Rw3G001210

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013071
Physical Location & Seq
Forward (+)
997394 .. 1000555
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001210.1

Sequence Viewer

Length: 1119 bp
ATGATTGATTGTGTTCATAAAAGAAAGCAGCCAGCTTTGGATCACCCTCTGTTAAAAAACCACAAGATCCAGAAAGTGGCACCAGAAAGGCCAAAAGTGATGAAGACGATGAAGCCATCATCATCTTCTCGAAGTGAGAATGATACAGTGAAAGCGAAGACCAGTGGGATTGCATGGGGGCACCAAATGTGGCATACAAATGGGACGAGATGTCCGAGAGGGACTGTTCCGATACGCCGGACCACAATGAGTGATGTTCTCCGAGCCAAGTCTTTGTTTGACTATGGCAAGAAAAGTCAACGACGCACCAATATTAATACGAATAGGCAAGCTGATGCACCCGATGTGGTCAGTGGGAATGGCCATGAGCATGCGATTGCTTACACCGGATCATCGAATGAAGTATACGGTGCAAAAGCTACGATCAATGTTTGGACCCCAGCAATCGAACAAATGAACGAGTTTAGTCTTTCGCAAGTTTGGGTTCTGTCGGGATCATTCGATGGTTCAGATCTCAACAGCATAGAAGCTGGATGGCAGGTCAGTCCGGAGCTCTATGGTGACAGCAGGCCTAGATTGTTCACCTATTGGACGACTGATTCATATCAGGCAACTGGATGCTACAACCTTCTGTGTTCTGGATTTGTGCAAACAAACAGTAGAATAGCCATTGGAGCTTCCATCTCCCCGGTCTCATCGTATGCAAGCGACCAATTTGACGTTACTATCCTCATATGGAAGGATCCAAAGCTAGGAAATTGGTGGATGGGATACGGAGAGAATAGTCTAGTAGGGTACTGGCCAGCAGAGCTATTCACACACTTGGCAGACAGAGCAACAATGGTGGAATGGGGTGGTGAGGTGGTGAATACAAGGTCCACCGGTGAGCACACCTCCACCCAAATGGGATCGGGTCATTTTGCAGAAGACGGGTTTGGGAAAGCAAGCTATTTCCGAAACCTAGAGGTTGTAGACTCAGACAATAGCCTCAGTTCTGTGCGAGAAGTCTCAACCTTGGCCGAGAACACCAATTGTTACAACATCAAGAGCTTCTATAACAACGATTGGGGCACTTACTTCTACTATGGAGGGCCAGGAAGAAATCCAAAATGCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

372

Amino Acids

41.48

Weight (kDa)

8.12

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 1 - 128 8.1e-37 Neprosin activation peptide
Neprosin PF03080 153 - 365 2e-88 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 2 cut(s) 79, 180
AccB7I CCANNNNNTGG 1 cut(s) 76
AccI GTMKAC 2 cut(s) 405, 972
AccIII TCCGGA 1 cut(s) 547
AclWI GGATC 7 cut(s) 48, 61, 397, 502, 737, 750, 916
AcoI YGGCCR 3 cut(s) 361, 800, 1017
AfaI GTAC 1 cut(s) 797
AfiI CCNNNNNNNGG 2 cut(s) 76, 752
AgeI ACCGGT 1 cut(s) 881
AhdI GACNNNNNGTC 1 cut(s) 210
AjnI CCWGG 1 cut(s) 1093
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
Alw21I GWGCWC 2 cut(s) 555, 891
Alw26I GTCTC 2 cut(s) 697, 1012
AlwI GGATC 7 cut(s) 48, 61, 397, 502, 737, 750, 916
Aor13HI TCCGGA 1 cut(s) 547
AoxI GGCC 6 cut(s) 89, 361, 569, 800, 1017, 1091
ApeKI GCWGC 1 cut(s) 28
AseI ATTAAT 1 cut(s) 315
AsiGI ACCGGT 1 cut(s) 881
AspS9I GGNCC 4 cut(s) 240, 435, 876, 1091
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 6 cut(s) 35, 572, 574, 869, 877, 896
AvaII GGWCC 3 cut(s) 240, 435, 876
BaeGI GKGCMC 2 cut(s) 183, 1073
BalI TGGCCA 2 cut(s) 363, 802
BamHI GGATCC 1 cut(s) 742
BanI GGYRCC 2 cut(s) 79, 180
BanII GRGCYC 1 cut(s) 555
BbsI GAAGAC 3 cut(s) 110, 164, 933
Bbv12I GWGCWC 2 cut(s) 555, 891
BbvI GCAGC 1 cut(s) 40
BccI CCATC 5 cut(s) 124, 497, 528, 689, 760
BciT130I CCWGG 1 cut(s) 1095
BciVI GTATCC 1 cut(s) 764
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 697, 1012
BfaI CTAG 4 cut(s) 573, 752, 788, 962
BfuAI ACCTGC 1 cut(s) 529
BfuI GTATCC 1 cut(s) 764
BglII AGATCT 1 cut(s) 511
BisI GCNGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 30
Bme1390I CCNGG 2 cut(s) 689, 1095
Bme18I GGWCC 3 cut(s) 240, 435, 876
BmeRI GACNNNNNGTC 1 cut(s) 210
BmgT120I GGNCC 4 cut(s) 240, 435, 876, 1091
BmiI GGNNCC 4 cut(s) 81, 182, 437, 744
BmrFI CCNGG 2 cut(s) 689, 1095
BmsI GCATC 2 cut(s) 325, 608
BpiI GAAGAC 3 cut(s) 110, 164, 933
BplI GAGNNNNNCTC 2 cut(s) 878, 910
BpuMI CCSGG 1 cut(s) 689
BsaBI GATNNNNATC 1 cut(s) 603
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 2 cut(s) 687, 1014
BsaWI WCCGGW 3 cut(s) 386, 547, 881
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 752
Bse118I RCCGGY 1 cut(s) 881
Bse1I ACTGG 3 cut(s) 162, 619, 803
Bse8I GATNNNNATC 1 cut(s) 603
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 1 cut(s) 1095
BseDI CCNNGG 2 cut(s) 687, 1014
BseGI GGATG 3 cut(s) 539, 623, 771
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 2 cut(s) 76, 752
BseMII CTCAG 2 cut(s) 990, 1003
BseNI ACTGG 3 cut(s) 162, 619, 803
BseSI GKGCMC 2 cut(s) 183, 1073
BseXI GCAGC 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 439
BshFI GGCC 6 cut(s) 91, 363, 571, 802, 1019, 1093
BshNI GGYRCC 2 cut(s) 79, 180
BshTI ACCGGT 1 cut(s) 881
BsiHKAI GWGCWC 2 cut(s) 555, 891
BsiSI CCGG 5 cut(s) 238, 387, 548, 689, 882
BslFI GGGAC 2 cut(s) 217, 235
BslI CCNNNNNNNGG 2 cut(s) 76, 752
BsmAI GTCTC 2 cut(s) 697, 1012
BsmFI GGGAC 2 cut(s) 217, 235
BsnI GGCC 6 cut(s) 91, 363, 571, 802, 1019, 1093
Bso31I GGTCTC 1 cut(s) 697
Bsp1286I GDGCHC 4 cut(s) 183, 555, 891, 1073
Bsp13I TCCGGA 1 cut(s) 547
Bsp143I GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
BspANI GGCC 6 cut(s) 91, 363, 571, 802, 1019, 1093
BspCNI CTCAG 2 cut(s) 989, 1002
BspEI TCCGGA 1 cut(s) 547
BspLI GGNNCC 4 cut(s) 81, 182, 437, 744
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 7 cut(s) 48, 61, 397, 502, 737, 750, 916
BspT107I GGYRCC 2 cut(s) 79, 180
BspTNI GGTCTC 1 cut(s) 697
BsrFI RCCGGY 1 cut(s) 881
BsrI ACTGG 3 cut(s) 162, 619, 803
BssAI RCCGGY 1 cut(s) 881
BssECI CCNNGG 2 cut(s) 687, 1014
BssMI GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
BssNAI GTATAC 1 cut(s) 406
BssT1I CCWWGG 1 cut(s) 1014
Bst1107I GTATAC 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 1095
Bst4CI ACNGT 4 cut(s) 148, 226, 410, 659
BstC8I GCNNGC 7 cut(s) 33, 330, 372, 569, 706, 804, 946
BstDEI CTNAG 2 cut(s) 976, 989
BstF5I GGATG 3 cut(s) 539, 623, 771
BstKTI GATC 8 cut(s) 43, 69, 392, 426, 497, 514, 745, 911
BstMAI GTCTC 2 cut(s) 697, 1012
BstMBI GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
BstMWI GCNNNNNNNGC 3 cut(s) 674, 808, 833
BstNI CCWGG 1 cut(s) 1095
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 687, 1093
BstSLI GKGCMC 2 cut(s) 183, 1073
BstV1I GCAGC 1 cut(s) 40
BstV2I GAAGAC 3 cut(s) 110, 164, 933
BstX2I RGATCY 3 cut(s) 66, 511, 742
BstXI CCANNNNNNTGG 1 cut(s) 904
BstYI RGATCY 3 cut(s) 66, 511, 742
BstZ17I GTATAC 1 cut(s) 406
BsuI GTATCC 1 cut(s) 764
BsuRI GGCC 6 cut(s) 91, 363, 571, 802, 1019, 1093
BtsCI GGATG 3 cut(s) 539, 623, 771
BtsIMutI CAGTG 3 cut(s) 153, 169, 358
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 7 cut(s) 33, 330, 372, 569, 706, 804, 946
Cfr10I RCCGGY 1 cut(s) 881
Cfr13I GGNCC 4 cut(s) 240, 435, 876, 1091
CseI GACGC 1 cut(s) 312
Csp6I GTAC 1 cut(s) 796
CspAI ACCGGT 1 cut(s) 881
CspCI CAANNNNNGTGG 2 cut(s) 825, 860
CviAII CATG 3 cut(s) 174, 365, 371
CviQI GTAC 1 cut(s) 796
DdeI CTNAG 2 cut(s) 976, 989
DpnI GATC 8 cut(s) 42, 68, 391, 425, 496, 513, 744, 910
DpnII GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
DriI GACNNNNNGTC 1 cut(s) 210
EaeI YGGCCR 3 cut(s) 361, 800, 1017
Eam1105I GACNNNNNGTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 1014
Eco147I AGGCCT 1 cut(s) 571
Eco24I GRGCYC 1 cut(s) 555
Eco31I GGTCTC 1 cut(s) 697
Eco47I GGWCC 3 cut(s) 240, 435, 876
Eco53kI GAGCTC 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 1093
EcoT14I CCWWGG 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 555
ErhI CCWWGG 1 cut(s) 1014
FaeI CATG 3 cut(s) 177, 368, 374
FaqI GGGAC 2 cut(s) 217, 235
FatI CATG 3 cut(s) 173, 364, 370
FauNDI CATATG 1 cut(s) 734
FblI GTMKAC 2 cut(s) 405, 972
Fnu4HI GCNGC 1 cut(s) 29
FokI GGATG 3 cut(s) 546, 630, 778
FriOI GRGCYC 1 cut(s) 555
Fsp4HI GCNGC 1 cut(s) 29
FspBI CTAG 4 cut(s) 573, 752, 788, 962
GluI GCNGC 1 cut(s) 29
GsaI CCCAGC 1 cut(s) 443
HaeIII GGCC 6 cut(s) 91, 363, 571, 802, 1019, 1093
HapII CCGG 5 cut(s) 238, 387, 548, 689, 882
HgaI GACGC 1 cut(s) 312
Hin1II CATG 3 cut(s) 177, 368, 374
HincII GTYRAC 1 cut(s) 299
HindII GTYRAC 1 cut(s) 299
HinfI GANTC 2 cut(s) 599, 974
HpaII CCGG 5 cut(s) 238, 387, 548, 689, 882
HphI GGTGA 6 cut(s) 35, 572, 574, 869, 877, 896
Hpy166II GTNNAC 5 cut(s) 299, 406, 582, 879, 973
Hpy188I TCNGA 6 cut(s) 216, 231, 263, 511, 956, 979
Hpy188III TCNNGA 6 cut(s) 70, 129, 492, 548, 639, 1045
Hpy8I GTNNAC 5 cut(s) 299, 406, 582, 879, 973
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 2 cut(s) 638, 733
HpyCH4III ACNGT 4 cut(s) 148, 226, 410, 659
HpyCH4IV ACGT 1 cut(s) 720
HpyCH4V TGCA 6 cut(s) 173, 338, 413, 649, 704, 923
HpyF10VI GCNNNNNNNGC 3 cut(s) 674, 808, 833
HpyF3I CTNAG 2 cut(s) 976, 989
HpySE526I ACGT 1 cut(s) 720
Hsp92II CATG 3 cut(s) 177, 368, 374
Kpn2I TCCGGA 1 cut(s) 547
Kzo9I GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
LmnI GCTCC 2 cut(s) 550, 674
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 2 cut(s) 325, 608
MaeI CTAG 4 cut(s) 573, 752, 788, 962
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 3 cut(s) 560, 721, 1034
MalI GATC 8 cut(s) 42, 68, 391, 425, 496, 513, 744, 910
MboI GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
MboII GAAGA 5 cut(s) 115, 117, 169, 938, 1110
MfeI CAATTG 1 cut(s) 1030
MflI RGATCY 3 cut(s) 66, 511, 742
MhlI GDGCHC 4 cut(s) 183, 555, 891, 1073
MlsI TGGCCA 2 cut(s) 363, 802
MluCI AATT 3 cut(s) 713, 757, 1030
MluNI TGGCCA 2 cut(s) 363, 802
MlyI GAGTC 1 cut(s) 968
MnlI CCTC 8 cut(s) 57, 212, 740, 853, 904, 958, 998, 1082
Mox20I TGGCCA 2 cut(s) 363, 802
MroI TCCGGA 1 cut(s) 547
MscI TGGCCA 2 cut(s) 363, 802
MseI TTAA 2 cut(s) 53, 315
MslI CAYNNNNRTG 3 cut(s) 198, 369, 902
Msp20I TGGCCA 2 cut(s) 363, 802
MspI CCGG 5 cut(s) 238, 387, 548, 689, 882
MspR9I CCNGG 2 cut(s) 689, 1095
MunI CAATTG 1 cut(s) 1030
MvaI CCWGG 1 cut(s) 1095
MwoI GCNNNNNNNGC 3 cut(s) 674, 808, 833
NciI CCSGG 1 cut(s) 689
NdeI CATATG 1 cut(s) 734
NdeII GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
NlaIII CATG 3 cut(s) 177, 368, 374
NlaIV GGNNCC 4 cut(s) 81, 182, 437, 744
NmeAIII GCCGAG 1 cut(s) 1045
NmuCI GTSAC 1 cut(s) 560
NspI RCATGY 1 cut(s) 374
PaeI GCATGC 1 cut(s) 374
PceI AGGCCT 1 cut(s) 571
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PfeI GAWTC 1 cut(s) 599
PflMI CCANNNNNTGG 1 cut(s) 76
PinAI ACCGGT 1 cut(s) 881
PkrI GCNGC 1 cut(s) 30
PleI GAGTC 1 cut(s) 968
PpsI GAGTC 1 cut(s) 968
PshBI ATTAAT 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 1093
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 1093
PspN4I GGNNCC 4 cut(s) 81, 182, 437, 744
PspPI GGNCC 4 cut(s) 240, 435, 876, 1091
PsuI RGATCY 3 cut(s) 66, 511, 742
RsaI GTAC 1 cut(s) 797
RsaNI GTAC 1 cut(s) 796
RseI CAYNNNNRTG 3 cut(s) 198, 369, 902
SacI GAGCTC 1 cut(s) 555
SaqAI TTAA 2 cut(s) 53, 315
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 8 cut(s) 40, 66, 389, 423, 494, 511, 742, 908
Sau96I GGNCC 4 cut(s) 240, 435, 876, 1091
SchI GAGTC 1 cut(s) 968
ScrFI CCNGG 2 cut(s) 689, 1095
SduI GDGCHC 4 cut(s) 183, 555, 891, 1073
SfaNI GCATC 2 cut(s) 325, 608
SgrAI CRCCGGYG 1 cut(s) 881
SinI GGWCC 3 cut(s) 240, 435, 876
SmiMI CAYNNNNRTG 3 cut(s) 198, 369, 902
SphI GCATGC 1 cut(s) 374
Sse9I AATT 3 cut(s) 713, 757, 1030
SseBI AGGCCT 1 cut(s) 571
SspI AATATT 1 cut(s) 313
SspMI CTAG 4 cut(s) 573, 752, 788, 962
SstI GAGCTC 1 cut(s) 555
StuI AGGCCT 1 cut(s) 571
StyD4I CCNGG 2 cut(s) 687, 1093
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 4 cut(s) 148, 226, 410, 659
TaiI ACGT 1 cut(s) 723
TaqI TCGA 4 cut(s) 130, 395, 447, 501
TasI AATT 3 cut(s) 713, 757, 1030
TfiI GAWTC 1 cut(s) 599
Tru1I TTAA 2 cut(s) 53, 315
Tru9I TTAA 2 cut(s) 53, 315
TscAI CASTG 3 cut(s) 153, 169, 358
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 6 cut(s) 5, 116, 125, 414, 470, 591
TspGWI ACGGA 1 cut(s) 789
TspRI CASTG 3 cut(s) 153, 169, 358
Van91I CCANNNNNTGG 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 240, 435, 876
VspI ATTAAT 1 cut(s) 315
XceI RCATGY 1 cut(s) 374
XmiI GTMKAC 2 cut(s) 405, 972
XspI CTAG 4 cut(s) 573, 752, 788, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.