MD04G1233600.v1.1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013071
Physical Location & Seq
Reverse (-)
31274656 .. 31280057
5402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1233600.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGGGGACTACTACTACTCTTGGTGGAAGGAACAAGAGAGAAAAGCACTTGTCACCGGTTCTTCTTCTGAAGCACTTTTTTCTCATCAGCATATTTTTCCAAAATTTTTCACTTTCTGTGGAAGCTGCTTATTCTGGAAAGCTGAAATACACAAGGCATAATAAGCAAGTTAGCAGCTTGAGACTGGAAAGGATTGAGAAGCACTTGAACAAGATCAACAAGCCAGCTGTTATGACCATTCAAAGTCCAGATGGAGATATCATAGATTGCGTTCATAAAAGAAAACAGCCAGCTTTGGATCACCCTCTTTTAAAGAATCACAAGATCCAGAGGAAGGCACCAGAAAGGCCAAAAGCAGTAGTAACAAAGAAGATGAAGCAAAATAATGATGACGAATTAGATGGGATCAAGGATAATAATAATGTGATTAATGCCACTGCTAATCAAGGAGGAGGAGGAGTGATTAAGGGTGCATGGGTACTGCAGCAAATGTGGCATAGAAATGGGACAAGATGTCCGAGAGGAACGGTTCCTATACGGCGGAGCACAATGGATGATGTGCTGAGGGCTAAGTCTTTGTTTGACTTTGGAAAGAAACAGCATCGTACCAGTTTTAATATTAATAATAAGAATATTACTGCTGATCTTGCTTCCAGACGCGTCGATGCCCCCGATGTGGTCAGTGGGAATGGCCATGAGCATGCAATCGCGTACACTGGAATATCCCAAGAGTTGTATGGCGCTAGGGCGACAATCAATGTTTGGACCCCTTCGATCGAACAAGTAAACGAGTTCAGTCTCTCACAGATTTGGGTTCTTTCCGGGTCGTTCGACGGCACAGATCTCAACAGCATAGAAGCTGGATGGCAGGTCAGTCCGGAGCTCTATGGTGACAGCAGACCTAGATTGTTCACTTACTGGACGACAGATTCATATCAAGCGACTGGGTGCTACAATCTTTTGTGTTCCGGATTTATCCAAACAAACAGCAGGATAGCCATTGGAGCTGCCATTTCCCCAGTCTCATCCTATGCCAGCAACCAATACGATGTCACCATTCTCATATGGAAGGATCCAAAGCTAGGAAATTGGTGGATGGGATTTGGAGACAATACCCTGGTAGGGTACTGGCCAGCAGAGCTATTTACACACTTGGCGGACAAGGCAACTATGGTGGAATGGGGCGGCGAGATTGTGAACTCGAGGGCCAACGGCGAGCACACCGCCACCCAAATGGGTTCGGGTCATTTCGCTGAAGACGGTTTTGGGAAAGCAAGCTATTTTCGAAACCTAGAGATTGTGGACTCGGACAACAGCCTCAGCTCGGTCCGGGATGTGTCAACTTTGGCTGAAAACACCAATTGCTACAACATCAAGAGCTCCTACAACAACGAATGGGGCACTCACTTCTACTATGGTGGCCCTGGGAGAAATGCACAGTGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

483

Amino Acids

53.84

Weight (kDa)

8.99

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 79 - 238 7.7e-39 Neprosin activation peptide
Neprosin PF03080 263 - 475 7.5e-89 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 859
AccB1I GGYRCC 1 cut(s) 337
AccII CGCG 2 cut(s) 660, 710
AccIII TCCGGA 2 cut(s) 877, 968
AciI CCGC 4 cut(s) 541, 1157, 1185, 1224
AclWI GGATC 5 cut(s) 306, 319, 413, 1067, 1080
AcoI YGGCCR 2 cut(s) 691, 1130
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 2 cut(s) 89, 1275
AfaI GTAC 4 cut(s) 480, 607, 713, 1127
AfiI CCNNNNNNNGG 5 cut(s) 334, 676, 1082, 1122, 1324
AflIII ACRYGT 1 cut(s) 658
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 2 cut(s) 208, 242
AhdI GACNNNNNGTC 1 cut(s) 513
AjnI CCWGG 2 cut(s) 1116, 1423
AjuI GAANNNNNNNTTGG 2 cut(s) 1248, 1280
Alw21I GWGCWC 4 cut(s) 548, 885, 1221, 1382
Alw26I GTCTC 4 cut(s) 175, 803, 1027, 1101
AlwI GGATC 5 cut(s) 306, 319, 413, 1067, 1080
Ama87I CYCGRG 1 cut(s) 1201
Aor13HI TCCGGA 2 cut(s) 877, 968
AoxI GGCC 5 cut(s) 347, 691, 1130, 1206, 1420
ApeKI GCWGC 4 cut(s) 125, 174, 484, 1007
ApoI RAATTY 1 cut(s) 103
AseI ATTAAT 2 cut(s) 429, 621
AsiGI ACCGGT 1 cut(s) 55
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 4 cut(s) 765, 1206, 1327, 1421
AsuC2I CCSGG 2 cut(s) 823, 1331
AsuHPI GGTGA 4 cut(s) 45, 293, 902, 1045
AsuII TTCGAA 1 cut(s) 1285
AvaI CYCGRG 1 cut(s) 1201
AvaII GGWCC 2 cut(s) 765, 1327
BaeGI GKGCMC 2 cut(s) 1403, 1445
BalI TGGCCA 2 cut(s) 693, 1132
BamHI GGATCC 1 cut(s) 1072
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 2 cut(s) 885, 1382
BbsI GAAGAC 1 cut(s) 1263
Bbv12I GWGCWC 4 cut(s) 548, 885, 1221, 1382
BbvCI CCTCAGC 2 cut(s) 563, 1319
BbvI GCAGC 4 cut(s) 112, 186, 496, 994
BccI CCATC 4 cut(s) 245, 395, 858, 1090
BceAI ACGGC 3 cut(s) 554, 850, 1228
BciT130I CCWGG 2 cut(s) 1118, 1425
BcnI CCSGG 2 cut(s) 823, 1331
BcoDI GTCTC 4 cut(s) 175, 803, 1027, 1101
BfaI CTAG 4 cut(s) 744, 903, 1082, 1292
BfmI CTRYAG 1 cut(s) 482
BfoI RGCGCY 1 cut(s) 744
BfuAI ACCTGC 1 cut(s) 859
BglII AGATCT 1 cut(s) 841
BisI GCNGC 5 cut(s) 126, 175, 485, 1008, 1186
BlsI GCNGC 5 cut(s) 127, 176, 486, 1009, 1187
Bme1390I CCNGG 4 cut(s) 823, 1118, 1331, 1425
Bme18I GGWCC 2 cut(s) 765, 1327
BmeRI GACNNNNNGTC 1 cut(s) 513
BmeT110I CYCGRG 1 cut(s) 1201
BmgT120I GGNCC 4 cut(s) 765, 1206, 1327, 1421
BmiI GGNNCC 4 cut(s) 339, 531, 767, 1074
BmrFI CCNGG 4 cut(s) 823, 1118, 1331, 1425
BmrI ACTGGG 2 cut(s) 954, 1013
BmsI GCATC 2 cut(s) 610, 655
BmuI ACTGGG 2 cut(s) 954, 1013
BpiI GAAGAC 1 cut(s) 1263
Bpu10I CCTNAGC 2 cut(s) 563, 1319
Bpu14I TTCGAA 1 cut(s) 1285
BpuEI CTTGAG 1 cut(s) 199
BpuMI CCSGG 2 cut(s) 823, 1331
BsaBI GATNNNNATC 1 cut(s) 933
BsaJI CCNNGG 3 cut(s) 1116, 1423, 1424
BsaWI WCCGGW 3 cut(s) 55, 877, 968
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 5 cut(s) 334, 676, 1082, 1122, 1324
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 7 cut(s) 189, 609, 721, 923, 949, 1019, 1133
Bse8I GATNNNNATC 1 cut(s) 933
BseAI TCCGGA 2 cut(s) 877, 968
BseBI CCWGG 2 cut(s) 1118, 1425
BseDI CCNNGG 3 cut(s) 1116, 1423, 1424
BseGI GGATG 5 cut(s) 559, 869, 1025, 1101, 1339
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 5 cut(s) 334, 676, 1082, 1122, 1324
BseMII CTCAG 2 cut(s) 554, 1333
BseNI ACTGG 7 cut(s) 189, 609, 721, 923, 949, 1019, 1133
BseRI GAGGAG 3 cut(s) 465, 468, 471
BseSI GKGCMC 2 cut(s) 1403, 1445
BseXI GCAGC 4 cut(s) 112, 186, 496, 994
Bsh1236I CGCG 2 cut(s) 660, 710
Bsh1285I CGRYCG 1 cut(s) 777
BshFI GGCC 5 cut(s) 349, 693, 1132, 1208, 1422
BshNI GGYRCC 1 cut(s) 337
BshTI ACCGGT 1 cut(s) 55
BsiEI CGRYCG 1 cut(s) 777
BsiHKAI GWGCWC 4 cut(s) 548, 885, 1221, 1382
BsiHKCI CYCGRG 1 cut(s) 1201
BsiSI CCGG 5 cut(s) 56, 822, 878, 969, 1330
BslFI GGGAC 2 cut(s) 19, 520
BslI CCNNNNNNNGG 5 cut(s) 334, 676, 1082, 1122, 1324
BsmAI GTCTC 4 cut(s) 175, 803, 1027, 1101
BsmFI GGGAC 2 cut(s) 19, 520
BsnI GGCC 5 cut(s) 349, 693, 1132, 1208, 1422
BsoBI CYCGRG 1 cut(s) 1201
Bsp119I TTCGAA 1 cut(s) 1285
Bsp1286I GDGCHC 6 cut(s) 548, 885, 1221, 1382, 1403, 1445
Bsp13I TCCGGA 2 cut(s) 877, 968
Bsp143I GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
BspACI CCGC 4 cut(s) 541, 1157, 1185, 1224
BspANI GGCC 5 cut(s) 349, 693, 1132, 1208, 1422
BspCNI CTCAG 2 cut(s) 555, 1332
BspEI TCCGGA 2 cut(s) 877, 968
BspFNI CGCG 2 cut(s) 660, 710
BspLI GGNNCC 4 cut(s) 339, 531, 767, 1074
BspMAI CTGCAG 1 cut(s) 486
BspMI ACCTGC 1 cut(s) 859
BspPI GGATC 5 cut(s) 306, 319, 413, 1067, 1080
BspT104I TTCGAA 1 cut(s) 1285
BspT107I GGYRCC 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 7 cut(s) 189, 609, 721, 923, 949, 1019, 1133
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 3 cut(s) 1116, 1423, 1424
BssMI GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
Bst2UI CCWGG 2 cut(s) 1118, 1425
Bst4CI ACNGT 3 cut(s) 529, 1262, 1440
BstBI TTCGAA 1 cut(s) 1285
BstC8I GCNNGC 7 cut(s) 225, 291, 702, 1036, 1134, 1217, 1276
BstDEI CTNAG 3 cut(s) 563, 570, 1319
BstF5I GGATG 5 cut(s) 559, 869, 1025, 1101, 1339
BstFNI CGCG 2 cut(s) 660, 710
BstH2I RGCGCY 1 cut(s) 744
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 8 cut(s) 216, 301, 327, 408, 646, 777, 844, 1075
BstMAI GTCTC 4 cut(s) 175, 803, 1027, 1101
BstMBI GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
BstMCI CGRYCG 1 cut(s) 777
BstMWI GCNNNNNNNGC 6 cut(s) 163, 493, 647, 1004, 1138, 1163
BstNI CCWGG 2 cut(s) 1118, 1425
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 4 cut(s) 821, 1116, 1329, 1423
BstSFI CTRYAG 1 cut(s) 482
BstSLI GKGCMC 2 cut(s) 1403, 1445
BstUI CGCG 2 cut(s) 660, 710
BstV1I GCAGC 4 cut(s) 112, 186, 496, 994
BstV2I GAAGAC 1 cut(s) 1263
BstX2I RGATCY 3 cut(s) 324, 841, 1072
BstXI CCANNNNNNTGG 1 cut(s) 1234
BstYI RGATCY 3 cut(s) 324, 841, 1072
BsuRI GGCC 5 cut(s) 349, 693, 1132, 1208, 1422
BtsCI GGATG 5 cut(s) 559, 869, 1025, 1101, 1339
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 4 cut(s) 435, 688, 714, 1445
BveI ACCTGC 1 cut(s) 859
Cac8I GCNNGC 7 cut(s) 225, 291, 702, 1036, 1134, 1217, 1276
CfoI GCGC 1 cut(s) 743
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 4 cut(s) 765, 1206, 1327, 1421
CpoI CGGWCCG 1 cut(s) 1327
CseI GACGC 2 cut(s) 649, 666
Csp6I GTAC 4 cut(s) 479, 606, 712, 1126
CspAI ACCGGT 1 cut(s) 55
CspCI CAANNNNNGTGG 2 cut(s) 1155, 1190
CspI CGGWCCG 1 cut(s) 1327
CviAII CATG 3 cut(s) 474, 695, 701
CviQI GTAC 4 cut(s) 479, 606, 712, 1126
DdeI CTNAG 3 cut(s) 563, 570, 1319
DpnI GATC 8 cut(s) 215, 300, 326, 407, 645, 776, 843, 1074
DpnII GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
DraI TTTAAA 1 cut(s) 312
DriI GACNNNNNGTC 1 cut(s) 513
EaeI YGGCCR 2 cut(s) 691, 1130
Eam1105I GACNNNNNGTC 1 cut(s) 513
EciI GGCGGA 2 cut(s) 556, 1172
Ecl136II GAGCTC 2 cut(s) 883, 1380
Eco24I GRGCYC 2 cut(s) 885, 1382
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 765, 1327
Eco53kI GAGCTC 2 cut(s) 883, 1380
Eco57I CTGAAG 2 cut(s) 89, 1275
Eco88I CYCGRG 1 cut(s) 1201
EcoICRI GAGCTC 2 cut(s) 883, 1380
EcoRII CCWGG 2 cut(s) 1116, 1423
EcoRV GATATC 1 cut(s) 259
EcoT38I GRGCYC 2 cut(s) 885, 1382
FaeI CATG 3 cut(s) 477, 698, 704
FaqI GGGAC 2 cut(s) 19, 520
FatI CATG 3 cut(s) 473, 694, 700
FauNDI CATATG 1 cut(s) 1064
Fnu4HI GCNGC 5 cut(s) 126, 175, 485, 1008, 1186
FokI GGATG 5 cut(s) 566, 876, 1012, 1108, 1346
FriOI GRGCYC 2 cut(s) 885, 1382
Fsp4HI GCNGC 5 cut(s) 126, 175, 485, 1008, 1186
FspBI CTAG 4 cut(s) 744, 903, 1082, 1292
GlaI GCGC 1 cut(s) 742
GluI GCNGC 5 cut(s) 126, 175, 485, 1008, 1186
HaeII RGCGCY 1 cut(s) 744
HaeIII GGCC 5 cut(s) 349, 693, 1132, 1208, 1422
HapII CCGG 5 cut(s) 56, 822, 878, 969, 1330
HgaI GACGC 2 cut(s) 649, 666
HhaI GCGC 1 cut(s) 743
Hin1II CATG 3 cut(s) 477, 698, 704
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HincII GTYRAC 1 cut(s) 1341
HindII GTYRAC 1 cut(s) 1341
HinfI GANTC 3 cut(s) 316, 929, 1304
HpaII CCGG 5 cut(s) 56, 822, 878, 969, 1330
HphI GGTGA 4 cut(s) 45, 293, 902, 1045
Hpy166II GTNNAC 6 cut(s) 714, 787, 912, 1198, 1303, 1341
Hpy188I TCNGA 3 cut(s) 69, 519, 1309
Hpy188III TCNNGA 7 cut(s) 135, 248, 328, 654, 878, 969, 1375
Hpy8I GTNNAC 6 cut(s) 714, 787, 912, 1198, 1303, 1341
Hpy99I CGWCG 2 cut(s) 665, 836
HpyAV CCTTC 4 cut(s) 21, 328, 780, 1063
HpyCH4III ACNGT 3 cut(s) 529, 1262, 1440
HpyCH4V TGCA 4 cut(s) 473, 484, 704, 1436
HpyF10VI GCNNNNNNNGC 6 cut(s) 163, 493, 647, 1004, 1138, 1163
HpyF3I CTNAG 3 cut(s) 563, 570, 1319
Hsp92II CATG 3 cut(s) 477, 698, 704
HspAI GCGC 1 cut(s) 741
Kpn2I TCCGGA 2 cut(s) 877, 968
Kzo9I GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
LmnI GCTCC 4 cut(s) 543, 880, 1004, 1385
Lsp1109I GCAGC 4 cut(s) 112, 186, 496, 994
LweI GCATC 2 cut(s) 610, 655
MaeI CTAG 4 cut(s) 744, 903, 1082, 1292
MaeIII GTNAC 4 cut(s) 51, 361, 890, 1051
MalI GATC 8 cut(s) 215, 300, 326, 407, 645, 776, 843, 1074
MboI GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
MboII GAAGA 4 cut(s) 53, 56, 382, 1268
MfeI CAATTG 1 cut(s) 1360
MflI RGATCY 3 cut(s) 324, 841, 1072
MhlI GDGCHC 6 cut(s) 548, 885, 1221, 1382, 1403, 1445
MlsI TGGCCA 2 cut(s) 693, 1132
MluCI AATT 4 cut(s) 103, 395, 1087, 1360
MluI ACGCGT 1 cut(s) 658
MluNI TGGCCA 2 cut(s) 693, 1132
MlyI GAGTC 1 cut(s) 1298
MnlI CCTC 9 cut(s) 315, 324, 443, 446, 449, 515, 558, 1197, 1328
Mox20I TGGCCA 2 cut(s) 693, 1132
MroI TCCGGA 2 cut(s) 877, 968
MscI TGGCCA 2 cut(s) 693, 1132
MseI TTAA 5 cut(s) 311, 429, 465, 615, 621
MslI CAYNNNNRTG 3 cut(s) 501, 699, 1232
Msp20I TGGCCA 2 cut(s) 693, 1132
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 5 cut(s) 56, 822, 878, 969, 1330
MspR9I CCNGG 4 cut(s) 823, 1118, 1331, 1425
MunI CAATTG 1 cut(s) 1360
MvaI CCWGG 2 cut(s) 1118, 1425
MvnI CGCG 2 cut(s) 660, 710
MwoI GCNNNNNNNGC 6 cut(s) 163, 493, 647, 1004, 1138, 1163
NciI CCSGG 2 cut(s) 823, 1331
NdeI CATATG 1 cut(s) 1064
NdeII GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
NlaIII CATG 3 cut(s) 477, 698, 704
NlaIV GGNNCC 4 cut(s) 339, 531, 767, 1074
NmuCI GTSAC 3 cut(s) 51, 890, 1051
NspI RCATGY 1 cut(s) 704
NspV TTCGAA 1 cut(s) 1285
PaeI GCATGC 1 cut(s) 704
PaeR7I CTCGAG 1 cut(s) 1201
PasI CCCWGGG 1 cut(s) 1424
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PfeI GAWTC 2 cut(s) 316, 929
PfoI TCCNGGA 1 cut(s) 1329
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 5 cut(s) 127, 176, 486, 1009, 1187
Ple19I CGATCG 1 cut(s) 777
PleI GAGTC 1 cut(s) 1298
PpsI GAGTC 1 cut(s) 1298
PshBI ATTAAT 2 cut(s) 429, 621
Psp124BI GAGCTC 2 cut(s) 885, 1382
Psp6I CCWGG 2 cut(s) 1116, 1423
PspGI CCWGG 2 cut(s) 1116, 1423
PspN4I GGNNCC 4 cut(s) 339, 531, 767, 1074
PspPI GGNCC 4 cut(s) 765, 1206, 1327, 1421
PspXI VCTCGAGB 1 cut(s) 1201
PstI CTGCAG 1 cut(s) 486
PsuI RGATCY 3 cut(s) 324, 841, 1072
PvuI CGATCG 1 cut(s) 777
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 4 cut(s) 480, 607, 713, 1127
RsaNI GTAC 4 cut(s) 479, 606, 712, 1126
RseI CAYNNNNRTG 3 cut(s) 501, 699, 1232
Rsr2I CGGWCCG 1 cut(s) 1327
RsrII CGGWCCG 1 cut(s) 1327
SacI GAGCTC 2 cut(s) 885, 1382
SaqAI TTAA 5 cut(s) 311, 429, 465, 615, 621
SatI GCNGC 5 cut(s) 126, 175, 485, 1008, 1186
Sau3AI GATC 8 cut(s) 213, 298, 324, 405, 643, 774, 841, 1072
Sau96I GGNCC 4 cut(s) 765, 1206, 1327, 1421
SchI GAGTC 1 cut(s) 1298
ScrFI CCNGG 4 cut(s) 823, 1118, 1331, 1425
SduI GDGCHC 6 cut(s) 548, 885, 1221, 1382, 1403, 1445
SfaNI GCATC 2 cut(s) 610, 655
SfcI CTRYAG 1 cut(s) 482
Sfr274I CTCGAG 1 cut(s) 1201
SfuI TTCGAA 1 cut(s) 1285
SinI GGWCC 2 cut(s) 765, 1327
SlaI CTCGAG 1 cut(s) 1201
SmiMI CAYNNNNRTG 3 cut(s) 501, 699, 1232
SmlI CTYRAG 2 cut(s) 178, 1201
SmoI CTYRAG 2 cut(s) 178, 1201
SphI GCATGC 1 cut(s) 704
Sse9I AATT 4 cut(s) 103, 395, 1087, 1360
SsiI CCGC 4 cut(s) 541, 1157, 1185, 1224
SspI AATATT 2 cut(s) 619, 634
SspMI CTAG 4 cut(s) 744, 903, 1082, 1292
SstI GAGCTC 2 cut(s) 885, 1382
StyD4I CCNGG 4 cut(s) 821, 1116, 1329, 1423
TaaI ACNGT 3 cut(s) 529, 1262, 1440
TaqI TCGA 6 cut(s) 663, 773, 777, 831, 1202, 1285
TaqII GACCGA 1 cut(s) 1315
TasI AATT 4 cut(s) 103, 395, 1087, 1360
TauI GCSGC 1 cut(s) 1188
TfiI GAWTC 2 cut(s) 316, 929
Tru1I TTAA 5 cut(s) 311, 429, 465, 615, 621
Tru9I TTAA 5 cut(s) 311, 429, 465, 615, 621
TscAI CASTG 4 cut(s) 442, 688, 721, 1445
TseFI GTSAC 3 cut(s) 51, 890, 1051
TseI GCWGC 4 cut(s) 125, 174, 484, 1007
Tsp45I GTSAC 3 cut(s) 51, 890, 1051
TspDTI ATGAA 3 cut(s) 263, 389, 921
TspRI CASTG 4 cut(s) 442, 688, 721, 1445
VpaK11BI GGWCC 2 cut(s) 765, 1327
VspI ATTAAT 2 cut(s) 429, 621
XapI RAATTY 1 cut(s) 103
XceI RCATGY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 734
XhoI CTCGAG 1 cut(s) 1201
XspI CTAG 4 cut(s) 744, 903, 1082, 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.