pycom04g20750

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013071
Physical Location & Seq
Reverse (-)
22173413 .. 22177226
3814 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20750.2

Sequence Viewer

Length: 1077 bp
ATGAAGCAAAATAATGATGACGAATTAGATGGGATTAAGGATAATAATAATGTGATTAATGCCACTGCTAATCAAGGAGGAGGAGGAGTGATTAAGGGTGCATGGGTACTGCAGCAAATGTGGCATAGAAATGGGACAAGATGTCCGAGAGGAACGGTTCCTATACGGCGGAGCACAACGGATGATGTGCTGAGGGCCACGTCTTTGTTTGACTTTGGGAAGAAACAGCATCGTACCAGTTTTAATATTAATAATAAGAATATTACTGCTGATCTTGCTTCCAGACGCGTCGATGCCCCCGATGTGGTCAGTGGGAATGGCCATGAGCATGCAATCGCGTACACTGGAACATCCCAAGAGTTATATGGAGCTAGGGCTACAATCAATGTTTGGACCCCTTCGATCGAACAGGTAAACGAGTTCAGTCTCTCACAGATTTGGGTTCTTTCCGGGTCGTTCGACGGCACAGATCTCAACAGCATAGAAGCTGGATGGCAGGTCAGTCCGGAGCTCTATGGTGACAGCAGACCTAGATTGTTCACTTACTGGACGACAGATTCATATCAAGCAACTGGGTGCTACAATCTTCTGTGTTCCGGATTTATCCAAACAAACAGCAGGATAGCTATTGGAGCTTCCATTTCCCCAGTCTCATCCTATGCCAGCAACCAATACGATGTCACCATTCTTATATGGAAGGATCCAAAGCTAGGAAATTGGTGGATGGGATTTGGAGACAATACCCTGGTAGGGTACTGGCCAGCAGAGCTATTTACACACTTGGCGGACAAGGCAACTATGGTGGAATGGGGCGGCGAGATTGTGAACTCGAGGGCCAACGGCGAGCACACCGCCACCCAAATGGGTTCGGGTCATTTCGCTGAAGACGGGTTTGGGAAAGCAAGCTATTTTCGAAACCTAGAGATTGTGGACTCGGACAACAGCCTCAGCTCGGTCCGGGATGTGTCAACTTTGGCTGAAAATACCAATTGCTACGACATCAAGAGCTCCTACAACAATGAATGGGGCACTCACTTCTACTATGGTGGCCCTGGGAGAAATGCACAATGCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

359

Amino Acids

39.57

Weight (kDa)

5.27

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18460
fragaria_vesca FvH4_6g01420 FvH4_6g01420
malus_domestica MD04G1233600.v1.1
prunus_persica Prupe.6G353500_v2.0.a1 Prupe.6G353500_v2.0.a1
pyrus_communis pycom04g20750
rosa_chinensis RchiOBHm_Chr3g0449051
rosa_laevigata RLG00000025858
rosa_multiflora Rmu_co8511853.1_g000001 Rmu_sc0003116.1_g000008
rosa_roxburghii Rroxscaffold_6G00427160
rosa_rugosa Rorug02G0617700
rosa_samantha Rh3AG016100 Rh3BG016300 Rh3CG015300 Rh3DG016600
rosa_wichuraiana Rw3G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 487
AccII CGCG 2 cut(s) 288, 338
AccIII TCCGGA 2 cut(s) 505, 596
AciI CCGC 4 cut(s) 169, 785, 813, 852
AclWI GGATC 2 cut(s) 695, 708
AcoI YGGCCR 2 cut(s) 319, 758
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 4 cut(s) 108, 235, 341, 755
AfiI CCNNNNNNNGG 4 cut(s) 304, 710, 750, 952
AflIII ACRYGT 1 cut(s) 286
AhdI GACNNNNNGTC 1 cut(s) 141
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 2 cut(s) 744, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 876, 908
Alw21I GWGCWC 4 cut(s) 176, 513, 849, 1010
Alw26I GTCTC 3 cut(s) 431, 655, 729
AlwI GGATC 2 cut(s) 695, 708
Ama87I CYCGRG 1 cut(s) 829
Aor13HI TCCGGA 2 cut(s) 505, 596
AoxI GGCC 5 cut(s) 195, 319, 758, 834, 1048
ApeKI GCWGC 1 cut(s) 112
AseI ATTAAT 2 cut(s) 57, 249
AspS9I GGNCC 5 cut(s) 195, 393, 834, 955, 1049
AsuC2I CCSGG 2 cut(s) 451, 959
AsuHPI GGTGA 2 cut(s) 530, 673
AsuII TTCGAA 1 cut(s) 913
AvaI CYCGRG 1 cut(s) 829
AvaII GGWCC 2 cut(s) 393, 955
BaeGI GKGCMC 1 cut(s) 1031
BalI TGGCCA 2 cut(s) 321, 760
BamHI GGATCC 1 cut(s) 700
BanII GRGCYC 2 cut(s) 513, 1010
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 4 cut(s) 176, 513, 849, 1010
BbvCI CCTCAGC 2 cut(s) 191, 947
BbvI GCAGC 1 cut(s) 124
BccI CCATC 3 cut(s) 23, 486, 718
BceAI ACGGC 3 cut(s) 182, 478, 856
BciT130I CCWGG 2 cut(s) 746, 1053
BcnI CCSGG 2 cut(s) 451, 959
BcoDI GTCTC 3 cut(s) 431, 655, 729
BfaI CTAG 4 cut(s) 372, 531, 710, 920
BfmI CTRYAG 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 487
BglII AGATCT 1 cut(s) 469
BisI GCNGC 2 cut(s) 113, 814
BlsI GCNGC 2 cut(s) 114, 815
Bme1390I CCNGG 4 cut(s) 451, 746, 959, 1053
Bme18I GGWCC 2 cut(s) 393, 955
BmeRI GACNNNNNGTC 1 cut(s) 141
BmeT110I CYCGRG 1 cut(s) 829
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 5 cut(s) 195, 393, 834, 955, 1049
BmiI GGNNCC 3 cut(s) 159, 395, 702
BmrFI CCNGG 4 cut(s) 451, 746, 959, 1053
BmrI ACTGGG 2 cut(s) 582, 641
BmsI GCATC 2 cut(s) 238, 283
BmuI ACTGGG 2 cut(s) 582, 641
BpiI GAAGAC 1 cut(s) 891
Bpu10I CCTNAGC 2 cut(s) 191, 947
Bpu14I TTCGAA 1 cut(s) 913
BpuMI CCSGG 2 cut(s) 451, 959
BsaBI GATNNNNATC 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 744, 1051, 1052
BsaWI WCCGGW 2 cut(s) 505, 596
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 4 cut(s) 304, 710, 750, 952
Bse1I ACTGG 6 cut(s) 237, 349, 551, 577, 647, 761
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 2 cut(s) 505, 596
BseBI CCWGG 2 cut(s) 746, 1053
BseDI CCNNGG 3 cut(s) 744, 1051, 1052
BseGI GGATG 6 cut(s) 187, 350, 497, 653, 729, 967
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 4 cut(s) 304, 710, 750, 952
BseMII CTCAG 2 cut(s) 182, 961
BseNI ACTGG 6 cut(s) 237, 349, 551, 577, 647, 761
BseRI GAGGAG 3 cut(s) 93, 96, 99
BseSI GKGCMC 1 cut(s) 1031
BseXI GCAGC 1 cut(s) 124
Bsh1236I CGCG 2 cut(s) 288, 338
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 5 cut(s) 197, 321, 760, 836, 1050
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 4 cut(s) 176, 513, 849, 1010
BsiHKCI CYCGRG 1 cut(s) 829
BsiSI CCGG 4 cut(s) 450, 506, 597, 958
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 4 cut(s) 304, 710, 750, 952
BsmAI GTCTC 3 cut(s) 431, 655, 729
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 5 cut(s) 197, 321, 760, 836, 1050
BsoBI CYCGRG 1 cut(s) 829
Bsp119I TTCGAA 1 cut(s) 913
Bsp1286I GDGCHC 5 cut(s) 176, 513, 849, 1010, 1031
Bsp13I TCCGGA 2 cut(s) 505, 596
Bsp143I GATC 4 cut(s) 271, 402, 469, 700
BspACI CCGC 4 cut(s) 169, 785, 813, 852
BspANI GGCC 5 cut(s) 197, 321, 760, 836, 1050
BspCNI CTCAG 2 cut(s) 183, 960
BspEI TCCGGA 2 cut(s) 505, 596
BspFNI CGCG 2 cut(s) 288, 338
BspLI GGNNCC 3 cut(s) 159, 395, 702
BspMAI CTGCAG 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 2 cut(s) 695, 708
BspT104I TTCGAA 1 cut(s) 913
BsrI ACTGG 6 cut(s) 237, 349, 551, 577, 647, 761
BssECI CCNNGG 3 cut(s) 744, 1051, 1052
BssMI GATC 4 cut(s) 271, 402, 469, 700
Bst2UI CCWGG 2 cut(s) 746, 1053
Bst4CI ACNGT 1 cut(s) 157
BstBI TTCGAA 1 cut(s) 913
BstC8I GCNNGC 5 cut(s) 330, 664, 762, 845, 904
BstDEI CTNAG 2 cut(s) 191, 947
BstF5I GGATG 6 cut(s) 187, 350, 497, 653, 729, 967
BstFNI CGCG 2 cut(s) 288, 338
BstKTI GATC 4 cut(s) 274, 405, 472, 703
BstMAI GTCTC 3 cut(s) 431, 655, 729
BstMBI GATC 4 cut(s) 271, 402, 469, 700
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 5 cut(s) 121, 275, 632, 766, 791
BstNI CCWGG 2 cut(s) 746, 1053
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 4 cut(s) 449, 744, 957, 1051
BstSFI CTRYAG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 1031
BstUI CGCG 2 cut(s) 288, 338
BstV1I GCAGC 1 cut(s) 124
BstV2I GAAGAC 1 cut(s) 891
BstX2I RGATCY 2 cut(s) 469, 700
BstXI CCANNNNNNTGG 1 cut(s) 862
BstYI RGATCY 2 cut(s) 469, 700
BsuRI GGCC 5 cut(s) 197, 321, 760, 836, 1050
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 6 cut(s) 187, 350, 497, 653, 729, 967
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 3 cut(s) 63, 316, 342
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 5 cut(s) 330, 664, 762, 845, 904
Cfr13I GGNCC 5 cut(s) 195, 393, 834, 955, 1049
CpoI CGGWCCG 1 cut(s) 955
CseI GACGC 2 cut(s) 277, 294
Csp6I GTAC 4 cut(s) 107, 234, 340, 754
CspCI CAANNNNNGTGG 2 cut(s) 783, 818
CspI CGGWCCG 1 cut(s) 955
CviAII CATG 4 cut(s) 102, 323, 329, 1074
CviQI GTAC 4 cut(s) 107, 234, 340, 754
DdeI CTNAG 2 cut(s) 191, 947
DpnI GATC 4 cut(s) 273, 404, 471, 702
DpnII GATC 4 cut(s) 271, 402, 469, 700
DriI GACNNNNNGTC 1 cut(s) 141
EaeI YGGCCR 2 cut(s) 319, 758
Eam1105I GACNNNNNGTC 1 cut(s) 141
EciI GGCGGA 2 cut(s) 184, 800
Ecl136II GAGCTC 2 cut(s) 511, 1008
Eco24I GRGCYC 2 cut(s) 513, 1010
Eco47I GGWCC 2 cut(s) 393, 955
Eco53kI GAGCTC 2 cut(s) 511, 1008
Eco57I CTGAAG 1 cut(s) 903
Eco88I CYCGRG 1 cut(s) 829
EcoICRI GAGCTC 2 cut(s) 511, 1008
EcoRII CCWGG 2 cut(s) 744, 1051
EcoT38I GRGCYC 2 cut(s) 513, 1010
FaeI CATG 4 cut(s) 105, 326, 332, 1077
FaqI GGGAC 1 cut(s) 148
FatI CATG 4 cut(s) 101, 322, 328, 1073
Fnu4HI GCNGC 2 cut(s) 113, 814
FokI GGATG 6 cut(s) 194, 337, 504, 640, 736, 974
FriOI GRGCYC 2 cut(s) 513, 1010
Fsp4HI GCNGC 2 cut(s) 113, 814
FspBI CTAG 4 cut(s) 372, 531, 710, 920
GluI GCNGC 2 cut(s) 113, 814
HaeIII GGCC 5 cut(s) 197, 321, 760, 836, 1050
HapII CCGG 4 cut(s) 450, 506, 597, 958
HgaI GACGC 2 cut(s) 277, 294
Hin1II CATG 4 cut(s) 105, 326, 332, 1077
HincII GTYRAC 1 cut(s) 969
HindII GTYRAC 1 cut(s) 969
HinfI GANTC 2 cut(s) 557, 932
HpaII CCGG 4 cut(s) 450, 506, 597, 958
HphI GGTGA 2 cut(s) 530, 673
Hpy166II GTNNAC 6 cut(s) 342, 415, 540, 826, 931, 969
Hpy188I TCNGA 2 cut(s) 147, 937
Hpy188III TCNNGA 4 cut(s) 282, 506, 597, 1003
Hpy8I GTNNAC 6 cut(s) 342, 415, 540, 826, 931, 969
Hpy99I CGWCG 2 cut(s) 293, 464
HpyAV CCTTC 2 cut(s) 408, 691
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 4 cut(s) 101, 112, 332, 1064
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 275, 632, 766, 791
HpyF3I CTNAG 2 cut(s) 191, 947
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 4 cut(s) 105, 326, 332, 1077
Kpn2I TCCGGA 2 cut(s) 505, 596
Kzo9I GATC 4 cut(s) 271, 402, 469, 700
LmnI GCTCC 5 cut(s) 171, 368, 508, 632, 1013
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 2 cut(s) 238, 283
MaeI CTAG 4 cut(s) 372, 531, 710, 920
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 518, 679
MalI GATC 4 cut(s) 273, 404, 471, 702
MboI GATC 4 cut(s) 271, 402, 469, 700
MboII GAAGA 3 cut(s) 232, 578, 896
MfeI CAATTG 1 cut(s) 988
MflI RGATCY 2 cut(s) 469, 700
MhlI GDGCHC 5 cut(s) 176, 513, 849, 1010, 1031
MlsI TGGCCA 2 cut(s) 321, 760
MluCI AATT 3 cut(s) 23, 715, 988
MluI ACGCGT 1 cut(s) 286
MluNI TGGCCA 2 cut(s) 321, 760
MlyI GAGTC 1 cut(s) 926
MnlI CCTC 7 cut(s) 71, 74, 77, 143, 186, 825, 956
Mox20I TGGCCA 2 cut(s) 321, 760
MroI TCCGGA 2 cut(s) 505, 596
MscI TGGCCA 2 cut(s) 321, 760
MseI TTAA 5 cut(s) 36, 57, 93, 243, 249
MslI CAYNNNNRTG 3 cut(s) 129, 327, 860
Msp20I TGGCCA 2 cut(s) 321, 760
MspI CCGG 4 cut(s) 450, 506, 597, 958
MspR9I CCNGG 4 cut(s) 451, 746, 959, 1053
MunI CAATTG 1 cut(s) 988
MvaI CCWGG 2 cut(s) 746, 1053
MvnI CGCG 2 cut(s) 288, 338
MwoI GCNNNNNNNGC 5 cut(s) 121, 275, 632, 766, 791
NciI CCSGG 2 cut(s) 451, 959
NdeII GATC 4 cut(s) 271, 402, 469, 700
NlaIII CATG 4 cut(s) 105, 326, 332, 1077
NlaIV GGNNCC 3 cut(s) 159, 395, 702
NmuCI GTSAC 2 cut(s) 518, 679
NspI RCATGY 1 cut(s) 332
NspV TTCGAA 1 cut(s) 913
PaeI GCATGC 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 829
PasI CCCWGGG 1 cut(s) 1052
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 1 cut(s) 557
PfoI TCCNGGA 1 cut(s) 957
PkrI GCNGC 2 cut(s) 114, 815
Ple19I CGATCG 1 cut(s) 405
PleI GAGTC 1 cut(s) 926
PpsI GAGTC 1 cut(s) 926
PshBI ATTAAT 2 cut(s) 57, 249
Psp124BI GAGCTC 2 cut(s) 513, 1010
Psp6I CCWGG 2 cut(s) 744, 1051
PspGI CCWGG 2 cut(s) 744, 1051
PspN4I GGNNCC 3 cut(s) 159, 395, 702
PspPI GGNCC 5 cut(s) 195, 393, 834, 955, 1049
PspXI VCTCGAGB 1 cut(s) 829
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 2 cut(s) 469, 700
PvuI CGATCG 1 cut(s) 405
RsaI GTAC 4 cut(s) 108, 235, 341, 755
RsaNI GTAC 4 cut(s) 107, 234, 340, 754
RseI CAYNNNNRTG 3 cut(s) 129, 327, 860
Rsr2I CGGWCCG 1 cut(s) 955
RsrII CGGWCCG 1 cut(s) 955
SacI GAGCTC 2 cut(s) 513, 1010
SaqAI TTAA 5 cut(s) 36, 57, 93, 243, 249
SatI GCNGC 2 cut(s) 113, 814
Sau3AI GATC 4 cut(s) 271, 402, 469, 700
Sau96I GGNCC 5 cut(s) 195, 393, 834, 955, 1049
SchI GAGTC 1 cut(s) 926
ScrFI CCNGG 4 cut(s) 451, 746, 959, 1053
SduI GDGCHC 5 cut(s) 176, 513, 849, 1010, 1031
SfaNI GCATC 2 cut(s) 238, 283
SfcI CTRYAG 1 cut(s) 110
Sfr274I CTCGAG 1 cut(s) 829
SfuI TTCGAA 1 cut(s) 913
SinI GGWCC 2 cut(s) 393, 955
SlaI CTCGAG 1 cut(s) 829
SmiMI CAYNNNNRTG 3 cut(s) 129, 327, 860
SmlI CTYRAG 1 cut(s) 829
SmoI CTYRAG 1 cut(s) 829
SphI GCATGC 1 cut(s) 332
Sse9I AATT 3 cut(s) 23, 715, 988
SsiI CCGC 4 cut(s) 169, 785, 813, 852
SspI AATATT 2 cut(s) 247, 262
SspMI CTAG 4 cut(s) 372, 531, 710, 920
SstI GAGCTC 2 cut(s) 513, 1010
StyD4I CCNGG 4 cut(s) 449, 744, 957, 1051
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 1 cut(s) 203
TaqI TCGA 6 cut(s) 291, 401, 405, 459, 830, 913
TaqII GACCGA 1 cut(s) 943
TasI AATT 3 cut(s) 23, 715, 988
TauI GCSGC 1 cut(s) 816
TfiI GAWTC 1 cut(s) 557
Tru1I TTAA 5 cut(s) 36, 57, 93, 243, 249
Tru9I TTAA 5 cut(s) 36, 57, 93, 243, 249
TscAI CASTG 3 cut(s) 70, 316, 349
TseFI GTSAC 2 cut(s) 518, 679
TseI GCWGC 1 cut(s) 112
Tsp45I GTSAC 2 cut(s) 518, 679
TspDTI ATGAA 3 cut(s) 17, 549, 1035
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 3 cut(s) 70, 316, 349
VpaK11BI GGWCC 2 cut(s) 393, 955
VspI ATTAAT 2 cut(s) 57, 249
XceI RCATGY 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 362
XhoI CTCGAG 1 cut(s) 829
XspI CTAG 4 cut(s) 372, 531, 710, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.