FvH4_5g18220

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006861
Physical Location & Seq
Forward (+)
10472383 .. 10480486
8104 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18220.t2

Sequence Viewer

Length: 2706 bp
ATGGGGGCGAGTCGGAAGCTCCAGGGCGAGATCGACCGCGTTCTGAAGAAGGTCCAGGAAGGCGTAGATGTCTTCGACAGTATCTGGAACAAGGTTTATGACACGGACAATGCGAACCAGAAGGAGAAGTTTGAGGCCGACTTGAAGAAGGAGATTAAGAAGCTCCAGAGGTACAGGGACCAAATCAAGACTTGGATTCAGTCCAGTGAAATCAAGGATAAGAAGGTGAGTGCGTCGTACGAGTCGGCTCTGGTGGATGCTCGGAAGCAAATTGAGCGTGAAATGGAAAGGTTTAAGATATGCGAAAAGGAGACCAAGACGAAAGCGTTTTCGAAAGAGGGATTGGGCCAGCAACCGAAAACTGATCCGAAGGAGAAGGCTAAATCAGAGACAAGGGATTGGATAAACACTGTGGTTGGGGAGTTGGAATCTCAGATTGACAGCTTTGAAGCTGAGATTGAGGGGGTATCAGTCAAGAAAGGAAAGGGCAGACCGCCTAGACTGACCCATCTAGAGACATCTATCACTCGGCATAAGGCTCATATAATGAAGTTGGAACTGATCTTAAGGTTGTTGGATAATGATGAACTGAGTCCTGAGCAGGTCAATGATGTCAAAGACTTTTTGGAAGACTATGTTGAACGTAATCAGGAGGACTTTGATGAGTTTAGTGAAGTTGATGAGCTTTACAGCACCTTACCATTAGACAAGGTGGAGCTTGAAGATCTGGTTATTGTTCCTCCTGGTCTTGTTAAGGGTGCACCGATGATTGGCTTGAAGACTCCATTGGCAGCATCAGCATCTCAAATGCCTGCCACTGTTACCTCCAGTAACCAGCCGAGTACTCCTGTACAAGAGCCGGTTGAGGATATGGTTTCCCAGGATAATAGTAATCCAGATAACATTGCTAAAACTCCACCTCCAAAAAGCAGTGCACTTGCTTCTGTTGCATCAACACCAACTGGGAATCAAGCAACGCCTGTCTCGTTGAATGTTTCGTCTCTCAGTTTGCCTGGCGTAACAGCTGTTTCAGCTGTTGCCGGTTCAAATGCTGTTCATGGCATCACAGAGAATGCAGGGGCTGCGCTTTCTTCATCTCCTGGAAGTTTGTCTCCTTCTGTGAAGGAAGAAGAATTAGGAACCTTCCCTGGACGTAGACCATCACCATCGCTTTCTGATACTGGAGTTGTGAGAGGCGTTGGTAAAGGTGGCCTCGCTGGCCAGATCCCATCTAGTATACCTCTTAGTTCTAGCAATGTAGTTCCTAGTAATACTGCCCTGGGTGCAGTCCCCTCTGTTACTGACGTGGCAAAGAGAAATATTTTGGGAGCTGATGAGAGACTTGGAGGTAGTGGCGTGGTACAGCCTCTTGTTTCCCCTCTGAGTCACCGGATGATCTTGCCTCAGGCTTCCAAATCCAGTGATGGAAGTGGTCCAGTTGATTCTAGTAATACAAGTGAGGCTACAACTATACCTGGCCGAGCATTTTCTCCGTCTATGGTCTCTGGCATGCAATGGAGGCCAGGAAGTTCCTTCCCAAACCAGAATGAAGCGGGATTGTTTCGTGGAAGAACTGAAATAGCACCTGATCAGAGGGAGAAGTTCTTGCAGCGGCTCCAGCAAGTGCAGCAACAAGGTCACAGTACCATTCATAGCATGCCCGCTCTTGCTGGTGGAAGTTCTAAACAGTTTTCATCACAGCAGCAAAATCCACTCTTGCAACAGTTTAATTCTCAAAGCTCATCTGTACCTTCTCAAGCTGGCATGGGACTTGGGGTACAGGCCCCAGGTCTTGGAACGGTTTCATCCACTACCTTACAGCAGCAGATAAATTCCATCCATCAACAGTCTCCACAGGCTAATCCACAGGCATTGATGTCTAGTGGGCCAAAAGAATCTGATGCTGGTCATCCAAAAGTTGAGGACCAGCAGCAACAGAGTATTCCTGATGACTCAACATCTGAATCCGCTCCTAGTTCTGGGATCGTAAAGAATCTAATGAATGAGGATGATATGAAAGCGTCATATGCAATAGATTCTCTGACTGGAGTTTCTGGCTCTTTGACAGAGTCTTCTCAAGTACCACGAGATATCGATCTGTCTCCTGGGCAACCTTTGCAGTCTAGTCAACCTTCTGCTAGCCTTGGTGTTATTGGCCGAAGAAGTGTTTCTGATCTTGGTACAATTGGCGATAACCTCTCTGGATCAATTGCTAATTCTGGGCCGGGGCACGATCAGTTGTATAATTTGCAGATGCTTGAGGCTGCTTTTTACAAACTGCCACAACCCAAAGACTCTGAACGTGCAAGAAGCTACACTCCAAGGCACCCTGCAATAACTCCTCCTAGCTATCCCCAAGTTCAAGCACCTATAGTTAATAATCCTGCTTTCTGGGAACGTTTGGGTTCTGAACAGTATTCTACTGACACCCTGTTCTTTGCATTTTACTATCAGCAGAACACTTACCAGCAATATTTGGCTGCCAAAGAGTTGAAGAAGCAATCTTGGAGATACCACAGGAAATACAACACTTGGTTTCAACGACATGAAGAGCCAAAAGTGGCCACTGATGAATATGAACAGGGGACATATGTGTACTTTGACTTCCACATTGCCAATGATGATCTACAACATGGATGGTGTCAAAGGATCAAAACTGAGTTTACCTTTGAATACAACTATCTTGAAGATGAACTTATCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

902

Amino Acids

98.29

Weight (kDa)

5.32

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Not3 PF04065 9 - 162 4.9e-46 Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C PF04153 685 - 822 7.6e-34 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 1 cut(s) 2325
AccB7I CCANNNNNTGG 1 cut(s) 1793
AccBSI CCGCTC 2 cut(s) 1664, 1970
AccI GTMKAC 2 cut(s) 1156, 1237
AccII CGCG 1 cut(s) 39
AciI CCGC 6 cut(s) 37, 494, 1553, 1612, 1662, 1968
AclI AACGTT 1 cut(s) 2398
AclWI GGATC 5 cut(s) 359, 1219, 1991, 2212, 2657
AcoI YGGCCR 4 cut(s) 1219, 1477, 2155, 2562
AcsI RAATTY 1 cut(s) 1831
AcuI CTGAAG 1 cut(s) 65
AfiI CCNNNNNNNGG 3 cut(s) 770, 1793, 1979
AflII CTTAAG 1 cut(s) 565
AjiI CACGTC 1 cut(s) 1306
AjuI GAANNNNNNNTTGG 4 cut(s) 326, 358, 770, 802
Alw21I GWGCWC 2 cut(s) 763, 937
Alw44I GTGCAC 2 cut(s) 759, 933
AlwI GGATC 5 cut(s) 359, 1219, 1991, 2212, 2657
AlwNI CAGNNNCTG 2 cut(s) 84, 1082
ApaLI GTGCAC 2 cut(s) 759, 933
ApeKI GCWGC 9 cut(s) 791, 1082, 1609, 1627, 1702, 1822, 1930, 2264, 2480
ApoI RAATTY 1 cut(s) 1831
Asp700I GAANNNNTTC 2 cut(s) 1801, 2167
AspLEI GCGC 1 cut(s) 1087
AspS9I GGNCC 8 cut(s) 52, 178, 346, 1433, 1783, 1886, 1924, 2222
AsuC2I CCSGG 1 cut(s) 2226
AsuHPI GGTGA 3 cut(s) 238, 1155, 1379
AsuII TTCGAA 1 cut(s) 332
AsuNHI GCTAGC 1 cut(s) 2138
AvaII GGWCC 4 cut(s) 52, 178, 1433, 1924
AxyI CCTNAGG 1 cut(s) 1404
BaeGI GKGCMC 3 cut(s) 763, 937, 2232
BaeI ACNNNNGTAYC 2 cut(s) 1170, 1203
BalI TGGCCA 2 cut(s) 1221, 2564
BanI GGYRCC 1 cut(s) 2325
BarI GAAGNNNNNNTAC 2 cut(s) 2588, 2620
BauI CACGAG 1 cut(s) 2085
BbsI GAAGAC 4 cut(s) 64, 636, 785, 2064
Bbv12I GWGCWC 2 cut(s) 763, 937
BbvI GCAGC 9 cut(s) 803, 1069, 1621, 1639, 1714, 1834, 1942, 2251, 2467
BccI CCATC 8 cut(s) 516, 1168, 1174, 1237, 1418, 1844, 1848, 2631
BclI TGATCA 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 2226
BfmI CTRYAG 1 cut(s) 2370
BfrI CTTAAG 1 cut(s) 565
BfuAI ACCTGC 1 cut(s) 592
BglI GCCNNNNNGGC 1 cut(s) 1218
BglII AGATCT 1 cut(s) 724
BmcAI AGTACT 1 cut(s) 844
Bme18I GGWCC 4 cut(s) 52, 178, 1433, 1924
BmgBI CACGTC 1 cut(s) 1306
BmgT120I GGNCC 8 cut(s) 52, 178, 346, 1433, 1783, 1886, 1924, 2222
BmiI GGNNCC 5 cut(s) 179, 1141, 1616, 1785, 2327
BmrI ACTGGG 1 cut(s) 972
BmsI GCATC 7 cut(s) 247, 803, 809, 959, 1071, 1891, 2244
BmtI GCTAGC 1 cut(s) 2142
BmuI ACTGGG 1 cut(s) 972
BpiI GAAGAC 4 cut(s) 64, 636, 785, 2064
BpmI CTGGAG 6 cut(s) 5, 149, 811, 1203, 1601, 2067
Bpu10I CCTNAGC 1 cut(s) 597
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 3 cut(s) 1740, 2061, 2279
BpuMI CCSGG 1 cut(s) 2226
Bsa29I ATCGAT 1 cut(s) 2094
BsaBI GATNNNNATC 1 cut(s) 2094
BsaI GGTCTC 2 cut(s) 305, 1507
BsaWI WCCGGW 1 cut(s) 1389
BsaXI ACNNNNNCTCC 2 cut(s) 904, 934
Bsc4I CCNNNNNNNGG 3 cut(s) 770, 1793, 1979
Bse118I RCCGGY 2 cut(s) 859, 1040
Bse1I ACTGG 7 cut(s) 204, 828, 967, 1186, 1419, 1436, 2050
Bse21I CCTNAGG 1 cut(s) 1404
Bse3DI GCAATG 4 cut(s) 903, 1261, 1520, 2610
Bse8I GATNNNNATC 1 cut(s) 2094
BseCI ATCGAT 1 cut(s) 2094
BseGI GGATG 7 cut(s) 262, 1398, 1805, 1836, 1909, 2014, 2642
BseJI GATNNNNATC 1 cut(s) 2094
BseLI CCNNNNNNNGG 3 cut(s) 770, 1793, 1979
BseMI GCAATG 4 cut(s) 903, 1261, 1520, 2610
BseMII CTCAG 8 cut(s) 444, 446, 581, 588, 1018, 1373, 1418, 2649
BseNI ACTGG 7 cut(s) 204, 828, 967, 1186, 1419, 1436, 2050
BseRI GAGGAG 1 cut(s) 2331
BseSI GKGCMC 3 cut(s) 763, 937, 2232
BseXI GCAGC 9 cut(s) 803, 1069, 1621, 1639, 1714, 1834, 1942, 2251, 2467
BsgI GTGCAG 2 cut(s) 1305, 1646
Bsh1236I CGCG 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 37
BshNI GGYRCC 1 cut(s) 2325
BshVI ATCGAT 1 cut(s) 2094
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 763, 937
BsiSI CCGG 4 cut(s) 860, 1041, 1390, 2225
BsiWI CGTACG 1 cut(s) 237
BslFI GGGAC 4 cut(s) 191, 1274, 1782, 2599
BslI CCNNNNNNNGG 3 cut(s) 770, 1793, 1979
BsmBI CGTCTC 1 cut(s) 1005
BsmFI GGGAC 4 cut(s) 191, 1274, 1782, 2599
BsmI GAATGC 1 cut(s) 1078
Bso31I GGTCTC 2 cut(s) 305, 1507
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 3 cut(s) 763, 937, 2232
Bsp1407I TGTACA 1 cut(s) 850
BspACI CCGC 6 cut(s) 37, 494, 1553, 1612, 1662, 1968
BspCNI CTCAG 8 cut(s) 445, 445, 582, 589, 1017, 1374, 1417, 2650
BspDI ATCGAT 1 cut(s) 2094
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 5 cut(s) 179, 1141, 1616, 1785, 2327
BspMI ACCTGC 1 cut(s) 592
BspOI GCTAGC 1 cut(s) 2142
BspPI GGATC 5 cut(s) 359, 1219, 1991, 2212, 2657
BspQI GCTCTTC 1 cut(s) 2544
BspT104I TTCGAA 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 2325
BspTI CTTAAG 1 cut(s) 565
BspTNI GGTCTC 2 cut(s) 305, 1507
BsrBI CCGCTC 2 cut(s) 1664, 1970
BsrDI GCAATG 4 cut(s) 903, 1261, 1520, 2610
BsrFI RCCGGY 2 cut(s) 859, 1040
BsrGI TGTACA 1 cut(s) 850
BsrI ACTGG 7 cut(s) 204, 828, 967, 1186, 1419, 1436, 2050
BssAI RCCGGY 2 cut(s) 859, 1040
BssNAI GTATAC 1 cut(s) 1238
BssSI CACGAG 1 cut(s) 2085
BssT1I CCWWGG 2 cut(s) 2143, 2321
Bst1107I GTATAC 1 cut(s) 1238
Bst2BI CACGAG 1 cut(s) 2085
Bst4CI ACNGT 9 cut(s) 80, 412, 820, 1643, 1689, 1725, 1801, 1848, 2415
Bst6I CTCTTC 1 cut(s) 2544
BstAFI CTTAAG 1 cut(s) 565
BstAPI GCANNNNNTGC 2 cut(s) 1082, 2116
BstAUI TGTACA 1 cut(s) 850
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 8 cut(s) 350, 813, 1219, 1511, 1658, 1662, 1762, 2140
BstDEI CTNAG 9 cut(s) 432, 453, 590, 597, 1004, 1244, 1382, 1404, 2658
BstF5I GGATG 7 cut(s) 262, 1398, 1805, 1836, 1909, 2014, 2642
BstFNI CGCG 1 cut(s) 39
BstHHI GCGC 1 cut(s) 1087
BstMCI CGRYCG 1 cut(s) 37
BstNSI RCATGY 2 cut(s) 1513, 1660
BstSFI CTRYAG 1 cut(s) 2370
BstSLI GKGCMC 3 cut(s) 763, 937, 2232
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 9 cut(s) 803, 1069, 1621, 1639, 1714, 1834, 1942, 2251, 2467
BstV2I GAAGAC 4 cut(s) 64, 636, 785, 2064
BstX2I RGATCY 2 cut(s) 724, 1224
BstYI RGATCY 2 cut(s) 724, 1224
BstZ17I GTATAC 1 cut(s) 1238
Bsu15I ATCGAT 1 cut(s) 2094
Bsu36I CCTNAGG 1 cut(s) 1404
BsuTUI ATCGAT 1 cut(s) 2094
BtgZI GCGATG 1 cut(s) 1152
BtrI CACGTC 1 cut(s) 1306
BtsCI GGATG 7 cut(s) 262, 1398, 1805, 1836, 1909, 2014, 2642
BtsI GCAGTG 1 cut(s) 937
BtsIMutI CAGTG 6 cut(s) 211, 408, 816, 937, 1426, 2565
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 8 cut(s) 350, 813, 1219, 1511, 1658, 1662, 1762, 2140
CaiI CAGNNNCTG 2 cut(s) 84, 1082
CfoI GCGC 1 cut(s) 1087
Cfr10I RCCGGY 2 cut(s) 859, 1040
Cfr13I GGNCC 8 cut(s) 52, 178, 346, 1433, 1783, 1886, 1924, 2222
ClaI ATCGAT 1 cut(s) 2094
CseI GACGC 2 cut(s) 222, 2010
CspCI CAANNNNNGTGG 2 cut(s) 1854, 1889
CviAII CATG 6 cut(s) 1058, 1510, 1657, 1765, 2546, 2633
DdeI CTNAG 9 cut(s) 432, 453, 590, 597, 1004, 1244, 1382, 1404, 2658
EaeI YGGCCR 4 cut(s) 1219, 1477, 2155, 2562
Eam1104I CTCTTC 1 cut(s) 2544
EarI CTCTTC 1 cut(s) 2544
Eco130I CCWWGG 2 cut(s) 2143, 2321
Eco31I GGTCTC 2 cut(s) 305, 1507
Eco32I GATATC 1 cut(s) 2092
Eco47I GGWCC 4 cut(s) 52, 178, 1433, 1924
Eco57I CTGAAG 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 1404
EcoO109I RGGNCCY 1 cut(s) 1783
EcoRV GATATC 1 cut(s) 2092
EcoT14I CCWWGG 2 cut(s) 2143, 2321
ErhI CCWWGG 2 cut(s) 2143, 2321
Esp3I CGTCTC 1 cut(s) 1005
FaeI CATG 6 cut(s) 1061, 1513, 1660, 1768, 2549, 2636
FalI AAGNNNNNCTT 1 cut(s) 2679
FaqI GGGAC 4 cut(s) 191, 1274, 1782, 2599
FatI CATG 6 cut(s) 1057, 1509, 1656, 1764, 2545, 2632
FauI CCCGC 2 cut(s) 1546, 1669
FauNDI CATATG 2 cut(s) 2026, 2590
FbaI TGATCA 1 cut(s) 1588
FblI GTMKAC 2 cut(s) 1156, 1237
FokI GGATG 7 cut(s) 269, 1405, 1792, 1823, 1896, 2021, 2649
GlaI GCGC 1 cut(s) 1086
GsuI CTGGAG 6 cut(s) 5, 149, 811, 1203, 1601, 2067
HapII CCGG 4 cut(s) 860, 1041, 1390, 2225
HgaI GACGC 2 cut(s) 222, 2010
HhaI GCGC 1 cut(s) 1087
Hin1II CATG 6 cut(s) 1061, 1513, 1660, 1768, 2549, 2636
Hin6I GCGC 1 cut(s) 1085
HinP1I GCGC 1 cut(s) 1085
HincII GTYRAC 1 cut(s) 2129
HindII GTYRAC 1 cut(s) 2129
HpaII CCGG 4 cut(s) 860, 1041, 1390, 2225
HphI GGTGA 3 cut(s) 238, 1155, 1379
Hpy166II GTNNAC 7 cut(s) 761, 935, 1157, 1238, 2129, 2596, 2664
Hpy8I GTNNAC 7 cut(s) 761, 935, 1157, 1238, 2129, 2596, 2664
Hpy99I CGWCG 1 cut(s) 238
HpyCH4III ACNGT 9 cut(s) 80, 412, 820, 1643, 1689, 1725, 1801, 1848, 2415
HpyCH4IV ACGT 5 cut(s) 643, 1153, 1305, 2302, 2398
HpyF3I CTNAG 9 cut(s) 432, 453, 590, 597, 1004, 1244, 1382, 1404, 2658
HpySE526I ACGT 5 cut(s) 643, 1153, 1305, 2302, 2398
Hsp92II CATG 6 cut(s) 1061, 1513, 1660, 1768, 2549, 2636
HspAI GCGC 1 cut(s) 1085
Ksp22I TGATCA 1 cut(s) 1588
LguI GCTCTTC 1 cut(s) 2544
LmnI GCTCC 6 cut(s) 24, 168, 715, 1328, 1620, 1975
Lsp1109I GCAGC 9 cut(s) 803, 1069, 1621, 1639, 1714, 1834, 1942, 2251, 2467
LweI GCATC 7 cut(s) 247, 803, 809, 959, 1071, 1891, 2244
MaeII ACGT 5 cut(s) 643, 1153, 1305, 2302, 2398
MaeIII GTNAC 6 cut(s) 820, 830, 1018, 1297, 1385, 1637
MbiI CCGCTC 2 cut(s) 1664, 1970
MfeI CAATTG 2 cut(s) 2184, 2208
MflI RGATCY 2 cut(s) 724, 1224
MhlI GDGCHC 3 cut(s) 763, 937, 2232
MlsI TGGCCA 2 cut(s) 1221, 2564
MluCI AATT 8 cut(s) 270, 1133, 1729, 1831, 2184, 2208, 2215, 2245
MluNI TGGCCA 2 cut(s) 1221, 2564
MlyI GAGTC 8 cut(s) 19, 251, 601, 775, 1393, 1946, 2078, 2288
MmeI TCCRAC 3 cut(s) 405, 534, 555
Mox20I TGGCCA 2 cut(s) 1221, 2564
MroXI GAANNNNTTC 2 cut(s) 1801, 2167
MscI TGGCCA 2 cut(s) 1221, 2564
MseI TTAA 6 cut(s) 156, 294, 566, 753, 1728, 2376
Msp20I TGGCCA 2 cut(s) 1221, 2564
MspA1I CMGCKG 3 cut(s) 1025, 1034, 1612
MspCI CTTAAG 1 cut(s) 565
MspI CCGG 4 cut(s) 860, 1041, 1390, 2225
MunI CAATTG 2 cut(s) 2184, 2208
Mva1269I GAATGC 1 cut(s) 1078
MvnI CGCG 1 cut(s) 39
NciI CCSGG 1 cut(s) 2226
NdeI CATATG 2 cut(s) 2026, 2590
NheI GCTAGC 1 cut(s) 2138
NlaIII CATG 6 cut(s) 1061, 1513, 1660, 1768, 2549, 2636
NlaIV GGNNCC 5 cut(s) 179, 1141, 1616, 1785, 2327
NmeAIII GCCGAG 3 cut(s) 508, 864, 1505
NmuCI GTSAC 2 cut(s) 1385, 1637
NspI RCATGY 2 cut(s) 1513, 1660
NspV TTCGAA 1 cut(s) 332
PaeI GCATGC 2 cut(s) 1513, 1660
PasI CCCWGGG 1 cut(s) 1279
PciSI GCTCTTC 1 cut(s) 2544
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 983
PctI GAATGC 1 cut(s) 1078
PdmI GAANNNNTTC 2 cut(s) 1801, 2167
PfeI GAWTC 8 cut(s) 196, 428, 967, 1442, 1895, 1964, 1993, 2036
Pfl23II CGTACG 1 cut(s) 237
PflFI GACNNNGTC 1 cut(s) 2068
PflMI CCANNNNNTGG 1 cut(s) 1793
PfoI TCCNGGA 2 cut(s) 54, 1099
PleI GAGTC 8 cut(s) 18, 250, 600, 775, 1392, 1946, 2077, 2288
PpsI GAGTC 8 cut(s) 18, 250, 600, 775, 1392, 1946, 2077, 2288
Psp1406I AACGTT 1 cut(s) 2398
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 5 cut(s) 179, 1141, 1616, 1785, 2327
PspPI GGNCC 8 cut(s) 52, 178, 346, 1433, 1783, 1886, 1924, 2222
PstNI CAGNNNCTG 2 cut(s) 84, 1082
PsuI RGATCY 2 cut(s) 724, 1224
PsyI GACNNNGTC 1 cut(s) 2068
PvuII CAGCTG 2 cut(s) 1025, 1034
SapI GCTCTTC 1 cut(s) 2544
SaqAI TTAA 6 cut(s) 156, 294, 566, 753, 1728, 2376
Sau96I GGNCC 8 cut(s) 52, 178, 346, 1433, 1783, 1886, 1924, 2222
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 8 cut(s) 19, 251, 601, 775, 1393, 1946, 2078, 2288
SduI GDGCHC 3 cut(s) 763, 937, 2232
SfaNI GCATC 7 cut(s) 247, 803, 809, 959, 1071, 1891, 2244
SfcI CTRYAG 1 cut(s) 2370
SfiI GGCCNNNNNGGCC 1 cut(s) 1218
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 4 cut(s) 52, 178, 1433, 1924
SmlI CTYRAG 4 cut(s) 565, 1755, 2076, 2258
SmoI CTYRAG 4 cut(s) 565, 1755, 2076, 2258
SphI GCATGC 2 cut(s) 1513, 1660
Sse9I AATT 8 cut(s) 270, 1133, 1729, 1831, 2184, 2208, 2215, 2245
SsiI CCGC 6 cut(s) 37, 494, 1553, 1612, 1662, 1968
SspI AATATT 2 cut(s) 1321, 2474
StyI CCWWGG 2 cut(s) 2143, 2321
TaaI ACNGT 9 cut(s) 80, 412, 820, 1643, 1689, 1725, 1801, 1848, 2415
TaiI ACGT 5 cut(s) 646, 1156, 1308, 2305, 2401
TaqI TCGA 4 cut(s) 33, 75, 332, 2094
TasI AATT 8 cut(s) 270, 1133, 1729, 1831, 2184, 2208, 2215, 2245
TatI WGTACW 3 cut(s) 842, 850, 2595
TauI GCSGC 1 cut(s) 1615
TfiI GAWTC 8 cut(s) 196, 428, 967, 1442, 1895, 1964, 1993, 2036
Tru1I TTAA 6 cut(s) 156, 294, 566, 753, 1728, 2376
Tru9I TTAA 6 cut(s) 156, 294, 566, 753, 1728, 2376
TscAI CASTG 6 cut(s) 211, 415, 823, 937, 1426, 2572
TseFI GTSAC 2 cut(s) 1385, 1637
TseI GCWGC 9 cut(s) 791, 1082, 1609, 1627, 1702, 1822, 1930, 2264, 2480
Tsp45I GTSAC 2 cut(s) 1385, 1637
TspGWI ACGGA 2 cut(s) 119, 1482
TspRI CASTG 6 cut(s) 211, 415, 823, 937, 1426, 2572
Tth111I GACNNNGTC 1 cut(s) 2068
Van91I CCANNNNNTGG 1 cut(s) 1793
Vha464I CTTAAG 1 cut(s) 565
VneI GTGCAC 2 cut(s) 759, 933
VpaK11BI GGWCC 4 cut(s) 52, 178, 1433, 1924
XapI RAATTY 1 cut(s) 1831
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 1513, 1660
XmiI GTMKAC 2 cut(s) 1156, 1237
XmnI GAANNNNTTC 2 cut(s) 1801, 2167
ZrmI AGTACT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.