FvH4_4g17050

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011033
Physical Location & Seq
Forward (+)
20954717 .. 20961227
6511 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17050.t1

Sequence Viewer

Length: 4107 bp
ATGAACCAGTACCACATATACGAGGCCATCGGCCGTGGAAAGTGCTCGACTGTGTACAAAGGGAGGAAGAAGAAGACGATTGAGTACTACGCGATTAAGAGCGTCGAGAAATCTCAGAAGAGCAAGCTTCTTCAGGAAGTTAAGATTCTTCATACTCTAGATCATCAGAATATTCTCAAGTTTTATTGGTGGTATGAAACTTCTGCTCACTTGTGGTTGGTTCTGGAGTATTGCGTTGGTGGGAATTTGATGACATTGTTGGACCAGGATCGTCAGCTACCTGAAGAGTCGATTCATGACCTTGGGTGGGATCTTGTTAGAGCTTTGCTGTTCTTACATTCAAAGGGTATCATATACTGTGATTTGAAACCATCAAATATCCTGTTGGACGAGAATGGCCGTACAAAGCTCTGTGATTTTGGACTGGCAAGAAAGCTGAGCGATATATCACAAACCTCTTCTTCCTCGTTGCCACAAGCAAAACGTGGAACACCTTGTTATATGGCTCCTGAGCTTTTTGAAGACGGGGGTGTCCATTCATATGCATCTGATTTCTGGGCCCTTGGTTGTGTACTATATGAGTGCTATGCTGGGAGGCCTCCATTTGTGGGTAGAGAATTTACTCAGCTAGTGAAGTCCATCCTCTCAGATCCAATACCTCCTCTACCTGGCACTCCTAGTCGTCCTTTTGTCAATCTAATAAATTCTTTACTCATCAAGGACCCAGCTGAACGAGTACAGTGGCCTGAACTCTGTGGTCATGCTTTCTGGAGAACCAAGTTAAATCCAGTGCCTCTACCTCCTCAGCCTGCTTTTGCGAATATGCTAGAGCAATATGCCAAGCCATTGTCAATACGAAATGGTGATAAATCTTCTGAGAATAGGACTCCTAAATCTCGTCAAAAGGATGTCAAGGGAGCTTCAAAGCAGGATGAGAATTCTATTGTAGGATCAAAAGGCCATGGAACTCCAGTCAAAGGCACACCTGGAAGTCGGAGAACTCAAGCAAAGGTATCTGGCAGAGGAGTTGATGAAAAGCAAAAGGATCATTCTGGCACTACCAGGGGCGTGAATCTCCTAAGACTCTCAAGAATAGCAAAGCAGAACTTGCAGAGGGAAAATGAGAAAGAGAACTATCGGAGGCCGGTGTCTAATAATTCTGAGAATGATTCAGAAGTCAAAATTGAGAACACTGATATGGAACTTGACTTTAATGAAAACACTGAAGATGATGCACAAGATGAATCTGATGGGCCAGATAACCAAGCTTCACTGGATGAAAAATCTTCAAGTCAAAATCAGCAGCAGGTTAAAGCAGAAGAGATGGAAAATAACATAAACCATTCAGATACCTCATCTGTGGTAAATGCGCCTGCATCAAATGAATCAACAATATGTGATCAGGAATCACCACAGAGTTTTGACGTGGCTGCAACTCCATGCAGTGCCAGTCCTCAGATAAAAAATCATAGATTTAAGGAAGGGTCTGGATCTGCAGTTGACTACGATTCTTCAAAATCTTCAAATAACCTTTCAGAGGTTCTTTGGCATCCTTCTGATCTTTCAGTCAGACCTGTAATGCCTAGCAGAAAAGCTGATAAAACTTCGGAGGTTATTCCTTCACTTCCCTTTGAGGCACTGCAAGCTGCTGATTTTGTGAAGATGTCTAAAGAGCAATTGGATACACTCACCAATAAGATTGTATCCATTTTTAATGGGAACATCAGCATTGGGGAGAAGCAGAATGCAATTAGATACCTTGAGATGTTGACCAACAATGCGGATGCTGCTAATATCTTGACCAATGGACCCATCATGCTTATCCTTGTTAAAATGCTTCGACAGACCAAGGCTTTGGCTCTGCGTGTCCAAGTTGCTTCACTGATTGGTTTGTTGATCCGACATTCTACTTTTATTCATGATGATTTGGCAAATTCTGGAATTTTGGGGTCACTGGCCGATGGCCTTGGAGACAGGCAGGAAAAAGTTAGGAGATTTTCTATGGCCGCTTTAGGTGAGCTGCTATTCTATATTTCAACTCAAAGTGAGCATGCTGGAGATAACAATCCTGTAGAGTCTCCATCCAAGGAATGCCGGTCCACATCTGGGTGGCAGGTCTCCAATTTGTTGCTTTCCAAAGTGGCATCAATTTTACGAAAAGGAGAGGATGACATAACTCAACTTTATGCATTGAGGACGATTGAAAATATATGTAGCCAAGCAGGGCACTGGGCAGCTCGTTTGAACAGCCAGGGCATGATTAATAACCTGTGCTATATATATAGGGCTACTGGGAAACTGGAAAGCATGAGACTCACAGCAGGATCATGTTTAGTCCGCCTGGTTCGTTTCAATCCACCTAGCATTCAGCAAGTAATTGATATTCTTCCTTTAAAGGAAATAGCATCTGCAATTGTCAAGGGCAGTCTGAGGGAGCAGCAGATCAGCTTAAACCTTTTGAACATGGCCATGCTTGGAAGTCACATGTTCACAAACTTTGGGAGGTACCTCTTGCCACTGATGGAGGATAAGAATATTGTTCCAAGTCTTGTGTCTCTTATTGAGCAGGGAAGTGAAGTTTTGAAAGGAAAAGCACTTGTTTTAGTTTCTCTTCTTTGTAAGAACAGCAGTTCCAAGAGTAGTAGGAGGTGGCTGCCACATTTTTTTTGCAATGCCAGGTTACTGTCCATGGTGGACAGGCTGGCAAAAGAGAAAGATCAGTATGTGAAACAGTGTTTAGATGCATTTGTGTGTGTTGTGGCCTCTGTTATACCTGGTTTACTGGATACTATAACCGGTGACTTTCAGCAAATGATAGGAAGGCGCCATGGACATCTGTCTCCCCTCAACTGTAGAGCTACTCCAAAGACCAACATTCATATGCTCCCTGTAGTTCTCCATCTTCTTCGAAGCTCATCTTTTAAGCATAAGGTGGTGAGTGATCAGGTCCTGCAGCAGTTGGCAAATCTAATAAAAATCGTGGAAACACCATTTCAGGGCAGGGATGACTTTCAAATGCATCTACTTCGAATTCTTGAATCAGTTGCTGAGGAGTCACCTGTAATTCTCAGAAGCCCTGATATTTTTACCCGTGAGGTCCTCCCCAGTTTGTCTGTTCTGTATAAGGGAAACAAGGATGGTGATGCCAGGTTTCTGTGCTTAAAAATCTTGTTTGATGTGATGGTAATTTTTCTTAATGAACAATCTGAAGATGAGCAAAGGTCCAAAGAATTGCAATCCATATCCAACACAAACTTTCTTCCTCTCTACCCTTCCTTGATTGAAGATGAAGATCCAATTCCCATGTATGCACAAAAGCTGCTAGTTATGCTGATTGATTATAATTTCATCAAAATCGCAGACATTCTTCATCTGAAGATAGTATCGCAATGCTTTGAGTTTTTGCTGGGTGATCTGTCAAGTGCAAATGTAAACAATGTTATGCTATGCCTGGCTCTGACATCTGCTCCTGAAATGGAAACCAAGTTACTTTCTCAGCTGAGGGTAGTTAGGAAAATAGGAAATCTTCTAGAGCTTGTATATGCAAAAGATATGGAAGATTTTCTGGAACCAACACTTGGTCTGTGTAGGGCTTTCCTTCTACGTTCAGTGAGTGGTAGAAAAGGCTTCACCTATTCATCAGAACCTACTCTTTTGGGTGATGTGTCTTTTGAGCCAAGTGGTGATCAGCAGCAAGGTATACGAGATATCATGGACTTTGGAAGCAATGTTGGTGTATTGCTAGAGTTAAGTAGTTCTCATGGAGGAAATGTGGCAGATATAGCTTCAGAATGTGTTGTGTTGTTGCTAAAGGCAGCTCCTAGGGAAGGCACCGCTGGTCTACTAACTAATCTTCCAAAAGTTACTGCAATGCTTGAGTCTTGGCGTAGAGGCACATCTCATTTGTTCGTGCAGCGAGTGCTACATGCACTTGGTTATGCTTGTAGGCAGTATTTGTTACATGCAATGATACTGTCGATATCATTACCTGAGATTCCAAGAATTGAGAGCATAGTATCTGAGCTTAAGGGTTCGGGTGTACCTGCTTTAGCCACTGCTGCTTTTCATGTGGCTGTGGAGTTGCAGCGGCTTCCTCGCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1369

Amino Acids

152.32

Weight (kDa)

6.9

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 256 2.3e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 251 1.5e-37 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 66 - 149 6.6e-07 ABC1 atypical kinase-like domain
HEAT_ULK4 PF23606 927 - 1119 7.9e-70 Serine/threonine-protein kinase ULK4-like, HEAT repeats
ARM_RUK PF24970 1126 - 1367 6.8e-118 RUNKEL ARM-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3342
AasI GACNNNNNNGTC 1 cut(s) 530
Acc36I ACCTGC 3 cut(s) 1297, 2104, 4051
Acc65I GGTACC 1 cut(s) 2505
AccB1I GGYRCC 3 cut(s) 2505, 2823, 3830
AccB7I CCANNNNNTGG 1 cut(s) 3578
AccI GTMKAC 2 cut(s) 3700, 3841
AccII CGCG 1 cut(s) 92
AciI CCGC 5 cut(s) 1781, 2007, 2338, 3834, 4087
AclWI GGATC 9 cut(s) 276, 318, 644, 958, 1053, 1498, 1891, 2332, 3287
AcoI YGGCCR 5 cut(s) 31, 397, 1956, 2004, 2466
AcsI RAATTY 7 cut(s) 244, 617, 703, 937, 1933, 1941, 3030
AcuI CTGAAG 6 cut(s) 116, 303, 1245, 3228, 3395, 3771
AcyI GRCGYC 1 cut(s) 2824
AfaI GTAC 8 cut(s) 11, 56, 86, 403, 573, 738, 2507, 4041
AfiI CCNNNNNNNGG 9 cut(s) 307, 608, 668, 977, 1537, 2106, 2996, 3578, 3827
AflII CTTAAG 1 cut(s) 4025
AflIII ACRYGT 1 cut(s) 2484
AgeI ACCGGT 1 cut(s) 2795
AjiI CACGTC 1 cut(s) 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 3561, 3593
AloI GAACNNNNNNTCC 2 cut(s) 2615, 2647
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 6 cut(s) 1965, 2082, 2122, 2305, 2559, 2844
AlwI GGATC 9 cut(s) 276, 318, 644, 958, 1053, 1498, 1891, 2332, 3287
AlwNI CAGNNNCTG 2 cut(s) 2950, 3047
ApaI GGGCCC 1 cut(s) 562
ApoI RAATTY 7 cut(s) 244, 617, 703, 937, 1933, 1941, 3030
ArsI GACNNNNNNTTYG 2 cut(s) 1837, 1869
AseI ATTAAT 1 cut(s) 2262
AsiGI ACCGGT 1 cut(s) 2795
Asp700I GAANNNNTTC 1 cut(s) 3561
Asp718I GGTACC 1 cut(s) 2505
AspA2I CCTAGG 1 cut(s) 3821
AspLEI GCGC 2 cut(s) 1372, 2826
AsuII TTCGAA 2 cut(s) 2908, 3028
AvaII GGWCC 7 cut(s) 262, 721, 1808, 2097, 2947, 3097, 3222
AvrII CCTAGG 1 cut(s) 3821
BaeGI GKGCMC 2 cut(s) 562, 2229
BalI TGGCCA 1 cut(s) 2468
BanI GGYRCC 3 cut(s) 2505, 2823, 3830
BanII GRGCYC 1 cut(s) 562
BbsI GAAGAC 2 cut(s) 80, 528
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 3 cut(s) 804, 3048, 3500
BceAI ACGGC 2 cut(s) 18, 384
BcgI CGANNNNNNTGC 2 cut(s) 3008, 3042
BciVI GTATCC 3 cut(s) 1675, 1714, 2779
BclI TGATCA 3 cut(s) 1399, 2941, 3685
BcoDI GTCTC 6 cut(s) 1965, 2082, 2122, 2305, 2559, 2844
BfmI CTRYAG 5 cut(s) 1494, 2070, 2851, 2889, 2951
BfoI RGCGCY 1 cut(s) 2827
BfrI CTTAAG 1 cut(s) 4025
BfuAI ACCTGC 3 cut(s) 1297, 2104, 4051
BfuI GTATCC 3 cut(s) 1675, 1714, 2779
BlnI CCTAGG 1 cut(s) 3821
BlpI GCTNAGC 1 cut(s) 437
BmcAI AGTACT 1 cut(s) 86
Bme18I GGWCC 7 cut(s) 262, 721, 1808, 2097, 2947, 3097, 3222
BmgBI CACGTC 1 cut(s) 1426
BmiI GGNNCC 8 cut(s) 507, 560, 723, 1810, 2507, 2825, 3570, 3832
BmrI ACTGGG 3 cut(s) 2239, 2301, 3099
BmuI ACTGGG 3 cut(s) 2239, 2301, 3099
BoxI GACNNNNGTC 1 cut(s) 2836
BpiI GAAGAC 2 cut(s) 80, 528
BpmI CTGGAG 4 cut(s) 245, 790, 954, 2076
Bpu10I CCTNAGC 4 cut(s) 510, 804, 3048, 3500
Bpu1102I GCTNAGC 1 cut(s) 437
Bpu14I TTCGAA 2 cut(s) 2908, 3028
BpuEI CTTGAG 5 cut(s) 161, 987, 1072, 1781, 3896
BsaBI GATNNNNATC 2 cut(s) 2064, 3291
BsaHI GRCGYC 1 cut(s) 2824
BsaI GGTCTC 1 cut(s) 2122
BsaWI WCCGGW 1 cut(s) 2795
BsaXI ACNNNNNCTCC 4 cut(s) 3451, 3481, 3756, 3786
Bsc4I CCNNNNNNNGG 9 cut(s) 307, 608, 668, 977, 1537, 2106, 2996, 3578, 3827
Bse118I RCCGGY 3 cut(s) 1144, 2094, 2795
Bse3DI GCAATG 5 cut(s) 2677, 3395, 3733, 3876, 3972
Bse8I GATNNNNATC 2 cut(s) 2064, 3291
BseJI GATNNNNATC 2 cut(s) 2064, 3291
BseLI CCNNNNNNNGG 9 cut(s) 307, 608, 668, 977, 1537, 2106, 2996, 3578, 3827
BseMI GCAATG 5 cut(s) 2677, 3395, 3733, 3876, 3972
BseRI GAGGAG 4 cut(s) 651, 792, 1038, 3065
BseSI GKGCMC 2 cut(s) 562, 2229
BseX3I CGGCCG 1 cut(s) 31
BseYI CCCAGC 3 cut(s) 590, 724, 3406
BsgI GTGCAG 1 cut(s) 3932
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 34
BshNI GGYRCC 3 cut(s) 2505, 2823, 3830
BshTI ACCGGT 1 cut(s) 2795
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 3 cut(s) 1145, 2095, 2796
BslI CCNNNNNNNGG 9 cut(s) 307, 608, 668, 977, 1537, 2106, 2996, 3578, 3827
BsmAI GTCTC 6 cut(s) 1965, 2082, 2122, 2305, 2559, 2844
BsmI GAATGC 3 cut(s) 1750, 2096, 2364
Bso31I GGTCTC 1 cut(s) 2122
Bsp119I TTCGAA 2 cut(s) 2908, 3028
Bsp120I GGGCCC 1 cut(s) 558
Bsp1286I GDGCHC 3 cut(s) 47, 562, 2229
Bsp1407I TGTACA 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 437
Bsp19I CCATGG 3 cut(s) 961, 2688, 2827
BspACI CCGC 5 cut(s) 1781, 2007, 2338, 3834, 4087
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 2 cut(s) 295, 1918
BspLI GGNNCC 8 cut(s) 507, 560, 723, 1810, 2507, 2825, 3570, 3832
BspMAI CTGCAG 2 cut(s) 1498, 2955
BspMI ACCTGC 3 cut(s) 1297, 2104, 4051
BspPI GGATC 9 cut(s) 276, 318, 644, 958, 1053, 1498, 1891, 2332, 3287
BspQI GCTCTTC 1 cut(s) 113
BspT104I TTCGAA 2 cut(s) 2908, 3028
BspT107I GGYRCC 3 cut(s) 2505, 2823, 3830
BspTI CTTAAG 1 cut(s) 4025
BspTNI GGTCTC 1 cut(s) 2122
BsrDI GCAATG 5 cut(s) 2677, 3395, 3733, 3876, 3972
BsrFI RCCGGY 3 cut(s) 1144, 2094, 2795
BsrGI TGTACA 1 cut(s) 54
BssAI RCCGGY 3 cut(s) 1144, 2094, 2795
BssNAI GTATAC 1 cut(s) 3701
BssNI GRCGYC 1 cut(s) 2824
BssT1I CCWWGG 9 cut(s) 301, 562, 961, 1848, 1966, 2085, 2688, 2827, 3821
Bst1107I GTATAC 1 cut(s) 3701
Bst4CI ACNGT 7 cut(s) 52, 359, 741, 2685, 2733, 2852, 3975
Bst6I CTCTTC 5 cut(s) 113, 279, 463, 1314, 2616
BstACI GRCGYC 1 cut(s) 2824
BstAFI CTTAAG 1 cut(s) 4025
BstAPI GCANNNNNTGC 2 cut(s) 1108, 3919
BstAUI TGTACA 1 cut(s) 54
BstBI TTCGAA 2 cut(s) 2908, 3028
BstC8I GCNNGC 6 cut(s) 125, 810, 1374, 1644, 2052, 2703
BstDSI CCRYGG 4 cut(s) 34, 961, 2688, 2827
BstENI CCTNNNNNAGG 2 cut(s) 1535, 3825
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 2827
BstHHI GCGC 2 cut(s) 1372, 2826
BstMAI GTCTC 6 cut(s) 1965, 2082, 2122, 2305, 2559, 2844
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 7 cut(s) 1108, 1643, 1787, 3328, 3782, 3919, 4058
BstNSI RCATGY 5 cut(s) 2054, 2488, 3929, 3965, 4105
BstPAI GACNNNNGTC 1 cut(s) 2836
BstSFI CTRYAG 5 cut(s) 1494, 2070, 2851, 2889, 2951
BstSLI GKGCMC 2 cut(s) 562, 2229
BstUI CGCG 1 cut(s) 92
BstV2I GAAGAC 2 cut(s) 80, 528
BstX2I RGATCY 4 cut(s) 310, 649, 1490, 3292
BstXI CCANNNNNNTGG 1 cut(s) 1855
BstYI RGATCY 4 cut(s) 310, 649, 1490, 3292
BstZ17I GTATAC 1 cut(s) 3701
BstZI CGGCCG 1 cut(s) 31
BsuI GTATCC 3 cut(s) 1675, 1714, 2779
BtgI CCRYGG 4 cut(s) 34, 961, 2688, 2827
BtrI CACGTC 1 cut(s) 1426
BtsI GCAGTG 3 cut(s) 1450, 1637, 4053
BveI ACCTGC 3 cut(s) 1297, 2104, 4051
Cac8I GCNNGC 6 cut(s) 125, 810, 1374, 1644, 2052, 2703
CaiI CAGNNNCTG 2 cut(s) 2950, 3047
CciI TCATGA 2 cut(s) 295, 1918
CfoI GCGC 2 cut(s) 1372, 2826
Cfr10I RCCGGY 3 cut(s) 1144, 2094, 2795
CseI GACGC 1 cut(s) 91
CsiI ACCWGGT 1 cut(s) 2773
Csp6I GTAC 8 cut(s) 10, 55, 85, 402, 572, 737, 2506, 4040
CspAI ACCGGT 1 cut(s) 2795
CviQI GTAC 8 cut(s) 10, 55, 85, 402, 572, 737, 2506, 4040
DinI GGCGCC 1 cut(s) 2825
DraI TTTAAA 1 cut(s) 2394
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 5 cut(s) 31, 397, 1956, 2004, 2466
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 5 cut(s) 113, 279, 463, 1314, 2616
EarI CTCTTC 5 cut(s) 113, 279, 463, 1314, 2616
EciI GGCGGA 1 cut(s) 2327
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 9 cut(s) 301, 562, 961, 1848, 1966, 2085, 2688, 2827, 3821
Eco147I AGGCCT 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 562
Eco31I GGTCTC 1 cut(s) 2122
Eco32I GATATC 2 cut(s) 3709, 3981
Eco47I GGWCC 7 cut(s) 262, 721, 1808, 2097, 2947, 3097, 3222
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 6 cut(s) 116, 303, 1245, 3228, 3395, 3771
EcoNI CCTNNNNNAGG 2 cut(s) 1535, 3825
EcoO109I RGGNCCY 4 cut(s) 559, 721, 2947, 3097
EcoRI GAATTC 2 cut(s) 937, 3030
EcoRV GATATC 2 cut(s) 3709, 3981
EcoT14I CCWWGG 9 cut(s) 301, 562, 961, 1848, 1966, 2085, 2688, 2827, 3821
EcoT22I ATGCAT 4 cut(s) 547, 2191, 2746, 3021
EcoT38I GRGCYC 1 cut(s) 562
EgeI GGCGCC 1 cut(s) 2825
EheI GGCGCC 1 cut(s) 2825
ErhI CCWWGG 9 cut(s) 301, 562, 961, 1848, 1966, 2085, 2688, 2827, 3821
FalI AAGNNNNNCTT 4 cut(s) 1091, 1123, 2902, 2934
FauNDI CATATG 2 cut(s) 541, 2880
FbaI TGATCA 3 cut(s) 1399, 2941, 3685
FblI GTMKAC 2 cut(s) 3700, 3841
FriOI GRGCYC 1 cut(s) 562
GlaI GCGC 2 cut(s) 1371, 2825
GsaI CCCAGC 3 cut(s) 594, 728, 3410
GsuI CTGGAG 4 cut(s) 245, 790, 954, 2076
HaeII RGCGCY 1 cut(s) 2827
HapII CCGG 3 cut(s) 1145, 2095, 2796
HgaI GACGC 1 cut(s) 91
HhaI GCGC 2 cut(s) 1372, 2826
Hin1I GRCGYC 1 cut(s) 2824
Hin6I GCGC 2 cut(s) 1370, 2824
HinP1I GCGC 2 cut(s) 1370, 2824
HincII GTYRAC 2 cut(s) 1501, 1770
HindII GTYRAC 2 cut(s) 1501, 1770
HindIII AAGCTT 2 cut(s) 125, 1266
HpaII CCGG 3 cut(s) 1145, 2095, 2796
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 7 cut(s) 1475, 1563, 1629, 2814, 3282, 3608, 3821
HpyCH4III ACNGT 7 cut(s) 52, 359, 741, 2685, 2733, 2852, 3975
HpyCH4IV ACGT 3 cut(s) 484, 1425, 3604
HpyF10VI GCNNNNNNNGC 7 cut(s) 1108, 1643, 1787, 3328, 3782, 3919, 4058
HpySE526I ACGT 3 cut(s) 484, 1425, 3604
Hsp92I GRCGYC 1 cut(s) 2824
HspAI GCGC 2 cut(s) 1370, 2824
KasI GGCGCC 1 cut(s) 2823
KpnI GGTACC 1 cut(s) 2509
Ksp22I TGATCA 3 cut(s) 1399, 2941, 3685
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 6 cut(s) 511, 917, 2434, 2889, 3472, 3823
MabI ACCWGGT 1 cut(s) 2773
MaeII ACGT 3 cut(s) 484, 1425, 3604
MaeIII GTNAC 8 cut(s) 1950, 2480, 2679, 2798, 3054, 3486, 3862, 3957
MfeI CAATTG 2 cut(s) 1676, 2412
MflI RGATCY 4 cut(s) 310, 649, 1490, 3292
MhlI GDGCHC 3 cut(s) 47, 562, 2229
MlsI TGGCCA 1 cut(s) 2468
MluNI TGGCCA 1 cut(s) 2468
Mly113I GGCGCC 1 cut(s) 2824
MlyI GAGTC 7 cut(s) 296, 880, 1077, 2084, 2307, 3062, 3887
MmeI TCCRAC 5 cut(s) 240, 366, 974, 1924, 3270
Mox20I TGGCCA 1 cut(s) 2468
Mph1103I ATGCAT 4 cut(s) 547, 2191, 2746, 3021
MroXI GAANNNNTTC 1 cut(s) 3561
MscI TGGCCA 1 cut(s) 2468
MslI CAYNNNNRTG 6 cut(s) 540, 1196, 2107, 2468, 2749, 2879
Msp20I TGGCCA 1 cut(s) 2468
MspA1I CMGCKG 4 cut(s) 728, 3499, 3836, 4087
MspCI CTTAAG 1 cut(s) 4025
MspI CCGG 3 cut(s) 1145, 2095, 2796
MunI CAATTG 2 cut(s) 1676, 2412
Mva1269I GAATGC 3 cut(s) 1750, 2096, 2364
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 7 cut(s) 1108, 1643, 1787, 3328, 3782, 3919, 4058
NarI GGCGCC 1 cut(s) 2824
NcoI CCATGG 3 cut(s) 961, 2688, 2827
NdeI CATATG 2 cut(s) 541, 2880
NlaIV GGNNCC 8 cut(s) 507, 560, 723, 1810, 2507, 2825, 3570, 3832
NmuCI GTSAC 4 cut(s) 1950, 2480, 2798, 3054
NsiI ATGCAT 4 cut(s) 547, 2191, 2746, 3021
NspI RCATGY 5 cut(s) 2054, 2488, 3929, 3965, 4105
NspV TTCGAA 2 cut(s) 2908, 3028
PaeI GCATGC 1 cut(s) 2054
PagI TCATGA 2 cut(s) 295, 1918
PceI AGGCCT 1 cut(s) 598
PciI ACATGT 1 cut(s) 2484
PciSI GCTCTTC 1 cut(s) 113
PctI GAATGC 3 cut(s) 1750, 2096, 2364
PdmI GAANNNNTTC 1 cut(s) 3561
PflMI CCANNNNNTGG 1 cut(s) 3578
PinAI ACCGGT 1 cut(s) 2795
PleI GAGTC 7 cut(s) 295, 880, 1077, 2083, 2307, 3061, 3886
PluTI GGCGCC 1 cut(s) 2827
PpsI GAGTC 7 cut(s) 295, 880, 1077, 2083, 2307, 3061, 3886
PpuMI RGGWCCY 3 cut(s) 721, 2947, 3097
PscI ACATGT 1 cut(s) 2484
PshAI GACNNNNGTC 1 cut(s) 2836
PshBI ATTAAT 1 cut(s) 2262
PsiI TTATAA 1 cut(s) 3342
Psp5II RGGWCCY 3 cut(s) 721, 2947, 3097
PspFI CCCAGC 3 cut(s) 590, 724, 3406
PspN4I GGNNCC 8 cut(s) 507, 560, 723, 1810, 2507, 2825, 3570, 3832
PspOMI GGGCCC 1 cut(s) 558
PspPPI RGGWCCY 3 cut(s) 721, 2947, 3097
PstI CTGCAG 2 cut(s) 1498, 2955
PstNI CAGNNNCTG 2 cut(s) 2950, 3047
PsuI RGATCY 4 cut(s) 310, 649, 1490, 3292
PvuII CAGCTG 2 cut(s) 728, 3499
RsaI GTAC 8 cut(s) 11, 56, 86, 403, 573, 738, 2507, 4041
RsaNI GTAC 8 cut(s) 10, 55, 85, 402, 572, 737, 2506, 4040
RseI CAYNNNNRTG 6 cut(s) 540, 1196, 2107, 2468, 2749, 2879
SapI GCTCTTC 1 cut(s) 113
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 7 cut(s) 296, 880, 1077, 2084, 2307, 3062, 3887
SduI GDGCHC 3 cut(s) 47, 562, 2229
SexAI ACCWGGT 1 cut(s) 2773
SfcI CTRYAG 5 cut(s) 1494, 2070, 2851, 2889, 2951
SfoI GGCGCC 1 cut(s) 2825
SfuI TTCGAA 2 cut(s) 2908, 3028
SinI GGWCC 7 cut(s) 262, 721, 1808, 2097, 2947, 3097, 3222
SmiMI CAYNNNNRTG 6 cut(s) 540, 1196, 2107, 2468, 2749, 2879
SmlI CTYRAG 6 cut(s) 176, 1002, 1087, 1760, 3875, 4025
SmoI CTYRAG 6 cut(s) 176, 1002, 1087, 1760, 3875, 4025
SphI GCATGC 1 cut(s) 2054
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 5 cut(s) 1781, 2007, 2338, 3834, 4087
SspDI GGCGCC 1 cut(s) 2823
SspI AATATT 2 cut(s) 172, 2536
StuI AGGCCT 1 cut(s) 598
StyI CCWWGG 9 cut(s) 301, 562, 961, 1848, 1966, 2085, 2688, 2827, 3821
TaaI ACNGT 7 cut(s) 52, 359, 741, 2685, 2733, 2852, 3975
TaiI ACGT 3 cut(s) 487, 1428, 3607
TaqI TCGA 7 cut(s) 47, 105, 290, 1840, 2908, 3028, 3977
TatI WGTACW 4 cut(s) 54, 84, 571, 736
TauI GCSGC 2 cut(s) 2009, 4090
TseFI GTSAC 4 cut(s) 1950, 2480, 2798, 3054
Tsp45I GTSAC 4 cut(s) 1950, 2480, 2798, 3054
Van91I CCANNNNNTGG 1 cut(s) 3578
Vha464I CTTAAG 1 cut(s) 4025
VpaK11BI GGWCC 7 cut(s) 262, 721, 1808, 2097, 2947, 3097, 3222
VspI ATTAAT 1 cut(s) 2262
XagI CCTNNNNNAGG 2 cut(s) 1535, 3825
XapI RAATTY 7 cut(s) 244, 617, 703, 937, 1933, 1941, 3030
XbaI TCTAGA 2 cut(s) 157, 3529
XceI RCATGY 5 cut(s) 2054, 2488, 3929, 3965, 4105
XmaJI CCTAGG 1 cut(s) 3821
XmiI GTMKAC 2 cut(s) 3700, 3841
XmnI GAANNNNTTC 1 cut(s) 3561
ZrmI AGTACT 1 cut(s) 86
Zsp2I ATGCAT 4 cut(s) 547, 2191, 2746, 3021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.