RLG00000025858

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013071
Physical Location & Seq
Reverse (-)
48702727 .. 48706171
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025858

Sequence Viewer

Length: 1335 bp
ATGAAAATGGATAGTCTTCTTCTTCTTCTACTTTTAGGCTTCCTTCTCACCCTCTTTTCCCAAAGATTTTCGCTTCCAGTAGTGGTGGCCTTGGACCTGAAAAACACCACAAGGTACAATAAGCAAGTCAGCACCTTGAGACTTGAGAGAATTGAGAAGCACTTGGACAAGATCAACAAGCCTCCTGTCATGACTATACAGAGCCCAGATGGAGATATGATTGATTGTGTTCATAAAAGAAAGCAGCCAGCTTTGGATCACCCTCTGTTAAAAAACCACAAGATCCAGAAAGTGGCACCAGAAAGGCCAAAAGTGATGAAGACGATGAAGCCATCATCATCTTCTCGAAGTGAGAATGATACAGTGAAAGCGAAGACCGGTGGGATTGCATGGGGGCACCAAATGTGGCATACAAATGGGACGAGGTGTCCGAGAGGGACTGTTCCGATACGACGGACCACAATGAGTGACGTTCTCCGAGCCAAGTCTTTGTTTGACTATGGCAAGAAAAGTCAACGACGCACCAATATTAATACGAATAGGCAAGCTGATGCACCCGATGTGGTCAGTGGGAATGGCCATGAGCATGCGATTGCTTACAGTGGATCATCAAATGAAGTATACGGTGCAAAAGCTACGATCAATGTTTGGACCCCAGCAATTGAACAAATGAACGAGTTTAGTCTTTCGCAAATTTGGGTTCTGTCGGGATCATTCGATGGTTCAGATCTCAACAGCATAGAAGCTGGATGGCAGGTCAGTCCGGAGCTCTATGGTGACAGCAGGCCTAGATTGTTCACCTATTGGACGACTGATTCATATCAGGCCACTGGGTGCTACAACCTTCTGTGTTCTGGATTTGTGCAAACAAACAGTAGAATAGCCATTGGAGCTTCCATCTCCCCGGTCTCATCGTATGCAAGCGACCAATTTGACGTTACTATCCTCATATGGAAGGATCCAAAGCTAGGAAATTGGTGGATGGGATATGGAGAGAATAGTCTAGTAGGGTACTGGCCAGCAGAGCTATTCACACACTTGGCAGACAGAGCAACAATGGTGGAATGGGGTGGTGAGGTGGTGAATACAAGGTCCACCGGTGAGCACACCTCCACCCAAATGGGTTCGGGTCATTTTGCAGAAGACGGGTTTGGGAAAGCAAGCTATTTCCGAAACCTAGAGGTTGTAGACTCAGACAATAGCCTCAGTTCTGTGCGAGAAGTCTCAACCTTGGCCGAGAACACCAATTGTTACAACATCAAGAGTTTCTATAACAACGATTGGGGCACTTACTTCTACTATGGAGGGCCAGGAAGAAATCCAAAATGCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

445

Amino Acids

49.7

Weight (kDa)

8.7

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 65 - 200 4e-42 Neprosin activation peptide
Neprosin PF03080 225 - 437 1.4e-88 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 745
AccB1I GGYRCC 2 cut(s) 295, 396
AccB7I CCANNNNNTGG 1 cut(s) 292
AccI GTMKAC 2 cut(s) 621, 1188
AccIII TCCGGA 1 cut(s) 763
AclWI GGATC 6 cut(s) 264, 277, 613, 718, 953, 966
AcoI YGGCCR 3 cut(s) 577, 1016, 1233
AcsI RAATTY 1 cut(s) 693
AdeI CACNNNGTG 1 cut(s) 834
AfaI GTAC 2 cut(s) 116, 1013
AfiI CCNNNNNNNGG 2 cut(s) 292, 968
AgeI ACCGGT 2 cut(s) 377, 1097
AgsI TTSAA 1 cut(s) 665
AhdI GACNNNNNGTC 1 cut(s) 426
AjnI CCWGG 1 cut(s) 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 1134, 1166
AluBI AGCT 9 cut(s) 251, 548, 635, 746, 769, 893, 967, 1027, 1164
AluI AGCT 9 cut(s) 251, 548, 635, 746, 769, 893, 967, 1027, 1164
Alw21I GWGCWC 2 cut(s) 771, 1107
Alw26I GTCTC 3 cut(s) 133, 913, 1228
AlwI GGATC 6 cut(s) 264, 277, 613, 718, 953, 966
Aor13HI TCCGGA 1 cut(s) 763
AoxI GGCC 8 cut(s) 87, 305, 577, 785, 825, 1016, 1233, 1307
ApeKI GCWGC 1 cut(s) 244
ApoI RAATTY 1 cut(s) 693
AseI ATTAAT 1 cut(s) 531
AsiGI ACCGGT 2 cut(s) 377, 1097
AspS9I GGNCC 5 cut(s) 94, 456, 651, 1092, 1307
AsuC2I CCSGG 1 cut(s) 905
AsuHPI GGTGA 7 cut(s) 40, 251, 788, 790, 1085, 1093, 1112
AvaII GGWCC 4 cut(s) 94, 456, 651, 1092
BaeGI GKGCMC 2 cut(s) 399, 1289
BalI TGGCCA 2 cut(s) 579, 1018
BamHI GGATCC 1 cut(s) 958
BanI GGYRCC 2 cut(s) 295, 396
BanII GRGCYC 2 cut(s) 206, 771
BbsI GAAGAC 4 cut(s) 8, 326, 380, 1149
Bbv12I GWGCWC 2 cut(s) 771, 1107
BbvI GCAGC 1 cut(s) 256
BccI CCATC 6 cut(s) 203, 340, 713, 744, 905, 976
BciT130I CCWGG 1 cut(s) 1311
BcnI CCSGG 1 cut(s) 905
BcoDI GTCTC 3 cut(s) 133, 913, 1228
BfaI CTAG 4 cut(s) 789, 968, 1004, 1178
BfuAI ACCTGC 1 cut(s) 745
BglII AGATCT 1 cut(s) 727
BisI GCNGC 1 cut(s) 245
BlsI GCNGC 1 cut(s) 246
Bme1390I CCNGG 2 cut(s) 905, 1311
Bme18I GGWCC 4 cut(s) 94, 456, 651, 1092
BmeRI GACNNNNNGTC 1 cut(s) 426
BmgT120I GGNCC 5 cut(s) 94, 456, 651, 1092, 1307
BmiI GGNNCC 4 cut(s) 297, 398, 653, 960
BmrFI CCNGG 2 cut(s) 905, 1311
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 840
BpiI GAAGAC 4 cut(s) 8, 326, 380, 1149
BplI GAGNNNNNCTC 2 cut(s) 1094, 1126
BpuEI CTTGAG 2 cut(s) 157, 164
BpuMI CCSGG 1 cut(s) 905
BsaBI GATNNNNATC 1 cut(s) 819
BsaI GGTCTC 1 cut(s) 913
BsaJI CCNNGG 3 cut(s) 90, 903, 1230
BsaWI WCCGGW 3 cut(s) 377, 763, 1097
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 968
Bse118I RCCGGY 2 cut(s) 377, 1097
Bse1I ACTGG 3 cut(s) 77, 835, 1019
Bse8I GATNNNNATC 1 cut(s) 819
BseAI TCCGGA 1 cut(s) 763
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 3 cut(s) 90, 903, 1230
BseGI GGATG 2 cut(s) 755, 987
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 2 cut(s) 292, 968
BseMII CTCAG 2 cut(s) 1206, 1219
BseNI ACTGG 3 cut(s) 77, 835, 1019
BseSI GKGCMC 2 cut(s) 399, 1289
BseXI GCAGC 1 cut(s) 256
BseYI CCCAGC 1 cut(s) 655
BshFI GGCC 8 cut(s) 89, 307, 579, 787, 827, 1018, 1235, 1309
BshNI GGYRCC 2 cut(s) 295, 396
BshTI ACCGGT 2 cut(s) 377, 1097
BsiHKAI GWGCWC 2 cut(s) 771, 1107
BsiSI CCGG 4 cut(s) 378, 764, 905, 1098
BslFI GGGAC 2 cut(s) 433, 451
BslI CCNNNNNNNGG 2 cut(s) 292, 968
BsmAI GTCTC 3 cut(s) 133, 913, 1228
BsmFI GGGAC 2 cut(s) 433, 451
BsnI GGCC 8 cut(s) 89, 307, 579, 787, 827, 1018, 1235, 1309
Bso31I GGTCTC 1 cut(s) 913
Bsp1286I GDGCHC 5 cut(s) 206, 399, 771, 1107, 1289
Bsp13I TCCGGA 1 cut(s) 763
Bsp143I GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
BspANI GGCC 8 cut(s) 89, 307, 579, 787, 827, 1018, 1235, 1309
BspCNI CTCAG 2 cut(s) 1205, 1218
BspEI TCCGGA 1 cut(s) 763
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 4 cut(s) 297, 398, 653, 960
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 6 cut(s) 264, 277, 613, 718, 953, 966
BspT107I GGYRCC 2 cut(s) 295, 396
BspTNI GGTCTC 1 cut(s) 913
BsrFI RCCGGY 2 cut(s) 377, 1097
BsrI ACTGG 3 cut(s) 77, 835, 1019
BssAI RCCGGY 2 cut(s) 377, 1097
BssECI CCNNGG 3 cut(s) 90, 903, 1230
BssMI GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
BssNAI GTATAC 1 cut(s) 622
BssT1I CCWWGG 2 cut(s) 90, 1230
Bst1107I GTATAC 1 cut(s) 622
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 5 cut(s) 364, 442, 602, 626, 875
BstC8I GCNNGC 7 cut(s) 249, 546, 588, 785, 922, 1020, 1162
BstDEI CTNAG 2 cut(s) 1192, 1205
BstF5I GGATG 2 cut(s) 755, 987
BstKTI GATC 8 cut(s) 174, 259, 285, 608, 642, 713, 730, 961
BstMAI GTCTC 3 cut(s) 133, 913, 1228
BstMBI GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
BstMWI GCNNNNNNNGC 3 cut(s) 890, 1024, 1049
BstNI CCWGG 1 cut(s) 1311
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 903, 1309
BstSLI GKGCMC 2 cut(s) 399, 1289
BstV1I GCAGC 1 cut(s) 256
BstV2I GAAGAC 4 cut(s) 8, 326, 380, 1149
BstX2I RGATCY 3 cut(s) 282, 727, 958
BstXI CCANNNNNNTGG 1 cut(s) 1120
BstYI RGATCY 3 cut(s) 282, 727, 958
BstZ17I GTATAC 1 cut(s) 622
BsuRI GGCC 8 cut(s) 89, 307, 579, 787, 827, 1018, 1235, 1309
BtsCI GGATG 2 cut(s) 755, 987
BtsIMutI CAGTG 4 cut(s) 369, 574, 607, 828
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 7 cut(s) 249, 546, 588, 785, 922, 1020, 1162
CciI TCATGA 1 cut(s) 189
Cfr10I RCCGGY 2 cut(s) 377, 1097
Cfr13I GGNCC 5 cut(s) 94, 456, 651, 1092, 1307
CseI GACGC 1 cut(s) 528
Csp6I GTAC 2 cut(s) 115, 1012
CspAI ACCGGT 2 cut(s) 377, 1097
CspCI CAANNNNNGTGG 2 cut(s) 1041, 1076
CviAII CATG 5 cut(s) 190, 390, 581, 587, 1332
CviQI GTAC 2 cut(s) 115, 1012
DdeI CTNAG 2 cut(s) 1192, 1205
DpnI GATC 8 cut(s) 173, 258, 284, 607, 641, 712, 729, 960
DpnII GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
DraIII CACNNNGTG 1 cut(s) 834
DriI GACNNNNNGTC 1 cut(s) 426
EaeI YGGCCR 3 cut(s) 577, 1016, 1233
Eam1105I GACNNNNNGTC 1 cut(s) 426
Ecl136II GAGCTC 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 90, 1230
Eco147I AGGCCT 1 cut(s) 787
Eco24I GRGCYC 2 cut(s) 206, 771
Eco31I GGTCTC 1 cut(s) 913
Eco47I GGWCC 4 cut(s) 94, 456, 651, 1092
Eco53kI GAGCTC 1 cut(s) 769
EcoICRI GAGCTC 1 cut(s) 769
EcoRII CCWGG 1 cut(s) 1309
EcoT14I CCWWGG 2 cut(s) 90, 1230
EcoT38I GRGCYC 2 cut(s) 206, 771
ErhI CCWWGG 2 cut(s) 90, 1230
FaeI CATG 5 cut(s) 193, 393, 584, 590, 1335
FaqI GGGAC 2 cut(s) 433, 451
FatI CATG 5 cut(s) 189, 389, 580, 586, 1331
FauNDI CATATG 1 cut(s) 950
FblI GTMKAC 2 cut(s) 621, 1188
Fnu4HI GCNGC 1 cut(s) 245
FokI GGATG 2 cut(s) 762, 994
FriOI GRGCYC 2 cut(s) 206, 771
Fsp4HI GCNGC 1 cut(s) 245
FspBI CTAG 4 cut(s) 789, 968, 1004, 1178
GluI GCNGC 1 cut(s) 245
GsaI CCCAGC 1 cut(s) 659
HaeIII GGCC 8 cut(s) 89, 307, 579, 787, 827, 1018, 1235, 1309
HapII CCGG 4 cut(s) 378, 764, 905, 1098
HgaI GACGC 1 cut(s) 528
Hin1II CATG 5 cut(s) 193, 393, 584, 590, 1335
HincII GTYRAC 1 cut(s) 515
HindII GTYRAC 1 cut(s) 515
HinfI GANTC 2 cut(s) 815, 1190
HpaII CCGG 4 cut(s) 378, 764, 905, 1098
HphI GGTGA 7 cut(s) 40, 251, 788, 790, 1085, 1093, 1112
Hpy166II GTNNAC 5 cut(s) 515, 622, 798, 1095, 1189
Hpy188I TCNGA 6 cut(s) 432, 447, 479, 727, 1172, 1195
Hpy188III TCNNGA 7 cut(s) 190, 286, 345, 708, 764, 855, 1261
Hpy8I GTNNAC 5 cut(s) 515, 622, 798, 1095, 1189
Hpy99I CGWCG 2 cut(s) 456, 522
HpyAV CCTTC 3 cut(s) 53, 854, 949
HpyCH4III ACNGT 5 cut(s) 364, 442, 602, 626, 875
HpyCH4IV ACGT 2 cut(s) 471, 936
HpyCH4V TGCA 6 cut(s) 389, 554, 629, 865, 920, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 890, 1024, 1049
HpyF3I CTNAG 2 cut(s) 1192, 1205
HpySE526I ACGT 2 cut(s) 471, 936
Hsp92II CATG 5 cut(s) 193, 393, 584, 590, 1335
Kpn2I TCCGGA 1 cut(s) 763
Kzo9I GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
LmnI GCTCC 2 cut(s) 766, 890
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 541
MaeI CTAG 4 cut(s) 789, 968, 1004, 1178
MaeII ACGT 2 cut(s) 471, 936
MaeIII GTNAC 4 cut(s) 467, 776, 937, 1250
MalI GATC 8 cut(s) 173, 258, 284, 607, 641, 712, 729, 960
MboI GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
MboII GAAGA 9 cut(s) 8, 11, 14, 17, 331, 333, 385, 1154, 1326
MfeI CAATTG 2 cut(s) 660, 1246
MflI RGATCY 3 cut(s) 282, 727, 958
MhlI GDGCHC 5 cut(s) 206, 399, 771, 1107, 1289
MlsI TGGCCA 2 cut(s) 579, 1018
MluCI AATT 6 cut(s) 150, 660, 693, 929, 973, 1246
MluNI TGGCCA 2 cut(s) 579, 1018
MlyI GAGTC 1 cut(s) 1184
Mox20I TGGCCA 2 cut(s) 579, 1018
MroI TCCGGA 1 cut(s) 763
MscI TGGCCA 2 cut(s) 579, 1018
MseI TTAA 2 cut(s) 269, 531
MslI CAYNNNNRTG 3 cut(s) 414, 585, 1118
Msp20I TGGCCA 2 cut(s) 579, 1018
MspI CCGG 4 cut(s) 378, 764, 905, 1098
MspR9I CCNGG 2 cut(s) 905, 1311
MunI CAATTG 2 cut(s) 660, 1246
MvaI CCWGG 1 cut(s) 1311
MwoI GCNNNNNNNGC 3 cut(s) 890, 1024, 1049
NciI CCSGG 1 cut(s) 905
NdeI CATATG 1 cut(s) 950
NdeII GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
NlaIII CATG 5 cut(s) 193, 393, 584, 590, 1335
NlaIV GGNNCC 4 cut(s) 297, 398, 653, 960
NmeAIII GCCGAG 1 cut(s) 1261
NmuCI GTSAC 2 cut(s) 467, 776
NspI RCATGY 1 cut(s) 590
PaeI GCATGC 1 cut(s) 590
PagI TCATGA 1 cut(s) 189
PceI AGGCCT 1 cut(s) 787
PcsI WCGNNNNNNNCGW 1 cut(s) 428
PfeI GAWTC 1 cut(s) 815
PflMI CCANNNNNTGG 1 cut(s) 292
PinAI ACCGGT 2 cut(s) 377, 1097
PkrI GCNGC 1 cut(s) 246
PleI GAGTC 1 cut(s) 1184
PpsI GAGTC 1 cut(s) 1184
PshBI ATTAAT 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 771
Psp6I CCWGG 1 cut(s) 1309
PspFI CCCAGC 1 cut(s) 655
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 4 cut(s) 297, 398, 653, 960
PspPI GGNCC 5 cut(s) 94, 456, 651, 1092, 1307
PsuI RGATCY 3 cut(s) 282, 727, 958
RsaI GTAC 2 cut(s) 116, 1013
RsaNI GTAC 2 cut(s) 115, 1012
RseI CAYNNNNRTG 3 cut(s) 414, 585, 1118
SacI GAGCTC 1 cut(s) 771
SaqAI TTAA 2 cut(s) 269, 531
SatI GCNGC 1 cut(s) 245
Sau3AI GATC 8 cut(s) 171, 256, 282, 605, 639, 710, 727, 958
Sau96I GGNCC 5 cut(s) 94, 456, 651, 1092, 1307
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 2 cut(s) 905, 1311
SduI GDGCHC 5 cut(s) 206, 399, 771, 1107, 1289
SfaNI GCATC 1 cut(s) 541
SgrAI CRCCGGYG 1 cut(s) 1097
SinI GGWCC 4 cut(s) 94, 456, 651, 1092
SmiMI CAYNNNNRTG 3 cut(s) 414, 585, 1118
SmlI CTYRAG 2 cut(s) 136, 143
SmoI CTYRAG 2 cut(s) 136, 143
SphI GCATGC 1 cut(s) 590
Sse9I AATT 6 cut(s) 150, 660, 693, 929, 973, 1246
SseBI AGGCCT 1 cut(s) 787
SspI AATATT 1 cut(s) 529
SspMI CTAG 4 cut(s) 789, 968, 1004, 1178
SstI GAGCTC 1 cut(s) 771
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 2 cut(s) 903, 1309
StyI CCWWGG 2 cut(s) 90, 1230
TaaI ACNGT 5 cut(s) 364, 442, 602, 626, 875
TaiI ACGT 2 cut(s) 474, 939
TaqI TCGA 2 cut(s) 346, 717
TasI AATT 6 cut(s) 150, 660, 693, 929, 973, 1246
TfiI GAWTC 1 cut(s) 815
Tru1I TTAA 2 cut(s) 269, 531
Tru9I TTAA 2 cut(s) 269, 531
TscAI CASTG 4 cut(s) 369, 574, 607, 835
TseFI GTSAC 2 cut(s) 467, 776
TseI GCWGC 1 cut(s) 244
Tsp45I GTSAC 2 cut(s) 467, 776
TspDTI ATGAA 7 cut(s) 17, 221, 332, 341, 630, 686, 807
TspGWI ACGGA 1 cut(s) 469
TspRI CASTG 4 cut(s) 369, 574, 607, 835
Van91I CCANNNNNTGG 1 cut(s) 292
VpaK11BI GGWCC 4 cut(s) 94, 456, 651, 1092
VspI ATTAAT 1 cut(s) 531
XapI RAATTY 1 cut(s) 693
XceI RCATGY 1 cut(s) 590
XmiI GTMKAC 2 cut(s) 621, 1188
XspI CTAG 4 cut(s) 789, 968, 1004, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.