MD11G1005100.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014912
Physical Location & Seq
Forward (+)
463833 .. 467284
3452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1005100.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGAGAGAAATTATAAGCATTCATATTGGGCAGGCTGGAATTCAGGTGGGCAATTCATGCTGGGAGCTCTACTGCCTCGAGCATGGAATTCAACCTGATGGGCAGATGCCCAGTGATACAACAGTGGGTAGCGGCCGTGATGCTTTCAATACCTTCTTCAGCGAGACTGGATCAGGCAAGCATGTCCCTAGGGCCATATTTGTCGATTTGGAACCAACTGTTATTGATGAAGTAAGGACTGGGTCTTACCGCCAACTCTTTCACCCCGAGCAGCTCATTTCTGGAAAGGAAGATGCTGCTAATAATTTTGCAAGAGGACACTATACAGTTGGAAAGGAAATTGTAGATCTCTGCCTTGATCGAGTTAGGAAATTGGCCGATAATTGCACTGGTTTACAAGGATTTTTGGTGTTTAATGCTGTGGGTGGTGGTACCGGTTCTGGTTTGGGGTCTTTGCTGTTAGAACGCTTGTCCGTGGATTATGGGAAGAAGTCAAAGCTTGGATTCACCATCTATCCTTCTCCGCAGGTTTCAACAGCAGTTGTCGAGCCTTATAATAGCGTTCTCTCTACTCATGCTCTCCTTGAACACACCGATGTATCTGTGCTCTTGGACAATGAAGCTATCTATGATATTTGCAGAAGATCTCTAGACATCGAGAGACCAACGTACACCAATTTGAATAGACTGATATCGCAAGTCATATCCTCCTTGACAACATCATTGAGATTTGATGGAGCCATTAACGTTGATGTTACAGAGTTCCAGACTAACCTTGTACCATATCCCCGCATTCATTTCATGCTTTCCTCATATGCTCCTGTTATCTCAGCTGCAAAGGCATACCATGAGCAGATCTCTATTCCTGAGATCACAAATGCAGTTTTTGAGCCCTCAAGCATGATGGCCAAGTGTGATCCCCGGCATGGAAAATACATGGCTTGCTGTCTAATGTACAGGGGTGATGTTGTTCCCAAGGATGTCAATGCTGCCGTTGCAACTATCAAAACAAAAAGGACTGTACAGTTTGTTGACTGGTGCCCAACAGGTTTCAAATGTGGCATAAACTATCAGCCTCCAACAGTTGTACCAGGGGGTGATCTTGCCAAGGTTCAGCGAGCAGTGTGCATGATCAGCAACAACACTGCTGTGGCTGAGGTGTTCTCACGCATTGACCATAAATTTGATCTCATGTATTCCAAGAGGGCATTTGTTCACTGGTATGTGGGTGAGGGAATGGAAGAAGGAGAGTTTTCAGAAGCTCGTGAAGATCTGGCTGCTCTAGAGAAAGATTACGAGGAAGTTGGTGCCGAAGGTGTGGATGATGAAGAGGGAAGTGAAGATTACTAG

Protein Analysis

450

Amino Acids

49.58

Weight (kDa)

5.04

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 7.7e-63 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 263 - 392 2.2e-51 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19770 AT5G19780
fragaria_vesca FvH4_3g45490
malus_domestica MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis pycom03g00450 pycom11g00410
rosa_chinensis RchiOBHm_Chr5g0083281
rosa_laevigata RLG00000037026
rosa_multiflora Rmu_sc0011792.1_g000008
rosa_roxburghii Rroxscaffold_1G00001210
rosa_rugosa Rorug05G0484000
rosa_samantha Rh5BG560700
rosa_wichuraiana Rw5G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 14, 555
Acc36I ACCTGC 1 cut(s) 517
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 3 cut(s) 431, 1038, 1307
AciI CCGC 4 cut(s) 132, 250, 524, 790
AclI AACGTT 1 cut(s) 747
AclWI GGATC 2 cut(s) 178, 911
AcoI YGGCCR 3 cut(s) 133, 375, 906
AcsI RAATTY 3 cut(s) 39, 87, 1181
AcuI CTGAAG 1 cut(s) 142
AfaI GTAC 6 cut(s) 433, 671, 780, 956, 1023, 1089
AfiI CCNNNNNNNGG 1 cut(s) 926
AgeI ACCGGT 1 cut(s) 434
AgsI TTSAA 6 cut(s) 92, 148, 534, 587, 682, 1054
AjnI CCWGG 1 cut(s) 1090
AleI CACNNNNGTG 1 cut(s) 1148
AluBI AGCT 6 cut(s) 67, 274, 499, 623, 833, 1262
AluI AGCT 6 cut(s) 67, 274, 499, 623, 833, 1262
Alw21I GWGCWC 2 cut(s) 69, 609
Alw26I GTCTC 2 cut(s) 158, 655
AlwI GGATC 2 cut(s) 178, 911
Ama87I CYCGRG 2 cut(s) 77, 266
AoxI GGCC 4 cut(s) 133, 192, 375, 906
ApeKI GCWGC 5 cut(s) 271, 296, 833, 989, 1277
ApoI RAATTY 3 cut(s) 39, 87, 1181
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AsiGI ACCGGT 1 cut(s) 434
Asp718I GGTACC 1 cut(s) 431
AspA2I CCTAGG 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 192
AsuC2I CCSGG 1 cut(s) 922
AsuHPI GGTGA 5 cut(s) 254, 499, 974, 1109, 1241
AvaI CYCGRG 2 cut(s) 77, 266
AvrII CCTAGG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 1043
BaeI ACNNNNGTAYC 6 cut(s) 108, 141, 582, 615, 1071, 1104
BalI TGGCCA 1 cut(s) 908
BanI GGYRCC 3 cut(s) 431, 1038, 1307
BanII GRGCYC 2 cut(s) 69, 894
BauI CACGAG 1 cut(s) 1263
Bbv12I GWGCWC 2 cut(s) 69, 609
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 5 cut(s) 283, 283, 820, 976, 1264
BccI CCATC 4 cut(s) 92, 518, 728, 898
BceAI ACGGC 2 cut(s) 120, 977
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 1 cut(s) 1092
BclI TGATCA 1 cut(s) 1131
BcnI CCSGG 1 cut(s) 922
BcoDI GTCTC 2 cut(s) 158, 655
BfaI CTAG 4 cut(s) 189, 650, 1283, 1348
BfuAI ACCTGC 1 cut(s) 517
BglII AGATCT 4 cut(s) 346, 644, 855, 1270
BisI GCNGC 6 cut(s) 133, 272, 297, 834, 990, 1278
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 6 cut(s) 134, 273, 298, 835, 991, 1279
Bme1390I CCNGG 2 cut(s) 922, 1092
BmeT110I CYCGRG 2 cut(s) 77, 266
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 5 cut(s) 213, 433, 739, 1040, 1309
BmrFI CCNGG 2 cut(s) 922, 1092
BmrI ACTGGG 2 cut(s) 105, 249
BmsI GCATC 3 cut(s) 96, 130, 283
BmuI ACTGGG 2 cut(s) 105, 249
BplI GAGNNNNNCTC 4 cut(s) 842, 874, 1148, 1180
Bpu10I CCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 1 cut(s) 880
BpuMI CCSGG 1 cut(s) 922
BsaI GGTCTC 1 cut(s) 655
BsaJI CCNNGG 6 cut(s) 188, 474, 920, 975, 1091, 1107
BsaWI WCCGGW 1 cut(s) 434
Bsc4I CCNNNNNNNGG 1 cut(s) 926
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 6 cut(s) 111, 172, 244, 394, 1040, 1223
BseBI CCWGG 1 cut(s) 1092
BseDI CCNNGG 6 cut(s) 188, 474, 920, 975, 1091, 1107
BseGI GGATG 2 cut(s) 985, 1327
BseLI CCNNNNNNNGG 1 cut(s) 926
BseMII CTCAG 3 cut(s) 843, 858, 1146
BseNI ACTGG 6 cut(s) 111, 172, 244, 394, 1040, 1223
BseSI GKGCMC 1 cut(s) 1043
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 5 cut(s) 283, 283, 820, 976, 1264
BseYI CCCAGC 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 4 cut(s) 135, 194, 377, 908
BshNI GGYRCC 3 cut(s) 431, 1038, 1307
BshTI ACCGGT 1 cut(s) 434
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 2 cut(s) 69, 609
BsiHKCI CYCGRG 2 cut(s) 77, 266
BsiSI CCGG 2 cut(s) 435, 922
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 926
BsmAI GTCTC 2 cut(s) 158, 655
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 2 cut(s) 18, 792
BsnI GGCC 4 cut(s) 135, 194, 377, 908
Bso31I GGTCTC 1 cut(s) 655
BsoBI CYCGRG 2 cut(s) 77, 266
Bsp1286I GDGCHC 4 cut(s) 69, 609, 894, 1043
Bsp1407I TGTACA 2 cut(s) 954, 1021
BspACI CCGC 4 cut(s) 132, 250, 524, 790
BspANI GGCC 4 cut(s) 135, 194, 377, 908
BspCNI CTCAG 3 cut(s) 842, 859, 1147
BspLI GGNNCC 5 cut(s) 213, 433, 739, 1040, 1309
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 2 cut(s) 178, 911
BspT107I GGYRCC 3 cut(s) 431, 1038, 1307
BspTNI GGTCTC 1 cut(s) 655
BsrFI RCCGGY 1 cut(s) 434
BsrGI TGTACA 2 cut(s) 954, 1021
BsrI ACTGG 6 cut(s) 111, 172, 244, 394, 1040, 1223
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 6 cut(s) 188, 474, 920, 975, 1091, 1107
BssSI CACGAG 1 cut(s) 1263
BssT1I CCWWGG 3 cut(s) 188, 975, 1107
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 1 cut(s) 1092
Bst4CI ACNGT 6 cut(s) 124, 220, 328, 1021, 1026, 1084
Bst6I CTCTTC 1 cut(s) 1323
BstAPI GCANNNNNTGC 1 cut(s) 57
BstAUI TGTACA 2 cut(s) 954, 1021
BstC8I GCNNGC 4 cut(s) 33, 179, 943, 1119
BstDEI CTNAG 3 cut(s) 829, 867, 1155
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 2 cut(s) 985, 1327
BstMAI GTCTC 2 cut(s) 158, 655
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 4 cut(s) 57, 839, 995, 1134
BstNI CCWGG 1 cut(s) 1092
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 920, 1090
BstSLI GKGCMC 1 cut(s) 1043
BstV1I GCAGC 5 cut(s) 283, 283, 820, 976, 1264
BstX2I RGATCY 4 cut(s) 346, 644, 855, 1270
BstYI RGATCY 4 cut(s) 346, 644, 855, 1270
BstZI CGGCCG 1 cut(s) 133
BsuRI GGCC 4 cut(s) 135, 194, 377, 908
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 2 cut(s) 985, 1327
BtsI GCAGTG 2 cut(s) 1128, 1143
BtsIMutI CAGTG 6 cut(s) 118, 129, 387, 1128, 1143, 1216
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 4 cut(s) 33, 179, 943, 1119
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 192
Csp6I GTAC 6 cut(s) 432, 670, 779, 955, 1022, 1088
CspAI ACCGGT 1 cut(s) 434
CviQI GTAC 6 cut(s) 432, 670, 779, 955, 1022, 1088
DdeI CTNAG 3 cut(s) 829, 867, 1155
EaeI YGGCCR 3 cut(s) 133, 375, 906
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 1323
EarI CTCTTC 1 cut(s) 1323
Ecl136II GAGCTC 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 3 cut(s) 188, 975, 1107
Eco24I GRGCYC 2 cut(s) 69, 894
Eco31I GGTCTC 1 cut(s) 655
Eco32I GATATC 1 cut(s) 693
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 142
Eco88I CYCGRG 2 cut(s) 77, 266
EcoICRI GAGCTC 1 cut(s) 67
EcoRI GAATTC 2 cut(s) 39, 87
EcoRII CCWGG 1 cut(s) 1090
EcoRV GATATC 1 cut(s) 693
EcoT14I CCWWGG 3 cut(s) 188, 975, 1107
EcoT38I GRGCYC 2 cut(s) 69, 894
ErhI CCWWGG 3 cut(s) 188, 975, 1107
FaqI GGGAC 1 cut(s) 170
FauI CCCGC 1 cut(s) 797
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 1 cut(s) 1131
Fnu4HI GCNGC 6 cut(s) 133, 272, 297, 834, 990, 1278
FokI GGATG 2 cut(s) 992, 1334
FriOI GRGCYC 2 cut(s) 69, 894
Fsp4HI GCNGC 6 cut(s) 133, 272, 297, 834, 990, 1278
FspBI CTAG 4 cut(s) 189, 650, 1283, 1348
GluI GCNGC 6 cut(s) 133, 272, 297, 834, 990, 1278
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 4 cut(s) 135, 194, 377, 908
HapII CCGG 2 cut(s) 435, 922
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 504
HpaII CCGG 2 cut(s) 435, 922
HphI GGTGA 5 cut(s) 254, 499, 974, 1109, 1241
Hpy166II GTNNAC 4 cut(s) 395, 672, 1033, 1216
Hpy188I TCNGA 1 cut(s) 1258
Hpy188III TCNNGA 7 cut(s) 282, 650, 658, 766, 866, 1265, 1283
Hpy8I GTNNAC 4 cut(s) 395, 672, 1033, 1216
HpyAV CCTTC 4 cut(s) 163, 528, 1238, 1307
HpyCH4III ACNGT 6 cut(s) 124, 220, 328, 1021, 1026, 1084
HpyCH4IV ACGT 2 cut(s) 668, 747
HpyCH4V TGCA 7 cut(s) 311, 387, 639, 836, 881, 998, 1128
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 839, 995, 1134
HpyF3I CTNAG 3 cut(s) 829, 867, 1155
HpySE526I ACGT 2 cut(s) 668, 747
KpnI GGTACC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 1131
LmnI GCTCC 3 cut(s) 64, 737, 823
Lsp1109I GCAGC 5 cut(s) 283, 283, 820, 976, 1264
LweI GCATC 3 cut(s) 96, 130, 283
MaeI CTAG 4 cut(s) 189, 650, 1283, 1348
MaeII ACGT 2 cut(s) 668, 747
MaeIII GTNAC 1 cut(s) 754
MboII GAAGA 7 cut(s) 148, 302, 499, 654, 1253, 1280, 1340
MflI RGATCY 4 cut(s) 346, 644, 855, 1270
MhlI GDGCHC 4 cut(s) 69, 609, 894, 1043
MlsI TGGCCA 1 cut(s) 908
MluNI TGGCCA 1 cut(s) 908
MmeI TCCRAC 2 cut(s) 310, 1103
Mox20I TGGCCA 1 cut(s) 908
MscI TGGCCA 1 cut(s) 908
MseI TTAA 2 cut(s) 414, 744
MslI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
Msp20I TGGCCA 1 cut(s) 908
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 2 cut(s) 435, 922
MspR9I CCNGG 2 cut(s) 922, 1092
Mva1269I GAATGC 2 cut(s) 18, 792
MvaI CCWGG 1 cut(s) 1092
MwoI GCNNNNNNNGC 4 cut(s) 57, 839, 995, 1134
NciI CCSGG 1 cut(s) 922
NdeI CATATG 1 cut(s) 814
NlaIV GGNNCC 5 cut(s) 213, 433, 739, 1040, 1309
NspI RCATGY 1 cut(s) 185
OliI CACNNNNGTG 1 cut(s) 1148
PaeR7I CTCGAG 1 cut(s) 77
PctI GAATGC 2 cut(s) 18, 792
PfeI GAWTC 1 cut(s) 504
PflFI GACNNNGTC 1 cut(s) 241
PinAI ACCGGT 1 cut(s) 434
PkrI GCNGC 6 cut(s) 134, 273, 298, 835, 991, 1279
PsiI TTATAA 2 cut(s) 14, 555
Psp124BI GAGCTC 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 1090
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 1090
PspN4I GGNNCC 5 cut(s) 213, 433, 739, 1040, 1309
PspPI GGNCC 1 cut(s) 192
PspXI VCTCGAGB 1 cut(s) 77
PsuI RGATCY 4 cut(s) 346, 644, 855, 1270
PsyI GACNNNGTC 1 cut(s) 241
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 6 cut(s) 433, 671, 780, 956, 1023, 1089
RsaNI GTAC 6 cut(s) 432, 670, 779, 955, 1022, 1088
RseI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 414, 744
SatI GCNGC 6 cut(s) 133, 272, 297, 834, 990, 1278
Sau96I GGNCC 1 cut(s) 192
ScrFI CCNGG 2 cut(s) 922, 1092
SduI GDGCHC 4 cut(s) 69, 609, 894, 1043
SfaNI GCATC 3 cut(s) 96, 130, 283
Sfr274I CTCGAG 1 cut(s) 77
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SmlI CTYRAG 2 cut(s) 77, 895
SmoI CTYRAG 2 cut(s) 77, 895
SsiI CCGC 4 cut(s) 132, 250, 524, 790
SspMI CTAG 4 cut(s) 189, 650, 1283, 1348
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 920, 1090
StyI CCWWGG 3 cut(s) 188, 975, 1107
TaaI ACNGT 6 cut(s) 124, 220, 328, 1021, 1026, 1084
TaiI ACGT 2 cut(s) 671, 750
TaqI TCGA 5 cut(s) 78, 204, 361, 546, 657
TatI WGTACW 2 cut(s) 954, 1021
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 504
Tru1I TTAA 2 cut(s) 414, 744
Tru9I TTAA 2 cut(s) 414, 744
TscAI CASTG 6 cut(s) 118, 129, 394, 1128, 1150, 1223
TseI GCWGC 5 cut(s) 271, 296, 833, 989, 1277
TspDTI ATGAA 7 cut(s) 11, 45, 243, 633, 785, 790, 1341
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 6 cut(s) 118, 129, 394, 1128, 1150, 1223
Tth111I GACNNNGTC 1 cut(s) 241
XapI RAATTY 3 cut(s) 39, 87, 1181
XbaI TCTAGA 2 cut(s) 649, 1282
XceI RCATGY 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 77
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 4 cut(s) 189, 650, 1283, 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.