Rmu_sc0003116.1_g000008

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003116.1
OG0013071
Physical Location & Seq
Reverse (-)
21038 .. 24501
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003116.1_g000008.1.cds

Sequence Viewer

Length: 1326 bp
atggatagtcttcttcttctacttttaggcttccttctcaccctcttttcccaaagattttcgcttccagtagtggtggccttggacctgaaaaacaccacaaggtacaataagcaagtcagcaccttgagacttgagagaattgagaagcacttggacaagatcaacaagcctcctgtcatgactatacagagcccagatggagatatgattgattgtgttcataaaagaaagcagccagctttggatcaccctctgttaaaaaaccacaagatccagaaagtggcaccagaaaggccaaaagtgatgaagacgatgaagccatcatcatcttctcgaagtgagaatgatacagtgaaagcgaagaccggtgggattgcatgggggcaccaaatgtggcatacaaatgggacgagatgtccgagagggactgttccgatacgccggaccacaatgagtgatgttctccgagccaagtctttgtttgactatggcaagaaaagtcaacgacgccccaatattaatacgaataggcaagctgatgcacccgatgtggtcagtgggaatggccatgagcatgcgattgcttacaccggatcatcgaatgaagtatacggtgcaaaagctacgatcaatgtttggaccccagcaatcgaacaaatgaacgagtttagtctttcgcaagtttgggttctgtcgggatcattcgatggttcagatctcaacagcatagaagctggatggcaggtcagtccggagctctatggtgacagcaggcctagattgttcacctattggacgactgattcatatcaggcaactggatgctacaaccttctgtgttctggatttgtgcaaacaaacagtagaatagccattggagcttccatctccccggtctcatcgtatgcaagcgaccaatttgacgttactatcctcatatggaaggatccaaagctaggaaattggtggatgggatatggagagaatagtctagtagggtactggccagcagagctattcacacacttggcagacagagcaacaatggtggaatggggtggtgaggtggtgaatacaaggtccaccggtgagcacacctccacccaaatgggttcgggtcattttgcagaagacgggtttgggaaagcaagctatttccgaaacctagaggttgtagactcagacaatagcctcagttctgtgcgagaagtctcaaccttggccgagaacaccaattgttacaacatcaagagtttctataacaacgattggggcacttacttctactatggagggccaggaaaaaatccaaaatgcccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

441

Amino Acids

49.3

Weight (kDa)

8.56

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 736
AccB1I GGYRCC 2 cut(s) 286, 387
AccB7I CCANNNNNTGG 1 cut(s) 283
AccI GTMKAC 2 cut(s) 612, 1179
AccIII TCCGGA 1 cut(s) 754
AclWI GGATC 6 cut(s) 255, 268, 604, 709, 944, 957
AcoI YGGCCR 3 cut(s) 568, 1007, 1224
AcyI GRCGYC 1 cut(s) 511
AfaI GTAC 2 cut(s) 107, 1004
AfiI CCNNNNNNNGG 2 cut(s) 283, 959
AgeI ACCGGT 2 cut(s) 368, 1088
AhdI GACNNNNNGTC 1 cut(s) 417
AjnI CCWGG 1 cut(s) 1300
AjuI GAANNNNNNNTTGG 2 cut(s) 1125, 1157
AluBI AGCT 9 cut(s) 242, 539, 626, 737, 760, 884, 958, 1018, 1155
AluI AGCT 9 cut(s) 242, 539, 626, 737, 760, 884, 958, 1018, 1155
Alw21I GWGCWC 2 cut(s) 762, 1098
Alw26I GTCTC 3 cut(s) 124, 904, 1219
AlwI GGATC 6 cut(s) 255, 268, 604, 709, 944, 957
Aor13HI TCCGGA 1 cut(s) 754
AoxI GGCC 7 cut(s) 78, 296, 568, 776, 1007, 1224, 1298
ApeKI GCWGC 1 cut(s) 235
AseI ATTAAT 1 cut(s) 522
AsiGI ACCGGT 2 cut(s) 368, 1088
AspS9I GGNCC 5 cut(s) 85, 447, 642, 1083, 1298
AsuC2I CCSGG 1 cut(s) 896
AsuHPI GGTGA 7 cut(s) 31, 242, 779, 781, 1076, 1084, 1103
AvaII GGWCC 4 cut(s) 85, 447, 642, 1083
BaeGI GKGCMC 2 cut(s) 390, 1280
BalI TGGCCA 2 cut(s) 570, 1009
BamHI GGATCC 1 cut(s) 949
BanI GGYRCC 2 cut(s) 286, 387
BanII GRGCYC 2 cut(s) 197, 762
BbsI GAAGAC 4 cut(s) 2, 317, 371, 1140
Bbv12I GWGCWC 2 cut(s) 762, 1098
BbvI GCAGC 1 cut(s) 247
BccI CCATC 6 cut(s) 194, 331, 704, 735, 896, 967
BciT130I CCWGG 1 cut(s) 1302
BcnI CCSGG 1 cut(s) 896
BcoDI GTCTC 3 cut(s) 124, 904, 1219
BfaI CTAG 4 cut(s) 780, 959, 995, 1169
BfuAI ACCTGC 1 cut(s) 736
BglII AGATCT 1 cut(s) 718
BisI GCNGC 1 cut(s) 236
BlsI GCNGC 1 cut(s) 237
Bme1390I CCNGG 2 cut(s) 896, 1302
Bme18I GGWCC 4 cut(s) 85, 447, 642, 1083
BmeRI GACNNNNNGTC 1 cut(s) 417
BmgT120I GGNCC 5 cut(s) 85, 447, 642, 1083, 1298
BmiI GGNNCC 4 cut(s) 288, 389, 644, 951
BmrFI CCNGG 2 cut(s) 896, 1302
BmsI GCATC 2 cut(s) 532, 815
BpiI GAAGAC 4 cut(s) 2, 317, 371, 1140
BplI GAGNNNNNCTC 2 cut(s) 1085, 1117
BpuEI CTTGAG 2 cut(s) 148, 155
BpuMI CCSGG 1 cut(s) 896
BsaBI GATNNNNATC 1 cut(s) 810
BsaHI GRCGYC 1 cut(s) 511
BsaI GGTCTC 1 cut(s) 904
BsaJI CCNNGG 3 cut(s) 81, 894, 1221
BsaWI WCCGGW 4 cut(s) 368, 593, 754, 1088
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 959
Bse118I RCCGGY 2 cut(s) 368, 1088
Bse1I ACTGG 3 cut(s) 68, 826, 1010
Bse8I GATNNNNATC 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 754
BseBI CCWGG 1 cut(s) 1302
BseDI CCNNGG 3 cut(s) 81, 894, 1221
BseGI GGATG 3 cut(s) 746, 830, 978
BseJI GATNNNNATC 1 cut(s) 810
BseLI CCNNNNNNNGG 2 cut(s) 283, 959
BseMII CTCAG 2 cut(s) 1197, 1210
BseNI ACTGG 3 cut(s) 68, 826, 1010
BseSI GKGCMC 2 cut(s) 390, 1280
BseXI GCAGC 1 cut(s) 247
BseYI CCCAGC 1 cut(s) 646
BshFI GGCC 7 cut(s) 80, 298, 570, 778, 1009, 1226, 1300
BshNI GGYRCC 2 cut(s) 286, 387
BshTI ACCGGT 2 cut(s) 368, 1088
BsiHKAI GWGCWC 2 cut(s) 762, 1098
BsiSI CCGG 6 cut(s) 369, 445, 594, 755, 896, 1089
BslFI GGGAC 2 cut(s) 424, 442
BslI CCNNNNNNNGG 2 cut(s) 283, 959
BsmAI GTCTC 3 cut(s) 124, 904, 1219
BsmFI GGGAC 2 cut(s) 424, 442
BsnI GGCC 7 cut(s) 80, 298, 570, 778, 1009, 1226, 1300
Bso31I GGTCTC 1 cut(s) 904
Bsp1286I GDGCHC 5 cut(s) 197, 390, 762, 1098, 1280
Bsp13I TCCGGA 1 cut(s) 754
Bsp143I GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
BspANI GGCC 7 cut(s) 80, 298, 570, 778, 1009, 1226, 1300
BspCNI CTCAG 2 cut(s) 1196, 1209
BspEI TCCGGA 1 cut(s) 754
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 4 cut(s) 288, 389, 644, 951
BspMI ACCTGC 1 cut(s) 736
BspPI GGATC 6 cut(s) 255, 268, 604, 709, 944, 957
BspT107I GGYRCC 2 cut(s) 286, 387
BspTNI GGTCTC 1 cut(s) 904
BsrFI RCCGGY 2 cut(s) 368, 1088
BsrI ACTGG 3 cut(s) 68, 826, 1010
BssAI RCCGGY 2 cut(s) 368, 1088
BssECI CCNNGG 3 cut(s) 81, 894, 1221
BssMI GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
BssNAI GTATAC 1 cut(s) 613
BssNI GRCGYC 1 cut(s) 511
BssT1I CCWWGG 2 cut(s) 81, 1221
Bst1107I GTATAC 1 cut(s) 613
Bst2UI CCWGG 1 cut(s) 1302
Bst4CI ACNGT 4 cut(s) 355, 433, 617, 866
BstACI GRCGYC 1 cut(s) 511
BstC8I GCNNGC 7 cut(s) 240, 537, 579, 776, 913, 1011, 1153
BstDEI CTNAG 2 cut(s) 1183, 1196
BstF5I GGATG 3 cut(s) 746, 830, 978
BstKTI GATC 8 cut(s) 165, 250, 276, 599, 633, 704, 721, 952
BstMAI GTCTC 3 cut(s) 124, 904, 1219
BstMBI GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
BstMWI GCNNNNNNNGC 3 cut(s) 881, 1015, 1040
BstNI CCWGG 1 cut(s) 1302
BstNSI RCATGY 1 cut(s) 581
BstSCI CCNGG 2 cut(s) 894, 1300
BstSLI GKGCMC 2 cut(s) 390, 1280
BstV1I GCAGC 1 cut(s) 247
BstV2I GAAGAC 4 cut(s) 2, 317, 371, 1140
BstX2I RGATCY 3 cut(s) 273, 718, 949
BstXI CCANNNNNNTGG 1 cut(s) 1111
BstYI RGATCY 3 cut(s) 273, 718, 949
BstZ17I GTATAC 1 cut(s) 613
BsuRI GGCC 7 cut(s) 80, 298, 570, 778, 1009, 1226, 1300
BtsCI GGATG 3 cut(s) 746, 830, 978
BtsIMutI CAGTG 2 cut(s) 360, 565
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 7 cut(s) 240, 537, 579, 776, 913, 1011, 1153
CciI TCATGA 1 cut(s) 180
Cfr10I RCCGGY 2 cut(s) 368, 1088
Cfr13I GGNCC 5 cut(s) 85, 447, 642, 1083, 1298
CseI GACGC 1 cut(s) 519
Csp6I GTAC 2 cut(s) 106, 1003
CspAI ACCGGT 2 cut(s) 368, 1088
CspCI CAANNNNNGTGG 2 cut(s) 1032, 1067
CviAII CATG 4 cut(s) 181, 381, 572, 578
CviQI GTAC 2 cut(s) 106, 1003
DdeI CTNAG 2 cut(s) 1183, 1196
DpnI GATC 8 cut(s) 164, 249, 275, 598, 632, 703, 720, 951
DpnII GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
DriI GACNNNNNGTC 1 cut(s) 417
EaeI YGGCCR 3 cut(s) 568, 1007, 1224
Eam1105I GACNNNNNGTC 1 cut(s) 417
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 81, 1221
Eco147I AGGCCT 1 cut(s) 778
Eco24I GRGCYC 2 cut(s) 197, 762
Eco31I GGTCTC 1 cut(s) 904
Eco47I GGWCC 4 cut(s) 85, 447, 642, 1083
Eco53kI GAGCTC 1 cut(s) 760
EcoICRI GAGCTC 1 cut(s) 760
EcoRII CCWGG 1 cut(s) 1300
EcoT14I CCWWGG 2 cut(s) 81, 1221
EcoT38I GRGCYC 2 cut(s) 197, 762
ErhI CCWWGG 2 cut(s) 81, 1221
FaeI CATG 4 cut(s) 184, 384, 575, 581
FaqI GGGAC 2 cut(s) 424, 442
FatI CATG 4 cut(s) 180, 380, 571, 577
FauNDI CATATG 1 cut(s) 941
FblI GTMKAC 2 cut(s) 612, 1179
Fnu4HI GCNGC 1 cut(s) 236
FokI GGATG 3 cut(s) 753, 837, 985
FriOI GRGCYC 2 cut(s) 197, 762
Fsp4HI GCNGC 1 cut(s) 236
FspBI CTAG 4 cut(s) 780, 959, 995, 1169
GluI GCNGC 1 cut(s) 236
GsaI CCCAGC 1 cut(s) 650
HaeIII GGCC 7 cut(s) 80, 298, 570, 778, 1009, 1226, 1300
HapII CCGG 6 cut(s) 369, 445, 594, 755, 896, 1089
HgaI GACGC 1 cut(s) 519
Hin1I GRCGYC 1 cut(s) 511
Hin1II CATG 4 cut(s) 184, 384, 575, 581
HincII GTYRAC 1 cut(s) 506
HindII GTYRAC 1 cut(s) 506
HinfI GANTC 2 cut(s) 806, 1181
HpaII CCGG 6 cut(s) 369, 445, 594, 755, 896, 1089
HphI GGTGA 7 cut(s) 31, 242, 779, 781, 1076, 1084, 1103
Hpy166II GTNNAC 5 cut(s) 506, 613, 789, 1086, 1180
Hpy188I TCNGA 6 cut(s) 423, 438, 470, 718, 1163, 1186
Hpy188III TCNNGA 7 cut(s) 181, 277, 336, 699, 755, 846, 1252
Hpy8I GTNNAC 5 cut(s) 506, 613, 789, 1086, 1180
Hpy99I CGWCG 1 cut(s) 513
HpyAV CCTTC 3 cut(s) 44, 845, 940
HpyCH4III ACNGT 4 cut(s) 355, 433, 617, 866
HpyCH4IV ACGT 1 cut(s) 927
HpyCH4V TGCA 6 cut(s) 380, 545, 620, 856, 911, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 881, 1015, 1040
HpyF3I CTNAG 2 cut(s) 1183, 1196
HpySE526I ACGT 1 cut(s) 927
Hsp92I GRCGYC 1 cut(s) 511
Hsp92II CATG 4 cut(s) 184, 384, 575, 581
Kpn2I TCCGGA 1 cut(s) 754
Kzo9I GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
LmnI GCTCC 2 cut(s) 757, 881
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 2 cut(s) 532, 815
MaeI CTAG 4 cut(s) 780, 959, 995, 1169
MaeII ACGT 1 cut(s) 927
MaeIII GTNAC 3 cut(s) 767, 928, 1241
MalI GATC 8 cut(s) 164, 249, 275, 598, 632, 703, 720, 951
MboI GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
MboII GAAGA 6 cut(s) 5, 8, 322, 324, 376, 1145
MfeI CAATTG 1 cut(s) 1237
MflI RGATCY 3 cut(s) 273, 718, 949
MhlI GDGCHC 5 cut(s) 197, 390, 762, 1098, 1280
MlsI TGGCCA 2 cut(s) 570, 1009
MluCI AATT 4 cut(s) 141, 920, 964, 1237
MluNI TGGCCA 2 cut(s) 570, 1009
MlyI GAGTC 1 cut(s) 1175
Mox20I TGGCCA 2 cut(s) 570, 1009
MroI TCCGGA 1 cut(s) 754
MscI TGGCCA 2 cut(s) 570, 1009
MseI TTAA 2 cut(s) 260, 522
MslI CAYNNNNRTG 3 cut(s) 405, 576, 1109
Msp20I TGGCCA 2 cut(s) 570, 1009
MspI CCGG 6 cut(s) 369, 445, 594, 755, 896, 1089
MspR9I CCNGG 2 cut(s) 896, 1302
MunI CAATTG 1 cut(s) 1237
MvaI CCWGG 1 cut(s) 1302
MwoI GCNNNNNNNGC 3 cut(s) 881, 1015, 1040
NciI CCSGG 1 cut(s) 896
NdeI CATATG 1 cut(s) 941
NdeII GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
NlaIII CATG 4 cut(s) 184, 384, 575, 581
NlaIV GGNNCC 4 cut(s) 288, 389, 644, 951
NmeAIII GCCGAG 1 cut(s) 1252
NmuCI GTSAC 1 cut(s) 767
NspI RCATGY 1 cut(s) 581
PaeI GCATGC 1 cut(s) 581
PagI TCATGA 1 cut(s) 180
PceI AGGCCT 1 cut(s) 778
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PfeI GAWTC 1 cut(s) 806
PflMI CCANNNNNTGG 1 cut(s) 283
PinAI ACCGGT 2 cut(s) 368, 1088
PkrI GCNGC 1 cut(s) 237
PleI GAGTC 1 cut(s) 1175
PpsI GAGTC 1 cut(s) 1175
PshBI ATTAAT 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 1300
PspFI CCCAGC 1 cut(s) 646
PspGI CCWGG 1 cut(s) 1300
PspN4I GGNNCC 4 cut(s) 288, 389, 644, 951
PspPI GGNCC 5 cut(s) 85, 447, 642, 1083, 1298
PsuI RGATCY 3 cut(s) 273, 718, 949
RsaI GTAC 2 cut(s) 107, 1004
RsaNI GTAC 2 cut(s) 106, 1003
RseI CAYNNNNRTG 3 cut(s) 405, 576, 1109
SacI GAGCTC 1 cut(s) 762
SaqAI TTAA 2 cut(s) 260, 522
SatI GCNGC 1 cut(s) 236
Sau3AI GATC 8 cut(s) 162, 247, 273, 596, 630, 701, 718, 949
Sau96I GGNCC 5 cut(s) 85, 447, 642, 1083, 1298
SchI GAGTC 1 cut(s) 1175
ScrFI CCNGG 2 cut(s) 896, 1302
SduI GDGCHC 5 cut(s) 197, 390, 762, 1098, 1280
SfaNI GCATC 2 cut(s) 532, 815
SgrAI CRCCGGYG 1 cut(s) 1088
SinI GGWCC 4 cut(s) 85, 447, 642, 1083
SmiMI CAYNNNNRTG 3 cut(s) 405, 576, 1109
SmlI CTYRAG 2 cut(s) 127, 134
SmoI CTYRAG 2 cut(s) 127, 134
SphI GCATGC 1 cut(s) 581
Sse9I AATT 4 cut(s) 141, 920, 964, 1237
SseBI AGGCCT 1 cut(s) 778
SspI AATATT 1 cut(s) 520
SspMI CTAG 4 cut(s) 780, 959, 995, 1169
SstI GAGCTC 1 cut(s) 762
StuI AGGCCT 1 cut(s) 778
StyD4I CCNGG 2 cut(s) 894, 1300
StyI CCWWGG 2 cut(s) 81, 1221
TaaI ACNGT 4 cut(s) 355, 433, 617, 866
TaiI ACGT 1 cut(s) 930
TaqI TCGA 4 cut(s) 337, 602, 654, 708
TasI AATT 4 cut(s) 141, 920, 964, 1237
TfiI GAWTC 1 cut(s) 806
Tru1I TTAA 2 cut(s) 260, 522
Tru9I TTAA 2 cut(s) 260, 522
TscAI CASTG 2 cut(s) 360, 565
TseFI GTSAC 1 cut(s) 767
TseI GCWGC 1 cut(s) 235
Tsp45I GTSAC 1 cut(s) 767
TspDTI ATGAA 6 cut(s) 212, 323, 332, 621, 677, 798
TspRI CASTG 2 cut(s) 360, 565
Van91I CCANNNNNTGG 1 cut(s) 283
VpaK11BI GGWCC 4 cut(s) 85, 447, 642, 1083
VspI ATTAAT 1 cut(s) 522
XceI RCATGY 1 cut(s) 581
XmiI GTMKAC 2 cut(s) 612, 1179
XspI CTAG 4 cut(s) 780, 959, 995, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.