Prupe.6G353500_v2.0.a1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0013071
Physical Location & Seq
Reverse (-)
30055887 .. 30060498
4612 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G353500.1

Sequence Viewer

Length: 1458 bp
ATGATGATGATGATAGCTACTTCTACTATTAATATTGGTGGAAGGAAGAAGAGAGAGCAGTACTTGTTTCTCATCATCTTCTTATGCATCTGCACCATCTTTTTCCAAAGGTTTTCACTTTCAGTGGAAGCTGCTTCGAATTCGGGGAGCCTCAAATACACAAGGCATAATTATAAGCAAGTTAGCAGCTTGAGACTGCAAAGGATTGAGAAGCACCTCAACAAGATCAACAAGCCTGCTGTCATCACCGTTCAGAGCCCAGATGGAGATATAATAGATTGCGTTCATAAAAGAAAACAGCCAGCTTTGGATCACCCTCTCTTAAAGAATCACAAGATCCAGAAGGAGGCACCAGAAAGGCCAAAAGCGATGAAGAAGAAGATGAAGCACACAAATGATAAAGATGAAGAAGGGATTAACAATAATAATGGAGATGAAGCAGCAGCGGCAGTTGAGGGTAAGGGTAAGGAGGGTGCATGGGTTGTGCACCAAACGTGGCACAGAAATGGGACGAGATGTCCCAGAGGAACGGTTCCAATACGGCGAAGCACAGTGGATGATGTCCTGCGGGCCAAATCTTTGTTTGACTATGGCAAGAAGAAACAGCAGCAACCTCCTAATAATTTCAATTTTAATAATAATCTCACTGCTGATCTTTCTGCTAGGCGCACCAATGCACCCGACGTAGTTAGCGGCAATGGCCATGAGCATGCAATCGCGTACACCGGATCATTGCAAGAAGTGTATGGGGCAAGGGCGACAATCAATGTGTGGACCCCCTCGATCGAACAAGCAAACGAGTTCAGTCTCTCCCAGATTTGGGTTCTTTCCGGATCATTCGACGGCTCAGATCTCAACAGCATAGAAGCTGGATGGCAGGTCAGTCCGGAGCTCTATGGTGACAACAGTCCCAGATTGTTCACTTACTGGACGACTGATTCATATCAGGCGACTGGATGCTACAATCTTCTGTGTTCCGGGTTTATCCAAACAAACAGTAGGATAGCAATTGGAGCTTCCATTTCTCCAGTCTCATCCTATGCCAGCAACCAATACGATGTCACCATTCTCATATGGAAGGATCCAAAGCTAGGGAATTGGTGGATGGGATTTGGAGACAATACACTTGTAGGGTACTGGCCAGCGGATTTATTCACACACTTGGCAGAGAAGGCGACGATGGTAGAATGGGGTGGCGAGGTGGTGAACACAAGAGCCAACGGTGAGCACACCGCCACGCAAATGGGTTCGGGTCATTTCGCTGAAGACGGGTTTGGGAAAGCAAGCTATTTCCGAAACCTTGAGATCGTGGACTCGGACAACAGCCTCAGCTCGGTCCCAGGTGTGTCAACTTTGGCTGAGAACACCAATTGCTACAACATCGAGAGCTCGTACAACAATGAATGGGGCACGCACTTCTACTATGGCGGCCCTGGCAAAAATTCACGATGCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

486

Amino Acids

54.12

Weight (kDa)

8.27

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 868
AccB1I GGYRCC 1 cut(s) 349
AccII CGCG 1 cut(s) 719
AccIII TCCGGA 2 cut(s) 830, 886
AciI CCGC 6 cut(s) 446, 568, 693, 1145, 1233, 1428
AclWI GGATC 6 cut(s) 318, 331, 736, 841, 1076, 1089
AcoI YGGCCR 2 cut(s) 700, 1139
AcsI RAATTY 2 cut(s) 139, 1441
AcuI CTGAAG 1 cut(s) 1284
AfaI GTAC 4 cut(s) 62, 722, 1136, 1394
AfiI CCNNNNNNNGG 5 cut(s) 346, 819, 820, 1091, 1333
AgsI TTSAA 1 cut(s) 628
AhdI GACNNNNNGTC 1 cut(s) 516
AjnI CCWGG 2 cut(s) 1339, 1432
AjuI GAANNNNNNNTTGG 2 cut(s) 1257, 1289
Alw21I GWGCWC 4 cut(s) 489, 894, 1230, 1391
Alw26I GTCTC 4 cut(s) 187, 812, 1036, 1110
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 6 cut(s) 318, 331, 736, 841, 1076, 1089
Aor13HI TCCGGA 2 cut(s) 830, 886
AoxI GGCC 5 cut(s) 359, 570, 700, 1139, 1429
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 5 cut(s) 131, 186, 440, 443, 607
ApoI RAATTY 2 cut(s) 139, 1441
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 1 cut(s) 669
AspS9I GGNCC 4 cut(s) 570, 774, 1336, 1430
AsuC2I CCSGG 1 cut(s) 979
AsuHPI GGTGA 6 cut(s) 238, 305, 911, 1054, 1216, 1235
AsuII TTCGAA 1 cut(s) 137
AvaII GGWCC 2 cut(s) 774, 1336
BaeGI GKGCMC 2 cut(s) 489, 1412
BalI TGGCCA 2 cut(s) 702, 1141
BamHI GGATCC 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 349
BanII GRGCYC 3 cut(s) 260, 894, 1391
BbsI GAAGAC 1 cut(s) 1272
Bbv12I GWGCWC 4 cut(s) 489, 894, 1230, 1391
BbvCI CCTCAGC 1 cut(s) 1328
BbvI GCAGC 5 cut(s) 118, 198, 452, 455, 619
BccI CCATC 5 cut(s) 104, 257, 867, 1099, 1174
BceAI ACGGC 2 cut(s) 557, 859
BciT130I CCWGG 2 cut(s) 1341, 1434
BcnI CCSGG 1 cut(s) 979
BcoDI GTCTC 4 cut(s) 187, 812, 1036, 1110
BfaI CTAG 2 cut(s) 663, 1091
BfuAI ACCTGC 1 cut(s) 868
BglII AGATCT 1 cut(s) 850
BisI GCNGC 8 cut(s) 132, 187, 441, 444, 447, 608, 694, 1429
BlsI GCNGC 8 cut(s) 133, 188, 442, 445, 448, 609, 695, 1430
BmcAI AGTACT 1 cut(s) 62
Bme1390I CCNGG 3 cut(s) 979, 1341, 1434
Bme18I GGWCC 2 cut(s) 774, 1336
BmeRI GACNNNNNGTC 1 cut(s) 516
BmgT120I GGNCC 4 cut(s) 570, 774, 1336, 1430
BmiI GGNNCC 6 cut(s) 149, 351, 534, 776, 1083, 1338
BmrFI CCNGG 3 cut(s) 979, 1341, 1434
BmsI GCATC 3 cut(s) 96, 947, 1439
BoxI GACNNNNGTC 1 cut(s) 906
BpiI GAAGAC 1 cut(s) 1272
BpmI CTGGAG 1 cut(s) 1011
Bpu10I CCTNAGC 1 cut(s) 1328
Bpu14I TTCGAA 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 211, 1322
BpuMI CCSGG 1 cut(s) 979
BsaBI GATNNNNATC 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 1339, 1432
BsaWI WCCGGW 3 cut(s) 725, 830, 886
Bsc4I CCNNNNNNNGG 5 cut(s) 346, 819, 820, 1091, 1333
Bse1I ACTGG 4 cut(s) 932, 958, 1028, 1142
Bse3DI GCAATG 2 cut(s) 703, 731
Bse8I GATNNNNATC 1 cut(s) 942
BseAI TCCGGA 2 cut(s) 830, 886
BseBI CCWGG 2 cut(s) 1341, 1434
BseDI CCNNGG 2 cut(s) 1339, 1432
BseGI GGATG 5 cut(s) 562, 878, 962, 1034, 1110
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 5 cut(s) 346, 819, 820, 1091, 1333
BseMI GCAATG 2 cut(s) 703, 731
BseMII CTCAG 3 cut(s) 861, 1342, 1350
BseNI ACTGG 4 cut(s) 932, 958, 1028, 1142
BseSI GKGCMC 2 cut(s) 489, 1412
BseXI GCAGC 5 cut(s) 118, 198, 452, 455, 619
BsgI GTGCAG 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 719
Bsh1285I CGRYCG 1 cut(s) 786
BshFI GGCC 5 cut(s) 361, 572, 702, 1141, 1431
BshNI GGYRCC 1 cut(s) 349
BsiEI CGRYCG 1 cut(s) 786
BsiHKAI GWGCWC 4 cut(s) 489, 894, 1230, 1391
BsiSI CCGG 4 cut(s) 726, 831, 887, 978
BslFI GGGAC 4 cut(s) 504, 523, 894, 1322
BslI CCNNNNNNNGG 5 cut(s) 346, 819, 820, 1091, 1333
BsmAI GTCTC 4 cut(s) 187, 812, 1036, 1110
BsmFI GGGAC 4 cut(s) 504, 523, 894, 1322
BsnI GGCC 5 cut(s) 361, 572, 702, 1141, 1431
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 6 cut(s) 260, 489, 894, 1230, 1391, 1412
Bsp13I TCCGGA 2 cut(s) 830, 886
BspACI CCGC 6 cut(s) 446, 568, 693, 1145, 1233, 1428
BspANI GGCC 5 cut(s) 361, 572, 702, 1141, 1431
BspCNI CTCAG 3 cut(s) 860, 1341, 1351
BspEI TCCGGA 2 cut(s) 830, 886
BspFNI CGCG 1 cut(s) 719
BspLI GGNNCC 6 cut(s) 149, 351, 534, 776, 1083, 1338
BspMI ACCTGC 1 cut(s) 868
BspPI GGATC 6 cut(s) 318, 331, 736, 841, 1076, 1089
BspT104I TTCGAA 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 703, 731
BsrI ACTGG 4 cut(s) 932, 958, 1028, 1142
BssECI CCNNGG 2 cut(s) 1339, 1432
Bst2UI CCWGG 2 cut(s) 1341, 1434
Bst4CI ACNGT 6 cut(s) 250, 532, 553, 908, 998, 1223
Bst6I CTCTTC 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 8 cut(s) 237, 303, 570, 711, 1045, 1143, 1285, 1412
BstDEI CTNAG 3 cut(s) 847, 1328, 1359
BstF5I GGATG 5 cut(s) 562, 878, 962, 1034, 1110
BstFNI CGCG 1 cut(s) 719
BstHHI GCGC 1 cut(s) 669
BstMAI GTCTC 4 cut(s) 187, 812, 1036, 1110
BstMCI CGRYCG 1 cut(s) 786
BstMWI GCNNNNNNNGC 5 cut(s) 446, 699, 1013, 1172, 1434
BstNI CCWGG 2 cut(s) 1341, 1434
BstNSI RCATGY 1 cut(s) 713
BstPAI GACNNNNGTC 1 cut(s) 906
BstSCI CCNGG 3 cut(s) 977, 1339, 1432
BstSLI GKGCMC 2 cut(s) 489, 1412
BstUI CGCG 1 cut(s) 719
BstV1I GCAGC 5 cut(s) 118, 198, 452, 455, 619
BstV2I GAAGAC 1 cut(s) 1272
BstX2I RGATCY 3 cut(s) 336, 850, 1081
BstXI CCANNNNNNTGG 1 cut(s) 1243
BstYI RGATCY 3 cut(s) 336, 850, 1081
BsuRI GGCC 5 cut(s) 361, 572, 702, 1141, 1431
BtgZI GCGATG 1 cut(s) 383
BtsCI GGATG 5 cut(s) 562, 878, 962, 1034, 1110
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 3 cut(s) 129, 558, 645
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 8 cut(s) 237, 303, 570, 711, 1045, 1143, 1285, 1412
CfoI GCGC 1 cut(s) 669
Cfr13I GGNCC 4 cut(s) 570, 774, 1336, 1430
Csp6I GTAC 4 cut(s) 61, 721, 1135, 1393
CviAII CATG 3 cut(s) 477, 704, 710
CviQI GTAC 4 cut(s) 61, 721, 1135, 1393
DdeI CTNAG 3 cut(s) 847, 1328, 1359
DriI GACNNNNNGTC 1 cut(s) 516
EaeI YGGCCR 2 cut(s) 700, 1139
Eam1104I CTCTTC 1 cut(s) 44
Eam1105I GACNNNNNGTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 44
Ecl136II GAGCTC 2 cut(s) 892, 1389
Eco24I GRGCYC 3 cut(s) 260, 894, 1391
Eco47I GGWCC 2 cut(s) 774, 1336
Eco53kI GAGCTC 2 cut(s) 892, 1389
Eco57I CTGAAG 1 cut(s) 1284
EcoICRI GAGCTC 2 cut(s) 892, 1389
EcoRI GAATTC 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 1339, 1432
EcoT22I ATGCAT 1 cut(s) 89
EcoT38I GRGCYC 3 cut(s) 260, 894, 1391
FaeI CATG 3 cut(s) 480, 707, 713
FaqI GGGAC 4 cut(s) 504, 523, 894, 1322
FatI CATG 3 cut(s) 476, 703, 709
FauI CCCGC 1 cut(s) 561
FauNDI CATATG 1 cut(s) 1073
Fnu4HI GCNGC 8 cut(s) 132, 187, 441, 444, 447, 608, 694, 1429
FokI GGATG 5 cut(s) 569, 885, 969, 1021, 1117
FriOI GRGCYC 3 cut(s) 260, 894, 1391
Fsp4HI GCNGC 8 cut(s) 132, 187, 441, 444, 447, 608, 694, 1429
FspBI CTAG 2 cut(s) 663, 1091
GlaI GCGC 1 cut(s) 668
GluI GCNGC 8 cut(s) 132, 187, 441, 444, 447, 608, 694, 1429
GsuI CTGGAG 1 cut(s) 1011
HaeIII GGCC 5 cut(s) 361, 572, 702, 1141, 1431
HapII CCGG 4 cut(s) 726, 831, 887, 978
HhaI GCGC 1 cut(s) 669
Hin1II CATG 3 cut(s) 480, 707, 713
Hin6I GCGC 1 cut(s) 667
HinP1I GCGC 1 cut(s) 667
HincII GTYRAC 1 cut(s) 1350
HindII GTYRAC 1 cut(s) 1350
HinfI GANTC 3 cut(s) 328, 938, 1313
HpaII CCGG 4 cut(s) 726, 831, 887, 978
HphI GGTGA 6 cut(s) 238, 305, 911, 1054, 1216, 1235
Hpy166II GTNNAC 7 cut(s) 487, 723, 774, 921, 1207, 1312, 1350
Hpy188I TCNGA 4 cut(s) 255, 850, 1295, 1318
Hpy188III TCNNGA 5 cut(s) 340, 831, 887, 1384, 1446
Hpy8I GTNNAC 7 cut(s) 487, 723, 774, 921, 1207, 1312, 1350
Hpy99I CGWCG 3 cut(s) 686, 845, 1180
HpyAV CCTTC 5 cut(s) 36, 337, 404, 1072, 1165
HpyCH4III ACNGT 6 cut(s) 250, 532, 553, 908, 998, 1223
HpyCH4IV ACGT 2 cut(s) 494, 684
HpyCH4V TGCA 8 cut(s) 87, 93, 199, 476, 487, 677, 713, 736
HpyF10VI GCNNNNNNNGC 5 cut(s) 446, 699, 1013, 1172, 1434
HpyF3I CTNAG 3 cut(s) 847, 1328, 1359
HpySE526I ACGT 2 cut(s) 494, 684
Hsp92II CATG 3 cut(s) 480, 707, 713
HspAI GCGC 1 cut(s) 667
Kpn2I TCCGGA 2 cut(s) 830, 886
LmnI GCTCC 3 cut(s) 147, 889, 1013
Lsp1109I GCAGC 5 cut(s) 118, 198, 452, 455, 619
LweI GCATC 3 cut(s) 96, 947, 1439
MaeI CTAG 2 cut(s) 663, 1091
MaeII ACGT 2 cut(s) 494, 684
MaeIII GTNAC 2 cut(s) 899, 1060
MfeI CAATTG 2 cut(s) 1008, 1369
MflI RGATCY 3 cut(s) 336, 850, 1081
MhlI GDGCHC 6 cut(s) 260, 489, 894, 1230, 1391, 1412
MlsI TGGCCA 2 cut(s) 702, 1141
MluCI AATT 8 cut(s) 139, 169, 622, 628, 1008, 1096, 1369, 1441
MluNI TGGCCA 2 cut(s) 702, 1141
MlyI GAGTC 1 cut(s) 1307
Mox20I TGGCCA 2 cut(s) 702, 1141
Mph1103I ATGCAT 1 cut(s) 89
MroI TCCGGA 2 cut(s) 830, 886
MscI TGGCCA 2 cut(s) 702, 1141
MseI TTAA 5 cut(s) 30, 323, 417, 633, 1456
MslI CAYNNNNRTG 4 cut(s) 393, 504, 708, 1241
Msp20I TGGCCA 2 cut(s) 702, 1141
MspA1I CMGCKG 2 cut(s) 446, 1145
MspI CCGG 4 cut(s) 726, 831, 887, 978
MspR9I CCNGG 3 cut(s) 979, 1341, 1434
MunI CAATTG 2 cut(s) 1008, 1369
MvaI CCWGG 2 cut(s) 1341, 1434
MvnI CGCG 1 cut(s) 719
MwoI GCNNNNNNNGC 5 cut(s) 446, 699, 1013, 1172, 1434
NciI CCSGG 1 cut(s) 979
NdeI CATATG 1 cut(s) 1073
NlaIII CATG 3 cut(s) 480, 707, 713
NlaIV GGNNCC 6 cut(s) 149, 351, 534, 776, 1083, 1338
NmuCI GTSAC 2 cut(s) 899, 1060
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 713
NspV TTCGAA 1 cut(s) 137
PaeI GCATGC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 328, 938
PkrI GCNGC 8 cut(s) 133, 188, 442, 445, 448, 609, 695, 1430
Ple19I CGATCG 1 cut(s) 786
PleI GAGTC 1 cut(s) 1307
PpsI GAGTC 1 cut(s) 1307
PshAI GACNNNNGTC 1 cut(s) 906
PshBI ATTAAT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 174
Psp124BI GAGCTC 2 cut(s) 894, 1391
Psp6I CCWGG 2 cut(s) 1339, 1432
PspGI CCWGG 2 cut(s) 1339, 1432
PspN4I GGNNCC 6 cut(s) 149, 351, 534, 776, 1083, 1338
PspPI GGNCC 4 cut(s) 570, 774, 1336, 1430
PsuI RGATCY 3 cut(s) 336, 850, 1081
PvuI CGATCG 1 cut(s) 786
RsaI GTAC 4 cut(s) 62, 722, 1136, 1394
RsaNI GTAC 4 cut(s) 61, 721, 1135, 1393
RseI CAYNNNNRTG 4 cut(s) 393, 504, 708, 1241
SacI GAGCTC 2 cut(s) 894, 1391
SaqAI TTAA 5 cut(s) 30, 323, 417, 633, 1456
SatI GCNGC 8 cut(s) 132, 187, 441, 444, 447, 608, 694, 1429
Sau96I GGNCC 4 cut(s) 570, 774, 1336, 1430
ScaI AGTACT 1 cut(s) 62
SchI GAGTC 1 cut(s) 1307
ScrFI CCNGG 3 cut(s) 979, 1341, 1434
SduI GDGCHC 6 cut(s) 260, 489, 894, 1230, 1391, 1412
SfaNI GCATC 3 cut(s) 96, 947, 1439
SfuI TTCGAA 1 cut(s) 137
SinI GGWCC 2 cut(s) 774, 1336
SmiMI CAYNNNNRTG 4 cut(s) 393, 504, 708, 1241
SmlI CTYRAG 2 cut(s) 190, 1301
SmoI CTYRAG 2 cut(s) 190, 1301
SphI GCATGC 1 cut(s) 713
Sse9I AATT 8 cut(s) 139, 169, 622, 628, 1008, 1096, 1369, 1441
SsiI CCGC 6 cut(s) 446, 568, 693, 1145, 1233, 1428
SspI AATATT 1 cut(s) 34
SspMI CTAG 2 cut(s) 663, 1091
SstI GAGCTC 2 cut(s) 894, 1391
StyD4I CCNGG 3 cut(s) 977, 1339, 1432
TaaI ACNGT 6 cut(s) 250, 532, 553, 908, 998, 1223
TaiI ACGT 2 cut(s) 497, 687
TaqI TCGA 5 cut(s) 137, 782, 786, 840, 1383
TaqII GACCGA 1 cut(s) 1324
TasI AATT 8 cut(s) 139, 169, 622, 628, 1008, 1096, 1369, 1441
TatI WGTACW 1 cut(s) 60
TauI GCSGC 3 cut(s) 449, 696, 1431
TfiI GAWTC 2 cut(s) 328, 938
Tru1I TTAA 5 cut(s) 30, 323, 417, 633, 1456
Tru9I TTAA 5 cut(s) 30, 323, 417, 633, 1456
TscAI CASTG 3 cut(s) 129, 558, 652
TseFI GTSAC 2 cut(s) 899, 1060
TseI GCWGC 5 cut(s) 131, 186, 440, 443, 607
Tsp45I GTSAC 2 cut(s) 899, 1060
TspDTI ATGAA 7 cut(s) 275, 386, 398, 420, 450, 930, 1416
TspRI CASTG 3 cut(s) 129, 558, 652
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 774, 1336
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 2 cut(s) 139, 1441
XceI RCATGY 1 cut(s) 713
XspI CTAG 2 cut(s) 663, 1091
ZrmI AGTACT 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.