Rw4G019720

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011033
Physical Location & Seq
Forward (+)
43242379 .. 43248171
5793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019720.1

Sequence Viewer

Length: 4020 bp
ATGAACCAGTACCACATATACGAGGCCATCGGCCGTGGAAAGTGCTCGACTGTGTACAAAGGGAGGAAGAAGAAGACGATTGAGTACTTCGCCATTAAGAGCGTCGAGAAATCTCAGAAGAGCAAGCTTCTTCAGGAAGTTAAGATTCTTCATACCCTAGATCATCAGAATATTCTCAAGTTTTATTGGTGGTACGAAACTTCTGCTCACTTGTGGTTAGTTCTGGAGTATTGCGTTGGCGGGAATTTGATGACATTGTTAGACCAGGATCGTCAGCTACCTGAAGAGTCGATTCATGACCTTGGGTGGGACCTTGTTAGAGCTTTGCTGTTTTTACATTCGAAGGGAATCATATACTGTGATTTGAAACCATCGAATATCCTGTTGGATGAAAATGGCCGTACAAAGCTCTGTGATTTTGGATTGGCTAGAAAGCTGAGCGAAATATCACAAACCACTTCTTCCTCGTTGCCACAAGCAAAACGTGGAACACCTTGTTATATGGCTCCTGAGCTTTTTGAAGACGGGGGTGTCCATTCATATGCATCTGATTTCTGGGCCCTTGGTTGTGTACTATATGAGTGCTATGCGGGGAAGCCTCCCTTTGTGGGGAGAGAATTTACTCAGCTAGTGAAGTCCATCCTCTCAGATCCAATACCTCCTCTACCTGGCACTCCTAGTCGTCCTTTTGTCAATCTAATAAATTCTTTACTCATCAAGGATCCAGCTGAACGAATACAGTGGCCTGAACTTTGTGGTCATGCTTTCTGGAGAACCAAGTTAAATCTAGTGCCTCTACCTCCTCAGCCTGCTTTTGCAAATATGCTAGAGCAATATGCTAAGCCATTGTCAATACGTAATGGTGAGAACAGGACTCCTAAATCTCGCCAAAACGAGGTTAAAGGAGCTTCAAAGCAGGATGAGAATTCTGTAGTAGGATTGAAAGGCCATGGAACTCCAGTCAGGGGCACACCTGGAAGTCGGAGAACTCAAGCAAAGGTATCTGGCAGAGCAGTTGATGAAAAGCAAAAGGATCCTTCTGGTACTACCAGGGGTGTGAATCTTTTGAGACTCTCAAGAATAGCAAAGCAGAACTTGCAGAGGGAAAATGAGAAAGAGAATTACCGGAGGCTAGTGTCTACTAATTCTGAGAATGATTCAGAAGTCAAAATTGAGAACACTGATATGGAACTTGATTTTAATGAAAACACTGAAGATGATGCACAGGATGAATCTGATGGGCCAGATAACCAAGCTTCACTGGATGACAAATCTTCAAGTCAAAATCAGGAGCAGGTTAAAGTGGAAGAGATGGAAAATAACATAAACCAGTCAGATACCTCATCTGTGGTAAATGCTTCTGCATCAAATGAATCAACAGCATGTGATCAGGAATCACCACAGAATATTGAAGTGGCTGCAATTCCATGCAGTGCCAGTCCTCAGATAAAAAATCAGAGATTTAAGGAAGGCTCTGGATCCGCAGTTGACTATGATTCTGCAAAATCGTCAAATAACCTTTCAGAGGTTCTTTGGCATCCATCTGATCTTTCAGTCAGACCTGTAATGCCTAACAGAAAAGCTGATAAAACTTCGGAGGTAATTCCTTCACTTCCCTTTGAGGCACTGCAAGCTTCTGATTTTGTGAAGATGTCTAAAGAGCAATTGGATGCACTTATTAATAAGATTATAGCCATTTTTAATGGGAACATTAGCATTGGGGAGAAGCAGAATGCACTTAGATACCTTGAGATGTTAACCAACAATGCGGATGCTGCTAATATCTTGACCAATGGACCCATCATGCTTATCCTTGTTAAAATGCTTCGACAGACCAAGGCTTTGGCTTTGCGTGTCCAAGTTGCTTCACTGATTGGTTTATTGATTCGACATTCTACTTTTATTCATGATGATTTGGCAAATTCTGGAATTTTGGGTTCACTGAGCGATGGCCTTGGAGACAGGCAGGAAAAAGTGAGGAGATTTTCTATGGCTGCTTTAGGTGAGCTGCTATTCTATATTTCAACTCAAAGTGAGCAAGTTGGAGATAACAATCCTACTGAGTCTCCATCTAAGGAAAGCCGGTCCACGTCTGGTTGGCAGGTCTCCAATTTATTGCTTTCCAAGGTGGCATCAATTTTACGAAAAGGAGAGGATGATTTAACTCAGCTTTATGCATTGAGGACGATTGAAAATATCTGTAGCCAAGCAGGGCACTGGGCAGCTCGTTTGAACAGCCAGGGCGTGATTAATAACCTGTGCTATATATATAGGGCTGCTGGGAAACAGGAGAGCATGAGACTTACAGCAGGATCATGTTTGGTCCGTCTTGTTCGTTTCAATCCACCTAGCATTCAGCAAGTAATTGATATTCTTTCTTTAAAGGAAATAGCATCTGCAATTATCAAGGGCAGTCTGAGGGAGCAGCAAATCAGCTTAAACCTCTTGAACATGGCCATGCTTGGAAGTCACAATTTCACAAACTTTGGCAGGCACCTCCTGCCACTGATGGAGGATAAGAATATTGTTCCAAGTCTTGTGTCTCTTATTGAGCAGGGAAGTGAAGTTTTGAAAGGAAAAGCGCTTGTTTTTGTGCTGCCACATTTTTTTTGCAATGCCAGGTTACTGTCTATGGTGGACAGGCTGGCAAAAGAGAAAGATCAGTATGTGAGGCAGTGTTTAGATGCATTTGTTTGTGTTGTGGCTTCTGTTATACCTGGTTTACTGGATACTATAACTGGTGACATTCAGCAATCAATGGGAAGGCGCCATGTACATCTATCTCCCCTGAACAGTAGAGCTACTCCTAAGACCAACATTCATATGCTCCCTGTAGTTCTCCATCTTCTTCGAAGCTCGTCTTTTAAGCATAAGGTGGTGAGTGATCAGGTCCTGCAGCAGTTGGCAAATTTAATAAAAATCGTGGAAACACCATTTCAGGGCAGGGATGACTTTCAAATCACTCTTCTTCGAATTCTTGAATCTGTTACCGAGGAGTCCCTTGTAATTCTCAAAAGCCCTGATATTTTTACTCATGAGGTTCTCCCCAGTTTGGCTGTTCTGTATAAGGGCAACAAGGATGGTGACGCCAGGTTTCTGTGTTTGAAAATCTTGTTTGATGTGATGGTAATTTTTCTTAATGAACAATCTGAAGATGAGCAAAGGTCCAAAGAATTGCAATCCATATCCAATACAAACTTTCTTCCTCTCTACCCTTCCCTGATTGAAGACGAAGATCCAATCCCCTTGTACGCACAAAAGCTGCTTGTGATGCTGATTGATTATAATTACATCAAAATTGCAGACATTCTACATCTGAAGATAGTCTCGCAATGCTTTGAGTTTTTGCTGGGCGATCTCTCAAGTGCAAATGTGAACAATGTTATGCTATGCCTGGCTCTGACATCTGCTCCTGAAATGGAAACCAAGTTACTTTCTCAGCTGAGGCTTGTATATGCAAAAGATATGGAAGATTTTTTGGAACCAACTCTTGGTCTGTGTAGGGCTTTCCTTTTACGTTCAGTGAGTGGTAGAAAGGGCTTCACCTATTCAACAGAACCTGCTCTTTTGGGTGATGTGTCTTCTGAGCCAAGTGGTGATCAGCAGCAAGGTATAAGAGATATCATGGACTTTGCCAGCAATATTGGTGTATTGCTAGAGTTAAGTAGGTCTCATGTAGGAAATGTGGCAGATATAGCTTCAGAATGTGTTGTCTTGTTGCTCAAGGCAGCTCCAAGGGAAGGTACTGCTGGTCTACTAACTAATCTTCCAAAAGTTACTGCAATGCTTGAGTCTTGGCGCAGAGGCACATCTCATTTGTTGGTGCAGCGAGTGCTACATGCACTTGGTTATGCTTGTAGGCAGTATTTGTTACATGCAATGATAATGTCGATATCGATACCTGAGATTCCAAGAATTGAGGGCATAGTTTCTGAGCTTAAAAGTTCAGGTGTACCTGCTTTAGCCACTGCTGCTTTTCATGTGGCTGTGGAGTTGCAGCGGCTTCCTCGCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1339

Amino Acids

149.0

Weight (kDa)

6.32

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 256 8.5e-64 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 251 6e-38 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 66 - 151 4e-07 ABC1 atypical kinase-like domain
HEAT_ULK4 PF23606 908 - 1100 2e-71 Serine/threonine-protein kinase ULK4-like, HEAT repeats
ARM_RUK PF24970 1107 - 1338 1.6e-104 RUNKEL ARM-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3285
AasI GACNNNNNNGTC 1 cut(s) 530
Acc36I ACCTGC 4 cut(s) 1285, 2092, 3568, 3964
AccB1I GGYRCC 2 cut(s) 2493, 2766
AccB7I CCANNNNNTGG 1 cut(s) 3491
AccI GTMKAC 2 cut(s) 1139, 3754
AciI CCGC 5 cut(s) 240, 590, 1482, 1769, 4000
AcoI YGGCCR 3 cut(s) 31, 397, 2454
AcsI RAATTY 8 cut(s) 244, 617, 703, 925, 1921, 1929, 2908, 2973
AcuI CTGAAG 6 cut(s) 116, 303, 1233, 3171, 3338, 3684
AcyI GRCGYC 2 cut(s) 2767, 3087
AfeI AGCGCT 1 cut(s) 2583
AjiI CACGTC 1 cut(s) 2091
AjnI CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2618, 2716, 3089, 3393
AjuI GAANNNNNNNTTGG 4 cut(s) 368, 400, 3474, 3506
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3331, 3675
AlwNI CAGNNNCTG 2 cut(s) 2893, 3560
Aor51HI AGCGCT 1 cut(s) 2583
AoxI GGCC 9 cut(s) 24, 31, 397, 558, 743, 946, 1241, 1951, 2454
ApaI GGGCCC 1 cut(s) 562
ApoI RAATTY 8 cut(s) 244, 617, 703, 925, 1921, 1929, 2908, 2973
ArsI GACNNNNNNTTYG 2 cut(s) 1825, 1857
AseI ATTAAT 2 cut(s) 1680, 2250
AspLEI GCGC 3 cut(s) 2584, 2769, 3801
AspS9I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2890, 3165
AsuHPI GGTGA 9 cut(s) 875, 1389, 2015, 2753, 2890, 3095, 3535, 3584, 3608
AsuII TTCGAA 3 cut(s) 341, 2851, 2971
AvaII GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2890, 3165
BaeGI GKGCMC 3 cut(s) 562, 971, 2217
BalI TGGCCA 1 cut(s) 2456
BamHI GGATCC 3 cut(s) 721, 1033, 1478
BanI GGYRCC 2 cut(s) 2493, 2766
BanII GRGCYC 1 cut(s) 562
BbsI GAAGAC 4 cut(s) 80, 528, 3234, 3573
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 2 cut(s) 804, 3443
BceAI ACGGC 2 cut(s) 18, 384
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
BciVI GTATCC 1 cut(s) 2722
BclI TGATCA 3 cut(s) 1387, 2884, 3598
BcoDI GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3331, 3675
BfaI CTAG 9 cut(s) 158, 429, 629, 678, 788, 827, 1133, 2349, 3656
BfmI CTRYAG 4 cut(s) 930, 2200, 2832, 2894
BfoI RGCGCY 2 cut(s) 2585, 2770
BfuAI ACCTGC 4 cut(s) 1285, 2092, 3568, 3964
BfuI GTATCC 1 cut(s) 2722
BlpI GCTNAGC 2 cut(s) 437, 840
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
Bme18I GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2890, 3165
BmgBI CACGTC 1 cut(s) 2091
BmgT120I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2890, 3165
BmrFI CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
BmrI ACTGGG 2 cut(s) 2227, 3042
BmuI ACTGGG 2 cut(s) 2227, 3042
BpiI GAAGAC 4 cut(s) 80, 528, 3234, 3573
BpmI CTGGAG 3 cut(s) 245, 790, 942
Bpu10I CCTNAGC 3 cut(s) 510, 804, 3443
Bpu1102I GCTNAGC 2 cut(s) 437, 840
Bpu14I TTCGAA 3 cut(s) 341, 2851, 2971
BpuEI CTTGAG 7 cut(s) 161, 975, 1060, 1769, 3346, 3707, 3809
Bsa29I ATCGAT 1 cut(s) 3896
BsaAI YACGTR 1 cut(s) 857
BsaBI GATNNNNATC 1 cut(s) 2052
BsaHI GRCGYC 2 cut(s) 2767, 3087
BsaI GGTCTC 2 cut(s) 2110, 3675
BsaWI WCCGGW 1 cut(s) 1125
BsaXI ACNNNNNCTCC 4 cut(s) 2050, 2080, 3394, 3424
Bse118I RCCGGY 1 cut(s) 2082
Bse1I ACTGG 9 cut(s) 7, 959, 1266, 1330, 1437, 2222, 2730, 2743, 3048
Bse3DI GCAATG 4 cut(s) 2620, 3338, 3789, 3885
Bse8I GATNNNNATC 1 cut(s) 2052
BseBI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
BseCI ATCGAT 1 cut(s) 3896
BseJI GATNNNNATC 1 cut(s) 2052
BseMI GCAATG 4 cut(s) 2620, 3338, 3789, 3885
BseNI ACTGG 9 cut(s) 7, 959, 1266, 1330, 1437, 2222, 2730, 2743, 3048
BseRI GAGGAG 4 cut(s) 651, 792, 1993, 3008
BseSI GKGCMC 3 cut(s) 562, 971, 2217
BseX3I CGGCCG 1 cut(s) 31
BseYI CCCAGC 2 cut(s) 2279, 3349
BsgI GTGCAG 1 cut(s) 3845
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 9 cut(s) 26, 33, 399, 560, 745, 948, 1243, 1953, 2456
BshNI GGYRCC 2 cut(s) 2493, 2766
BshVI ATCGAT 1 cut(s) 3896
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 2 cut(s) 1126, 2083
BslFI GGGAC 2 cut(s) 323, 2983
BsmAI GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3331, 3675
BsmFI GGGAC 2 cut(s) 323, 2983
BsmI GAATGC 2 cut(s) 1738, 2352
BsnI GGCC 9 cut(s) 26, 33, 399, 560, 745, 948, 1243, 1953, 2456
Bso31I GGTCTC 2 cut(s) 2110, 3675
Bsp119I TTCGAA 3 cut(s) 341, 2851, 2971
Bsp120I GGGCCC 1 cut(s) 558
Bsp1286I GDGCHC 4 cut(s) 47, 562, 971, 2217
Bsp1407I TGTACA 2 cut(s) 54, 2773
Bsp1720I GCTNAGC 2 cut(s) 437, 840
Bsp19I CCATGG 1 cut(s) 949
BspACI CCGC 5 cut(s) 240, 590, 1482, 1769, 4000
BspANI GGCC 9 cut(s) 26, 33, 399, 560, 745, 948, 1243, 1953, 2456
BspDI ATCGAT 1 cut(s) 3896
BspHI TCATGA 3 cut(s) 295, 1906, 3034
BspMAI CTGCAG 1 cut(s) 2898
BspMI ACCTGC 4 cut(s) 1285, 2092, 3568, 3964
BspQI GCTCTTC 1 cut(s) 113
BspT104I TTCGAA 3 cut(s) 341, 2851, 2971
BspT107I GGYRCC 2 cut(s) 2493, 2766
BspTNI GGTCTC 2 cut(s) 2110, 3675
BsrDI GCAATG 4 cut(s) 2620, 3338, 3789, 3885
BsrFI RCCGGY 1 cut(s) 2082
BsrGI TGTACA 2 cut(s) 54, 2773
BsrI ACTGG 9 cut(s) 7, 959, 1266, 1330, 1437, 2222, 2730, 2743, 3048
BssAI RCCGGY 1 cut(s) 2082
BssNI GRCGYC 2 cut(s) 2767, 3087
BssT1I CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2124, 3734
Bst2UI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
Bst4CI ACNGT 5 cut(s) 52, 359, 741, 2628, 2795
Bst6I CTCTTC 4 cut(s) 113, 279, 1302, 2970
BstACI GRCGYC 2 cut(s) 2767, 3087
BstAPI GCANNNNNTGC 3 cut(s) 1096, 2500, 3832
BstAUI TGTACA 2 cut(s) 54, 2773
BstBAI YACGTR 1 cut(s) 857
BstBI TTCGAA 3 cut(s) 341, 2851, 2971
BstC8I GCNNGC 6 cut(s) 125, 810, 1632, 2492, 2646, 3637
BstDSI CCRYGG 2 cut(s) 34, 949
BstENI CCTNNNNNAGG 1 cut(s) 1523
BstH2I RGCGCY 2 cut(s) 2585, 2770
BstHHI GCGC 3 cut(s) 2584, 2769, 3801
BstMAI GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3331, 3675
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 8 cut(s) 1096, 1631, 1775, 2500, 3271, 3695, 3832, 3971
BstNI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
BstNSI RCATGY 4 cut(s) 1386, 3842, 3878, 4018
BstSCI CCNGG 9 cut(s) 264, 667, 973, 1049, 2238, 2618, 2716, 3089, 3393
BstSFI CTRYAG 4 cut(s) 930, 2200, 2832, 2894
BstSLI GKGCMC 3 cut(s) 562, 971, 2217
BstSNI TACGTA 1 cut(s) 857
BstV2I GAAGAC 4 cut(s) 80, 528, 3234, 3573
BstX2I RGATCY 5 cut(s) 649, 721, 1033, 1478, 3235
BstXI CCANNNNNNTGG 1 cut(s) 1843
BstYI RGATCY 5 cut(s) 649, 721, 1033, 1478, 3235
BstZI CGGCCG 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 3896
BsuI GTATCC 1 cut(s) 2722
BsuRI GGCC 9 cut(s) 26, 33, 399, 560, 745, 948, 1243, 1953, 2456
BsuTUI ATCGAT 1 cut(s) 3896
BtgI CCRYGG 2 cut(s) 34, 949
BtgZI GCGATG 1 cut(s) 1962
BtrI CACGTC 1 cut(s) 2091
BtsI GCAGTG 4 cut(s) 1438, 1625, 2681, 3966
BveI ACCTGC 4 cut(s) 1285, 2092, 3568, 3964
Cac8I GCNNGC 6 cut(s) 125, 810, 1632, 2492, 2646, 3637
CaiI CAGNNNCTG 2 cut(s) 2893, 3560
CciI TCATGA 3 cut(s) 295, 1906, 3034
CfoI GCGC 3 cut(s) 2584, 2769, 3801
Cfr10I RCCGGY 1 cut(s) 2082
Cfr13I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2890, 3165
ClaI ATCGAT 1 cut(s) 3896
CseI GACGC 2 cut(s) 91, 3095
CsiI ACCWGGT 1 cut(s) 2716
DinI GGCGCC 1 cut(s) 2768
DraI TTTAAA 1 cut(s) 2382
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 3 cut(s) 31, 397, 2454
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 4 cut(s) 113, 279, 1302, 2970
EarI CTCTTC 4 cut(s) 113, 279, 1302, 2970
EclXI CGGCCG 1 cut(s) 31
Eco105I TACGTA 1 cut(s) 857
Eco130I CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2124, 3734
Eco24I GRGCYC 1 cut(s) 562
Eco31I GGTCTC 2 cut(s) 2110, 3675
Eco32I GATATC 2 cut(s) 3622, 3894
Eco47I GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2890, 3165
Eco47III AGCGCT 1 cut(s) 2583
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 6 cut(s) 116, 303, 1233, 3171, 3338, 3684
EcoNI CCTNNNNNAGG 1 cut(s) 1523
EcoO109I RGGNCCY 3 cut(s) 310, 559, 2890
EcoRI GAATTC 2 cut(s) 925, 2973
EcoRII CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2618, 2716, 3089, 3393
EcoRV GATATC 2 cut(s) 3622, 3894
EcoT14I CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2124, 3734
EcoT22I ATGCAT 3 cut(s) 547, 2179, 2689
EcoT38I GRGCYC 1 cut(s) 562
EgeI GGCGCC 1 cut(s) 2768
EheI GGCGCC 1 cut(s) 2768
ErhI CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2124, 3734
FalI AAGNNNNNCTT 6 cut(s) 587, 619, 1079, 1111, 2845, 2877
FaqI GGGAC 2 cut(s) 323, 2983
FauI CCCGC 2 cut(s) 233, 583
FauNDI CATATG 2 cut(s) 541, 2823
FbaI TGATCA 3 cut(s) 1387, 2884, 3598
FblI GTMKAC 2 cut(s) 1139, 3754
FriOI GRGCYC 1 cut(s) 562
FspBI CTAG 9 cut(s) 158, 429, 629, 678, 788, 827, 1133, 2349, 3656
GlaI GCGC 3 cut(s) 2583, 2768, 3800
GsaI CCCAGC 2 cut(s) 2283, 3353
GsuI CTGGAG 3 cut(s) 245, 790, 942
HaeII RGCGCY 2 cut(s) 2585, 2770
HaeIII GGCC 9 cut(s) 26, 33, 399, 560, 745, 948, 1243, 1953, 2456
HapII CCGG 2 cut(s) 1126, 2083
HgaI GACGC 2 cut(s) 91, 3095
HhaI GCGC 3 cut(s) 2584, 2769, 3801
Hin1I GRCGYC 2 cut(s) 2767, 3087
Hin6I GCGC 3 cut(s) 2582, 2767, 3799
HinP1I GCGC 3 cut(s) 2582, 2767, 3799
HincII GTYRAC 2 cut(s) 1489, 1758
HindII GTYRAC 2 cut(s) 1489, 1758
HindIII AAGCTT 3 cut(s) 125, 1254, 1632
HpaI GTTAAC 1 cut(s) 1758
HpaII CCGG 2 cut(s) 1126, 2083
HphI GGTGA 9 cut(s) 875, 1389, 2015, 2753, 2890, 3095, 3535, 3584, 3608
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 7 cut(s) 337, 1047, 1463, 1617, 2757, 3225, 3734
HpyCH4III ACNGT 5 cut(s) 52, 359, 741, 2628, 2795
HpyCH4IV ACGT 4 cut(s) 484, 856, 2090, 3517
HpyF10VI GCNNNNNNNGC 8 cut(s) 1096, 1631, 1775, 2500, 3271, 3695, 3832, 3971
HpySE526I ACGT 4 cut(s) 484, 856, 2090, 3517
Hsp92I GRCGYC 2 cut(s) 2767, 3087
HspAI GCGC 3 cut(s) 2582, 2767, 3799
KasI GGCGCC 1 cut(s) 2766
Ksp22I TGATCA 3 cut(s) 1387, 2884, 3598
KspAI GTTAAC 1 cut(s) 1758
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 7 cut(s) 511, 905, 1291, 2422, 2832, 3415, 3736
MabI ACCWGGT 1 cut(s) 2716
MaeI CTAG 9 cut(s) 158, 429, 629, 678, 788, 827, 1133, 2349, 3656
MaeII ACGT 4 cut(s) 484, 856, 2090, 3517
MaeIII GTNAC 8 cut(s) 2468, 2622, 2741, 2986, 3083, 3429, 3775, 3870
MfeI CAATTG 1 cut(s) 1664
MflI RGATCY 5 cut(s) 649, 721, 1033, 1478, 3235
MhlI GDGCHC 4 cut(s) 47, 562, 971, 2217
MlsI TGGCCA 1 cut(s) 2456
MluNI TGGCCA 1 cut(s) 2456
Mly113I GGCGCC 1 cut(s) 2767
MlyI GAGTC 6 cut(s) 296, 868, 1065, 2072, 3005, 3800
MmeI TCCRAC 3 cut(s) 366, 962, 2023
Mox20I TGGCCA 1 cut(s) 2456
Mph1103I ATGCAT 3 cut(s) 547, 2179, 2689
MscI TGGCCA 1 cut(s) 2456
MslI CAYNNNNRTG 4 cut(s) 540, 1184, 2456, 2822
Msp20I TGGCCA 1 cut(s) 2456
MspA1I CMGCKG 3 cut(s) 728, 3442, 4000
MspI CCGG 2 cut(s) 1126, 2083
MspR9I CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
MunI CAATTG 1 cut(s) 1664
Mva1269I GAATGC 2 cut(s) 1738, 2352
MvaI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
MwoI GCNNNNNNNGC 8 cut(s) 1096, 1631, 1775, 2500, 3271, 3695, 3832, 3971
NarI GGCGCC 1 cut(s) 2767
NcoI CCATGG 1 cut(s) 949
NdeI CATATG 2 cut(s) 541, 2823
NmuCI GTSAC 3 cut(s) 2468, 2741, 3083
NsiI ATGCAT 3 cut(s) 547, 2179, 2689
NspI RCATGY 4 cut(s) 1386, 3842, 3878, 4018
NspV TTCGAA 3 cut(s) 341, 2851, 2971
PagI TCATGA 3 cut(s) 295, 1906, 3034
PciSI GCTCTTC 1 cut(s) 113
PctI GAATGC 2 cut(s) 1738, 2352
PflMI CCANNNNNTGG 1 cut(s) 3491
PleI GAGTC 6 cut(s) 295, 868, 1065, 2071, 3004, 3799
PluTI GGCGCC 1 cut(s) 2770
PpsI GAGTC 6 cut(s) 295, 868, 1065, 2071, 3004, 3799
Ppu21I YACGTR 1 cut(s) 857
PpuMI RGGWCCY 2 cut(s) 310, 2890
PshBI ATTAAT 2 cut(s) 1680, 2250
PsiI TTATAA 1 cut(s) 3285
Psp5II RGGWCCY 2 cut(s) 310, 2890
Psp6I CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2618, 2716, 3089, 3393
PspFI CCCAGC 2 cut(s) 2279, 3349
PspGI CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2618, 2716, 3089, 3393
PspOMI GGGCCC 1 cut(s) 558
PspPI GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2890, 3165
PspPPI RGGWCCY 2 cut(s) 310, 2890
PstI CTGCAG 1 cut(s) 2898
PstNI CAGNNNCTG 2 cut(s) 2893, 3560
PsuI RGATCY 5 cut(s) 649, 721, 1033, 1478, 3235
PvuII CAGCTG 2 cut(s) 728, 3442
RseI CAYNNNNRTG 4 cut(s) 540, 1184, 2456, 2822
SapI GCTCTTC 1 cut(s) 113
Sau96I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2890, 3165
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 6 cut(s) 296, 868, 1065, 2072, 3005, 3800
ScrFI CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2620, 2718, 3091, 3395
SduI GDGCHC 4 cut(s) 47, 562, 971, 2217
SexAI ACCWGGT 1 cut(s) 2716
SfcI CTRYAG 4 cut(s) 930, 2200, 2832, 2894
SfoI GGCGCC 1 cut(s) 2768
SfuI TTCGAA 3 cut(s) 341, 2851, 2971
SinI GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2890, 3165
SmiMI CAYNNNNRTG 4 cut(s) 540, 1184, 2456, 2822
SmlI CTYRAG 7 cut(s) 176, 990, 1075, 1748, 3361, 3722, 3788
SmoI CTYRAG 7 cut(s) 176, 990, 1075, 1748, 3361, 3722, 3788
SnaBI TACGTA 1 cut(s) 857
SsiI CCGC 5 cut(s) 240, 590, 1482, 1769, 4000
SspDI GGCGCC 1 cut(s) 2766
SspI AATATT 4 cut(s) 172, 1408, 2524, 3643
SspMI CTAG 9 cut(s) 158, 429, 629, 678, 788, 827, 1133, 2349, 3656
StyD4I CCNGG 9 cut(s) 264, 667, 973, 1049, 2238, 2618, 2716, 3089, 3393
StyI CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2124, 3734
TaaI ACNGT 5 cut(s) 52, 359, 741, 2628, 2795
TaiI ACGT 4 cut(s) 487, 859, 2093, 3520
TatI WGTACW 4 cut(s) 54, 84, 571, 2773
TauI GCSGC 1 cut(s) 4003
TseFI GTSAC 3 cut(s) 2468, 2741, 3083
Tsp45I GTSAC 3 cut(s) 2468, 2741, 3083
TspGWI ACGGA 1 cut(s) 2315
Van91I CCANNNNNTGG 1 cut(s) 3491
VpaK11BI GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2890, 3165
VspI ATTAAT 2 cut(s) 1680, 2250
XagI CCTNNNNNAGG 1 cut(s) 1523
XapI RAATTY 8 cut(s) 244, 617, 703, 925, 1921, 1929, 2908, 2973
XceI RCATGY 4 cut(s) 1386, 3842, 3878, 4018
XmiI GTMKAC 2 cut(s) 1139, 3754
XspI CTAG 9 cut(s) 158, 429, 629, 678, 788, 827, 1133, 2349, 3656
ZrmI AGTACT 1 cut(s) 86
Zsp2I ATGCAT 3 cut(s) 547, 2179, 2689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.