MD13G1174600.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011033
Physical Location & Seq
Reverse (-)
14380345 .. 14386662
6318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1174600.v1.1.491

Sequence Viewer

Length: 4023 bp
ATGAACCAGTACCACATCTACGAAGCCATTGGCCACGGAAAATGCTCGACTGTGTACAAAGGGAGGAAGAAGAAGACGATTGAGTACTTCGCGATTAAGAGCGTCGAGAAGTCACAGAAGAGCAAGCTTCTCCAAGAAGTGAAGATTCTTCACACTCTAGATCATCAAAATATCCTCAAGTTTTACTGGTGGTATGAAACTTCTGCTCACTTGTGGTTGGTTTTGGAGTATTGCGTTGGGGGCAATTTGATGACACTATTAAACCAGGATAAGCAGCTACCTGAAGAGTCAATTCATGACCTTGGGTGTGACCTTGTTAGAGCTTTGCTGTTTTTACACTCAAAGGGAATCATATACTGTGACTTGAAACCATCAAATATCTTGTTAGACGAGAATGGCCGTACAAAGCTCTGTGATTTTGGATTGGCGAGAAAATTGAGTGATATATCACAAATACCTTCTTCTTCATTGCCACAAGCGAAACGCGGGACACCTTGTTATATGGCTCCTGAGCTTTTTGAAGAAGGGGGTGTCCATTCATATGCATCTGATTTCTGGGCCCTTGGGTGTGTACTGTATGAGTGCTATGCTGGGAGGCCTCCTTTTGTGGGTAGGGAATTTACTCAGCTAGTAAAATCCATTATCTCAGATCCAACTCCGGCTCTACCTGGAACTCCTAGCCGTTCTTTTGTCAATCTAATAAATTTTTTACTCATAAAGGATCCAGCAGAAAGAATACAGTGGCCTGAACTTTGTGGTCATGCTTTCTGGAGGACTAAGTTAACTTTAGTGCCTCTACCTCCTCAGCCTGCTTTTGATAATATGCTTGAGCAATATGCTCGGCCGTGTCTGTCAGAGCGCAATGGTGATAAATCTTCCCAAATCAGAACTCCTAAATCTTGCCAAAAGGATGTTAAAGGAACTTTTAAGCAGGATGAGAATACTATTTTAGGAGCAAACGGTCATGAGACTCCAGTCAAGGGTACACCTGGAAGCCGCAGAACTCAAGCAAAGGTTTCTGGCAGAGGGGTTGGTGAAAAGCAAAAGGATCCTTCTGGTGCCACGAGGGGTGTGAATCTTTTGAGGCTCTCAAGAATTGCAAAACAGAACCTGCAGAGGGAAAATGAGAAGGAAAACTACCGCAGGCCAGTGTCTACTAATACTGAGAATGATTCTGAAGTCAAAATTGAGAACACGGACATGGAACTTGACTTTAATGAGAACACGGAAGATGACACACATGATGAATCTGATGGGTCTGATATCCAAGCTTGTCCACCCGACAACAAATTTTCAAGTCAGCACCAGGGTAAAATGGAAGAGATGGAAAACAACTTGAACCAATCAGATGCCCTGCCTGTGGTTAGTACACCTGCCTCAGATGAATCCGGAGTTCATGATCAGGAACCATCTCCCCAGAGTATTGAAATGGCTGCAATTCCACCCAGTGCTAGTCCTCAAATTAAAAATCAGAGATTTAAAGAAGGTTCAGGATCTGCAGTTGACTATGATTCTTCAAAATCTCCAAATAACCTTTCACAAGTTCTTTGGCATCCATCAGATCTCTCAGTCAGACCTGTGATGCCCAGCAGAAAATCTGACAAAAATTCGGAGGTAATTCCTTCTCTTCCCTTTGAGGCACTGCAAGCCTCTGACTTTGTGAAGATCTCTAAAGAGCAGTTGGATGCACTCAACAGTAAGATTATAGCCATTTTTAATGGGAACAGTAGCATTGGCGAGAAGCAGAATGTAATTAGATACCTTGAGATGTTAAGCAATAATGCTGATGCTGCAAATATCTTGACCAATGGACCTATCATGCTTCTGCTTGTTAAAATGCTCCGGTTGTCCAAGGCTTTGGCTTTGCGTGTCCAACTTGCTTCACTGGTTGGTTTGTTGATTAGACATTCTACTTTTATTCAAGATGATTTGGCAGATTCAGGAATTTTGGGTTCACTTGCTGATGGCCTCAGAGACAAGCAGGAAAAGGTGAGGAGGTTTTCAATGGCTGCCTTGGGTGAGTTGCTATTCTATATATCCACTCAAAGTGAGCAGGCAGACAGCAATCCAGCAGAGTCTCCGTCAAAGGAAAGCCGGTCCACATCTGGGTGGCAGGTTTCAAATGCATTGCTTTCATTGGTGTCATCAATTTTACGAAAAGGAGAGGATGACTTAACTCAGTTATATGCATTGAGGACAATAGAAAATATTTGCAGTCAAGCAGGGCCGTGGGCAGCTCGTTTGACTAGCCAGGACATGATTAATAACCTGTGTTATTTATATAGGGCTGCTGGAAAGCAGGAGAGCATGAGGCTCACAGCAGGATCATGCTTGGTCCGTCTGGTTCGTTTTAACCCACCTAGCATTCAACCAGTAATAGAGAAGCTCTCCTTAAAGGACATAGCATCGGCACTTGTGAAGGGCAGCCTGCGTGAGCAGCAAATAAGCTTAAACCTCTTAAACATGGCCATGCTTGGAAGTCATGTGTTCACGAATGTTGGCAGGTACCTCCTCCCATTGATGGAGGATAAGAATCTTGTCCCAAGTCTTGTGTCTCTTATTGATCAGGGAAGTGAGGTTTTGAAGGGAAAGGCACTTGTTTTTGTTGCTCTTCTTTGTAAGAGCAGTAGGAGGTGGCTGCCACATTTTTTTTGCCACGCCAAGTTGCTTTCTGCAGTGGACAGGTTGGTGAAAGAGAAGGACCGTTATGTGCAGCAGTGTTTAGAGGCATCTGTGTGTGTTGTGGCGTCTGCTATACCCAGTTTACTGGATACTATTACTGGAGATATTCAGAAAATGATGGGAGGTAGGCGCCATGGGTATCAATCTCCCCTTAACAGTAGAGCTGCTCCAAAGACCAATGTTTATACGTTTCCCGTAGTTCTCCATCTTCTTCGAAGCTTGTCATTTAAGCGCAAAGTGGTTAATGAACAGGTCCTGCAGCAGTTGGCAAATCTAATGAAACTCGTGGAGACACCATTTCAGGGCAGGGATGACTTTCAGATTACCCTTCTCCGAATTCTTGAGTCTGTCTCAGAGGAGTCGCATGTGATTCTTGAAAGCCCTGACATTTTTATTCGTGAGGTTCTCCCCAGTTTGGCTGTTCTTTATAAGGGTAACAAGGATGGTGATGCCAGGTTTCTGTGCTTGAAAATTTTGTTTGACGTGATGGTAATTTTTTTGAATGAACAATCTGAAGATCAACAAAGGTCCAAAGAACTGGAATCCATATCCAATAAGAATTTTCTTCCTCTCTACCCTTCCTTGATTGAAGATGAAGATCCGATTCCCTTGTACGCACAGAAGCTTCTTGTGATGCTTATTGAATTTAATTACATCAAAATTGCAGACATTCTAAATCTTAAGATAGTCTCACAATGCTTTGAGTTTTTGCTCGGCGATCTTTCAAGTGCAAATGTGAACAATGTCATGCTATGTCTGGCTCTGACATCGGCACCTGAGATGGAAACCAAGTTACTTTCTCAACTGAAGGTAGTTAGGAAAATAGGAAACCTTCTAGAGTTTGTATATGCAAAGGATATGGAAGATTTTCTGGAACCAACCCTTGGCCTATGTAGGGCTTTCCTTGTACGGTCAGTGAGTGGTAGAAAAGGCTTCATCTATTCAAAAGAACCTGATCTTCTGGGTGATGCGTCTTCTGAGGCAAGTGGTGAATGCGTTGTCTTGTTGCTCAAGGCAGCTCCAAGGGAAGCCACTGCTGGTCTGCTAACTAATCTTCCAAAAGTTACTGCAGTCCTCGAGTCTTGGCGTAGGGGCGCATCTCACTTATTGGTGCTGCGCGTGCTGCATGCCCTTGGTTATTCCTGTAGGCAATATTTGTTACACGCAATGATACTGTCGATATCTATACCCGAGATCTCAAGAATTGAAGGCATAGTTTCTGAGATTAAAAGTTCAGGTGTACATGTTTTAGCCACTGCTGCTTTCCATGTGGCTGTGGAGTTGCAACGGCTGCCTCGCTGCCTTGCTATGCAAATCAATCGGCTTCTCCTGAGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1341

Amino Acids

149.24

Weight (kDa)

7.11

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 256 1.1e-62 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 251 5.5e-37 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 66 - 148 2.8e-07 ABC1 atypical kinase-like domain
HEAT_ULK4 PF23606 922 - 1115 6.1e-75 Serine/threonine-protein kinase ULK4-like, HEAT repeats
ARM_RUK PF24970 1122 - 1223 2.4e-53 RUNKEL ARM-repeat domain
ARM_RUK PF24970 1221 - 1328 4.4e-43 RUNKEL ARM-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3111
AarI CACCTGC 1 cut(s) 1381
Acc36I ACCTGC 4 cut(s) 1119, 1381, 2104, 2493
Acc65I GGTACC 1 cut(s) 2505
AccB1I GGYRCC 4 cut(s) 1058, 2505, 2811, 3454
AccB7I CCANNNNNTGG 1 cut(s) 3566
AccI GTMKAC 1 cut(s) 1154
AccII CGCG 3 cut(s) 92, 486, 3801
AccIII TCCGGA 1 cut(s) 1388
AciI CCGC 3 cut(s) 486, 997, 1141
AclWI GGATC 8 cut(s) 644, 716, 729, 1043, 1056, 1501, 2332, 3275
AcoI YGGCCR 4 cut(s) 31, 397, 842, 2466
AcsI RAATTY 9 cut(s) 617, 703, 1289, 1606, 1944, 3018, 3153, 3241, 3326
AcuI CTGAAG 4 cut(s) 303, 1197, 3216, 3509
AcyI GRCGYC 2 cut(s) 2747, 2812
AdeI CACNNNGTG 1 cut(s) 1448
AfeI AGCGCT 1 cut(s) 4018
AflII CTTAAG 1 cut(s) 3362
AflIII ACRYGT 1 cut(s) 3925
AjiI CACGTC 1 cut(s) 3166
AjnI CCWGG 6 cut(s) 264, 667, 988, 1305, 2250, 3134
AjuI GAANNNNNNNTTGG 4 cut(s) 1519, 1551, 3549, 3581
Alw26I GTCTC 7 cut(s) 962, 1968, 2082, 2559, 2966, 3037, 3376
AlwI GGATC 8 cut(s) 644, 716, 729, 1043, 1056, 1501, 2332, 3275
AlwNI CAGNNNCTG 3 cut(s) 1111, 1496, 2938
Ama87I CYCGRG 2 cut(s) 3758, 3872
Aor13HI TCCGGA 1 cut(s) 1388
Aor51HI AGCGCT 1 cut(s) 4018
ApaI GGGCCC 1 cut(s) 562
ApoI RAATTY 9 cut(s) 617, 703, 1289, 1606, 1944, 3018, 3153, 3241, 3326
AseI ATTAAT 1 cut(s) 2262
Asp700I GAANNNNTTC 1 cut(s) 3549
Asp718I GGTACC 1 cut(s) 2505
AspLEI GCGC 6 cut(s) 861, 2814, 2916, 3779, 3801, 4019
AspS9I GGNCC 9 cut(s) 558, 559, 1811, 2097, 2225, 2335, 2701, 2935, 3210
AsuHPI GGTGA 8 cut(s) 878, 1046, 2002, 2030, 2701, 3140, 3659, 3683
AsuII TTCGAA 1 cut(s) 2896
AvaI CYCGRG 2 cut(s) 3758, 3872
AvaII GGWCC 6 cut(s) 1811, 2097, 2335, 2701, 2935, 3210
BaeGI GKGCMC 1 cut(s) 562
BalI TGGCCA 2 cut(s) 33, 2468
BamHI GGATCC 2 cut(s) 721, 1048
BanI GGYRCC 4 cut(s) 1058, 2505, 2811, 3454
BanII GRGCYC 1 cut(s) 562
BarI GAAGNNNNNNTAC 2 cut(s) 1121, 1153
BauI CACGAG 2 cut(s) 1063, 2966
BbsI GAAGAC 2 cut(s) 80, 3648
BbvCI CCTCAGC 1 cut(s) 804
BceAI ACGGC 5 cut(s) 384, 666, 829, 2212, 3986
BcgI CGANNNNNNTGC 4 cut(s) 821, 855, 3983, 4017
BciT130I CCWGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
BciVI GTATCC 1 cut(s) 2764
BclI TGATCA 2 cut(s) 1399, 2563
BcoDI GTCTC 7 cut(s) 962, 1968, 2082, 2559, 2966, 3037, 3376
BfaI CTAG 7 cut(s) 158, 629, 678, 1452, 2247, 2361, 3518
BfmI CTRYAG 6 cut(s) 1112, 1497, 2673, 2939, 3750, 3826
BfoI RGCGCY 2 cut(s) 2815, 4020
BfrI CTTAAG 1 cut(s) 3362
BfuAI ACCTGC 4 cut(s) 1119, 1381, 2104, 2493
BfuI GTATCC 1 cut(s) 2764
BglII AGATCT 3 cut(s) 1561, 1665, 3876
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
Bme18I GGWCC 6 cut(s) 1811, 2097, 2335, 2701, 2935, 3210
BmeT110I CYCGRG 2 cut(s) 3758, 3872
BmgBI CACGTC 1 cut(s) 3166
BmgT120I GGNCC 9 cut(s) 558, 559, 1811, 2097, 2225, 2335, 2701, 2935, 3210
BmrFI CCNGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
BmrI ACTGGG 3 cut(s) 1440, 2754, 3087
BmuI ACTGGG 3 cut(s) 1440, 2754, 3087
BoxI GACNNNNGTC 1 cut(s) 974
BpiI GAAGAC 2 cut(s) 80, 3648
BplI GAGNNNNNCTC 4 cut(s) 2372, 2404, 3017, 3049
BpmI CTGGAG 3 cut(s) 790, 957, 2802
Bpu10I CCTNAGC 3 cut(s) 510, 804, 4013
Bpu14I TTCGAA 1 cut(s) 2896
BpuEI CTTGAG 8 cut(s) 161, 848, 990, 1075, 1784, 3044, 3677, 3865
BsaBI GATNNNNATC 2 cut(s) 2532, 3279
BsaHI GRCGYC 2 cut(s) 2747, 2812
BsaWI WCCGGW 2 cut(s) 1388, 1842
Bse118I RCCGGY 1 cut(s) 2094
Bse3DI GCAATG 4 cut(s) 467, 868, 2126, 3855
Bse8I GATNNNNATC 2 cut(s) 2532, 3279
BseAI TCCGGA 1 cut(s) 1388
BseBI CCWGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
BseGI GGATG 7 cut(s) 916, 940, 1552, 1690, 2173, 2998, 3130
BseJI GATNNNNATC 2 cut(s) 2532, 3279
BseMI GCAATG 4 cut(s) 467, 868, 2126, 3855
BseRI GAGGAG 4 cut(s) 792, 2008, 2501, 3053
BseSI GKGCMC 1 cut(s) 562
BseX3I CGGCCG 1 cut(s) 842
BseYI CCCAGC 2 cut(s) 590, 1586
BsgI GTGCAG 1 cut(s) 2732
Bsh1236I CGCG 3 cut(s) 92, 486, 3801
Bsh1285I CGRYCG 1 cut(s) 845
BshNI GGYRCC 4 cut(s) 1058, 2505, 2811, 3454
BsiEI CGRYCG 1 cut(s) 845
BsiHKCI CYCGRG 2 cut(s) 3758, 3872
BsiSI CCGG 4 cut(s) 659, 1389, 1843, 2095
BslFI GGGAC 2 cut(s) 502, 2525
BsmAI GTCTC 7 cut(s) 962, 1968, 2082, 2559, 2966, 3037, 3376
BsmFI GGGAC 2 cut(s) 502, 2525
BsmI GAATGC 2 cut(s) 2364, 3680
BsoBI CYCGRG 2 cut(s) 3758, 3872
Bsp119I TTCGAA 1 cut(s) 2896
Bsp120I GGGCCC 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 562
Bsp13I TCCGGA 1 cut(s) 1388
Bsp1407I TGTACA 2 cut(s) 54, 3922
Bsp19I CCATGG 1 cut(s) 2815
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 3 cut(s) 486, 997, 1141
BspEI TCCGGA 1 cut(s) 1388
BspFNI CGCG 3 cut(s) 92, 486, 3801
BspHI TCATGA 3 cut(s) 295, 964, 1396
BspMAI CTGCAG 5 cut(s) 1116, 1501, 2677, 2943, 3754
BspMI ACCTGC 4 cut(s) 1119, 1381, 2104, 2493
BspPI GGATC 8 cut(s) 644, 716, 729, 1043, 1056, 1501, 2332, 3275
BspQI GCTCTTC 2 cut(s) 113, 2616
BspT104I TTCGAA 1 cut(s) 2896
BspT107I GGYRCC 4 cut(s) 1058, 2505, 2811, 3454
BspTI CTTAAG 1 cut(s) 3362
BsrDI GCAATG 4 cut(s) 467, 868, 2126, 3855
BsrFI RCCGGY 1 cut(s) 2094
BsrGI TGTACA 2 cut(s) 54, 3922
BssAI RCCGGY 1 cut(s) 2094
BssNI GRCGYC 2 cut(s) 2747, 2812
BssSI CACGAG 2 cut(s) 1063, 2966
BssT1I CCWWGG 8 cut(s) 301, 562, 1851, 2013, 2815, 3565, 3704, 3814
Bst2BI CACGAG 2 cut(s) 1063, 2966
Bst2UI CCWGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
Bst6I CTCTTC 5 cut(s) 113, 279, 1314, 1632, 2616
BstACI GRCGYC 2 cut(s) 2747, 2812
BstAFI CTTAAG 1 cut(s) 3362
BstAPI GCANNNNNTGC 1 cut(s) 3973
BstAUI TGTACA 2 cut(s) 54, 3922
BstBI TTCGAA 1 cut(s) 2896
BstC8I GCNNGC 8 cut(s) 125, 810, 1145, 1647, 2055, 2429, 3803, 3810
BstDSI CCRYGG 3 cut(s) 34, 2228, 2815
BstF5I GGATG 7 cut(s) 916, 940, 1552, 1690, 2173, 2998, 3130
BstFNI CGCG 3 cut(s) 92, 486, 3801
BstH2I RGCGCY 2 cut(s) 2815, 4020
BstHHI GCGC 6 cut(s) 861, 2814, 2916, 3779, 3801, 4019
BstMAI GTCTC 7 cut(s) 962, 1968, 2082, 2559, 2966, 3037, 3376
BstMCI CGRYCG 1 cut(s) 845
BstMWI GCNNNNNNNGC 8 cut(s) 240, 1646, 1790, 2437, 3802, 3805, 3941, 3973
BstNI CCWGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
BstNSI RCATGY 3 cut(s) 3050, 3812, 3929
BstPAI GACNNNNGTC 1 cut(s) 974
BstSCI CCNGG 6 cut(s) 264, 667, 988, 1305, 2250, 3134
BstSFI CTRYAG 6 cut(s) 1112, 1497, 2673, 2939, 3750, 3826
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 3 cut(s) 92, 486, 3801
BstV2I GAAGAC 2 cut(s) 80, 3648
BstX2I RGATCY 8 cut(s) 649, 721, 1048, 1493, 1561, 1665, 3280, 3876
BstXI CCANNNNNNTGG 3 cut(s) 1858, 2767, 3220
BstYI RGATCY 8 cut(s) 649, 721, 1048, 1493, 1561, 1665, 3280, 3876
BstZI CGGCCG 1 cut(s) 842
BsuI GTATCC 1 cut(s) 2764
BtgI CCRYGG 3 cut(s) 34, 2228, 2815
BtrI CACGTC 1 cut(s) 3166
BtsCI GGATG 7 cut(s) 916, 940, 1552, 1690, 2173, 2998, 3130
BtsI GCAGTG 5 cut(s) 1640, 2682, 2723, 3714, 3936
BtuMI TCGCGA 1 cut(s) 92
BveI ACCTGC 4 cut(s) 1119, 1381, 2104, 2493
Cac8I GCNNGC 8 cut(s) 125, 810, 1145, 1647, 2055, 2429, 3803, 3810
CaiI CAGNNNCTG 3 cut(s) 1111, 1496, 2938
CciI TCATGA 3 cut(s) 295, 964, 1396
CfoI GCGC 6 cut(s) 861, 2814, 2916, 3779, 3801, 4019
Cfr10I RCCGGY 1 cut(s) 2094
Cfr13I GGNCC 9 cut(s) 558, 559, 1811, 2097, 2225, 2335, 2701, 2935, 3210
CseI GACGC 3 cut(s) 91, 2736, 3642
DinI GGCGCC 1 cut(s) 2813
DraI TTTAAA 1 cut(s) 1480
DraIII CACNNNGTG 1 cut(s) 1448
EaeI YGGCCR 4 cut(s) 31, 397, 842, 2466
EagI CGGCCG 1 cut(s) 842
Eam1104I CTCTTC 5 cut(s) 113, 279, 1314, 1632, 2616
EarI CTCTTC 5 cut(s) 113, 279, 1314, 1632, 2616
EclXI CGGCCG 1 cut(s) 842
Eco130I CCWWGG 8 cut(s) 301, 562, 1851, 2013, 2815, 3565, 3704, 3814
Eco147I AGGCCT 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 562
Eco32I GATATC 2 cut(s) 1264, 3864
Eco47I GGWCC 6 cut(s) 1811, 2097, 2335, 2701, 2935, 3210
Eco47III AGCGCT 1 cut(s) 4018
Eco52I CGGCCG 1 cut(s) 842
Eco57I CTGAAG 4 cut(s) 303, 1197, 3216, 3509
Eco88I CYCGRG 2 cut(s) 3758, 3872
EcoO109I RGGNCCY 2 cut(s) 559, 2935
EcoRI GAATTC 1 cut(s) 3018
EcoRII CCWGG 6 cut(s) 264, 667, 988, 1305, 2250, 3134
EcoRV GATATC 2 cut(s) 1264, 3864
EcoT14I CCWWGG 8 cut(s) 301, 562, 1851, 2013, 2815, 3565, 3704, 3814
EcoT22I ATGCAT 3 cut(s) 547, 2128, 2191
EcoT38I GRGCYC 1 cut(s) 562
EgeI GGCGCC 1 cut(s) 2813
EheI GGCGCC 1 cut(s) 2813
ErhI CCWWGG 8 cut(s) 301, 562, 1851, 2013, 2815, 3565, 3704, 3814
FalI AAGNNNNNCTT 2 cut(s) 2375, 2407
FaqI GGGAC 2 cut(s) 502, 2525
FauI CCCGC 1 cut(s) 479
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 2 cut(s) 1399, 2563
FblI GTMKAC 1 cut(s) 1154
FokI GGATG 7 cut(s) 923, 947, 1539, 1697, 2180, 3005, 3137
FriOI GRGCYC 1 cut(s) 562
FspBI CTAG 7 cut(s) 158, 629, 678, 1452, 2247, 2361, 3518
GlaI GCGC 6 cut(s) 860, 2813, 2915, 3778, 3800, 4018
GsaI CCCAGC 2 cut(s) 594, 1590
GsuI CTGGAG 3 cut(s) 790, 957, 2802
HaeII RGCGCY 2 cut(s) 2815, 4020
HapII CCGG 4 cut(s) 659, 1389, 1843, 2095
HgaI GACGC 3 cut(s) 91, 2736, 3642
HhaI GCGC 6 cut(s) 861, 2814, 2916, 3779, 3801, 4019
Hin1I GRCGYC 2 cut(s) 2747, 2812
Hin6I GCGC 6 cut(s) 859, 2812, 2914, 3777, 3799, 4017
HinP1I GCGC 6 cut(s) 859, 2812, 2914, 3777, 3799, 4017
HincII GTYRAC 2 cut(s) 783, 1504
HindII GTYRAC 2 cut(s) 783, 1504
HindIII AAGCTT 5 cut(s) 125, 1269, 2446, 2899, 3305
HpaI GTTAAC 1 cut(s) 783
HpaII CCGG 4 cut(s) 659, 1389, 1843, 2095
HphI GGTGA 8 cut(s) 878, 1046, 2002, 2030, 2701, 3140, 3659, 3683
Hpy99I CGWCG 1 cut(s) 107
HpyCH4IV ACGT 2 cut(s) 2870, 3165
HpyF10VI GCNNNNNNNGC 8 cut(s) 240, 1646, 1790, 2437, 3802, 3805, 3941, 3973
HpySE526I ACGT 2 cut(s) 2870, 3165
Hsp92I GRCGYC 2 cut(s) 2747, 2812
HspAI GCGC 6 cut(s) 859, 2812, 2914, 3777, 3799, 4017
KasI GGCGCC 1 cut(s) 2811
Kpn2I TCCGGA 1 cut(s) 1388
KpnI GGTACC 1 cut(s) 2509
Ksp22I TGATCA 2 cut(s) 1399, 2563
KspAI GTTAAC 1 cut(s) 783
LguI GCTCTTC 2 cut(s) 113, 2616
LmnI GCTCC 5 cut(s) 511, 953, 1845, 2854, 3706
MaeI CTAG 7 cut(s) 158, 629, 678, 1452, 2247, 2361, 3518
MaeII ACGT 2 cut(s) 2870, 3165
MaeIII GTNAC 7 cut(s) 111, 308, 359, 3116, 3474, 3745, 3840
MflI RGATCY 8 cut(s) 649, 721, 1048, 1493, 1561, 1665, 3280, 3876
MhlI GDGCHC 1 cut(s) 562
MlsI TGGCCA 2 cut(s) 33, 2468
MluNI TGGCCA 2 cut(s) 33, 2468
Mly113I GGCGCC 1 cut(s) 2812
MlyI GAGTC 6 cut(s) 296, 964, 2084, 3035, 3050, 3770
MmeI TCCRAC 3 cut(s) 677, 1662, 1897
Mox20I TGGCCA 2 cut(s) 33, 2468
Mph1103I ATGCAT 3 cut(s) 547, 2128, 2191
MroI TCCGGA 1 cut(s) 1388
MroXI GAANNNNTTC 1 cut(s) 3549
MscI TGGCCA 2 cut(s) 33, 2468
MslI CAYNNNNRTG 5 cut(s) 540, 1199, 2107, 2468, 2734
Msp20I TGGCCA 2 cut(s) 33, 2468
MspCI CTTAAG 1 cut(s) 3362
MspI CCGG 4 cut(s) 659, 1389, 1843, 2095
MspR9I CCNGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
Mva1269I GAATGC 2 cut(s) 2364, 3680
MvaI CCWGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
MvnI CGCG 3 cut(s) 92, 486, 3801
MwoI GCNNNNNNNGC 8 cut(s) 240, 1646, 1790, 2437, 3802, 3805, 3941, 3973
NarI GGCGCC 1 cut(s) 2812
NcoI CCATGG 1 cut(s) 2815
NdeI CATATG 1 cut(s) 541
NmeAIII GCCGAG 2 cut(s) 820, 3375
NmuCI GTSAC 3 cut(s) 111, 308, 359
NruI TCGCGA 1 cut(s) 92
NsiI ATGCAT 3 cut(s) 547, 2128, 2191
NspI RCATGY 3 cut(s) 3050, 3812, 3929
NspV TTCGAA 1 cut(s) 2896
PaeI GCATGC 1 cut(s) 3812
PaeR7I CTCGAG 1 cut(s) 3758
PagI TCATGA 3 cut(s) 295, 964, 1396
PaqCI CACCTGC 1 cut(s) 1381
PceI AGGCCT 1 cut(s) 598
PciI ACATGT 1 cut(s) 3925
PciSI GCTCTTC 2 cut(s) 113, 2616
PctI GAATGC 2 cut(s) 2364, 3680
PdmI GAANNNNTTC 1 cut(s) 3549
PflMI CCANNNNNTGG 1 cut(s) 3566
PleI GAGTC 6 cut(s) 295, 964, 2083, 3034, 3049, 3769
PluTI GGCGCC 1 cut(s) 2815
PpsI GAGTC 6 cut(s) 295, 964, 2083, 3034, 3049, 3769
PpuMI RGGWCCY 1 cut(s) 2935
PscI ACATGT 1 cut(s) 3925
PshAI GACNNNNGTC 1 cut(s) 974
PshBI ATTAAT 1 cut(s) 2262
PsiI TTATAA 1 cut(s) 3111
Psp5II RGGWCCY 1 cut(s) 2935
Psp6I CCWGG 6 cut(s) 264, 667, 988, 1305, 2250, 3134
PspFI CCCAGC 2 cut(s) 590, 1586
PspGI CCWGG 6 cut(s) 264, 667, 988, 1305, 2250, 3134
PspOMI GGGCCC 1 cut(s) 558
PspPI GGNCC 9 cut(s) 558, 559, 1811, 2097, 2225, 2335, 2701, 2935, 3210
PspPPI RGGWCCY 1 cut(s) 2935
PspXI VCTCGAGB 1 cut(s) 3758
PstI CTGCAG 5 cut(s) 1116, 1501, 2677, 2943, 3754
PstNI CAGNNNCTG 3 cut(s) 1111, 1496, 2938
PsuI RGATCY 8 cut(s) 649, 721, 1048, 1493, 1561, 1665, 3280, 3876
RruI TCGCGA 1 cut(s) 92
RseI CAYNNNNRTG 5 cut(s) 540, 1199, 2107, 2468, 2734
SapI GCTCTTC 2 cut(s) 113, 2616
Sau96I GGNCC 9 cut(s) 558, 559, 1811, 2097, 2225, 2335, 2701, 2935, 3210
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 6 cut(s) 296, 964, 2084, 3035, 3050, 3770
ScrFI CCNGG 6 cut(s) 266, 669, 990, 1307, 2252, 3136
SduI GDGCHC 1 cut(s) 562
SfcI CTRYAG 6 cut(s) 1112, 1497, 2673, 2939, 3750, 3826
SfoI GGCGCC 1 cut(s) 2813
Sfr274I CTCGAG 1 cut(s) 3758
SfuI TTCGAA 1 cut(s) 2896
SinI GGWCC 6 cut(s) 1811, 2097, 2335, 2701, 2935, 3210
SlaI CTCGAG 1 cut(s) 3758
SmiMI CAYNNNNRTG 5 cut(s) 540, 1199, 2107, 2468, 2734
SphI GCATGC 1 cut(s) 3812
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 3 cut(s) 486, 997, 1141
SspDI GGCGCC 1 cut(s) 2811
SspI AATATT 2 cut(s) 2209, 3836
SspMI CTAG 7 cut(s) 158, 629, 678, 1452, 2247, 2361, 3518
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 6 cut(s) 264, 667, 988, 1305, 2250, 3134
StyI CCWWGG 8 cut(s) 301, 562, 1851, 2013, 2815, 3565, 3704, 3814
TaiI ACGT 2 cut(s) 2873, 3168
TaqI TCGA 5 cut(s) 47, 105, 2896, 3759, 3860
TatI WGTACW 5 cut(s) 54, 84, 571, 1367, 3922
TauI GCSGC 1 cut(s) 999
TseFI GTSAC 3 cut(s) 111, 308, 359
Tsp45I GTSAC 3 cut(s) 111, 308, 359
TspGWI ACGGA 5 cut(s) 51, 1211, 1241, 2070, 2327
Van91I CCANNNNNTGG 1 cut(s) 3566
Vha464I CTTAAG 1 cut(s) 3362
VpaK11BI GGWCC 6 cut(s) 1811, 2097, 2335, 2701, 2935, 3210
VspI ATTAAT 1 cut(s) 2262
XapI RAATTY 9 cut(s) 617, 703, 1289, 1606, 1944, 3018, 3153, 3241, 3326
XbaI TCTAGA 2 cut(s) 157, 3517
XceI RCATGY 3 cut(s) 3050, 3812, 3929
XhoI CTCGAG 1 cut(s) 3758
XmiI GTMKAC 1 cut(s) 1154
XmnI GAANNNNTTC 1 cut(s) 3549
XspI CTAG 7 cut(s) 158, 629, 678, 1452, 2247, 2361, 3518
ZrmI AGTACT 1 cut(s) 86
Zsp2I ATGCAT 3 cut(s) 547, 2128, 2191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.