Rroxscaffold_6G00427160

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013071
Physical Location & Seq
Forward (+)
47796156 .. 47800606
4451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427160.1

Sequence Viewer

Length: 1278 bp
ATGAAAATGGATAGTCTTCTTCTTCTACTTTTAGGCTTCCTTGTCACCCTCTTTTCCCAAAGATTTTCGCTTCCAGTAGTGGTGGCCTTGGACCTGAAAAACACCACAAGGTACAATAAGCAAGTCAGCACCTTGAGACTTGAGAGAATTGACAAGCACTTGGACAAGATCAACAAGCCTCCTGTCATGACTATACAGAGCCCAGATGGAGATATGATTGATTGTGTTCATAAAAGAAAGCAGCCAGCTCTAGATCACCCTCTGTTAAAAAACCACAAGATCCAGAAAGTGGCACCAGAAAGGCCAAAAGTGATGAAGACGATGAAGCCATCATCATCTTCTCGAAGTGAGAATGATACAGTGAAAGCGAAGACCGGTGGGATCGCATGGGGGCACCAAATGTGGCATACAAATGGGACGAGGTGTCCGAGAGGGACTGTTCCAATACGGCGGACCACAATGAGTGATGTTCTCCGAGCCAAGTCCTTGTTTGACTATGGCAAGAAAAGTCAACGGCGCACCAGTATTAATACGAATAGGCAAGCTGATGCACCCGATGTGGTCAGTGGGAATGGCCATGAGCATGCGATTGCTTACACCGGATCATCGAATGAAGTATACGGTGCAAAAGCTACGATCAATGTTTGGACCCCAGCAATCGAACAAATGAACGAGTTTAGTCTTTCGCAAATTTGGGTTCTGTCGGGATCATTCGATGGTTCAGATCTCAACAGCATAGAAGCTGGATGGCAGACTGATTCATATCAGGCAACTGGGTGCTACAACCTTCTGTGTTCTGGATTTGTGCAAACAAACAGTAGAATAGCCATTGGAGCTTCCATCTCCCCGGTCTCATCGTATGCAAGCGACCAATTTGACGTTACTATCCTCATATGGAAGGATCCAAAGCTAGGAAATTGGTGGATGGGATATGGAGAGAATAGTCTAGTAGGGTACTGGCCAGCAGAGCTATTCACACACTTGGCAGACAGAGCAACAATGGTGGAATGGGGTGGTGAGGTGGTGAATACAAGGTCCACCGGTGAGCACACCTCCACCCAAATGGGTTCGGGTCATTTTGCAGAAGACGGGTTTGGGAAAGCAAGCTATTTCCGAAACCTAGAGGTTGTGGACTCAGACAATAGCCTCAGTTCTGTGCGAGAAGTCTCAACCTTGGCCGAGAACACCAATTGTTACAACATCAAGAGTTTCTATAACAACGATTGGGGCACTTACTTCTACTATGGAGGGCCAGGAAGAAATCCAAAATGCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

425

Amino Acids

47.38

Weight (kDa)

8.72

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 64 - 199 3.9e-42 Neprosin activation peptide
Neprosin PF03080 250 - 418 5.8e-67 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 292, 393
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 618
AciI CCGC 1 cut(s) 451
AclWI GGATC 6 cut(s) 274, 389, 610, 715, 896, 909
AcoI YGGCCR 3 cut(s) 574, 959, 1176
AcsI RAATTY 1 cut(s) 690
AfaI GTAC 2 cut(s) 113, 956
AfiI CCNNNNNNNGG 2 cut(s) 289, 911
AgeI ACCGGT 2 cut(s) 374, 1040
AhdI GACNNNNNGTC 1 cut(s) 423
AjnI CCWGG 1 cut(s) 1252
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AluBI AGCT 8 cut(s) 248, 545, 632, 743, 836, 910, 970, 1107
AluI AGCT 8 cut(s) 248, 545, 632, 743, 836, 910, 970, 1107
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 3 cut(s) 130, 856, 1171
AlwI GGATC 6 cut(s) 274, 389, 610, 715, 896, 909
AoxI GGCC 6 cut(s) 84, 302, 574, 959, 1176, 1250
ApeKI GCWGC 1 cut(s) 241
ApoI RAATTY 1 cut(s) 690
AseI ATTAAT 1 cut(s) 528
AsiGI ACCGGT 2 cut(s) 374, 1040
AspLEI GCGC 1 cut(s) 519
AspS9I GGNCC 5 cut(s) 91, 453, 648, 1035, 1250
AsuC2I CCSGG 1 cut(s) 848
AsuHPI GGTGA 5 cut(s) 37, 248, 1028, 1036, 1055
AvaII GGWCC 4 cut(s) 91, 453, 648, 1035
BaeGI GKGCMC 2 cut(s) 396, 1232
BalI TGGCCA 2 cut(s) 576, 961
BamHI GGATCC 1 cut(s) 901
BanI GGYRCC 2 cut(s) 292, 393
BanII GRGCYC 1 cut(s) 203
BbsI GAAGAC 4 cut(s) 8, 323, 377, 1092
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 1 cut(s) 253
BccI CCATC 6 cut(s) 200, 337, 710, 741, 848, 919
BceAI ACGGC 2 cut(s) 464, 530
BciT130I CCWGG 1 cut(s) 1254
BcnI CCSGG 1 cut(s) 848
BcoDI GTCTC 3 cut(s) 130, 856, 1171
BfaI CTAG 4 cut(s) 251, 911, 947, 1121
BglII AGATCT 1 cut(s) 724
BisI GCNGC 1 cut(s) 242
BlsI GCNGC 1 cut(s) 243
Bme1390I CCNGG 2 cut(s) 848, 1254
Bme18I GGWCC 4 cut(s) 91, 453, 648, 1035
BmeRI GACNNNNNGTC 1 cut(s) 423
BmgT120I GGNCC 5 cut(s) 91, 453, 648, 1035, 1250
BmiI GGNNCC 4 cut(s) 294, 395, 650, 903
BmrFI CCNGG 2 cut(s) 848, 1254
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 1 cut(s) 538
BmuI ACTGGG 1 cut(s) 783
BpiI GAAGAC 4 cut(s) 8, 323, 377, 1092
BplI GAGNNNNNCTC 2 cut(s) 1037, 1069
BpuEI CTTGAG 2 cut(s) 154, 161
BpuMI CCSGG 1 cut(s) 848
BsaBI GATNNNNATC 1 cut(s) 762
BsaI GGTCTC 1 cut(s) 856
BsaJI CCNNGG 3 cut(s) 87, 846, 1173
BsaWI WCCGGW 3 cut(s) 374, 599, 1040
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 911
Bse118I RCCGGY 2 cut(s) 374, 1040
Bse1I ACTGG 4 cut(s) 74, 522, 778, 962
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 1 cut(s) 1254
BseDI CCNNGG 3 cut(s) 87, 846, 1173
BseGI GGATG 2 cut(s) 752, 930
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 2 cut(s) 289, 911
BseMII CTCAG 2 cut(s) 1149, 1162
BseNI ACTGG 4 cut(s) 74, 522, 778, 962
BseSI GKGCMC 2 cut(s) 396, 1232
BseXI GCAGC 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 652
BshFI GGCC 6 cut(s) 86, 304, 576, 961, 1178, 1252
BshNI GGYRCC 2 cut(s) 292, 393
BshTI ACCGGT 2 cut(s) 374, 1040
BsiHKAI GWGCWC 1 cut(s) 1050
BsiSI CCGG 4 cut(s) 375, 600, 848, 1041
BslFI GGGAC 2 cut(s) 430, 448
BslI CCNNNNNNNGG 2 cut(s) 289, 911
BsmAI GTCTC 3 cut(s) 130, 856, 1171
BsmFI GGGAC 2 cut(s) 430, 448
BsnI GGCC 6 cut(s) 86, 304, 576, 961, 1178, 1252
Bso31I GGTCTC 1 cut(s) 856
Bsp1286I GDGCHC 4 cut(s) 203, 396, 1050, 1232
Bsp143I GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
BspACI CCGC 1 cut(s) 451
BspANI GGCC 6 cut(s) 86, 304, 576, 961, 1178, 1252
BspCNI CTCAG 2 cut(s) 1148, 1161
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 4 cut(s) 294, 395, 650, 903
BspPI GGATC 6 cut(s) 274, 389, 610, 715, 896, 909
BspT107I GGYRCC 2 cut(s) 292, 393
BspTNI GGTCTC 1 cut(s) 856
BsrFI RCCGGY 2 cut(s) 374, 1040
BsrI ACTGG 4 cut(s) 74, 522, 778, 962
BssAI RCCGGY 2 cut(s) 374, 1040
BssECI CCNNGG 3 cut(s) 87, 846, 1173
BssMI GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
BssNAI GTATAC 1 cut(s) 619
BssT1I CCWWGG 2 cut(s) 87, 1173
Bst1107I GTATAC 1 cut(s) 619
Bst2UI CCWGG 1 cut(s) 1254
Bst4CI ACNGT 4 cut(s) 361, 439, 623, 818
BstC8I GCNNGC 6 cut(s) 246, 543, 585, 865, 963, 1105
BstDEI CTNAG 2 cut(s) 1135, 1148
BstF5I GGATG 2 cut(s) 752, 930
BstHHI GCGC 1 cut(s) 519
BstKTI GATC 9 cut(s) 171, 256, 282, 384, 605, 639, 710, 727, 904
BstMAI GTCTC 3 cut(s) 130, 856, 1171
BstMBI GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
BstMWI GCNNNNNNNGC 3 cut(s) 833, 967, 992
BstNI CCWGG 1 cut(s) 1254
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 846, 1252
BstSLI GKGCMC 2 cut(s) 396, 1232
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 4 cut(s) 8, 323, 377, 1092
BstX2I RGATCY 3 cut(s) 279, 724, 901
BstXI CCANNNNNNTGG 1 cut(s) 1063
BstYI RGATCY 3 cut(s) 279, 724, 901
BstZ17I GTATAC 1 cut(s) 619
BsuRI GGCC 6 cut(s) 86, 304, 576, 961, 1178, 1252
BtsCI GGATG 2 cut(s) 752, 930
BtsIMutI CAGTG 2 cut(s) 366, 571
Cac8I GCNNGC 6 cut(s) 246, 543, 585, 865, 963, 1105
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 519
Cfr10I RCCGGY 2 cut(s) 374, 1040
Cfr13I GGNCC 5 cut(s) 91, 453, 648, 1035, 1250
Csp6I GTAC 2 cut(s) 112, 955
CspAI ACCGGT 2 cut(s) 374, 1040
CspCI CAANNNNNGTGG 2 cut(s) 984, 1019
CviAII CATG 5 cut(s) 187, 387, 578, 584, 1275
CviQI GTAC 2 cut(s) 112, 955
DdeI CTNAG 2 cut(s) 1135, 1148
DpnI GATC 9 cut(s) 170, 255, 281, 383, 604, 638, 709, 726, 903
DpnII GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
DriI GACNNNNNGTC 1 cut(s) 423
EaeI YGGCCR 3 cut(s) 574, 959, 1176
Eam1105I GACNNNNNGTC 1 cut(s) 423
EciI GGCGGA 1 cut(s) 466
Eco130I CCWWGG 2 cut(s) 87, 1173
Eco24I GRGCYC 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 856
Eco47I GGWCC 4 cut(s) 91, 453, 648, 1035
EcoRII CCWGG 1 cut(s) 1252
EcoT14I CCWWGG 2 cut(s) 87, 1173
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 2 cut(s) 87, 1173
FaeI CATG 5 cut(s) 190, 390, 581, 587, 1278
FaqI GGGAC 2 cut(s) 430, 448
FatI CATG 5 cut(s) 186, 386, 577, 583, 1274
FauNDI CATATG 1 cut(s) 893
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 1 cut(s) 242
FokI GGATG 2 cut(s) 759, 937
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 242
FspBI CTAG 4 cut(s) 251, 911, 947, 1121
GlaI GCGC 1 cut(s) 518
GluI GCNGC 1 cut(s) 242
GsaI CCCAGC 1 cut(s) 656
HaeIII GGCC 6 cut(s) 86, 304, 576, 961, 1178, 1252
HapII CCGG 4 cut(s) 375, 600, 848, 1041
HhaI GCGC 1 cut(s) 519
Hin1II CATG 5 cut(s) 190, 390, 581, 587, 1278
Hin6I GCGC 1 cut(s) 517
HinP1I GCGC 1 cut(s) 517
HincII GTYRAC 1 cut(s) 512
HindII GTYRAC 1 cut(s) 512
HinfI GANTC 2 cut(s) 758, 1133
HpaII CCGG 4 cut(s) 375, 600, 848, 1041
HphI GGTGA 5 cut(s) 37, 248, 1028, 1036, 1055
Hpy166II GTNNAC 4 cut(s) 512, 619, 1038, 1132
Hpy188I TCNGA 5 cut(s) 429, 476, 724, 1115, 1138
Hpy188III TCNNGA 7 cut(s) 187, 251, 283, 342, 705, 798, 1204
Hpy8I GTNNAC 4 cut(s) 512, 619, 1038, 1132
HpyAV CCTTC 2 cut(s) 797, 892
HpyCH4III ACNGT 4 cut(s) 361, 439, 623, 818
HpyCH4IV ACGT 1 cut(s) 879
HpyCH4V TGCA 5 cut(s) 551, 626, 808, 863, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 833, 967, 992
HpyF3I CTNAG 2 cut(s) 1135, 1148
HpySE526I ACGT 1 cut(s) 879
Hsp92II CATG 5 cut(s) 190, 390, 581, 587, 1278
HspAI GCGC 1 cut(s) 517
Kzo9I GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
LmnI GCTCC 1 cut(s) 833
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 1 cut(s) 538
MaeI CTAG 4 cut(s) 251, 911, 947, 1121
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 3 cut(s) 43, 880, 1193
MalI GATC 9 cut(s) 170, 255, 281, 383, 604, 638, 709, 726, 903
MboI GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
MboII GAAGA 8 cut(s) 8, 11, 14, 328, 330, 382, 1097, 1269
MfeI CAATTG 1 cut(s) 1189
MflI RGATCY 3 cut(s) 279, 724, 901
MhlI GDGCHC 4 cut(s) 203, 396, 1050, 1232
MlsI TGGCCA 2 cut(s) 576, 961
MluCI AATT 5 cut(s) 147, 690, 872, 916, 1189
MluNI TGGCCA 2 cut(s) 576, 961
MlyI GAGTC 1 cut(s) 1127
Mox20I TGGCCA 2 cut(s) 576, 961
MscI TGGCCA 2 cut(s) 576, 961
MseI TTAA 2 cut(s) 266, 528
MslI CAYNNNNRTG 3 cut(s) 411, 582, 1061
Msp20I TGGCCA 2 cut(s) 576, 961
MspI CCGG 4 cut(s) 375, 600, 848, 1041
MspR9I CCNGG 2 cut(s) 848, 1254
MunI CAATTG 1 cut(s) 1189
MvaI CCWGG 1 cut(s) 1254
MwoI GCNNNNNNNGC 3 cut(s) 833, 967, 992
NciI CCSGG 1 cut(s) 848
NdeI CATATG 1 cut(s) 893
NdeII GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
NlaIII CATG 5 cut(s) 190, 390, 581, 587, 1278
NlaIV GGNNCC 4 cut(s) 294, 395, 650, 903
NmeAIII GCCGAG 1 cut(s) 1204
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 587
PaeI GCATGC 1 cut(s) 587
PagI TCATGA 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PfeI GAWTC 1 cut(s) 758
PflMI CCANNNNNTGG 1 cut(s) 289
PinAI ACCGGT 2 cut(s) 374, 1040
PkrI GCNGC 1 cut(s) 243
PleI GAGTC 1 cut(s) 1127
PpsI GAGTC 1 cut(s) 1127
PshBI ATTAAT 1 cut(s) 528
Psp6I CCWGG 1 cut(s) 1252
PspFI CCCAGC 1 cut(s) 652
PspGI CCWGG 1 cut(s) 1252
PspN4I GGNNCC 4 cut(s) 294, 395, 650, 903
PspPI GGNCC 5 cut(s) 91, 453, 648, 1035, 1250
PsuI RGATCY 3 cut(s) 279, 724, 901
RsaI GTAC 2 cut(s) 113, 956
RsaNI GTAC 2 cut(s) 112, 955
RseI CAYNNNNRTG 3 cut(s) 411, 582, 1061
SaqAI TTAA 2 cut(s) 266, 528
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 9 cut(s) 168, 253, 279, 381, 602, 636, 707, 724, 901
Sau96I GGNCC 5 cut(s) 91, 453, 648, 1035, 1250
SchI GAGTC 1 cut(s) 1127
ScrFI CCNGG 2 cut(s) 848, 1254
SduI GDGCHC 4 cut(s) 203, 396, 1050, 1232
SfaNI GCATC 1 cut(s) 538
SgrAI CRCCGGYG 1 cut(s) 1040
SinI GGWCC 4 cut(s) 91, 453, 648, 1035
SmiMI CAYNNNNRTG 3 cut(s) 411, 582, 1061
SmlI CTYRAG 2 cut(s) 133, 140
SmoI CTYRAG 2 cut(s) 133, 140
SphI GCATGC 1 cut(s) 587
Sse9I AATT 5 cut(s) 147, 690, 872, 916, 1189
SsiI CCGC 1 cut(s) 451
SspMI CTAG 4 cut(s) 251, 911, 947, 1121
StyD4I CCNGG 2 cut(s) 846, 1252
StyI CCWWGG 2 cut(s) 87, 1173
TaaI ACNGT 4 cut(s) 361, 439, 623, 818
TaiI ACGT 1 cut(s) 882
TaqI TCGA 4 cut(s) 343, 608, 660, 714
TasI AATT 5 cut(s) 147, 690, 872, 916, 1189
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 2 cut(s) 266, 528
Tru9I TTAA 2 cut(s) 266, 528
TscAI CASTG 2 cut(s) 366, 571
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 7 cut(s) 17, 218, 329, 338, 627, 683, 750
TspRI CASTG 2 cut(s) 366, 571
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 4 cut(s) 91, 453, 648, 1035
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 1 cut(s) 690
XbaI TCTAGA 1 cut(s) 250
XceI RCATGY 1 cut(s) 587
XmiI GTMKAC 1 cut(s) 618
XspI CTAG 4 cut(s) 251, 911, 947, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.