AT5G18700

ATP binding binding kinase protein kinase protein serine threonine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011033
Physical Location & Seq
Reverse (-)
6235032 .. 6240916
5885 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18700.1

Sequence Viewer

Length: 4101 bp
ATGAATCAGTACCACATCTACGAAGCTATTGGACATGGCAAATGCTCGACTGTGTATAAAGGAAGGAAGAAGAAGACGATCGAGTATTTTGCCTGCAAGAGTGTCGATAAGTCGCGGAAGAACAAGGTTCTTCAAGAGGTCAGGATCCTTCACTCGCTAAATCATCCAAACGTACTCAAGTTCTATGCTTGGTATGAAACTTCTGCTCACATGTGGTTAGTTCTGGAATATTGCGTTGGTGGTGATCTCAGGACATTATTGCAGCAGGACTGTAAACTGCCAGAAGAATCTATCTATGGTCTTGCTTATGATCTTGTCATAGCCCTGCAGTATTTACATTCGAAAGGAATTATATATTGTGATCTGAAACCATCAAACATCTTACTTGATGAAAATGGGCATATTAAGCTGTGTGATTTTGGTTTGTCAAGGAAGCTGGATGATATTTCTAAATCTCCTTCCACGGGAAAACGTGGTACCCCGTATTATATGGCTCCAGAGCTATATGAAGATGGAGGGATCCATTCTTTTGCTTCTGATCTGTGGGCACTTGGTTGTGTACTATATGAATGTTACACAGGAAGACCTCCTTTTGTGGCAAGAGAGTTTACCCAGCTTGTGAAATCTATTCATTCAGATCCAACTCCTCCACTCCCTGGAAATGCTAGTCGTTCATTTGTCAATCTCATTGAGTCTCTTCTTATCAAAGACCCAGCTCAAAGAATACAATGGGCAGATCTCTGTGGTCATGCATTTTGGAAGAGTAAGATAAATTTAGTTCAGTTGCCTACTCAGCCTGCTTTTGATGATATGATTGGCATAAACACAAAGCCATGCCTTTCCGAGCGTAATGGAGATAGACCAAATAAGACCCCTCCAAAGTACCGGGAGAAAGATCGAAAAGGGGGTTCAAAGCAAAATGAGAACTCCATTCAAGGTTCAAAAGGCCATGAGACGCCGATAAAGGGCACCCCTGGTGGGTCTAAAGCTCAAGCAAAGCTTCCTAGTAGAGCAACTGAGGAAAAGCATGGAGGTCGTCCTGCTGCTAACAGACAGGTGAATATTCTAAGATTGTCAAGGATAGCAAAGGCAAATCTTCAAAAGGAAAATGAGAAGGAAAACTATAGGAGACCCTTACCTAATAGCAACGAGAATTGTGCAGAAGTGAAGATCGACAACACTGATATGGAACTTGATTTTGATGAAAACAATGATGATGAGGGACCTGATGAATCAGAAGGAACCGAAAACACCTCTTGTGCACAAGAAGAAAGAGTAATGAGCCATAATGAGAATCACAGAAGACAAAGAGTTGTAAGCAGTAATGTGCCTGATGAGAACTCGTCTGCAAATGAAACACCAACCTTGGGTGAAGCAAGAGATTGCCATGAAGATCAGTCAGAGCCAATGGACATGTCAGCTGCCCCACCTAGTGCTAGTCCTCAACTTAAAACTCACCGAGGAAGAGAAACTTCTGGGGTTGCTGTTAATCATGACTCATCTAAAGCACCTACTAGCCTCACTGACGTCTTTTGGCATATATCTGATCTTTCTGTTAGGCCTGTCATGCCCAGCAGAAAATCTGACAAAGAAGCTGTGCATTCTCTCTCATTCGAAACACCACAGCCCTCTGATTTTAGTAAGAAGGGAAAGCAAGAATTAGAACCGCTGAACAATAGGATCATAACAGTTCTAAGTGGGAGCAGTTCTGGACTGTCAGAGAAACAGAATTTGATCAGATACCTCGAGACATTGAGTACCAATGCTGATGCAGCCAACATCCTGACTAACGGACCAATAATGCTTGTGCTTGTTAAAGTTCTGAGATTATCCAAGACCCCAGCATTTCGTGTACAAATTGCTTCACTAATTGGTTTGTTAATTCGGCATTCTACTTCAATCGAAGATGACTTGGCAAATTCTGGTATTTTAGACTCTCTTACTAATGGCCTCAGAGACAAGCATGAGAAAGTGAGAAGATTCTCTATGGCAGCTCTAGGTGAATTGCTATTCTACATCTCAACTCAAAATGAGCATAAGGATTTCAAACCACCAGAATCCCCATCAAAAGAAACCCGGTCTGCATCGGGTTGGCAGGTTTCAAATGCCTTGATCTCTCTTGTATCATCTGTCTTACGGAAGGGAGAAGATGACCTGACTCAGGTTTATGCGTTAAGGACAATAGAAAACATCTGCAGTCAAGGAGCTTACTGGGCCACCCGTTTCTCCAGCCAGGATTTGATTAGCAACCTCTGCTATATCTACAAGGCAACGGGGAAACAGGAGAGCATGAGGCAAACTGCTGGATCCTGTTTAGTCCGTCTTGCTCGCTTTAACCCTCCTTGCATTCAGACGGTCGTGGAAAAGCTTTCATTGAAGGAAATAGCTTCATCTTTTGTCAAAGGCAGCGCAAGAGAGCAGCAAGTCTGCTTAAATCTTCTGAACATGGCTATGATTGGAAGTCATACATTCACAAGCTTTGGAAGGCATCTCGTGACATTGACAGAGGAGAAGAACCTTTTCCCTAGTTTACTCTCCATCATAGAGCAAGGAACAGAAGTTCTGAGGGGCAAAGCTCTTCTCTTTGTTGCATTTCTCTGCAAAAACAGTAGGCGTTGGCTGACAAATTTCTTCTGCAATGCAAGATTTCTTCCTGTGGTGGACAGATTAGCTAAAGAAAAAGACAGTTACCTGCAACAATGTCTTGAGGCATTTGTGAATGTAATCGCCTCCATAATACCGGGCATGCTGGATACAATAACCAATGATATCCAACAACTGATGACAGGAAGACGCCATGGACCTGTCTCTCCCCTTAATAGTCGAGCTCCTGTAAAGACTAATGCACACCTGTTCCCTGTGGTTCTTCACCTTCTGGGAAGCTCTTCGTTTAAGAACAAGATGGTAACTCCACAAGTCCTAAGGCAGTTGGCAAATCTTACAAAACTTGTGGAAGCATCGTTTCAGGGAAGAGATGACTTCCGTGTAACCCTTCTTCAAGTTCTAGAGTGCATAACAGGAGATGCGCCTCTGGTTACACAAAATGGTGAGATTATTATCCGTGAGATTCTCCCATCTCTGGCTGCAATTTACAATGGAAACAAAGACGGTGATGCTCGGTTTCTCTGTTTGAAAATCTGGTTCGATTCATTGACCATTCTCTTAACTGAGTGCACCGAAATCGAGCAACAAATATCGGAGGATTTGAAATCAATATCCAACTCTCATTTCCTCCCACTCTACCCTGCACTGATCCAAGACGAAGATCCAATCCCAGCTTACGCTCAAAAGCTCCTTGTCATGCTCGTTGAGTTTGATTACATCAAAATCTCCAACTTATTGCGTCACAATACGGTTTCACAATGCTTCGAGTTTCTTCTCGGAGACTTATCAAGCGCAAATGTGAACAATGTCAAGCTCTGTCTCGCCTTGGCTTCAGCTCCAGAGATGGAAAGTAAACTACTCTCTCAGCTCAAAGTAGTGAGGAGAATCGGAAATCTGCTGGAATTCGTGAATGCCAAAGATATGGAAGATTTCCTAGAACCCACTTTAAGTCTCTGCAGAGCTTTTCTGCTACGGTCACTTGGGAACAAGAAAGGTCTCAGTTCCAACTACACAAAAGAGCCAACGCTTTTATCAGAGGCATCTTTTACGTTCGAGGTTGATCCACAGGAATGCATAAGAGACATTGCAGATTTCGGTAGCAACATTGGCCTATTCTTGCACTTTGCAGGCTTAGACGATGACACAAGCATCGCAGTCGCAGATATTGCCTCTGAATGTGTTGTGTTGCTACTCAAAGCGGCTTCGAGAGAAGCCACGACGGGATTCTTAACCAATCTTCCTAAGATCACACCGATTCTGGACTCGTGGCGCAGGAGGAAGAGCACAGAGCTACACTTGTTGGTTCTGAAGAGAGTCTTGCATTGTTTGGGTTATGCTTGCAAGCAGTATCTATCGCAGGCGATGATTTTGTCGATATCGGGACACGATGTCTCAAAGATCAACGCAATTGTCTCTGAGATGAAGAACTCAGACGCCGCTGGTCTTAACAGCATCGCTTCACTCGTTGCCATGGAGTTGCAGAGATTACCTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1366

Amino Acids

152.28

Weight (kDa)

7.33

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 252 5.3e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 247 3.9e-42 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 66 - 149 2.9e-07 ABC1 atypical kinase-like domain
HEAT_ULK4 PF23606 917 - 1111 1.4e-67 Serine/threonine-protein kinase ULK4-like, HEAT repeats
ARM_RUK PF24970 1118 - 1366 4.4e-107 RUNKEL ARM-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1530
Acc36I ACCTGC 2 cut(s) 2086, 2700
Acc65I GGTACC 1 cut(s) 476
AccB1I GGYRCC 2 cut(s) 476, 968
AccB7I CCANNNNNTGG 1 cut(s) 656
AccII CGCG 1 cut(s) 115
AciI CCGC 4 cut(s) 115, 1667, 3803, 4041
AcsI RAATTY 5 cut(s) 772, 1729, 1918, 2626, 3506
AcuI CTGAAG 2 cut(s) 3420, 3932
AcyI GRCGYC 4 cut(s) 956, 1527, 2794, 4038
AdeI CACNNNGTG 1 cut(s) 1433
AfaI GTAC 7 cut(s) 11, 174, 478, 561, 884, 1759, 1854
AfiI CCNNNNNNNGG 7 cut(s) 464, 656, 965, 978, 1367, 2161, 3079
AflIII ACRYGT 2 cut(s) 210, 1413
AhdI GACNNNNNGTC 1 cut(s) 3992
AjnI CCWGG 3 cut(s) 655, 973, 2232
AjuI GAANNNNNNNTTGG 4 cut(s) 219, 251, 3212, 3244
AloI GAACNNNNNNTCC 2 cut(s) 2544, 2576
Alw21I GWGCWC 4 cut(s) 1264, 2830, 3176, 3890
Alw44I GTGCAC 2 cut(s) 1260, 3172
Ama87I CYCGRG 1 cut(s) 1745
AoxI GGCC 5 cut(s) 946, 1559, 1948, 2214, 3712
ApaLI GTGCAC 2 cut(s) 1260, 3172
ApeKI GCWGC 8 cut(s) 262, 1043, 1421, 1772, 1991, 2406, 2419, 3083
ApoI RAATTY 5 cut(s) 772, 1729, 1918, 2626, 3506
Asp700I GAANNNNTTC 2 cut(s) 2519, 2884
Asp718I GGTACC 1 cut(s) 476
AspLEI GCGC 4 cut(s) 2411, 3028, 3398, 3876
AspS9I GGNCC 4 cut(s) 1223, 1793, 2214, 2801
AsuC2I CCSGG 3 cut(s) 887, 2077, 2742
AsuHPI GGTGA 8 cut(s) 254, 1069, 1382, 1448, 2012, 2861, 3059, 3122
AsuII TTCGAA 2 cut(s) 341, 1614
AvaI CYCGRG 1 cut(s) 1745
AvaII GGWCC 3 cut(s) 1223, 1793, 2801
AxyI CCTNAGG 1 cut(s) 2921
BaeGI GKGCMC 4 cut(s) 550, 971, 1264, 3176
BamHI GGATCC 3 cut(s) 144, 519, 2306
BanI GGYRCC 2 cut(s) 476, 968
BanII GRGCYC 1 cut(s) 2830
BarI GAAGNNNNNNTAC 2 cut(s) 2979, 3011
BauI CACGAG 2 cut(s) 2492, 3868
BbsI GAAGAC 4 cut(s) 80, 589, 1309, 2797
Bbv12I GWGCWC 4 cut(s) 1264, 2830, 3176, 3890
BbvI GCAGC 8 cut(s) 274, 1030, 1408, 1784, 2003, 2418, 2431, 3070
BccI CCATC 7 cut(s) 379, 506, 2071, 2546, 2896, 3082, 3442
BciT130I CCWGG 3 cut(s) 657, 975, 2234
BciVI GTATCC 1 cut(s) 2746
BclI TGATCA 1 cut(s) 1734
BcnI CCSGG 3 cut(s) 887, 2077, 2742
BfaI CTAG 9 cut(s) 666, 1005, 1431, 1437, 1515, 1997, 2526, 3005, 3539
BfmI CTRYAG 4 cut(s) 326, 1123, 2194, 3559
BfuAI ACCTGC 2 cut(s) 2086, 2700
BfuI GTATCC 1 cut(s) 2746
BglII AGATCT 1 cut(s) 736
Bme1390I CCNGG 6 cut(s) 657, 887, 975, 2077, 2234, 2742
Bme18I GGWCC 3 cut(s) 1223, 1793, 2801
BmeRI GACNNNNNGTC 1 cut(s) 3992
BmeT110I CYCGRG 1 cut(s) 1745
BmgT120I GGNCC 4 cut(s) 1223, 1793, 2214, 2801
BmiI GGNNCC 8 cut(s) 146, 478, 495, 521, 970, 1224, 1243, 2308
BmrFI CCNGG 6 cut(s) 657, 887, 975, 2077, 2234, 2742
BmrI ACTGGG 1 cut(s) 2221
BmsI GCATC 9 cut(s) 1759, 2093, 2497, 2966, 3013, 3103, 3653, 3762, 4065
BmuI ACTGGG 1 cut(s) 2221
BpiI GAAGAC 4 cut(s) 80, 589, 1309, 2797
BplI GAGNNNNNCTC 1 cut(s) 4078
BpmI CTGGAG 3 cut(s) 480, 2212, 3426
Bpu14I TTCGAA 2 cut(s) 341, 1614
BpuEI CTTGAG 3 cut(s) 161, 975, 2726
BpuMI CCSGG 3 cut(s) 887, 2077, 2742
BsaBI GATNNNNATC 1 cut(s) 3056
BsaHI GRCGYC 4 cut(s) 956, 1527, 2794, 4038
BsaI GGTCTC 2 cut(s) 1123, 3605
BsaJI CCNNGG 8 cut(s) 462, 655, 973, 1365, 1459, 2797, 3429, 4074
Bsc4I CCNNNNNNNGG 7 cut(s) 464, 656, 965, 978, 1367, 2161, 3079
Bse1I ACTGG 1 cut(s) 2216
Bse21I CCTNAGG 1 cut(s) 2921
Bse3DI GCAATG 2 cut(s) 2644, 3687
Bse8I GATNNNNATC 1 cut(s) 3056
BseBI CCWGG 3 cut(s) 657, 975, 2234
BseDI CCNNGG 8 cut(s) 462, 655, 973, 1365, 1459, 2797, 3429, 4074
BseGI GGATG 3 cut(s) 163, 445, 1779
BseJI GATNNNNATC 1 cut(s) 3056
BseLI CCNNNNNNNGG 7 cut(s) 464, 656, 965, 978, 1367, 2161, 3079
BseMI GCAATG 2 cut(s) 2644, 3687
BseNI ACTGG 1 cut(s) 2216
BseRI GAGGAG 3 cut(s) 636, 2522, 3499
BseSI GKGCMC 4 cut(s) 550, 971, 1264, 3176
BseXI GCAGC 8 cut(s) 274, 1030, 1408, 1784, 2003, 2418, 2431, 3070
BseYI CCCAGC 5 cut(s) 612, 712, 1571, 1840, 3274
BsgI GTGCAG 2 cut(s) 1179, 3231
Bsh1236I CGCG 1 cut(s) 115
Bsh1285I CGRYCG 2 cut(s) 81, 2358
BshFI GGCC 5 cut(s) 948, 1561, 1950, 2216, 3714
BshNI GGYRCC 2 cut(s) 476, 968
BsiEI CGRYCG 2 cut(s) 81, 2358
BsiHKAI GWGCWC 4 cut(s) 1264, 2830, 3176, 3890
BsiHKCI CYCGRG 1 cut(s) 1745
BsiSI CCGG 3 cut(s) 886, 2077, 2741
BslFI GGGAC 2 cut(s) 1236, 3999
BslI CCNNNNNNNGG 7 cut(s) 464, 656, 965, 978, 1367, 2161, 3079
BsmBI CGTCTC 1 cut(s) 947
BsmFI GGGAC 2 cut(s) 1236, 3999
BsmI GAATGC 5 cut(s) 1600, 1888, 2346, 3520, 3680
BsnI GGCC 5 cut(s) 948, 1561, 1950, 2216, 3714
Bso31I GGTCTC 2 cut(s) 1123, 3605
BsoBI CYCGRG 1 cut(s) 1745
Bsp119I TTCGAA 2 cut(s) 341, 1614
Bsp1286I GDGCHC 6 cut(s) 550, 971, 1264, 2830, 3176, 3890
Bsp1407I TGTACA 1 cut(s) 1852
Bsp19I CCATGG 2 cut(s) 2797, 4074
BspACI CCGC 4 cut(s) 115, 1667, 3803, 4041
BspANI GGCC 5 cut(s) 948, 1561, 1950, 2216, 3714
BspFNI CGCG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 1492
BspLI GGNNCC 8 cut(s) 146, 478, 495, 521, 970, 1224, 1243, 2308
BspMAI CTGCAG 3 cut(s) 330, 2198, 3563
BspMI ACCTGC 2 cut(s) 2086, 2700
BspQI GCTCTTC 3 cut(s) 2583, 2890, 3878
BspT104I TTCGAA 2 cut(s) 341, 1614
BspT107I GGYRCC 2 cut(s) 476, 968
BspTNI GGTCTC 2 cut(s) 1123, 3605
BsrDI GCAATG 2 cut(s) 2644, 3687
BsrGI TGTACA 1 cut(s) 1852
BsrI ACTGG 1 cut(s) 2216
BssECI CCNNGG 8 cut(s) 462, 655, 973, 1365, 1459, 2797, 3429, 4074
BssNI GRCGYC 4 cut(s) 956, 1527, 2794, 4038
BssSI CACGAG 2 cut(s) 2492, 3868
BssT1I CCWWGG 4 cut(s) 1365, 2797, 3429, 4074
Bst2BI CACGAG 2 cut(s) 2492, 3868
Bst2UI CCWGG 3 cut(s) 657, 975, 2234
Bst6I CTCTTC 8 cut(s) 702, 755, 1459, 2583, 2890, 2965, 3878, 3908
BstACI GRCGYC 4 cut(s) 956, 1527, 2794, 4038
BstAPI GCANNNNNTGC 2 cut(s) 2253, 3770
BstAUI TGTACA 1 cut(s) 1852
BstBI TTCGAA 2 cut(s) 341, 1614
BstC8I GCNNGC 8 cut(s) 94, 798, 2329, 2747, 3733, 3943, 3947, 3963
BstDSI CCRYGG 3 cut(s) 462, 2797, 4074
BstENI CCTNNNNNAGG 1 cut(s) 2159
BstF5I GGATG 3 cut(s) 163, 445, 1779
BstFNI CGCG 1 cut(s) 115
BstHHI GCGC 4 cut(s) 2411, 3028, 3398, 3876
BstMCI CGRYCG 2 cut(s) 81, 2358
BstMWI GCNNNNNNNGC 8 cut(s) 406, 793, 1567, 1772, 2213, 2253, 3711, 3770
BstNI CCWGG 3 cut(s) 657, 975, 2234
BstNSI RCATGY 3 cut(s) 214, 1417, 2749
BstSCI CCNGG 6 cut(s) 655, 885, 973, 2075, 2232, 2740
BstSFI CTRYAG 4 cut(s) 326, 1123, 2194, 3559
BstSLI GKGCMC 4 cut(s) 550, 971, 1264, 3176
BstUI CGCG 1 cut(s) 115
BstV1I GCAGC 8 cut(s) 274, 1030, 1408, 1784, 2003, 2418, 2431, 3070
BstV2I GAAGAC 4 cut(s) 80, 589, 1309, 2797
BstX2I RGATCY 6 cut(s) 144, 519, 637, 736, 2306, 3265
BstXI CCANNNNNNTGG 1 cut(s) 3526
BstYI RGATCY 6 cut(s) 144, 519, 637, 736, 2306, 3265
Bsu36I CCTNAGG 1 cut(s) 2921
BsuI GTATCC 1 cut(s) 2746
BsuRI GGCC 5 cut(s) 948, 1561, 1950, 2216, 3714
BtgI CCRYGG 3 cut(s) 462, 2797, 4074
BtgZI GCGATG 3 cut(s) 3739, 3980, 4042
BtsCI GGATG 3 cut(s) 163, 445, 1779
BtsIMutI CAGTG 3 cut(s) 1179, 1521, 3248
BveI ACCTGC 2 cut(s) 2086, 2700
Cac8I GCNNGC 8 cut(s) 94, 798, 2329, 2747, 3733, 3943, 3947, 3963
CciI TCATGA 1 cut(s) 1492
CfoI GCGC 4 cut(s) 2411, 3028, 3398, 3876
Cfr13I GGNCC 4 cut(s) 1223, 1793, 2214, 2801
CseI GACGC 4 cut(s) 964, 2802, 3332, 4046
Csp6I GTAC 7 cut(s) 10, 173, 477, 560, 883, 1758, 1853
CspCI CAANNNNNGTGG 2 cut(s) 2931, 2966
CviQI GTAC 7 cut(s) 10, 173, 477, 560, 883, 1758, 1853
DraIII CACNNNGTG 1 cut(s) 1433
DriI GACNNNNNGTC 1 cut(s) 3992
Eam1104I CTCTTC 8 cut(s) 702, 755, 1459, 2583, 2890, 2965, 3878, 3908
Eam1105I GACNNNNNGTC 1 cut(s) 3992
EarI CTCTTC 8 cut(s) 702, 755, 1459, 2583, 2890, 2965, 3878, 3908
Ecl136II GAGCTC 1 cut(s) 2828
Eco130I CCWWGG 4 cut(s) 1365, 2797, 3429, 4074
Eco147I AGGCCT 1 cut(s) 1561
Eco24I GRGCYC 1 cut(s) 2830
Eco31I GGTCTC 2 cut(s) 1123, 3605
Eco32I GATATC 2 cut(s) 2770, 3981
Eco47I GGWCC 3 cut(s) 1223, 1793, 2801
Eco53kI GAGCTC 1 cut(s) 2828
Eco57I CTGAAG 2 cut(s) 3420, 3932
Eco81I CCTNAGG 1 cut(s) 2921
Eco88I CYCGRG 1 cut(s) 1745
EcoICRI GAGCTC 1 cut(s) 2828
EcoNI CCTNNNNNAGG 1 cut(s) 2159
EcoO109I RGGNCCY 1 cut(s) 1223
EcoRI GAATTC 1 cut(s) 3506
EcoRII CCWGG 3 cut(s) 655, 973, 2232
EcoRV GATATC 2 cut(s) 2770, 3981
EcoT14I CCWWGG 4 cut(s) 1365, 2797, 3429, 4074
EcoT22I ATGCAT 2 cut(s) 754, 3680
EcoT38I GRGCYC 1 cut(s) 2830
ErhI CCWWGG 4 cut(s) 1365, 2797, 3429, 4074
Esp3I CGTCTC 1 cut(s) 947
FalI AAGNNNNNCTT 8 cut(s) 574, 606, 984, 1016, 1456, 1488, 3905, 3937
FaqI GGGAC 2 cut(s) 1236, 3999
FbaI TGATCA 1 cut(s) 1734
FokI GGATG 3 cut(s) 150, 452, 1766
FriOI GRGCYC 1 cut(s) 2830
FspBI CTAG 9 cut(s) 666, 1005, 1431, 1437, 1515, 1997, 2526, 3005, 3539
GlaI GCGC 4 cut(s) 2410, 3027, 3397, 3875
GsaI CCCAGC 5 cut(s) 616, 716, 1575, 1844, 3278
GsuI CTGGAG 3 cut(s) 480, 2212, 3426
HaeIII GGCC 5 cut(s) 948, 1561, 1950, 2216, 3714
HapII CCGG 3 cut(s) 886, 2077, 2741
HgaI GACGC 4 cut(s) 964, 2802, 3332, 4046
HhaI GCGC 4 cut(s) 2411, 3028, 3398, 3876
Hin1I GRCGYC 4 cut(s) 956, 1527, 2794, 4038
Hin6I GCGC 4 cut(s) 2409, 3026, 3396, 3874
HinP1I GCGC 4 cut(s) 2409, 3026, 3396, 3874
HindIII AAGCTT 3 cut(s) 998, 2366, 2476
HpaII CCGG 3 cut(s) 886, 2077, 2741
HphI GGTGA 8 cut(s) 254, 1069, 1382, 1448, 2012, 2861, 3059, 3122
Hpy99I CGWCG 1 cut(s) 3826
HpyCH4IV ACGT 4 cut(s) 171, 472, 1527, 3653
HpyF10VI GCNNNNNNNGC 8 cut(s) 406, 793, 1567, 1772, 2213, 2253, 3711, 3770
HpySE526I ACGT 4 cut(s) 171, 472, 1527, 3653
Hsp92I GRCGYC 4 cut(s) 956, 1527, 2794, 4038
HspAI GCGC 4 cut(s) 2409, 3026, 3396, 3874
KpnI GGTACC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 1734
LguI GCTCTTC 3 cut(s) 2583, 2890, 3878
LmnI GCTCC 6 cut(s) 499, 1701, 2204, 2833, 3297, 3445
Lsp1109I GCAGC 8 cut(s) 274, 1030, 1408, 1784, 2003, 2418, 2431, 3070
LweI GCATC 9 cut(s) 1759, 2093, 2497, 2966, 3013, 3103, 3653, 3762, 4065
MaeI CTAG 9 cut(s) 666, 1005, 1431, 1437, 1515, 1997, 2526, 3005, 3539
MaeII ACGT 4 cut(s) 171, 472, 1527, 3653
MaeIII GTNAC 8 cut(s) 572, 2494, 2687, 2905, 2986, 3034, 3344, 3579
MfeI CAATTG 1 cut(s) 4011
MflI RGATCY 6 cut(s) 144, 519, 637, 736, 2306, 3265
MhlI GDGCHC 6 cut(s) 550, 971, 1264, 2830, 3176, 3890
MlyI GAGTC 6 cut(s) 701, 1490, 1928, 2152, 3860, 3927
MmeI TCCRAC 5 cut(s) 665, 2797, 3243, 3357, 3633
Mph1103I ATGCAT 2 cut(s) 754, 3680
MroXI GAANNNNTTC 2 cut(s) 2519, 2884
MslI CAYNNNNRTG 3 cut(s) 1184, 2450, 3673
MspA1I CMGCKG 3 cut(s) 1421, 1669, 4043
MspI CCGG 3 cut(s) 886, 2077, 2741
MspR9I CCNGG 6 cut(s) 657, 887, 975, 2077, 2234, 2742
MunI CAATTG 1 cut(s) 4011
Mva1269I GAATGC 5 cut(s) 1600, 1888, 2346, 3520, 3680
MvaI CCWGG 3 cut(s) 657, 975, 2234
MvnI CGCG 1 cut(s) 115
MwoI GCNNNNNNNGC 8 cut(s) 406, 793, 1567, 1772, 2213, 2253, 3711, 3770
NciI CCSGG 3 cut(s) 887, 2077, 2742
NcoI CCATGG 2 cut(s) 2797, 4074
NlaIV GGNNCC 8 cut(s) 146, 478, 495, 521, 970, 1224, 1243, 2308
NmuCI GTSAC 3 cut(s) 2494, 3344, 3579
NsiI ATGCAT 2 cut(s) 754, 3680
NspI RCATGY 3 cut(s) 214, 1417, 2749
NspV TTCGAA 2 cut(s) 341, 1614
PaeI GCATGC 1 cut(s) 2749
PaeR7I CTCGAG 1 cut(s) 1745
PagI TCATGA 1 cut(s) 1492
PceI AGGCCT 1 cut(s) 1561
PciI ACATGT 2 cut(s) 210, 1413
PciSI GCTCTTC 3 cut(s) 2583, 2890, 3878
PcsI WCGNNNNNNNCGW 1 cut(s) 4065
PctI GAATGC 5 cut(s) 1600, 1888, 2346, 3520, 3680
PdmI GAANNNNTTC 2 cut(s) 2519, 2884
PflMI CCANNNNNTGG 1 cut(s) 656
Ple19I CGATCG 1 cut(s) 81
PleI GAGTC 6 cut(s) 700, 1490, 1928, 2152, 3860, 3926
PpsI GAGTC 6 cut(s) 700, 1490, 1928, 2152, 3860, 3926
PpuMI RGGWCCY 1 cut(s) 1223
PscI ACATGT 2 cut(s) 210, 1413
Psp124BI GAGCTC 1 cut(s) 2830
Psp5II RGGWCCY 1 cut(s) 1223
Psp6I CCWGG 3 cut(s) 655, 973, 2232
PspFI CCCAGC 5 cut(s) 612, 712, 1571, 1840, 3274
PspGI CCWGG 3 cut(s) 655, 973, 2232
PspN4I GGNNCC 8 cut(s) 146, 478, 495, 521, 970, 1224, 1243, 2308
PspPI GGNCC 4 cut(s) 1223, 1793, 2214, 2801
PspPPI RGGWCCY 1 cut(s) 1223
PstI CTGCAG 3 cut(s) 330, 2198, 3563
PsuI RGATCY 6 cut(s) 144, 519, 637, 736, 2306, 3265
PvuI CGATCG 1 cut(s) 81
PvuII CAGCTG 1 cut(s) 1421
RsaI GTAC 7 cut(s) 11, 174, 478, 561, 884, 1759, 1854
RsaNI GTAC 7 cut(s) 10, 173, 477, 560, 883, 1758, 1853
RseI CAYNNNNRTG 3 cut(s) 1184, 2450, 3673
SacI GAGCTC 1 cut(s) 2830
SapI GCTCTTC 3 cut(s) 2583, 2890, 3878
Sau96I GGNCC 4 cut(s) 1223, 1793, 2214, 2801
SchI GAGTC 6 cut(s) 701, 1490, 1928, 2152, 3860, 3927
ScrFI CCNGG 6 cut(s) 657, 887, 975, 2077, 2234, 2742
SduI GDGCHC 6 cut(s) 550, 971, 1264, 2830, 3176, 3890
SfaNI GCATC 9 cut(s) 1759, 2093, 2497, 2966, 3013, 3103, 3653, 3762, 4065
SfcI CTRYAG 4 cut(s) 326, 1123, 2194, 3559
Sfr274I CTCGAG 1 cut(s) 1745
SfuI TTCGAA 2 cut(s) 341, 1614
SinI GGWCC 3 cut(s) 1223, 1793, 2801
SlaI CTCGAG 1 cut(s) 1745
SmiMI CAYNNNNRTG 3 cut(s) 1184, 2450, 3673
SmlI CTYRAG 4 cut(s) 176, 990, 1745, 2705
SmoI CTYRAG 4 cut(s) 176, 990, 1745, 2705
SphI GCATGC 1 cut(s) 2749
SseBI AGGCCT 1 cut(s) 1561
SsiI CCGC 4 cut(s) 115, 1667, 3803, 4041
SspI AATATT 2 cut(s) 230, 1063
SspMI CTAG 9 cut(s) 666, 1005, 1431, 1437, 1515, 1997, 2526, 3005, 3539
SstI GAGCTC 1 cut(s) 2830
StuI AGGCCT 1 cut(s) 1561
StyD4I CCNGG 6 cut(s) 655, 885, 973, 2075, 2232, 2740
StyI CCWWGG 4 cut(s) 1365, 2797, 3429, 4074
TaiI ACGT 4 cut(s) 174, 475, 1530, 3656
TatI WGTACW 2 cut(s) 559, 1852
TauI GCSGC 2 cut(s) 3806, 4043
TscAI CASTG 3 cut(s) 1186, 1528, 3255
TseFI GTSAC 3 cut(s) 2494, 3344, 3579
TseI GCWGC 8 cut(s) 262, 1043, 1421, 1772, 1991, 2406, 2419, 3083
Tsp45I GTSAC 3 cut(s) 2494, 3344, 3579
TspGWI ACGGA 5 cut(s) 1806, 2152, 2309, 2972, 3050
TspRI CASTG 3 cut(s) 1186, 1528, 3255
Van91I CCANNNNNTGG 1 cut(s) 656
VneI GTGCAC 2 cut(s) 1260, 3172
VpaK11BI GGWCC 3 cut(s) 1223, 1793, 2801
XagI CCTNNNNNAGG 1 cut(s) 2159
XapI RAATTY 5 cut(s) 772, 1729, 1918, 2626, 3506
XbaI TCTAGA 1 cut(s) 3004
XceI RCATGY 3 cut(s) 214, 1417, 2749
XhoI CTCGAG 1 cut(s) 1745
XmnI GAANNNNTTC 2 cut(s) 2519, 2884
XspI CTAG 9 cut(s) 666, 1005, 1431, 1437, 1515, 1997, 2526, 3005, 3539
ZraI GACGTC 1 cut(s) 1528
Zsp2I ATGCAT 2 cut(s) 754, 3680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.