Rorug04G0155600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003020
Physical Location & Seq
Reverse (-)
27217868 .. 27221309
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0155600.1

Sequence Viewer

Length: 747 bp
ATGACGATAGCAGCAGGGATCGGTTACGCCTTGTTAGCACTCGGCCCGTCTCTCTCCCTTTTCATCTCTGTTATCTCCAAAAAGCCCTTCCTGGTTCTCACCGTCCTTTCCAGTACATTGTTATGGCTTGTAAGTCTCATCATGTTGTCGGGACTGTGGAGGCCATTTCTGCCTCTGAAATCATCCGCATGGTGGCCCTATGCCCTCCTCATATTCACCTCAGTTCTCTTTCAAGAAGCCCTTCGGATTCTTTTCTGGAAGGTCTATAAGAAGTTGGAAGATACATTGGATGCATTTGCTGATAGAGTATCTAAACCACGCCTGTATCTGACTGATAAGATGCAAATTGCTCTGGCTGGTGGTTTGGGTCATGGCGTGGCACATGCTGTGTTCTTTTGTCTCAGTCTCTTAACACCAGCATTTGGTCCAGGAACTTTTTTTGTCGACAGGTGCTCAAAGATACCCTTTTTTCTTCTTTCTGCTATCATTGCCCTTGCATTTGTTACAATTCACACATTTTCGATGGTCATTGCATTTAATGGATATGCAGAAGGCAATAAAGTGGACCAGATTTTTGTACCTTTAGTTCATCTTGTTGCAGGGATGGTGACACTAGTTAATTTTGCATCTGGGGGATGTATTATCGGTATTCCTCTCCTCTACTTTGTCGCTGTGTTAACCTTATTGCATTGTGGGAAGATGGTGTGGAGAAGGTTAACAGAGAACAGACACAGACCGGTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.43

Weight (kDa)

9.69

Isoelectric Point (pI)

26.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aph-1 PF06105 5 - 238 5.9e-75 Aph-1 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 1 cut(s) 444
AciI CCGC 1 cut(s) 186
AclWI GGATC 1 cut(s) 26
AfaI GTAC 2 cut(s) 115, 579
AfiI CCNNNNNNNGG 2 cut(s) 192, 422
AgeI ACCGGT 1 cut(s) 736
AgsI TTSAA 1 cut(s) 233
AhlI ACTAGT 1 cut(s) 613
AjnI CCWGG 2 cut(s) 90, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 4 cut(s) 54, 140, 404, 410
AlwI GGATC 1 cut(s) 26
AoxI GGCC 3 cut(s) 43, 161, 194
ApeKI GCWGC 1 cut(s) 11
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
AsiGI ACCGGT 1 cut(s) 736
Asp700I GAANNNNTTC 1 cut(s) 240
AspS9I GGNCC 4 cut(s) 44, 195, 425, 565
AsuHPI GGTGA 3 cut(s) 91, 208, 619
AvaII GGWCC 2 cut(s) 425, 565
BaeI ACNNNNGTAYC 2 cut(s) 308, 341
Bbv12I GWGCWC 1 cut(s) 455
BbvI GCAGC 1 cut(s) 23
BccI CCATC 3 cut(s) 517, 598, 694
BciT130I CCWGG 2 cut(s) 92, 429
BcoDI GTCTC 4 cut(s) 54, 140, 404, 410
BcuI ACTAGT 1 cut(s) 613
BfaI CTAG 1 cut(s) 614
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 2 cut(s) 92, 429
Bme18I GGWCC 2 cut(s) 425, 565
BmgT120I GGNCC 4 cut(s) 44, 195, 425, 565
BmrFI CCNGG 2 cut(s) 92, 429
BmsI GCATC 3 cut(s) 280, 330, 635
BsaWI WCCGGW 1 cut(s) 736
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 422
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 1 cut(s) 111
Bse3DI GCAATG 2 cut(s) 486, 528
BseBI CCWGG 2 cut(s) 92, 429
BseGI GGATG 4 cut(s) 182, 295, 609, 641
BseLI CCNNNNNNNGG 2 cut(s) 192, 422
BseMI GCAATG 2 cut(s) 486, 528
BseMII CTCAG 2 cut(s) 234, 415
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 2 cut(s) 197, 647
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 3 cut(s) 45, 163, 196
BshTI ACCGGT 1 cut(s) 736
BsiHKAI GWGCWC 1 cut(s) 455
BsiSI CCGG 1 cut(s) 737
BslFI GGGAC 1 cut(s) 165
BslI CCNNNNNNNGG 2 cut(s) 192, 422
BsmAI GTCTC 4 cut(s) 54, 140, 404, 410
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 165
BsnI GGCC 3 cut(s) 45, 163, 196
Bsp1286I GDGCHC 1 cut(s) 455
Bsp143I GATC 1 cut(s) 18
BspACI CCGC 1 cut(s) 186
BspANI GGCC 3 cut(s) 45, 163, 196
BspCNI CTCAG 2 cut(s) 233, 414
BspPI GGATC 1 cut(s) 26
BsrDI GCAATG 2 cut(s) 486, 528
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 1 cut(s) 111
BssAI RCCGGY 1 cut(s) 736
BssMI GATC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 92, 429
Bst4CI ACNGT 2 cut(s) 103, 156
BstDEI CTNAG 2 cut(s) 220, 401
BstF5I GGATG 4 cut(s) 182, 295, 609, 641
BstKTI GATC 1 cut(s) 21
BstMAI GTCTC 4 cut(s) 54, 140, 404, 410
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 3 cut(s) 35, 169, 488
BstNI CCWGG 2 cut(s) 92, 429
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 2 cut(s) 90, 427
BstV1I GCAGC 1 cut(s) 23
BsuRI GGCC 3 cut(s) 45, 163, 196
BtsCI GGATG 4 cut(s) 182, 295, 609, 641
Cfr10I RCCGGY 1 cut(s) 736
Cfr13I GGNCC 4 cut(s) 44, 195, 425, 565
Csp6I GTAC 2 cut(s) 114, 578
CspAI ACCGGT 1 cut(s) 736
CviAII CATG 5 cut(s) 142, 189, 371, 383, 742
CviJI RGCY 7 cut(s) 45, 85, 127, 163, 196, 239, 356
CviKI_1 RGCY 7 cut(s) 45, 85, 127, 163, 196, 239, 356
CviQI GTAC 2 cut(s) 114, 578
DdeI CTNAG 2 cut(s) 220, 401
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 425, 565
EcoRII CCWGG 2 cut(s) 90, 427
EcoT22I ATGCAT 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 5 cut(s) 145, 192, 374, 386, 745
FalI AAGNNNNNCTT 4 cut(s) 225, 257, 449, 481
FaqI GGGAC 1 cut(s) 165
FatI CATG 5 cut(s) 141, 188, 370, 382, 741
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 4 cut(s) 169, 302, 616, 648
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 614
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 3 cut(s) 45, 163, 196
HapII CCGG 1 cut(s) 737
Hin1II CATG 5 cut(s) 145, 192, 374, 386, 745
HincII GTYRAC 3 cut(s) 445, 678, 717
HindII GTYRAC 3 cut(s) 445, 678, 717
HinfI GANTC 1 cut(s) 247
HpaI GTTAAC 2 cut(s) 678, 717
HpaII CCGG 1 cut(s) 737
HphI GGTGA 3 cut(s) 91, 208, 619
Hpy166II GTNNAC 4 cut(s) 445, 565, 678, 717
Hpy188I TCNGA 3 cut(s) 177, 246, 330
Hpy188III TCNNGA 3 cut(s) 150, 233, 256
Hpy8I GTNNAC 4 cut(s) 445, 565, 678, 717
HpyAV CCTTC 5 cut(s) 97, 251, 253, 545, 705
HpyCH4III ACNGT 2 cut(s) 103, 156
HpyCH4V TGCA 8 cut(s) 293, 343, 497, 533, 548, 599, 626, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 169, 488
HpyF3I CTNAG 2 cut(s) 220, 401
Hsp92II CATG 5 cut(s) 145, 192, 374, 386, 745
KspAI GTTAAC 2 cut(s) 678, 717
Kzo9I GATC 1 cut(s) 18
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 3 cut(s) 280, 330, 635
MaeI CTAG 1 cut(s) 614
MaeIII GTNAC 3 cut(s) 23, 502, 607
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MboII GAAGA 3 cut(s) 290, 464, 709
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 3 cut(s) 345, 507, 619
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 7 cut(s) 153, 183, 215, 218, 229, 663, 668
Mph1103I ATGCAT 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 5 cut(s) 410, 537, 618, 677, 716
MslI CAYNNNNRTG 2 cut(s) 121, 187
MspI CCGG 1 cut(s) 737
MspR9I CCNGG 2 cut(s) 92, 429
MvaI CCWGG 2 cut(s) 92, 429
MwoI GCNNNNNNNGC 3 cut(s) 35, 169, 488
NdeII GATC 1 cut(s) 18
NlaIII CATG 5 cut(s) 145, 192, 374, 386, 745
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 1 cut(s) 607
NsiI ATGCAT 1 cut(s) 295
NspI RCATGY 1 cut(s) 386
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 427
PinAI ACCGGT 1 cut(s) 736
PkrI GCNGC 1 cut(s) 13
Psp6I CCWGG 2 cut(s) 90, 427
PspGI CCWGG 2 cut(s) 90, 427
PspPI GGNCC 4 cut(s) 44, 195, 425, 565
PsrI GAACNNNNNNTAC 2 cut(s) 570, 602
RsaI GTAC 2 cut(s) 115, 579
RsaNI GTAC 2 cut(s) 114, 578
RseI CAYNNNNRTG 2 cut(s) 121, 187
SalI GTCGAC 1 cut(s) 443
SaqAI TTAA 5 cut(s) 410, 537, 618, 677, 716
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 18
Sau96I GGNCC 4 cut(s) 44, 195, 425, 565
ScrFI CCNGG 2 cut(s) 92, 429
SduI GDGCHC 1 cut(s) 455
SetI ASST 6 cut(s) 221, 264, 452, 583, 683, 716
SfaNI GCATC 3 cut(s) 280, 330, 635
SinI GGWCC 2 cut(s) 425, 565
SmiMI CAYNNNNRTG 2 cut(s) 121, 187
SpeI ACTAGT 1 cut(s) 613
Sse9I AATT 3 cut(s) 345, 507, 619
SsiI CCGC 1 cut(s) 186
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 2 cut(s) 90, 427
TaaI ACNGT 2 cut(s) 103, 156
TaqI TCGA 2 cut(s) 444, 521
TasI AATT 3 cut(s) 345, 507, 619
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 5 cut(s) 410, 537, 618, 677, 716
Tru9I TTAA 5 cut(s) 410, 537, 618, 677, 716
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 2 cut(s) 52, 578
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 425, 565
XceI RCATGY 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.