Prupe.6G004100_v2.0.a1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0014912
Physical Location & Seq
Forward (+)
421362 .. 424286
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G004100.1

Sequence Viewer

Length: 1353 bp
ATGAGGGAGATTATAAGCATACATATTGGGCAGGCTGGAATTCAGGTGGGGAATTCATGTTGGGAACTCTATTGTCTTGAGCATGGAATTCAACCCGATGGGCAGATGCCCAGTGATGCCTCAGTGGGTGTTGGCCGCGACGCTTTCAATACCTTCTTCAGTGAGACTGGATCAGGCAAGCACGTCCCTAGGGCCATATTTGTCGATTTGGAACCAACTGTTATTGATGAAGTAAGGACTGGGACTTACCGCCAACTCTTCCACCCCGAGCAGCTCATTTCTGGGAAAGAAGATGCTGCTAATAATTTTGCAAGAGGACACTATACAGTCGGAAAGGAAATTGTAGATCTCTGCCTTGATCGAGTAAGGAAATTGGCCGATAATTGCACTGGTTTACAAGGATTTTTGGTGTTCAATGCTGTTGGTGGTGGTACTGGTTCTGGTTTGGGGTCCTTGCTGTTAGAACGGTTGTCTGTAGATTATGGAAAGAAGTCAAAGCTTGGATTCACCATCTATCCTTCTCCGCAGGTTTCAACAGCAGTTGTCGAGCCTTACAATAGTGTTCTCTCTACTCATTCCCTCCTTGAACACACAGATGTATCTGTGCTCTTGGACAATGAAGCTATCTATGACATTTGCAGGAGATCTCTAGACATCGAGAGACCAACATACACCAATCTGAACAGATTGATATCGCAAGTCATATCATCTTTGACAACATCATTGAGATTTGATGGAGCCATTAATGTTGATGTCACAGAGTTCCAGACTAACCTTGTACCATATCCCCGCATTCATTTCATGCTTTCCTCATATGCTCCTGTTATCTCAGCTGAAAAGGCATATCACGAGCAGATCTCTATCCCTGAGATCACAAATGCAGTTTTTGAGCCCTCAAGCATGATGGCCAAGTGTGATCCCAGGCATGGAAAATACATGGCCTGCTGCTTAATGTACAGGGGAGATGTTGTCCCCAAGGATGTTAATGCCGCTGTTGCTACTATCAAAACAAAAAGGACTGTACAGTTTGTTGACTGGTGCCCAACAGGCTTCAAATGTGGCATTAACTATCAGCCTCCGACAGTTGTACCGGGGGGTGATCTTGCCAAGGTGCAGCGAGCAGTGTGCATGATCAGCAACAACACAGCAGTGGCTGAGGTGTTCTCACGCATTGACCACAAATTTGATCTCATGTATTCGAAGAGGGCATTCGTTCACTGGTATGTTGGTGAAGGCATGGAAGAAGGAGAGTTTTCTGAAGCTCGTGAAGATCTGGCTGCTCTAGAGAAAGATTACGAGGAGGTTGGTGCTGAAGGTGTAGATGATGAAGAGGGGGGAAGTGAAGATTACTGA

Protein Analysis

451

Amino Acids

49.69

Weight (kDa)

5.0

Isoelectric Point (pI)

35.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19770 AT5G19780
fragaria_vesca FvH4_3g45490
malus_domestica MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis pycom03g00450 pycom11g00410
rosa_chinensis RchiOBHm_Chr5g0083281
rosa_laevigata RLG00000037026
rosa_multiflora Rmu_sc0011792.1_g000008
rosa_roxburghii Rroxscaffold_1G00001210
rosa_rugosa Rorug05G0484000
rosa_samantha Rh5BG560700
rosa_wichuraiana Rw5G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 1038
AccII CGCG 1 cut(s) 138
AciI CCGC 5 cut(s) 136, 250, 524, 790, 990
AclWI GGATC 2 cut(s) 178, 911
AcoI YGGCCR 3 cut(s) 133, 375, 906
AcsI RAATTY 4 cut(s) 39, 52, 87, 1181
AcuI CTGAAG 3 cut(s) 142, 1278, 1332
AfaI GTAC 5 cut(s) 433, 780, 956, 1023, 1089
AfiI CCNNNNNNNGG 1 cut(s) 926
AgsI TTSAA 6 cut(s) 92, 148, 415, 534, 587, 1054
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 1 cut(s) 920
AleI CACNNNNGTG 1 cut(s) 1148
AluBI AGCT 5 cut(s) 274, 499, 623, 833, 1262
AluI AGCT 5 cut(s) 274, 499, 623, 833, 1262
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 2 cut(s) 158, 655
AlwI GGATC 2 cut(s) 178, 911
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 5 cut(s) 133, 192, 375, 906, 939
ApeKI GCWGC 5 cut(s) 271, 296, 945, 1114, 1277
ApoI RAATTY 4 cut(s) 39, 52, 87, 1181
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AseI ATTAAT 1 cut(s) 744
AspA2I CCTAGG 1 cut(s) 188
AspS9I GGNCC 2 cut(s) 192, 450
AsuC2I CCSGG 1 cut(s) 1092
AsuHPI GGTGA 3 cut(s) 499, 1109, 1241
AsuII TTCGAA 1 cut(s) 1199
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 1043
BaeI ACNNNNGTAYC 4 cut(s) 582, 615, 1071, 1104
BalI TGGCCA 1 cut(s) 908
BanI GGYRCC 1 cut(s) 1038
BanII GRGCYC 1 cut(s) 894
BauI CACGAG 2 cut(s) 848, 1263
Bbv12I GWGCWC 1 cut(s) 609
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 5 cut(s) 283, 283, 932, 1126, 1264
BccI CCATC 4 cut(s) 92, 518, 728, 898
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 1 cut(s) 922
BclI TGATCA 1 cut(s) 1131
BcnI CCSGG 1 cut(s) 1092
BcoDI GTCTC 2 cut(s) 158, 655
BfaI CTAG 3 cut(s) 189, 650, 1283
BfmI CTRYAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 517
BglI GCCNNNNNGGC 1 cut(s) 1047
BglII AGATCT 4 cut(s) 346, 644, 855, 1270
BisI GCNGC 7 cut(s) 136, 272, 297, 946, 990, 1115, 1278
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 7 cut(s) 137, 273, 298, 947, 991, 1116, 1279
Bme1390I CCNGG 2 cut(s) 922, 1092
Bme18I GGWCC 1 cut(s) 450
BmeT110I CYCGRG 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 192, 450
BmiI GGNNCC 4 cut(s) 213, 451, 739, 1040
BmrFI CCNGG 2 cut(s) 922, 1092
BmrI ACTGGG 2 cut(s) 105, 249
BmsI GCATC 3 cut(s) 96, 106, 283
BmuI ACTGGG 2 cut(s) 105, 249
BplI GAGNNNNNCTC 4 cut(s) 842, 874, 1148, 1180
Bpu10I CCTNAGC 1 cut(s) 1155
Bpu14I TTCGAA 1 cut(s) 1199
BpuEI CTTGAG 2 cut(s) 98, 880
BpuMI CCSGG 1 cut(s) 1092
BsaBI GATNNNNATC 2 cut(s) 691, 860
BsaI GGTCTC 1 cut(s) 655
BsaJI CCNNGG 5 cut(s) 188, 920, 975, 1091, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 1 cut(s) 926
Bse1I ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1223
Bse8I GATNNNNATC 2 cut(s) 691, 860
BseBI CCWGG 1 cut(s) 922
BseDI CCNNGG 5 cut(s) 188, 920, 975, 1091, 1107
BseGI GGATG 1 cut(s) 985
BseJI GATNNNNATC 2 cut(s) 691, 860
BseLI CCNNNNNNNGG 1 cut(s) 926
BseMII CTCAG 4 cut(s) 135, 843, 858, 1146
BseNI ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1223
BseRI GAGGAG 1 cut(s) 1313
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 5 cut(s) 283, 283, 932, 1126, 1264
BsgI GTGCAG 1 cut(s) 1133
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 5 cut(s) 135, 194, 377, 908, 941
BshNI GGYRCC 1 cut(s) 1038
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 1091
BslFI GGGAC 3 cut(s) 170, 256, 956
BslI CCNNNNNNNGG 1 cut(s) 926
BsmAI GTCTC 2 cut(s) 158, 655
BsmFI GGGAC 3 cut(s) 170, 256, 956
BsmI GAATGC 2 cut(s) 792, 1208
BsnI GGCC 5 cut(s) 135, 194, 377, 908, 941
Bso31I GGTCTC 1 cut(s) 655
BsoBI CYCGRG 1 cut(s) 266
Bsp119I TTCGAA 1 cut(s) 1199
Bsp1286I GDGCHC 3 cut(s) 609, 894, 1043
Bsp1407I TGTACA 2 cut(s) 954, 1021
BspACI CCGC 5 cut(s) 136, 250, 524, 790, 990
BspANI GGCC 5 cut(s) 135, 194, 377, 908, 941
BspCNI CTCAG 4 cut(s) 134, 842, 859, 1147
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 213, 451, 739, 1040
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 2 cut(s) 178, 911
BspT104I TTCGAA 1 cut(s) 1199
BspT107I GGYRCC 1 cut(s) 1038
BspTNI GGTCTC 1 cut(s) 655
BsrGI TGTACA 2 cut(s) 954, 1021
BsrI ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1223
BssECI CCNNGG 5 cut(s) 188, 920, 975, 1091, 1107
BssSI CACGAG 2 cut(s) 848, 1263
BssT1I CCWWGG 3 cut(s) 188, 975, 1107
Bst2BI CACGAG 2 cut(s) 848, 1263
Bst2UI CCWGG 1 cut(s) 922
Bst4CI ACNGT 6 cut(s) 220, 328, 468, 1021, 1026, 1084
Bst6I CTCTTC 3 cut(s) 263, 1196, 1323
BstAUI TGTACA 2 cut(s) 954, 1021
BstBI TTCGAA 1 cut(s) 1199
BstC8I GCNNGC 4 cut(s) 33, 179, 943, 1119
BstDEI CTNAG 4 cut(s) 121, 829, 867, 1155
BstF5I GGATG 1 cut(s) 985
BstFNI CGCG 1 cut(s) 138
BstMAI GTCTC 2 cut(s) 158, 655
BstMWI GCNNNNNNNGC 4 cut(s) 839, 995, 1047, 1134
BstNI CCWGG 1 cut(s) 922
BstSCI CCNGG 2 cut(s) 920, 1090
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 1043
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 5 cut(s) 283, 283, 932, 1126, 1264
BstX2I RGATCY 4 cut(s) 346, 644, 855, 1270
BstYI RGATCY 4 cut(s) 346, 644, 855, 1270
BsuRI GGCC 5 cut(s) 135, 194, 377, 908, 941
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 1 cut(s) 985
BtsI GCAGTG 2 cut(s) 1128, 1155
BtsIMutI CAGTG 7 cut(s) 118, 129, 166, 387, 1128, 1155, 1216
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 4 cut(s) 33, 179, 943, 1119
CaiI CAGNNNCTG 1 cut(s) 1154
Cfr13I GGNCC 2 cut(s) 192, 450
CseI GACGC 1 cut(s) 149
Csp6I GTAC 5 cut(s) 432, 779, 955, 1022, 1088
CviAII CATG 9 cut(s) 57, 83, 802, 901, 926, 937, 1129, 1192, 1237
CviQI GTAC 5 cut(s) 432, 779, 955, 1022, 1088
DdeI CTNAG 4 cut(s) 121, 829, 867, 1155
EaeI YGGCCR 3 cut(s) 133, 375, 906
Eam1104I CTCTTC 3 cut(s) 263, 1196, 1323
EarI CTCTTC 3 cut(s) 263, 1196, 1323
Eco130I CCWWGG 3 cut(s) 188, 975, 1107
Eco24I GRGCYC 1 cut(s) 894
Eco31I GGTCTC 1 cut(s) 655
Eco32I GATATC 1 cut(s) 693
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 3 cut(s) 142, 1278, 1332
Eco88I CYCGRG 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 450
EcoRI GAATTC 3 cut(s) 39, 52, 87
EcoRII CCWGG 1 cut(s) 920
EcoRV GATATC 1 cut(s) 693
EcoT14I CCWWGG 3 cut(s) 188, 975, 1107
EcoT38I GRGCYC 1 cut(s) 894
ErhI CCWWGG 3 cut(s) 188, 975, 1107
FaeI CATG 9 cut(s) 60, 86, 805, 904, 929, 940, 1132, 1195, 1240
FaqI GGGAC 3 cut(s) 170, 256, 956
FatI CATG 9 cut(s) 56, 82, 801, 900, 925, 936, 1128, 1191, 1236
FauI CCCGC 1 cut(s) 797
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 1 cut(s) 1131
Fnu4HI GCNGC 7 cut(s) 136, 272, 297, 946, 990, 1115, 1278
FokI GGATG 1 cut(s) 992
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 7 cut(s) 136, 272, 297, 946, 990, 1115, 1278
FspBI CTAG 3 cut(s) 189, 650, 1283
GluI GCNGC 7 cut(s) 136, 272, 297, 946, 990, 1115, 1278
HaeIII GGCC 5 cut(s) 135, 194, 377, 908, 941
HapII CCGG 1 cut(s) 1091
HgaI GACGC 1 cut(s) 149
Hin1II CATG 9 cut(s) 60, 86, 805, 904, 929, 940, 1132, 1195, 1240
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 504
HpaII CCGG 1 cut(s) 1091
HphI GGTGA 3 cut(s) 499, 1109, 1241
Hpy166II GTNNAC 3 cut(s) 395, 1033, 1216
Hpy188I TCNGA 4 cut(s) 332, 681, 1080, 1258
Hpy188III TCNNGA 7 cut(s) 77, 650, 658, 766, 848, 1265, 1283
Hpy8I GTNNAC 3 cut(s) 395, 1033, 1216
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 5 cut(s) 163, 528, 1226, 1238, 1307
HpyCH4III ACNGT 6 cut(s) 220, 328, 468, 1021, 1026, 1084
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 6 cut(s) 311, 387, 639, 881, 1114, 1128
HpyF10VI GCNNNNNNNGC 4 cut(s) 839, 995, 1047, 1134
HpyF3I CTNAG 4 cut(s) 121, 829, 867, 1155
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 9 cut(s) 60, 86, 805, 904, 929, 940, 1132, 1195, 1240
Ksp22I TGATCA 1 cut(s) 1131
LmnI GCTCC 2 cut(s) 737, 823
Lsp1109I GCAGC 5 cut(s) 283, 283, 932, 1126, 1264
LweI GCATC 3 cut(s) 96, 106, 283
MaeI CTAG 3 cut(s) 189, 650, 1283
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 754
MboII GAAGA 7 cut(s) 148, 250, 302, 1213, 1253, 1280, 1340
MflI RGATCY 4 cut(s) 346, 644, 855, 1270
MhlI GDGCHC 3 cut(s) 609, 894, 1043
MlsI TGGCCA 1 cut(s) 908
MluCI AATT 8 cut(s) 39, 52, 87, 304, 339, 371, 382, 1181
MluNI TGGCCA 1 cut(s) 908
MmeI TCCRAC 2 cut(s) 310, 1103
Mox20I TGGCCA 1 cut(s) 908
MscI TGGCCA 1 cut(s) 908
MseI TTAA 4 cut(s) 744, 950, 984, 1065
MslI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
Msp20I TGGCCA 1 cut(s) 908
MspA1I CMGCKG 2 cut(s) 833, 992
MspI CCGG 1 cut(s) 1091
MspR9I CCNGG 2 cut(s) 922, 1092
Mva1269I GAATGC 2 cut(s) 792, 1208
MvaI CCWGG 1 cut(s) 922
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 839, 995, 1047, 1134
NciI CCSGG 1 cut(s) 1092
NdeI CATATG 1 cut(s) 814
NlaIII CATG 9 cut(s) 60, 86, 805, 904, 929, 940, 1132, 1195, 1240
NlaIV GGNNCC 4 cut(s) 213, 451, 739, 1040
NmuCI GTSAC 1 cut(s) 754
NspV TTCGAA 1 cut(s) 1199
OliI CACNNNNGTG 1 cut(s) 1148
PctI GAATGC 2 cut(s) 792, 1208
PfeI GAWTC 1 cut(s) 504
PkrI GCNGC 7 cut(s) 137, 273, 298, 947, 991, 1116, 1279
PpuMI RGGWCCY 1 cut(s) 450
PshBI ATTAAT 1 cut(s) 744
PsiI TTATAA 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 920
PspGI CCWGG 1 cut(s) 920
PspN4I GGNNCC 4 cut(s) 213, 451, 739, 1040
PspPI GGNCC 2 cut(s) 192, 450
PspPPI RGGWCCY 1 cut(s) 450
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 4 cut(s) 346, 644, 855, 1270
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 5 cut(s) 433, 780, 956, 1023, 1089
RsaNI GTAC 5 cut(s) 432, 779, 955, 1022, 1088
RseI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SaqAI TTAA 4 cut(s) 744, 950, 984, 1065
SatI GCNGC 7 cut(s) 136, 272, 297, 946, 990, 1115, 1278
Sau96I GGNCC 2 cut(s) 192, 450
ScrFI CCNGG 2 cut(s) 922, 1092
SduI GDGCHC 3 cut(s) 609, 894, 1043
SfaNI GCATC 3 cut(s) 96, 106, 283
SfcI CTRYAG 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 1199
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SmlI CTYRAG 2 cut(s) 77, 895
SmoI CTYRAG 2 cut(s) 77, 895
Sse9I AATT 8 cut(s) 39, 52, 87, 304, 339, 371, 382, 1181
SsiI CCGC 5 cut(s) 136, 250, 524, 790, 990
SspMI CTAG 3 cut(s) 189, 650, 1283
StyD4I CCNGG 2 cut(s) 920, 1090
StyI CCWWGG 3 cut(s) 188, 975, 1107
TaaI ACNGT 6 cut(s) 220, 328, 468, 1021, 1026, 1084
TaiI ACGT 1 cut(s) 186
TaqI TCGA 5 cut(s) 204, 361, 546, 657, 1199
TasI AATT 8 cut(s) 39, 52, 87, 304, 339, 371, 382, 1181
TatI WGTACW 2 cut(s) 954, 1021
TauI GCSGC 2 cut(s) 138, 992
TfiI GAWTC 1 cut(s) 504
Tru1I TTAA 4 cut(s) 744, 950, 984, 1065
Tru9I TTAA 4 cut(s) 744, 950, 984, 1065
TscAI CASTG 7 cut(s) 118, 129, 166, 394, 1128, 1155, 1223
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 5 cut(s) 271, 296, 945, 1114, 1277
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 6 cut(s) 45, 243, 633, 785, 790, 1341
TspRI CASTG 7 cut(s) 118, 129, 166, 394, 1128, 1155, 1223
VpaK11BI GGWCC 1 cut(s) 450
VspI ATTAAT 1 cut(s) 744
XapI RAATTY 4 cut(s) 39, 52, 87, 1181
XbaI TCTAGA 2 cut(s) 649, 1282
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 3 cut(s) 189, 650, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.