MD16G1176300.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011033
Physical Location & Seq
Reverse (-)
14966529 .. 14974453
7925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1176300.v1.1.491

Sequence Viewer

Length: 4098 bp
ATGAACCAGTACCACGTCTACGAAGCCATTGGCCGCGGAAAATGCTCGACTGTGTACAAAGGGAGGAAAAAGAAGACCATCGAGTACTTCGCGATTAAGAGCGTCGAGAAATCGCAGAAGAGCAAGCTTCTGCAAGAAGTGAAGATTCTTCACACTCTAGATCATCAAAATATCCTCAAGTTTTACTGGTGGTATGAAACTTCTGCTCACTTGTGGTTGGTTTTGGAGTATTGCGTTGGGGGCAATTTGATGACACTATTAAACCAGGATAAGCAGCTACCTGAAGAGTCAATTCATGACCTTGGGAGTGACCTTGTTAGAGCTTTGCAGTTTTTACACTCAAAGGGAATCATATACTGTGACTTGAAACCATCAAATATCCTATTGGATGAGAATGGCCGTACAAAGCTATGTGATTTTGGATTGGCGAGAAAATTGAGCGACATATCACAAATCCCTTCTTCTTCTTTGCCGCAAGCAAAACGCGGAACACCTTGTTATATGGCTCCTGAGCTGTTTGAAGAAGGGGGTGTTCATTCATATGCATCTGACTTATGGGCCCTTGGTTGTGTACTGTATGAGTGCTATGCTGGGAGGCCTCCTTTTGTTGGTAGGGAGTTTACTCTGCTGGTAAAATCCATTATCTCAGATCCAACACCGCCTCTACCTGGCACTCCTAGCCGTCCTTTTGTCAATCTCATAAATTCTTTACTCATAAAGGATCCGGCAGAAAGAATACAGTGGCTTGAACTTTGTGGTCATGCTTTCTGGAGGACTAAGTTAACTGCAGTGCCTCTGCCTCCTCAGCCTGCTTTTGATAATATGCTAGAGCAATATGCTAAGCCATGTCTGTCAGAGCGCAATGGGGATAAATCTTCCCAAATCAGAACTCCTAAATGTCGCCAAAATGATGTTAAAGGAACTTTTAAGCAGGATGAGAATTCTATTTTAGGAGCAAAAGGTGTTGAGACTCCAGTAAAGGGGACACCTGGGGGCCGCAGAACTCAAGCAAAGGTTTCTGGCAGAGGGGTTGATGAAAACCAAAAGGGTCCTTCTTGTGCCACTAGGGGTGTGAATCTTTTGAGGCTCTCAAGAATCGCAAAACAGAACCTGCAGAGGGAAAATGAGAAGGAAAACTACCGCAGGCCGGTGTCTACTGATACTGAGAATGATTCTGAAGTCAAAATTGAGAACACGGATATGGAACTTGATTTTAATGAGAACGCGGAAGAAGACACACAGGATGAATCTGATGGGTCCGATATCCAAGGTTGTACACCTGATAACAAATTTTCAAGTCAGCACCAGGGTAAAATGGAAGAAATGGAAAACAACTTGAACCATTCAGATGCCCTGCCTGTGGTTAGTACACCTGCCTCAGATGAATCCAGAGTTCATAATCAGGAACCATCTCCCCAGAATATTGAAATGGCTACAATTCCACCCAGTGCCAGTCCTCAAATTAAAAATCAGAGATTTAAAGAAGGTTCAGGATTGGCAGTTGACTATGATTCTTCAAAATCTCCGAATAACCTTTCACAAGTTCTTTGGCATCCAACAGATCTCTCAGTCAGACCCGTGATGCCCACCAGAAAATCTGATAAAAACTCGGAGGTAATTCCTTCTCTTCCCTTTGAGGCACTGCAAGCCTCTGACTTTGTGAAGATCTCTAAAGAGCAGTTGGATGCACTCAGTAGTAAGATTATAGCCATTTTTAATGGGAACAGTAGCATTGGTGAGAAGCAAAATGTAATTAGATACCTTGAGATGTTAAGCAATAATGCTGATGCTGCAAATATCTTGACCAATGGACCTATCATGCTTCTGCTGGTTAAAATGCTCCGGCTGTCCAAGGCTTTGGCTTTACGTGTCCAACTTGCTTCACTGGTTGGTTTGTTGATTCGACATTCTACTTTTATTCAAGATGAATTGGCAGATTCAGGAATTTTGGGTTCACTTGCTGATGGCCTTAGAGACAAGCAGGAAAAGGTGAGGAGGTTTTCTATGGCTGCTTTGGGTGAGTTACTATTCTATATATCCACCCAAATTGATCATGCAGACAACAATCCAATAGAGTCTCCATCAAAGGAAAGCCGGTCCACATCTGGGTGGCAGGTGTCAAATGCATTGCTTTCTTTGGTTTCATCAATTTTACGAAAAGGAGAGGACGATCTAATTCAATTATATGCATTGAGGACGATAGAAAATATTTGCAGTCAAGCAGGGCCCTGGGCAGCTCGTTTGACTAGCCAGGACATGATTAATAACCTATGCTATTTATACAGGGCTGCTGGAAAACAGGAGAGCATGAGGCTCACAGCAGGATCATGCTTGGTCCGTCTGGTTCGTTTTAATCCACCTAGCATTCAACCAGTAATAGAGAAGCTCTCTTTAAAGGAAATAGCATCAGCACTTGTGAAGGGCAGTCTGCGTGAACAGCAAATAAGCTTAAACCTCTTAAACATGGCCATGCTTGGAAGTCATATGTTAACGAATGTTGGCAGGTATCTCCTCCCACTGATGGAGGATAAGAATCTTGTCCCAAGTTTAGTGTCTCTTATTGATCAGGGAAGTGAGGTTTTGAAGGGAAAGGCACTTGTTTTCGTTGCTCTTCTTTGTAAGAGCAGTAGGAGGTGGCTGCCGCATTTTTTTTGCCATGCCAAGTTGCTTTCTGTGGTGGACAGGCTGGTGAAAGAGAAGGACCATTATGTGCAGCAGTGTTTAGAGACATCTTTGTGTGTTATGGCGTCTACTATACCCAGTTTACTGGATACGATTACTGGAGATATTCAGAAAATGATGGGAGGCAGGCGCCATGGGTATCAATCTCCCCTTAACGGTAGAGCTGCTCCAAAGACCAATATTTATATGTTTCCTGTAGTTCTCCATCTTCTTCGGAGCTTGTCTTTTAAGCGCAAGGTGGTGAGTGAACAGGTCCTGCAGCAGTTGGCAAATCTAATGAAACTCGTGGAGACACCATTTCAGGGCAGGGATGACTTCCAGATTACCCTTCTTCGAATTCTTGAGTCTGTCTCAGAGGAGTCACATGTAATTCTTGAAAGCCCTGACGTTTTTATTCATGAAGTTCTCCCCAGTTTGGCTGTTCTTTATAAGGGTAACAAGGATGGTGATGCCAGGTTTCTGTGCTTGAAAATTTTGTTTGACGTGATGGTAATTTTTCTGAATGAAGAGTCTGAAGATCAGCAAAGGTCCAATGAACTGGAATCCATATCCAATAAGAATTTTCTTCCTCTTTTCCCGTCCTTAATTGAAGATGAAGATCCGATTCCCTTGTACGCACAAAAGCTTCTTGTGATGCTTATTGAACTTAATTACATCAAAATTGCAGACATTCTAGATCTTAAGATAGTCTCGCAATGCTTTGAGTTTTTGCTCGGTGATCTCTCAAGTGCAAATGTGAATAATGTCATGCTATGTCTGGCTCTGACATCGGCACCTGAAATGGAAACCAAGTTGCTCTCTCAACTGAAGGTAGTTCGGAAAACAGGAAACCTTCTAGAGTTTGTGTATGCAAAAGATATGGAAGATTTTCTGGAACCAACCCTTGGCTTATGCAGGGCTTTCCTTCTACGGTCAGTGAGTGGTAGAAAAGGCTTCATCTATTCAAAAGAACCTGCTCTTCTAGGTGATGCATCTTCCGAGGCAAGCGGTGCTGATCAGCAACAAGGCATTAGAGATATCATGGACTTCGGCAGCAATGTTGGCGTCTTACTAGAGTTGAGTAGATCCCACGGAGTTAATGTGGCAGATCTAGCTTCAGAATGCGTTGTCTTGTTGCTCAAGGCAGCTCCAAGGGAAGCCACTGCTGGTCTGCTAACTAATCTTCCAAAAATTACTGCAGTCCTCGAGTCTTGGCGTAGGGGCACATCTCACCTATTGGTGCAGCGGGTGCTGCATGCCCTCGGTTATTCTTGTAGGCAATATTTGTTACACGCAATGATACTGTCGATATCTATACCCGAGATCTCAAGAATTGAAGGCATTGTTTCTGAGCTTAAAGGTTCAGGTGTACCTGTTTTAGCCACTGCTGCTTTCCATGTGGCTGTGGAGTTGCAACGGCTGCCTCGCTGCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1366

Amino Acids

151.96

Weight (kDa)

6.5

Isoelectric Point (pI)

49.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 256 2.2e-64 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 251 1.7e-37 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 66 - 148 4.6e-07 ABC1 atypical kinase-like domain
HEAT_ULK4 PF23606 922 - 1115 1e-73 Serine/threonine-protein kinase ULK4-like, HEAT repeats
ARM_RUK PF24970 1122 - 1364 5.8e-122 RUNKEL ARM-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3111
AarI CACCTGC 2 cut(s) 1381, 2104
Acc36I ACCTGC 5 cut(s) 1119, 1381, 2104, 2493, 3643
AccB1I GGYRCC 2 cut(s) 2811, 3454
AccB7I CCANNNNNTGG 1 cut(s) 3566
AccI GTMKAC 3 cut(s) 18, 1154, 2750
AccII CGCG 4 cut(s) 36, 92, 486, 1226
AclWI GGATC 6 cut(s) 644, 716, 729, 2332, 3275, 3741
AcoI YGGCCR 3 cut(s) 31, 397, 2466
AcsI RAATTY 7 cut(s) 703, 940, 1289, 1944, 3018, 3153, 3241
AcuI CTGAAG 5 cut(s) 303, 1197, 3216, 3509, 3762
AcyI GRCGYC 3 cut(s) 2747, 2812, 3726
AdeI CACNNNGTG 1 cut(s) 1448
AfaI GTAC 9 cut(s) 11, 56, 86, 403, 573, 1276, 1369, 3296, 4032
AflII CTTAAG 1 cut(s) 3362
AflIII ACRYGT 2 cut(s) 1867, 3046
AjiI CACGTC 2 cut(s) 16, 3166
AjnI CCWGG 7 cut(s) 264, 667, 988, 1305, 2228, 2250, 3134
AjuI GAANNNNNNNTTGG 2 cut(s) 3549, 3581
Alw26I GTCTC 8 cut(s) 962, 1968, 2082, 2559, 2720, 2966, 3037, 3376
AlwI GGATC 6 cut(s) 644, 716, 729, 2332, 3275, 3741
AlwNI CAGNNNCTG 2 cut(s) 1111, 2938
Ama87I CYCGRG 2 cut(s) 3866, 3980
AoxI GGCC 9 cut(s) 31, 397, 558, 596, 994, 1145, 1966, 2225, 2466
ApaI GGGCCC 2 cut(s) 562, 2229
ApoI RAATTY 7 cut(s) 703, 940, 1289, 1944, 3018, 3153, 3241
AseI ATTAAT 1 cut(s) 2262
Asp700I GAANNNNTTC 1 cut(s) 3549
AspLEI GCGC 3 cut(s) 861, 2814, 2916
AsuHPI GGTGA 9 cut(s) 1748, 2002, 2030, 2701, 2935, 3140, 3410, 3659, 3884
AsuII TTCGAA 1 cut(s) 3016
AvaI CYCGRG 2 cut(s) 3866, 3980
AvaII GGWCC 8 cut(s) 1049, 1257, 1811, 2097, 2335, 2701, 2935, 3210
BaeGI GKGCMC 3 cut(s) 562, 2229, 3887
BalI TGGCCA 1 cut(s) 2468
BamHI GGATCC 1 cut(s) 721
BanI GGYRCC 2 cut(s) 2811, 3454
BanII GRGCYC 2 cut(s) 562, 2229
BarI GAAGNNNNNNTAC 2 cut(s) 1121, 1153
BauI CACGAG 1 cut(s) 2966
BbsI GAAGAC 2 cut(s) 80, 1239
BbvCI CCTCAGC 1 cut(s) 804
BceAI ACGGC 3 cut(s) 384, 666, 4094
BciT130I CCWGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
BciVI GTATCC 1 cut(s) 2764
BclI TGATCA 3 cut(s) 2050, 2563, 3676
BcoDI GTCTC 8 cut(s) 962, 1968, 2082, 2559, 2720, 2966, 3037, 3376
BfmI CTRYAG 5 cut(s) 786, 1112, 2877, 2939, 3858
BfoI RGCGCY 1 cut(s) 2815
BfrI CTTAAG 1 cut(s) 3362
BfuAI ACCTGC 5 cut(s) 1119, 1381, 2104, 2493, 3643
BfuI GTATCC 1 cut(s) 2764
BglII AGATCT 5 cut(s) 1561, 1665, 3358, 3769, 3984
BlpI GCTNAGC 1 cut(s) 840
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
Bme18I GGWCC 8 cut(s) 1049, 1257, 1811, 2097, 2335, 2701, 2935, 3210
BmeT110I CYCGRG 2 cut(s) 3866, 3980
BmgBI CACGTC 2 cut(s) 16, 3166
BmrFI CCNGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
BmrI ACTGGG 3 cut(s) 1440, 2754, 3087
BmuI ACTGGG 3 cut(s) 1440, 2754, 3087
BpiI GAAGAC 2 cut(s) 80, 1239
BplI GAGNNNNNCTC 4 cut(s) 2372, 2404, 3017, 3049
BpmI CTGGAG 3 cut(s) 790, 957, 2802
Bpu10I CCTNAGC 2 cut(s) 510, 804
Bpu1102I GCTNAGC 1 cut(s) 840
Bpu14I TTCGAA 1 cut(s) 3016
BpuEI CTTGAG 8 cut(s) 161, 990, 1075, 1784, 3044, 3391, 3785, 3973
BsaAI YACGTR 1 cut(s) 1868
BsaBI GATNNNNATC 2 cut(s) 2532, 3279
BsaHI GRCGYC 3 cut(s) 2747, 2812, 3726
BsaXI ACNNNNNCTCC 2 cut(s) 3747, 3777
Bse118I RCCGGY 2 cut(s) 1147, 2094
Bse3DI GCAATG 5 cut(s) 868, 2126, 3383, 3724, 3963
Bse8I GATNNNNATC 2 cut(s) 2532, 3279
BseBI CCWGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
BseGI GGATG 7 cut(s) 394, 940, 1249, 1552, 1690, 2998, 3130
BseJI GATNNNNATC 2 cut(s) 2532, 3279
BseMI GCAATG 5 cut(s) 868, 2126, 3383, 3724, 3963
BseMII CTCAG 9 cut(s) 501, 660, 818, 1155, 1392, 1581, 1705, 3048, 4002
BseRI GAGGAG 4 cut(s) 792, 2008, 2501, 3053
BseSI GKGCMC 3 cut(s) 562, 2229, 3887
BseYI CCCAGC 1 cut(s) 590
BsgI GTGCAG 2 cut(s) 2732, 3923
Bsh1236I CGCG 4 cut(s) 36, 92, 486, 1226
BshFI GGCC 9 cut(s) 33, 399, 560, 598, 996, 1147, 1968, 2227, 2468
BshNI GGYRCC 2 cut(s) 2811, 3454
BsiHKCI CYCGRG 2 cut(s) 3866, 3980
BsiSI CCGG 4 cut(s) 725, 1148, 1843, 2095
BslFI GGGAC 2 cut(s) 997, 2525
BsmAI GTCTC 8 cut(s) 962, 1968, 2082, 2559, 2720, 2966, 3037, 3376
BsmFI GGGAC 2 cut(s) 997, 2525
BsmI GAATGC 2 cut(s) 2364, 3788
BsnI GGCC 9 cut(s) 33, 399, 560, 598, 996, 1147, 1968, 2227, 2468
BsoBI CYCGRG 2 cut(s) 3866, 3980
Bsp119I TTCGAA 1 cut(s) 3016
Bsp120I GGGCCC 2 cut(s) 558, 2225
Bsp1286I GDGCHC 3 cut(s) 562, 2229, 3887
Bsp1407I TGTACA 2 cut(s) 54, 1274
Bsp1720I GCTNAGC 1 cut(s) 840
Bsp19I CCATGG 1 cut(s) 2815
Bsp68I TCGCGA 1 cut(s) 92
BspANI GGCC 9 cut(s) 33, 399, 560, 598, 996, 1147, 1968, 2227, 2468
BspCNI CTCAG 9 cut(s) 502, 659, 817, 1156, 1391, 1580, 1704, 3047, 4003
BspFNI CGCG 4 cut(s) 36, 92, 486, 1226
BspHI TCATGA 2 cut(s) 295, 3079
BspMAI CTGCAG 4 cut(s) 790, 1116, 2943, 3862
BspMI ACCTGC 5 cut(s) 1119, 1381, 2104, 2493, 3643
BspPI GGATC 6 cut(s) 644, 716, 729, 2332, 3275, 3741
BspQI GCTCTTC 3 cut(s) 113, 2616, 3645
BspT104I TTCGAA 1 cut(s) 3016
BspT107I GGYRCC 2 cut(s) 2811, 3454
BspTI CTTAAG 1 cut(s) 3362
BsrDI GCAATG 5 cut(s) 868, 2126, 3383, 3724, 3963
BsrFI RCCGGY 2 cut(s) 1147, 2094
BsrGI TGTACA 2 cut(s) 54, 1274
BssAI RCCGGY 2 cut(s) 1147, 2094
BssNI GRCGYC 3 cut(s) 2747, 2812, 3726
BssSI CACGAG 1 cut(s) 2966
BssT1I CCWWGG 7 cut(s) 301, 562, 1267, 1851, 2815, 3565, 3812
Bst2BI CACGAG 1 cut(s) 2966
Bst2UI CCWGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
Bst4CI ACNGT 8 cut(s) 52, 359, 576, 741, 1727, 2840, 3594, 3966
Bst6I CTCTTC 6 cut(s) 113, 279, 1632, 2616, 3183, 3645
BstACI GRCGYC 3 cut(s) 2747, 2812, 3726
BstAFI CTTAAG 1 cut(s) 3362
BstAPI GCANNNNNTGC 3 cut(s) 3671, 3910, 4081
BstAUI TGTACA 2 cut(s) 54, 1274
BstBAI YACGTR 1 cut(s) 1868
BstBI TTCGAA 1 cut(s) 3016
BstC8I GCNNGC 8 cut(s) 125, 477, 810, 1145, 1647, 2810, 3667, 3918
BstDSI CCRYGG 3 cut(s) 34, 2815, 3751
BstF5I GGATG 7 cut(s) 394, 940, 1249, 1552, 1690, 2998, 3130
BstFNI CGCG 4 cut(s) 36, 92, 486, 1226
BstH2I RGCGCY 1 cut(s) 2815
BstHHI GCGC 3 cut(s) 861, 2814, 2916
BstMAI GTCTC 8 cut(s) 962, 1968, 2082, 2559, 2720, 2966, 3037, 3376
BstNI CCWGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
BstNSI RCATGY 2 cut(s) 3050, 3920
BstSCI CCNGG 7 cut(s) 264, 667, 988, 1305, 2228, 2250, 3134
BstSFI CTRYAG 5 cut(s) 786, 1112, 2877, 2939, 3858
BstSLI GKGCMC 3 cut(s) 562, 2229, 3887
BstUI CGCG 4 cut(s) 36, 92, 486, 1226
BstV2I GAAGAC 2 cut(s) 80, 1239
BstX2I RGATCY 9 cut(s) 649, 721, 1561, 1665, 3280, 3358, 3746, 3769, 3984
BstXI CCANNNNNNTGG 3 cut(s) 1858, 2767, 3220
BstYI RGATCY 9 cut(s) 649, 721, 1561, 1665, 3280, 3358, 3746, 3769, 3984
BsuI GTATCC 1 cut(s) 2764
BsuRI GGCC 9 cut(s) 33, 399, 560, 598, 996, 1147, 1968, 2227, 2468
BtgI CCRYGG 3 cut(s) 34, 2815, 3751
BtrI CACGTC 2 cut(s) 16, 3166
BtsCI GGATG 7 cut(s) 394, 940, 1249, 1552, 1690, 2998, 3130
BtsI GCAGTG 5 cut(s) 795, 1640, 2723, 3822, 4044
BtuMI TCGCGA 1 cut(s) 92
BveI ACCTGC 5 cut(s) 1119, 1381, 2104, 2493, 3643
Cac8I GCNNGC 8 cut(s) 125, 477, 810, 1145, 1647, 2810, 3667, 3918
CaiI CAGNNNCTG 2 cut(s) 1111, 2938
CciI TCATGA 2 cut(s) 295, 3079
CfoI GCGC 3 cut(s) 861, 2814, 2916
Cfr10I RCCGGY 2 cut(s) 1147, 2094
Cfr42I CCGCGG 1 cut(s) 37
CseI GACGC 3 cut(s) 91, 2736, 3715
Csp6I GTAC 9 cut(s) 10, 55, 85, 402, 572, 1275, 1368, 3295, 4031
CspCI CAANNNNNGTGG 2 cut(s) 2029, 2064
CviQI GTAC 9 cut(s) 10, 55, 85, 402, 572, 1275, 1368, 3295, 4031
DinI GGCGCC 1 cut(s) 2813
DraI TTTAAA 2 cut(s) 1480, 2394
DraIII CACNNNGTG 1 cut(s) 1448
EaeI YGGCCR 3 cut(s) 31, 397, 2466
Eam1104I CTCTTC 6 cut(s) 113, 279, 1632, 2616, 3183, 3645
EarI CTCTTC 6 cut(s) 113, 279, 1632, 2616, 3183, 3645
Eco130I CCWWGG 7 cut(s) 301, 562, 1267, 1851, 2815, 3565, 3812
Eco147I AGGCCT 1 cut(s) 598
Eco24I GRGCYC 2 cut(s) 562, 2229
Eco32I GATATC 3 cut(s) 1264, 3700, 3972
Eco47I GGWCC 8 cut(s) 1049, 1257, 1811, 2097, 2335, 2701, 2935, 3210
Eco57I CTGAAG 5 cut(s) 303, 1197, 3216, 3509, 3762
Eco88I CYCGRG 2 cut(s) 3866, 3980
EcoO109I RGGNCCY 5 cut(s) 559, 1049, 2225, 2226, 2935
EcoRI GAATTC 2 cut(s) 940, 3018
EcoRII CCWGG 7 cut(s) 264, 667, 988, 1305, 2228, 2250, 3134
EcoRV GATATC 3 cut(s) 1264, 3700, 3972
EcoT14I CCWWGG 7 cut(s) 301, 562, 1267, 1851, 2815, 3565, 3812
EcoT22I ATGCAT 4 cut(s) 547, 2128, 2191, 3655
EcoT38I GRGCYC 2 cut(s) 562, 2229
EgeI GGCGCC 1 cut(s) 2813
EheI GGCGCC 1 cut(s) 2813
ErhI CCWWGG 7 cut(s) 301, 562, 1267, 1851, 2815, 3565, 3812
FaqI GGGAC 2 cut(s) 997, 2525
FauI CCCGC 1 cut(s) 3900
FauNDI CATATG 2 cut(s) 541, 2484
FbaI TGATCA 3 cut(s) 2050, 2563, 3676
FblI GTMKAC 3 cut(s) 18, 1154, 2750
FokI GGATG 7 cut(s) 401, 947, 1256, 1539, 1697, 3005, 3137
FriOI GRGCYC 2 cut(s) 562, 2229
GlaI GCGC 3 cut(s) 860, 2813, 2915
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 3 cut(s) 790, 957, 2802
HaeII RGCGCY 1 cut(s) 2815
HaeIII GGCC 9 cut(s) 33, 399, 560, 598, 996, 1147, 1968, 2227, 2468
HapII CCGG 4 cut(s) 725, 1148, 1843, 2095
HgaI GACGC 3 cut(s) 91, 2736, 3715
HhaI GCGC 3 cut(s) 861, 2814, 2916
Hin1I GRCGYC 3 cut(s) 2747, 2812, 3726
Hin6I GCGC 3 cut(s) 859, 2812, 2914
HinP1I GCGC 3 cut(s) 859, 2812, 2914
HincII GTYRAC 3 cut(s) 783, 1504, 2490
HindII GTYRAC 3 cut(s) 783, 1504, 2490
HindIII AAGCTT 3 cut(s) 125, 2446, 3305
HpaI GTTAAC 2 cut(s) 783, 2490
HpaII CCGG 4 cut(s) 725, 1148, 1843, 2095
HphI GGTGA 9 cut(s) 1748, 2002, 2030, 2701, 2935, 3140, 3410, 3659, 3884
Hpy99I CGWCG 1 cut(s) 107
HpyCH4III ACNGT 8 cut(s) 52, 359, 576, 741, 1727, 2840, 3594, 3966
HpyCH4IV ACGT 4 cut(s) 15, 1867, 3069, 3165
HpySE526I ACGT 4 cut(s) 15, 1867, 3069, 3165
Hsp92I GRCGYC 3 cut(s) 2747, 2812, 3726
HspAI GCGC 3 cut(s) 859, 2812, 2914
KasI GGCGCC 1 cut(s) 2811
Ksp22I TGATCA 3 cut(s) 2050, 2563, 3676
KspAI GTTAAC 2 cut(s) 783, 2490
KspI CCGCGG 1 cut(s) 37
LguI GCTCTTC 3 cut(s) 113, 2616, 3645
LmnI GCTCC 6 cut(s) 511, 953, 1845, 2854, 2898, 3814
MaeII ACGT 4 cut(s) 15, 1867, 3069, 3165
MaeIII GTNAC 6 cut(s) 308, 359, 2022, 3042, 3116, 3948
MflI RGATCY 9 cut(s) 649, 721, 1561, 1665, 3280, 3358, 3746, 3769, 3984
MhlI GDGCHC 3 cut(s) 562, 2229, 3887
MlsI TGGCCA 1 cut(s) 2468
MluNI TGGCCA 1 cut(s) 2468
Mly113I GGCGCC 1 cut(s) 2812
MlyI GAGTC 7 cut(s) 296, 964, 2084, 3035, 3050, 3200, 3878
MmeI TCCRAC 4 cut(s) 677, 1580, 1662, 1897
Mox20I TGGCCA 1 cut(s) 2468
Mph1103I ATGCAT 4 cut(s) 547, 2128, 2191, 3655
MroXI GAANNNNTTC 1 cut(s) 3549
MscI TGGCCA 1 cut(s) 2468
MslI CAYNNNNRTG 6 cut(s) 540, 1199, 1347, 2107, 2468, 2734
Msp20I TGGCCA 1 cut(s) 2468
MspA1I CMGCKG 2 cut(s) 36, 3907
MspCI CTTAAG 1 cut(s) 3362
MspI CCGG 4 cut(s) 725, 1148, 1843, 2095
MspR9I CCNGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
Mva1269I GAATGC 2 cut(s) 2364, 3788
MvaI CCWGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
MvnI CGCG 4 cut(s) 36, 92, 486, 1226
NarI GGCGCC 1 cut(s) 2812
NcoI CCATGG 1 cut(s) 2815
NdeI CATATG 2 cut(s) 541, 2484
NmuCI GTSAC 3 cut(s) 308, 359, 3042
NruI TCGCGA 1 cut(s) 92
NsiI ATGCAT 4 cut(s) 547, 2128, 2191, 3655
NspI RCATGY 2 cut(s) 3050, 3920
NspV TTCGAA 1 cut(s) 3016
PaeI GCATGC 1 cut(s) 3920
PaeR7I CTCGAG 1 cut(s) 3866
PagI TCATGA 2 cut(s) 295, 3079
PaqCI CACCTGC 2 cut(s) 1381, 2104
PasI CCCWGGG 1 cut(s) 2229
PceI AGGCCT 1 cut(s) 598
PciI ACATGT 1 cut(s) 3046
PciSI GCTCTTC 3 cut(s) 113, 2616, 3645
PctI GAATGC 2 cut(s) 2364, 3788
PdmI GAANNNNTTC 1 cut(s) 3549
PflMI CCANNNNNTGG 1 cut(s) 3566
PleI GAGTC 7 cut(s) 295, 964, 2083, 3034, 3049, 3199, 3877
PluTI GGCGCC 1 cut(s) 2815
PpsI GAGTC 7 cut(s) 295, 964, 2083, 3034, 3049, 3199, 3877
Ppu21I YACGTR 1 cut(s) 1868
PpuMI RGGWCCY 2 cut(s) 1049, 2935
PscI ACATGT 1 cut(s) 3046
PshBI ATTAAT 1 cut(s) 2262
PsiI TTATAA 1 cut(s) 3111
Psp5II RGGWCCY 2 cut(s) 1049, 2935
Psp6I CCWGG 7 cut(s) 264, 667, 988, 1305, 2228, 2250, 3134
PspFI CCCAGC 1 cut(s) 590
PspGI CCWGG 7 cut(s) 264, 667, 988, 1305, 2228, 2250, 3134
PspOMI GGGCCC 2 cut(s) 558, 2225
PspPPI RGGWCCY 2 cut(s) 1049, 2935
PspXI VCTCGAGB 1 cut(s) 3866
PstI CTGCAG 4 cut(s) 790, 1116, 2943, 3862
PstNI CAGNNNCTG 2 cut(s) 1111, 2938
PsuI RGATCY 9 cut(s) 649, 721, 1561, 1665, 3280, 3358, 3746, 3769, 3984
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 9 cut(s) 11, 56, 86, 403, 573, 1276, 1369, 3296, 4032
RsaNI GTAC 9 cut(s) 10, 55, 85, 402, 572, 1275, 1368, 3295, 4031
RseI CAYNNNNRTG 6 cut(s) 540, 1199, 1347, 2107, 2468, 2734
SacII CCGCGG 1 cut(s) 37
SapI GCTCTTC 3 cut(s) 113, 2616, 3645
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 7 cut(s) 296, 964, 2084, 3035, 3050, 3200, 3878
ScrFI CCNGG 7 cut(s) 266, 669, 990, 1307, 2230, 2252, 3136
SduI GDGCHC 3 cut(s) 562, 2229, 3887
SfcI CTRYAG 5 cut(s) 786, 1112, 2877, 2939, 3858
SfoI GGCGCC 1 cut(s) 2813
Sfr274I CTCGAG 1 cut(s) 3866
Sfr303I CCGCGG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 3016
SgrBI CCGCGG 1 cut(s) 37
SinI GGWCC 8 cut(s) 1049, 1257, 1811, 2097, 2335, 2701, 2935, 3210
SlaI CTCGAG 1 cut(s) 3866
SmiMI CAYNNNNRTG 6 cut(s) 540, 1199, 1347, 2107, 2468, 2734
SphI GCATGC 1 cut(s) 3920
SseBI AGGCCT 1 cut(s) 598
SspDI GGCGCC 1 cut(s) 2811
SspI AATATT 4 cut(s) 1423, 2209, 2863, 3944
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 7 cut(s) 264, 667, 988, 1305, 2228, 2250, 3134
StyI CCWWGG 7 cut(s) 301, 562, 1267, 1851, 2815, 3565, 3812
TaaI ACNGT 8 cut(s) 52, 359, 576, 741, 1727, 2840, 3594, 3966
TaiI ACGT 4 cut(s) 18, 1870, 3072, 3168
TaqI TCGA 7 cut(s) 47, 81, 105, 1903, 3016, 3867, 3968
TatI WGTACW 5 cut(s) 54, 84, 571, 1274, 1367
TauI GCSGC 4 cut(s) 36, 475, 999, 2644
TseFI GTSAC 3 cut(s) 308, 359, 3042
Tsp45I GTSAC 3 cut(s) 308, 359, 3042
TspGWI ACGGA 3 cut(s) 1211, 2327, 3768
Van91I CCANNNNNTGG 1 cut(s) 3566
Vha464I CTTAAG 1 cut(s) 3362
VpaK11BI GGWCC 8 cut(s) 1049, 1257, 1811, 2097, 2335, 2701, 2935, 3210
VspI ATTAAT 1 cut(s) 2262
XapI RAATTY 7 cut(s) 703, 940, 1289, 1944, 3018, 3153, 3241
XbaI TCTAGA 3 cut(s) 157, 3355, 3517
XceI RCATGY 2 cut(s) 3050, 3920
XhoI CTCGAG 1 cut(s) 3866
XmiI GTMKAC 3 cut(s) 18, 1154, 2750
XmnI GAANNNNTTC 1 cut(s) 3549
ZrmI AGTACT 1 cut(s) 86
Zsp2I ATGCAT 4 cut(s) 547, 2128, 2191, 3655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.