Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014912

Physical Location & Seq

Genomic Coordinates Reverse (-)

6682420 .. 6684744

Gene Structure 2325 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G19770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGCGAGAAATCATAAGCATTCACATCGGACAAGCTGGGATCCAGGTCGGAAATTCGTGTTGGGAGCTTTACTGTCTCGAACATGGAATCCAGCCGGACGGAATGATGCCGAGTGATACTACAGTTGGTGTTGCACACGATGCGTTCAATACTTTCTTTAGCGAGACTGGTGCTGGGAAGCATGTCCCTAGGGCTGTCTTCGTTGATCTCGAGCCTACCGTTATCGACGAAGTTCGTACTGGTACTTACCGTCAACTTTTCCATCCAGAGCAGCTCATTTCTGGGAAAGAAGATGCTGCTAACAACTTCGCTAGAGGACATTACACTGTTGGGAAGGAAATTGTGGATCTATGTCTTGACCGTGTGAGGAAACTTGCCGACAACTGTACTGGCTTACAAGGGTTTTTGGTGTTCAATGCTGTTGGTGGTGGAACTGGTTCTGGATTGGGTTCTCTGTTGCTAGAGCGTTTGTCTGTAGATTATGGAAAGAAGTCTAAGCTTGGTTTTACCATATACCCTTCTCCTCAGGTTTCTACTGCTGTTGTAGAACCTTACAACAGTGTTCTCTCAACGCATTCCCTTCTTGAACATACGGATGTAGCTGTCCTCTTGGATAACGAAGCCATCTATGACATTTGCCGCAGATCCCTAGATATCGAGAGGCCAACCTACACAAACTTGAACAGGTTGATATCACAGATCATTTCATCCTTGACAACATCTTTGAGGTTTGATGGTGCCATCAACGTGGATATCACTGAGTTCCAGACCAATCTTGTCCCATATCCCCGTATCCATTTCATGCTGTCATCTTATGCACCAGTCATCTCAGCCGCCAAGGCTTACCACGAGCAGCTATCAGTCCCTGAGATCACCAATGCCGTTTTTGAGCCAGCTAGCATGATGGCAAAGTGTGACCCGAGACACGGAAAGTACATGGCCTGTTGTTTGATGTACCGAGGAGATGTTGTTCCCAAAGATGTTAATGCTGCTGTTGGCACCATCAAGACAAAGAGGACTGTTCAGTTTGTTGACTGGTGCCCAACTGGGTTCAAATGTGGAATCAACTACCAACCTCCAACAGTTGTTCCAGGTGGTGACCTCGCTAAGGTTCAGAGAGCTGTATGTATGATCAGTAACAACACAGCAGTTGCAGAGGTGTTCTCACGGATCGACCACAAGTTTGATCTCATGTATGCGAAGAGGGCATTTGTCCACTGGTACGTTGGTGAAGGAATGGAGGAAGGTGAATTCTCTGAGGCACGTGAAGACTTGGCCGCACTGGAGAAAGATTACGAAGAAGTTGGTGCTGAAGGTGGAGACGATGAAGAAGATGAAGGTGAAGACTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

49.65

Weight (kDa)

4.95

Isoelectric Point (pI)

36.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 213	1e-62	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	263 - 392	3.4e-51	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014912)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19770 AT5G19780
fragaria_vesca	FvH4_3g45490
malus_domestica	MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica	Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis	pycom03g00450 pycom11g00410
rosa_chinensis	RchiOBHm_Chr5g0083281
rosa_laevigata	RLG00000037026
rosa_multiflora	Rmu_sc0011792.1_g000008
rosa_roxburghii	Rroxscaffold_1G00001210
rosa_rugosa	Rorug05G0484000
rosa_samantha	Rh5BG560700
rosa_wichuraiana	Rw5G049950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	737, 998, 1038
AciI	CCGC	3 cut(s)	640, 834, 1278
AclWI	GGATC	5 cut(s)	34, 47, 354, 639, 1178
AcoI	YGGCCR	1 cut(s)	1275
AcsI	RAATTY	2 cut(s)	52, 1250
AcuI	CTGAAG	1 cut(s)	1332
AcvI	CACGTG	1 cut(s)	1265
AfaI	GTAC	6 cut(s)	238, 244, 388, 935, 956, 1223
AfiI	CCNNNNNNNGG	2 cut(s)	926, 1108
AgsI	TTSAA	5 cut(s)	148, 415, 587, 682, 1054
AjnI	CCWGG	2 cut(s)	42, 1090
AjuI	GAANNNNNNNTTGG	2 cut(s)	43, 75
AloI	GAACNNNNNNTCC	4 cut(s)	72, 104, 954, 986
AluBI	AGCT	8 cut(s)	35, 67, 274, 499, 602, 856, 896, 1121
AluI	AGCT	8 cut(s)	35, 67, 274, 499, 602, 856, 896, 1121
Alw26I	GTCTC	4 cut(s)	80, 158, 916, 1314
AlwI	GGATC	5 cut(s)	34, 47, 354, 639, 1178
AlwNI	CAGNNNCTG	1 cut(s)	866
Ama87I	CYCGRG	2 cut(s)	209, 919
AoxI	GGCC	3 cut(s)	662, 939, 1275
ApeKI	GCWGC	4 cut(s)	271, 296, 853, 989
ApoI	RAATTY	2 cut(s)	52, 1250
ArsI	GACNNNNNNTTYG	4 cut(s)	706, 738, 1166, 1198
Asp700I	GAANNNNTTC	1 cut(s)	436
AspA2I	CCTAGG	1 cut(s)	188
AsuHPI	GGTGA	5 cut(s)	865, 1109, 1241, 1259, 1352
AsuNHI	GCTAGC	1 cut(s)	896
AvaI	CYCGRG	2 cut(s)	209, 919
AvrII	CCTAGG	1 cut(s)	188
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	1 cut(s)	1043
BaeI	ACNNNNGTAYC	4 cut(s)	108, 141, 1213, 1246
BamHI	GGATCC	1 cut(s)	39
BanI	GGYRCC	3 cut(s)	737, 998, 1038
BauI	CACGAG	1 cut(s)	848
BbrPI	CACGTG	1 cut(s)	1265
BbsI	GAAGAC	2 cut(s)	190, 1275
BbvI	GCAGC	4 cut(s)	283, 283, 865, 976
BccI	CCATC	6 cut(s)	270, 632, 728, 749, 898, 1010
BceAI	ACGGC	1 cut(s)	866
BcgI	CGANNNNNNTGC	4 cut(s)	7, 41, 152, 186
BciT130I	CCWGG	2 cut(s)	44, 1092
BciVI	GTATCC	1 cut(s)	803
BclI	TGATCA	1 cut(s)	1131
BcoDI	GTCTC	4 cut(s)	80, 158, 916, 1314
BfaI	CTAG	5 cut(s)	189, 312, 461, 650, 897
BfmI	CTRYAG	2 cut(s)	120, 474
BfuI	GTATCC	1 cut(s)	803
BglI	GCCNNNNNGGC	1 cut(s)	839
BisI	GCNGC	7 cut(s)	272, 297, 640, 834, 854, 990, 1278
BlnI	CCTAGG	1 cut(s)	188
BlsI	GCNGC	7 cut(s)	273, 298, 641, 835, 855, 991, 1279
Bme1390I	CCNGG	2 cut(s)	44, 1092
BmeT110I	CYCGRG	2 cut(s)	209, 919
BmiI	GGNNCC	4 cut(s)	41, 739, 1000, 1040
BmrFI	CCNGG	2 cut(s)	44, 1092
BmrI	ACTGGG	1 cut(s)	1056
BmsI	GCATC	3 cut(s)	96, 130, 283
BmtI	GCTAGC	1 cut(s)	900
BmuI	ACTGGG	1 cut(s)	1056
BpiI	GAAGAC	2 cut(s)	190, 1275
BplI	GAGNNNNNCTC	2 cut(s)	1148, 1180
BpmI	CTGGAG	1 cut(s)	1304
Bpu10I	CCTNAGC	1 cut(s)	1107
BsaAI	YACGTR	1 cut(s)	1265
BsaJI	CCNNGG	3 cut(s)	188, 837, 958
BsaXI	ACNNNNNCTCC	2 cut(s)	954, 984
Bsc4I	CCNNNNNNNGG	2 cut(s)	926, 1108
Bse1I	ACTGG	9 cut(s)	172, 244, 394, 439, 821, 1040, 1051, 1223, 1287
Bse21I	CCTNAGG	1 cut(s)	525
BseBI	CCWGG	2 cut(s)	44, 1092
BseDI	CCNNGG	3 cut(s)	188, 837, 958
BseGI	GGATG	3 cut(s)	262, 601, 707
BseLI	CCNNNNNNNGG	2 cut(s)	926, 1108
BseMII	CTCAG	5 cut(s)	539, 750, 843, 858, 1248
BseNI	ACTGG	9 cut(s)	172, 244, 394, 439, 821, 1040, 1051, 1223, 1287
BseRI	GAGGAG	2 cut(s)	513, 975
BseSI	GKGCMC	1 cut(s)	1043
BseXI	GCAGC	4 cut(s)	283, 283, 865, 976
BseYI	CCCAGC	2 cut(s)	35, 173
BshFI	GGCC	3 cut(s)	664, 941, 1277
BshNI	GGYRCC	3 cut(s)	737, 998, 1038
BsiHKCI	CYCGRG	2 cut(s)	209, 919
BsiSI	CCGG	1 cut(s)	95
BslFI	GGGAC	3 cut(s)	170, 764, 848
BslI	CCNNNNNNNGG	2 cut(s)	926, 1108
BsmAI	GTCTC	4 cut(s)	80, 158, 916, 1314
BsmBI	CGTCTC	1 cut(s)	1314
BsmFI	GGGAC	3 cut(s)	170, 764, 848
BsmI	GAATGC	2 cut(s)	18, 574
BsnI	GGCC	3 cut(s)	664, 941, 1277
BsoBI	CYCGRG	2 cut(s)	209, 919
Bsp1286I	GDGCHC	1 cut(s)	1043
Bsp143I	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
BspACI	CCGC	3 cut(s)	640, 834, 1278
BspANI	GGCC	3 cut(s)	664, 941, 1277
BspCNI	CTCAG	5 cut(s)	538, 751, 842, 859, 1249
BspLI	GGNNCC	4 cut(s)	41, 739, 1000, 1040
BspOI	GCTAGC	1 cut(s)	900
BspPI	GGATC	5 cut(s)	34, 47, 354, 639, 1178
BspT107I	GGYRCC	3 cut(s)	737, 998, 1038
BsrI	ACTGG	9 cut(s)	172, 244, 394, 439, 821, 1040, 1051, 1223, 1287
BssECI	CCNNGG	3 cut(s)	188, 837, 958
BssMI	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
BssSI	CACGAG	1 cut(s)	848
BssT1I	CCWWGG	2 cut(s)	188, 837
Bst2BI	CACGAG	1 cut(s)	848
Bst2UI	CCWGG	2 cut(s)	44, 1092
Bst6I	CTCTTC	1 cut(s)	1196
BstAPI	GCANNNNNTGC	1 cut(s)	140
BstBAI	YACGTR	1 cut(s)	1265
BstC8I	GCNNGC	2 cut(s)	894, 898
BstDEI	CTNAG	7 cut(s)	495, 525, 759, 829, 867, 1107, 1257
BstEII	GGTNACC	1 cut(s)	1097
BstENI	CCTNNNNNAGG	1 cut(s)	1106
BstF5I	GGATG	3 cut(s)	262, 601, 707
BstKTI	GATC	9 cut(s)	42, 208, 349, 647, 702, 873, 1134, 1173, 1189
BstMAI	GTCTC	4 cut(s)	80, 158, 916, 1314
BstMBI	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
BstMWI	GCNNNNNNNGC	3 cut(s)	140, 839, 1205
BstNI	CCWGG	2 cut(s)	44, 1092
BstNSI	RCATGY	1 cut(s)	185
BstPI	GGTNACC	1 cut(s)	1097
BstSCI	CCNGG	2 cut(s)	42, 1090
BstSFI	CTRYAG	2 cut(s)	120, 474
BstSLI	GKGCMC	1 cut(s)	1043
BstV1I	GCAGC	4 cut(s)	283, 283, 865, 976
BstV2I	GAAGAC	2 cut(s)	190, 1275
BstX2I	RGATCY	3 cut(s)	39, 346, 644
BstXI	CCANNNNNNTGG	1 cut(s)	748
BstYI	RGATCY	3 cut(s)	39, 346, 644
Bsu36I	CCTNAGG	1 cut(s)	525
BsuI	GTATCC	1 cut(s)	803
BsuRI	GGCC	3 cut(s)	664, 941, 1277
BtsCI	GGATG	3 cut(s)	262, 601, 707
BtsIMutI	CAGTG	5 cut(s)	324, 565, 756, 1216, 1280
Cac8I	GCNNGC	2 cut(s)	894, 898
CaiI	CAGNNNCTG	1 cut(s)	866
Csp6I	GTAC	6 cut(s)	237, 243, 387, 934, 955, 1222
CviAII	CATG	6 cut(s)	83, 182, 802, 901, 937, 1192
CviQI	GTAC	6 cut(s)	237, 243, 387, 934, 955, 1222
DdeI	CTNAG	7 cut(s)	495, 525, 759, 829, 867, 1107, 1257
DpnI	GATC	9 cut(s)	41, 207, 348, 646, 701, 872, 1133, 1172, 1188
DpnII	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
EaeI	YGGCCR	1 cut(s)	1275
Eam1104I	CTCTTC	1 cut(s)	1196
EarI	CTCTTC	1 cut(s)	1196
Eco130I	CCWWGG	2 cut(s)	188, 837
Eco32I	GATATC	3 cut(s)	655, 693, 754
Eco57I	CTGAAG	1 cut(s)	1332
Eco72I	CACGTG	1 cut(s)	1265
Eco81I	CCTNAGG	1 cut(s)	525
Eco88I	CYCGRG	2 cut(s)	209, 919
Eco91I	GGTNACC	1 cut(s)	1097
EcoNI	CCTNNNNNAGG	1 cut(s)	1106
EcoO65I	GGTNACC	1 cut(s)	1097
EcoRI	GAATTC	1 cut(s)	1250
EcoRII	CCWGG	2 cut(s)	42, 1090
EcoRV	GATATC	3 cut(s)	655, 693, 754
EcoT14I	CCWWGG	2 cut(s)	188, 837
ErhI	CCWWGG	2 cut(s)	188, 837
Esp3I	CGTCTC	1 cut(s)	1314
FaeI	CATG	6 cut(s)	86, 185, 805, 904, 940, 1195
FaqI	GGGAC	3 cut(s)	170, 764, 848
FatI	CATG	6 cut(s)	82, 181, 801, 900, 936, 1191
FbaI	TGATCA	1 cut(s)	1131
Fnu4HI	GCNGC	7 cut(s)	272, 297, 640, 834, 854, 990, 1278
FokI	GGATG	3 cut(s)	249, 608, 694
Fsp4HI	GCNGC	7 cut(s)	272, 297, 640, 834, 854, 990, 1278
FspBI	CTAG	5 cut(s)	189, 312, 461, 650, 897
GluI	GCNGC	7 cut(s)	272, 297, 640, 834, 854, 990, 1278
GsaI	CCCAGC	2 cut(s)	39, 177
GsuI	CTGGAG	1 cut(s)	1304
HaeIII	GGCC	3 cut(s)	664, 941, 1277
HapII	CCGG	1 cut(s)	95
Hin1II	CATG	6 cut(s)	86, 185, 805, 904, 940, 1195
HincII	GTYRAC	2 cut(s)	254, 1033
HindII	GTYRAC	2 cut(s)	254, 1033
HindIII	AAGCTT	1 cut(s)	497
HinfI	GANTC	2 cut(s)	87, 1062
HpaII	CCGG	1 cut(s)	95
HphI	GGTGA	5 cut(s)	865, 1109, 1241, 1259, 1352
Hpy166II	GTNNAC	3 cut(s)	254, 1033, 1216
Hpy188I	TCNGA	4 cut(s)	29, 50, 1116, 1258
Hpy188III	TCNNGA	9 cut(s)	77, 209, 266, 356, 441, 584, 658, 766, 1006
Hpy8I	GTNNAC	3 cut(s)	254, 1033, 1216
Hpy99I	CGWCG	1 cut(s)	230
HpyAV	CCTTC	7 cut(s)	328, 528, 590, 1226, 1238, 1307, 1331
HpyCH4IV	ACGT	3 cut(s)	747, 1224, 1264
HpyCH4V	TGCA	3 cut(s)	134, 818, 1154
HpyF10VI	GCNNNNNNNGC	3 cut(s)	140, 839, 1205
HpyF3I	CTNAG	7 cut(s)	495, 525, 759, 829, 867, 1107, 1257
HpySE526I	ACGT	3 cut(s)	747, 1224, 1264
Hsp92II	CATG	6 cut(s)	86, 185, 805, 904, 940, 1195
Ksp22I	TGATCA	1 cut(s)	1131
Kzo9I	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
LmnI	GCTCC	1 cut(s)	64
Lsp1109I	GCAGC	4 cut(s)	283, 283, 865, 976
LweI	GCATC	3 cut(s)	96, 130, 283
MaeI	CTAG	5 cut(s)	189, 312, 461, 650, 897
MaeII	ACGT	3 cut(s)	747, 1224, 1264
MaeIII	GTNAC	3 cut(s)	914, 1097, 1136
MalI	GATC	9 cut(s)	41, 207, 348, 646, 701, 872, 1133, 1172, 1188
MboI	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
MboII	GAAGA	7 cut(s)	190, 302, 1213, 1280, 1310, 1340, 1343
MflI	RGATCY	3 cut(s)	39, 346, 644
MhlI	GDGCHC	1 cut(s)	1043
MluCI	AATT	3 cut(s)	52, 339, 1250
MmeI	TCCRAC	2 cut(s)	28, 1103
MroXI	GAANNNNTTC	1 cut(s)	436
MseI	TTAA	1 cut(s)	984
MslI	CAYNNNNRTG	2 cut(s)	594, 746
MspI	CCGG	1 cut(s)	95
MspR9I	CCNGG	2 cut(s)	44, 1092
Mva1269I	GAATGC	2 cut(s)	18, 574
MvaI	CCWGG	2 cut(s)	44, 1092
MwoI	GCNNNNNNNGC	3 cut(s)	140, 839, 1205
NdeII	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
NheI	GCTAGC	1 cut(s)	896
NlaIII	CATG	6 cut(s)	86, 185, 805, 904, 940, 1195
NlaIV	GGNNCC	4 cut(s)	41, 739, 1000, 1040
NmeAIII	GCCGAG	1 cut(s)	135
NmuCI	GTSAC	2 cut(s)	914, 1097
NspI	RCATGY	1 cut(s)	185
PaeR7I	CTCGAG	1 cut(s)	209
PcsI	WCGNNNNNNNCGW	2 cut(s)	207, 216
PctI	GAATGC	2 cut(s)	18, 574
PdmI	GAANNNNTTC	1 cut(s)	436
PfeI	GAWTC	2 cut(s)	87, 1062
PkrI	GCNGC	7 cut(s)	273, 298, 641, 835, 855, 991, 1279
PmaCI	CACGTG	1 cut(s)	1265
PmlI	CACGTG	1 cut(s)	1265
Ppu21I	YACGTR	1 cut(s)	1265
Psp6I	CCWGG	2 cut(s)	42, 1090
PspCI	CACGTG	1 cut(s)	1265
PspEI	GGTNACC	1 cut(s)	1097
PspFI	CCCAGC	2 cut(s)	35, 173
PspGI	CCWGG	2 cut(s)	42, 1090
PspN4I	GGNNCC	4 cut(s)	41, 739, 1000, 1040
PstNI	CAGNNNCTG	1 cut(s)	866
PsuI	RGATCY	3 cut(s)	39, 346, 644
RsaI	GTAC	6 cut(s)	238, 244, 388, 935, 956, 1223
RsaNI	GTAC	6 cut(s)	237, 243, 387, 934, 955, 1222
RseI	CAYNNNNRTG	2 cut(s)	594, 746
SaqAI	TTAA	1 cut(s)	984
SatI	GCNGC	7 cut(s)	272, 297, 640, 834, 854, 990, 1278
Sau3AI	GATC	9 cut(s)	39, 205, 346, 644, 699, 870, 1131, 1170, 1186
ScrFI	CCNGG	2 cut(s)	44, 1092
SduI	GDGCHC	1 cut(s)	1043
SfaNI	GCATC	3 cut(s)	96, 130, 283
SfcI	CTRYAG	2 cut(s)	120, 474
Sfr274I	CTCGAG	1 cut(s)	209
SlaI	CTCGAG	1 cut(s)	209
SmiMI	CAYNNNNRTG	2 cut(s)	594, 746
SmlI	CTYRAG	1 cut(s)	209
SmoI	CTYRAG	1 cut(s)	209
Sse9I	AATT	3 cut(s)	52, 339, 1250
SsiI	CCGC	3 cut(s)	640, 834, 1278
SspMI	CTAG	5 cut(s)	189, 312, 461, 650, 897
StyD4I	CCNGG	2 cut(s)	42, 1090
StyI	CCWWGG	2 cut(s)	188, 837
TaiI	ACGT	3 cut(s)	750, 1227, 1267
TaqI	TCGA	5 cut(s)	78, 210, 225, 657, 1173
TasI	AATT	3 cut(s)	52, 339, 1250
TatI	WGTACW	2 cut(s)	386, 933
TauI	GCSGC	3 cut(s)	642, 836, 1280
TfiI	GAWTC	2 cut(s)	87, 1062
Tru1I	TTAA	1 cut(s)	984
Tru9I	TTAA	1 cut(s)	984
TscAI	CASTG	5 cut(s)	331, 565, 763, 1223, 1287
TseFI	GTSAC	2 cut(s)	914, 1097
TseI	GCWGC	4 cut(s)	271, 296, 853, 989
Tsp45I	GTSAC	2 cut(s)	914, 1097
TspDTI	ATGAA	4 cut(s)	696, 790, 1341, 1350
TspGWI	ACGGA	4 cut(s)	114, 608, 942, 1183
TspRI	CASTG	5 cut(s)	331, 565, 763, 1223, 1287
XagI	CCTNNNNNAGG	1 cut(s)	1106
XapI	RAATTY	2 cut(s)	52, 1250
XceI	RCATGY	1 cut(s)	185
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1223
XhoI	CTCGAG	1 cut(s)	209
XmaJI	CCTAGG	1 cut(s)	188
XmnI	GAANNNNTTC	1 cut(s)	436
XspI	CTAG	5 cut(s)	189, 312, 461, 650, 897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.