Rh7BG005000

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006861
Physical Location & Seq
Reverse (-)
316452 .. 324070
7619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG005000.1

Sequence Viewer

Length: 2673 bp
ATGGGGGCGAGCCGGAAGCTCCAGGGCGAGATCGACCGTGTTCTCAAGAAGGTCCAGGAAGGCGTAGATGTGTTCGACAGCATTTGGAATAAGGTTTACGACACAGACAATGCGAACCAGAAGGAGAAGTTTGAGGCCGACTTGAAGAAGGAGATTAAGAAGCTCCAGCGGTACAGGGACCAAATCAAGACTTGGATTCAGTCCAGTGAAATCAAGGATAAGAAGGTGAGTGCCTCATACGAGCAGGCTCTGGTGGATGCTCGGAAGCTTATTGAGCGTGAAATGGAAAGGTTTAAGATATGCGAAAAGGAGACCAAAACCAAAGCTTTTTCTAAAGAGGGATTGGGCCAACAACCAAAAACTGATCCGAAGGAGAAGGCTAAATCAGAGACAAGGGATTGGATAAACACTGTGGTAGGGGAGTTGGAATCTCAGATTGACAGCTTTGAAGCTGAGATTGAGGGGGTATCAGTCAAGAAAGGAAAGGGCAGACCGCCTAGACTGACCCATCTAGAGACATCTATTACCCGGCATAAGGCTCATATAATGAAGTTGGAACTGATCTTAAGGTTATTGGATAATGATGAACTCAGTCCTGAGCAGGTCAATGATGTCAAAGACTTTTTGGAAGACTATGTTGAACGTAATCAGGAGGACTTTGATGAGTTTAGTGAAGTTGATGAGCTTTACAGCACCTTACCACTAGACAAGGTGGAGTCCCTTGAAGATATTGTTCCTCCTGGTCTTGTCAAGGGTGCACCGATGCTTGGCTTGAAGACTTCTTTGGCAGCATCAGCATCTCAAATGCCTCCCATTGTTATTACCACCAACCAACCAAGTACTCCTGTACTAGAGCCGGTTGAGGATACAGTTTCCCAGGATAACAGTAATCCTGATAACGTTGCTAGAACTCCACCTCCTAAAAGCAGTGCACTTGGTTCTGCTGCATCAACACCAACTGGGAATCAGGCAACTCCTGTCTCTTTGAATGTTTCATCTCTCAGTTTGCCTGGTGTACCAACTGTTTCAGCTGTTGCTGGTTCAAATGCTGTTCGTGGTGTCACAGAGAATGCAGGGGCTGCTCTTTCTTCATCTCCTGTAAGTCTGTCTCCTTCTGTGAAGGAAGAAGAAGTTGCAAGCTTCCCTGGACGTAGACCATCACCATCGCTTTCTGATACTGGAGTTGTGAGAGGCATTGGTAGAGGTGGCCTCACTGGCCAGATCCCATCTAGCATACCTCTTAGTTCTAGCACTGTGGTTCCTAGTAATAATGCCCTGGGTGCAGTCCCCTCTGCTACTGATGTAGCAAAGAGAAATATTTTGGGAGTTGATGAGAGACTTGGAAGTAGTGGTGTGGTACAGTCTCTTGTTTCCCCTCTGAGTAACCGGATGATCTTGCCTCAGGCTTCCAAACCCAGTGATGGAACTGGGCCAGTTGATTCTAGTAATACAAGTTCTATGGTCTCTGGCATGCAATGGAGGCCCGGAAGTTCCTTCCCAAACCAGAATGAAGCGGGATTGTTTCGTGGAAGAACGGAAATAGCACCTGATCAGAGGGAGAAGTTCTTGCAGCGGCTCCAGCAAGTGCAGCAACAAGGTCACAGTACCATTCTTAGCATGCCCACTCTTGCTGGTGGAAATTCTAAGCAGTTTTCTACTCAGCAGCAGAATCCACTCTTGCAACAGTTTAATTCTCAAAGCTCATCTGTACCTTCTCAAGCTGGCATGGGACTTGGGGTACAGGCACCAGGTCTTGGGACTGTTTCATCTACTACCTTACAGCAGCAGCTAAATTCCATCCATCAACAGTCTCCACAGGCTAATCCACAGGCATTGATGTCAAGTGGACCAAAAGAAGCTGATGCTGGTCATCCAAAAGTTGAGGACCAGCAGCAACAGAATCTTCCTGATGACTCGACATCTGAATCTGCTCCTAGTTCTGGGCTCGTAAAGAATCTAATGAATGAGGATGATATGAAAGCCTCATATGCAATTGATTCTCTGACTGGAGTTTCTAGCTCTTTGACAGAGTCTTCTCAAGTACCACGAGATATCGATCTCTCTCCTGGGCAACCTTTGCAGTCGAGTCAACCTTCTGCTAGCCTTGGTGTTATTGGCCGAAGAAGTGTTTCTGATCTTGGTGCAATTGGCGATAACCTCTCCGGATCAACTGCTAATTCTGGGGCAATGCATGATCAGTTGTATAATTTGCAGATGCTTGAGGCTGCTTATTACAAACTTCCACAACCCAAAGACTCCGAACGTGCAAGAAGCTACACTCCAAGGCACCCTGCAATCACTCCTCCTAGCTATCCCCAAGTTCAAGCACCTATAGTTAATAATCCTGCTTTCTGGGAACGTTTGGGTTCTGAACAGTATTCTACTGATACCCTGTTCTTTGCATTTTACTATCAGCAGAACACTTACCAGCAATATTTGGCTGCCAAAGAGTTGAAGAAGCAATCTTGGAGATACCACAGGAAATACAACACTTGGTTTCAACGACACGAAGAGCCAAAAGTTGCTACTGATGAATATGAACAGGGTACATATGTGTACTTTGACTTCCATATTGCCAATGATGAGCTACAACATGGATGGTACAATTCTATTGATCTTTTGAGTTTTACTTTTTGCAGCCAGTCACATTTAGAGTTTGTATCTGGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

890

Amino Acids

97.18

Weight (kDa)

5.32

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Not3 PF04065 4 - 235 2.3e-83 Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C PF04153 744 - 857 1.6e-29 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 2 cut(s) 1744, 2286
AccB7I CCANNNNNTGG 1 cut(s) 1754
AccI GTMKAC 1 cut(s) 1153
AccIII TCCGGA 1 cut(s) 2162
AciI CCGC 4 cut(s) 169, 494, 1514, 1573
AclI AACGTT 2 cut(s) 900, 2359
AclWI GGATC 3 cut(s) 359, 1216, 2173
AcoI YGGCCR 2 cut(s) 1216, 2116
AcsI RAATTY 2 cut(s) 1639, 1792
AfiI CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1754, 1940
AflII CTTAAG 1 cut(s) 565
AjnI CCWGG 9 cut(s) 21, 54, 739, 876, 1009, 1144, 1275, 1747, 2065
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AloI GAACNNNNNNTCC 2 cut(s) 901, 933
Alw21I GWGCWC 2 cut(s) 760, 934
Alw26I GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1468, 1815
Alw44I GTGCAC 2 cut(s) 756, 930
AlwI GGATC 3 cut(s) 359, 1216, 2173
AlwNI CAGNNNCTG 2 cut(s) 250, 1079
Aor13HI TCCGGA 1 cut(s) 2162
AoxI GGCC 7 cut(s) 135, 346, 1207, 1216, 1430, 1481, 2116
ApaLI GTGCAC 2 cut(s) 756, 930
ApoI RAATTY 2 cut(s) 1639, 1792
Asp700I GAANNNNTTC 1 cut(s) 2128
AspS9I GGNCC 7 cut(s) 52, 178, 346, 1430, 1482, 1847, 1885
AsuC2I CCSGG 2 cut(s) 529, 1485
AsuHPI GGTGA 2 cut(s) 238, 1152
AsuNHI GCTAGC 1 cut(s) 2099
AvaII GGWCC 4 cut(s) 52, 178, 1847, 1885
AxyI CCTNAGG 1 cut(s) 1401
BaeGI GKGCMC 2 cut(s) 760, 934
BaeI ACNNNNGTAYC 4 cut(s) 1167, 1200, 2538, 2571
BalI TGGCCA 1 cut(s) 1218
BanI GGYRCC 2 cut(s) 1744, 2286
BanII GRGCYC 1 cut(s) 1947
BarI GAAGNNNNNNTAC 2 cut(s) 2549, 2581
BauI CACGAG 1 cut(s) 2046
BbsI GAAGAC 3 cut(s) 636, 782, 2025
Bbv12I GWGCWC 2 cut(s) 760, 934
BccI CCATC 8 cut(s) 516, 1165, 1171, 1234, 1415, 1805, 1809, 2592
BciT130I CCWGG 9 cut(s) 23, 56, 741, 878, 1011, 1146, 1277, 1749, 2067
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 2 cut(s) 1549, 2194
BcnI CCSGG 2 cut(s) 529, 1485
BcoDI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1468, 1815
BfmI CTRYAG 1 cut(s) 2331
BfrI CTTAAG 1 cut(s) 565
BfuAI ACCTGC 1 cut(s) 592
BfuI GTATCC 1 cut(s) 859
BglI GCCNNNNNGGC 1 cut(s) 1215
BmcAI AGTACT 1 cut(s) 841
Bme18I GGWCC 4 cut(s) 52, 178, 1847, 1885
BmgT120I GGNCC 7 cut(s) 52, 178, 346, 1430, 1482, 1847, 1885
BmiI GGNNCC 5 cut(s) 179, 1260, 1577, 1746, 2288
BmrI ACTGGG 3 cut(s) 969, 1410, 1437
BmsI GCATC 7 cut(s) 247, 753, 800, 806, 956, 1852, 2205
BmtI GCTAGC 1 cut(s) 2103
BmuI ACTGGG 3 cut(s) 969, 1410, 1437
BpiI GAAGAC 3 cut(s) 636, 782, 2025
BplI GAGNNNNNCTC 2 cut(s) 1194, 1226
BpmI CTGGAG 5 cut(s) 5, 149, 1200, 1562, 2028
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 4 cut(s) 29, 1701, 2022, 2240
BpuMI CCSGG 2 cut(s) 529, 1485
Bsa29I ATCGAT 1 cut(s) 2055
BsaBI GATNNNNATC 1 cut(s) 2055
BsaI GGTCTC 2 cut(s) 305, 1468
BsaJI CCNNGG 8 cut(s) 22, 876, 1144, 1275, 1276, 2066, 2104, 2282
BsaWI WCCGGW 2 cut(s) 1386, 2162
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bsc4I CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1754, 1940
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 9 cut(s) 204, 964, 1183, 1219, 1416, 1432, 1433, 2011, 2641
Bse21I CCTNAGG 1 cut(s) 1401
Bse3DI GCAATG 2 cut(s) 1481, 2193
Bse8I GATNNNNATC 1 cut(s) 2055
BseAI TCCGGA 1 cut(s) 2162
BseBI CCWGG 9 cut(s) 23, 56, 741, 878, 1011, 1146, 1277, 1749, 2067
BseCI ATCGAT 1 cut(s) 2055
BseDI CCNNGG 8 cut(s) 22, 876, 1144, 1275, 1276, 2066, 2104, 2282
BseGI GGATG 6 cut(s) 262, 1395, 1797, 1870, 1975, 2603
BseJI GATNNNNATC 1 cut(s) 2055
BseLI CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1754, 1940
BseMI GCAATG 2 cut(s) 1481, 2193
BseMII CTCAG 8 cut(s) 444, 446, 588, 604, 1015, 1370, 1415, 1673
BseNI ACTGG 9 cut(s) 204, 964, 1183, 1219, 1416, 1432, 1433, 2011, 2641
BseRI GAGGAG 1 cut(s) 2292
BseSI GKGCMC 2 cut(s) 760, 934
BsgI GTGCAG 2 cut(s) 1302, 1607
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 7 cut(s) 137, 348, 1209, 1218, 1432, 1483, 2118
BshNI GGYRCC 2 cut(s) 1744, 2286
BshVI ATCGAT 1 cut(s) 2055
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 760, 934
BsiSI CCGG 6 cut(s) 13, 529, 857, 1387, 1485, 2163
BslFI GGGAC 5 cut(s) 191, 703, 1271, 1743, 1771
BslI CCNNNNNNNGG 5 cut(s) 535, 767, 1421, 1754, 1940
BsmAI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1468, 1815
BsmFI GGGAC 5 cut(s) 191, 703, 1271, 1743, 1771
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 7 cut(s) 137, 348, 1209, 1218, 1432, 1483, 2118
Bso31I GGTCTC 2 cut(s) 305, 1468
Bsp1286I GDGCHC 3 cut(s) 760, 934, 1947
Bsp13I TCCGGA 1 cut(s) 2162
BspACI CCGC 4 cut(s) 169, 494, 1514, 1573
BspANI GGCC 7 cut(s) 137, 348, 1209, 1218, 1432, 1483, 2118
BspCNI CTCAG 8 cut(s) 445, 445, 589, 603, 1014, 1371, 1414, 1672
BspDI ATCGAT 1 cut(s) 2055
BspEI TCCGGA 1 cut(s) 2162
BspLI GGNNCC 5 cut(s) 179, 1260, 1577, 1746, 2288
BspMI ACCTGC 1 cut(s) 592
BspOI GCTAGC 1 cut(s) 2103
BspPI GGATC 3 cut(s) 359, 1216, 2173
BspQI GCTCTTC 1 cut(s) 2505
BspT107I GGYRCC 2 cut(s) 1744, 2286
BspTI CTTAAG 1 cut(s) 565
BspTNI GGTCTC 2 cut(s) 305, 1468
BsrDI GCAATG 2 cut(s) 1481, 2193
BsrFI RCCGGY 1 cut(s) 856
BsrI ACTGG 9 cut(s) 204, 964, 1183, 1219, 1416, 1432, 1433, 2011, 2641
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 8 cut(s) 22, 876, 1144, 1275, 1276, 2066, 2104, 2282
BssSI CACGAG 1 cut(s) 2046
BssT1I CCWWGG 2 cut(s) 2104, 2282
Bst2BI CACGAG 1 cut(s) 2046
Bst2UI CCWGG 9 cut(s) 23, 56, 741, 878, 1011, 1146, 1277, 1749, 2067
Bst6I CTCTTC 1 cut(s) 2505
BstAFI CTTAAG 1 cut(s) 565
BstAPI GCANNNNNTGC 2 cut(s) 1079, 2077
BstC8I GCNNGC 7 cut(s) 10, 246, 1138, 1472, 1619, 1723, 2101
BstF5I GGATG 6 cut(s) 262, 1395, 1797, 1870, 1975, 2603
BstMAI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1468, 1815
BstMCI CGRYCG 1 cut(s) 37
BstNI CCWGG 9 cut(s) 23, 56, 741, 878, 1011, 1146, 1277, 1749, 2067
BstNSI RCATGY 2 cut(s) 1474, 1621
BstSFI CTRYAG 1 cut(s) 2331
BstSLI GKGCMC 2 cut(s) 760, 934
BstV2I GAAGAC 3 cut(s) 636, 782, 2025
BstX2I RGATCY 1 cut(s) 1221
BstYI RGATCY 1 cut(s) 1221
Bsu15I ATCGAT 1 cut(s) 2055
Bsu36I CCTNAGG 1 cut(s) 1401
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 7 cut(s) 137, 348, 1209, 1218, 1432, 1483, 2118
BsuTUI ATCGAT 1 cut(s) 2055
BtgZI GCGATG 1 cut(s) 1149
BtsCI GGATG 6 cut(s) 262, 1395, 1797, 1870, 1975, 2603
BtsI GCAGTG 1 cut(s) 934
BtsIMutI CAGTG 6 cut(s) 211, 408, 934, 1212, 1251, 1423
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 7 cut(s) 10, 246, 1138, 1472, 1619, 1723, 2101
CaiI CAGNNNCTG 2 cut(s) 250, 1079
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 7 cut(s) 52, 178, 346, 1430, 1482, 1847, 1885
ClaI ATCGAT 1 cut(s) 2055
CsiI ACCWGGT 1 cut(s) 1747
CspCI CAANNNNNGTGG 2 cut(s) 1815, 1850
CviAII CATG 5 cut(s) 1471, 1618, 1726, 2192, 2594
EaeI YGGCCR 2 cut(s) 1216, 2116
Eam1104I CTCTTC 1 cut(s) 2505
EarI CTCTTC 1 cut(s) 2505
Eco130I CCWWGG 2 cut(s) 2104, 2282
Eco24I GRGCYC 1 cut(s) 1947
Eco31I GGTCTC 2 cut(s) 305, 1468
Eco32I GATATC 1 cut(s) 2053
Eco47I GGWCC 4 cut(s) 52, 178, 1847, 1885
Eco81I CCTNAGG 1 cut(s) 1401
EcoRII CCWGG 9 cut(s) 21, 54, 739, 876, 1009, 1144, 1275, 1747, 2065
EcoRV GATATC 1 cut(s) 2053
EcoT14I CCWWGG 2 cut(s) 2104, 2282
EcoT22I ATGCAT 1 cut(s) 2193
EcoT38I GRGCYC 1 cut(s) 1947
ErhI CCWWGG 2 cut(s) 2104, 2282
FaeI CATG 5 cut(s) 1474, 1621, 1729, 2195, 2597
FaqI GGGAC 5 cut(s) 191, 703, 1271, 1743, 1771
FatI CATG 5 cut(s) 1470, 1617, 1725, 2191, 2593
FauI CCCGC 1 cut(s) 1507
FauNDI CATATG 2 cut(s) 1987, 2551
FbaI TGATCA 2 cut(s) 1549, 2194
FblI GTMKAC 1 cut(s) 1153
FokI GGATG 6 cut(s) 269, 1402, 1784, 1857, 1982, 2610
FriOI GRGCYC 1 cut(s) 1947
GsuI CTGGAG 5 cut(s) 5, 149, 1200, 1562, 2028
HaeIII GGCC 7 cut(s) 137, 348, 1209, 1218, 1432, 1483, 2118
HapII CCGG 6 cut(s) 13, 529, 857, 1387, 1485, 2163
Hin1II CATG 5 cut(s) 1474, 1621, 1729, 2195, 2597
HincII GTYRAC 1 cut(s) 2090
HindII GTYRAC 1 cut(s) 2090
HindIII AAGCTT 3 cut(s) 266, 324, 1138
HpaII CCGG 6 cut(s) 13, 529, 857, 1387, 1485, 2163
HphI GGTGA 2 cut(s) 238, 1152
Hpy166II GTNNAC 8 cut(s) 97, 758, 932, 1016, 1154, 1847, 2090, 2557
Hpy8I GTNNAC 8 cut(s) 97, 758, 932, 1016, 1154, 1847, 2090, 2557
HpyCH4IV ACGT 5 cut(s) 643, 900, 1150, 2263, 2359
HpySE526I ACGT 5 cut(s) 643, 900, 1150, 2263, 2359
Hsp92II CATG 5 cut(s) 1474, 1621, 1729, 2195, 2597
Kpn2I TCCGGA 1 cut(s) 2162
Ksp22I TGATCA 2 cut(s) 1549, 2194
LguI GCTCTTC 1 cut(s) 2505
LmnI GCTCC 4 cut(s) 24, 168, 1581, 1936
LweI GCATC 7 cut(s) 247, 753, 800, 806, 956, 1852, 2205
MabI ACCWGGT 1 cut(s) 1747
MaeII ACGT 5 cut(s) 643, 900, 1150, 2263, 2359
MaeIII GTNAC 4 cut(s) 1060, 1382, 1598, 2643
MfeI CAATTG 2 cut(s) 1992, 2145
MflI RGATCY 1 cut(s) 1221
MhlI GDGCHC 3 cut(s) 760, 934, 1947
MlsI TGGCCA 1 cut(s) 1218
MluCI AATT 8 cut(s) 1639, 1690, 1792, 1992, 2145, 2176, 2206, 2605
MluNI TGGCCA 1 cut(s) 1218
MlyI GAGTC 5 cut(s) 725, 1907, 2039, 2095, 2249
MmeI TCCRAC 2 cut(s) 405, 534
Mox20I TGGCCA 1 cut(s) 1218
Mph1103I ATGCAT 1 cut(s) 2193
MroI TCCGGA 1 cut(s) 2162
MroXI GAANNNNTTC 1 cut(s) 2128
MscI TGGCCA 1 cut(s) 1218
MseI TTAA 5 cut(s) 156, 294, 566, 1689, 2337
Msp20I TGGCCA 1 cut(s) 1218
MspA1I CMGCKG 3 cut(s) 169, 1031, 1573
MspCI CTTAAG 1 cut(s) 565
MspI CCGG 6 cut(s) 13, 529, 857, 1387, 1485, 2163
MunI CAATTG 2 cut(s) 1992, 2145
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 9 cut(s) 23, 56, 741, 878, 1011, 1146, 1277, 1749, 2067
NciI CCSGG 2 cut(s) 529, 1485
NdeI CATATG 2 cut(s) 1987, 2551
NheI GCTAGC 1 cut(s) 2099
NlaIII CATG 5 cut(s) 1474, 1621, 1729, 2195, 2597
NlaIV GGNNCC 5 cut(s) 179, 1260, 1577, 1746, 2288
NmuCI GTSAC 3 cut(s) 1060, 1598, 2643
NsiI ATGCAT 1 cut(s) 2193
NspI RCATGY 2 cut(s) 1474, 1621
PaeI GCATGC 2 cut(s) 1474, 1621
PasI CCCWGGG 1 cut(s) 1276
PciSI GCTCTTC 1 cut(s) 2505
PctI GAATGC 1 cut(s) 1075
PdmI GAANNNNTTC 1 cut(s) 2128
PfeI GAWTC 9 cut(s) 196, 428, 964, 1439, 1669, 1900, 1925, 1954, 1997
PflFI GACNNNGTC 1 cut(s) 2029
PflMI CCANNNNNTGG 1 cut(s) 1754
PfoI TCCNGGA 1 cut(s) 54
PleI GAGTC 5 cut(s) 724, 1907, 2038, 2094, 2249
PpsI GAGTC 5 cut(s) 724, 1907, 2038, 2094, 2249
Psp1406I AACGTT 2 cut(s) 900, 2359
Psp6I CCWGG 9 cut(s) 21, 54, 739, 876, 1009, 1144, 1275, 1747, 2065
PspGI CCWGG 9 cut(s) 21, 54, 739, 876, 1009, 1144, 1275, 1747, 2065
PspN4I GGNNCC 5 cut(s) 179, 1260, 1577, 1746, 2288
PspPI GGNCC 7 cut(s) 52, 178, 346, 1430, 1482, 1847, 1885
PsrI GAACNNNNNNTAC 2 cut(s) 1438, 1470
PstNI CAGNNNCTG 2 cut(s) 250, 1079
PsuI RGATCY 1 cut(s) 1221
PsyI GACNNNGTC 1 cut(s) 2029
PvuII CAGCTG 1 cut(s) 1031
SapI GCTCTTC 1 cut(s) 2505
SaqAI TTAA 5 cut(s) 156, 294, 566, 1689, 2337
Sau96I GGNCC 7 cut(s) 52, 178, 346, 1430, 1482, 1847, 1885
ScaI AGTACT 1 cut(s) 841
SchI GAGTC 5 cut(s) 725, 1907, 2039, 2095, 2249
SduI GDGCHC 3 cut(s) 760, 934, 1947
SexAI ACCWGGT 1 cut(s) 1747
SfaNI GCATC 7 cut(s) 247, 753, 800, 806, 956, 1852, 2205
SfcI CTRYAG 1 cut(s) 2331
SfiI GGCCNNNNNGGCC 1 cut(s) 1215
SinI GGWCC 4 cut(s) 52, 178, 1847, 1885
SmlI CTYRAG 5 cut(s) 44, 565, 1716, 2037, 2219
SmoI CTYRAG 5 cut(s) 44, 565, 1716, 2037, 2219
SphI GCATGC 2 cut(s) 1474, 1621
Sse9I AATT 8 cut(s) 1639, 1690, 1792, 1992, 2145, 2176, 2206, 2605
SsiI CCGC 4 cut(s) 169, 494, 1514, 1573
SspI AATATT 2 cut(s) 1318, 2435
StyI CCWWGG 2 cut(s) 2104, 2282
TaiI ACGT 5 cut(s) 646, 903, 1153, 2266, 2362
TaqI TCGA 5 cut(s) 33, 75, 1916, 2055, 2084
TasI AATT 8 cut(s) 1639, 1690, 1792, 1992, 2145, 2176, 2206, 2605
TatI WGTACW 3 cut(s) 839, 847, 2556
TauI GCSGC 1 cut(s) 1576
TfiI GAWTC 9 cut(s) 196, 428, 964, 1439, 1669, 1900, 1925, 1954, 1997
Tru1I TTAA 5 cut(s) 156, 294, 566, 1689, 2337
Tru9I TTAA 5 cut(s) 156, 294, 566, 1689, 2337
TscAI CASTG 6 cut(s) 211, 415, 934, 1219, 1258, 1423
TseFI GTSAC 3 cut(s) 1060, 1598, 2643
Tsp45I GTSAC 3 cut(s) 1060, 1598, 2643
TspGWI ACGGA 1 cut(s) 1550
TspRI CASTG 6 cut(s) 211, 415, 934, 1219, 1258, 1423
Tth111I GACNNNGTC 1 cut(s) 2029
Van91I CCANNNNNTGG 1 cut(s) 1754
Vha464I CTTAAG 1 cut(s) 565
VneI GTGCAC 2 cut(s) 756, 930
VpaK11BI GGWCC 4 cut(s) 52, 178, 1847, 1885
XapI RAATTY 2 cut(s) 1639, 1792
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 1474, 1621
XmiI GTMKAC 1 cut(s) 1153
XmnI GAANNNNTTC 1 cut(s) 2128
ZrmI AGTACT 1 cut(s) 841
Zsp2I ATGCAT 1 cut(s) 2193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.