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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G06210 AT3G06210 AT5G18980 AT5G18980 AT5G18980 |
| fragaria_vesca | FvH4_4g14210 |
| malus_domestica | MD12G1098300.v1.1 MD13G1180800.v1.1 MD16G1181500.v1.1 |
| prunus_persica | Prupe.1G148900_v2.0.a1 |
| pyrus_communis | pycom13g15600 pycom16g15230 |
| rosa_chinensis | RchiOBHm_Chr4g0418921 RchiOBHm_Chr4g0418961 RchiOBHm_Chr4g0418981 |
| rosa_laevigata | RLG00000007835 RLG00000007838 |
| rosa_multiflora | Rmu_sc0000935.1_g000037 Rmu_sc0000935.1_g000062 Rmu_sc0001556.1_g000003 |
| rosa_roxburghii | Rroxscaffold_5G00362050 |
| rosa_rugosa | Rorug04G0155600 Rorug04G0155700 |
| rosa_samantha | Rh4AG216900 Rh4AG217400 Rh4AG217700 Rh4BG212600 Rh4BG212700 Rh4BG213300 Rh4CG228700 Rh4CG229500 Rh4CG229600 Rh4DG215800 Rh4DG216200 Rh4DG216300 |
| rosa_wichuraiana | Rw4G018550 Rw4G018590 Rw4G018600 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2448 |
| AccII | CGCG | 8 cut(s) | 173, 179, 245, 468, 629, 1508, 1836, 2261 |
| AccIII | TCCGGA | 2 cut(s) | 208, 1632 |
| AclWI | GGATC | 3 cut(s) | 1446, 1874, 1975 |
| AcsI | RAATTY | 2 cut(s) | 1249, 2493 |
| AcuI | CTGAAG | 3 cut(s) | 376, 1442, 2072 |
| AcvI | CACGTG | 1 cut(s) | 432 |
| AcyI | GRCGYC | 1 cut(s) | 447 |
| AfaI | GTAC | 5 cut(s) | 612, 652, 1103, 1683, 2434 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 208, 348, 1764 |
| AflIII | ACRYGT | 1 cut(s) | 2013 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 894, 926, 1044, 1076 |
| Alw21I | GWGCWC | 3 cut(s) | 385, 411, 2165 |
| Alw26I | GTCTC | 6 cut(s) | 58, 555, 572, 584, 2200, 2236 |
| AlwI | GGATC | 3 cut(s) | 1446, 1874, 1975 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1094, 1214 |
| Ama87I | CYCGRG | 3 cut(s) | 410, 1721, 1764 |
| Aor13HI | TCCGGA | 2 cut(s) | 208, 1632 |
| AoxI | GGCC | 3 cut(s) | 294, 1408, 1755 |
| ApeKI | GCWGC | 7 cut(s) | 155, 1711, 1871, 1994, 2066, 2069, 2138 |
| ApoI | RAATTY | 2 cut(s) | 1249, 2493 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 86, 118, 2039, 2071 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2040 |
| AspA2I | CCTAGG | 1 cut(s) | 280 |
| AspLEI | GCGC | 1 cut(s) | 470 |
| AspS9I | GGNCC | 3 cut(s) | 294, 435, 1408 |
| AsuC2I | CCSGG | 1 cut(s) | 2029 |
| AsuHPI | GGTGA | 3 cut(s) | 349, 1337, 1600 |
| AsuII | TTCGAA | 1 cut(s) | 18 |
| AsuNHI | GCTAGC | 1 cut(s) | 2482 |
| AvaI | CYCGRG | 3 cut(s) | 410, 1721, 1764 |
| AvaII | GGWCC | 1 cut(s) | 435 |
| AvrII | CCTAGG | 1 cut(s) | 280 |
| BanII | GRGCYC | 2 cut(s) | 411, 2165 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 603, 635 |
| BauI | CACGAG | 3 cut(s) | 404, 1312, 1468 |
| BbrPI | CACGTG | 1 cut(s) | 432 |
| BbsI | GAAGAC | 5 cut(s) | 30, 656, 1469, 1957, 2109 |
| Bbv12I | GWGCWC | 3 cut(s) | 385, 411, 2165 |
| BbvCI | CCTCAGC | 1 cut(s) | 819 |
| BbvI | GCAGC | 7 cut(s) | 167, 1698, 1858, 1981, 2078, 2081, 2150 |
| BccI | CCATC | 7 cut(s) | 368, 627, 983, 994, 2119, 2308, 2353 |
| BceAI | ACGGC | 4 cut(s) | 570, 711, 2415, 2433 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1269, 1303 |
| BciVI | GTATCC | 1 cut(s) | 1105 |
| BcnI | CCSGG | 1 cut(s) | 2029 |
| BcoDI | GTCTC | 6 cut(s) | 58, 555, 572, 584, 2200, 2236 |
| BfaI | CTAG | 7 cut(s) | 281, 897, 1232, 2366, 2390, 2474, 2483 |
| BfmI | CTRYAG | 4 cut(s) | 81, 1580, 1712, 1872 |
| BfuI | GTATCC | 1 cut(s) | 1105 |
| BglII | AGATCT | 2 cut(s) | 1180, 1641 |
| BlnI | CCTAGG | 1 cut(s) | 280 |
| BlpI | GCTNAGC | 2 cut(s) | 484, 2403 |
| Bme1390I | CCNGG | 1 cut(s) | 2029 |
| Bme18I | GGWCC | 1 cut(s) | 435 |
| BmeT110I | CYCGRG | 3 cut(s) | 410, 1721, 1764 |
| BmgT120I | GGNCC | 3 cut(s) | 294, 435, 1408 |
| BmiI | GGNNCC | 4 cut(s) | 277, 340, 677, 2271 |
| BmrFI | CCNGG | 1 cut(s) | 2029 |
| BmsI | GCATC | 2 cut(s) | 1881, 2208 |
| BmtI | GCTAGC | 1 cut(s) | 2486 |
| BpiI | GAAGAC | 5 cut(s) | 30, 656, 1469, 1957, 2109 |
| BpmI | CTGGAG | 2 cut(s) | 662, 1411 |
| Bpu10I | CCTNAGC | 1 cut(s) | 819 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 484, 2403 |
| Bpu14I | TTCGAA | 1 cut(s) | 18 |
| BpuEI | CTTGAG | 6 cut(s) | 272, 905, 1139, 1772, 2399, 2429 |
| BpuMI | CCSGG | 1 cut(s) | 2029 |
| Bsa29I | ATCGAT | 1 cut(s) | 1289 |
| BsaAI | YACGTR | 1 cut(s) | 432 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1130, 1515 |
| BsaHI | GRCGYC | 1 cut(s) | 447 |
| BsaI | GGTCTC | 2 cut(s) | 58, 2236 |
| BsaJI | CCNNGG | 6 cut(s) | 280, 297, 693, 1434, 2028, 2487 |
| BsaWI | WCCGGW | 3 cut(s) | 208, 1632, 2429 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 208, 348, 1764 |
| Bse118I | RCCGGY | 1 cut(s) | 443 |
| Bse1I | ACTGG | 6 cut(s) | 271, 648, 677, 1178, 1789, 2220 |
| Bse3DI | GCAATG | 2 cut(s) | 1287, 1675 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1130, 1515 |
| BseAI | TCCGGA | 2 cut(s) | 208, 1632 |
| BseCI | ATCGAT | 1 cut(s) | 1289 |
| BseDI | CCNNGG | 6 cut(s) | 280, 297, 693, 1434, 2028, 2487 |
| BseGI | GGATG | 6 cut(s) | 1516, 1534, 1684, 1862, 2038, 2316 |
| BseJI | GATNNNNATC | 2 cut(s) | 1130, 1515 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 208, 348, 1764 |
| BseMI | GCAATG | 2 cut(s) | 1287, 1675 |
| BseMII | CTCAG | 6 cut(s) | 498, 618, 810, 1206, 1638, 2394 |
| BseNI | ACTGG | 6 cut(s) | 271, 648, 677, 1178, 1789, 2220 |
| BseXI | GCAGC | 7 cut(s) | 167, 1698, 1858, 1981, 2078, 2081, 2150 |
| BseYI | CCCAGC | 2 cut(s) | 202, 1265 |
| Bsh1236I | CGCG | 8 cut(s) | 173, 179, 245, 468, 629, 1508, 1836, 2261 |
| Bsh1285I | CGRYCG | 3 cut(s) | 177, 542, 1577 |
| BshFI | GGCC | 3 cut(s) | 296, 1410, 1757 |
| BshVI | ATCGAT | 1 cut(s) | 1289 |
| BsiEI | CGRYCG | 3 cut(s) | 177, 542, 1577 |
| BsiHKAI | GWGCWC | 3 cut(s) | 385, 411, 2165 |
| BsiHKCI | CYCGRG | 3 cut(s) | 410, 1721, 1764 |
| BsiSI | CCGG | 5 cut(s) | 209, 444, 1633, 2028, 2430 |
| BslFI | GGGAC | 1 cut(s) | 185 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 208, 348, 1764 |
| BsmAI | GTCTC | 6 cut(s) | 58, 555, 572, 584, 2200, 2236 |
| BsmBI | CGTCTC | 1 cut(s) | 572 |
| BsmFI | GGGAC | 1 cut(s) | 185 |
| BsmI | GAATGC | 2 cut(s) | 39, 2042 |
| BsnI | GGCC | 3 cut(s) | 296, 1410, 1757 |
| Bso31I | GGTCTC | 2 cut(s) | 58, 2236 |
| BsoBI | CYCGRG | 3 cut(s) | 410, 1721, 1764 |
| Bsp119I | TTCGAA | 1 cut(s) | 18 |
| Bsp1286I | GDGCHC | 3 cut(s) | 385, 411, 2165 |
| Bsp13I | TCCGGA | 2 cut(s) | 208, 1632 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 484, 2403 |
| Bsp68I | TCGCGA | 1 cut(s) | 245 |
| BspANI | GGCC | 3 cut(s) | 296, 1410, 1757 |
| BspCNI | CTCAG | 6 cut(s) | 497, 617, 811, 1207, 1639, 2395 |
| BspDI | ATCGAT | 1 cut(s) | 1289 |
| BspEI | TCCGGA | 2 cut(s) | 208, 1632 |
| BspFNI | CGCG | 8 cut(s) | 173, 179, 245, 468, 629, 1508, 1836, 2261 |
| BspHI | TCATGA | 3 cut(s) | 1441, 2044, 2157 |
| BspLI | GGNNCC | 4 cut(s) | 277, 340, 677, 2271 |
| BspMAI | CTGCAG | 2 cut(s) | 1716, 1876 |
| BspOI | GCTAGC | 1 cut(s) | 2486 |
| BspPI | GGATC | 3 cut(s) | 1446, 1874, 1975 |
| BspT104I | TTCGAA | 1 cut(s) | 18 |
| BspTNI | GGTCTC | 2 cut(s) | 58, 2236 |
| BsrDI | GCAATG | 2 cut(s) | 1287, 1675 |
| BsrFI | RCCGGY | 1 cut(s) | 443 |
| BsrI | ACTGG | 6 cut(s) | 271, 648, 677, 1178, 1789, 2220 |
| BssAI | RCCGGY | 1 cut(s) | 443 |
| BssECI | CCNNGG | 6 cut(s) | 280, 297, 693, 1434, 2028, 2487 |
| BssNI | GRCGYC | 1 cut(s) | 447 |
| BssSI | CACGAG | 3 cut(s) | 404, 1312, 1468 |
| BssT1I | CCWWGG | 3 cut(s) | 280, 1434, 2487 |
| Bst2BI | CACGAG | 3 cut(s) | 404, 1312, 1468 |
| Bst6I | CTCTTC | 3 cut(s) | 621, 1048, 1188 |
| BstACI | GRCGYC | 1 cut(s) | 447 |
| BstBAI | YACGTR | 1 cut(s) | 432 |
| BstBI | TTCGAA | 1 cut(s) | 18 |
| BstC8I | GCNNGC | 7 cut(s) | 445, 810, 1237, 1603, 2259, 2301, 2484 |
| BstDSI | CCRYGG | 2 cut(s) | 297, 693 |
| BstF5I | GGATG | 6 cut(s) | 1516, 1534, 1684, 1862, 2038, 2316 |
| BstFNI | CGCG | 8 cut(s) | 173, 179, 245, 468, 629, 1508, 1836, 2261 |
| BstHHI | GCGC | 1 cut(s) | 470 |
| BstMAI | GTCTC | 6 cut(s) | 58, 555, 572, 584, 2200, 2236 |
| BstMCI | CGRYCG | 3 cut(s) | 177, 542, 1577 |
| BstNSI | RCATGY | 1 cut(s) | 2017 |
| BstSCI | CCNGG | 1 cut(s) | 2027 |
| BstSFI | CTRYAG | 4 cut(s) | 81, 1580, 1712, 1872 |
| BstUI | CGCG | 8 cut(s) | 173, 179, 245, 468, 629, 1508, 1836, 2261 |
| BstV1I | GCAGC | 7 cut(s) | 167, 1698, 1858, 1981, 2078, 2081, 2150 |
| BstV2I | GAAGAC | 5 cut(s) | 30, 656, 1469, 1957, 2109 |
| BstX2I | RGATCY | 2 cut(s) | 1180, 1641 |
| BstYI | RGATCY | 2 cut(s) | 1180, 1641 |
| Bsu15I | ATCGAT | 1 cut(s) | 1289 |
| BsuI | GTATCC | 1 cut(s) | 1105 |
| BsuRI | GGCC | 3 cut(s) | 296, 1410, 1757 |
| BsuTUI | ATCGAT | 1 cut(s) | 1289 |
| BtgI | CCRYGG | 2 cut(s) | 297, 693 |
| BtgZI | GCGATG | 1 cut(s) | 1904 |
| BtsCI | GGATG | 6 cut(s) | 1516, 1534, 1684, 1862, 2038, 2316 |
| BtsIMutI | CAGTG | 6 cut(s) | 264, 523, 660, 874, 1095, 1565 |
| BtuMI | TCGCGA | 1 cut(s) | 245 |
| Cac8I | GCNNGC | 7 cut(s) | 445, 810, 1237, 1603, 2259, 2301, 2484 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1094, 1214 |
| CciI | TCATGA | 3 cut(s) | 1441, 2044, 2157 |
| CfoI | GCGC | 1 cut(s) | 470 |
| Cfr10I | RCCGGY | 1 cut(s) | 443 |
| Cfr13I | GGNCC | 3 cut(s) | 294, 435, 1408 |
| ClaI | ATCGAT | 1 cut(s) | 1289 |
| CseI | GACGC | 3 cut(s) | 161, 436, 556 |
| Csp6I | GTAC | 5 cut(s) | 611, 651, 1102, 1682, 2433 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 413, 448 |
| CviAII | CATG | 8 cut(s) | 594, 1364, 1442, 1925, 2014, 2045, 2158, 2188 |
| CviQI | GTAC | 5 cut(s) | 611, 651, 1102, 1682, 2433 |
| DraI | TTTAAA | 1 cut(s) | 1822 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2448 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2448 |
| Eam1104I | CTCTTC | 3 cut(s) | 621, 1048, 1188 |
| EarI | CTCTTC | 3 cut(s) | 621, 1048, 1188 |
| EciI | GGCGGA | 1 cut(s) | 1794 |
| Ecl136II | GAGCTC | 2 cut(s) | 409, 2163 |
| Eco130I | CCWWGG | 3 cut(s) | 280, 1434, 2487 |
| Eco24I | GRGCYC | 2 cut(s) | 411, 2165 |
| Eco31I | GGTCTC | 2 cut(s) | 58, 2236 |
| Eco47I | GGWCC | 1 cut(s) | 435 |
| Eco53kI | GAGCTC | 2 cut(s) | 409, 2163 |
| Eco57I | CTGAAG | 3 cut(s) | 376, 1442, 2072 |
| Eco72I | CACGTG | 1 cut(s) | 432 |
| Eco88I | CYCGRG | 3 cut(s) | 410, 1721, 1764 |
| EcoICRI | GAGCTC | 2 cut(s) | 409, 2163 |
| EcoRI | GAATTC | 1 cut(s) | 1249 |
| EcoT14I | CCWWGG | 3 cut(s) | 280, 1434, 2487 |
| EcoT38I | GRGCYC | 2 cut(s) | 411, 2165 |
| ErhI | CCWWGG | 3 cut(s) | 280, 1434, 2487 |
| Esp3I | CGTCTC | 1 cut(s) | 572 |
| FaeI | CATG | 8 cut(s) | 597, 1367, 1445, 1928, 2017, 2048, 2161, 2191 |
| FaqI | GGGAC | 1 cut(s) | 185 |
| FatI | CATG | 8 cut(s) | 593, 1363, 1441, 1924, 2013, 2044, 2157, 2187 |
| FauI | CCCGC | 1 cut(s) | 2266 |
| FokI | GGATG | 6 cut(s) | 1523, 1541, 1691, 1869, 2045, 2323 |
| FriOI | GRGCYC | 2 cut(s) | 411, 2165 |
| FspBI | CTAG | 7 cut(s) | 281, 897, 1232, 2366, 2390, 2474, 2483 |
| GlaI | GCGC | 1 cut(s) | 469 |
| GsaI | CCCAGC | 2 cut(s) | 206, 1269 |
| GsuI | CTGGAG | 2 cut(s) | 662, 1411 |
| HaeIII | GGCC | 3 cut(s) | 296, 1410, 1757 |
| HapII | CCGG | 5 cut(s) | 209, 444, 1633, 2028, 2430 |
| HgaI | GACGC | 3 cut(s) | 161, 436, 556 |
| HhaI | GCGC | 1 cut(s) | 470 |
| Hin1I | GRCGYC | 1 cut(s) | 447 |
| Hin1II | CATG | 8 cut(s) | 597, 1367, 1445, 1928, 2017, 2048, 2161, 2191 |
| Hin6I | GCGC | 1 cut(s) | 468 |
| HinP1I | GCGC | 1 cut(s) | 468 |
| HindIII | AAGCTT | 1 cut(s) | 722 |
| HpaII | CCGG | 5 cut(s) | 209, 444, 1633, 2028, 2430 |
| HphI | GGTGA | 3 cut(s) | 349, 1337, 1600 |
| Hpy166II | GTNNAC | 4 cut(s) | 47, 435, 751, 1990 |
| Hpy8I | GTNNAC | 4 cut(s) | 47, 435, 751, 1990 |
| Hpy99I | CGWCG | 6 cut(s) | 338, 443, 452, 556, 572, 586 |
| HpyAV | CCTTC | 5 cut(s) | 371, 1421, 1838, 1986, 2377 |
| HpyCH4IV | ACGT | 3 cut(s) | 431, 584, 609 |
| HpyCH4V | TGCA | 5 cut(s) | 1186, 1280, 1714, 1748, 1874 |
| HpySE526I | ACGT | 3 cut(s) | 431, 584, 609 |
| Hsp92I | GRCGYC | 1 cut(s) | 447 |
| Hsp92II | CATG | 8 cut(s) | 597, 1367, 1445, 1928, 2017, 2048, 2161, 2191 |
| HspAI | GCGC | 1 cut(s) | 468 |
| Kpn2I | TCCGGA | 2 cut(s) | 208, 1632 |
| KroI | GCCGGC | 1 cut(s) | 443 |
| KroNI | GCCGGC | 1 cut(s) | 445 |
| LmnI | GCTCC | 5 cut(s) | 58, 211, 681, 1024, 1336 |
| Lsp1109I | GCAGC | 7 cut(s) | 167, 1698, 1858, 1981, 2078, 2081, 2150 |
| LweI | GCATC | 2 cut(s) | 1881, 2208 |
| MaeI | CTAG | 7 cut(s) | 281, 897, 1232, 2366, 2390, 2474, 2483 |
| MaeII | ACGT | 3 cut(s) | 431, 584, 609 |
| MaeIII | GTNAC | 4 cut(s) | 401, 892, 1090, 1551 |
| MflI | RGATCY | 2 cut(s) | 1180, 1641 |
| MhlI | GDGCHC | 3 cut(s) | 385, 411, 2165 |
| MlyI | GAGTC | 2 cut(s) | 667, 1269 |
| MmeI | TCCRAC | 4 cut(s) | 430, 1881, 2310, 2419 |
| MreI | CGCCGGCG | 1 cut(s) | 443 |
| MroI | TCCGGA | 2 cut(s) | 208, 1632 |
| MroNI | GCCGGC | 1 cut(s) | 443 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2040 |
| MseI | TTAA | 3 cut(s) | 1821, 2061, 2265 |
| MslI | CAYNNNNRTG | 2 cut(s) | 738, 1509 |
| MspA1I | CMGCKG | 3 cut(s) | 138, 1214, 1585 |
| MspI | CCGG | 5 cut(s) | 209, 444, 1633, 2028, 2430 |
| MspR9I | CCNGG | 1 cut(s) | 2029 |
| Mva1269I | GAATGC | 2 cut(s) | 39, 2042 |
| MvnI | CGCG | 8 cut(s) | 173, 179, 245, 468, 629, 1508, 1836, 2261 |
| NaeI | GCCGGC | 1 cut(s) | 445 |
| NciI | CCSGG | 1 cut(s) | 2029 |
| NgoMIV | GCCGGC | 1 cut(s) | 443 |
| NheI | GCTAGC | 1 cut(s) | 2482 |
| NlaIII | CATG | 8 cut(s) | 597, 1367, 1445, 1928, 2017, 2048, 2161, 2191 |
| NlaIV | GGNNCC | 4 cut(s) | 277, 340, 677, 2271 |
| NmeAIII | GCCGAG | 2 cut(s) | 291, 1440 |
| NmuCI | GTSAC | 4 cut(s) | 401, 892, 1090, 1551 |
| NruI | TCGCGA | 1 cut(s) | 245 |
| NspI | RCATGY | 1 cut(s) | 2017 |
| NspV | TTCGAA | 1 cut(s) | 18 |
| PaeR7I | CTCGAG | 1 cut(s) | 410 |
| PagI | TCATGA | 3 cut(s) | 1441, 2044, 2157 |
| PciI | ACATGT | 1 cut(s) | 2013 |
| PcsI | WCGNNNNNNNCGW | 7 cut(s) | 24, 40, 129, 339, 472, 548, 1295 |
| PctI | GAATGC | 2 cut(s) | 39, 2042 |
| PdiI | GCCGGC | 1 cut(s) | 445 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2040 |
| PflFI | GACNNNGTC | 1 cut(s) | 566 |
| Ple19I | CGATCG | 1 cut(s) | 542 |
| PleI | GAGTC | 2 cut(s) | 666, 1268 |
| PmaCI | CACGTG | 1 cut(s) | 432 |
| PmlI | CACGTG | 1 cut(s) | 432 |
| PpsI | GAGTC | 2 cut(s) | 666, 1268 |
| Ppu21I | YACGTR | 1 cut(s) | 432 |
| PscI | ACATGT | 1 cut(s) | 2013 |
| Psp124BI | GAGCTC | 2 cut(s) | 411, 2165 |
| PspCI | CACGTG | 1 cut(s) | 432 |
| PspFI | CCCAGC | 2 cut(s) | 202, 1265 |
| PspN4I | GGNNCC | 4 cut(s) | 277, 340, 677, 2271 |
| PspPI | GGNCC | 3 cut(s) | 294, 435, 1408 |
| PspXI | VCTCGAGB | 1 cut(s) | 410 |
| PstI | CTGCAG | 2 cut(s) | 1716, 1876 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1094, 1214 |
| PsuI | RGATCY | 2 cut(s) | 1180, 1641 |
| PsyI | GACNNNGTC | 1 cut(s) | 566 |
| PvuI | CGATCG | 1 cut(s) | 542 |
| PvuII | CAGCTG | 1 cut(s) | 1585 |
| RruI | TCGCGA | 1 cut(s) | 245 |
| RsaI | GTAC | 5 cut(s) | 612, 652, 1103, 1683, 2434 |
| RsaNI | GTAC | 5 cut(s) | 611, 651, 1102, 1682, 2433 |
| RseI | CAYNNNNRTG | 2 cut(s) | 738, 1509 |
| SacI | GAGCTC | 2 cut(s) | 411, 2165 |
| SaqAI | TTAA | 3 cut(s) | 1821, 2061, 2265 |
| Sau96I | GGNCC | 3 cut(s) | 294, 435, 1408 |
| SchI | GAGTC | 2 cut(s) | 667, 1269 |
| ScrFI | CCNGG | 1 cut(s) | 2029 |
| SduI | GDGCHC | 3 cut(s) | 385, 411, 2165 |
| SfaNI | GCATC | 2 cut(s) | 1881, 2208 |
| SfcI | CTRYAG | 4 cut(s) | 81, 1580, 1712, 1872 |
| Sfr274I | CTCGAG | 1 cut(s) | 410 |
| SfuI | TTCGAA | 1 cut(s) | 18 |
| SgrAI | CRCCGGYG | 1 cut(s) | 443 |
| SinI | GGWCC | 1 cut(s) | 435 |
| SlaI | CTCGAG | 1 cut(s) | 410 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 738, 1509 |
| SmlI | CTYRAG | 7 cut(s) | 251, 410, 884, 1118, 1751, 2378, 2408 |
| SmoI | CTYRAG | 7 cut(s) | 251, 410, 884, 1118, 1751, 2378, 2408 |
| SspMI | CTAG | 7 cut(s) | 281, 897, 1232, 2366, 2390, 2474, 2483 |
| SstI | GAGCTC | 2 cut(s) | 411, 2165 |
| StyD4I | CCNGG | 1 cut(s) | 2027 |
| StyI | CCWWGG | 3 cut(s) | 280, 1434, 2487 |
| TaiI | ACGT | 3 cut(s) | 434, 587, 612 |
| TatI | WGTACW | 2 cut(s) | 650, 1681 |
| TauI | GCSGC | 3 cut(s) | 141, 318, 1214 |
| Tru1I | TTAA | 3 cut(s) | 1821, 2061, 2265 |
| Tru9I | TTAA | 3 cut(s) | 1821, 2061, 2265 |
| TscAI | CASTG | 6 cut(s) | 271, 523, 660, 874, 1095, 1572 |
| TseFI | GTSAC | 4 cut(s) | 401, 892, 1090, 1551 |
| TseI | GCWGC | 7 cut(s) | 155, 1711, 1871, 1994, 2066, 2069, 2138 |
| Tsp45I | GTSAC | 4 cut(s) | 401, 892, 1090, 1551 |
| TspGWI | ACGGA | 2 cut(s) | 779, 1973 |
| TspRI | CASTG | 6 cut(s) | 271, 523, 660, 874, 1095, 1572 |
| Tth111I | GACNNNGTC | 1 cut(s) | 566 |
| VpaK11BI | GGWCC | 1 cut(s) | 435 |
| XapI | RAATTY | 2 cut(s) | 1249, 2493 |
| XbaI | TCTAGA | 2 cut(s) | 2365, 2389 |
| XceI | RCATGY | 1 cut(s) | 2017 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 630 |
| XhoI | CTCGAG | 1 cut(s) | 410 |
| XmaJI | CCTAGG | 1 cut(s) | 280 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2040 |
| XspI | CTAG | 7 cut(s) | 281, 897, 1232, 2366, 2390, 2474, 2483 |