Rorug05G0484000

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014912
Physical Location & Seq
Reverse (-)
66626769 .. 66630537
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0484000.1

Sequence Viewer

Length: 540 bp
ATGAAGCCAAGCCTCCCCAGTCGCCAAACCCTAGCTCCGGCAAGCCTCCAAGGAGAATGTACCTCCGCCTACAGATCTAACTCGTCATCCTTTTTTATTCTCACAGACAGAGATTCCCAAATCCTCCCTCTTTCTATCAAGAATCGATTCACGACGACCCCTTTTTATCCCGATTCAATTTCCCCAGGTAGTGTGTTTGATTTTTGGGGGATGGCTGTGATGGTGCAGCAAATGGCTACGATAGTGGCCTTTTTTGGTGTATTGTCTTTCATATTCGGAATTATTGCGGAAAACAAGAAGATTTGCGACATAAATGAAGTATCAAGAGAGGAGGTGGATGAACTTACATCCGCCTATCAATATAGTACAGTGGGACATGGAGAAGTGCAATTTTATACAAATGACCTCAGCTTTGTTTTCCACATGATTCGCGGGCAGCAAAGACTTGGAAATGATACACCCAAGAACAACTTGACAAGGTGCACATTGAAGTGGCTGACTATTTGCAGACTTTGCTATAGATGGAGACTAGCAAAGTGA

Protein Analysis

179

Amino Acids

20.38

Weight (kDa)

8.63

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19770 AT5G19780
fragaria_vesca FvH4_3g45490
malus_domestica MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis pycom03g00450 pycom11g00410
rosa_chinensis RchiOBHm_Chr5g0083281
rosa_laevigata RLG00000037026
rosa_multiflora Rmu_sc0011792.1_g000008
rosa_roxburghii Rroxscaffold_1G00001210
rosa_rugosa Rorug05G0484000
rosa_samantha Rh5BG560700
rosa_wichuraiana Rw5G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 432
AciI CCGC 4 cut(s) 66, 287, 351, 432
AfaI GTAC 2 cut(s) 61, 367
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 2 cut(s) 177, 490
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 2 cut(s) 35, 411
AluI AGCT 2 cut(s) 35, 411
Alw21I GWGCWC 1 cut(s) 485
Alw26I GTCTC 1 cut(s) 520
Alw44I GTGCAC 1 cut(s) 481
AoxI GGCC 1 cut(s) 246
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 2 cut(s) 226, 436
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
Asp700I GAANNNNTTC 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 407
BbvI GCAGC 2 cut(s) 238, 448
BccI CCATC 3 cut(s) 205, 214, 516
BciT130I CCWGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 2 cut(s) 32, 530
BfmI CTRYAG 2 cut(s) 70, 517
BglII AGATCT 1 cut(s) 74
BisI GCNGC 2 cut(s) 227, 437
BlsI GCNGC 2 cut(s) 228, 438
Bme1390I CCNGG 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 186
BmrI ACTGGG 1 cut(s) 12
BmuI ACTGGG 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 145
BsaJI CCNNGG 2 cut(s) 49, 184
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 186
BseCI ATCGAT 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 49, 184
BseGI GGATG 4 cut(s) 86, 216, 343, 347
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 421
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 2 cut(s) 238, 448
BsgI GTGCAG 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 1 cut(s) 248
BshVI ATCGAT 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 485
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 520
BsmFI GGGAC 1 cut(s) 387
BsnI GGCC 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 1 cut(s) 74
BspACI CCGC 4 cut(s) 66, 287, 351, 432
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 420
BspDI ATCGAT 1 cut(s) 145
BspFNI CGCG 1 cut(s) 432
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 2 cut(s) 49, 184
BssMI GATC 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 370
BstAPI GCANNNNNTGC 1 cut(s) 513
BstC8I GCNNGC 2 cut(s) 43, 434
BstDEI CTNAG 1 cut(s) 407
BstF5I GGATG 4 cut(s) 86, 216, 343, 347
BstFNI CGCG 1 cut(s) 432
BstKTI GATC 1 cut(s) 77
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 1 cut(s) 74
BstMWI GCNNNNNNNGC 1 cut(s) 513
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 2 cut(s) 70, 517
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 1 cut(s) 432
BstV1I GCAGC 2 cut(s) 238, 448
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 145
BsuRI GGCC 1 cut(s) 248
BsuTUI ATCGAT 1 cut(s) 145
BtsCI GGATG 4 cut(s) 86, 216, 343, 347
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 2 cut(s) 43, 434
ClaI ATCGAT 1 cut(s) 145
Csp6I GTAC 2 cut(s) 60, 366
CviAII CATG 2 cut(s) 377, 424
CviJI RGCY 9 cut(s) 7, 12, 35, 45, 215, 236, 248, 411, 496
CviKI_1 RGCY 9 cut(s) 7, 12, 35, 45, 215, 236, 248, 411, 496
CviQI GTAC 2 cut(s) 60, 366
DdeI CTNAG 1 cut(s) 407
DpnI GATC 1 cut(s) 76
DpnII GATC 1 cut(s) 74
EciI GGCGGA 2 cut(s) 55, 340
Eco130I CCWWGG 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 380, 427
FaiI YATR 7 cut(s) 272, 311, 363, 378, 396, 425, 519
FalI AAGNNNNNCTT 2 cut(s) 455, 487
FaqI GGGAC 1 cut(s) 387
FatI CATG 2 cut(s) 376, 423
FauI CCCGC 1 cut(s) 425
Fnu4HI GCNGC 2 cut(s) 227, 437
FokI GGATG 4 cut(s) 73, 223, 334, 350
Fsp4HI GCNGC 2 cut(s) 227, 437
FspBI CTAG 2 cut(s) 32, 530
GluI GCNGC 2 cut(s) 227, 437
HaeIII GGCC 1 cut(s) 248
HapII CCGG 1 cut(s) 38
Hin1II CATG 2 cut(s) 380, 427
HinfI GANTC 5 cut(s) 113, 142, 147, 173, 427
HpaII CCGG 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 1 cut(s) 278
Hpy188III TCNNGA 4 cut(s) 139, 151, 170, 324
Hpy8I GTNNAC 1 cut(s) 483
Hpy99I CGWCG 1 cut(s) 157
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4V TGCA 4 cut(s) 226, 388, 483, 507
HpyF10VI GCNNNNNNNGC 1 cut(s) 513
HpyF3I CTNAG 1 cut(s) 407
Hsp92II CATG 2 cut(s) 380, 427
Kzo9I GATC 1 cut(s) 74
LmnI GCTCC 1 cut(s) 40
LpnPI CCDG 4 cut(s) 31, 51, 171, 198
Lsp1109I GCAGC 2 cut(s) 238, 448
MaeI CTAG 2 cut(s) 32, 530
MalI GATC 1 cut(s) 76
MboI GATC 1 cut(s) 74
MboII GAAGA 1 cut(s) 310
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 485
MluCI AATT 3 cut(s) 177, 279, 389
MnlI CCTC 8 cut(s) 23, 56, 73, 134, 138, 322, 325, 416
MroXI GAANNNNTTC 1 cut(s) 146
MslI CAYNNNNRTG 1 cut(s) 490
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 1 cut(s) 513
NdeII GATC 1 cut(s) 74
NlaIII CATG 2 cut(s) 380, 427
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 5 cut(s) 113, 142, 147, 173, 427
PkrI GCNGC 2 cut(s) 228, 438
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 74
RsaI GTAC 2 cut(s) 61, 367
RsaNI GTAC 2 cut(s) 60, 366
RseI CAYNNNNRTG 1 cut(s) 490
SatI GCNGC 2 cut(s) 227, 437
Sau3AI GATC 1 cut(s) 74
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 1 cut(s) 485
SetI ASST 7 cut(s) 37, 65, 190, 336, 408, 413, 482
SfcI CTRYAG 2 cut(s) 70, 517
SmiMI CAYNNNNRTG 1 cut(s) 490
Sse9I AATT 3 cut(s) 177, 279, 389
SsiI CCGC 4 cut(s) 66, 287, 351, 432
SspMI CTAG 2 cut(s) 32, 530
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 370
TaqI TCGA 1 cut(s) 145
TasI AATT 3 cut(s) 177, 279, 389
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 5 cut(s) 113, 142, 147, 173, 427
TscAI CASTG 1 cut(s) 375
TseI GCWGC 2 cut(s) 226, 436
TspDTI ATGAA 4 cut(s) 17, 259, 330, 354
TspRI CASTG 1 cut(s) 375
VneI GTGCAC 1 cut(s) 481
XmnI GAANNNNTTC 1 cut(s) 146
XspI CTAG 2 cut(s) 32, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.