pycom03g00450

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014912
Physical Location & Seq
Forward (+)
378772 .. 380961
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00450.4

Sequence Viewer

Length: 870 bp
ATGTCTAGGGTGGGCAATTCATGCTGGGAGCTCTACTGCCTCGAGCATGGAATTCAACCTGATGGCCAGATGCCCAGTGATACCACAGTGGGCAGTGGCCACGATGCTTTCAACACCTTCTTCAGCGAGACTGGAGCAGGCAAGCATGTCCCAAGAGCCATATTTGTTGATCTCGAACCAACTGTCATTGATGAAGTTAGGACTGGGACTTACCGCCAACTCTTCCACCCTGAGCAGCTCATTTCTGGAAAGGAAGATGCTGCTAATAATTTCGCAAGAGGACACTATACAGTTGGAAAGGAAATTGTAGATCTCTGTCTTGATCGAGTTAGGAAATTGGCCGATAATTGCACTGGTTTACAAGGATTTCTGGTGTTCAATGCTGTTGGTGGTGGTACTGGTTCTGGTTTGGGGTCCTTGCTCTTAGAACGCTTGTCGGTGGATTATGGAAAGAAGTCAAAGCTTGGGTTCACCATCTATCCTTCTCCGCAGGTTTCAACAGCAGTTGTTGAGCCTTATAATAGCGTTCTCTCTACTCATGCCCTCCTTGAACACACAGATGTATCTGTGCTCTTGGACAATGAAGCTATCTATGATATTTGCTGGAGATCTCTAGACATCGAGAGACCAACTTACACCAATTTGAATAGACTGATATCACAAGTCATATCCTCCTTGACAACATCATTGAGATTTGATGGAGCCATTAATGTTGATGTTACAGAGTTCCAGACAAACCTCGTTCCATACCCCCGCATCCATTTCATGCTTTCCTCATATGCCCCTGTTATCTCAGCTGCAAAGGCATACCATGAGCAGATCGATCAATCCCTGAGATCACAAATGCAGTTTTTGAACCCTCAAGCATGA

Protein Analysis

290

Amino Acids

31.92

Weight (kDa)

5.32

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19770 AT5G19780
fragaria_vesca FvH4_3g45490
malus_domestica MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis pycom03g00450 pycom11g00410
rosa_chinensis RchiOBHm_Chr5g0083281
rosa_laevigata RLG00000037026
rosa_multiflora Rmu_sc0011792.1_g000008
rosa_roxburghii Rroxscaffold_1G00001210
rosa_rugosa Rorug05G0484000
rosa_samantha Rh5BG560700
rosa_wichuraiana Rw5G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
Acc36I ACCTGC 1 cut(s) 481
AciI CCGC 3 cut(s) 214, 488, 754
AcoI YGGCCR 3 cut(s) 64, 97, 339
AcsI RAATTY 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 106
AfaI GTAC 1 cut(s) 397
AgsI TTSAA 7 cut(s) 56, 112, 379, 498, 551, 646, 856
AluBI AGCT 5 cut(s) 31, 238, 463, 587, 797
AluI AGCT 5 cut(s) 31, 238, 463, 587, 797
Alw21I GWGCWC 2 cut(s) 33, 573
Alw26I GTCTC 2 cut(s) 122, 619
Ama87I CYCGRG 1 cut(s) 41
AoxI GGCC 3 cut(s) 64, 97, 339
ApeKI GCWGC 3 cut(s) 235, 260, 797
ApoI RAATTY 1 cut(s) 51
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
AseI ATTAAT 1 cut(s) 708
AspS9I GGNCC 1 cut(s) 414
AsuHPI GGTGA 1 cut(s) 463
AvaI CYCGRG 1 cut(s) 41
AvaII GGWCC 1 cut(s) 414
BaeI ACNNNNGTAYC 4 cut(s) 72, 105, 546, 579
BalI TGGCCA 2 cut(s) 66, 99
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 33, 573
BbvI GCAGC 3 cut(s) 247, 247, 784
BccI CCATC 3 cut(s) 56, 482, 692
BcoDI GTCTC 2 cut(s) 122, 619
BfaI CTAG 2 cut(s) 6, 614
BfuAI ACCTGC 1 cut(s) 481
BglII AGATCT 2 cut(s) 310, 608
BisI GCNGC 3 cut(s) 236, 261, 798
BlsI GCNGC 3 cut(s) 237, 262, 799
Bme18I GGWCC 1 cut(s) 414
BmeT110I CYCGRG 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 414
BmiI GGNNCC 2 cut(s) 415, 703
BmrI ACTGGG 2 cut(s) 69, 213
BmsI GCATC 4 cut(s) 60, 94, 247, 765
BmuI ACTGGG 2 cut(s) 69, 213
BpmI CTGGAG 2 cut(s) 153, 625
Bpu10I CCTNAGC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 846
Bsa29I ATCGAT 1 cut(s) 822
BsaI GGTCTC 1 cut(s) 619
Bse1I ACTGG 5 cut(s) 75, 136, 208, 358, 403
BseCI ATCGAT 1 cut(s) 822
BseGI GGATG 1 cut(s) 756
BseMII CTCAG 3 cut(s) 222, 807, 824
BseNI ACTGG 5 cut(s) 75, 136, 208, 358, 403
BseXI GCAGC 3 cut(s) 247, 247, 784
BseYI CCCAGC 1 cut(s) 24
BshFI GGCC 3 cut(s) 66, 99, 341
BshVI ATCGAT 1 cut(s) 822
BsiHKAI GWGCWC 2 cut(s) 33, 573
BsiHKCI CYCGRG 1 cut(s) 41
BslFI GGGAC 2 cut(s) 134, 220
BsmAI GTCTC 2 cut(s) 122, 619
BsmFI GGGAC 2 cut(s) 134, 220
BsnI GGCC 3 cut(s) 66, 99, 341
Bso31I GGTCTC 1 cut(s) 619
BsoBI CYCGRG 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 33, 573
Bsp143I GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
BspACI CCGC 3 cut(s) 214, 488, 754
BspANI GGCC 3 cut(s) 66, 99, 341
BspCNI CTCAG 3 cut(s) 223, 806, 825
BspDI ATCGAT 1 cut(s) 822
BspLI GGNNCC 2 cut(s) 415, 703
BspMI ACCTGC 1 cut(s) 481
BspTNI GGTCTC 1 cut(s) 619
BsrI ACTGG 5 cut(s) 75, 136, 208, 358, 403
BssMI GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
Bst4CI ACNGT 3 cut(s) 88, 184, 292
Bst6I CTCTTC 1 cut(s) 227
BstAPI GCANNNNNTGC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 139, 143
BstDEI CTNAG 4 cut(s) 231, 424, 793, 833
BstF5I GGATG 1 cut(s) 756
BstKTI GATC 7 cut(s) 172, 313, 325, 611, 822, 826, 839
BstMAI GTCTC 2 cut(s) 122, 619
BstMBI GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
BstMWI GCNNNNNNNGC 2 cut(s) 21, 803
BstNSI RCATGY 1 cut(s) 149
BstV1I GCAGC 3 cut(s) 247, 247, 784
BstX2I RGATCY 2 cut(s) 310, 608
BstYI RGATCY 2 cut(s) 310, 608
Bsu15I ATCGAT 1 cut(s) 822
BsuRI GGCC 3 cut(s) 66, 99, 341
BsuTUI ATCGAT 1 cut(s) 822
BtsCI GGATG 1 cut(s) 756
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 4 cut(s) 82, 93, 100, 351
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 139, 143
Cfr13I GGNCC 1 cut(s) 414
ClaI ATCGAT 1 cut(s) 822
Csp6I GTAC 1 cut(s) 396
CviAII CATG 7 cut(s) 21, 47, 146, 539, 766, 812, 867
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 4 cut(s) 231, 424, 793, 833
DpnI GATC 7 cut(s) 171, 312, 324, 610, 821, 825, 838
DpnII GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
EaeI YGGCCR 3 cut(s) 64, 97, 339
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Ecl136II GAGCTC 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 619
Eco32I GATATC 1 cut(s) 657
Eco47I GGWCC 1 cut(s) 414
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 106
Eco88I CYCGRG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 51
EcoRV GATATC 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 33
FaeI CATG 7 cut(s) 24, 50, 149, 542, 769, 815, 870
FaqI GGGAC 2 cut(s) 134, 220
FatI CATG 7 cut(s) 20, 46, 145, 538, 765, 811, 866
FauI CCCGC 1 cut(s) 761
FauNDI CATATG 1 cut(s) 778
Fnu4HI GCNGC 3 cut(s) 236, 261, 798
FokI GGATG 1 cut(s) 743
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 236, 261, 798
FspBI CTAG 2 cut(s) 6, 614
GluI GCNGC 3 cut(s) 236, 261, 798
GsaI CCCAGC 1 cut(s) 28
GsuI CTGGAG 2 cut(s) 153, 625
HaeIII GGCC 3 cut(s) 66, 99, 341
Hin1II CATG 7 cut(s) 24, 50, 149, 542, 769, 815, 870
HindIII AAGCTT 1 cut(s) 461
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 2 cut(s) 359, 471
Hpy188III TCNNGA 6 cut(s) 173, 246, 320, 614, 622, 730
Hpy8I GTNNAC 2 cut(s) 359, 471
HpyAV CCTTC 2 cut(s) 127, 492
HpyCH4III ACNGT 3 cut(s) 88, 184, 292
HpyCH4V TGCA 3 cut(s) 351, 800, 847
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 803
HpyF3I CTNAG 4 cut(s) 231, 424, 793, 833
Hsp92II CATG 7 cut(s) 24, 50, 149, 542, 769, 815, 870
Kzo9I GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
LmnI GCTCC 3 cut(s) 28, 134, 701
Lsp1109I GCAGC 3 cut(s) 247, 247, 784
LweI GCATC 4 cut(s) 60, 94, 247, 765
MaeI CTAG 2 cut(s) 6, 614
MaeIII GTNAC 1 cut(s) 718
MalI GATC 7 cut(s) 171, 312, 324, 610, 821, 825, 838
MboI GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
MboII GAAGA 3 cut(s) 112, 214, 266
MflI RGATCY 2 cut(s) 310, 608
MhlI GDGCHC 2 cut(s) 33, 573
MlsI TGGCCA 2 cut(s) 66, 99
MluCI AATT 7 cut(s) 16, 51, 268, 303, 335, 346, 640
MluNI TGGCCA 2 cut(s) 66, 99
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 7 cut(s) 50, 272, 554, 682, 749, 784, 870
Mox20I TGGCCA 2 cut(s) 66, 99
MscI TGGCCA 2 cut(s) 66, 99
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 2 cut(s) 66, 99
MspA1I CMGCKG 1 cut(s) 797
MwoI GCNNNNNNNGC 2 cut(s) 21, 803
NdeI CATATG 1 cut(s) 778
NdeII GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
NlaIII CATG 7 cut(s) 24, 50, 149, 542, 769, 815, 870
NlaIV GGNNCC 2 cut(s) 415, 703
NspI RCATGY 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 41
PkrI GCNGC 3 cut(s) 237, 262, 799
PpuMI RGGWCCY 1 cut(s) 414
PshBI ATTAAT 1 cut(s) 708
PsiI TTATAA 1 cut(s) 519
Psp124BI GAGCTC 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 414
PspFI CCCAGC 1 cut(s) 24
PspN4I GGNNCC 2 cut(s) 415, 703
PspPI GGNCC 1 cut(s) 414
PspPPI RGGWCCY 1 cut(s) 414
PspXI VCTCGAGB 1 cut(s) 41
PsuI RGATCY 2 cut(s) 310, 608
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 558
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 3 cut(s) 236, 261, 798
Sau3AI GATC 7 cut(s) 169, 310, 322, 608, 819, 823, 836
Sau96I GGNCC 1 cut(s) 414
SduI GDGCHC 2 cut(s) 33, 573
SetI ASST 9 cut(s) 33, 61, 119, 240, 465, 495, 589, 741, 799
SfaNI GCATC 4 cut(s) 60, 94, 247, 765
Sfr274I CTCGAG 1 cut(s) 41
SinI GGWCC 1 cut(s) 414
SlaI CTCGAG 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 2 cut(s) 41, 861
SmoI CTYRAG 2 cut(s) 41, 861
Sse9I AATT 7 cut(s) 16, 51, 268, 303, 335, 346, 640
SsiI CCGC 3 cut(s) 214, 488, 754
SspMI CTAG 2 cut(s) 6, 614
SstI GAGCTC 1 cut(s) 33
TaaI ACNGT 3 cut(s) 88, 184, 292
TaqI TCGA 5 cut(s) 42, 174, 325, 621, 822
TasI AATT 7 cut(s) 16, 51, 268, 303, 335, 346, 640
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 4 cut(s) 82, 93, 100, 358
TseI GCWGC 3 cut(s) 235, 260, 797
TspDTI ATGAA 4 cut(s) 9, 207, 597, 754
TspRI CASTG 4 cut(s) 82, 93, 100, 358
VpaK11BI GGWCC 1 cut(s) 414
VspI ATTAAT 1 cut(s) 708
XapI RAATTY 1 cut(s) 51
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 41
XspI CTAG 2 cut(s) 6, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.