Rorug04G0166400

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011033
Physical Location & Seq
Reverse (-)
29186139 .. 29190152
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0166400.1

Sequence Viewer

Length: 4014 bp
ATGGTGGTGGAGGTGTTTCTCGGGGCTTTTGTTCAGGTGCTGCTGGAAAGGTTGACACCCCAGAACTTGCTCAGTTTCGGTCACTTCCAAGGCGTCGAAAAGAAGTTCAAGAAATGGAGTACAACTCTGTCTGCAATCCGAGCTGTGCTGCAGGATGCTGAGAAGAAGCAACTCACCAGCGAGGCGGTGAAGCTGTGGCTGGATGAGCTCAAACACTTGGCTTTCGACATGGATGACTTCTTGGACACCTTTTCCACCCAGCTCTTGGTGATCAATCACCAAAATGGGGCCGGCACAAGCAAGGTACAACGCCTCTTTTCGAGAGTTAAATTCAACTTTAACATGAACTCCGAAATGGGTAACATTGCTGACAGATTACAAGTAATATCTGATAGAAAAGATAAGCTCGGTTTAGTATATCGTGATGATCCAGGTTATCCTCGGGCATCACAAAGGTTGCCGAGTTCATATGTGTTGGATGGACCTGTGGTTGGAAGGGATGAAGACAAGAGAAAGATTGTTGAGTTGTTGTTGAGAGATCATGAGCCTTGTTGTACAACCAATTTCCAAGTTTTGGCCATTGTTGGTATGCCGGGACTTGGCAAGACCACACTTGCAGGGCATGTGTTTAATGATAAGGAAACGGGGCTGTTCAATCCAAAGGTGTGGGTATCTGTTTCTGATAGCTTCAGTCTTGAGAGACTGACGAAAACTATCTTCAAGTTAGTCACATCCCGGGATTCTGATGATTTGGATGAGTTTAGTAAAGTGCAAGATCTTCTGAGTGAGGCATTAGCAGGAAAAAAGTTTCTAATTGTTCTGGATGATGTTTGGAGTACTTGTGACTATGATTCTTGGACAAAACTCCAGTCTCCCTTTCGTGTTGGAGCACCAGGAAGTCAGATAGTGGTGACTACTCGTGAAGAAAAAGTTGCGAAATTGATAGGAGCAACTCATGTTTATGATTTGAAGACCCTATCAAATGAGGATTGTTTGGAAGTATTTCAGCAGCATATTAACAGTAATAGACCACCAAATTTTAATCTTCTTTGCAAGAAGATTGTTGAAAAGTGCAATGGATTGCCATTGGCTGCAAAGACTCTTGGTGGCATTTTACGTTGTAAAGAAGCTGGCAGTTGGGAAGAAGTATTGGATGATAAATTGTGGAGTGTGTCAAATAATGAGAGCAACATTCTCACAGTACTAAAATTGAGCTATCATTATCTTCCTTCAAACTTGAAGAGGTGCTTTGCGTATTGCTCAATACTTCCAAAGCAATATGAATTTGGGAAAAAGCAGTTAATACTGCTGTGGATGGCAGAGGGTTTTCTTCATCAATCACAAGGAAGTAAGGAGATGGAAGATATTGGCGGTGACTACTTTGGGGAGCTGTTGTCTCGGTCATTGTTTCAGAAGTCAAGCAAAAATAATTCAAGATATGAAATGCATGACCTTGTTGGTGATTTGGCACGGTGGGCTGCAGGAGATATCTGTTTTAGATTGGAGGATAATCTGGATGGAAGATGTTCTCCAAAGACTCGTTATTCGAGTTACAGTTCTGGCAAGTTTGATGGGGTAAAAAAATTCGAAGCCTTCTCTAAAGCCAAACATTTGCGGACTTTCTTACCAGTTTCAGTTTTACGCGGTCGTGAGAATCATCAAACTCGTAAGGTTGCTTCTGATTTATTGCCAAATATGAAGTACTTACGGGTGCTCTCTTTCAATGGATATCAAATAACTGAACTGCCGGATTCAATTGGCAGACTGAAGCATCTAAGGTACCTTGATCTGTCTCACACCTTGATAATGAGTTTGCCTGATTCAATAAGCTCTCTTTACAACTTACAGACGTTGATATTGGAAAATTGTTCTAAATTGAAGGCACTACCTTCAAACATTAGGAATTTAATTAATCTACGTCATCTCAACAATTCTAGCATGCTTTCTTTGGAAGGAATGCCTCCACAATTAGGTCAACTGACTAGCCTGCAAACGTTATCCTGTTTCGTGGTGGGCAAAGCTAGGGAGTCAGGTGTGAAAGAGATAGGCCCCCTATATCTTCTTCGTGGGACATTGTGCCTCTCACGATTAGAAAATGTCATCGATGTGGAGGATGCAAGAAGGGCTGACTTAATATGCAAGGAAGGGCTTGATGCATTGCAACTAGAATGGAGTGGCAGAGGAGAAAAGGAATCAGATGTGCTTGACATGTTAAAACCCCACAGAAACCTCAAAGAGCTTACCATTAAAGGCTATGGTGGTTTGGAATTGCCATCATGGATATGTAATCCTTTATTCTCTGTTCTGGTGCTTTTAAGGTTAGAGAATTGTAATAATTGTCGATTCTTGCCACAACTCGGACAATTACCTTCTCTCAAGGAACTCTTCATAAGAGGAATGTCTCAGGTGGAAAGTGTTGGTGTTGAGTTTTATGGAGAGGGTACCTTGTCTTTTCCAGTACTGGAGAATCTAGTGTTTGAGGATATGCAACAATGGAAGGGATGGTTTCCTTGCGAAGAAGATCAAATTGGAGTTTTTCCTTCCTTGAAAATGCTTTCTGTCATTAAATGTCCACAATTGGTGGTTTCCATTACAAACTATACACAACTTTGTGCATTACAAGTCCATGACTGCAATGGGGTGGTATTTAGAGGTGCTGTTGACTTTGAGTTACTTGAGCATATGTATCTTTCAAATTTGTCAGTGTTGAGACTTGAAATTGAAAAATCTGCGGAATGCATGAGCGGCTTAAGAAATGTTCAAGGCTTGGGAATTACTAATAGCGAGGAATCATCATCTTCATGGCAGAATGAGGATATAATACTGCAGTATCTGAGTTCTCTTCGTAGTTTGTTTATTGAAAGGAACTCCAAGTTTCTTCAATTGCATCAGCTGACATCACTTCAAGCGCTTCGCTTATATAGATGTGCAAGTCTGGTTTCCATTCCCGAAGCATGTTTGCCACCTTCTCTTAAAGATCTGTGGATTGAAAGGTGCGATTCTCTCACGTATTTTGCAAGGTACCAGATACCTCCAAGTCTAAGAAGAGCAGGAATAAGAGAATGCAAAAGGTTGAAATTATTAGTTGGGGAGAATGTAGAGGGTTACTCATCCTCTTCTTCTTCGCACAGTTTGATGCAAGAGGATACATCTTGTCTTGAGTACTTGGAGGTATGGGAGTGTCCATCTCTTACATCCTTATCATCAAGAGGCCAACTACCCAGAGCGCTTAAACACCTTCACATAAGGGATTGTGAACGACTGGAGTCAATGACAGATATGTTCCACAGTGAAACTTGTCTCGAACATATTGTGATACAGAGTTGTGCAAATCTTAAATCCTTACCAGAAGGCCTATGCGACCTCACGCATCTTCAACAGTTAATTGTTATGGATTGTGGAAGTCTGGTTTCCTTCCCGAGAGGAGGGTTGCCAACAACAGTCTCCAACCTGACAAGCATCTATATCTACAATTGTGATAGCTTGGAAGCTTTCCCCAGAGGAATGGACAAGTACATCTCTCTTCAGACATTATGGATCGGTTACTGTAAAGGTTTGTCATCCATTTTAGAAGAGGGTTTTTCCCCAAACTTAGTTGAGCTTTACATTGTGAACCCTTTACGTTGCAAGCCACTCTGTGAATGGGGGTTGCAGTTGCACAGACTCAATTCTCTGAGAGAGTTGTGGATCCAGGGTGTAGATCCAAATCTGGTGTTATTTCCACCAGAGGAGATGGAGATGCTGCTCCCTAAATCTCTCATTAAAATCAGAATTGGAGACTTCCCAAAGCTAAGGCGCCTATCCAGCAAGGCCCTTCAGTCGCTCACTTCTCTTGAATCTCTTAGAATTGAAGATTGTCCAAAGCTAGCATCCATTCCAGAGGAGAAGCTGTCTCTTTCACTTACACAACTGCATATCTCCAATTGTCCTCTGCTAAAGAAGAGATACAGACCAGGGAAAGCACCACACTGGCCCAAAATAGGCCACATTCCTTACATACAGATTGGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1337

Amino Acids

151.42

Weight (kDa)

6.98

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 90 5e-21 Rx N-terminal domain
NB-ARC PF00931 169 - 342 1.1e-33 NB-ARC domain
WHD_DRP PF23559 422 - 490 2e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 563 - 853 8.9e-31 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 680 - 800 2.5e-33 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 1047 - 1184 3.3e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 3 cut(s) 1779, 2441, 3021
AccB1I GGYRCC 4 cut(s) 1779, 2441, 3021, 3795
AccB7I CCANNNNNTGG 2 cut(s) 265, 574
AccBSI CCGCTC 1 cut(s) 2745
AccII CGCG 1 cut(s) 1644
AciI CCGC 6 cut(s) 185, 1371, 1615, 1644, 2732, 2745
AclI AACGTT 1 cut(s) 1994
AclWI GGATC 5 cut(s) 422, 3545, 3682, 3695, 3695
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 6 cut(s) 329, 1036, 1283, 1583, 1903, 2695
AcuI CTGAAG 4 cut(s) 673, 1787, 3509, 3800
AcyI GRCGYC 2 cut(s) 93, 3796
AdeI CACNNNGTG 1 cut(s) 3638
AfeI AGCGCT 2 cut(s) 2910, 3228
AfiI CCNNNNNNNGG 9 cut(s) 265, 286, 491, 574, 599, 1970, 2357, 3425, 3980
AflII CTTAAG 1 cut(s) 2749
AflIII ACRYGT 1 cut(s) 2208
AjnI CCWGG 4 cut(s) 430, 892, 3690, 3952
AjuI GAANNNNNNNTTGG 2 cut(s) 2511, 2543
Alw21I GWGCWC 3 cut(s) 210, 892, 1716
AlwI GGATC 5 cut(s) 422, 3545, 3682, 3695, 3695
AlwNI CAGNNNCTG 2 cut(s) 40, 2833
Ama87I CYCGRG 4 cut(s) 20, 441, 735, 3418
Aor51HI AGCGCT 2 cut(s) 2910, 3228
AoxI GGCC 8 cut(s) 288, 576, 2047, 3211, 3352, 3810, 3971, 3982
ApeKI GCWGC 6 cut(s) 40, 148, 1009, 1091, 1478, 3742
ApoI RAATTY 6 cut(s) 329, 1036, 1283, 1583, 1903, 2695
ArsI GACNNNNNNTTYG 4 cut(s) 854, 886, 1528, 1560
AseI ATTAAT 1 cut(s) 1911
Asp700I GAANNNNTTC 2 cut(s) 1002, 1525
Asp718I GGTACC 3 cut(s) 1779, 2441, 3021
AspLEI GCGC 3 cut(s) 2911, 3229, 3798
AspS9I GGNCC 5 cut(s) 288, 482, 2048, 3811, 3972
AsuC2I CCSGG 3 cut(s) 594, 736, 737
AsuHPI GGTGA 7 cut(s) 166, 199, 269, 280, 922, 1385, 1472
AsuII TTCGAA 1 cut(s) 1587
AsuNHI GCTAGC 1 cut(s) 3865
AvaI CYCGRG 4 cut(s) 20, 441, 735, 3418
AvaII GGWCC 1 cut(s) 482
BaeI ACNNNNGTAYC 2 cut(s) 2813, 2846
BalI TGGCCA 1 cut(s) 578
BamHI GGATCC 1 cut(s) 3687
BanI GGYRCC 4 cut(s) 1779, 2441, 3021, 3795
BanII GRGCYC 1 cut(s) 210
BarI GAAGNNNNNNTAC 2 cut(s) 1660, 1692
BauI CACGAG 1 cut(s) 918
BbsI GAAGAC 2 cut(s) 510, 977
Bbv12I GWGCWC 3 cut(s) 210, 892, 1716
BbvI GCAGC 6 cut(s) 27, 135, 1021, 1078, 1465, 3729
BccI CCATC 9 cut(s) 473, 1309, 1351, 1511, 1565, 2281, 2496, 3193, 3727
BciT130I CCWGG 4 cut(s) 432, 894, 3692, 3954
BciVI GTATCC 1 cut(s) 3139
BclI TGATCA 1 cut(s) 270
BcnI CCSGG 3 cut(s) 594, 736, 737
BfaI CTAG 6 cut(s) 1935, 1983, 2022, 2165, 2471, 3866
BfmI CTRYAG 3 cut(s) 149, 1479, 2825
BfoI RGCGCY 3 cut(s) 2912, 3230, 3799
BfrI CTTAAG 1 cut(s) 2749
BfuI GTATCC 1 cut(s) 3139
BglII AGATCT 2 cut(s) 775, 2977
BisI GCNGC 7 cut(s) 41, 149, 1010, 1092, 1479, 2746, 3743
BlsI GCNGC 7 cut(s) 42, 150, 1011, 1093, 1480, 2747, 3744
BmcAI AGTACT 5 cut(s) 838, 1203, 1703, 2460, 3164
Bme1390I CCNGG 7 cut(s) 432, 594, 736, 737, 894, 3692, 3954
Bme18I GGWCC 1 cut(s) 482
BmeT110I CYCGRG 4 cut(s) 20, 441, 735, 3418
BmgT120I GGNCC 5 cut(s) 288, 482, 2048, 3811, 3972
BmiI GGNNCC 7 cut(s) 289, 1781, 2050, 2443, 3023, 3689, 3797
BmrFI CCNGG 7 cut(s) 432, 594, 736, 737, 894, 3692, 3954
BmtI GCTAGC 1 cut(s) 3869
BoxI GACNNNNGTC 1 cut(s) 3265
BpiI GAAGAC 2 cut(s) 510, 977
BplI GAGNNNNNCTC 4 cut(s) 109, 141, 3092, 3124
BpmI CTGGAG 3 cut(s) 851, 2483, 3284
Bpu10I CCTNAGC 1 cut(s) 3791
Bpu14I TTCGAA 1 cut(s) 1587
BpuEI CTTGAG 4 cut(s) 716, 2360, 2696, 3179
BpuMI CCSGG 3 cut(s) 594, 736, 737
Bsa29I ATCGAT 1 cut(s) 2103
BsaAI YACGTR 1 cut(s) 3009
BsaBI GATNNNNATC 1 cut(s) 3705
BsaHI GRCGYC 2 cut(s) 93, 3796
BsaJI CCNNGG 5 cut(s) 88, 440, 735, 3691, 3953
BsaXI ACNNNNNCTCC 8 cut(s) 332, 362, 826, 856, 1379, 1409, 3875, 3905
Bsc4I CCNNNNNNNGG 9 cut(s) 265, 286, 491, 574, 599, 1970, 2357, 3425, 3980
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 6 cut(s) 868, 1628, 2456, 2466, 3267, 3974
Bse3DI GCAATG 4 cut(s) 363, 1081, 2156, 2641
Bse8I GATNNNNATC 1 cut(s) 3705
BseBI CCWGG 4 cut(s) 432, 894, 3692, 3954
BseCI ATCGAT 1 cut(s) 2103
BseDI CCNNGG 5 cut(s) 88, 440, 735, 3691, 3953
BseJI GATNNNNATC 1 cut(s) 3705
BseLI CCNNNNNNNGG 9 cut(s) 265, 286, 491, 574, 599, 1970, 2357, 3425, 3980
BseMI GCAATG 4 cut(s) 363, 1081, 2156, 2641
BseMII CTCAG 6 cut(s) 85, 150, 773, 2417, 2825, 3665
BseNI ACTGG 6 cut(s) 868, 1628, 2456, 2466, 3267, 3974
BseRI GAGGAG 4 cut(s) 2196, 3438, 3743, 3896
BseXI GCAGC 6 cut(s) 27, 135, 1021, 1078, 1465, 3729
BseYI CCCAGC 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 1644
Bsh1285I CGRYCG 1 cut(s) 1648
BshFI GGCC 8 cut(s) 290, 578, 2049, 3213, 3354, 3812, 3973, 3984
BshNI GGYRCC 4 cut(s) 1779, 2441, 3021, 3795
BshVI ATCGAT 1 cut(s) 2103
BsiEI CGRYCG 1 cut(s) 1648
BsiHKAI GWGCWC 3 cut(s) 210, 892, 1716
BsiHKCI CYCGRG 4 cut(s) 20, 441, 735, 3418
BsiSI CCGG 4 cut(s) 291, 593, 736, 1748
BslFI GGGAC 2 cut(s) 609, 2083
BslI CCNNNNNNNGG 9 cut(s) 265, 286, 491, 574, 599, 1970, 2357, 3425, 3980
BsmFI GGGAC 2 cut(s) 609, 2083
BsmI GAATGC 3 cut(s) 1962, 2741, 3068
BsnI GGCC 8 cut(s) 290, 578, 2049, 3213, 3354, 3812, 3973, 3984
BsoBI CYCGRG 4 cut(s) 20, 441, 735, 3418
Bsp119I TTCGAA 1 cut(s) 1587
Bsp1286I GDGCHC 3 cut(s) 210, 892, 1716
Bsp1407I TGTACA 1 cut(s) 554
BspACI CCGC 6 cut(s) 185, 1371, 1615, 1644, 2732, 2745
BspANI GGCC 8 cut(s) 290, 578, 2049, 3213, 3354, 3812, 3973, 3984
BspCNI CTCAG 6 cut(s) 84, 151, 774, 2416, 2826, 3666
BspDI ATCGAT 1 cut(s) 2103
BspFNI CGCG 1 cut(s) 1644
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 7 cut(s) 289, 1781, 2050, 2443, 3023, 3689, 3797
BspMAI CTGCAG 3 cut(s) 153, 1483, 2829
BspOI GCTAGC 1 cut(s) 3869
BspPI GGATC 5 cut(s) 422, 3545, 3682, 3695, 3695
BspQI GCTCTTC 1 cut(s) 3040
BspT104I TTCGAA 1 cut(s) 1587
BspT107I GGYRCC 4 cut(s) 1779, 2441, 3021, 3795
BspTI CTTAAG 1 cut(s) 2749
BsrBI CCGCTC 1 cut(s) 2745
BsrDI GCAATG 4 cut(s) 363, 1081, 2156, 2641
BsrFI RCCGGY 1 cut(s) 290
BsrGI TGTACA 1 cut(s) 554
BsrI ACTGG 6 cut(s) 868, 1628, 2456, 2466, 3267, 3974
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 5 cut(s) 88, 440, 735, 3691, 3953
BssNI GRCGYC 2 cut(s) 93, 3796
BssSI CACGAG 1 cut(s) 918
BssT1I CCWWGG 1 cut(s) 88
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 4 cut(s) 432, 894, 3692, 3954
Bst4CI ACNGT 9 cut(s) 1022, 1201, 1473, 1556, 3131, 3290, 3381, 3442, 3548
Bst6I CTCTTC 8 cut(s) 1235, 2390, 2847, 3040, 3121, 3528, 3567, 3935
BstACI GRCGYC 2 cut(s) 93, 3796
BstAFI CTTAAG 1 cut(s) 2749
BstAUI TGTACA 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 3009
BstBI TTCGAA 1 cut(s) 1587
BstC8I GCNNGC 6 cut(s) 292, 1132, 1940, 1988, 3629, 3867
BstFNI CGCG 1 cut(s) 1644
BstH2I RGCGCY 3 cut(s) 2912, 3230, 3799
BstHHI GCGC 3 cut(s) 2911, 3229, 3798
BstMCI CGRYCG 1 cut(s) 1648
BstMWI GCNNNNNNNGC 6 cut(s) 140, 205, 1475, 2123, 2745, 3804
BstNI CCWGG 4 cut(s) 432, 894, 3692, 3954
BstNSI RCATGY 4 cut(s) 626, 1942, 2212, 2958
BstPAI GACNNNNGTC 1 cut(s) 3265
BstSCI CCNGG 7 cut(s) 430, 592, 734, 735, 892, 3690, 3952
BstSFI CTRYAG 3 cut(s) 149, 1479, 2825
BstUI CGCG 1 cut(s) 1644
BstV1I GCAGC 6 cut(s) 27, 135, 1021, 1078, 1465, 3729
BstV2I GAAGAC 2 cut(s) 510, 977
BstX2I RGATCY 4 cut(s) 775, 2977, 3687, 3700
BstXI CCANNNNNNTGG 2 cut(s) 666, 3505
BstYI RGATCY 4 cut(s) 775, 2977, 3687, 3700
Bsu15I ATCGAT 1 cut(s) 2103
BsuI GTATCC 1 cut(s) 3139
BsuRI GGCC 8 cut(s) 290, 578, 2049, 3213, 3354, 3812, 3973, 3984
BsuTUI ATCGAT 1 cut(s) 2103
BtsIMutI CAGTG 3 cut(s) 2709, 3295, 3967
Cac8I GCNNGC 6 cut(s) 292, 1132, 1940, 1988, 3629, 3867
CaiI CAGNNNCTG 2 cut(s) 40, 2833
CciI TCATGA 1 cut(s) 541
CfoI GCGC 3 cut(s) 2911, 3229, 3798
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 5 cut(s) 288, 482, 2048, 3811, 3972
Cfr9I CCCGGG 1 cut(s) 735
ClaI ATCGAT 1 cut(s) 2103
CseI GACGC 1 cut(s) 82
DinI GGCGCC 1 cut(s) 3797
DraIII CACNNNGTG 1 cut(s) 3638
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 8 cut(s) 1235, 2390, 2847, 3040, 3121, 3528, 3567, 3935
EarI CTCTTC 8 cut(s) 1235, 2390, 2847, 3040, 3121, 3528, 3567, 3935
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 88
Eco147I AGGCCT 1 cut(s) 3354
Eco24I GRGCYC 1 cut(s) 210
Eco32I GATATC 2 cut(s) 1489, 1730
Eco47I GGWCC 1 cut(s) 482
Eco47III AGCGCT 2 cut(s) 2910, 3228
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 4 cut(s) 673, 1787, 3509, 3800
Eco88I CYCGRG 4 cut(s) 20, 441, 735, 3418
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 2 cut(s) 2048, 3811
EcoRII CCWGG 4 cut(s) 430, 892, 3690, 3952
EcoRV GATATC 2 cut(s) 1489, 1730
EcoT14I CCWWGG 1 cut(s) 88
EcoT22I ATGCAT 3 cut(s) 1449, 2158, 2741
EcoT38I GRGCYC 1 cut(s) 210
EgeI GGCGCC 1 cut(s) 3797
EheI GGCGCC 1 cut(s) 3797
ErhI CCWWGG 1 cut(s) 88
FalI AAGNNNNNCTT 4 cut(s) 1232, 1264, 2369, 2401
FaqI GGGAC 2 cut(s) 609, 2083
FauNDI CATATG 2 cut(s) 469, 2682
FbaI TGATCA 1 cut(s) 270
Fnu4HI GCNGC 7 cut(s) 41, 149, 1010, 1092, 1479, 2746, 3743
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 7 cut(s) 41, 149, 1010, 1092, 1479, 2746, 3743
FspBI CTAG 6 cut(s) 1935, 1983, 2022, 2165, 2471, 3866
GlaI GCGC 3 cut(s) 2910, 3228, 3797
GluI GCNGC 7 cut(s) 41, 149, 1010, 1092, 1479, 2746, 3743
GsaI CCCAGC 1 cut(s) 262
GsuI CTGGAG 3 cut(s) 851, 2483, 3284
HaeII RGCGCY 3 cut(s) 2912, 3230, 3799
HaeIII GGCC 8 cut(s) 290, 578, 2049, 3213, 3354, 3812, 3973, 3984
HapII CCGG 4 cut(s) 291, 593, 736, 1748
HgaI GACGC 1 cut(s) 82
HhaI GCGC 3 cut(s) 2911, 3229, 3798
Hin1I GRCGYC 2 cut(s) 93, 3796
Hin6I GCGC 3 cut(s) 2909, 3227, 3796
HinP1I GCGC 3 cut(s) 2909, 3227, 3796
HincII GTYRAC 3 cut(s) 54, 1976, 2662
HindII GTYRAC 3 cut(s) 54, 1976, 2662
HindIII AAGCTT 1 cut(s) 3489
HpaII CCGG 4 cut(s) 291, 593, 736, 1748
HphI GGTGA 7 cut(s) 166, 199, 269, 280, 922, 1385, 1472
Hpy166II GTNNAC 6 cut(s) 54, 1976, 2573, 2662, 3257, 3613
Hpy8I GTNNAC 6 cut(s) 54, 1976, 2573, 2662, 3257, 3613
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 9 cut(s) 1022, 1201, 1473, 1556, 3131, 3290, 3381, 3442, 3548
HpyCH4IV ACGT 6 cut(s) 1117, 1850, 1918, 1994, 3008, 3622
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 205, 1475, 2123, 2745, 3804
HpySE526I ACGT 6 cut(s) 1117, 1850, 1918, 1994, 3008, 3622
Hsp92I GRCGYC 2 cut(s) 93, 3796
HspAI GCGC 3 cut(s) 2909, 3227, 3796
KasI GGCGCC 1 cut(s) 3795
KpnI GGTACC 3 cut(s) 1783, 2445, 3025
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 270
LguI GCTCTTC 1 cut(s) 3040
LmnI GCTCC 4 cut(s) 887, 947, 1387, 3750
Lsp1109I GCAGC 6 cut(s) 27, 135, 1021, 1078, 1465, 3729
MaeI CTAG 6 cut(s) 1935, 1983, 2022, 2165, 2471, 3866
MaeII ACGT 6 cut(s) 1117, 1850, 1918, 1994, 3008, 3622
MbiI CCGCTC 1 cut(s) 2745
MfeI CAATTG 5 cut(s) 1755, 2576, 2882, 3472, 3922
MflI RGATCY 4 cut(s) 775, 2977, 3687, 3700
MhlI GDGCHC 3 cut(s) 210, 892, 1716
MlsI TGGCCA 1 cut(s) 578
MluNI TGGCCA 1 cut(s) 578
Mly113I GGCGCC 1 cut(s) 3796
MlyI GAGTC 5 cut(s) 1093, 1531, 2036, 3275, 3657
MmeI TCCRAC 4 cut(s) 456, 472, 865, 3471
Mox20I TGGCCA 1 cut(s) 578
Mph1103I ATGCAT 3 cut(s) 1449, 2158, 2741
MroNI GCCGGC 1 cut(s) 290
MroXI GAANNNNTTC 2 cut(s) 1002, 1525
MscI TGGCCA 1 cut(s) 578
MslI CAYNNNNRTG 5 cut(s) 282, 960, 2105, 2281, 2800
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 2893
MspCI CTTAAG 1 cut(s) 2749
MspI CCGG 4 cut(s) 291, 593, 736, 1748
MspR9I CCNGG 7 cut(s) 432, 594, 736, 737, 894, 3692, 3954
MunI CAATTG 5 cut(s) 1755, 2576, 2882, 3472, 3922
Mva1269I GAATGC 3 cut(s) 1962, 2741, 3068
MvaI CCWGG 4 cut(s) 432, 894, 3692, 3954
MvnI CGCG 1 cut(s) 1644
MwoI GCNNNNNNNGC 6 cut(s) 140, 205, 1475, 2123, 2745, 3804
NaeI GCCGGC 1 cut(s) 292
NarI GGCGCC 1 cut(s) 3796
NciI CCSGG 3 cut(s) 594, 736, 737
NdeI CATATG 2 cut(s) 469, 2682
NgoMIV GCCGGC 1 cut(s) 290
NheI GCTAGC 1 cut(s) 3865
NlaIV GGNNCC 7 cut(s) 289, 1781, 2050, 2443, 3023, 3689, 3797
NmeAIII GCCGAG 1 cut(s) 486
NmuCI GTSAC 5 cut(s) 80, 727, 842, 910, 1373
NsiI ATGCAT 3 cut(s) 1449, 2158, 2741
NspI RCATGY 4 cut(s) 626, 1942, 2212, 2958
NspV TTCGAA 1 cut(s) 1587
PacI TTAATTAA 1 cut(s) 1911
PaeI GCATGC 1 cut(s) 1942
PagI TCATGA 1 cut(s) 541
PceI AGGCCT 1 cut(s) 3354
PciI ACATGT 1 cut(s) 2208
PciSI GCTCTTC 1 cut(s) 3040
PctI GAATGC 3 cut(s) 1962, 2741, 3068
PdiI GCCGGC 1 cut(s) 292
PdmI GAANNNNTTC 2 cut(s) 1002, 1525
PflMI CCANNNNNTGG 2 cut(s) 265, 574
PkrI GCNGC 7 cut(s) 42, 150, 1011, 1093, 1480, 2747, 3744
PleI GAGTC 5 cut(s) 1093, 1531, 2035, 3274, 3657
PluTI GGCGCC 1 cut(s) 3799
PpsI GAGTC 5 cut(s) 1093, 1531, 2035, 3274, 3657
Ppu21I YACGTR 1 cut(s) 3009
PscI ACATGT 1 cut(s) 2208
PshAI GACNNNNGTC 1 cut(s) 3265
PshBI ATTAAT 1 cut(s) 1911
Psp124BI GAGCTC 1 cut(s) 210
Psp1406I AACGTT 1 cut(s) 1994
Psp6I CCWGG 4 cut(s) 430, 892, 3690, 3952
PspFI CCCAGC 1 cut(s) 258
PspGI CCWGG 4 cut(s) 430, 892, 3690, 3952
PspN4I GGNNCC 7 cut(s) 289, 1781, 2050, 2443, 3023, 3689, 3797
PspPI GGNCC 5 cut(s) 288, 482, 2048, 3811, 3972
PstI CTGCAG 3 cut(s) 153, 1483, 2829
PstNI CAGNNNCTG 2 cut(s) 40, 2833
PsuI RGATCY 4 cut(s) 775, 2977, 3687, 3700
PvuII CAGCTG 1 cut(s) 2893
RseI CAYNNNNRTG 5 cut(s) 282, 960, 2105, 2281, 2800
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 3040
SatI GCNGC 7 cut(s) 41, 149, 1010, 1092, 1479, 2746, 3743
Sau96I GGNCC 5 cut(s) 288, 482, 2048, 3811, 3972
ScaI AGTACT 5 cut(s) 838, 1203, 1703, 2460, 3164
SchI GAGTC 5 cut(s) 1093, 1531, 2036, 3275, 3657
ScrFI CCNGG 7 cut(s) 432, 594, 736, 737, 894, 3692, 3954
SduI GDGCHC 3 cut(s) 210, 892, 1716
SfcI CTRYAG 3 cut(s) 149, 1479, 2825
SfoI GGCGCC 1 cut(s) 3797
SfuI TTCGAA 1 cut(s) 1587
SinI GGWCC 1 cut(s) 482
SmaI CCCGGG 1 cut(s) 737
SmiMI CAYNNNNRTG 5 cut(s) 282, 960, 2105, 2281, 2800
SmlI CTYRAG 5 cut(s) 695, 2375, 2675, 2749, 3158
SmoI CTYRAG 5 cut(s) 695, 2375, 2675, 2749, 3158
SphI GCATGC 1 cut(s) 1942
SseBI AGGCCT 1 cut(s) 3354
SsiI CCGC 6 cut(s) 185, 1371, 1615, 1644, 2732, 2745
SspDI GGCGCC 1 cut(s) 3795
SspMI CTAG 6 cut(s) 1935, 1983, 2022, 2165, 2471, 3866
SstI GAGCTC 1 cut(s) 210
StuI AGGCCT 1 cut(s) 3354
StyD4I CCNGG 7 cut(s) 430, 592, 734, 735, 892, 3690, 3952
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 9 cut(s) 1022, 1201, 1473, 1556, 3131, 3290, 3381, 3442, 3548
TaiI ACGT 6 cut(s) 1120, 1853, 1921, 1997, 3011, 3625
TaqI TCGA 8 cut(s) 96, 225, 320, 1547, 1587, 2103, 2341, 3303
TaqII GACCGA 2 cut(s) 68, 1389
TatI WGTACW 8 cut(s) 119, 554, 836, 1201, 1701, 2458, 3162, 3513
TauI GCSGC 1 cut(s) 2748
TscAI CASTG 3 cut(s) 2709, 3295, 3974
TseFI GTSAC 5 cut(s) 80, 727, 842, 910, 1373
TseI GCWGC 6 cut(s) 40, 148, 1009, 1091, 1478, 3742
Tsp45I GTSAC 5 cut(s) 80, 727, 842, 910, 1373
TspDTI ATGAA 9 cut(s) 359, 456, 516, 1296, 1322, 1455, 1712, 2377, 2790
TspMI CCCGGG 1 cut(s) 735
TspRI CASTG 3 cut(s) 2709, 3295, 3974
Van91I CCANNNNNTGG 2 cut(s) 265, 574
Vha464I CTTAAG 1 cut(s) 2749
VpaK11BI GGWCC 1 cut(s) 482
VspI ATTAAT 1 cut(s) 1911
XapI RAATTY 6 cut(s) 329, 1036, 1283, 1583, 1903, 2695
XceI RCATGY 4 cut(s) 626, 1942, 2212, 2958
XcmI CCANNNNNNNNNTGG 3 cut(s) 262, 2633, 3639
XmaI CCCGGG 1 cut(s) 735
XmnI GAANNNNTTC 2 cut(s) 1002, 1525
XspI CTAG 6 cut(s) 1935, 1983, 2022, 2165, 2471, 3866
ZrmI AGTACT 5 cut(s) 838, 1203, 1703, 2460, 3164
Zsp2I ATGCAT 3 cut(s) 1449, 2158, 2741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.