MD06G1237800.v1.1

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006861
Physical Location & Seq
Forward (+)
36885338 .. 36897952
12615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1237800.v1.1.491

Sequence Viewer

Length: 2694 bp
ATGGGGGCGAGTCGGAAGCTCCAGGGCGAGATCGACCGCGTTCTCAAGAAGGTCCAAGAAGGCGTCGACGTCTTCGATAGCATTTGGAACAAGGTTTACGATACGGACAATGCGAACCAGAAAGAGAAGTTTGAGGCCGACCTCAAGAAGGAGATTAAGAAGCTTCAGAGGTACAGGGACCAGATCAAGACTTGGATTCAATCCAGTGAAATCAAGGATAAAAAGGTCAGTGCCTCTTATGAGCAGGCTCTGGTGGATGCTCGCAAACTCATTGAGCGCGAAATGGAAAGGTTCAAGATATGTGAAAAGGAGACAAAAACAAAAGCATTTTCTAAAGAAGGATTGGGCCAACAACCTAAAACCGATCCGAGGGAGAAGGCCAAATCAGAGACAAGGGATTGGATAAACAACGTGGTTGGGGAGTTGGAGTCTCAGATTGACAGCTTTGAAGCTGAGATTGAAGGGCTATCTTTTAAGAAAGGGAAGGGCAGACCACCCAGACTGACCCATCTAGAGACATCAATTACTCGGCATAAGGCTCATATAATGAAGTTAGAACTGATCTTGAGGCTATTGGATAATGATGAGCTGAGTCCTGAGCAGGTCAATGATGTTAAAGACTTTTTGGAAGACTATGTGGAACGCAATCAGGAGGATTTTGATGAATTTAGTGAAGTTGATGAGCTTTACAACACCTTACCATTGGACAAGGTGGAGTCCCTTGAAGATCTGGCCATTATTCCTCCTGGTCTTATCAAGGGTGCACCGATGCTTGGCTTGAAGACTAATTTGGCACCACCCGCATCTCCACTGCCTCCCGCTGCTACTTCTACTAATCAGCAGAGTGTTTCTGTCCAAGAGCCAGTTGAAGATGCAGTTTCCCAGGACAGTAATGCTGATAACATTCCTAGAACTCCACCTCCTAGAAGTGGTGTGCTTTCATCTTCTCCTGCATCAACACCAACTGGGAGTCATGCAACTCCTGTCTCTGCAAGTGTTCCAACTCATAATCTGCCCAGTGTGCCAAGTGTTTTGGCCATTCCAGGTTCAAATGCTGTTCGGGGTGTCACAGAGAATGCAGGGGCTGCTAATTCTTCATCTCCTGTAAGTTTGTCTGCTTCTGTGAAGGAAGAAGAGTCAGCAAGCTTCCCTGGCCGTAGACCATCACCATCTCTTTCTGATGCTGGACTTGTAAGGGGCATCAGTCGAGGTGGCTTCTCTGCCCCAATTCCATCTAGTATCCCTGTTAGCTCTAGCAATGTGTCTCCTAGTAATAGTGCCCTTGGTGCAGTTCCTTCAGTTTCTGATGTGACCAAGAGAAATATATTGGGAGCTGATGAGAGAATTGGGAATAGTGGTGTGGTGCAGCCACTTGTTTCCCCTATAACTAACAGACTGATCTTGCCTCAAGCTGCCAAGGCTAGTGATGGAAGTGCTCCAGTTGATTCTAGTAATGCTAGTGAGGCTGCTATACCTGGCAGAGCATTTTCTCCTTCTATTGTCTCTGGCATGCAGTGGAGGCCTGGAAGTTCCTTTCAAAACCAAAATGAAGCAGGGATATTTCGTGGAAGGACTGAAATAGCACCCGATCAGAGGGAGAAATTTTTGCAGCGGCTCCAACAAGTGCAGCAACAAGGTCACAGTACCATTCTTGGCATGCCTCCTCTTGCTGGTGGAAATCATAAGCAATTTTCTGCACAGCAAAACCCACTTTTGCAACAGTTTAATCCTCAAAACTCATCTGTATCTTCTCAAGCTGGCCCTGGACTAGGGGTACAGCCCCCAGGTCTTGGTACTGTTGCACCTACCACCTTACAGCAGCAGCTAAATTCTATCCATCAACAGTCTAACCAACAGGCATTGATGTCGGTTGGACCAAAAGAAACTGATGTAAGTCATCCAAAAGTTGAGGAACAACAGCAACAACAGAATATACCTGATGAGTCTACTGCGGACTCTACTAGTTCTGGACTCGTAAAGAATCTAATGAATGAAGATGACTTGAAAGCCTCATATGCAGTTGATTCTCTGGCTGGAGTTTCTGGCTCTTCAACAGAGCCTGCTCAAGTACCACGAGATATTGATCTCTCTCCTGGGCAACCTTTACAGTCCAATCAACCTTCTCCTAGCCTTGGCATTATTGGCCGAAGAAGTGTTTCTGATCTTGGTGCAATTGGTGATAACCTTTCTGGATCAACTGCTAATTCTGGGGTATTGCATGATCAGTCGTATAATTCGCAGATGCTTGAGGCTGCTTACTACAAACTTCCACAACCCAAAGACTCAGAACGTGCAAGGAGCTATACTCCAAGGCACCCTGCAAAAACACCTCTAAGCTACCCCCAAGCGCAAGCACCTATTGTTAATAATCCTGCCTTCTGGGAACGGTTAGGTCTTGAACCATATGGCACTGATACCCTGTTCTTTGCATTTTACTATCAGCAGAACACTTATCAGCAATATTTGGCGGCAAAAGAACTGAAGAAGCAATCTTGGAGATACCATAGGAAATATAACACTTGGTTTCAACGGCACGAGGAGCCAAAAGTTGCCACAGATGAATATGAACAGGGGACGTATGTGTACTTTGACTTTCATATTGCAAATGATGATCTACAACACGGATGGTGCCAAAGGATCAAAACTGAGTTCACTTTTGAATATAACTATCTTGAGGATGAGCTTATCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

898

Amino Acids

98.29

Weight (kDa)

5.41

Isoelectric Point (pI)

53.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Not3 PF04065 4 - 235 2.3e-83 Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C PF04153 753 - 891 5.1e-34 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 3 cut(s) 793, 2313, 2628
AccB7I CCANNNNNTGG 1 cut(s) 1790
AccI GTMKAC 3 cut(s) 66, 1159, 1946
AccII CGCG 2 cut(s) 39, 279
AciI CCGC 6 cut(s) 37, 801, 819, 1612, 1952, 2468
AclWI GGATC 3 cut(s) 359, 2200, 2645
AcoI YGGCCR 4 cut(s) 732, 1035, 1153, 2143
AcsI RAATTY 3 cut(s) 665, 1601, 1828
AcuI CTGAAG 3 cut(s) 149, 1281, 2501
AcyI GRCGYC 2 cut(s) 63, 69
AfaI GTAC 6 cut(s) 173, 1645, 1776, 1795, 2070, 2585
AfiI CCNNNNNNNGG 9 cut(s) 148, 369, 773, 929, 1593, 1670, 1769, 1790, 2132
AhlI ACTAGT 1 cut(s) 1961
AjuI GAANNNNNNNTTGG 2 cut(s) 773, 805
AloI GAACNNNNNNTCC 2 cut(s) 904, 936
Alw21I GWGCWC 2 cut(s) 766, 1438
Alw26I GTCTC 7 cut(s) 305, 383, 435, 509, 991, 1269, 1507
Alw44I GTGCAC 1 cut(s) 762
AlwI GGATC 3 cut(s) 359, 2200, 2645
AlwNI CAGNNNCTG 4 cut(s) 250, 1085, 1304, 2060
AoxI GGCC 9 cut(s) 135, 346, 378, 732, 1035, 1153, 1520, 1759, 2143
ApaLI GTGCAC 1 cut(s) 762
ApoI RAATTY 3 cut(s) 665, 1601, 1828
Asp700I GAANNNNTTC 1 cut(s) 2155
AspLEI GCGC 2 cut(s) 279, 2350
AspS9I GGNCC 5 cut(s) 52, 178, 346, 1760, 1874
AsuHPI GGTGA 2 cut(s) 1158, 2189
AvaII GGWCC 3 cut(s) 52, 178, 1874
BaeGI GKGCMC 2 cut(s) 766, 1282
BalI TGGCCA 2 cut(s) 734, 1037
BanI GGYRCC 3 cut(s) 793, 2313, 2628
BauI CACGAG 2 cut(s) 2073, 2534
BbsI GAAGAC 3 cut(s) 64, 636, 788
Bbv12I GWGCWC 2 cut(s) 766, 1438
BccI CCATC 7 cut(s) 516, 1171, 1177, 1240, 1421, 1845, 2619
BceAI ACGGC 2 cut(s) 1140, 2546
BcgI CGANNNNNNTGC 2 cut(s) 1848, 1882
BciVI GTATCC 1 cut(s) 1250
BclI TGATCA 1 cut(s) 2221
BcoDI GTCTC 7 cut(s) 305, 383, 435, 509, 991, 1269, 1507
BcuI ACTAGT 1 cut(s) 1961
BfuAI ACCTGC 1 cut(s) 592
BfuI GTATCC 1 cut(s) 1250
BglII AGATCT 1 cut(s) 727
Bme18I GGWCC 3 cut(s) 52, 178, 1874
BmgT120I GGNCC 5 cut(s) 52, 178, 346, 1760, 1874
BmiI GGNNCC 6 cut(s) 179, 795, 1616, 2315, 2541, 2630
BmrI ACTGGG 2 cut(s) 975, 1011
BmsI GCATC 8 cut(s) 247, 759, 812, 862, 962, 1171, 1209, 2232
BmuI ACTGGG 2 cut(s) 975, 1011
BpiI GAAGAC 3 cut(s) 64, 636, 788
BplI GAGNNNNNCTC 2 cut(s) 2290, 2322
BpmI CTGGAG 3 cut(s) 5, 1422, 2055
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 8 cut(s) 29, 128, 586, 1392, 1737, 2049, 2267, 2693
BsaBI GATNNNNATC 1 cut(s) 2082
BsaHI GRCGYC 2 cut(s) 63, 69
BsaXI ACNNNNNCTCC 4 cut(s) 413, 443, 904, 934
Bsc4I CCNNNNNNNGG 9 cut(s) 148, 369, 773, 929, 1593, 1670, 1769, 1790, 2132
Bse1I ACTGG 5 cut(s) 204, 863, 970, 1017, 1439
Bse3DI GCAATG 1 cut(s) 1264
Bse8I GATNNNNATC 1 cut(s) 2082
BseGI GGATG 4 cut(s) 262, 1897, 2630, 2683
BseJI GATNNNNATC 1 cut(s) 2082
BseLI CCNNNNNNNGG 9 cut(s) 148, 369, 773, 929, 1593, 1670, 1769, 1790, 2132
BseMI GCAATG 1 cut(s) 1264
BseMII CTCAG 6 cut(s) 444, 446, 581, 588, 2298, 2637
BseNI ACTGG 5 cut(s) 204, 863, 970, 1017, 1439
BseRI GAGGAG 2 cut(s) 1653, 2552
BseSI GKGCMC 2 cut(s) 766, 1282
BsgI GTGCAG 4 cut(s) 1308, 1385, 1646, 1680
Bsh1236I CGCG 2 cut(s) 39, 279
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 9 cut(s) 137, 348, 380, 734, 1037, 1155, 1522, 1761, 2145
BshNI GGYRCC 3 cut(s) 793, 2313, 2628
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 766, 1438
BslFI GGGAC 3 cut(s) 191, 703, 2587
BslI CCNNNNNNNGG 9 cut(s) 148, 369, 773, 929, 1593, 1670, 1769, 1790, 2132
BsmAI GTCTC 7 cut(s) 305, 383, 435, 509, 991, 1269, 1507
BsmFI GGGAC 3 cut(s) 191, 703, 2587
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 9 cut(s) 137, 348, 380, 734, 1037, 1155, 1522, 1761, 2145
Bsp1286I GDGCHC 3 cut(s) 766, 1282, 1438
BspACI CCGC 6 cut(s) 37, 801, 819, 1612, 1952, 2468
BspANI GGCC 9 cut(s) 137, 348, 380, 734, 1037, 1155, 1522, 1761, 2145
BspCNI CTCAG 6 cut(s) 445, 445, 582, 589, 2297, 2638
BspFNI CGCG 2 cut(s) 39, 279
BspLI GGNNCC 6 cut(s) 179, 795, 1616, 2315, 2541, 2630
BspMI ACCTGC 1 cut(s) 592
BspPI GGATC 3 cut(s) 359, 2200, 2645
BspQI GCTCTTC 1 cut(s) 2053
BspT107I GGYRCC 3 cut(s) 793, 2313, 2628
BsrDI GCAATG 1 cut(s) 1264
BsrI ACTGG 5 cut(s) 204, 863, 970, 1017, 1439
BssNI GRCGYC 2 cut(s) 63, 69
BssSI CACGAG 2 cut(s) 2073, 2534
BssT1I CCWWGG 4 cut(s) 1282, 1416, 2131, 2309
Bst2BI CACGAG 2 cut(s) 2073, 2534
Bst4CI ACNGT 7 cut(s) 890, 1643, 1722, 1798, 1845, 2109, 2388
Bst6I CTCTTC 2 cut(s) 1128, 2053
BstACI GRCGYC 2 cut(s) 63, 69
BstAPI GCANNNNNTGC 1 cut(s) 1085
BstC8I GCNNGC 8 cut(s) 246, 262, 1144, 1511, 1658, 1759, 2061, 2352
BstDEI CTNAG 7 cut(s) 432, 453, 590, 597, 2284, 2333, 2646
BstENI CCTNNNNNAGG 2 cut(s) 146, 1767
BstF5I GGATG 4 cut(s) 262, 1897, 2630, 2683
BstFNI CGCG 2 cut(s) 39, 279
BstHHI GCGC 2 cut(s) 279, 2350
BstMAI GTCTC 7 cut(s) 305, 383, 435, 509, 991, 1269, 1507
BstMCI CGRYCG 1 cut(s) 37
BstNSI RCATGY 2 cut(s) 1513, 1660
BstSLI GKGCMC 2 cut(s) 766, 1282
BstUI CGCG 2 cut(s) 39, 279
BstV2I GAAGAC 3 cut(s) 64, 636, 788
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 9 cut(s) 137, 348, 380, 734, 1037, 1155, 1522, 1761, 2145
BtsCI GGATG 4 cut(s) 262, 1897, 2630, 2683
BtsI GCAGTG 2 cut(s) 809, 1520
BtsIMutI CAGTG 6 cut(s) 211, 235, 809, 1024, 1520, 2409
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 8 cut(s) 246, 262, 1144, 1511, 1658, 1759, 2061, 2352
CaiI CAGNNNCTG 4 cut(s) 250, 1085, 1304, 2060
CfoI GCGC 2 cut(s) 279, 2350
Cfr13I GGNCC 5 cut(s) 52, 178, 346, 1760, 1874
CseI GACGC 1 cut(s) 52
Csp6I GTAC 6 cut(s) 172, 1644, 1775, 1794, 2069, 2584
CviAII CATG 4 cut(s) 974, 1510, 1657, 2219
CviQI GTAC 6 cut(s) 172, 1644, 1775, 1794, 2069, 2584
DdeI CTNAG 7 cut(s) 432, 453, 590, 597, 2284, 2333, 2646
EaeI YGGCCR 4 cut(s) 732, 1035, 1153, 2143
Eam1104I CTCTTC 2 cut(s) 1128, 2053
EarI CTCTTC 2 cut(s) 1128, 2053
Eco130I CCWWGG 4 cut(s) 1282, 1416, 2131, 2309
Eco147I AGGCCT 1 cut(s) 1522
Eco47I GGWCC 3 cut(s) 52, 178, 1874
Eco57I CTGAAG 3 cut(s) 149, 1281, 2501
EcoNI CCTNNNNNAGG 2 cut(s) 146, 1767
EcoT14I CCWWGG 4 cut(s) 1282, 1416, 2131, 2309
ErhI CCWWGG 4 cut(s) 1282, 1416, 2131, 2309
FaeI CATG 4 cut(s) 977, 1513, 1660, 2222
FaqI GGGAC 3 cut(s) 191, 703, 2587
FatI CATG 4 cut(s) 973, 1509, 1656, 2218
FauI CCCGC 2 cut(s) 808, 826
FauNDI CATATG 2 cut(s) 2014, 2404
FbaI TGATCA 1 cut(s) 2221
FblI GTMKAC 3 cut(s) 66, 1159, 1946
FokI GGATG 4 cut(s) 269, 1884, 2637, 2690
GlaI GCGC 2 cut(s) 278, 2349
GsuI CTGGAG 3 cut(s) 5, 1422, 2055
HaeIII GGCC 9 cut(s) 137, 348, 380, 734, 1037, 1155, 1522, 1761, 2145
HgaI GACGC 1 cut(s) 52
HhaI GCGC 2 cut(s) 279, 2350
Hin1I GRCGYC 2 cut(s) 63, 69
Hin1II CATG 4 cut(s) 977, 1513, 1660, 2222
Hin6I GCGC 2 cut(s) 277, 2348
HinP1I GCGC 2 cut(s) 277, 2348
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 2 cut(s) 161, 1144
HphI GGTGA 2 cut(s) 1158, 2189
Hpy166II GTNNAC 7 cut(s) 67, 97, 764, 1160, 1947, 2584, 2652
Hpy8I GTNNAC 7 cut(s) 67, 97, 764, 1160, 1947, 2584, 2652
Hpy99I CGWCG 2 cut(s) 68, 71
HpyCH4III ACNGT 7 cut(s) 890, 1643, 1722, 1798, 1845, 2109, 2388
HpyCH4IV ACGT 4 cut(s) 69, 411, 2290, 2576
HpyF3I CTNAG 7 cut(s) 432, 453, 590, 597, 2284, 2333, 2646
HpySE526I ACGT 4 cut(s) 69, 411, 2290, 2576
Hsp92I GRCGYC 2 cut(s) 63, 69
Hsp92II CATG 4 cut(s) 977, 1513, 1660, 2222
HspAI GCGC 2 cut(s) 277, 2348
Ksp22I TGATCA 1 cut(s) 2221
LguI GCTCTTC 1 cut(s) 2053
LmnI GCTCC 6 cut(s) 24, 1331, 1441, 1620, 2298, 2539
LweI GCATC 8 cut(s) 247, 759, 812, 862, 962, 1171, 1209, 2232
MaeII ACGT 4 cut(s) 69, 411, 2290, 2576
MaeIII GTNAC 3 cut(s) 1066, 1309, 1637
MfeI CAATTG 1 cut(s) 2172
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 3 cut(s) 766, 1282, 1438
MlsI TGGCCA 2 cut(s) 734, 1037
MluNI TGGCCA 2 cut(s) 734, 1037
MmeI TCCRAC 4 cut(s) 405, 1025, 1642, 1852
Mox20I TGGCCA 2 cut(s) 734, 1037
MroXI GAANNNNTTC 1 cut(s) 2155
MscI TGGCCA 2 cut(s) 734, 1037
MseI TTAA 5 cut(s) 156, 474, 615, 1725, 2364
Msp20I TGGCCA 2 cut(s) 734, 1037
MspA1I CMGCKG 2 cut(s) 821, 1612
MunI CAATTG 1 cut(s) 2172
Mva1269I GAATGC 1 cut(s) 1081
MvnI CGCG 2 cut(s) 39, 279
NdeI CATATG 2 cut(s) 2014, 2404
NlaIII CATG 4 cut(s) 977, 1513, 1660, 2222
NlaIV GGNNCC 6 cut(s) 179, 795, 1616, 2315, 2541, 2630
NmeAIII GCCGAG 1 cut(s) 508
NmuCI GTSAC 3 cut(s) 1066, 1309, 1637
NspI RCATGY 2 cut(s) 1513, 1660
PaeI GCATGC 2 cut(s) 1513, 1660
PceI AGGCCT 1 cut(s) 1522
PciSI GCTCTTC 1 cut(s) 2053
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 110
PctI GAATGC 1 cut(s) 1081
PdmI GAANNNNTTC 1 cut(s) 2155
PfeI GAWTC 4 cut(s) 196, 1445, 1981, 2024
PflMI CCANNNNNTGG 1 cut(s) 1790
PspN4I GGNNCC 6 cut(s) 179, 795, 1616, 2315, 2541, 2630
PspPI GGNCC 5 cut(s) 52, 178, 346, 1760, 1874
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstNI CAGNNNCTG 4 cut(s) 250, 1085, 1304, 2060
PsuI RGATCY 1 cut(s) 727
RsaI GTAC 6 cut(s) 173, 1645, 1776, 1795, 2070, 2585
RsaNI GTAC 6 cut(s) 172, 1644, 1775, 1794, 2069, 2584
SalI GTCGAC 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 2053
SaqAI TTAA 5 cut(s) 156, 474, 615, 1725, 2364
Sau96I GGNCC 5 cut(s) 52, 178, 346, 1760, 1874
SduI GDGCHC 3 cut(s) 766, 1282, 1438
SfaNI GCATC 8 cut(s) 247, 759, 812, 862, 962, 1171, 1209, 2232
SgrDI CGTCGACG 1 cut(s) 65
SinI GGWCC 3 cut(s) 52, 178, 1874
SmlI CTYRAG 8 cut(s) 44, 143, 565, 1407, 1752, 2064, 2246, 2672
SmoI CTYRAG 8 cut(s) 44, 143, 565, 1407, 1752, 2064, 2246, 2672
SpeI ACTAGT 1 cut(s) 1961
SphI GCATGC 2 cut(s) 1513, 1660
SseBI AGGCCT 1 cut(s) 1522
SsiI CCGC 6 cut(s) 37, 801, 819, 1612, 1952, 2468
SspI AATATT 1 cut(s) 2462
StuI AGGCCT 1 cut(s) 1522
StyI CCWWGG 4 cut(s) 1282, 1416, 2131, 2309
TaaI ACNGT 7 cut(s) 890, 1643, 1722, 1798, 1845, 2109, 2388
TaiI ACGT 4 cut(s) 72, 414, 2293, 2579
TaqI TCGA 4 cut(s) 33, 66, 75, 1207
TatI WGTACW 1 cut(s) 2583
TauI GCSGC 2 cut(s) 1615, 2471
TfiI GAWTC 4 cut(s) 196, 1445, 1981, 2024
Tru1I TTAA 5 cut(s) 156, 474, 615, 1725, 2364
Tru9I TTAA 5 cut(s) 156, 474, 615, 1725, 2364
TscAI CASTG 6 cut(s) 211, 235, 816, 1024, 1520, 2416
TseFI GTSAC 3 cut(s) 1066, 1309, 1637
Tsp45I GTSAC 3 cut(s) 1066, 1309, 1637
TspGWI ACGGA 2 cut(s) 119, 2637
TspRI CASTG 6 cut(s) 211, 235, 816, 1024, 1520, 2416
Van91I CCANNNNNTGG 1 cut(s) 1790
VneI GTGCAC 1 cut(s) 762
VpaK11BI GGWCC 3 cut(s) 52, 178, 1874
XagI CCTNNNNNAGG 2 cut(s) 146, 1767
XapI RAATTY 3 cut(s) 665, 1601, 1828
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 1513, 1660
XmiI GTMKAC 3 cut(s) 66, 1159, 1946
XmnI GAANNNNTTC 1 cut(s) 2155
ZraI GACGTC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.