Rh4BG230900

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011033
Physical Location & Seq
Forward (+)
39526335 .. 39532116
5782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG230900.1

Sequence Viewer

Length: 4095 bp
ATGAACCAGTACCACATATACGAGGCCATCGGCCGTGGAAAGTGCTCGACTGTGTACAAAGGGAGGAAGAAGAAGACGATTGAGTACTTCGCCATTAAGAGCGTCGAGAAATCTCAGAAGAGCAAGCTTCTTCAGGAAGTGAAGATTCTTCATACCCTAGATCATCAGAATATTCTCAAGTTTTATTGGTGGTACGAAACTTCTGCTCACTTGTGGTTAGTTCTGGAGTATTGCGTTGGCGGGAATTTGATGACATTGTTAGACCAGGATTGTCAGCTACCTGAAGAGTCGATTCATGACCTTGGGTGGGACCTTGTTAGAGCTTTGCTGTTTTTACATTCGAAGGGAATCATATACTGTGATTTGAAACCATCGAATATCCTGTTGGATGAAAATGGCCGTACAAAGCTCTGTGATTTTGGATTGGCTAGAAAGCTGAGCGAAATATCACAAACCTCTTCTTCCTCGTTGCCACAAGCAAAACGTGGAACACCTTGTTATATGGCTCCTGAGCTTTTTGAAGACGGGGGTGTCCATTCATATGCATCTGATTTCTGGGCCCTTGGTTGTGTACTATATGAGTGCTATGCTGGGAAGCCTCCCTTTGTGGGGAGAGAATTTACTCAGCTAGTGAAGTCCATCCTCTCAGATCCAATACCTCCTCTACCTGGCACTCCTATTCGTCCTTTTGTCAATCTAATAAATTCTTTACTCATCAAGGATCCAGCTGAACGAATACAGTGGACTGAACTTTGTGGTCATGCTTTCTGGAGAACCAAGTTAAATCTAGTGCCTCTACCTCCTCAGCCTGCTTTTGCAAATATGCTAGAGCACTATGCTAAGCCATTGTCAATACGTAATGGTGAGAACAGGACTCCTAAATCTCGCCAAAACGAGGTTAAAGGAGCTTCAAAGCAGGATGAGAATTCTGTAGTAGGATCAAAAGGCCATGGAACTCCAGTCAAGGGCACACCTGGAAGTCGGAGAACTCAGGCAAAGGTATCTGGCAGAGCAGTTGATGAAAAGCAAAAGGATCCTTCTGGTACTACCAGGGGTGTGAATCTTTTGAGACTCTCAAGAATAGCAAAGAAGAACTTGCAGAGGGAAAATGAGAAAGAGAACTACCGGAGGCTAGTGTCTACTAATTCTGAGAATGATTCAGAAGTCAAAATTGAGAACACTGATATGGAACTTGATTTTAATGAAAACACTGAAGATGATGCACAGGATGAATCTGATGGGCCAGATAACCAAGCTTCACTGGATGACAAATCTTCAAGTCAAAATCAGGAGCAGGTTAAAGTGGAAGAGATGGAAAATAACATAAACCAATCAGATACCTCGCCTGTGGTAAATGCTTCTGCATCAAATGAATCAACAGCATGTGATCAAGAATCACCACAGAATATTGAAGTGGCTGCAATTCCATGCAGTGCCAGTCCTCATATAAAAAATCAGAGATTTAAGGAAGGTTCTGGATCTGCAGTTGACTATGATTCTGCAAAATCGTCAAATAACCTTTCAGAGGTTCTTTGGCATCCATCTGATCTTTCAGTCAGACCTGTAATGCCTAACAGAAAAGCTGATAAAACTTCGGAGGTAATTCCTTCACTTCCCTTTGAGGCACTGCAAGCTTCTGATTTTGTGAAGATGTCTAAAGAGCAATTGGATGCACTTACCAATAAGATTATAGCCATTTTTAATGGGAACATTAGCATTGGGGAGAAGCAGAATGCACTTAGATACCTTGAGATGTTAACCAACAATGCGGATGCCGCTAATATCTTGACCAATGGACCCATCATGCTTATCCTTGTTAAAATGCTTCGACAGACCAAGGCTTTGGCCTTGCGTGTCCAAGTTGCTTCACTGATTGGTTTGTTGATTCGACATTCTACTTTTATTCATGATGATTTGGCAAATTCTGGAATTTTGGGTTCACTGAGCGATGGCCTTGGAGACAGGCAGGAAAAAGTGAGGAGATTTTCTATGGCTGCTTTAGGTGAGCTGCTATTCTATATTTCAACTCAAAGTGAGCAAGCTGGAGATAACAATCCTACTGAGTCTCCATCCAAGGAAAGCCGGTCCACGTCTGGTTGGCAGGTCTCCAATTTATTGCTTTCCAAAGTGGCATCAATTTTACAAAAAGGAGAGGATGATTTAACTCAGCTTTATGCATTGAGGACGATTGAAAATATCTGTAGCCAAGCAGGGCACTGGGCAGCTCGTTTGAACAGCCAGGGCATGATTAATAACCTGTGCTACATATATAGGGCTGCTGGGAAACAGGAGAGCATGAGACTTACAGCAGGATCATGTTTGGTCCGTCTTGTTCGTTTCAATCCACCTAGCATTCAGCAAGTAATTGATATTCTTTCTTTAAAGGAAATAGCATCTGCAATTATCAAGGGCAGTCTGAGGGAGCAGCAAATCAGCTTAAACCTCTTGAACATGGCCATGCTTGGAAGTCACATGTTCACAAACTTTGGCAGGCACCTCCTGCCACTGATGGAGGATAAGAATATTGTTCCAAGTCTTGTGTCTCTTATTGAGCAGGGAAGTGAAGTTTTGAAAGGGAAAGCGCTTGTTTTTGTTGCTCTTCTTTGTAAAAACAGCAGTTCCAAGAGCAGTAGGAGGTGGCTGCCACATTTTTTTTGCAATGCCAGGTTACTGTCTATGGTGGACAGGCTGGCAAAAGAGAAAGATCAGTATGTGAGGCAGTGTTTAGATGCATTTGTGTGTGTTGTGGCATCTGTTATACCTGGTTTACTGGATACTATAACTGGTGACATTCAGCAATCAATGGGAAGGCGCCATGTACATCTATCTCCCCTGAACAGTAGAGCTACTCCTAAGACCAACATTCATATGCTCCCTGTAGTTCTCCATCTTCTTCGAAGCTCGTCTTTTAAGCATAAGGTGGTGAGTGATCAGGTCCTGCAGCAGTTGGCAAATTTAATAAAAATCGTGGAAACACCATTTCAGGGCAGGGATGACTTTCAAATCACTCTTCTTCGAATTCTTGAATCTGTTGCCGAGGAGTCCCTTGTAGTTCTCAAAAGCCCTGATATTTTTACCCATGAGGTTCTCCCCAGTTTGGCTGTTCTGTATAAGGGCAACAAGGATGGTGACGCCAGGTTTCTGTGTTTGAAAATCTTGTTTGATGTGATGGTAATTTTTCTTAATGAACAATCTGAAGATGAGCAAAGGTCCAAAGAATTGCAATCCATATCCAATACAAACTTTCTTCCTCTCTACCCTTCCTTGATTGAAGACGAAGATCCAATCCCCTTGTACGCGCAAAAGCTGCTTGTGATGCTGATTGATTATAATTACATCAAAATTGCAGACATTCTACATCTGAAGATAGTCTCGCAATGCTTTGAGTTTTTGCTGGGCGATCTCTCAAGTGCAAATGTGAACAATGTTATGCTATGCCTGGCTCTGACATCTGCTCCTGAAATGGAAACCAAGTTACTTTCTCAGCTGAGGGTAGTTAGGAAAATAGGAAATCTTCTAGAGCTTGTATATGCAAAAGATATGGAAGATTTTTTGGAACCAACTCTTGGTCTGTGTAGGGCTTTCCTTTTACGTTCAGTGAGTGGTAGAAAAGGCTTCACCTATTCAACAGAACCTGCTCTTTTGGGTGATGTGTCTTCTGAGCCAAGTGGTGATCAGCAGCAAGGTATAAGAGATATCATGGACTTTGCCAGCAATGTTGGTGTATTGCTAGAGTTAAGTAGGTCTCATGTAGGAAATGTGGCAGATATAGCTTCAGAATGTGTTGTTTTGTTGCTCAAGGCAGCTCCAAGGGAAGGTACTGCTGGTCTACTAACTAATCTTCCAAAAGTTACTGCAATGCTTGAGTCTTGGCGTAGAGGCACATCTCATTTGTTGGTGCAGCGAGTGCTACATGCACTTGGTTATGCTTGTAGGCAGTATTTGTTACATGCAATGATAATGTCGATATCGATACCTGAAATTCCAAGAATTGAGGGCATAGTTTCTGAGCTTAAAAGTTCAGGTGTACCTGCTTTAGCCACTGCTGCTTTTCATGTGGCTGTGGAGTTGCAGCGGCTTCCTCGCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000912 GO:0000914 GO:0001932 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0007349 GO:0007399 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009653 GO:0009966 GO:0009987 GO:0010245 GO:0010342 GO:0010646 GO:0010975 GO:0015630 GO:0015631 GO:0016043 GO:0019220 GO:0019222 GO:0019953 GO:0022008 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0030054 GO:0030154 GO:0030334 GO:0031323 GO:0031344 GO:0031399 GO:0032268 GO:0032501 GO:0032502 GO:0032506 GO:0032872 GO:0032879 GO:0034293 GO:0040012 GO:0042325 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043408 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045595 GO:0045664 GO:0046328 GO:0048229 GO:0048236 GO:0048583 GO:0048646 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051246 GO:0051270 GO:0051301 GO:0051321 GO:0051704 GO:0051960 GO:0055044 GO:0060255 GO:0060284 GO:0061640 GO:0065007 GO:0070302 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090036 GO:0120035 GO:1900744 GO:1902407 GO:1902410 GO:1902531 GO:1903046 GO:1903047 GO:2000026 GO:2000145 GO:2001222
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1364

Amino Acids

151.69

Weight (kDa)

6.61

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 256 5.9e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 251 3e-37 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 66 - 151 8.5e-07 ABC1 atypical kinase-like domain
HEAT_ULK4 PF23606 923 - 1115 4.4e-71 Serine/threonine-protein kinase ULK4-like, HEAT repeats
ARM_RUK PF24970 1122 - 1363 6.9e-118 RUNKEL ARM-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33940 AT1G33940 AT5G18700
fragaria_vesca FvH4_4g17050 FvH4_4g17050
malus_domestica MD13G1174600.v1.1 MD16G1176300.v1.1
prunus_persica Prupe.1G143100_v2.0.a1
pyrus_communis pycom13g15030 pycom16g14770
rosa_chinensis RchiOBHm_Chr4g0420851
rosa_laevigata RLG00000007733
rosa_multiflora Rmu_sc0002006.1_g000017
rosa_roxburghii Rroxscaffold_5G00363260
rosa_rugosa Rorug04G0166400
rosa_samantha Rh4AG227800 Rh4BG230900 Rh4CG242800 Rh4DG226100
rosa_wichuraiana Rw4G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3330
AasI GACNNNNNNGTC 1 cut(s) 530
Acc36I ACCTGC 4 cut(s) 1285, 2092, 3643, 4039
AccB1I GGYRCC 2 cut(s) 2493, 2811
AccB7I CCANNNNNTGG 1 cut(s) 3566
AccI GTMKAC 2 cut(s) 1139, 3829
AccII CGCG 1 cut(s) 3299
AciI CCGC 4 cut(s) 240, 1769, 1776, 4075
AclWI GGATC 9 cut(s) 644, 716, 729, 946, 1028, 1041, 1486, 2320, 3275
AcoI YGGCCR 3 cut(s) 31, 397, 2454
AcsI RAATTY 9 cut(s) 244, 617, 703, 925, 1921, 1929, 2953, 3018, 3981
AcuI CTGAAG 6 cut(s) 116, 303, 1233, 3216, 3383, 3759
AcyI GRCGYC 2 cut(s) 2812, 3132
AfeI AGCGCT 1 cut(s) 2583
AflIII ACRYGT 1 cut(s) 2472
AjiI CACGTC 1 cut(s) 2091
AjnI CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2663, 2761, 3134, 3438
AjuI GAANNNNNNNTTGG 4 cut(s) 368, 400, 3549, 3581
Alw21I GWGCWC 2 cut(s) 47, 834
Alw26I GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3376, 3750
AlwI GGATC 9 cut(s) 644, 716, 729, 946, 1028, 1041, 1486, 2320, 3275
AlwNI CAGNNNCTG 2 cut(s) 2938, 3635
Aor51HI AGCGCT 1 cut(s) 2583
AoxI GGCC 9 cut(s) 24, 31, 397, 558, 946, 1241, 1845, 1951, 2454
ApaI GGGCCC 1 cut(s) 562
ApoI RAATTY 9 cut(s) 244, 617, 703, 925, 1921, 1929, 2953, 3018, 3981
ArsI GACNNNNNNTTYG 2 cut(s) 1825, 1857
AseI ATTAAT 1 cut(s) 2250
AspLEI GCGC 3 cut(s) 2584, 2814, 3301
AspS9I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2935, 3210
AsuHPI GGTGA 9 cut(s) 875, 1389, 2015, 2798, 2935, 3140, 3610, 3659, 3683
AsuII TTCGAA 3 cut(s) 341, 2896, 3016
AvaII GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2935, 3210
BaeGI GKGCMC 3 cut(s) 562, 971, 2217
BalI TGGCCA 1 cut(s) 2456
BamHI GGATCC 2 cut(s) 721, 1033
BanI GGYRCC 2 cut(s) 2493, 2811
BanII GRGCYC 1 cut(s) 562
BbsI GAAGAC 4 cut(s) 80, 528, 3279, 3648
Bbv12I GWGCWC 2 cut(s) 47, 834
BbvCI CCTCAGC 2 cut(s) 804, 3488
BceAI ACGGC 2 cut(s) 18, 384
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
BciVI GTATCC 1 cut(s) 2767
BclI TGATCA 3 cut(s) 1387, 2929, 3673
BcoDI GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3376, 3750
BfaI CTAG 9 cut(s) 158, 429, 629, 788, 827, 1133, 2349, 3518, 3731
BfmI CTRYAG 5 cut(s) 930, 1482, 2200, 2877, 2939
BfoI RGCGCY 2 cut(s) 2585, 2815
BfuAI ACCTGC 4 cut(s) 1285, 2092, 3643, 4039
BfuI GTATCC 1 cut(s) 2767
BlpI GCTNAGC 2 cut(s) 437, 840
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
Bme18I GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2935, 3210
BmgBI CACGTC 1 cut(s) 2091
BmgT120I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2935, 3210
BmiI GGNNCC 9 cut(s) 311, 507, 560, 723, 1035, 1798, 2495, 2813, 3558
BmrFI CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
BmrI ACTGGG 2 cut(s) 2227, 3087
BmuI ACTGGG 2 cut(s) 2227, 3087
BpiI GAAGAC 4 cut(s) 80, 528, 3279, 3648
BpmI CTGGAG 4 cut(s) 245, 790, 942, 2064
Bpu10I CCTNAGC 3 cut(s) 510, 804, 3488
Bpu1102I GCTNAGC 2 cut(s) 437, 840
Bpu14I TTCGAA 3 cut(s) 341, 2896, 3016
BpuEI CTTGAG 6 cut(s) 161, 1060, 1769, 3391, 3782, 3884
Bsa29I ATCGAT 1 cut(s) 3971
BsaAI YACGTR 1 cut(s) 857
BsaBI GATNNNNATC 1 cut(s) 2052
BsaHI GRCGYC 2 cut(s) 2812, 3132
BsaI GGTCTC 2 cut(s) 2110, 3750
BsaWI WCCGGW 1 cut(s) 1125
BsaXI ACNNNNNCTCC 4 cut(s) 2050, 2080, 3439, 3469
Bse118I RCCGGY 1 cut(s) 2082
Bse1I ACTGG 8 cut(s) 7, 959, 1266, 1437, 2222, 2775, 2788, 3093
Bse3DI GCAATG 5 cut(s) 2665, 3383, 3721, 3864, 3960
Bse8I GATNNNNATC 1 cut(s) 2052
BseBI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
BseCI ATCGAT 1 cut(s) 3971
BseJI GATNNNNATC 1 cut(s) 2052
BseMI GCAATG 5 cut(s) 2665, 3383, 3721, 3864, 3960
BseNI ACTGG 8 cut(s) 7, 959, 1266, 1437, 2222, 2775, 2788, 3093
BseRI GAGGAG 4 cut(s) 651, 792, 1993, 3053
BseSI GKGCMC 3 cut(s) 562, 971, 2217
BseX3I CGGCCG 1 cut(s) 31
BseYI CCCAGC 3 cut(s) 590, 2279, 3394
BsgI GTGCAG 1 cut(s) 3920
Bsh1236I CGCG 1 cut(s) 3299
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 9 cut(s) 26, 33, 399, 560, 948, 1243, 1847, 1953, 2456
BshNI GGYRCC 2 cut(s) 2493, 2811
BshVI ATCGAT 1 cut(s) 3971
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 2 cut(s) 47, 834
BsiSI CCGG 2 cut(s) 1126, 2083
BslFI GGGAC 2 cut(s) 323, 3028
BsmAI GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3376, 3750
BsmFI GGGAC 2 cut(s) 323, 3028
BsmI GAATGC 2 cut(s) 1738, 2352
BsnI GGCC 9 cut(s) 26, 33, 399, 560, 948, 1243, 1847, 1953, 2456
Bso31I GGTCTC 2 cut(s) 2110, 3750
Bsp119I TTCGAA 3 cut(s) 341, 2896, 3016
Bsp120I GGGCCC 1 cut(s) 558
Bsp1286I GDGCHC 5 cut(s) 47, 562, 834, 971, 2217
Bsp1407I TGTACA 2 cut(s) 54, 2818
Bsp1720I GCTNAGC 2 cut(s) 437, 840
Bsp19I CCATGG 1 cut(s) 949
BspACI CCGC 4 cut(s) 240, 1769, 1776, 4075
BspANI GGCC 9 cut(s) 26, 33, 399, 560, 948, 1243, 1847, 1953, 2456
BspDI ATCGAT 1 cut(s) 3971
BspFNI CGCG 1 cut(s) 3299
BspHI TCATGA 2 cut(s) 295, 1906
BspLI GGNNCC 9 cut(s) 311, 507, 560, 723, 1035, 1798, 2495, 2813, 3558
BspMAI CTGCAG 2 cut(s) 1486, 2943
BspMI ACCTGC 4 cut(s) 1285, 2092, 3643, 4039
BspPI GGATC 9 cut(s) 644, 716, 729, 946, 1028, 1041, 1486, 2320, 3275
BspQI GCTCTTC 2 cut(s) 113, 2604
BspT104I TTCGAA 3 cut(s) 341, 2896, 3016
BspT107I GGYRCC 2 cut(s) 2493, 2811
BspTNI GGTCTC 2 cut(s) 2110, 3750
BsrDI GCAATG 5 cut(s) 2665, 3383, 3721, 3864, 3960
BsrFI RCCGGY 1 cut(s) 2082
BsrGI TGTACA 2 cut(s) 54, 2818
BsrI ACTGG 8 cut(s) 7, 959, 1266, 1437, 2222, 2775, 2788, 3093
BssAI RCCGGY 1 cut(s) 2082
BssNI GRCGYC 2 cut(s) 2812, 3132
BssT1I CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2073, 3809
Bst2UI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
Bst4CI ACNGT 5 cut(s) 52, 359, 741, 2673, 2840
Bst6I CTCTTC 6 cut(s) 113, 279, 463, 1302, 2604, 3015
BstACI GRCGYC 2 cut(s) 2812, 3132
BstAPI GCANNNNNTGC 3 cut(s) 2500, 3307, 3907
BstAUI TGTACA 2 cut(s) 54, 2818
BstBAI YACGTR 1 cut(s) 857
BstBI TTCGAA 3 cut(s) 341, 2896, 3016
BstC8I GCNNGC 7 cut(s) 125, 810, 1632, 2040, 2492, 2691, 3712
BstDSI CCRYGG 2 cut(s) 34, 949
BstENI CCTNNNNNAGG 1 cut(s) 1523
BstFNI CGCG 1 cut(s) 3299
BstH2I RGCGCY 2 cut(s) 2585, 2815
BstHHI GCGC 3 cut(s) 2584, 2814, 3301
BstMAI GTCTC 8 cut(s) 1063, 1953, 2070, 2110, 2293, 2547, 3376, 3750
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 8 cut(s) 1631, 1775, 2500, 3307, 3316, 3770, 3907, 4046
BstNI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
BstNSI RCATGY 5 cut(s) 1386, 2476, 3917, 3953, 4093
BstSCI CCNGG 9 cut(s) 264, 667, 973, 1049, 2238, 2663, 2761, 3134, 3438
BstSFI CTRYAG 5 cut(s) 930, 1482, 2200, 2877, 2939
BstSLI GKGCMC 3 cut(s) 562, 971, 2217
BstSNI TACGTA 1 cut(s) 857
BstUI CGCG 1 cut(s) 3299
BstV2I GAAGAC 4 cut(s) 80, 528, 3279, 3648
BstX2I RGATCY 5 cut(s) 649, 721, 1033, 1478, 3280
BstXI CCANNNNNNTGG 1 cut(s) 1843
BstYI RGATCY 5 cut(s) 649, 721, 1033, 1478, 3280
BstZI CGGCCG 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 3971
BsuI GTATCC 1 cut(s) 2767
BsuRI GGCC 9 cut(s) 26, 33, 399, 560, 948, 1243, 1847, 1953, 2456
BsuTUI ATCGAT 1 cut(s) 3971
BtgI CCRYGG 2 cut(s) 34, 949
BtgZI GCGATG 1 cut(s) 1962
BtrI CACGTC 1 cut(s) 2091
BtsI GCAGTG 4 cut(s) 1438, 1625, 2726, 4041
BveI ACCTGC 4 cut(s) 1285, 2092, 3643, 4039
Cac8I GCNNGC 7 cut(s) 125, 810, 1632, 2040, 2492, 2691, 3712
CaiI CAGNNNCTG 2 cut(s) 2938, 3635
CciI TCATGA 2 cut(s) 295, 1906
CfoI GCGC 3 cut(s) 2584, 2814, 3301
Cfr10I RCCGGY 1 cut(s) 2082
Cfr13I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2935, 3210
ClaI ATCGAT 1 cut(s) 3971
CseI GACGC 2 cut(s) 91, 3140
CsiI ACCWGGT 1 cut(s) 2761
DinI GGCGCC 1 cut(s) 2813
DraI TTTAAA 1 cut(s) 2382
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 3 cut(s) 31, 397, 2454
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 6 cut(s) 113, 279, 463, 1302, 2604, 3015
EarI CTCTTC 6 cut(s) 113, 279, 463, 1302, 2604, 3015
EclXI CGGCCG 1 cut(s) 31
Eco105I TACGTA 1 cut(s) 857
Eco130I CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2073, 3809
Eco24I GRGCYC 1 cut(s) 562
Eco31I GGTCTC 2 cut(s) 2110, 3750
Eco32I GATATC 2 cut(s) 3697, 3969
Eco47I GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2935, 3210
Eco47III AGCGCT 1 cut(s) 2583
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 6 cut(s) 116, 303, 1233, 3216, 3383, 3759
EcoNI CCTNNNNNAGG 1 cut(s) 1523
EcoO109I RGGNCCY 3 cut(s) 310, 559, 2935
EcoRI GAATTC 2 cut(s) 925, 3018
EcoRII CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2663, 2761, 3134, 3438
EcoRV GATATC 2 cut(s) 3697, 3969
EcoT14I CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2073, 3809
EcoT22I ATGCAT 3 cut(s) 547, 2179, 2734
EcoT38I GRGCYC 1 cut(s) 562
EgeI GGCGCC 1 cut(s) 2813
EheI GGCGCC 1 cut(s) 2813
ErhI CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2073, 3809
FalI AAGNNNNNCTT 6 cut(s) 587, 619, 1079, 1111, 2890, 2922
FaqI GGGAC 2 cut(s) 323, 3028
FauI CCCGC 1 cut(s) 233
FauNDI CATATG 2 cut(s) 541, 2868
FbaI TGATCA 3 cut(s) 1387, 2929, 3673
FblI GTMKAC 2 cut(s) 1139, 3829
FriOI GRGCYC 1 cut(s) 562
FspBI CTAG 9 cut(s) 158, 429, 629, 788, 827, 1133, 2349, 3518, 3731
GlaI GCGC 3 cut(s) 2583, 2813, 3300
GsaI CCCAGC 3 cut(s) 594, 2283, 3398
GsuI CTGGAG 4 cut(s) 245, 790, 942, 2064
HaeII RGCGCY 2 cut(s) 2585, 2815
HaeIII GGCC 9 cut(s) 26, 33, 399, 560, 948, 1243, 1847, 1953, 2456
HapII CCGG 2 cut(s) 1126, 2083
HgaI GACGC 2 cut(s) 91, 3140
HhaI GCGC 3 cut(s) 2584, 2814, 3301
Hin1I GRCGYC 2 cut(s) 2812, 3132
Hin6I GCGC 3 cut(s) 2582, 2812, 3299
HinP1I GCGC 3 cut(s) 2582, 2812, 3299
HincII GTYRAC 2 cut(s) 1489, 1758
HindII GTYRAC 2 cut(s) 1489, 1758
HindIII AAGCTT 3 cut(s) 125, 1254, 1632
HpaI GTTAAC 1 cut(s) 1758
HpaII CCGG 2 cut(s) 1126, 2083
HphI GGTGA 9 cut(s) 875, 1389, 2015, 2798, 2935, 3140, 3610, 3659, 3683
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 7 cut(s) 337, 1047, 1463, 1617, 2802, 3270, 3809
HpyCH4III ACNGT 5 cut(s) 52, 359, 741, 2673, 2840
HpyCH4IV ACGT 4 cut(s) 484, 856, 2090, 3592
HpyF10VI GCNNNNNNNGC 8 cut(s) 1631, 1775, 2500, 3307, 3316, 3770, 3907, 4046
HpySE526I ACGT 4 cut(s) 484, 856, 2090, 3592
Hsp92I GRCGYC 2 cut(s) 2812, 3132
HspAI GCGC 3 cut(s) 2582, 2812, 3299
KasI GGCGCC 1 cut(s) 2811
Ksp22I TGATCA 3 cut(s) 1387, 2929, 3673
KspAI GTTAAC 1 cut(s) 1758
LguI GCTCTTC 2 cut(s) 113, 2604
LmnI GCTCC 7 cut(s) 511, 905, 1291, 2422, 2877, 3460, 3811
MabI ACCWGGT 1 cut(s) 2761
MaeI CTAG 9 cut(s) 158, 429, 629, 788, 827, 1133, 2349, 3518, 3731
MaeII ACGT 4 cut(s) 484, 856, 2090, 3592
MaeIII GTNAC 7 cut(s) 2468, 2667, 2786, 3128, 3474, 3850, 3945
MfeI CAATTG 1 cut(s) 1664
MflI RGATCY 5 cut(s) 649, 721, 1033, 1478, 3280
MhlI GDGCHC 5 cut(s) 47, 562, 834, 971, 2217
MlsI TGGCCA 1 cut(s) 2456
MluNI TGGCCA 1 cut(s) 2456
Mly113I GGCGCC 1 cut(s) 2812
MlyI GAGTC 6 cut(s) 296, 868, 1065, 2072, 3050, 3875
MmeI TCCRAC 2 cut(s) 366, 962
Mox20I TGGCCA 1 cut(s) 2456
Mph1103I ATGCAT 3 cut(s) 547, 2179, 2734
MscI TGGCCA 1 cut(s) 2456
MslI CAYNNNNRTG 5 cut(s) 540, 1184, 2456, 2737, 2867
Msp20I TGGCCA 1 cut(s) 2456
MspA1I CMGCKG 3 cut(s) 728, 3487, 4075
MspI CCGG 2 cut(s) 1126, 2083
MspR9I CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
MunI CAATTG 1 cut(s) 1664
Mva1269I GAATGC 2 cut(s) 1738, 2352
MvaI CCWGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
MvnI CGCG 1 cut(s) 3299
MwoI GCNNNNNNNGC 8 cut(s) 1631, 1775, 2500, 3307, 3316, 3770, 3907, 4046
NarI GGCGCC 1 cut(s) 2812
NcoI CCATGG 1 cut(s) 949
NdeI CATATG 2 cut(s) 541, 2868
NlaIV GGNNCC 9 cut(s) 311, 507, 560, 723, 1035, 1798, 2495, 2813, 3558
NmeAIII GCCGAG 1 cut(s) 3061
NmuCI GTSAC 3 cut(s) 2468, 2786, 3128
NsiI ATGCAT 3 cut(s) 547, 2179, 2734
NspI RCATGY 5 cut(s) 1386, 2476, 3917, 3953, 4093
NspV TTCGAA 3 cut(s) 341, 2896, 3016
PagI TCATGA 2 cut(s) 295, 1906
PciI ACATGT 1 cut(s) 2472
PciSI GCTCTTC 2 cut(s) 113, 2604
PctI GAATGC 2 cut(s) 1738, 2352
PflMI CCANNNNNTGG 1 cut(s) 3566
PleI GAGTC 6 cut(s) 295, 868, 1065, 2071, 3049, 3874
PluTI GGCGCC 1 cut(s) 2815
PpsI GAGTC 6 cut(s) 295, 868, 1065, 2071, 3049, 3874
Ppu21I YACGTR 1 cut(s) 857
PpuMI RGGWCCY 2 cut(s) 310, 2935
PscI ACATGT 1 cut(s) 2472
PshBI ATTAAT 1 cut(s) 2250
PsiI TTATAA 1 cut(s) 3330
Psp5II RGGWCCY 2 cut(s) 310, 2935
Psp6I CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2663, 2761, 3134, 3438
PspFI CCCAGC 3 cut(s) 590, 2279, 3394
PspGI CCWGG 9 cut(s) 264, 667, 973, 1049, 2238, 2663, 2761, 3134, 3438
PspN4I GGNNCC 9 cut(s) 311, 507, 560, 723, 1035, 1798, 2495, 2813, 3558
PspOMI GGGCCC 1 cut(s) 558
PspPI GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2935, 3210
PspPPI RGGWCCY 2 cut(s) 310, 2935
PstI CTGCAG 2 cut(s) 1486, 2943
PstNI CAGNNNCTG 2 cut(s) 2938, 3635
PsuI RGATCY 5 cut(s) 649, 721, 1033, 1478, 3280
PvuII CAGCTG 2 cut(s) 728, 3487
RseI CAYNNNNRTG 5 cut(s) 540, 1184, 2456, 2737, 2867
SapI GCTCTTC 2 cut(s) 113, 2604
Sau96I GGNCC 9 cut(s) 310, 558, 559, 1241, 1796, 2085, 2323, 2935, 3210
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 6 cut(s) 296, 868, 1065, 2072, 3050, 3875
ScrFI CCNGG 9 cut(s) 266, 669, 975, 1051, 2240, 2665, 2763, 3136, 3440
SduI GDGCHC 5 cut(s) 47, 562, 834, 971, 2217
SexAI ACCWGGT 1 cut(s) 2761
SfcI CTRYAG 5 cut(s) 930, 1482, 2200, 2877, 2939
SfoI GGCGCC 1 cut(s) 2813
SfuI TTCGAA 3 cut(s) 341, 2896, 3016
SinI GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2935, 3210
SmiMI CAYNNNNRTG 5 cut(s) 540, 1184, 2456, 2737, 2867
SmlI CTYRAG 6 cut(s) 176, 1075, 1748, 3406, 3797, 3863
SmoI CTYRAG 6 cut(s) 176, 1075, 1748, 3406, 3797, 3863
SnaBI TACGTA 1 cut(s) 857
SsiI CCGC 4 cut(s) 240, 1769, 1776, 4075
SspDI GGCGCC 1 cut(s) 2811
SspI AATATT 3 cut(s) 172, 1408, 2524
SspMI CTAG 9 cut(s) 158, 429, 629, 788, 827, 1133, 2349, 3518, 3731
StyD4I CCNGG 9 cut(s) 264, 667, 973, 1049, 2238, 2663, 2761, 3134, 3438
StyI CCWWGG 7 cut(s) 301, 562, 949, 1836, 1954, 2073, 3809
TaaI ACNGT 5 cut(s) 52, 359, 741, 2673, 2840
TaiI ACGT 4 cut(s) 487, 859, 2093, 3595
TatI WGTACW 4 cut(s) 54, 84, 571, 2818
TauI GCSGC 2 cut(s) 1778, 4078
TseFI GTSAC 3 cut(s) 2468, 2786, 3128
Tsp45I GTSAC 3 cut(s) 2468, 2786, 3128
TspGWI ACGGA 1 cut(s) 2315
Van91I CCANNNNNTGG 1 cut(s) 3566
VpaK11BI GGWCC 6 cut(s) 310, 1796, 2085, 2323, 2935, 3210
VspI ATTAAT 1 cut(s) 2250
XagI CCTNNNNNAGG 1 cut(s) 1523
XapI RAATTY 9 cut(s) 244, 617, 703, 925, 1921, 1929, 2953, 3018, 3981
XbaI TCTAGA 1 cut(s) 3517
XceI RCATGY 5 cut(s) 1386, 2476, 3917, 3953, 4093
XmiI GTMKAC 2 cut(s) 1139, 3829
XspI CTAG 9 cut(s) 158, 429, 629, 788, 827, 1133, 2349, 3518, 3731
ZrmI AGTACT 1 cut(s) 86
Zsp2I ATGCAT 3 cut(s) 547, 2179, 2734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.