pycom13g15030

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011033
Physical Location & Seq
Reverse (-)
10921576 .. 10926071
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g15030.3

Sequence Viewer

Length: 2919 bp
ATGCAGGTGAAGATTCTTCACACTCTAGATCATCAAAATATCCTCAAGTTTTACTGGTGGTATGAAACTTCTGCTCACTTGTGGTTGGTTTTGGAGTATTGCGTTGGGGGCAATTTGATGACACTATTAAACCAGGATAAGCAGCTACCTGAAGAGTCAATTCATGACCTTGGGTGTGACCTTGTTAGAGCTTTGCTTTGCCACAAGCGAAACGCGGGACACCTTGTTATATGGCTCCTGAGCTTTTTGAAGAAGGGGGTGTCCATTCATATGCATCTGATTTCTGGGCCCTTGGGTGTGTACTGTATGAGTGCTATGCTGGGAGATCCAACTCCACCTCTACCTGGAACTCCTAGCCGTTCTTTTGTCAATCTAATAAATTCTTTACTCATAAAGGATCCAGCAGAAAGAATACAGTGGCCTGAACTTTGTGGTCATGCTTTCTGGAGGACGAAGTTAACTTTAGTGCCTCTACCTCCTCAGCCTGCTTTTGATAATATGCTAGAGCAATATGCTCGGCCATGTCTGTCAGAGCGCAATAGTGATAAATCTTCCCAAATCAGAACTCCTAAATCTTGCCAAAAGGATGTTAAAGGAACTTTTAAGCAGGATGAGAATTCTATTTTGGGAGCAAACGGTCATGAGACTCCAGTCAAGGGTACACCTGGAAGCCGCAGAACTCAAGCAAAGGTTTCTGGCAGAGGGGTTAGTGAAAAACAAAAGGATCCTTCTGGTGCCACGAGGGGTGTGAATCTTTTGAGGCTCTCAAGAATTGCAAAACAGAACCTGCAGAGGGAAAATGAGAAGGAAAACTACCGCAGGCCAGTGTCTACTAATACTGAGAATGATTCTGAAGTCAAAATTGAGAACACGGACATGGAACTTGACTTTAATGAGAACACGGAAGATGACACACATGATGAATCCGATGGGTCTGATATCCAAGCTTGTCCACCTGACAACAAATTTTCAAGTCAGCACCAGGGTAAAATGGAAGAGATGGAAAACAACTTGAACCAATCAGATGCCCTGCCTGTGGTTAGTACACCTGCCTCAGATGAATCCGGAGTTCATGATCAGGAACCATCTCCCCAGAGTATTGAAATGGCTGCAATTCCACCCAGTGCCAGTCCTCAAATTAAAAATCAGAGATTTAAAGAAGGTTCTGGATCTGCAGTTGACTATGATTCTTCAAAATCTCCGAATAACCTTTCACAAGTTCTTTGGCATCCATCCGATCTCTCAGTCAGACCTGTGATGCCCAGCAGAAAATCTGACAAAAATTCGGAGGTAATTCCTTCTCTTCCCTTTGAGGCACTGCAAGCCTCTGACTTTGTGAAGATCTCTAAAGAGCAGTTGGATGCACTCAACAGTAAGATTATAGCCATTTTTAATGGGAACAGTAGCATTGGCGAGAAGCAGAATGTAATTAGATACCTTGAGATGTTAAGCAATAATGCTGATGCTGCAAATATCTTGACCAATGGACCTATCATGCTTTTGCTTGTTAAAATGCTCCGGCTGTCCAAGGCTTTGGCTTTGCGTGTCCAACTTGCTTCACTGGTTGGTTTGTTGATTAGACATTCTACTTTTATTCAAGATGATTTGGCAGATTCAGGAATTTTGGGTTCACTTGCTGATGGCCTTAGAGACAAGCAGGAAAAAGTGAGGAGGTTTTCTATGGCTGCTTTGGGAGAGTTGCTATTCTATATATCCACTCAAGGTGAGCATGCAGACAACAATCCAGCAGAGTCTCCATCAAAGGAAAGCCGGTCCACATCTGGGTGGCAGGTTTCAAATGCATTGCTTTCATTGGTGTCATCAATTTTACGAAAAGGAGAGGATGATTTAACTCAGTTATATGCATTGAGGACAATAGAAAATATTTGCAGTCAAGCAGGGCCGTGGGCAGCTCGTTTGACTAGCCAGGACATGATTAATAACCTGTGCTATTTATATAGGGCTGCTGGAAAGCAGGAGAGCATGAGGCTCACAGCAGGATCATGCTTGGTTCGTCTGGTTCGTTTTAACCCACCTAGCATTCAACCAGTAATAGAGAAGCTCTCCTTAAAGGACATAGCATCGGCACTTGTGAAGGGCAGTCTGCGTGAGCAGCAAATAAGCTTAAACCTCTTAAACATGGCCATGCTGGGAAGTCATGTGTTCACGAATGTTGGCAGGTACCTCCTCCCATTGATGGAGGATAAGAATCTTGTCCCAAGTCTTGTGTCTCTTATTGATCAGGGAAGTGAGGTTTTGAAGGGAAAGGCACTTGTTTTTGTTGCTCTTCTTTGTAAGAGCAGTAGGAGGTGGCTGCCACATTTTTTTTGCCATGCCAAGTTGCTTTCTGCGGTGGACAGGTTGGTGAAAGAGAAGGACCGTTATGTGCAGCAGTGTTTAGAGGCATCTGTGTGTGTTGTGGCGTCTGCTATACCCAGTTTACTGGATACTATTACTGGAGATATTCAGAAAATTATGGGAGGTAGGCGCCATGGGTATCAATCTCCCCTTAACAGTAGAGCTGCTCCAAAGACCAATGTTTATACGTTTCCCGTAGTTCTCCATCTTCTTCGAAGCTTGTCTTTTAAGCGCAAAGTGGTGAATGAACAGGTCCTGCAGCAGTTGGCAAATCTAATGAAACTCGTGGAGACGCCATTTCAGGGCAGGGATGACTTTCAGATTACCCTTCTCCGAATTCTTGAGTCTGTCTCAGAGGAGTCGCATGTGATTCTTGAAAGCCCTGAGATTTTTATTCGTGAGGTTCTCCCCAGTTTGGCTGTTCTTTATAAGGGTAATAAGGATGGTGATGCCAGGTTTCTGTGCTTGAAAATTTTGTTTGACGTGATGGTAATTTTTCTGAATGAACAATCTGAAGATCTATCAACAAAGGTCCAAAGAACTGGAATCCATATCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

973

Amino Acids

108.23

Weight (kDa)

7.09

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2787
AarI CACCTGC 1 cut(s) 1057
Acc36I ACCTGC 4 cut(s) 795, 1057, 1780, 2169
Acc65I GGTACC 1 cut(s) 2181
AccB1I GGYRCC 3 cut(s) 734, 2181, 2487
AccI GTMKAC 1 cut(s) 830
AccII CGCG 1 cut(s) 215
AccIII TCCGGA 1 cut(s) 1064
AciI CCGC 4 cut(s) 215, 673, 817, 2351
AclWI GGATC 7 cut(s) 320, 392, 405, 719, 732, 1177, 2008
AcoI YGGCCR 2 cut(s) 518, 2142
AcsI RAATTY 7 cut(s) 379, 616, 965, 1282, 1620, 2694, 2829
AcuI CTGAAG 3 cut(s) 171, 873, 2892
AcyI GRCGYC 3 cut(s) 2423, 2488, 2651
AdeI CACNNNGTG 1 cut(s) 1124
AfaI GTAC 4 cut(s) 302, 661, 1045, 2183
AfiI CCNNNNNNNGG 8 cut(s) 344, 656, 793, 1036, 1782, 2197, 2660, 2773
AjiI CACGTC 1 cut(s) 2842
AjnI CCWGG 6 cut(s) 132, 343, 664, 981, 1926, 2810
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 9 cut(s) 145, 191, 243, 947, 1913, 2062, 2124, 2522, 2577
AluI AGCT 9 cut(s) 145, 191, 243, 947, 1913, 2062, 2124, 2522, 2577
Alw26I GTCTC 6 cut(s) 638, 1644, 1758, 2235, 2642, 2713
AlwI GGATC 7 cut(s) 320, 392, 405, 719, 732, 1177, 2008
AlwNI CAGNNNCTG 2 cut(s) 787, 2614
Aor13HI TCCGGA 1 cut(s) 1064
AoxI GGCC 7 cut(s) 287, 419, 518, 821, 1642, 1901, 2142
ApaI GGGCCC 1 cut(s) 291
ApoI RAATTY 7 cut(s) 379, 616, 965, 1282, 1620, 2694, 2829
AseI ATTAAT 1 cut(s) 1938
Asp718I GGTACC 1 cut(s) 2181
AspLEI GCGC 3 cut(s) 537, 2490, 2592
AspS9I GGNCC 8 cut(s) 287, 288, 1487, 1773, 1901, 2377, 2611, 2890
AsuHPI GGTGA 5 cut(s) 19, 1736, 2377, 2611, 2816
AsuII TTCGAA 1 cut(s) 2572
AvaII GGWCC 5 cut(s) 1487, 1773, 2377, 2611, 2890
BaeGI GKGCMC 1 cut(s) 291
BalI TGGCCA 1 cut(s) 2144
BamHI GGATCC 2 cut(s) 397, 724
BanI GGYRCC 3 cut(s) 734, 2181, 2487
BanII GRGCYC 1 cut(s) 291
BarI GAAGNNNNNNTAC 2 cut(s) 797, 829
BauI CACGAG 2 cut(s) 739, 2642
BbvCI CCTCAGC 1 cut(s) 480
BceAI ACGGC 2 cut(s) 342, 1888
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
BciVI GTATCC 1 cut(s) 2440
BclI TGATCA 2 cut(s) 1075, 2239
BcoDI GTCTC 6 cut(s) 638, 1644, 1758, 2235, 2642, 2713
BfaI CTAG 5 cut(s) 26, 354, 503, 1923, 2037
BfmI CTRYAG 3 cut(s) 788, 1173, 2615
BfoI RGCGCY 1 cut(s) 2491
BfuAI ACCTGC 4 cut(s) 795, 1057, 1780, 2169
BfuI GTATCC 1 cut(s) 2440
BglII AGATCT 2 cut(s) 1341, 2875
Bme1390I CCNGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
Bme18I GGWCC 5 cut(s) 1487, 1773, 2377, 2611, 2890
BmgBI CACGTC 1 cut(s) 2842
BmgT120I GGNCC 8 cut(s) 287, 288, 1487, 1773, 1901, 2377, 2611, 2890
BmiI GGNNCC 8 cut(s) 236, 289, 399, 726, 736, 1083, 2183, 2489
BmrFI CCNGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
BmrI ACTGGG 3 cut(s) 1116, 2430, 2763
BmsI GCATC 9 cut(s) 283, 1015, 1237, 1248, 1351, 1453, 2090, 2414, 2797
BmuI ACTGGG 3 cut(s) 1116, 2430, 2763
BoxI GACNNNNGTC 1 cut(s) 650
BplI GAGNNNNNCTC 4 cut(s) 2048, 2080, 2693, 2725
BpmI CTGGAG 3 cut(s) 466, 633, 2478
Bpu10I CCTNAGC 2 cut(s) 239, 480
Bpu14I TTCGAA 1 cut(s) 2572
BpuEI CTTGAG 6 cut(s) 29, 666, 751, 1460, 1704, 2720
BsaBI GATNNNNATC 1 cut(s) 2208
BsaHI GRCGYC 3 cut(s) 2423, 2488, 2651
BsaJI CCNNGG 6 cut(s) 169, 291, 982, 1527, 1904, 2491
BsaWI WCCGGW 1 cut(s) 1064
Bsc4I CCNNNNNNNGG 8 cut(s) 344, 656, 793, 1036, 1782, 2197, 2660, 2773
Bse118I RCCGGY 1 cut(s) 1770
Bse3DI GCAATG 1 cut(s) 1802
Bse8I GATNNNNATC 1 cut(s) 2208
BseAI TCCGGA 1 cut(s) 1064
BseBI CCWGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
BseDI CCNNGG 6 cut(s) 169, 291, 982, 1527, 1904, 2491
BseGI GGATG 8 cut(s) 592, 616, 1228, 1232, 1366, 1849, 2674, 2806
BseJI GATNNNNATC 1 cut(s) 2208
BseLI CCNNNNNNNGG 8 cut(s) 344, 656, 793, 1036, 1782, 2197, 2660, 2773
BseMI GCAATG 1 cut(s) 1802
BseMII CTCAG 8 cut(s) 230, 494, 831, 1068, 1257, 1868, 2724, 2733
BseRI GAGGAG 4 cut(s) 468, 1684, 2177, 2729
BseSI GKGCMC 1 cut(s) 291
BseYI CCCAGC 3 cut(s) 319, 1262, 2149
BsgI GTGCAG 1 cut(s) 2408
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 7 cut(s) 289, 421, 520, 823, 1644, 1903, 2144
BshNI GGYRCC 3 cut(s) 734, 2181, 2487
BsiSI CCGG 3 cut(s) 1065, 1519, 1771
BslFI GGGAC 2 cut(s) 231, 2201
BslI CCNNNNNNNGG 8 cut(s) 344, 656, 793, 1036, 1782, 2197, 2660, 2773
BsmAI GTCTC 6 cut(s) 638, 1644, 1758, 2235, 2642, 2713
BsmBI CGTCTC 1 cut(s) 2642
BsmFI GGGAC 2 cut(s) 231, 2201
BsmI GAATGC 1 cut(s) 2040
BsnI GGCC 7 cut(s) 289, 421, 520, 823, 1644, 1903, 2144
Bsp119I TTCGAA 1 cut(s) 2572
Bsp120I GGGCCC 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 1064
Bsp19I CCATGG 1 cut(s) 2491
BspACI CCGC 4 cut(s) 215, 673, 817, 2351
BspANI GGCC 7 cut(s) 289, 421, 520, 823, 1644, 1903, 2144
BspCNI CTCAG 8 cut(s) 231, 493, 832, 1067, 1256, 1867, 2723, 2734
BspEI TCCGGA 1 cut(s) 1064
BspFNI CGCG 1 cut(s) 215
BspHI TCATGA 3 cut(s) 163, 640, 1072
BspLI GGNNCC 8 cut(s) 236, 289, 399, 726, 736, 1083, 2183, 2489
BspMAI CTGCAG 3 cut(s) 792, 1177, 2619
BspMI ACCTGC 4 cut(s) 795, 1057, 1780, 2169
BspPI GGATC 7 cut(s) 320, 392, 405, 719, 732, 1177, 2008
BspQI GCTCTTC 1 cut(s) 2292
BspT104I TTCGAA 1 cut(s) 2572
BspT107I GGYRCC 3 cut(s) 734, 2181, 2487
BsrDI GCAATG 1 cut(s) 1802
BsrFI RCCGGY 1 cut(s) 1770
BssAI RCCGGY 1 cut(s) 1770
BssECI CCNNGG 6 cut(s) 169, 291, 982, 1527, 1904, 2491
BssNI GRCGYC 3 cut(s) 2423, 2488, 2651
BssSI CACGAG 2 cut(s) 739, 2642
BssT1I CCWWGG 4 cut(s) 169, 291, 1527, 2491
Bst2BI CACGAG 2 cut(s) 739, 2642
Bst2UI CCWGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
Bst4CI ACNGT 7 cut(s) 305, 417, 638, 1373, 1403, 2381, 2516
Bst6I CTCTTC 4 cut(s) 147, 990, 1308, 2292
BstACI GRCGYC 3 cut(s) 2423, 2488, 2651
BstBI TTCGAA 1 cut(s) 2572
BstC8I GCNNGC 4 cut(s) 486, 821, 1323, 1731
BstDEI CTNAG 9 cut(s) 239, 480, 840, 1054, 1243, 1646, 1854, 2710, 2742
BstDSI CCRYGG 2 cut(s) 1904, 2491
BstF5I GGATG 8 cut(s) 592, 616, 1228, 1232, 1366, 1849, 2674, 2806
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 1 cut(s) 2491
BstHHI GCGC 3 cut(s) 537, 2490, 2592
BstMAI GTCTC 6 cut(s) 638, 1644, 1758, 2235, 2642, 2713
BstMWI GCNNNNNNNGC 4 cut(s) 108, 1322, 1466, 2113
BstNI CCWGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
BstNSI RCATGY 2 cut(s) 1733, 2726
BstPAI GACNNNNGTC 1 cut(s) 650
BstSCI CCNGG 6 cut(s) 132, 343, 664, 981, 1926, 2810
BstSFI CTRYAG 3 cut(s) 788, 1173, 2615
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 215
BstX2I RGATCY 6 cut(s) 325, 397, 724, 1169, 1341, 2875
BstXI CCANNNNNNTGG 3 cut(s) 1534, 2443, 2900
BstYI RGATCY 6 cut(s) 325, 397, 724, 1169, 1341, 2875
BsuI GTATCC 1 cut(s) 2440
BsuRI GGCC 7 cut(s) 289, 421, 520, 823, 1644, 1903, 2144
BtgI CCRYGG 2 cut(s) 1904, 2491
BtrI CACGTC 1 cut(s) 2842
BtsCI GGATG 8 cut(s) 592, 616, 1228, 1232, 1366, 1849, 2674, 2806
BtsI GCAGTG 2 cut(s) 1316, 2399
BtsIMutI CAGTG 6 cut(s) 422, 831, 1129, 1316, 1559, 2399
BveI ACCTGC 4 cut(s) 795, 1057, 1780, 2169
Cac8I GCNNGC 4 cut(s) 486, 821, 1323, 1731
CaiI CAGNNNCTG 2 cut(s) 787, 2614
CciI TCATGA 3 cut(s) 163, 640, 1072
CfoI GCGC 3 cut(s) 537, 2490, 2592
Cfr10I RCCGGY 1 cut(s) 1770
Cfr13I GGNCC 8 cut(s) 287, 288, 1487, 1773, 1901, 2377, 2611, 2890
CseI GACGC 2 cut(s) 2412, 2659
Csp6I GTAC 4 cut(s) 301, 660, 1044, 2182
CviQI GTAC 4 cut(s) 301, 660, 1044, 2182
DdeI CTNAG 9 cut(s) 239, 480, 840, 1054, 1243, 1646, 1854, 2710, 2742
DinI GGCGCC 1 cut(s) 2489
DraI TTTAAA 1 cut(s) 1156
DraIII CACNNNGTG 1 cut(s) 1124
EaeI YGGCCR 2 cut(s) 518, 2142
Eam1104I CTCTTC 4 cut(s) 147, 990, 1308, 2292
EarI CTCTTC 4 cut(s) 147, 990, 1308, 2292
Eco130I CCWWGG 4 cut(s) 169, 291, 1527, 2491
Eco24I GRGCYC 1 cut(s) 291
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 5 cut(s) 1487, 1773, 2377, 2611, 2890
Eco57I CTGAAG 3 cut(s) 171, 873, 2892
EcoO109I RGGNCCY 2 cut(s) 288, 2611
EcoRI GAATTC 2 cut(s) 616, 2694
EcoRII CCWGG 6 cut(s) 132, 343, 664, 981, 1926, 2810
EcoRV GATATC 1 cut(s) 940
EcoT14I CCWWGG 4 cut(s) 169, 291, 1527, 2491
EcoT22I ATGCAT 3 cut(s) 276, 1804, 1867
EcoT38I GRGCYC 1 cut(s) 291
EgeI GGCGCC 1 cut(s) 2489
EheI GGCGCC 1 cut(s) 2489
ErhI CCWWGG 4 cut(s) 169, 291, 1527, 2491
Esp3I CGTCTC 1 cut(s) 2642
FalI AAGNNNNNCTT 4 cut(s) 2051, 2083, 2566, 2598
FaqI GGGAC 2 cut(s) 231, 2201
FauI CCCGC 1 cut(s) 208
FauNDI CATATG 1 cut(s) 270
FbaI TGATCA 2 cut(s) 1075, 2239
FblI GTMKAC 1 cut(s) 830
FokI GGATG 8 cut(s) 599, 623, 1215, 1219, 1373, 1856, 2681, 2813
FriOI GRGCYC 1 cut(s) 291
FspBI CTAG 5 cut(s) 26, 354, 503, 1923, 2037
GlaI GCGC 3 cut(s) 536, 2489, 2591
GsaI CCCAGC 3 cut(s) 323, 1266, 2153
GsuI CTGGAG 3 cut(s) 466, 633, 2478
HaeII RGCGCY 1 cut(s) 2491
HaeIII GGCC 7 cut(s) 289, 421, 520, 823, 1644, 1903, 2144
HapII CCGG 3 cut(s) 1065, 1519, 1771
HgaI GACGC 2 cut(s) 2412, 2659
HhaI GCGC 3 cut(s) 537, 2490, 2592
Hin1I GRCGYC 3 cut(s) 2423, 2488, 2651
Hin6I GCGC 3 cut(s) 535, 2488, 2590
HinP1I GCGC 3 cut(s) 535, 2488, 2590
HincII GTYRAC 2 cut(s) 459, 1180
HindII GTYRAC 2 cut(s) 459, 1180
HindIII AAGCTT 3 cut(s) 945, 2122, 2575
HpaI GTTAAC 1 cut(s) 459
HpaII CCGG 3 cut(s) 1065, 1519, 1771
HphI GGTGA 5 cut(s) 19, 1736, 2377, 2611, 2816
HpyAV CCTTC 9 cut(s) 247, 738, 799, 1154, 1308, 2089, 2254, 2368, 2696
HpyCH4III ACNGT 7 cut(s) 305, 417, 638, 1373, 1403, 2381, 2516
HpyCH4IV ACGT 2 cut(s) 2546, 2841
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 1322, 1466, 2113
HpyF3I CTNAG 9 cut(s) 239, 480, 840, 1054, 1243, 1646, 1854, 2710, 2742
HpySE526I ACGT 2 cut(s) 2546, 2841
Hsp92I GRCGYC 3 cut(s) 2423, 2488, 2651
HspAI GCGC 3 cut(s) 535, 2488, 2590
KasI GGCGCC 1 cut(s) 2487
Kpn2I TCCGGA 1 cut(s) 1064
KpnI GGTACC 1 cut(s) 2185
Ksp22I TGATCA 2 cut(s) 1075, 2239
KspAI GTTAAC 1 cut(s) 459
LguI GCTCTTC 1 cut(s) 2292
LmnI GCTCC 4 cut(s) 240, 629, 1521, 2530
LweI GCATC 9 cut(s) 283, 1015, 1237, 1248, 1351, 1453, 2090, 2414, 2797
MaeI CTAG 5 cut(s) 26, 354, 503, 1923, 2037
MaeII ACGT 2 cut(s) 2546, 2841
MaeIII GTNAC 1 cut(s) 176
MflI RGATCY 6 cut(s) 325, 397, 724, 1169, 1341, 2875
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 1 cut(s) 2144
MluNI TGGCCA 1 cut(s) 2144
Mly113I GGCGCC 1 cut(s) 2488
MlyI GAGTC 5 cut(s) 164, 640, 1760, 2711, 2726
MmeI TCCRAC 3 cut(s) 353, 1338, 1573
Mox20I TGGCCA 1 cut(s) 2144
Mph1103I ATGCAT 3 cut(s) 276, 1804, 1867
MroI TCCGGA 1 cut(s) 1064
MscI TGGCCA 1 cut(s) 2144
MslI CAYNNNNRTG 5 cut(s) 269, 875, 1783, 2144, 2410
Msp20I TGGCCA 1 cut(s) 2144
MspI CCGG 3 cut(s) 1065, 1519, 1771
MspR9I CCNGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
Mva1269I GAATGC 1 cut(s) 2040
MvaI CCWGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 4 cut(s) 108, 1322, 1466, 2113
NarI GGCGCC 1 cut(s) 2488
NcoI CCATGG 1 cut(s) 2491
NdeI CATATG 1 cut(s) 270
NlaIV GGNNCC 8 cut(s) 236, 289, 399, 726, 736, 1083, 2183, 2489
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 1 cut(s) 176
NsiI ATGCAT 3 cut(s) 276, 1804, 1867
NspI RCATGY 2 cut(s) 1733, 2726
NspV TTCGAA 1 cut(s) 2572
PaeI GCATGC 1 cut(s) 1733
PagI TCATGA 3 cut(s) 163, 640, 1072
PaqCI CACCTGC 1 cut(s) 1057
PciSI GCTCTTC 1 cut(s) 2292
PcsI WCGNNNNNNNCGW 1 cut(s) 2020
PctI GAATGC 1 cut(s) 2040
PleI GAGTC 5 cut(s) 163, 640, 1759, 2710, 2725
PluTI GGCGCC 1 cut(s) 2491
PpsI GAGTC 5 cut(s) 163, 640, 1759, 2710, 2725
PpuMI RGGWCCY 1 cut(s) 2611
PshAI GACNNNNGTC 1 cut(s) 650
PshBI ATTAAT 1 cut(s) 1938
PsiI TTATAA 1 cut(s) 2787
Psp5II RGGWCCY 1 cut(s) 2611
Psp6I CCWGG 6 cut(s) 132, 343, 664, 981, 1926, 2810
PspFI CCCAGC 3 cut(s) 319, 1262, 2149
PspGI CCWGG 6 cut(s) 132, 343, 664, 981, 1926, 2810
PspN4I GGNNCC 8 cut(s) 236, 289, 399, 726, 736, 1083, 2183, 2489
PspOMI GGGCCC 1 cut(s) 287
PspPI GGNCC 8 cut(s) 287, 288, 1487, 1773, 1901, 2377, 2611, 2890
PspPPI RGGWCCY 1 cut(s) 2611
PstI CTGCAG 3 cut(s) 792, 1177, 2619
PstNI CAGNNNCTG 2 cut(s) 787, 2614
PsuI RGATCY 6 cut(s) 325, 397, 724, 1169, 1341, 2875
RsaI GTAC 4 cut(s) 302, 661, 1045, 2183
RsaNI GTAC 4 cut(s) 301, 660, 1044, 2182
RseI CAYNNNNRTG 5 cut(s) 269, 875, 1783, 2144, 2410
SapI GCTCTTC 1 cut(s) 2292
Sau96I GGNCC 8 cut(s) 287, 288, 1487, 1773, 1901, 2377, 2611, 2890
SchI GAGTC 5 cut(s) 164, 640, 1760, 2711, 2726
ScrFI CCNGG 6 cut(s) 134, 345, 666, 983, 1928, 2812
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 9 cut(s) 283, 1015, 1237, 1248, 1351, 1453, 2090, 2414, 2797
SfcI CTRYAG 3 cut(s) 788, 1173, 2615
SfoI GGCGCC 1 cut(s) 2489
SfuI TTCGAA 1 cut(s) 2572
SinI GGWCC 5 cut(s) 1487, 1773, 2377, 2611, 2890
SmiMI CAYNNNNRTG 5 cut(s) 269, 875, 1783, 2144, 2410
SmlI CTYRAG 6 cut(s) 44, 681, 766, 1439, 1719, 2699
SmoI CTYRAG 6 cut(s) 44, 681, 766, 1439, 1719, 2699
SphI GCATGC 1 cut(s) 1733
SsiI CCGC 4 cut(s) 215, 673, 817, 2351
SspDI GGCGCC 1 cut(s) 2487
SspI AATATT 1 cut(s) 1885
SspMI CTAG 5 cut(s) 26, 354, 503, 1923, 2037
StyD4I CCNGG 6 cut(s) 132, 343, 664, 981, 1926, 2810
StyI CCWWGG 4 cut(s) 169, 291, 1527, 2491
TaaI ACNGT 7 cut(s) 305, 417, 638, 1373, 1403, 2381, 2516
TaiI ACGT 2 cut(s) 2549, 2844
TaqI TCGA 1 cut(s) 2572
TatI WGTACW 2 cut(s) 300, 1043
TauI GCSGC 1 cut(s) 675
TscAI CASTG 6 cut(s) 422, 831, 1129, 1323, 1566, 2399
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspGWI ACGGA 2 cut(s) 887, 917
TspRI CASTG 6 cut(s) 422, 831, 1129, 1323, 1566, 2399
VpaK11BI GGWCC 5 cut(s) 1487, 1773, 2377, 2611, 2890
VspI ATTAAT 1 cut(s) 1938
XapI RAATTY 7 cut(s) 379, 616, 965, 1282, 1620, 2694, 2829
XbaI TCTAGA 1 cut(s) 25
XceI RCATGY 2 cut(s) 1733, 2726
XmiI GTMKAC 1 cut(s) 830
XspI CTAG 5 cut(s) 26, 354, 503, 1923, 2037
Zsp2I ATGCAT 3 cut(s) 276, 1804, 1867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.