Rmu_co8511853.1_g000001

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8511853.1
OG0013071
Physical Location & Seq
Forward (+)
1 .. 2046
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8511853.1_g000001.1.cds

Sequence Viewer

Length: 750 bp
catgcgattgcttacaccggatcatcgaatgaagtatacggtgcaaaagctacgatcaatgtttggaccccagcaatcgaacaaatgaacgagtttagtctttcgcaagtttgggttctgtcgggatcattcgatggttcagatctcaacagcatagaagctggatggcaggtcagtccggagctctatggtgacagcaggcctagattgttcacctattggacgactgattcatatcaggcaactggatgctacaaccttctgtgttctggatttgtgcaaacaaacagtagaatagccattggagcttccatctccccggtctcatcgtatgcaagcgaccaatttgacgttactatcctcatatggaaggatccaaagctaggaaattggtggatgggatatggagagaatagtctagtagggtactggccagcagagctattcacacacttggcagacagagcaacaatggtggaatggggtggtgaggtggtgaatacaaggtccaccggtgagcacacctccacccaaatgggttcgggtcattttgcagaagacgggtttgggaaagcaagctatttccgaaacctagaggttgtagactcagacaatagcctcagttctgtgcgagaagtctcaaccttggccgagaacaccaattgttacaacatcaagagtttctataacaacgattggggcacttacttctactatggagggccaggaagaaatccaaaatgcccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

249

Amino Acids

27.62

Weight (kDa)

4.63

Isoelectric Point (pI)

25.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
AccI GTMKAC 2 cut(s) 36, 603
AccIII TCCGGA 1 cut(s) 178
AclWI GGATC 4 cut(s) 28, 133, 368, 381
AcoI YGGCCR 2 cut(s) 431, 648
AfaI GTAC 1 cut(s) 428
AfiI CCNNNNNNNGG 1 cut(s) 383
AgeI ACCGGT 1 cut(s) 512
AjnI CCWGG 1 cut(s) 724
AjuI GAANNNNNNNTTGG 2 cut(s) 549, 581
AluBI AGCT 7 cut(s) 50, 161, 184, 308, 382, 442, 579
AluI AGCT 7 cut(s) 50, 161, 184, 308, 382, 442, 579
Alw21I GWGCWC 2 cut(s) 186, 522
Alw26I GTCTC 2 cut(s) 328, 643
AlwI GGATC 4 cut(s) 28, 133, 368, 381
Aor13HI TCCGGA 1 cut(s) 178
AoxI GGCC 4 cut(s) 200, 431, 648, 722
AsiGI ACCGGT 1 cut(s) 512
AspS9I GGNCC 3 cut(s) 66, 507, 722
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 5 cut(s) 203, 205, 500, 508, 527
AvaII GGWCC 2 cut(s) 66, 507
BaeGI GKGCMC 1 cut(s) 704
BalI TGGCCA 1 cut(s) 433
BamHI GGATCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 2 cut(s) 186, 522
BccI CCATC 4 cut(s) 128, 159, 320, 391
BciT130I CCWGG 1 cut(s) 726
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 328, 643
BfaI CTAG 4 cut(s) 204, 383, 419, 593
BfuAI ACCTGC 1 cut(s) 160
BglII AGATCT 1 cut(s) 142
Bme1390I CCNGG 2 cut(s) 320, 726
Bme18I GGWCC 2 cut(s) 66, 507
BmgT120I GGNCC 3 cut(s) 66, 507, 722
BmiI GGNNCC 2 cut(s) 68, 375
BmrFI CCNGG 2 cut(s) 320, 726
BmsI GCATC 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 564
BplI GAGNNNNNCTC 2 cut(s) 509, 541
BpuMI CCSGG 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 2 cut(s) 318, 645
BsaWI WCCGGW 3 cut(s) 17, 178, 512
Bsc4I CCNNNNNNNGG 1 cut(s) 383
Bse118I RCCGGY 1 cut(s) 512
Bse1I ACTGG 2 cut(s) 250, 434
Bse8I GATNNNNATC 1 cut(s) 234
BseAI TCCGGA 1 cut(s) 178
BseBI CCWGG 1 cut(s) 726
BseDI CCNNGG 2 cut(s) 318, 645
BseGI GGATG 3 cut(s) 170, 254, 402
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMII CTCAG 2 cut(s) 621, 634
BseNI ACTGG 2 cut(s) 250, 434
BseSI GKGCMC 1 cut(s) 704
BseYI CCCAGC 1 cut(s) 70
BshFI GGCC 4 cut(s) 202, 433, 650, 724
BshTI ACCGGT 1 cut(s) 512
BsiHKAI GWGCWC 2 cut(s) 186, 522
BsiSI CCGG 4 cut(s) 18, 179, 320, 513
BslI CCNNNNNNNGG 1 cut(s) 383
BsmAI GTCTC 2 cut(s) 328, 643
BsnI GGCC 4 cut(s) 202, 433, 650, 724
Bso31I GGTCTC 1 cut(s) 328
Bsp1286I GDGCHC 3 cut(s) 186, 522, 704
Bsp13I TCCGGA 1 cut(s) 178
Bsp143I GATC 5 cut(s) 20, 54, 125, 142, 373
BspANI GGCC 4 cut(s) 202, 433, 650, 724
BspCNI CTCAG 2 cut(s) 620, 633
BspEI TCCGGA 1 cut(s) 178
BspLI GGNNCC 2 cut(s) 68, 375
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 4 cut(s) 28, 133, 368, 381
BspTNI GGTCTC 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 512
BsrI ACTGG 2 cut(s) 250, 434
BssAI RCCGGY 1 cut(s) 512
BssECI CCNNGG 2 cut(s) 318, 645
BssMI GATC 5 cut(s) 20, 54, 125, 142, 373
BssNAI GTATAC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 645
Bst1107I GTATAC 1 cut(s) 37
Bst2UI CCWGG 1 cut(s) 726
Bst4CI ACNGT 2 cut(s) 41, 290
BstC8I GCNNGC 4 cut(s) 200, 337, 435, 577
BstDEI CTNAG 2 cut(s) 607, 620
BstF5I GGATG 3 cut(s) 170, 254, 402
BstKTI GATC 5 cut(s) 23, 57, 128, 145, 376
BstMAI GTCTC 2 cut(s) 328, 643
BstMBI GATC 5 cut(s) 20, 54, 125, 142, 373
BstMWI GCNNNNNNNGC 3 cut(s) 305, 439, 464
BstNI CCWGG 1 cut(s) 726
BstSCI CCNGG 2 cut(s) 318, 724
BstSLI GKGCMC 1 cut(s) 704
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 2 cut(s) 142, 373
BstXI CCANNNNNNTGG 1 cut(s) 535
BstYI RGATCY 2 cut(s) 142, 373
BstZ17I GTATAC 1 cut(s) 37
BsuRI GGCC 4 cut(s) 202, 433, 650, 724
BtsCI GGATG 3 cut(s) 170, 254, 402
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 4 cut(s) 200, 337, 435, 577
Cfr10I RCCGGY 1 cut(s) 512
Cfr13I GGNCC 3 cut(s) 66, 507, 722
Csp6I GTAC 1 cut(s) 427
CspAI ACCGGT 1 cut(s) 512
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 1 cut(s) 2
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 2 cut(s) 607, 620
DpnI GATC 5 cut(s) 22, 56, 127, 144, 375
DpnII GATC 5 cut(s) 20, 54, 125, 142, 373
EaeI YGGCCR 2 cut(s) 431, 648
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 328
Eco47I GGWCC 2 cut(s) 66, 507
Eco53kI GAGCTC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 645
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 645
FauNDI CATATG 1 cut(s) 365
FblI GTMKAC 2 cut(s) 36, 603
FokI GGATG 3 cut(s) 177, 261, 409
FriOI GRGCYC 1 cut(s) 186
FspBI CTAG 4 cut(s) 204, 383, 419, 593
GsaI CCCAGC 1 cut(s) 74
HaeIII GGCC 4 cut(s) 202, 433, 650, 724
HapII CCGG 4 cut(s) 18, 179, 320, 513
HinfI GANTC 2 cut(s) 230, 605
HpaII CCGG 4 cut(s) 18, 179, 320, 513
HphI GGTGA 5 cut(s) 203, 205, 500, 508, 527
Hpy166II GTNNAC 4 cut(s) 37, 213, 510, 604
Hpy188I TCNGA 3 cut(s) 142, 587, 610
Hpy188III TCNNGA 4 cut(s) 123, 179, 270, 676
Hpy8I GTNNAC 4 cut(s) 37, 213, 510, 604
HpyAV CCTTC 2 cut(s) 269, 364
HpyCH4III ACNGT 2 cut(s) 41, 290
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 4 cut(s) 44, 280, 335, 554
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 439, 464
HpyF3I CTNAG 2 cut(s) 607, 620
HpySE526I ACGT 1 cut(s) 351
Kpn2I TCCGGA 1 cut(s) 178
Kzo9I GATC 5 cut(s) 20, 54, 125, 142, 373
LmnI GCTCC 2 cut(s) 181, 305
LweI GCATC 1 cut(s) 239
MaeI CTAG 4 cut(s) 204, 383, 419, 593
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 3 cut(s) 191, 352, 665
MalI GATC 5 cut(s) 22, 56, 127, 144, 375
MboI GATC 5 cut(s) 20, 54, 125, 142, 373
MboII GAAGA 2 cut(s) 569, 741
MfeI CAATTG 1 cut(s) 661
MflI RGATCY 2 cut(s) 142, 373
MhlI GDGCHC 3 cut(s) 186, 522, 704
MlsI TGGCCA 1 cut(s) 433
MluCI AATT 3 cut(s) 344, 388, 661
MluNI TGGCCA 1 cut(s) 433
MlyI GAGTC 1 cut(s) 599
MnlI CCTC 6 cut(s) 371, 484, 535, 589, 629, 713
Mox20I TGGCCA 1 cut(s) 433
MroI TCCGGA 1 cut(s) 178
MscI TGGCCA 1 cut(s) 433
MslI CAYNNNNRTG 1 cut(s) 533
Msp20I TGGCCA 1 cut(s) 433
MspI CCGG 4 cut(s) 18, 179, 320, 513
MspR9I CCNGG 2 cut(s) 320, 726
MunI CAATTG 1 cut(s) 661
MvaI CCWGG 1 cut(s) 726
MwoI GCNNNNNNNGC 3 cut(s) 305, 439, 464
NciI CCSGG 1 cut(s) 320
NdeI CATATG 1 cut(s) 365
NdeII GATC 5 cut(s) 20, 54, 125, 142, 373
NlaIV GGNNCC 2 cut(s) 68, 375
NmeAIII GCCGAG 1 cut(s) 676
NmuCI GTSAC 1 cut(s) 191
PceI AGGCCT 1 cut(s) 202
PfeI GAWTC 1 cut(s) 230
PinAI ACCGGT 1 cut(s) 512
PleI GAGTC 1 cut(s) 599
PpsI GAGTC 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 724
PspFI CCCAGC 1 cut(s) 70
PspGI CCWGG 1 cut(s) 724
PspN4I GGNNCC 2 cut(s) 68, 375
PspPI GGNCC 3 cut(s) 66, 507, 722
PsuI RGATCY 2 cut(s) 142, 373
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
RseI CAYNNNNRTG 1 cut(s) 533
SacI GAGCTC 1 cut(s) 186
Sau3AI GATC 5 cut(s) 20, 54, 125, 142, 373
Sau96I GGNCC 3 cut(s) 66, 507, 722
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 2 cut(s) 320, 726
SduI GDGCHC 3 cut(s) 186, 522, 704
SfaNI GCATC 1 cut(s) 239
SgrAI CRCCGGYG 1 cut(s) 512
SinI GGWCC 2 cut(s) 66, 507
SmiMI CAYNNNNRTG 1 cut(s) 533
Sse9I AATT 3 cut(s) 344, 388, 661
SseBI AGGCCT 1 cut(s) 202
SspMI CTAG 4 cut(s) 204, 383, 419, 593
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 318, 724
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 2 cut(s) 41, 290
TaiI ACGT 1 cut(s) 354
TaqI TCGA 3 cut(s) 26, 78, 132
TasI AATT 3 cut(s) 344, 388, 661
TfiI GAWTC 1 cut(s) 230
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 3 cut(s) 45, 101, 222
VpaK11BI GGWCC 2 cut(s) 66, 507
XmiI GTMKAC 2 cut(s) 36, 603
XspI CTAG 4 cut(s) 204, 383, 419, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.