MD03G1004400.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014912
Physical Location & Seq
Forward (+)
344948 .. 348190
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1004400.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGAGAGAAATCATAAGCATTCATATTGGGCAGGCTGGAATTCAGGTGGGCAATTCATGCTGGGAGCTCTACTGCCTCGAGCATGGAATTCAACCTGATGGCCAGATGCCCAGTGATACCACAGTGGGCGGTGGCCACGATGCTTTCAACACCTTCTTCAGCGAGACTGGATCAGGCAAGCATGTCCCAAGAGCCATATTTGTTGATCTCGAACCAACTGTTATTGATGAAGTTAGGACTGGGTCTTACCGCCAACTCTTCCACCCTGAGCAGCTCATTTCTGGAAAGGAAGATGCTGCTAATAATTTCGCAAGAGGACACTATACAGTTGGAAAGGAAATTGTAGATCTCTGTCTTGATCGAGTTAGGAAATTGGCCGATAATTGCACTGGTTTACAAGGATTTCTGGTGTTCAATGCTGTTGGTGGTGGTACTGGTTCTGGTTTGGGGTCCTTGCTCTTAGAACGCTTGTCGGTGGATTATGGAAAGAAGTCAAAGCTTGGGTTCACCATCTATCCTTCTCCGCAGGTTTCAACAGCAGTTGTTGAGCCTTATAATAGTGTTCTCTCTACTCATGCCCTCCTTGAACACACAGATGTATCTGTGCTCTTGGACAATGAAGCTATCTATGATATTTGCAGGAGATCCCTAGACATCGAGAGACCAACTTACACCAATTTGAATAGACTGATATCACAAGTCATATCCTCCTTGACAACATCATTGAGATTTGATGGAGCCATTAATGTTGATGTGACAGAGTTCCAGACAAACCTTGTTCCATATCCCCGCATCCATTTCATGCTTTCCTCATATGCCCCTGTTATCTCAGCTGCTAAGGCATACCATGAGCAGATCTCAATCCCTGAGATCACAAATGCAGTTTTTGAACCCTCAAGCATGATGGCCAAGTGTGATCCCCGGCATGGAAAATACATGGCCTGCTGCCTAATGTACAGGGGTGATGTAGTTCCCAAGGATGTCAATGCTGCTGTTGCAACTATCAAAACAAAAAGGACTGTGCAGTTTGTTGACTGGTGCCCAACAGGTTTCAAATGTGGTATTAACTATCAGCCTCCAACAGTTGTACCAGGGGGTGATCTTGCCAAGGTTCAGCGAGCAGTGTGCATGATCAGCAACAACACGGCAGTGGCTGAGGTGTTCTCACGCATTGACCACAAATTTGACCTCATGTATTCCAAGAGGGCATTTGTTCACTGGTATGTCGGTGAGGGAATGGAAGAAGGAGAGTTTTCAGAAGCTCGTGAAGATCTGGCTGCTCTAGAGAAAGATTACGAGGAAGTTGGTGCCGAAGGTGTTGATGATGAAGCGGGAAGTGAAGATTACTAG

Protein Analysis

450

Amino Acids

49.47

Weight (kDa)

5.07

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 7.5e-63 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 263 - 392 2.2e-51 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19770 AT5G19780
fragaria_vesca FvH4_3g45490
malus_domestica MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis pycom03g00450 pycom11g00410
rosa_chinensis RchiOBHm_Chr5g0083281
rosa_laevigata RLG00000037026
rosa_multiflora Rmu_sc0011792.1_g000008
rosa_roxburghii Rroxscaffold_1G00001210
rosa_rugosa Rorug05G0484000
rosa_samantha Rh5BG560700
rosa_wichuraiana Rw5G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 2 cut(s) 1038, 1307
AciI CCGC 5 cut(s) 129, 250, 524, 790, 1331
AclWI GGATC 3 cut(s) 178, 639, 911
AcoI YGGCCR 4 cut(s) 100, 133, 375, 906
AcsI RAATTY 3 cut(s) 39, 87, 1181
AcuI CTGAAG 1 cut(s) 142
AfaI GTAC 3 cut(s) 433, 956, 1089
AfiI CCNNNNNNNGG 1 cut(s) 926
AgsI TTSAA 8 cut(s) 92, 148, 415, 534, 587, 682, 890, 1054
AjnI CCWGG 1 cut(s) 1090
AleI CACNNNNGTG 1 cut(s) 1148
AluBI AGCT 6 cut(s) 67, 274, 499, 623, 833, 1262
AluI AGCT 6 cut(s) 67, 274, 499, 623, 833, 1262
Alw21I GWGCWC 2 cut(s) 69, 609
Alw26I GTCTC 2 cut(s) 158, 655
AlwI GGATC 3 cut(s) 178, 639, 911
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 5 cut(s) 100, 133, 375, 906, 939
ApeKI GCWGC 6 cut(s) 271, 296, 833, 945, 989, 1277
ApoI RAATTY 3 cut(s) 39, 87, 1181
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AseI ATTAAT 1 cut(s) 744
AspS9I GGNCC 1 cut(s) 450
AsuC2I CCSGG 1 cut(s) 922
AsuHPI GGTGA 4 cut(s) 499, 974, 1109, 1241
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 1 cut(s) 450
BaeGI GKGCMC 1 cut(s) 1043
BaeI ACNNNNGTAYC 6 cut(s) 108, 141, 582, 615, 1071, 1104
BalI TGGCCA 3 cut(s) 102, 135, 908
BanI GGYRCC 2 cut(s) 1038, 1307
BanII GRGCYC 1 cut(s) 69
BauI CACGAG 1 cut(s) 1263
Bbv12I GWGCWC 2 cut(s) 69, 609
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 6 cut(s) 283, 283, 820, 932, 976, 1264
BccI CCATC 4 cut(s) 92, 518, 728, 898
BceAI ACGGC 1 cut(s) 1161
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 1 cut(s) 1092
BclI TGATCA 1 cut(s) 1131
BcnI CCSGG 1 cut(s) 922
BcoDI GTCTC 2 cut(s) 158, 655
BfaI CTAG 3 cut(s) 650, 1283, 1348
BfuAI ACCTGC 1 cut(s) 517
BglII AGATCT 3 cut(s) 346, 855, 1270
BisI GCNGC 6 cut(s) 272, 297, 834, 946, 990, 1278
BlsI GCNGC 6 cut(s) 273, 298, 835, 947, 991, 1279
Bme1390I CCNGG 2 cut(s) 922, 1092
Bme18I GGWCC 1 cut(s) 450
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 4 cut(s) 451, 739, 1040, 1309
BmrFI CCNGG 2 cut(s) 922, 1092
BmrI ACTGGG 2 cut(s) 105, 249
BmsI GCATC 4 cut(s) 96, 130, 283, 801
BmuI ACTGGG 2 cut(s) 105, 249
BplI GAGNNNNNCTC 4 cut(s) 842, 874, 1148, 1180
Bpu10I CCTNAGC 3 cut(s) 267, 837, 1155
BpuEI CTTGAG 1 cut(s) 880
BpuMI CCSGG 1 cut(s) 922
BsaBI GATNNNNATC 1 cut(s) 860
BsaI GGTCTC 1 cut(s) 655
BsaJI CCNNGG 4 cut(s) 920, 975, 1091, 1107
Bsc4I CCNNNNNNNGG 1 cut(s) 926
Bse1I ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1223
Bse8I GATNNNNATC 1 cut(s) 860
BseBI CCWGG 1 cut(s) 1092
BseDI CCNNGG 4 cut(s) 920, 975, 1091, 1107
BseGI GGATG 2 cut(s) 792, 985
BseJI GATNNNNATC 1 cut(s) 860
BseLI CCNNNNNNNGG 1 cut(s) 926
BseMII CTCAG 4 cut(s) 258, 843, 858, 1146
BseNI ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1223
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 6 cut(s) 283, 283, 820, 932, 976, 1264
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 1 cut(s) 1043
BshFI GGCC 5 cut(s) 102, 135, 377, 908, 941
BshNI GGYRCC 2 cut(s) 1038, 1307
BsiHKAI GWGCWC 2 cut(s) 69, 609
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 1 cut(s) 922
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 926
BsmAI GTCTC 2 cut(s) 158, 655
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 5 cut(s) 102, 135, 377, 908, 941
Bso31I GGTCTC 1 cut(s) 655
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 3 cut(s) 69, 609, 1043
Bsp1407I TGTACA 1 cut(s) 954
BspACI CCGC 5 cut(s) 129, 250, 524, 790, 1331
BspANI GGCC 5 cut(s) 102, 135, 377, 908, 941
BspCNI CTCAG 4 cut(s) 259, 842, 859, 1147
BspLI GGNNCC 4 cut(s) 451, 739, 1040, 1309
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 3 cut(s) 178, 639, 911
BspT107I GGYRCC 2 cut(s) 1038, 1307
BspTNI GGTCTC 1 cut(s) 655
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1223
BssECI CCNNGG 4 cut(s) 920, 975, 1091, 1107
BssSI CACGAG 1 cut(s) 1263
BssT1I CCWWGG 2 cut(s) 975, 1107
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 1 cut(s) 1092
Bst4CI ACNGT 5 cut(s) 124, 220, 328, 1021, 1084
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 954
BstC8I GCNNGC 4 cut(s) 33, 179, 943, 1119
BstDEI CTNAG 6 cut(s) 267, 460, 829, 837, 867, 1155
BstF5I GGATG 2 cut(s) 792, 985
BstMAI GTCTC 2 cut(s) 158, 655
BstMWI GCNNNNNNNGC 4 cut(s) 57, 839, 995, 1134
BstNI CCWGG 1 cut(s) 1092
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 920, 1090
BstSLI GKGCMC 1 cut(s) 1043
BstV1I GCAGC 6 cut(s) 283, 283, 820, 932, 976, 1264
BstX2I RGATCY 4 cut(s) 346, 644, 855, 1270
BstYI RGATCY 4 cut(s) 346, 644, 855, 1270
BsuRI GGCC 5 cut(s) 102, 135, 377, 908, 941
BtsCI GGATG 2 cut(s) 792, 985
BtsI GCAGTG 2 cut(s) 1128, 1155
BtsIMutI CAGTG 6 cut(s) 118, 129, 387, 1128, 1155, 1216
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 4 cut(s) 33, 179, 943, 1119
CaiI CAGNNNCTG 1 cut(s) 1154
Cfr13I GGNCC 1 cut(s) 450
Csp6I GTAC 3 cut(s) 432, 955, 1088
CviQI GTAC 3 cut(s) 432, 955, 1088
DdeI CTNAG 6 cut(s) 267, 460, 829, 837, 867, 1155
EaeI YGGCCR 4 cut(s) 100, 133, 375, 906
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 975, 1107
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 655
Eco32I GATATC 1 cut(s) 693
Eco47I GGWCC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 450
EcoRI GAATTC 2 cut(s) 39, 87
EcoRII CCWGG 1 cut(s) 1090
EcoRV GATATC 1 cut(s) 693
EcoT14I CCWWGG 2 cut(s) 975, 1107
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 2 cut(s) 975, 1107
FaqI GGGAC 1 cut(s) 170
FauI CCCGC 2 cut(s) 797, 1324
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 1 cut(s) 1131
Fnu4HI GCNGC 6 cut(s) 272, 297, 834, 946, 990, 1278
FokI GGATG 2 cut(s) 779, 992
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 6 cut(s) 272, 297, 834, 946, 990, 1278
FspBI CTAG 3 cut(s) 650, 1283, 1348
GluI GCNGC 6 cut(s) 272, 297, 834, 946, 990, 1278
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 5 cut(s) 102, 135, 377, 908, 941
HapII CCGG 1 cut(s) 922
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 497
HpaII CCGG 1 cut(s) 922
HphI GGTGA 4 cut(s) 499, 974, 1109, 1241
Hpy166II GTNNAC 4 cut(s) 395, 507, 1033, 1216
Hpy188I TCNGA 1 cut(s) 1258
Hpy188III TCNNGA 7 cut(s) 209, 282, 356, 658, 766, 1265, 1283
Hpy8I GTNNAC 4 cut(s) 395, 507, 1033, 1216
HpyAV CCTTC 4 cut(s) 163, 528, 1238, 1307
HpyCH4III ACNGT 5 cut(s) 124, 220, 328, 1021, 1084
HpyCH4V TGCA 6 cut(s) 387, 639, 881, 998, 1024, 1128
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 839, 995, 1134
HpyF3I CTNAG 6 cut(s) 267, 460, 829, 837, 867, 1155
Ksp22I TGATCA 1 cut(s) 1131
LmnI GCTCC 2 cut(s) 64, 737
Lsp1109I GCAGC 6 cut(s) 283, 283, 820, 932, 976, 1264
LweI GCATC 4 cut(s) 96, 130, 283, 801
MaeI CTAG 3 cut(s) 650, 1283, 1348
MaeIII GTNAC 1 cut(s) 754
MboII GAAGA 5 cut(s) 148, 250, 302, 1253, 1280
MflI RGATCY 4 cut(s) 346, 644, 855, 1270
MhlI GDGCHC 3 cut(s) 69, 609, 1043
MlsI TGGCCA 3 cut(s) 102, 135, 908
MluCI AATT 9 cut(s) 39, 52, 87, 304, 339, 371, 382, 676, 1181
MluNI TGGCCA 3 cut(s) 102, 135, 908
MmeI TCCRAC 2 cut(s) 310, 1103
Mox20I TGGCCA 3 cut(s) 102, 135, 908
MscI TGGCCA 3 cut(s) 102, 135, 908
MseI TTAA 2 cut(s) 744, 1065
MslI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
Msp20I TGGCCA 3 cut(s) 102, 135, 908
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 1 cut(s) 922
MspR9I CCNGG 2 cut(s) 922, 1092
Mva1269I GAATGC 1 cut(s) 18
MvaI CCWGG 1 cut(s) 1092
MwoI GCNNNNNNNGC 4 cut(s) 57, 839, 995, 1134
NciI CCSGG 1 cut(s) 922
NdeI CATATG 1 cut(s) 814
NlaIV GGNNCC 4 cut(s) 451, 739, 1040, 1309
NmuCI GTSAC 1 cut(s) 754
NspI RCATGY 1 cut(s) 185
OliI CACNNNNGTG 1 cut(s) 1148
PaeR7I CTCGAG 1 cut(s) 77
PctI GAATGC 1 cut(s) 18
PflFI GACNNNGTC 1 cut(s) 241
PkrI GCNGC 6 cut(s) 273, 298, 835, 947, 991, 1279
PpuMI RGGWCCY 1 cut(s) 450
PshBI ATTAAT 1 cut(s) 744
PsiI TTATAA 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 1090
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 1090
PspN4I GGNNCC 4 cut(s) 451, 739, 1040, 1309
PspPI GGNCC 1 cut(s) 450
PspPPI RGGWCCY 1 cut(s) 450
PspXI VCTCGAGB 1 cut(s) 77
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 4 cut(s) 346, 644, 855, 1270
PsyI GACNNNGTC 1 cut(s) 241
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 3 cut(s) 433, 956, 1089
RsaNI GTAC 3 cut(s) 432, 955, 1088
RseI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 744, 1065
SatI GCNGC 6 cut(s) 272, 297, 834, 946, 990, 1278
Sau96I GGNCC 1 cut(s) 450
ScrFI CCNGG 2 cut(s) 922, 1092
SduI GDGCHC 3 cut(s) 69, 609, 1043
SfaNI GCATC 4 cut(s) 96, 130, 283, 801
Sfr274I CTCGAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 450
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SmlI CTYRAG 2 cut(s) 77, 895
SmoI CTYRAG 2 cut(s) 77, 895
Sse9I AATT 9 cut(s) 39, 52, 87, 304, 339, 371, 382, 676, 1181
SsiI CCGC 5 cut(s) 129, 250, 524, 790, 1331
SspMI CTAG 3 cut(s) 650, 1283, 1348
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 920, 1090
StyI CCWWGG 2 cut(s) 975, 1107
TaaI ACNGT 5 cut(s) 124, 220, 328, 1021, 1084
TaqI TCGA 4 cut(s) 78, 210, 361, 657
TasI AATT 9 cut(s) 39, 52, 87, 304, 339, 371, 382, 676, 1181
TatI WGTACW 1 cut(s) 954
Tru1I TTAA 2 cut(s) 744, 1065
Tru9I TTAA 2 cut(s) 744, 1065
TscAI CASTG 6 cut(s) 118, 129, 394, 1128, 1155, 1223
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 6 cut(s) 271, 296, 833, 945, 989, 1277
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 6 cut(s) 11, 45, 243, 633, 790, 1341
TspRI CASTG 6 cut(s) 118, 129, 394, 1128, 1155, 1223
Tth111I GACNNNGTC 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 450
VspI ATTAAT 1 cut(s) 744
XapI RAATTY 3 cut(s) 39, 87, 1181
XbaI TCTAGA 1 cut(s) 1282
XceI RCATGY 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 77
XspI CTAG 3 cut(s) 650, 1283, 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.