1163, 1880, 1940, 2374, 2593, 2661
HpyCH4III ACNGT 7 cut(s) 880, 893, 1646, 1728, 1804, 1851, 2412
HpyCH4IV ACGT 2 cut(s) 2299, 2395
HpyF10VI GCNNNNNNNGC 8 cut(s) 274, 803, 1024, 1155, 1421, 1466, 1525, 2024
HpySE526I ACGT 2 cut(s) 2299, 2395
Hsp92II CATG 3 cut(s) 1519, 1663, 2633
HspAI GCGC 1 cut(s) 2363
Ksp22I TGATCA 1 cut(s) 1594
KspAI GTTAAC 1 cut(s) 2374
LguI GCTCTTC 1 cut(s) 2062
LmnI GCTCC 8 cut(s) 168, 1085, 1273, 1300, 1334, 1626, 2307, 2548
LweI GCATC 4 cut(s) 247, 815, 965, 2241
MaeI CTAG 7 cut(s) 512, 912, 1239, 1257, 1272, 1425, 2343
MaeII ACGT 2 cut(s) 2299, 2395
MaeIII GTNAC 5 cut(s) 733, 1069, 1312, 1391, 1640
MfeI CAATTG 3 cut(s) 1053, 2028, 2181
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 2 cut(s) 769, 940
MlyI GAGTC 7 cut(s) 19, 601, 725, 910, 1240, 1955, 2285
MmeI TCCRAC 2 cut(s) 405, 1648
MroXI GAANNNNTTC 1 cut(s) 2164
MseI TTAA 5 cut(s) 156, 294, 1731, 1998, 2373
MspA1I CMGCKG 1 cut(s) 1618
MspI CCGG 3 cut(s) 770, 1154, 1970
MunI CAATTG 3 cut(s) 1053, 2028, 2181
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 8 cut(s) 750, 887, 1020, 1047, 1482, 1530, 1791, 2103
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 8 cut(s) 274, 803, 1024, 1155, 1421, 1466, 1525, 2024
NciI CCSGG 2 cut(s) 1154, 1155
NdeI CATATG 3 cut(s) 83, 2023, 2587
NlaIII CATG 3 cut(s) 1519, 1663, 2633
NlaIV GGNNCC 4 cut(s) 179, 1622, 2324, 2550
NmeAIII GCCGAG 2 cut(s) 508, 1635
NmuCI GTSAC 3 cut(s) 1069, 1312, 1640
NspI RCATGY 2 cut(s) 1519, 1663
PaeI GCATGC 2 cut(s) 1519, 1663
PceI AGGCCT 2 cut(s) 465, 1528
PciSI GCTCTTC 1 cut(s) 2062
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PctI GAATGC 1 cut(s) 1084
PdmI GAANNNNTTC 1 cut(s) 2164
PfeI GAWTC 6 cut(s) 196, 428, 1448, 1949, 1990, 2033
Pfl23II CGTACG 1 cut(s) 2412
PflMI CCANNNNNTGG 1 cut(s) 1796
PinAI ACCGGT 1 cut(s) 769
PleI GAGTC 7 cut(s) 18, 600, 724, 910, 1239, 1955, 2285
PpsI GAGTC 7 cut(s) 18, 600, 724, 910, 1239, 1955, 2285
PshBI ATTAAT 1 cut(s) 1998
Psp1406I AACGTT 1 cut(s) 2395
Psp6I CCWGG 8 cut(s) 748, 885, 1018, 1045, 1480, 1528, 1789, 2101
PspGI CCWGG 8 cut(s) 748, 885, 1018, 1045, 1480, 1528, 1789, 2101
PspLI CGTACG 1 cut(s) 2412
PspN4I GGNNCC 4 cut(s) 179, 1622, 2324, 2550
PspPI GGNCC 8 cut(s) 52, 139, 178, 346, 1022, 1156, 1880, 1918
PstNI CAGNNNCTG 2 cut(s) 250, 2069
PsuI RGATCY 1 cut(s) 727
SapI GCTCTTC 1 cut(s) 2062
SaqAI TTAA 5 cut(s) 156, 294, 1731, 1998, 2373
Sau96I GGNCC 8 cut(s) 52, 139, 178, 346, 1022, 1156, 1880, 1918
ScaI AGTACT 1 cut(s) 850
SchI GAGTC 7 cut(s) 19, 601, 725, 910, 1240, 1955, 2285
SduI GDGCHC 2 cut(s) 769, 940
SfaNI GCATC 4 cut(s) 247, 815, 965, 2241
SgrAI CRCCGGYG 1 cut(s) 769
SinI GGWCC 6 cut(s) 52, 139, 178, 1022, 1880, 1918
SmaI CCCGGG 1 cut(s) 1155
SmlI CTYRAG 5 cut(s) 44, 1410, 1758, 2073, 2255
SmoI CTYRAG 5 cut(s) 44, 1410, 1758, 2073, 2255
SphI GCATGC 2 cut(s) 1519, 1663
SseBI AGGCCT 2 cut(s) 465, 1528
SsiI CCGC 9 cut(s) 37, 137, 804, 822, 1025, 1119, 1559, 1618, 1958
SspI AATATT 2 cut(s) 907, 2471
SspMI CTAG 7 cut(s) 512, 912, 1239, 1257, 1272, 1425, 2343
StuI AGGCCT 2 cut(s) 465, 1528
StyI CCWWGG 4 cut(s) 911, 1419, 2173, 2318
TaaI ACNGT 7 cut(s) 880, 893, 1646, 1728, 1804, 1851, 2412
TaiI ACGT 2 cut(s) 2302, 2398
TaqI TCGA 6 cut(s) 33, 75, 273, 1874, 2060, 2091
TatI WGTACW 4 cut(s) 848, 856, 1937, 2592
TauI GCSGC 2 cut(s) 824, 1621
TfiI GAWTC 6 cut(s) 196, 428, 1448, 1949, 1990, 2033
Tru1I TTAA 5 cut(s) 156, 294, 1731, 1998, 2373
Tru9I TTAA 5 cut(s) 156, 294, 1731, 1998, 2373
TscAI CASTG 4 cut(s) 211, 235, 2425, 2569
TseFI GTSAC 3 cut(s) 1069, 1312, 1640
Tsp45I GTSAC 3 cut(s) 1069, 1312, 1640
TspDTI ATGAA 8 cut(s) 563, 600, 678, 993, 1089, 2583, 2589, 2703
TspGWI ACGGA 1 cut(s) 119
TspMI CCCGGG 1 cut(s) 1153
TspRI CASTG 4 cut(s) 211, 235, 2425, 2569
Van91I CCANNNNNTGG 1 cut(s) 1796
VneI GTGCAC 2 cut(s) 765, 936
VpaK11BI GGWCC 6 cut(s) 52, 139, 178, 1022, 1880, 1918
VspI ATTAAT 1 cut(s) 1998
XagI CCTNNNNNAGG 1 cut(s) 146
XapI RAATTY 3 cut(s) 665, 1607, 1834
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 1519, 1663
XmaI CCCGGG 1 cut(s) 1153
XmaJI CCTAGG 1 cut(s) 911
XmiI GTMKAC 1 cut(s) 1162
XmnI GAANNNNTTC 1 cut(s) 2164
XspI CTAG 7 cut(s) 512, 912, 1239, 1257, 1272, 1425, 2343
ZrmI AGTACT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.