AT5G18460

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013071
Physical Location & Seq
Forward (+)
6122725 .. 6126742
4018 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18460.1

Sequence Viewer

Length: 1293 bp
ATGGAGGTGAATGTTCTTGCATCCAAGAGAAGTCTTCTCCTTTTTCTTCTTATTGTAAGTCTCTCTTTGAGCCAGAAGGTCAGACATATCTCTGCCTTTAACGACACCAAACCATACAGACAAGTCAGTAGCTTGAGACTTGCGAGGATCCAAAAGCACTTGAACAAGATCAACAAGTCTCCTGTCTTCACCATTCAGAGTCCAGATGGTGATGTAATAGATTGTGTACCCAAAAGAAAGCAGCCAGCTTTGGATCATCCACTCTTAAAGCATCACAAGATTCAGAAAGCACCGAAGAAAATGCCGAAGATGAAGGGAAAAGATGATGATGTTAAAGAAGCAGAGAATGTATTGGAAGGTGCATGGCAAATGTGGCACGTGAACGGCACGAGGTGCCCTAAGGGGACCGTTCCCATACGACGCAACACAATGAACGATGTGCTCAGAGCCAAGTCTTTGTTTGACTTTGGCAAAAAGCGACGGAGTATTTACCTTGATCAACGGACAGAAAAGCCTGATGCCCTTGGTACCAATGGACATGAGCATGCGATCGCTTATACAGAATCATCGTCGGAGATATATGGAGCAAAGGCGACTATAAACGTTTGGGATCCAAAGATAGAAGAAGTGAATGAGTTTAGTCTCTCTCAGATTTGGATTCTCTCTGGATCTTTCGTAGGTCCTGATCTCAATAGTATTGAAGCTGGCTGGCAGGTCAGTCCGGAGCTGTATGGTGACAACAGACCAAGATTGTTCACTTATTGGACGAGTGATTCATATCAAGCAACAGGATGTTACAATCTTCTATGTTCGGGATTTATCCAAACAAATAACAAGATTGCCATTGGAGCAGCCATATCTCCTCTTTCTACGTTCAAAGGAAACCAGTTTGATATCACAATACTCATTTGGAAGGATCCGAAAATGGGGAATTGGTGGATGGGTTTAGGGGATAGCACACTAGTCGGGTATTGGCCAGCCGAGCTATTCACGCACTTGGCCGACCACGCGACTACCGTGGAATGGGGCGGTGAAGTCGTCAACACACGTGCTTCAGGCAGGCACACGACGACCCAAATGGGCTCTGGTCATTTTCCAGACGAAGGGTTTGGGAAAGCAAGTTATTTTCGGAATTTGGAAGTTGTGGATTCGGACAACAGTCTCGTACCGGTGCATGATGTCAAGATACTAGCCGAGAACACCGAATGTTACGACATTAAGAGTTCGTATAGTAATGAATGGGGAACTTATTTTTACTATGGTGGGCCGGGGTTTAATCCTAGGTGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

430

Amino Acids

48.44

Weight (kDa)

8.54

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 64 - 186 3e-44 Neprosin activation peptide
Neprosin PF03080 211 - 423 4.8e-89 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 703
Acc65I GGTACC 1 cut(s) 527
AccB1I GGYRCC 2 cut(s) 393, 527
AccII CGCG 1 cut(s) 1010
AccIII TCCGGA 1 cut(s) 721
AciI CCGC 1 cut(s) 1029
AclI AACGTT 1 cut(s) 603
AclWI GGATC 8 cut(s) 142, 155, 261, 605, 618, 676, 911, 924
AcoI YGGCCR 2 cut(s) 974, 999
AcsI RAATTY 1 cut(s) 1132
AcuI CTGAAG 1 cut(s) 1038
AcvI CACGTG 2 cut(s) 379, 1049
AdeI CACNNNGTG 1 cut(s) 393
AfaI GTAC 3 cut(s) 228, 529, 1167
AfiI CCNNNNNNNGG 3 cut(s) 926, 1023, 1103
AflIII ACRYGT 1 cut(s) 1046
AgeI ACCGGT 1 cut(s) 1168
AgsI TTSAA 3 cut(s) 163, 701, 877
AhlI ACTAGT 1 cut(s) 961
AluBI AGCT 5 cut(s) 132, 248, 704, 727, 985
AluI AGCT 5 cut(s) 132, 248, 704, 727, 985
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 5 cut(s) 65, 130, 183, 647, 1166
AlwI GGATC 8 cut(s) 142, 155, 261, 605, 618, 676, 911, 924
Aor13HI TCCGGA 1 cut(s) 721
AoxI GGCC 3 cut(s) 974, 999, 1265
ApeKI GCWGC 2 cut(s) 241, 851
ApoI RAATTY 1 cut(s) 1132
ArsI GACNNNNNNTTYG 2 cut(s) 1091, 1123
AsiGI ACCGGT 1 cut(s) 1168
AsiSI GCGATCGC 1 cut(s) 552
Asp718I GGTACC 1 cut(s) 527
AspA2I CCTAGG 1 cut(s) 1280
AspS9I GGNCC 3 cut(s) 405, 680, 1265
AsuC2I CCSGG 1 cut(s) 1269
AsuHPI GGTGA 5 cut(s) 19, 181, 221, 746, 1043
AvaII GGWCC 2 cut(s) 405, 680
AvrII CCTAGG 1 cut(s) 1280
AxyI CCTNAGG 1 cut(s) 399
BaeGI GKGCMC 1 cut(s) 398
BalI TGGCCA 1 cut(s) 976
BamHI GGATCC 3 cut(s) 147, 610, 916
BanI GGYRCC 2 cut(s) 393, 527
BanII GRGCYC 1 cut(s) 1085
BauI CACGAG 1 cut(s) 388
BbrPI CACGTG 2 cut(s) 379, 1049
BbsI GAAGAC 2 cut(s) 26, 178
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 2 cut(s) 253, 863
BccI CCATC 2 cut(s) 200, 934
BceAI ACGGC 1 cut(s) 400
BcgI CGANNNNNNTGC 4 cut(s) 283, 317, 946, 980
BclI TGATCA 1 cut(s) 496
BcnI CCSGG 1 cut(s) 1269
BcoDI GTCTC 5 cut(s) 65, 130, 183, 647, 1166
BcuI ACTAGT 1 cut(s) 961
BfaI CTAG 3 cut(s) 962, 1190, 1281
BfuAI ACCTGC 1 cut(s) 703
BisI GCNGC 2 cut(s) 242, 852
BlnI CCTAGG 1 cut(s) 1280
BlsI GCNGC 2 cut(s) 243, 853
Bme1390I CCNGG 1 cut(s) 1269
Bme18I GGWCC 2 cut(s) 405, 680
BmgT120I GGNCC 3 cut(s) 405, 680, 1265
BmiI GGNNCC 6 cut(s) 149, 395, 406, 529, 612, 918
BmrFI CCNGG 1 cut(s) 1269
BmsI GCATC 3 cut(s) 29, 280, 508
BoxI GACNNNNGTC 1 cut(s) 1158
BpiI GAAGAC 2 cut(s) 26, 178
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 1269
BsaAI YACGTR 2 cut(s) 379, 1049
BsaBI GATNNNNATC 1 cut(s) 777
BsaJI CCNNGG 4 cut(s) 523, 1017, 1268, 1280
BsaWI WCCGGW 2 cut(s) 721, 1168
Bsc4I CCNNNNNNNGG 3 cut(s) 926, 1023, 1103
Bse118I RCCGGY 1 cut(s) 1168
Bse1I ACTGG 1 cut(s) 886
Bse21I CCTNAGG 1 cut(s) 399
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 721
BseDI CCNNGG 4 cut(s) 523, 1017, 1268, 1280
BseGI GGATG 4 cut(s) 20, 256, 797, 945
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 3 cut(s) 926, 1023, 1103
BseMII CTCAG 2 cut(s) 457, 662
BseNI ACTGG 1 cut(s) 886
BseRI GAGGAG 1 cut(s) 852
BseSI GKGCMC 1 cut(s) 398
BseXI GCAGC 2 cut(s) 253, 863
Bsh1236I CGCG 1 cut(s) 1010
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 3 cut(s) 976, 1001, 1267
BshNI GGYRCC 2 cut(s) 393, 527
BshTI ACCGGT 1 cut(s) 1168
BsiEI CGRYCG 1 cut(s) 552
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 3 cut(s) 722, 1169, 1268
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 3 cut(s) 926, 1023, 1103
BsmAI GTCTC 5 cut(s) 65, 130, 183, 647, 1166
BsmFI GGGAC 1 cut(s) 418
BsnI GGCC 3 cut(s) 976, 1001, 1267
Bsp1286I GDGCHC 3 cut(s) 398, 444, 1085
Bsp13I TCCGGA 1 cut(s) 721
Bsp143I GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
BspACI CCGC 1 cut(s) 1029
BspANI GGCC 3 cut(s) 976, 1001, 1267
BspCNI CTCAG 2 cut(s) 456, 661
BspEI TCCGGA 1 cut(s) 721
BspFNI CGCG 1 cut(s) 1010
BspLI GGNNCC 6 cut(s) 149, 395, 406, 529, 612, 918
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 8 cut(s) 142, 155, 261, 605, 618, 676, 911, 924
BspT107I GGYRCC 2 cut(s) 393, 527
BsrFI RCCGGY 1 cut(s) 1168
BsrI ACTGG 1 cut(s) 886
BssAI RCCGGY 1 cut(s) 1168
BssECI CCNNGG 4 cut(s) 523, 1017, 1268, 1280
BssMI GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
BssSI CACGAG 1 cut(s) 388
BssT1I CCWWGG 2 cut(s) 523, 1280
Bst2BI CACGAG 1 cut(s) 388
Bst4CI ACNGT 3 cut(s) 409, 1018, 1160
BstAPI GCANNNNNTGC 1 cut(s) 393
BstBAI YACGTR 2 cut(s) 379, 1049
BstC8I GCNNGC 6 cut(s) 246, 546, 706, 710, 978, 1061
BstDEI CTNAG 3 cut(s) 399, 443, 648
BstDSI CCRYGG 1 cut(s) 1017
BstF5I GGATG 4 cut(s) 20, 256, 797, 945
BstFNI CGCG 1 cut(s) 1010
BstKTI GATC 9 cut(s) 150, 171, 256, 499, 552, 613, 671, 688, 919
BstMAI GTCTC 5 cut(s) 65, 130, 183, 647, 1166
BstMBI GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 6 cut(s) 373, 393, 848, 982, 991, 1007
BstNSI RCATGY 1 cut(s) 548
BstPAI GACNNNNGTC 1 cut(s) 1158
BstSCI CCNGG 1 cut(s) 1267
BstSLI GKGCMC 1 cut(s) 398
BstUI CGCG 1 cut(s) 1010
BstV1I GCAGC 2 cut(s) 253, 863
BstV2I GAAGAC 2 cut(s) 26, 178
BstX2I RGATCY 4 cut(s) 147, 610, 668, 916
BstYI RGATCY 4 cut(s) 147, 610, 668, 916
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 3 cut(s) 976, 1001, 1267
BtgI CCRYGG 1 cut(s) 1017
BtsCI GGATG 4 cut(s) 20, 256, 797, 945
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 6 cut(s) 246, 546, 706, 710, 978, 1061
Cfr10I RCCGGY 1 cut(s) 1168
Cfr13I GGNCC 3 cut(s) 405, 680, 1265
CseI GACGC 1 cut(s) 429
Csp6I GTAC 3 cut(s) 227, 528, 1166
CspAI ACCGGT 1 cut(s) 1168
CviAII CATG 4 cut(s) 363, 539, 545, 1175
CviQI GTAC 3 cut(s) 227, 528, 1166
DdeI CTNAG 3 cut(s) 399, 443, 648
DpnI GATC 9 cut(s) 149, 170, 255, 498, 551, 612, 670, 687, 918
DpnII GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
DraIII CACNNNGTG 1 cut(s) 393
EaeI YGGCCR 2 cut(s) 974, 999
Eco130I CCWWGG 2 cut(s) 523, 1280
Eco24I GRGCYC 1 cut(s) 1085
Eco32I GATATC 1 cut(s) 895
Eco47I GGWCC 2 cut(s) 405, 680
Eco57I CTGAAG 1 cut(s) 1038
Eco72I CACGTG 2 cut(s) 379, 1049
Eco81I CCTNAGG 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 680
EcoRV GATATC 1 cut(s) 895
EcoT14I CCWWGG 2 cut(s) 523, 1280
EcoT38I GRGCYC 1 cut(s) 1085
ErhI CCWWGG 2 cut(s) 523, 1280
FaeI CATG 4 cut(s) 366, 542, 548, 1178
FalI AAGNNNNNCTT 2 cut(s) 49, 81
FaqI GGGAC 1 cut(s) 418
FatI CATG 4 cut(s) 362, 538, 544, 1174
FbaI TGATCA 1 cut(s) 496
Fnu4HI GCNGC 2 cut(s) 242, 852
FokI GGATG 4 cut(s) 7, 243, 804, 952
FriOI GRGCYC 1 cut(s) 1085
Fsp4HI GCNGC 2 cut(s) 242, 852
FspBI CTAG 3 cut(s) 962, 1190, 1281
GluI GCNGC 2 cut(s) 242, 852
HaeIII GGCC 3 cut(s) 976, 1001, 1267
HapII CCGG 3 cut(s) 722, 1169, 1268
HgaI GACGC 1 cut(s) 429
Hin1II CATG 4 cut(s) 366, 542, 548, 1178
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HinfI GANTC 6 cut(s) 199, 280, 563, 658, 773, 1148
HpaII CCGG 3 cut(s) 722, 1169, 1268
HphI GGTGA 5 cut(s) 19, 181, 221, 746, 1043
Hpy166II GTNNAC 4 cut(s) 227, 382, 756, 1042
Hpy188I TCNGA 9 cut(s) 83, 198, 285, 446, 574, 651, 921, 1131, 1153
Hpy188III TCNNGA 7 cut(s) 203, 666, 683, 722, 813, 1097, 1183
Hpy8I GTNNAC 4 cut(s) 227, 382, 756, 1042
Hpy99I CGWCG 4 cut(s) 423, 483, 574, 1072
HpyAV CCTTC 5 cut(s) 70, 307, 350, 907, 1097
HpyCH4III ACNGT 3 cut(s) 409, 1018, 1160
HpyCH4IV ACGT 4 cut(s) 378, 603, 872, 1048
HpyCH4V TGCA 3 cut(s) 20, 362, 1174
HpyF10VI GCNNNNNNNGC 6 cut(s) 373, 393, 848, 982, 991, 1007
HpyF3I CTNAG 3 cut(s) 399, 443, 648
HpySE526I ACGT 4 cut(s) 378, 603, 872, 1048
Hsp92II CATG 4 cut(s) 366, 542, 548, 1178
Kpn2I TCCGGA 1 cut(s) 721
KpnI GGTACC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
LmnI GCTCC 3 cut(s) 584, 724, 848
Lsp1109I GCAGC 2 cut(s) 253, 863
LweI GCATC 3 cut(s) 29, 280, 508
MaeI CTAG 3 cut(s) 962, 1190, 1281
MaeII ACGT 4 cut(s) 378, 603, 872, 1048
MaeIII GTNAC 3 cut(s) 734, 794, 1208
MalI GATC 9 cut(s) 149, 170, 255, 498, 551, 612, 670, 687, 918
MboI GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
MboII GAAGA 7 cut(s) 26, 38, 178, 307, 319, 635, 794
MflI RGATCY 4 cut(s) 147, 610, 668, 916
MhlI GDGCHC 3 cut(s) 398, 444, 1085
MlsI TGGCCA 1 cut(s) 976
MluCI AATT 3 cut(s) 931, 1132, 1288
MluNI TGGCCA 1 cut(s) 976
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 3 cut(s) 138, 384, 873
Mox20I TGGCCA 1 cut(s) 976
MroI TCCGGA 1 cut(s) 721
MscI TGGCCA 1 cut(s) 976
MseI TTAA 5 cut(s) 99, 266, 333, 1218, 1275
MslI CAYNNNNRTG 1 cut(s) 543
Msp20I TGGCCA 1 cut(s) 976
MspI CCGG 3 cut(s) 722, 1169, 1268
MspR9I CCNGG 1 cut(s) 1269
MvnI CGCG 1 cut(s) 1010
MwoI GCNNNNNNNGC 6 cut(s) 373, 393, 848, 982, 991, 1007
NciI CCSGG 1 cut(s) 1269
NdeII GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
NlaIII CATG 4 cut(s) 366, 542, 548, 1178
NlaIV GGNNCC 6 cut(s) 149, 395, 406, 529, 612, 918
NmeAIII GCCGAG 2 cut(s) 1006, 1219
NmuCI GTSAC 1 cut(s) 734
NspI RCATGY 1 cut(s) 548
PaeI GCATGC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 1 cut(s) 1014
PfeI GAWTC 5 cut(s) 280, 563, 658, 773, 1148
PinAI ACCGGT 1 cut(s) 1168
PkrI GCNGC 2 cut(s) 243, 853
Ple19I CGATCG 1 cut(s) 552
PleI GAGTC 1 cut(s) 207
PmaCI CACGTG 2 cut(s) 379, 1049
PmlI CACGTG 2 cut(s) 379, 1049
PpsI GAGTC 1 cut(s) 207
Ppu21I YACGTR 2 cut(s) 379, 1049
PpuMI RGGWCCY 1 cut(s) 680
PshAI GACNNNNGTC 1 cut(s) 1158
Psp1406I AACGTT 1 cut(s) 603
Psp5II RGGWCCY 1 cut(s) 680
PspCI CACGTG 2 cut(s) 379, 1049
PspN4I GGNNCC 6 cut(s) 149, 395, 406, 529, 612, 918
PspPI GGNCC 3 cut(s) 405, 680, 1265
PspPPI RGGWCCY 1 cut(s) 680
PsuI RGATCY 4 cut(s) 147, 610, 668, 916
PvuI CGATCG 1 cut(s) 552
RgaI GCGATCGC 1 cut(s) 552
RsaI GTAC 3 cut(s) 228, 529, 1167
RsaNI GTAC 3 cut(s) 227, 528, 1166
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 5 cut(s) 99, 266, 333, 1218, 1275
SatI GCNGC 2 cut(s) 242, 852
Sau3AI GATC 9 cut(s) 147, 168, 253, 496, 549, 610, 668, 685, 916
Sau96I GGNCC 3 cut(s) 405, 680, 1265
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 1269
SduI GDGCHC 3 cut(s) 398, 444, 1085
SfaAI GCGATCGC 1 cut(s) 552
SfaNI GCATC 3 cut(s) 29, 280, 508
SgfI GCGATCGC 1 cut(s) 552
SinI GGWCC 2 cut(s) 405, 680
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
SpeI ACTAGT 1 cut(s) 961
SphI GCATGC 1 cut(s) 548
Sse9I AATT 3 cut(s) 931, 1132, 1288
SsiI CCGC 1 cut(s) 1029
SspMI CTAG 3 cut(s) 962, 1190, 1281
StyD4I CCNGG 1 cut(s) 1267
StyI CCWWGG 2 cut(s) 523, 1280
TaaI ACNGT 3 cut(s) 409, 1018, 1160
TaiI ACGT 4 cut(s) 381, 606, 875, 1051
TasI AATT 3 cut(s) 931, 1132, 1288
TfiI GAWTC 5 cut(s) 280, 563, 658, 773, 1148
Tru1I TTAA 5 cut(s) 99, 266, 333, 1218, 1275
Tru9I TTAA 5 cut(s) 99, 266, 333, 1218, 1275
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 2 cut(s) 241, 851
Tsp45I GTSAC 1 cut(s) 734
TspDTI ATGAA 4 cut(s) 326, 446, 765, 1251
TspGWI ACGGA 2 cut(s) 496, 517
VpaK11BI GGWCC 2 cut(s) 405, 680
XapI RAATTY 1 cut(s) 1132
XceI RCATGY 1 cut(s) 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 1082
XmaJI CCTAGG 1 cut(s) 1280
XspI CTAG 3 cut(s) 962, 1190, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.