Rroxscaffold_3G00275910

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006861
Physical Location & Seq
Forward (+)
67210957 .. 67218500
7544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275910.1

Sequence Viewer

Length: 2703 bp
ATGGGGGCCAGTCGGAAGCTCCAGGGCGAGATCGACCGGGTTCTCAAGAAGGTCCAAGAAGGCGTGGATGTGTTCGACAGCATTTGGAATAAGGTTTATGACACAGACAACGCGAACCAGAAGGAGAAGTTTGAGGCCGACTTGAAGAAGGAGATTAAGAAGCTCCAGCGGTACAGGGACCAAATCAAGACTTGGATTCAGTCCAGTGAAATCAAGGATAAGAAGGTGAGTGCCTCATACGAGCAGGCTCTGGTGGATGCTCGGAAGCTTATTGAGCGTGAAATGGAAAGGTTTAAGATATGCGAAAAGGAGACCAAAACCAAAGCTTTTTCTAAAGAGGGATTGGGCCAACAACCCAAAACAGATCCCAAGGAGAAGGCTAAATCAGAGACAAGGGATTGGATAAACACTGTGGTAGGGGAGTTGGAATCTCAGATTGACAGCTTTGAAGCTGAGATTGAGGGGGTATCGGTCAAGAAAGGAAAGGGCAGACCGCCTAGACTGACCCATCTAGAGACATCTATTACCCGGCATAAGGCTCATATAATGAAGTTGGAACTGATCTTAAGGTTATTGGATAATGATGAACTCAGTCCTGAGCAGGTCAATGATGTCAAAGACTTCTTGGAAGACTATGTTGAACGTAATCAGGAGGACTTTGATGAGTTTAGTGAAGTTGATGAGCTTTACAGCACCTTACCATTGGACAAGGTGGAGTCCCTTGAAGATATTGTTCCTCCTGGTCTTGTCAAGGGTGCACCGATGCTTGGCTTGAAGACTTCTTTGGCAGCATCAGCATCTCAAATGCCTCCCATTGTTATTACCACTAACCAACCAAGTACTCCTGTACAAGAGCCGGTTGAGGATACAGTTTCCCAGGATAACAGTAATCCTGATAACGTTGCTAGAACTCCACCTCCTAAAAGCAGTGCACTTGGTTCTGCTGCATCAACACCAACTGGGAATCATGCAACTCCTGTCTCTTTGAATGTTTCATCTCTCAGTTTGCCTGGTGTACCAACTGTTTCAGCTGTTGCTGTTTCAAATGCTGTTCGTGGTGTCACAGAGAATGCAGGGGCTGCTCTTTCTTCATCTCCTGTAAGTCTGTCTCCTTCTGTGAAGGAAGAAGAAGTAGCAAGCTTCCCTGGACGTAGATCATCACCATCGCTTTCTGATACTGGAGTTGTGAGAGGCATTGGTAGAGGTGGCCTCACTGGCCAGATCCCATCTAGCATACCTCTTAGTTCTAGCACTGTGGTTCCTAGTAATAATGCCCTAGGTGCAGTCCCCTCTGCTACTGATGTAGCAAAGAGAAATATTTTGGGAGCTGATGAGAGACTTGGAAGTAGTGGTGTGGTACAGTCTCTTGTTTCCCCTCTGAGTAACCGGATGATCTTGCCTCAGGCTTCCAAACCCAGTGATGGAACTGGGCCAGTTGATTCTAGTAATACAAGTGAGGCTACTGCTATACCTGGCCGAACATTTTCCCCTTCTATGGTCTCTGGCATGCAATGGAGGCCCGGAAGTTCCTTCCCAAACCAGAATGAAGCGGGATTGTTTCGTGGAAGAACGGAAATAGCACCTGATCAGAGGGAGAAGTTCTTGCAGCGGCTCCAGCAAGTGCAGCAACAAGGTCACAGTACCATTCTTAGCATGCCCACTCTTTCTGGTGGAAATTCTAAGCAGTTTTCTACTCAGCAGCAGAATCCACTCTTGCAACAGTTTAATTCTCAAAGCTCATCTGTACCTTCTCAAGCTGGCATGGGACTTGGGTTACAGGCACCAGGTCTTGGGACTGTTTCATCTACTACCTTACAGCAGCAGCTAAATTCCATCCATCAACAGTCTCCACAGGCTAATCCACAGGCATTGATGTCAAGTGGACCAAAAGAAGCTGATGCTGGTCATCCAAAAGTTGAGGACCAGCAGCAACAGAATCTTCCTGATGACTCAGCATCTGAATCTGCTCCTAGTTCTGGGCTCGTAAAGAATCTAATGAATGAGGATGATATGAAAGCCTCATATGCAATTGATTCTCTGACTGGTGTTTCTAGCTCTTTGACAGAGTCTTCTCAAGTACCACGAGATATCGATCTCTCTCCTGGGCAACCTTTGCAGTCGAGTCAACCTTCTGCTAGCCTTGGTGTTATTGGCCGAAGAAGTGTTTCTGATCTTGGTGCAATTGGCGATAACCTCTCCGGATCAACTGCTAATTCTGGGGCAATGCATGATCAGTTGTATAATTTGCAGATGCTTGAGGCTGCTTATTACAAACTTCCACAACCCAAAGACTCTGAACGTGCAAGAAGCTACACTCCAAGGCACCCAGCAATAACTCCTCCTAGCTATCCCCAAGTCCAAGCACCTATAGTTAATAATCCTGCTTTCTGGGAACGTTTGGGTTCTGAACAGTATTCTACTGATACCCTGTTCTTTGCATTTTACTATCAGCAGAACACTTACCAGCAATATTTGGCTGCCAAAGAGTTGAAGAAGCAATCTTGGAGATACCACAGGAAATACAACACTTGGTTTCAACGACACGAAGAGCCAAAAGTGGCTACTGATGAATATGAACAGGGTACATATGTGTACTTTGACTTCCATATTGCCAATGATGATCTACAACATGGATGGTGTCAAAGGATCAAAACCGAGTTTACCTTTGAATATAACTATCTTGAAGATGAACTTATCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

900

Amino Acids

98.48

Weight (kDa)

5.3

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Not3 PF04065 4 - 235 2.4e-83 Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C PF04153 756 - 894 9.8e-34 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 2 cut(s) 1780, 2322
AccB7I CCANNNNNTGG 1 cut(s) 1790
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 2198
AciI CCGC 4 cut(s) 169, 494, 1550, 1609
AclI AACGTT 2 cut(s) 900, 2395
AclWI GGATC 4 cut(s) 359, 1216, 2209, 2654
AcoI YGGCCR 3 cut(s) 1216, 1474, 2152
AcsI RAATTY 2 cut(s) 1675, 1828
AfiI CCNNNNNNNGG 6 cut(s) 535, 767, 1421, 1495, 1790, 1976
AflII CTTAAG 1 cut(s) 565
AjnI CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1471, 1783, 2101
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AloI GAACNNNNNNTCC 2 cut(s) 901, 933
Alw21I GWGCWC 2 cut(s) 760, 934
Alw26I GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1851
Alw44I GTGCAC 2 cut(s) 756, 930
AlwI GGATC 4 cut(s) 359, 1216, 2209, 2654
AlwNI CAGNNNCTG 3 cut(s) 250, 1079, 1958
Aor13HI TCCGGA 1 cut(s) 2198
AoxI GGCC 9 cut(s) 6, 135, 346, 1207, 1216, 1430, 1474, 1517, 2152
ApaLI GTGCAC 2 cut(s) 756, 930
ApoI RAATTY 2 cut(s) 1675, 1828
Asp700I GAANNNNTTC 2 cut(s) 1483, 2164
AspA2I CCTAGG 1 cut(s) 1276
AspS9I GGNCC 8 cut(s) 6, 52, 178, 346, 1430, 1518, 1883, 1921
AsuC2I CCSGG 3 cut(s) 38, 529, 1521
AsuHPI GGTGA 2 cut(s) 238, 1152
AsuNHI GCTAGC 1 cut(s) 2135
AvaII GGWCC 4 cut(s) 52, 178, 1883, 1921
AvrII CCTAGG 1 cut(s) 1276
AxyI CCTNAGG 1 cut(s) 1401
BaeGI GKGCMC 2 cut(s) 760, 934
BaeI ACNNNNGTAYC 4 cut(s) 1167, 1200, 2574, 2607
BalI TGGCCA 1 cut(s) 1218
BanI GGYRCC 2 cut(s) 1780, 2322
BanII GRGCYC 1 cut(s) 1983
BarI GAAGNNNNNNTAC 2 cut(s) 2585, 2617
BauI CACGAG 1 cut(s) 2082
BbsI GAAGAC 3 cut(s) 636, 782, 2061
Bbv12I GWGCWC 2 cut(s) 760, 934
BccI CCATC 7 cut(s) 516, 1171, 1234, 1415, 1841, 1845, 2628
BciT130I CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1473, 1785, 2103
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 2 cut(s) 1585, 2230
BcnI CCSGG 3 cut(s) 38, 529, 1521
BcoDI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1851
BfmI CTRYAG 1 cut(s) 2367
BfrI CTTAAG 1 cut(s) 565
BfuAI ACCTGC 1 cut(s) 592
BfuI GTATCC 1 cut(s) 859
BglI GCCNNNNNGGC 1 cut(s) 1215
BlnI CCTAGG 1 cut(s) 1276
BmcAI AGTACT 1 cut(s) 841
Bme18I GGWCC 4 cut(s) 52, 178, 1883, 1921
BmgT120I GGNCC 8 cut(s) 6, 52, 178, 346, 1430, 1518, 1883, 1921
BmiI GGNNCC 6 cut(s) 7, 179, 1260, 1613, 1782, 2324
BmrI ACTGGG 3 cut(s) 969, 1410, 1437
BmsI GCATC 8 cut(s) 247, 753, 800, 806, 956, 1888, 1964, 2241
BmtI GCTAGC 1 cut(s) 2139
BmuI ACTGGG 3 cut(s) 969, 1410, 1437
BpiI GAAGAC 3 cut(s) 636, 782, 2061
BplI GAGNNNNNCTC 2 cut(s) 1194, 1226
BpmI CTGGAG 4 cut(s) 5, 149, 1200, 1598
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 4 cut(s) 29, 1737, 2058, 2276
BpuMI CCSGG 3 cut(s) 38, 529, 1521
Bsa29I ATCGAT 1 cut(s) 2091
BsaBI GATNNNNATC 1 cut(s) 2091
BsaI GGTCTC 2 cut(s) 305, 1504
BsaJI CCNNGG 8 cut(s) 22, 369, 876, 1144, 1276, 2102, 2140, 2318
BsaWI WCCGGW 2 cut(s) 1386, 2198
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bsc4I CCNNNNNNNGG 6 cut(s) 535, 767, 1421, 1495, 1790, 1976
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 9 cut(s) 9, 204, 964, 1183, 1219, 1416, 1432, 1433, 2047
Bse21I CCTNAGG 1 cut(s) 1401
Bse3DI GCAATG 2 cut(s) 1517, 2229
Bse8I GATNNNNATC 1 cut(s) 2091
BseAI TCCGGA 1 cut(s) 2198
BseBI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1473, 1785, 2103
BseCI ATCGAT 1 cut(s) 2091
BseDI CCNNGG 8 cut(s) 22, 369, 876, 1144, 1276, 2102, 2140, 2318
BseGI GGATG 7 cut(s) 73, 262, 1395, 1833, 1906, 2011, 2639
BseJI GATNNNNATC 1 cut(s) 2091
BseLI CCNNNNNNNGG 6 cut(s) 535, 767, 1421, 1495, 1790, 1976
BseMI GCAATG 2 cut(s) 1517, 2229
BseMII CTCAG 9 cut(s) 444, 446, 588, 604, 1015, 1370, 1415, 1709, 1965
BseNI ACTGG 9 cut(s) 9, 204, 964, 1183, 1219, 1416, 1432, 1433, 2047
BseRI GAGGAG 1 cut(s) 2328
BseSI GKGCMC 2 cut(s) 760, 934
BseYI CCCAGC 1 cut(s) 2326
BsgI GTGCAG 2 cut(s) 1302, 1643
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 9 cut(s) 8, 137, 348, 1209, 1218, 1432, 1476, 1519, 2154
BshNI GGYRCC 2 cut(s) 1780, 2322
BshVI ATCGAT 1 cut(s) 2091
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 760, 934
BsiSI CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2199
BslFI GGGAC 5 cut(s) 191, 703, 1271, 1779, 1807
BslI CCNNNNNNNGG 6 cut(s) 535, 767, 1421, 1495, 1790, 1976
BsmAI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1851
BsmFI GGGAC 5 cut(s) 191, 703, 1271, 1779, 1807
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 9 cut(s) 8, 137, 348, 1209, 1218, 1432, 1476, 1519, 2154
Bso31I GGTCTC 2 cut(s) 305, 1504
Bsp1286I GDGCHC 3 cut(s) 760, 934, 1983
Bsp13I TCCGGA 1 cut(s) 2198
Bsp1407I TGTACA 1 cut(s) 847
BspACI CCGC 4 cut(s) 169, 494, 1550, 1609
BspANI GGCC 9 cut(s) 8, 137, 348, 1209, 1218, 1432, 1476, 1519, 2154
BspCNI CTCAG 9 cut(s) 445, 445, 589, 603, 1014, 1371, 1414, 1708, 1964
BspDI ATCGAT 1 cut(s) 2091
BspEI TCCGGA 1 cut(s) 2198
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 6 cut(s) 7, 179, 1260, 1613, 1782, 2324
BspMI ACCTGC 1 cut(s) 592
BspOI GCTAGC 1 cut(s) 2139
BspPI GGATC 4 cut(s) 359, 1216, 2209, 2654
BspQI GCTCTTC 1 cut(s) 2541
BspT107I GGYRCC 2 cut(s) 1780, 2322
BspTI CTTAAG 1 cut(s) 565
BspTNI GGTCTC 2 cut(s) 305, 1504
BsrDI GCAATG 2 cut(s) 1517, 2229
BsrFI RCCGGY 1 cut(s) 856
BsrGI TGTACA 1 cut(s) 847
BsrI ACTGG 9 cut(s) 9, 204, 964, 1183, 1219, 1416, 1432, 1433, 2047
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 8 cut(s) 22, 369, 876, 1144, 1276, 2102, 2140, 2318
BssSI CACGAG 1 cut(s) 2082
BssT1I CCWWGG 4 cut(s) 369, 1276, 2140, 2318
Bst2BI CACGAG 1 cut(s) 2082
Bst2UI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1473, 1785, 2103
Bst6I CTCTTC 1 cut(s) 2541
BstAFI CTTAAG 1 cut(s) 565
BstAPI GCANNNNNTGC 2 cut(s) 1079, 2113
BstAUI TGTACA 1 cut(s) 847
BstC8I GCNNGC 6 cut(s) 246, 1138, 1508, 1655, 1759, 2137
BstF5I GGATG 7 cut(s) 73, 262, 1395, 1833, 1906, 2011, 2639
BstFNI CGCG 1 cut(s) 113
BstMAI GTCTC 9 cut(s) 305, 383, 509, 985, 1113, 1330, 1368, 1504, 1851
BstMCI CGRYCG 1 cut(s) 37
BstNI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1473, 1785, 2103
BstNSI RCATGY 2 cut(s) 1510, 1657
BstSFI CTRYAG 1 cut(s) 2367
BstSLI GKGCMC 2 cut(s) 760, 934
BstUI CGCG 1 cut(s) 113
BstV2I GAAGAC 3 cut(s) 636, 782, 2061
BstX2I RGATCY 2 cut(s) 364, 1221
BstYI RGATCY 2 cut(s) 364, 1221
Bsu15I ATCGAT 1 cut(s) 2091
Bsu36I CCTNAGG 1 cut(s) 1401
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 9 cut(s) 8, 137, 348, 1209, 1218, 1432, 1476, 1519, 2154
BsuTUI ATCGAT 1 cut(s) 2091
BtgZI GCGATG 1 cut(s) 1149
BtsCI GGATG 7 cut(s) 73, 262, 1395, 1833, 1906, 2011, 2639
BtsI GCAGTG 1 cut(s) 934
BtsIMutI CAGTG 6 cut(s) 211, 408, 934, 1212, 1251, 1423
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 6 cut(s) 246, 1138, 1508, 1655, 1759, 2137
CaiI CAGNNNCTG 3 cut(s) 250, 1079, 1958
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 8 cut(s) 6, 52, 178, 346, 1430, 1518, 1883, 1921
ClaI ATCGAT 1 cut(s) 2091
CsiI ACCWGGT 1 cut(s) 1783
CspCI CAANNNNNGTGG 2 cut(s) 1851, 1886
CviAII CATG 6 cut(s) 968, 1507, 1654, 1762, 2228, 2630
EaeI YGGCCR 3 cut(s) 1216, 1474, 2152
Eam1104I CTCTTC 1 cut(s) 2541
EarI CTCTTC 1 cut(s) 2541
Eco130I CCWWGG 4 cut(s) 369, 1276, 2140, 2318
Eco24I GRGCYC 1 cut(s) 1983
Eco31I GGTCTC 2 cut(s) 305, 1504
Eco32I GATATC 1 cut(s) 2089
Eco47I GGWCC 4 cut(s) 52, 178, 1883, 1921
Eco81I CCTNAGG 1 cut(s) 1401
EcoRII CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1471, 1783, 2101
EcoRV GATATC 1 cut(s) 2089
EcoT14I CCWWGG 4 cut(s) 369, 1276, 2140, 2318
EcoT22I ATGCAT 1 cut(s) 2229
EcoT38I GRGCYC 1 cut(s) 1983
ErhI CCWWGG 4 cut(s) 369, 1276, 2140, 2318
FaeI CATG 6 cut(s) 971, 1510, 1657, 1765, 2231, 2633
FalI AAGNNNNNCTT 1 cut(s) 2676
FaqI GGGAC 5 cut(s) 191, 703, 1271, 1779, 1807
FatI CATG 6 cut(s) 967, 1506, 1653, 1761, 2227, 2629
FauI CCCGC 1 cut(s) 1543
FauNDI CATATG 2 cut(s) 2023, 2587
FbaI TGATCA 2 cut(s) 1585, 2230
FokI GGATG 7 cut(s) 80, 269, 1402, 1820, 1893, 2018, 2646
FriOI GRGCYC 1 cut(s) 1983
GsaI CCCAGC 1 cut(s) 2330
GsuI CTGGAG 4 cut(s) 5, 149, 1200, 1598
HaeIII GGCC 9 cut(s) 8, 137, 348, 1209, 1218, 1432, 1476, 1519, 2154
HapII CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2199
Hin1II CATG 6 cut(s) 971, 1510, 1657, 1765, 2231, 2633
HincII GTYRAC 1 cut(s) 2126
HindII GTYRAC 1 cut(s) 2126
HindIII AAGCTT 3 cut(s) 266, 324, 1138
HpaII CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2199
HphI GGTGA 2 cut(s) 238, 1152
Hpy166II GTNNAC 7 cut(s) 758, 932, 1016, 1883, 2126, 2593, 2661
Hpy8I GTNNAC 7 cut(s) 758, 932, 1016, 1883, 2126, 2593, 2661
HpyCH4IV ACGT 5 cut(s) 643, 900, 1150, 2299, 2395
HpySE526I ACGT 5 cut(s) 643, 900, 1150, 2299, 2395
Hsp92II CATG 6 cut(s) 971, 1510, 1657, 1765, 2231, 2633
Kpn2I TCCGGA 1 cut(s) 2198
Ksp22I TGATCA 2 cut(s) 1585, 2230
LguI GCTCTTC 1 cut(s) 2541
LmnI GCTCC 5 cut(s) 24, 168, 1325, 1617, 1972
LweI GCATC 8 cut(s) 247, 753, 800, 806, 956, 1888, 1964, 2241
MabI ACCWGGT 1 cut(s) 1783
MaeII ACGT 5 cut(s) 643, 900, 1150, 2299, 2395
MaeIII GTNAC 4 cut(s) 1060, 1382, 1634, 1773
MfeI CAATTG 2 cut(s) 2028, 2181
MflI RGATCY 2 cut(s) 364, 1221
MhlI GDGCHC 3 cut(s) 760, 934, 1983
MlsI TGGCCA 1 cut(s) 1218
MluCI AATT 7 cut(s) 1675, 1726, 1828, 2028, 2181, 2212, 2242
MluNI TGGCCA 1 cut(s) 1218
MlyI GAGTC 5 cut(s) 725, 1943, 2075, 2131, 2285
MmeI TCCRAC 2 cut(s) 405, 534
Mox20I TGGCCA 1 cut(s) 1218
Mph1103I ATGCAT 1 cut(s) 2229
MroI TCCGGA 1 cut(s) 2198
MroXI GAANNNNTTC 2 cut(s) 1483, 2164
MscI TGGCCA 1 cut(s) 1218
MseI TTAA 5 cut(s) 156, 294, 566, 1725, 2373
Msp20I TGGCCA 1 cut(s) 1218
MspA1I CMGCKG 3 cut(s) 169, 1031, 1609
MspCI CTTAAG 1 cut(s) 565
MspI CCGG 6 cut(s) 37, 529, 857, 1387, 1521, 2199
MunI CAATTG 2 cut(s) 2028, 2181
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 8 cut(s) 23, 741, 878, 1011, 1146, 1473, 1785, 2103
MvnI CGCG 1 cut(s) 113
NciI CCSGG 3 cut(s) 38, 529, 1521
NdeI CATATG 2 cut(s) 2023, 2587
NheI GCTAGC 1 cut(s) 2135
NlaIII CATG 6 cut(s) 971, 1510, 1657, 1765, 2231, 2633
NlaIV GGNNCC 6 cut(s) 7, 179, 1260, 1613, 1782, 2324
NmuCI GTSAC 2 cut(s) 1060, 1634
NsiI ATGCAT 1 cut(s) 2229
NspI RCATGY 2 cut(s) 1510, 1657
PaeI GCATGC 2 cut(s) 1510, 1657
PciSI GCTCTTC 1 cut(s) 2541
PctI GAATGC 1 cut(s) 1075
PdmI GAANNNNTTC 2 cut(s) 1483, 2164
PfeI GAWTC 9 cut(s) 196, 428, 964, 1439, 1705, 1936, 1961, 1990, 2033
PflFI GACNNNGTC 1 cut(s) 2065
PflMI CCANNNNNTGG 1 cut(s) 1790
PleI GAGTC 5 cut(s) 724, 1943, 2074, 2130, 2285
PpsI GAGTC 5 cut(s) 724, 1943, 2074, 2130, 2285
Psp1406I AACGTT 2 cut(s) 900, 2395
Psp6I CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1471, 1783, 2101
PspFI CCCAGC 1 cut(s) 2326
PspGI CCWGG 8 cut(s) 21, 739, 876, 1009, 1144, 1471, 1783, 2101
PspN4I GGNNCC 6 cut(s) 7, 179, 1260, 1613, 1782, 2324
PspPI GGNCC 8 cut(s) 6, 52, 178, 346, 1430, 1518, 1883, 1921
PstNI CAGNNNCTG 3 cut(s) 250, 1079, 1958
PsuI RGATCY 2 cut(s) 364, 1221
PsyI GACNNNGTC 1 cut(s) 2065
PvuII CAGCTG 1 cut(s) 1031
SapI GCTCTTC 1 cut(s) 2541
SaqAI TTAA 5 cut(s) 156, 294, 566, 1725, 2373
Sau96I GGNCC 8 cut(s) 6, 52, 178, 346, 1430, 1518, 1883, 1921
ScaI AGTACT 1 cut(s) 841
SchI GAGTC 5 cut(s) 725, 1943, 2075, 2131, 2285
SduI GDGCHC 3 cut(s) 760, 934, 1983
SexAI ACCWGGT 1 cut(s) 1783
SfaNI GCATC 8 cut(s) 247, 753, 800, 806, 956, 1888, 1964, 2241
SfcI CTRYAG 1 cut(s) 2367
SfiI GGCCNNNNNGGCC 1 cut(s) 1215
SinI GGWCC 4 cut(s) 52, 178, 1883, 1921
SmlI CTYRAG 5 cut(s) 44, 565, 1752, 2073, 2255
SmoI CTYRAG 5 cut(s) 44, 565, 1752, 2073, 2255
SphI GCATGC 2 cut(s) 1510, 1657
Sse9I AATT 7 cut(s) 1675, 1726, 1828, 2028, 2181, 2212, 2242
SsiI CCGC 4 cut(s) 169, 494, 1550, 1609
SspI AATATT 2 cut(s) 1318, 2471
StyI CCWWGG 4 cut(s) 369, 1276, 2140, 2318
TaiI ACGT 5 cut(s) 646, 903, 1153, 2302, 2398
TaqI TCGA 4 cut(s) 33, 75, 2091, 2120
TaqII GACCGA 1 cut(s) 460
TasI AATT 7 cut(s) 1675, 1726, 1828, 2028, 2181, 2212, 2242
TatI WGTACW 3 cut(s) 839, 847, 2592
TauI GCSGC 1 cut(s) 1612
TfiI GAWTC 9 cut(s) 196, 428, 964, 1439, 1705, 1936, 1961, 1990, 2033
Tru1I TTAA 5 cut(s) 156, 294, 566, 1725, 2373
Tru9I TTAA 5 cut(s) 156, 294, 566, 1725, 2373
TscAI CASTG 6 cut(s) 211, 415, 934, 1219, 1258, 1423
TseFI GTSAC 2 cut(s) 1060, 1634
Tsp45I GTSAC 2 cut(s) 1060, 1634
TspGWI ACGGA 1 cut(s) 1586
TspRI CASTG 6 cut(s) 211, 415, 934, 1219, 1258, 1423
Tth111I GACNNNGTC 1 cut(s) 2065
Van91I CCANNNNNTGG 1 cut(s) 1790
Vha464I CTTAAG 1 cut(s) 565
VneI GTGCAC 2 cut(s) 756, 930
VpaK11BI GGWCC 4 cut(s) 52, 178, 1883, 1921
XapI RAATTY 2 cut(s) 1675, 1828
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 1510, 1657
XmaJI CCTAGG 1 cut(s) 1276
XmnI GAANNNNTTC 2 cut(s) 1483, 2164
ZrmI AGTACT 1 cut(s) 841
Zsp2I ATGCAT 1 cut(s) 2229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.