RchiOBHm_Chr5g0083281

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014912
Physical Location & Seq
Reverse (-)
88948331 .. 88951213
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35743

Sequence Viewer

Length: 1359 bp
ATGAGAGAGATCATAAGCATTCATATTGGGCAGGCAGGTATTCAGGTGGGAAATTCATGCTGGGAGCTCTATTGTCTTGAGCATGGAATTCAGCCTGATGGTCAGATGCCTAGTGACAACACTATAGGTGTTGGAAGGGATGCTTTCAATACCTTCTTCAGTGAGACTGGATCAGGCAAGCATGTCCCTAGGGCCATATTTGTTGATCTGGAACCAACTGTTATTGATGAAGTTAGGACAGGCTCTTACCGCCAACTATTCCACCCCGAGCAGCTCATTTCTGGTAAAGAAGATGCCGCTAATAACTTTGCAAGAGGACATTATACAGTTGGAAAGGAAATTGTGGATCTCTGCCTTGATCGAGTGAGGAAATTGGCCGACAATTGCACTGGTTTACAAGGGTTTTTGGTGTTCAATGCTGTTGGTGGTGGTACCGGTTCTGGTCTGGGGTCCTTGTTGTTAGAACGTTTGTCTGTGGATTATGGGAAAAAGTCAAAGCTTGGATTCACCATCTATCCTTCTCCCCAGGTTTCAACAGCAGTTGTCGAGCCTTACAACAGCGTTCTTTCTACTCATGCCCTCCTTGAACACACAGATGTATCTGTGCTCTTGGACAATGAAGCTATCTACGACATTTGCAGGAGATCCCTAGACATTGAGAGACCAACTTATACCAATTTGAACAGATTGATTTCACAAGTCATTTCATCCTTGACAACATCATTGAGATTTGATGGAGCCATCAATGTAGATATCACAGAGTTCCAGACTAACCTTGTCCCGTATCCACGTATCCATTTCATGCTCTCCTCATATGCTCCTGTCATCTCAGCTGCAAAGGCATACCATGAGCAGATCTCTGTTCCTGAGATCACCAATGCAGTTTTTGAGCCCTCAAGCATGATGGCGAAGTGTGATCCCAGGCATGGCAAGTACATGGCGTGCTGCTTAATGTACAGGGGAGATGTTGTTCCCAAGGACGTTAACGCTGCTGTTGCAACTATCAAAACAAAAAGGACTGTACAATTTGTTGACTGGTGCCCAACTGGCTTCAAGTGCGGTATTAACTATGAACCTCCAACAGTTGTACCTGGGGGTGATCTGGCCAAGGTGCAGCGAGCAGTTTGCATGATCAGCAACAACACAGCAGTGGCTGAGGTGTTCTCACGCATTGACCACAAGTTTGATCTCATGTACTCAAAGAGGGCTTTCGTCCACTGGTATGTTGGTGAAGGCATGGAAGAAGGAGAGTTCTCAGAAGCCCGTGAAGATCTGGCTGCACTTGAGAAAGATTACGAGGAAGTTGGTGCTGAAGGTGTGGATGATGAAGAGGGGGGAAGTGAGGATTACGAGAATTGA

Protein Analysis

452

Amino Acids

49.92

Weight (kDa)

4.96

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 8.3e-63 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 263 - 392 1.4e-51 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19770 AT5G19780
fragaria_vesca FvH4_3g45490
malus_domestica MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis pycom03g00450 pycom11g00410
rosa_chinensis RchiOBHm_Chr5g0083281
rosa_laevigata RLG00000037026
rosa_multiflora Rmu_sc0011792.1_g000008
rosa_roxburghii Rroxscaffold_1G00001210
rosa_rugosa Rorug05G0484000
rosa_samantha Rh5BG560700
rosa_wichuraiana Rw5G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 26
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 2 cut(s) 431, 1038
AciI CCGC 3 cut(s) 250, 297, 1059
AclI AACGTT 1 cut(s) 466
AclWI GGATC 4 cut(s) 178, 354, 639, 911
AcoI YGGCCR 2 cut(s) 375, 1104
AcsI RAATTY 2 cut(s) 52, 87
AcuI CTGAAG 2 cut(s) 142, 1332
AfaI GTAC 6 cut(s) 433, 935, 956, 1023, 1089, 1196
AfiI CCNNNNNNNGG 1 cut(s) 926
AgeI ACCGGT 1 cut(s) 434
AgsI TTSAA 6 cut(s) 148, 415, 534, 587, 682, 1054
AjnI CCWGG 3 cut(s) 525, 920, 1090
AleI CACNNNNGTG 1 cut(s) 1148
AloI GAACNNNNNNTCC 2 cut(s) 954, 986
AluBI AGCT 5 cut(s) 67, 274, 499, 623, 833
AluI AGCT 5 cut(s) 67, 274, 499, 623, 833
Alw21I GWGCWC 2 cut(s) 69, 609
Alw26I GTCTC 2 cut(s) 158, 655
AlwI GGATC 4 cut(s) 178, 354, 639, 911
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 3 cut(s) 192, 375, 1104
ApeKI GCWGC 6 cut(s) 271, 833, 945, 989, 1114, 1277
ApoI RAATTY 2 cut(s) 52, 87
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AsiGI ACCGGT 1 cut(s) 434
Asp718I GGTACC 1 cut(s) 431
AspA2I CCTAGG 1 cut(s) 188
AspS9I GGNCC 2 cut(s) 192, 450
AsuHPI GGTGA 4 cut(s) 499, 865, 1109, 1241
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 1043
BaeI ACNNNNGTAYC 4 cut(s) 582, 615, 1071, 1104
BalI TGGCCA 1 cut(s) 1106
BanI GGYRCC 2 cut(s) 431, 1038
BanII GRGCYC 2 cut(s) 69, 894
Bbv12I GWGCWC 2 cut(s) 69, 609
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 6 cut(s) 283, 820, 932, 976, 1126, 1264
BccI CCATC 5 cut(s) 92, 518, 728, 749, 898
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 3 cut(s) 527, 922, 1092
BciVI GTATCC 2 cut(s) 795, 803
BclI TGATCA 1 cut(s) 1131
BcoDI GTCTC 2 cut(s) 158, 655
BfaI CTAG 3 cut(s) 111, 189, 650
BfmI CTRYAG 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 26
BfuI GTATCC 2 cut(s) 795, 803
BglI GCCNNNNNGGC 1 cut(s) 1047
BglII AGATCT 2 cut(s) 855, 1270
BisI GCNGC 7 cut(s) 272, 297, 834, 946, 990, 1115, 1278
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 7 cut(s) 273, 298, 835, 947, 991, 1116, 1279
Bme1390I CCNGG 3 cut(s) 527, 922, 1092
Bme18I GGWCC 1 cut(s) 450
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 2 cut(s) 192, 450
BmiI GGNNCC 5 cut(s) 213, 433, 451, 739, 1040
BmrFI CCNGG 3 cut(s) 527, 922, 1092
BmsI GCATC 3 cut(s) 96, 130, 283
BplI GAGNNNNNCTC 4 cut(s) 842, 874, 1148, 1180
Bpu10I CCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 3 cut(s) 98, 880, 1304
BsaAI YACGTR 1 cut(s) 791
BsaI GGTCTC 1 cut(s) 655
BsaJI CCNNGG 6 cut(s) 188, 525, 920, 975, 1091, 1107
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 1 cut(s) 926
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 5 cut(s) 172, 394, 1040, 1051, 1223
BseBI CCWGG 3 cut(s) 527, 922, 1092
BseDI CCNNGG 6 cut(s) 188, 525, 920, 975, 1091, 1107
BseGI GGATG 3 cut(s) 145, 707, 1327
BseLI CCNNNNNNNGG 1 cut(s) 926
BseMII CTCAG 4 cut(s) 843, 858, 1146, 1269
BseNI ACTGG 5 cut(s) 172, 394, 1040, 1051, 1223
BseRI GAGGAG 1 cut(s) 799
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 6 cut(s) 283, 820, 932, 976, 1126, 1264
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 2 cut(s) 1133, 1263
BshFI GGCC 3 cut(s) 194, 377, 1106
BshNI GGYRCC 2 cut(s) 431, 1038
BshTI ACCGGT 1 cut(s) 434
BsiHKAI GWGCWC 2 cut(s) 69, 609
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 2 cut(s) 170, 764
BslI CCNNNNNNNGG 1 cut(s) 926
BsmAI GTCTC 2 cut(s) 158, 655
BsmFI GGGAC 2 cut(s) 170, 764
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 3 cut(s) 194, 377, 1106
Bso31I GGTCTC 1 cut(s) 655
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 4 cut(s) 69, 609, 894, 1043
Bsp1407I TGTACA 2 cut(s) 954, 1021
BspACI CCGC 3 cut(s) 250, 297, 1059
BspANI GGCC 3 cut(s) 194, 377, 1106
BspCNI CTCAG 4 cut(s) 842, 859, 1147, 1268
BspLI GGNNCC 5 cut(s) 213, 433, 451, 739, 1040
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 4 cut(s) 178, 354, 639, 911
BspT107I GGYRCC 2 cut(s) 431, 1038
BspTNI GGTCTC 1 cut(s) 655
BsrFI RCCGGY 1 cut(s) 434
BsrGI TGTACA 2 cut(s) 954, 1021
BsrI ACTGG 5 cut(s) 172, 394, 1040, 1051, 1223
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 6 cut(s) 188, 525, 920, 975, 1091, 1107
BssT1I CCWWGG 3 cut(s) 188, 975, 1107
Bst2UI CCWGG 3 cut(s) 527, 922, 1092
Bst4CI ACNGT 4 cut(s) 220, 328, 1021, 1084
Bst6I CTCTTC 1 cut(s) 1323
BstAUI TGTACA 2 cut(s) 954, 1021
BstBAI YACGTR 1 cut(s) 791
BstC8I GCNNGC 4 cut(s) 33, 179, 943, 1119
BstDEI CTNAG 4 cut(s) 829, 867, 1155, 1255
BstF5I GGATG 3 cut(s) 145, 707, 1327
BstMAI GTCTC 2 cut(s) 158, 655
BstMWI GCNNNNNNNGC 6 cut(s) 249, 839, 995, 1047, 1056, 1134
BstNI CCWGG 3 cut(s) 527, 922, 1092
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 3 cut(s) 525, 920, 1090
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 1043
BstV1I GCAGC 6 cut(s) 283, 820, 932, 976, 1126, 1264
BstX2I RGATCY 4 cut(s) 346, 644, 855, 1270
BstYI RGATCY 4 cut(s) 346, 644, 855, 1270
BsuI GTATCC 2 cut(s) 795, 803
BsuRI GGCC 3 cut(s) 194, 377, 1106
BtsCI GGATG 3 cut(s) 145, 707, 1327
BtsI GCAGTG 1 cut(s) 1155
BtsIMutI CAGTG 4 cut(s) 166, 387, 1155, 1216
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 4 cut(s) 33, 179, 943, 1119
CaiI CAGNNNCTG 1 cut(s) 1154
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 192, 450
Csp6I GTAC 6 cut(s) 432, 934, 955, 1022, 1088, 1195
CspAI ACCGGT 1 cut(s) 434
CviQI GTAC 6 cut(s) 432, 934, 955, 1022, 1088, 1195
DdeI CTNAG 4 cut(s) 829, 867, 1155, 1255
EaeI YGGCCR 2 cut(s) 375, 1104
Eam1104I CTCTTC 1 cut(s) 1323
EarI CTCTTC 1 cut(s) 1323
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 3 cut(s) 188, 975, 1107
Eco24I GRGCYC 2 cut(s) 69, 894
Eco31I GGTCTC 1 cut(s) 655
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 142, 1332
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 450
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 3 cut(s) 525, 920, 1090
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 3 cut(s) 188, 975, 1107
EcoT38I GRGCYC 2 cut(s) 69, 894
ErhI CCWWGG 3 cut(s) 188, 975, 1107
FalI AAGNNNNNCTT 2 cut(s) 127, 159
FaqI GGGAC 2 cut(s) 170, 764
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 1 cut(s) 1131
Fnu4HI GCNGC 7 cut(s) 272, 297, 834, 946, 990, 1115, 1278
FokI GGATG 3 cut(s) 152, 694, 1334
FriOI GRGCYC 2 cut(s) 69, 894
Fsp4HI GCNGC 7 cut(s) 272, 297, 834, 946, 990, 1115, 1278
FspBI CTAG 3 cut(s) 111, 189, 650
GluI GCNGC 7 cut(s) 272, 297, 834, 946, 990, 1115, 1278
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 3 cut(s) 194, 377, 1106
HapII CCGG 1 cut(s) 435
HincII GTYRAC 2 cut(s) 985, 1033
HindII GTYRAC 2 cut(s) 985, 1033
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 504
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 1 cut(s) 435
HphI GGTGA 4 cut(s) 499, 865, 1109, 1241
Hpy166II GTNNAC 4 cut(s) 395, 985, 1033, 1216
Hpy188I TCNGA 2 cut(s) 105, 1258
Hpy188III TCNNGA 4 cut(s) 77, 209, 766, 866
Hpy8I GTNNAC 4 cut(s) 395, 985, 1033, 1216
HpyAV CCTTC 6 cut(s) 129, 163, 528, 1226, 1238, 1307
HpyCH4III ACNGT 4 cut(s) 220, 328, 1021, 1084
HpyCH4IV ACGT 3 cut(s) 466, 790, 981
HpyCH4V TGCA 9 cut(s) 311, 387, 639, 836, 881, 998, 1114, 1128, 1280
HpyF10VI GCNNNNNNNGC 6 cut(s) 249, 839, 995, 1047, 1056, 1134
HpyF3I CTNAG 4 cut(s) 829, 867, 1155, 1255
HpySE526I ACGT 3 cut(s) 466, 790, 981
KpnI GGTACC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 1131
KspAI GTTAAC 1 cut(s) 985
LmnI GCTCC 3 cut(s) 64, 737, 823
Lsp1109I GCAGC 6 cut(s) 283, 820, 932, 976, 1126, 1264
LweI GCATC 3 cut(s) 96, 130, 283
MaeI CTAG 3 cut(s) 111, 189, 650
MaeII ACGT 3 cut(s) 466, 790, 981
MaeIII GTNAC 1 cut(s) 113
MboII GAAGA 5 cut(s) 148, 302, 1253, 1280, 1340
MfeI CAATTG 1 cut(s) 382
MflI RGATCY 4 cut(s) 346, 644, 855, 1270
MhlI GDGCHC 4 cut(s) 69, 609, 894, 1043
MlsI TGGCCA 1 cut(s) 1106
MluCI AATT 8 cut(s) 52, 87, 339, 371, 382, 676, 1025, 1354
MluNI TGGCCA 1 cut(s) 1106
MmeI TCCRAC 3 cut(s) 112, 310, 1103
Mox20I TGGCCA 1 cut(s) 1106
MscI TGGCCA 1 cut(s) 1106
MseI TTAA 3 cut(s) 950, 984, 1065
MslI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
Msp20I TGGCCA 1 cut(s) 1106
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 3 cut(s) 527, 922, 1092
MunI CAATTG 1 cut(s) 382
Mva1269I GAATGC 1 cut(s) 18
MvaI CCWGG 3 cut(s) 527, 922, 1092
MwoI GCNNNNNNNGC 6 cut(s) 249, 839, 995, 1047, 1056, 1134
NdeI CATATG 1 cut(s) 814
NlaIV GGNNCC 5 cut(s) 213, 433, 451, 739, 1040
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 1 cut(s) 185
OliI CACNNNNGTG 1 cut(s) 1148
PctI GAATGC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 504
PinAI ACCGGT 1 cut(s) 434
PkrI GCNGC 7 cut(s) 273, 298, 835, 947, 991, 1116, 1279
Ppu21I YACGTR 1 cut(s) 791
PpuMI RGGWCCY 1 cut(s) 450
Psp124BI GAGCTC 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 3 cut(s) 525, 920, 1090
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 3 cut(s) 525, 920, 1090
PspN4I GGNNCC 5 cut(s) 213, 433, 451, 739, 1040
PspPI GGNCC 2 cut(s) 192, 450
PspPPI RGGWCCY 1 cut(s) 450
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 4 cut(s) 346, 644, 855, 1270
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 6 cut(s) 433, 935, 956, 1023, 1089, 1196
RsaNI GTAC 6 cut(s) 432, 934, 955, 1022, 1088, 1195
RseI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 3 cut(s) 950, 984, 1065
SatI GCNGC 7 cut(s) 272, 297, 834, 946, 990, 1115, 1278
Sau96I GGNCC 2 cut(s) 192, 450
ScrFI CCNGG 3 cut(s) 527, 922, 1092
SduI GDGCHC 4 cut(s) 69, 609, 894, 1043
SfaNI GCATC 3 cut(s) 96, 130, 283
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 3 cut(s) 594, 1148, 1221
SmlI CTYRAG 3 cut(s) 77, 895, 1283
SmoI CTYRAG 3 cut(s) 77, 895, 1283
Sse9I AATT 8 cut(s) 52, 87, 339, 371, 382, 676, 1025, 1354
SsiI CCGC 3 cut(s) 250, 297, 1059
SspMI CTAG 3 cut(s) 111, 189, 650
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 3 cut(s) 525, 920, 1090
StyI CCWWGG 3 cut(s) 188, 975, 1107
TaaI ACNGT 4 cut(s) 220, 328, 1021, 1084
TaiI ACGT 3 cut(s) 469, 793, 984
TaqI TCGA 2 cut(s) 361, 546
TasI AATT 8 cut(s) 52, 87, 339, 371, 382, 676, 1025, 1354
TatI WGTACW 4 cut(s) 933, 954, 1021, 1194
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 1 cut(s) 504
Tru1I TTAA 3 cut(s) 950, 984, 1065
Tru9I TTAA 3 cut(s) 950, 984, 1065
TscAI CASTG 4 cut(s) 166, 394, 1155, 1223
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 6 cut(s) 271, 833, 945, 989, 1114, 1277
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 8 cut(s) 11, 45, 243, 633, 696, 790, 1086, 1341
TspRI CASTG 4 cut(s) 166, 394, 1155, 1223
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 2 cut(s) 52, 87
XceI RCATGY 1 cut(s) 185
XcmI CCANNNNNNNNNTGG 1 cut(s) 1223
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 3 cut(s) 111, 189, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.