MD14G1244600.v1.1

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006861
Physical Location & Seq
Forward (+)
32270359 .. 32278214
7856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1244600.v1.1.491

Sequence Viewer

Length: 2697 bp
ATGGGGGCAAGTCGGAAGCTCCAGGGCGAGATCGACCGCGTTCTCAAGAAGGTCCAGGAAGGCGTCGATGTCTTCGATAGCATTTGGAACAAGGTTTACGATACGGACAATGCGAACCAGAAGGAGAAGTTTGAGGCCGACCTGAAGAAGGAGATTAAGAAGCTCCAGAGGTACAGGGACCAAATCAAGACTTGGATTCAGTCCAGTGAAATCAAGGATAAGAAGGTGAGTGCCTCTTATGAGCAGGCTCTGGTGGATGCTCGCAAACTCATTGAGCGCGAAATGGAAAGGTTTAAGATTTGTGAAAAGGAGACAAAAACAAAAGCATTTTCTAAAGAAGGATTGGGCCAACAACCTAAAACCGATCCGAGGGAGAAGGCCAAATCAGAGACAAGGGATTGGATAAACAACGTGGTTGGGGAGTTGGAATCGCAGATTGACAGCTTTGAAGCTGAGATTGAAGGGCTAACTTTCAAGAAAGGGAAGGGCAGGCCGCCCAGACTGACCCATCTAGAGACTTCAATTACTCGGCATAAGGCACATATAATGAAGTTAGAACTGATCTTGAGGCTCTTGGATAATGACGAGCTGAGTCCTGAGCAGGTCAATGATGTTAAAGACTTTTTGGAGGACTATGTGGAACGCAATCAGGAGGATTTTGATGAATTTAGTGAAGTTGATGAGCTTTACAACACCTTACCATTGGACAAGGTGGAGTCCCTTGAAGATCTGGCCATTATTCCTCCTGGTCTTATCAAGGGTGTACCGATGCTTTGCTTGAAGACTTCTTTGGCACCACTCGCATCTCCAATGCCTCCCGCTGTTACTTCTACTAATCAGCAGAGTGTTTCTGTACAAGAGCCAGTTGAAGATATAGTTTCCCAGGACAGTAATGCTGATAACATTCCTAGAACTCCACCTCCTAAAAGTGGTGCGCTTTCTTCTTCTCCTGCATCAACGCCAACTGGGAGTCATGCAACTCCTGTCTCTGCAAGTGTTTCAACTCAGAATCTGCCCAGTGTGCCAAGTGTTTTGGCCATTCCAGGTTTGAATGCTGTTCGGGGTGTCACAGAGAATGCAGGGGCTGCTAATTCTTCATCTCCTGTAAGCTTGTCTCCTTCTGTGAAGGAAGACGAATTAGCAAGCTTCCCTGGCCGTAGACCATCACCATCTCTTTCTGATGCTGGACTTGTAAGGGGCATCGGTCGAGGTGGCTTCTCTGCACCAATTCCATCTAGTATCCCTGTTAGCTCTAGCAATGTGGCTCCTAGTAATAGTGCCCTTGGTGCAGTTCCTTCAGTTTCTGATGTGACCAAGAGAAATATATTGGGAGCTGATGAGAGAATTGGGAGTAGTGGCGTGGTGCAGCCGCTTGTTTCCCCCATAACTAACAGACTGATCTTGCCTCAAGCTGCCAAGGCTAGTGACGTAAGTGCACCAGTTGATTCTAGCAATGCTAGTGAGGCTGGTATACCTGGCAGAGCATTTTCTCCGTCTATGGTCTCTGGCATGCAGTGGAGGCCTGGAAGTTCCTTCCAAAACCAAAATGAAGCAGGGATGTTTCGAGGAAGAACTGAAATTGCACCTGATCAGAGGGAGAAGTTTTTGCAGCGGCTCCAACAAGTGCAGCAACAAGGTCATAGTACCATTCTTGGCATGCCTCCTCTTACAAGTGGAAATCATAAGCAATTTTCTGCACAGCAAAACCCACTTTTGCAACAGTTTAATTCGCAAAACTCATCAGTGTCTTCTCAAGCTGGCCCTGGACTAGGAGTACAGCCCCCAGTTCTTGGTACTGTTCCACCTACCACCTTACAGCAGCAGCTAAATTCTATCCATCAACAATCTAATCAACAGGCATTGATGTCGAGTGGACCAAAAGAAGGTGATGTAAGTCATCCAAAAGTTGAGGACCAGCAACAACAACAGAATATACCTGATGATTCTACTGCAGACTCTACTCCTAATTCTGGACTCGTAAAGAATCTAATGAATGAAGATGACTTGAGCGCCTCATATGCAATTGATTCTCTGGCCGGAGTTTCTGGCTCTTCCACAGAGCCTGCTCAAGTACCACGAGATATTGATCTCTCTCATGGGCAACCTTTACAGTCCAATCAACCTTCTGCTAGCCTTGGTGTTATTGGCAGAAGAAGTGTTTCTGATCTCGGTGCAATTGGTGATAACCTTTCTGGATCAACTGCTAATTCTGGGGGAATGCATGATCAGTTGTATAACTTTCAGATGCTTGAGGCTGCTTACTACAAGCTTCCACAACCCAAAGACTCAGAACGTGCAAGGAGCTATAGTCCAAGGCACCCTGCAATAACACCTCTTAGCTACCCTCAAGCACAAGCACCTATTGTTAACAATCCTGCCTTCTGGGAACGGTTAGGTCTTGAACCATATGGCACTGATACACTGTTCTTTGCATTTTACTATCAGCAGAACACTTATCAGCAATATTTGGCTGCAAAAGAATTGAAGAAGCAATCTTGGAGATACCACAGGAAATATAACACTTGGTTTCAACGACATGAGGAGCCAAAAGTTGCCACGGATGAATATGAACAAGGGACGTATGTGTACTTTGACTTTCATATTGCAAATGATGATCTACAACATGGATGGTGCCAAAGGATCAAAACAGAGTTCACTTTTGAATATAACTATCTTGAAGATGAACTTATTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

899

Amino Acids

98.6

Weight (kDa)

5.32

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Not3 PF04065 4 - 235 3.1e-83 Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C PF04153 754 - 892 7.3e-34 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 3 cut(s) 793, 2316, 2631
AccB7I CCANNNNNTGG 1 cut(s) 1790
AccI GTMKAC 2 cut(s) 1159, 1471
AccII CGCG 2 cut(s) 39, 279
AciI CCGC 5 cut(s) 37, 494, 819, 1370, 1612
AclWI GGATC 3 cut(s) 359, 2203, 2648
AcoI YGGCCR 4 cut(s) 732, 1035, 1153, 2034
AcsI RAATTY 2 cut(s) 665, 1828
AcuI CTGAAG 2 cut(s) 164, 1281
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 8 cut(s) 173, 765, 855, 1645, 1776, 1795, 2073, 2588
AfiI CCNNNNNNNGG 7 cut(s) 148, 369, 929, 1769, 1790, 1883, 1970
AjnI CCWGG 9 cut(s) 21, 54, 745, 882, 1042, 1150, 1474, 1522, 1762
AjuI GAANNNNNNNTTGG 2 cut(s) 773, 805
AloI GAACNNNNNNTCC 2 cut(s) 904, 936
Alw21I GWGCWC 1 cut(s) 1438
Alw26I GTCTC 6 cut(s) 305, 383, 509, 991, 1119, 1507
Alw44I GTGCAC 1 cut(s) 1434
AlwI GGATC 3 cut(s) 359, 2203, 2648
AlwNI CAGNNNCTG 5 cut(s) 250, 1012, 1085, 1304, 2063
ApaLI GTGCAC 1 cut(s) 1434
ApeKI GCWGC 9 cut(s) 1085, 1366, 1412, 1609, 1627, 1819, 1822, 2255, 2471
ApoI RAATTY 2 cut(s) 665, 1828
Asp700I GAANNNNTTC 1 cut(s) 2158
AspLEI GCGC 3 cut(s) 279, 937, 2012
AspS9I GGNCC 6 cut(s) 52, 178, 346, 1760, 1874, 1912
AsuHPI GGTGA 4 cut(s) 238, 1158, 1898, 2192
AsuNHI GCTAGC 1 cut(s) 2129
AvaII GGWCC 4 cut(s) 52, 178, 1874, 1912
BaeGI GKGCMC 2 cut(s) 1282, 1438
BalI TGGCCA 2 cut(s) 734, 1037
BanI GGYRCC 3 cut(s) 793, 2316, 2631
BauI CACGAG 1 cut(s) 2076
BbsI GAAGAC 4 cut(s) 64, 788, 1137, 1740
Bbv12I GWGCWC 1 cut(s) 1438
BbvI GCAGC 9 cut(s) 1072, 1378, 1399, 1621, 1639, 1831, 1834, 2242, 2458
BccI CCATC 6 cut(s) 516, 1171, 1177, 1240, 1845, 2622
BceAI ACGGC 1 cut(s) 1140
BcgI CGANNNNNNTGC 2 cut(s) 1848, 1882
BciT130I CCWGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
BciVI GTATCC 1 cut(s) 1250
BclI TGATCA 2 cut(s) 1588, 2224
BcoDI GTCTC 6 cut(s) 305, 383, 509, 991, 1119, 1507
BfmI CTRYAG 2 cut(s) 1950, 2305
BfoI RGCGCY 1 cut(s) 2013
BfuAI ACCTGC 1 cut(s) 592
BfuI GTATCC 1 cut(s) 1250
BglII AGATCT 1 cut(s) 727
Bme1390I CCNGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
Bme18I GGWCC 4 cut(s) 52, 178, 1874, 1912
BmgT120I GGNCC 6 cut(s) 52, 178, 346, 1760, 1874, 1912
BmiI GGNNCC 7 cut(s) 179, 795, 1266, 1616, 2318, 2544, 2633
BmrFI CCNGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
BmrI ACTGGG 3 cut(s) 975, 1011, 1778
BmsI GCATC 7 cut(s) 247, 759, 812, 962, 1171, 1209, 2235
BmtI GCTAGC 1 cut(s) 2133
BmuI ACTGGG 3 cut(s) 975, 1011, 1778
BpiI GAAGAC 4 cut(s) 64, 788, 1137, 1740
BpmI CTGGAG 2 cut(s) 5, 149
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 8 cut(s) 29, 586, 1392, 1737, 2026, 2052, 2270, 2331
BsaBI GATNNNNATC 1 cut(s) 2085
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 1507
BsaXI ACNNNNNCTCC 4 cut(s) 904, 934, 2293, 2323
Bsc4I CCNNNNNNNGG 7 cut(s) 148, 369, 929, 1769, 1790, 1883, 1970
Bse1I ACTGG 6 cut(s) 204, 863, 970, 1017, 1439, 1784
Bse3DI GCAATG 2 cut(s) 1264, 1459
Bse8I GATNNNNATC 1 cut(s) 2085
BseBI CCWGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
BseGI GGATG 5 cut(s) 262, 1563, 1897, 2566, 2633
BseJI GATNNNNATC 1 cut(s) 2085
BseLI CCNNNNNNNGG 7 cut(s) 148, 369, 929, 1769, 1790, 1883, 1970
BseMI GCAATG 2 cut(s) 1264, 1459
BseMII CTCAG 5 cut(s) 444, 581, 588, 1019, 2301
BseNI ACTGG 6 cut(s) 204, 863, 970, 1017, 1439, 1784
BseRI GAGGAG 2 cut(s) 1653, 2555
BseSI GKGCMC 2 cut(s) 1282, 1438
BseXI GCAGC 9 cut(s) 1072, 1378, 1399, 1621, 1639, 1831, 1834, 2242, 2458
BsgI GTGCAG 5 cut(s) 1206, 1308, 1385, 1646, 1680
Bsh1236I CGCG 2 cut(s) 39, 279
Bsh1285I CGRYCG 2 cut(s) 37, 1207
BshNI GGYRCC 3 cut(s) 793, 2316, 2631
BsiEI CGRYCG 2 cut(s) 37, 1207
BsiHKAI GWGCWC 1 cut(s) 1438
BsiSI CCGG 1 cut(s) 2037
BslFI GGGAC 3 cut(s) 191, 703, 2590
BslI CCNNNNNNNGG 7 cut(s) 148, 369, 929, 1769, 1790, 1883, 1970
BsmAI GTCTC 6 cut(s) 305, 383, 509, 991, 1119, 1507
BsmFI GGGAC 3 cut(s) 191, 703, 2590
BsmI GAATGC 3 cut(s) 1057, 1081, 2223
Bso31I GGTCTC 1 cut(s) 1507
Bsp1286I GDGCHC 2 cut(s) 1282, 1438
Bsp1407I TGTACA 1 cut(s) 853
BspACI CCGC 5 cut(s) 37, 494, 819, 1370, 1612
BspCNI CTCAG 5 cut(s) 445, 582, 589, 1018, 2300
BspFNI CGCG 2 cut(s) 39, 279
BspLI GGNNCC 7 cut(s) 179, 795, 1266, 1616, 2318, 2544, 2633
BspMAI CTGCAG 1 cut(s) 1954
BspMI ACCTGC 1 cut(s) 592
BspOI GCTAGC 1 cut(s) 2133
BspPI GGATC 3 cut(s) 359, 2203, 2648
BspQI GCTCTTC 1 cut(s) 2056
BspT107I GGYRCC 3 cut(s) 793, 2316, 2631
BspTNI GGTCTC 1 cut(s) 1507
BsrDI GCAATG 2 cut(s) 1264, 1459
BsrGI TGTACA 1 cut(s) 853
BsrI ACTGG 6 cut(s) 204, 863, 970, 1017, 1439, 1784
BssNAI GTATAC 1 cut(s) 1472
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 2076
BssT1I CCWWGG 4 cut(s) 1282, 1416, 2134, 2312
Bst1107I GTATAC 1 cut(s) 1472
Bst2BI CACGAG 1 cut(s) 2076
Bst2UI CCWGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
Bst4CI ACNGT 6 cut(s) 890, 1722, 1798, 2112, 2391, 2424
Bst6I CTCTTC 1 cut(s) 2056
BstACI GRCGYC 1 cut(s) 63
BstAPI GCANNNNNTGC 1 cut(s) 1085
BstAUI TGTACA 1 cut(s) 853
BstC8I GCNNGC 9 cut(s) 246, 262, 491, 1144, 1511, 1658, 1759, 2064, 2131
BstDEI CTNAG 6 cut(s) 453, 590, 597, 1005, 2287, 2336
BstDSI CCRYGG 1 cut(s) 2556
BstENI CCTNNNNNAGG 2 cut(s) 146, 1767
BstF5I GGATG 5 cut(s) 262, 1563, 1897, 2566, 2633
BstFNI CGCG 2 cut(s) 39, 279
BstH2I RGCGCY 1 cut(s) 2013
BstHHI GCGC 3 cut(s) 279, 937, 2012
BstMAI GTCTC 6 cut(s) 305, 383, 509, 991, 1119, 1507
BstMCI CGRYCG 2 cut(s) 37, 1207
BstMWI GCNNNNNNNGC 9 cut(s) 800, 1021, 1085, 1152, 1286, 1418, 1463, 1519, 2018
BstNI CCWGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
BstNSI RCATGY 2 cut(s) 1513, 1660
BstSCI CCNGG 9 cut(s) 21, 54, 745, 882, 1042, 1150, 1474, 1522, 1762
BstSFI CTRYAG 2 cut(s) 1950, 2305
BstSLI GKGCMC 2 cut(s) 1282, 1438
BstUI CGCG 2 cut(s) 39, 279
BstV1I GCAGC 9 cut(s) 1072, 1378, 1399, 1621, 1639, 1831, 1834, 2242, 2458
BstV2I GAAGAC 4 cut(s) 64, 788, 1137, 1740
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BstZ17I GTATAC 1 cut(s) 1472
BsuI GTATCC 1 cut(s) 1250
BtgI CCRYGG 1 cut(s) 2556
BtsCI GGATG 5 cut(s) 262, 1563, 1897, 2566, 2633
BtsI GCAGTG 1 cut(s) 1520
BtsIMutI CAGTG 6 cut(s) 211, 1024, 1520, 1749, 2412, 2420
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 9 cut(s) 246, 262, 491, 1144, 1511, 1658, 1759, 2064, 2131
CaiI CAGNNNCTG 5 cut(s) 250, 1012, 1085, 1304, 2063
CfoI GCGC 3 cut(s) 279, 937, 2012
Cfr13I GGNCC 6 cut(s) 52, 178, 346, 1760, 1874, 1912
CseI GACGC 1 cut(s) 52
Csp6I GTAC 8 cut(s) 172, 764, 854, 1644, 1775, 1794, 2072, 2587
CviAII CATG 7 cut(s) 974, 1510, 1657, 2096, 2222, 2537, 2624
CviQI GTAC 8 cut(s) 172, 764, 854, 1644, 1775, 1794, 2072, 2587
DdeI CTNAG 6 cut(s) 453, 590, 597, 1005, 2287, 2336
EaeI YGGCCR 4 cut(s) 732, 1035, 1153, 2034
Eam1104I CTCTTC 1 cut(s) 2056
EarI CTCTTC 1 cut(s) 2056
Eco130I CCWWGG 4 cut(s) 1282, 1416, 2134, 2312
Eco147I AGGCCT 1 cut(s) 1522
Eco31I GGTCTC 1 cut(s) 1507
Eco47I GGWCC 4 cut(s) 52, 178, 1874, 1912
Eco57I CTGAAG 2 cut(s) 164, 1281
EcoNI CCTNNNNNAGG 2 cut(s) 146, 1767
EcoRII CCWGG 9 cut(s) 21, 54, 745, 882, 1042, 1150, 1474, 1522, 1762
EcoT14I CCWWGG 4 cut(s) 1282, 1416, 2134, 2312
EcoT22I ATGCAT 1 cut(s) 2223
ErhI CCWWGG 4 cut(s) 1282, 1416, 2134, 2312
FaeI CATG 7 cut(s) 977, 1513, 1660, 2099, 2225, 2540, 2627
FalI AAGNNNNNCTT 1 cut(s) 2670
FaqI GGGAC 3 cut(s) 191, 703, 2590
FatI CATG 7 cut(s) 973, 1509, 1656, 2095, 2221, 2536, 2623
FauI CCCGC 1 cut(s) 826
FauNDI CATATG 2 cut(s) 2017, 2407
FbaI TGATCA 2 cut(s) 1588, 2224
FblI GTMKAC 2 cut(s) 1159, 1471
FokI GGATG 5 cut(s) 269, 1570, 1884, 2573, 2640
GlaI GCGC 3 cut(s) 278, 936, 2011
GsuI CTGGAG 2 cut(s) 5, 149
HaeII RGCGCY 1 cut(s) 2013
HapII CCGG 1 cut(s) 2037
HgaI GACGC 1 cut(s) 52
HhaI GCGC 3 cut(s) 279, 937, 2012
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 7 cut(s) 977, 1513, 1660, 2099, 2225, 2540, 2627
Hin6I GCGC 3 cut(s) 277, 935, 2010
HinP1I GCGC 3 cut(s) 277, 935, 2010
HincII GTYRAC 1 cut(s) 2368
HindII GTYRAC 1 cut(s) 2368
HindIII AAGCTT 3 cut(s) 1108, 1144, 2267
HpaI GTTAAC 1 cut(s) 2368
HpaII CCGG 1 cut(s) 2037
HphI GGTGA 4 cut(s) 238, 1158, 1898, 2192
Hpy166II GTNNAC 9 cut(s) 97, 764, 1160, 1436, 1472, 1874, 2368, 2587, 2655
Hpy8I GTNNAC 9 cut(s) 97, 764, 1160, 1436, 1472, 1874, 2368, 2587, 2655
Hpy99I CGWCG 1 cut(s) 68
HpyCH4III ACNGT 6 cut(s) 890, 1722, 1798, 2112, 2391, 2424
HpyCH4IV ACGT 4 cut(s) 411, 1428, 2293, 2579
HpyF10VI GCNNNNNNNGC 9 cut(s) 800, 1021, 1085, 1152, 1286, 1418, 1463, 1519, 2018
HpyF3I CTNAG 6 cut(s) 453, 590, 597, 1005, 2287, 2336
HpySE526I ACGT 4 cut(s) 411, 1428, 2293, 2579
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 7 cut(s) 977, 1513, 1660, 2099, 2225, 2540, 2627
HspAI GCGC 3 cut(s) 277, 935, 2010
Ksp22I TGATCA 2 cut(s) 1588, 2224
KspAI GTTAAC 1 cut(s) 2368
LguI GCTCTTC 1 cut(s) 2056
LmnI GCTCC 7 cut(s) 24, 168, 1270, 1331, 1620, 2301, 2542
Lsp1109I GCAGC 9 cut(s) 1072, 1378, 1399, 1621, 1639, 1831, 1834, 2242, 2458
LweI GCATC 7 cut(s) 247, 759, 812, 962, 1171, 1209, 2235
MaeII ACGT 4 cut(s) 411, 1428, 2293, 2579
MaeIII GTNAC 4 cut(s) 823, 1066, 1309, 1424
MfeI CAATTG 2 cut(s) 2022, 2175
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 2 cut(s) 1282, 1438
MlsI TGGCCA 2 cut(s) 734, 1037
MluNI TGGCCA 2 cut(s) 734, 1037
MlyI GAGTC 6 cut(s) 601, 725, 979, 1949, 1968, 2279
MmeI TCCRAC 2 cut(s) 405, 1642
Mox20I TGGCCA 2 cut(s) 734, 1037
Mph1103I ATGCAT 1 cut(s) 2223
MroXI GAANNNNTTC 1 cut(s) 2158
MscI TGGCCA 2 cut(s) 734, 1037
MseI TTAA 5 cut(s) 156, 294, 615, 1725, 2367
Msp20I TGGCCA 2 cut(s) 734, 1037
MspA1I CMGCKG 2 cut(s) 821, 1612
MspI CCGG 1 cut(s) 2037
MspR9I CCNGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
MunI CAATTG 2 cut(s) 2022, 2175
Mva1269I GAATGC 3 cut(s) 1057, 1081, 2223
MvaI CCWGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
MvnI CGCG 2 cut(s) 39, 279
MwoI GCNNNNNNNGC 9 cut(s) 800, 1021, 1085, 1152, 1286, 1418, 1463, 1519, 2018
NdeI CATATG 2 cut(s) 2017, 2407
NheI GCTAGC 1 cut(s) 2129
NlaIII CATG 7 cut(s) 977, 1513, 1660, 2099, 2225, 2540, 2627
NlaIV GGNNCC 7 cut(s) 179, 795, 1266, 1616, 2318, 2544, 2633
NmeAIII GCCGAG 1 cut(s) 508
NmuCI GTSAC 3 cut(s) 1066, 1309, 1424
NsiI ATGCAT 1 cut(s) 2223
NspI RCATGY 2 cut(s) 1513, 1660
PaeI GCATGC 2 cut(s) 1513, 1660
PceI AGGCCT 1 cut(s) 1522
PciSI GCTCTTC 1 cut(s) 2056
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 110
PctI GAATGC 3 cut(s) 1057, 1081, 2223
PdmI GAANNNNTTC 1 cut(s) 2158
PfeI GAWTC 7 cut(s) 196, 428, 1009, 1445, 1943, 1984, 2027
PflMI CCANNNNNTGG 1 cut(s) 1790
PfoI TCCNGGA 1 cut(s) 54
PleI GAGTC 6 cut(s) 600, 724, 978, 1949, 1968, 2279
PpsI GAGTC 6 cut(s) 600, 724, 978, 1949, 1968, 2279
Psp6I CCWGG 9 cut(s) 21, 54, 745, 882, 1042, 1150, 1474, 1522, 1762
PspGI CCWGG 9 cut(s) 21, 54, 745, 882, 1042, 1150, 1474, 1522, 1762
PspN4I GGNNCC 7 cut(s) 179, 795, 1266, 1616, 2318, 2544, 2633
PspPI GGNCC 6 cut(s) 52, 178, 346, 1760, 1874, 1912
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstI CTGCAG 1 cut(s) 1954
PstNI CAGNNNCTG 5 cut(s) 250, 1012, 1085, 1304, 2063
PsuI RGATCY 1 cut(s) 727
RsaI GTAC 8 cut(s) 173, 765, 855, 1645, 1776, 1795, 2073, 2588
RsaNI GTAC 8 cut(s) 172, 764, 854, 1644, 1775, 1794, 2072, 2587
SapI GCTCTTC 1 cut(s) 2056
SaqAI TTAA 5 cut(s) 156, 294, 615, 1725, 2367
Sau96I GGNCC 6 cut(s) 52, 178, 346, 1760, 1874, 1912
SchI GAGTC 6 cut(s) 601, 725, 979, 1949, 1968, 2279
ScrFI CCNGG 9 cut(s) 23, 56, 747, 884, 1044, 1152, 1476, 1524, 1764
SduI GDGCHC 2 cut(s) 1282, 1438
SfaNI GCATC 7 cut(s) 247, 759, 812, 962, 1171, 1209, 2235
SfcI CTRYAG 2 cut(s) 1950, 2305
SinI GGWCC 4 cut(s) 52, 178, 1874, 1912
SmlI CTYRAG 8 cut(s) 44, 565, 1407, 1752, 2005, 2067, 2249, 2346
SmoI CTYRAG 8 cut(s) 44, 565, 1407, 1752, 2005, 2067, 2249, 2346
SphI GCATGC 2 cut(s) 1513, 1660
SseBI AGGCCT 1 cut(s) 1522
SsiI CCGC 5 cut(s) 37, 494, 819, 1370, 1612
SspI AATATT 1 cut(s) 2465
StuI AGGCCT 1 cut(s) 1522
StyD4I CCNGG 9 cut(s) 21, 54, 745, 882, 1042, 1150, 1474, 1522, 1762
StyI CCWWGG 4 cut(s) 1282, 1416, 2134, 2312
TaaI ACNGT 6 cut(s) 890, 1722, 1798, 2112, 2391, 2424
TaiI ACGT 4 cut(s) 414, 1431, 2296, 2582
TaqI TCGA 6 cut(s) 33, 66, 75, 1207, 1564, 1868
TaqII GACCGA 1 cut(s) 1193
TatI WGTACW 3 cut(s) 853, 1774, 2586
TauI GCSGC 3 cut(s) 496, 1372, 1615
TfiI GAWTC 7 cut(s) 196, 428, 1009, 1445, 1943, 1984, 2027
Tru1I TTAA 5 cut(s) 156, 294, 615, 1725, 2367
Tru9I TTAA 5 cut(s) 156, 294, 615, 1725, 2367
TscAI CASTG 6 cut(s) 211, 1024, 1520, 1749, 2419, 2427
TseFI GTSAC 3 cut(s) 1066, 1309, 1424
TseI GCWGC 9 cut(s) 1085, 1366, 1412, 1609, 1627, 1819, 1822, 2255, 2471
Tsp45I GTSAC 3 cut(s) 1066, 1309, 1424
TspGWI ACGGA 3 cut(s) 119, 1482, 2573
TspRI CASTG 6 cut(s) 211, 1024, 1520, 1749, 2419, 2427
Van91I CCANNNNNTGG 1 cut(s) 1790
VneI GTGCAC 1 cut(s) 1434
VpaK11BI GGWCC 4 cut(s) 52, 178, 1874, 1912
XagI CCTNNNNNAGG 2 cut(s) 146, 1767
XapI RAATTY 2 cut(s) 665, 1828
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 1513, 1660
XmiI GTMKAC 2 cut(s) 1159, 1471
XmnI GAANNNNTTC 1 cut(s) 2158
Zsp2I ATGCAT 1 cut(s) 2223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.