AT5G20200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011048
Physical Location & Seq
Forward (+)
6816612 .. 6821827
5216 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20200.1

Sequence Viewer

Length: 2289 bp
ATGGCCACTCAAGGAGAAGCGACGTTCTCCGCGGCCGCGACGACGACGTCGAGCTATCCGACGGGAGGTGTTGGTGGCAAATTGAAGCGGCAATCGGCGAGGAGGCATGCGGCGACACCTTATTCTCGACCAACACAGAACCAGGTACAACGGAGGCCGTGGATCTCGAGAATCGTCGATCCTGCTTATCGGATTATTTCCGGCGGCGCTACCAGAATACTTCCATATTTCTTCTCGAACGCCGCCTCCGCTCCTGCTCTTGCTGCTCCTCCAGAGGATCAAAATCAACATCAAGGTGAGTTGCAGAATAATCCACAGGACAATGATCCCAGTGTAACCCCAATTTCAAATAAACCGGAGCCAGCATCAATTGAAGTGGGAGGTCCTAGTGGTACTGCAAACGTGAACGAAGGCAATTTCAGTATCAGTGCACAGAGAAGAGGGAAAGCAGCTTTAAATGACGACGTTGCAATCTCGGAGCTTGAAAGGCTAATGGAAGGAAAAACGTTTTCACAAGCTGAAATTGATCGTCTCATAGAGATGATAAGTTCGAGGGCTATTGATCTACCTGATGTCAAGAGAGACGAGAGAAATTTGGAGATTCCTTTGAGAGAGGGAGCCAAGAAAAATATGAGTTTATTTGATAAGGCAAAAGAGCCCATTGGTGGCAAGGATGCTAATAGTGAAATATGGGCTACACCAACCCCACTTGCAAAGTCAATAATACTTGACGGAGACAAAATACGAGATGAAGTTGGTCTTTCACCGGCAGAACTTGCCAAGGCATATATGGGAGGTCAGACATCATCAAGTAGTTCCCAAGGTTTTGTAGCAAGAAATGAAAAGGATTGTTTAGATAGGAGTATGCTTGTAGGGAAATCATCACTTGCATCACCATCAAGCAAGCCTTCTGCTTGTTGGCCTGGTATCAAGTCAAGTGAGCAATCTGGTTTTGCAACTCCTCAAAGCCGACGAGAGAGCTATGGGCTTCAAAATTTTCCAAGGACTCCTTATTCTAGAACCATTCTTTCAAATTCTAAGTCAAAGTTGATGCAATTGCAGAACGATAGTAGCAAGCACCTAAGCAACCTCCAGTCTCCCTCTCAAAGTGTGGAGAGAAGATATGGCCAGCTTAGCAAGGGAAGGGATGGTGGACTTTTTGGACCCAGTAGAAGAACTCGTCAGAGCGCCACGCCGTCCATGGTGTCTCCTTATTCACGACCTTCCCGTGGTGCATCTCGTTTTGAAAATTCTGCTATCATGAAGAGCTCTGAAGCAGGGGAATCAAGTTACCTATCTAGGTCCCAGATAACAACTTATGGTAAGCATAAAGAGGCAGAAGTTGGTACCCTGACTGTGCCTACACATTCTAGTCAGATTGCTAGGACAATATTGGATCACCTTGAACGGACCCAATCCCAATCCACCCCAAAAAATAAAACTGCCGAGTTAAAGCTTGCCACTTCCTGGAGACATCCACAGTCTTCGAAAACCGTTGAAAAAAGCAGCTCGGACGTCACCAATGTGAAAAAGGATGGCTCAGCTAAATTGCATGAAGATATCCAGAACATTTTCTCTCAGAATCAGCCATCCTCTGTTCTTAAACCACCTGCAACTACCACTGGTGACATTCAAAATGGAATGAACAAGACCGCTTCAGCGACAAATGGAATATTCCGTGGAACACAAGCAGCAAGTAGCGGTGGTAACGCACTCCAATATGAGTTTGGGAAGCCTAAGGGTTCTCTTTCCAGAAGCATGCATGATGAGTTGGGTACTTCTTCTCAGGACGCTGCAAAAGCTGTTCCCTATTCTTTTGGAGGGGAAACTGCAAATCTCCCCAAACCACCATCTCACTCACTGGGAAATAACAAACCGGTACTTCCATCGATCTCAGTAGCCAAGCCCTTCCAAAAATGGGCAGTCCCTTCGGGTTCAAACGCTGGCTTCACATTCCCGGTCTCTTCATCTGATGGAACAACATCATCAGAGCCCACAACTCCATCCATCATGCCCTTCACAACAAGCCCACCAGTAGCAAGTGGTGGTGGTGTTGCCATTACGAATCATCACGAAGCGAGAAAAGATTACGAGATTCCTCAATTTAGTTTCGACGGCAGCAACAGAAGAGGAGATAAGTCGCCCCTGGTGTTTTCGTTTCCCTCTGTAAGCGAAGAGGTGGTCAGTGAGGATGATGATGCGAGGTTTGGTATCAAATACACATTTGGGTCAGAAAAGCCGGAGAGGATATCATTCAGCTCAGCAGGAAGTGACGGTGTCTGCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

762

Amino Acids

81.68

Weight (kDa)

9.31

Isoelectric Point (pI)

59.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20200
fragaria_vesca FvH4_1g03050 FvH4_1g03050
malus_domestica MD02G1029800.v1.1 MD15G1171200.v1.1
prunus_persica Prupe.7G245100_v2.0.a1 Prupe.7G245100_v2.0.a1
pyrus_communis pycom02g02480 pycom15g15280
rosa_chinensis RchiOBHm_Chr2g0088181
rosa_laevigata RLG00000015929
rosa_multiflora Rmu_sc0003275.1_g000068
rosa_roxburghii Rroxscaffold_2G00152850
rosa_rugosa Rorug01G0481400 Rorug01G0481500
rosa_samantha Rh2AG036600 Rh2BG035800 Rh2CG036900 Rh2DG036300
rosa_wichuraiana Rw2G002880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1618
AasI GACNNNNNNGTC 1 cut(s) 46
AatII GACGTC 2 cut(s) 50, 1518
Acc36I ACCTGC 1 cut(s) 1618
Acc65I GGTACC 1 cut(s) 1346
AccB1I GGYRCC 1 cut(s) 1346
AccB7I CCANNNNNTGG 1 cut(s) 1467
AccBSI CCGCTC 1 cut(s) 251
AccII CGCG 2 cut(s) 32, 38
AclI AACGTT 1 cut(s) 506
AclWI GGATC 5 cut(s) 170, 173, 285, 320, 1404
AcoI YGGCCR 3 cut(s) 3, 33, 1126
AcsI RAATTY 4 cut(s) 592, 994, 1033, 1249
AcuI CTGAAG 2 cut(s) 1293, 1641
AcyI GRCGYC 2 cut(s) 47, 1515
AfaI GTAC 5 cut(s) 147, 394, 1348, 1777, 1881
AfiI CCNNNNNNNGG 5 cut(s) 65, 665, 1229, 1467, 1918
AgeI ACCGGT 1 cut(s) 1876
AjnI CCWGG 4 cut(s) 141, 922, 1466, 2145
AleI CACNNNNGTG 1 cut(s) 1523
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
Alw21I GWGCWC 2 cut(s) 433, 1271
Alw26I GTCTC 7 cut(s) 536, 576, 729, 1101, 1212, 1465, 1966
Alw44I GTGCAC 1 cut(s) 429
AlwI GGATC 5 cut(s) 170, 173, 285, 320, 1404
Ama87I CYCGRG 1 cut(s) 166
AoxI GGCC 5 cut(s) 3, 33, 155, 920, 1126
ApaLI GTGCAC 1 cut(s) 429
ApeKI GCWGC 6 cut(s) 263, 449, 1506, 1691, 1793, 2118
ApoI RAATTY 4 cut(s) 592, 994, 1033, 1249
ArsI GACNNNNNNTTYG 4 cut(s) 1025, 1057, 2166, 2198
AsiGI ACCGGT 1 cut(s) 1876
Asp700I GAANNNNTTC 1 cut(s) 1571
Asp718I GGTACC 1 cut(s) 1346
AspLEI GCGC 2 cut(s) 209, 1190
AspS9I GGNCC 4 cut(s) 383, 1163, 1302, 1410
AsuC2I CCSGG 1 cut(s) 1958
AsuHPI GGTGA 6 cut(s) 308, 756, 885, 1391, 1510, 1637
AsuII TTCGAA 1 cut(s) 1487
AvaI CYCGRG 1 cut(s) 166
AvaII GGWCC 4 cut(s) 383, 1163, 1302, 1410
AxyI CCTNAGG 1 cut(s) 1737
BaeGI GKGCMC 1 cut(s) 433
BalI TGGCCA 2 cut(s) 5, 1128
BanI GGYRCC 1 cut(s) 1346
BanII GRGCYC 3 cut(s) 660, 1271, 1995
BbsI GAAGAC 1 cut(s) 1476
Bbv12I GWGCWC 2 cut(s) 433, 1271
BbvI GCAGC 6 cut(s) 250, 461, 1518, 1703, 1780, 2130
BccI CCATC 9 cut(s) 904, 1142, 1529, 1597, 1858, 1894, 1967, 2011, 2015
BceAI ACGGC 3 cut(s) 142, 1180, 2131
BcgI CGANNNNNNTGC 2 cut(s) 1911, 1945
BciT130I CCWGG 4 cut(s) 143, 924, 1468, 2147
BcnI CCSGG 1 cut(s) 1958
BcoDI GTCTC 7 cut(s) 536, 576, 729, 1101, 1212, 1465, 1966
BfaI CTAG 5 cut(s) 387, 1017, 1299, 1371, 1383
BfoI RGCGCY 2 cut(s) 210, 1191
BfuAI ACCTGC 1 cut(s) 1618
BlpI GCTNAGC 3 cut(s) 1133, 1540, 2260
Bme1390I CCNGG 5 cut(s) 143, 924, 1468, 1958, 2147
Bme18I GGWCC 4 cut(s) 383, 1163, 1302, 1410
BmeT110I CYCGRG 1 cut(s) 166
BmgT120I GGNCC 4 cut(s) 383, 1163, 1302, 1410
BmiI GGNNCC 6 cut(s) 360, 619, 1165, 1304, 1348, 1412
BmrFI CCNGG 5 cut(s) 143, 924, 1468, 1958, 2147
BmrI ACTGGG 3 cut(s) 324, 1161, 1871
BmsI GCATC 6 cut(s) 374, 664, 899, 1041, 1244, 2188
BmuI ACTGGG 3 cut(s) 324, 1161, 1871
BpiI GAAGAC 1 cut(s) 1476
BpmI CTGGAG 3 cut(s) 255, 1076, 1489
Bpu10I CCTNAGC 1 cut(s) 1082
Bpu1102I GCTNAGC 3 cut(s) 1133, 1540, 2260
Bpu14I TTCGAA 1 cut(s) 1487
BpuMI CCSGG 1 cut(s) 1958
Bsa29I ATCGAT 1 cut(s) 1889
BsaBI GATNNNNATC 1 cut(s) 282
BsaHI GRCGYC 2 cut(s) 47, 1515
BsaI GGTCTC 1 cut(s) 1966
BsaJI CCNNGG 9 cut(s) 30, 158, 780, 820, 1001, 1200, 1228, 1678, 2145
BsaWI WCCGGW 2 cut(s) 355, 1876
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 5 cut(s) 65, 665, 1229, 1467, 1918
Bse118I RCCGGY 2 cut(s) 766, 1876
Bse1I ACTGG 6 cut(s) 330, 1093, 1167, 1627, 1866, 2033
Bse21I CCTNAGG 1 cut(s) 1737
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 4 cut(s) 143, 924, 1468, 2147
BseCI ATCGAT 1 cut(s) 1889
BseDI CCNNGG 9 cut(s) 30, 158, 780, 820, 1001, 1200, 1228, 1678, 2145
BseGI GGATG 7 cut(s) 679, 1153, 1474, 1540, 1589, 2003, 2197
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 5 cut(s) 65, 665, 1229, 1467, 1918
BseMII CTCAG 5 cut(s) 1554, 1592, 1799, 1908, 2274
BseNI ACTGG 6 cut(s) 330, 1093, 1167, 1627, 1866, 2033
BseRI GAGGAG 4 cut(s) 115, 258, 951, 2145
BseSI GKGCMC 1 cut(s) 433
BseX3I CGGCCG 1 cut(s) 33
BseXI GCAGC 6 cut(s) 250, 461, 1518, 1703, 1780, 2130
Bsh1236I CGCG 2 cut(s) 32, 38
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 5 cut(s) 5, 35, 157, 922, 1128
BshNI GGYRCC 1 cut(s) 1346
BshTI ACCGGT 1 cut(s) 1876
BshVI ATCGAT 1 cut(s) 1889
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 433, 1271
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 6 cut(s) 201, 356, 767, 1877, 1958, 2240
BslFI GGGAC 2 cut(s) 1288, 1910
BslI CCNNNNNNNGG 5 cut(s) 65, 665, 1229, 1467, 1918
BsmAI GTCTC 7 cut(s) 536, 576, 729, 1101, 1212, 1465, 1966
BsmBI CGTCTC 2 cut(s) 536, 576
BsmFI GGGAC 2 cut(s) 1288, 1910
BsnI GGCC 5 cut(s) 5, 35, 157, 922, 1128
Bso31I GGTCTC 1 cut(s) 1966
BsoBI CYCGRG 1 cut(s) 166
Bsp119I TTCGAA 1 cut(s) 1487
Bsp1286I GDGCHC 4 cut(s) 433, 660, 1271, 1995
Bsp143I GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
Bsp1720I GCTNAGC 3 cut(s) 1133, 1540, 2260
Bsp19I CCATGG 1 cut(s) 1200
BspANI GGCC 5 cut(s) 5, 35, 157, 922, 1128
BspCNI CTCAG 5 cut(s) 1553, 1591, 1798, 1907, 2273
BspDI ATCGAT 1 cut(s) 1889
BspFNI CGCG 2 cut(s) 32, 38
BspHI TCATGA 1 cut(s) 1260
BspLI GGNNCC 6 cut(s) 360, 619, 1165, 1304, 1348, 1412
BspMI ACCTGC 1 cut(s) 1618
BspPI GGATC 5 cut(s) 170, 173, 285, 320, 1404
BspQI GCTCTTC 1 cut(s) 1259
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 1 cut(s) 1346
BspTNI GGTCTC 1 cut(s) 1966
BsrBI CCGCTC 1 cut(s) 251
BsrFI RCCGGY 2 cut(s) 766, 1876
BsrI ACTGG 6 cut(s) 330, 1093, 1167, 1627, 1866, 2033
BssAI RCCGGY 2 cut(s) 766, 1876
BssECI CCNNGG 9 cut(s) 30, 158, 780, 820, 1001, 1200, 1228, 1678, 2145
BssMI GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
BssNI GRCGYC 2 cut(s) 47, 1515
BssT1I CCWWGG 4 cut(s) 780, 820, 1001, 1200
Bst2UI CCWGG 4 cut(s) 143, 924, 1468, 2147
Bst4CI ACNGT 4 cut(s) 1357, 1482, 1495, 2276
Bst6I CTCTTC 5 cut(s) 433, 1259, 1969, 2122, 2169
BstACI GRCGYC 2 cut(s) 47, 1515
BstAPI GCANNNNNTGC 1 cut(s) 776
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 8 cut(s) 108, 363, 905, 1076, 1130, 1458, 1760, 1945
BstDEI CTNAG 9 cut(s) 1038, 1082, 1133, 1540, 1578, 1737, 1785, 1894, 2260
BstDSI CCRYGG 5 cut(s) 30, 158, 1200, 1228, 1678
BstF5I GGATG 7 cut(s) 679, 1153, 1474, 1540, 1589, 2003, 2197
BstFNI CGCG 2 cut(s) 32, 38
BstH2I RGCGCY 2 cut(s) 210, 1191
BstHHI GCGC 2 cut(s) 209, 1190
BstKTI GATC 8 cut(s) 165, 181, 280, 328, 529, 565, 1399, 1893
BstMAI GTCTC 7 cut(s) 536, 576, 729, 1101, 1212, 1465, 1966
BstMBI GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 6 cut(s) 248, 263, 487, 776, 1134, 1799
BstNI CCWGG 4 cut(s) 143, 924, 1468, 2147
BstNSI RCATGY 2 cut(s) 110, 1762
BstSCI CCNGG 5 cut(s) 141, 922, 1466, 1956, 2145
BstSLI GKGCMC 1 cut(s) 433
BstUI CGCG 2 cut(s) 32, 38
BstV1I GCAGC 6 cut(s) 250, 461, 1518, 1703, 1780, 2130
BstV2I GAAGAC 1 cut(s) 1476
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BstZI CGGCCG 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 1889
Bsu36I CCTNAGG 1 cut(s) 1737
BsuRI GGCC 5 cut(s) 5, 35, 157, 922, 1128
BsuTUI ATCGAT 1 cut(s) 1889
BtgI CCRYGG 5 cut(s) 30, 158, 1200, 1228, 1678
BtsCI GGATG 7 cut(s) 679, 1153, 1474, 1540, 1589, 2003, 2197
BtsIMutI CAGTG 5 cut(s) 337, 433, 1620, 1859, 2191
BveI ACCTGC 1 cut(s) 1618
Cac8I GCNNGC 8 cut(s) 108, 363, 905, 1076, 1130, 1458, 1760, 1945
CciI TCATGA 1 cut(s) 1260
CciNI GCGGCCGC 1 cut(s) 33
CfoI GCGC 2 cut(s) 209, 1190
Cfr10I RCCGGY 2 cut(s) 766, 1876
Cfr13I GGNCC 4 cut(s) 383, 1163, 1302, 1410
Cfr42I CCGCGG 1 cut(s) 33
ClaI ATCGAT 1 cut(s) 1889
CseI GACGC 1 cut(s) 1799
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 5 cut(s) 146, 393, 1347, 1776, 1880
CspAI ACCGGT 1 cut(s) 1876
CspCI CAANNNNNGTGG 2 cut(s) 357, 392
CviAII CATG 7 cut(s) 107, 1201, 1261, 1553, 1759, 1763, 2011
CviQI GTAC 5 cut(s) 146, 393, 1347, 1776, 1880
DdeI CTNAG 9 cut(s) 1038, 1082, 1133, 1540, 1578, 1737, 1785, 1894, 2260
DpnI GATC 8 cut(s) 164, 180, 279, 327, 528, 564, 1398, 1892
DpnII GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
DraI TTTAAA 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 3 cut(s) 3, 33, 1126
EagI CGGCCG 1 cut(s) 33
Eam1104I CTCTTC 5 cut(s) 433, 1259, 1969, 2122, 2169
EarI CTCTTC 5 cut(s) 433, 1259, 1969, 2122, 2169
Ecl136II GAGCTC 1 cut(s) 1269
EclXI CGGCCG 1 cut(s) 33
Eco130I CCWWGG 4 cut(s) 780, 820, 1001, 1200
Eco24I GRGCYC 3 cut(s) 660, 1271, 1995
Eco31I GGTCTC 1 cut(s) 1966
Eco32I GATATC 2 cut(s) 1561, 2250
Eco47I GGWCC 4 cut(s) 383, 1163, 1302, 1410
Eco52I CGGCCG 1 cut(s) 33
Eco53kI GAGCTC 1 cut(s) 1269
Eco57I CTGAAG 2 cut(s) 1293, 1641
Eco81I CCTNAGG 1 cut(s) 1737
Eco88I CYCGRG 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 1269
EcoO109I RGGNCCY 2 cut(s) 383, 1302
EcoRII CCWGG 4 cut(s) 141, 922, 1466, 2145
EcoRV GATATC 2 cut(s) 1561, 2250
EcoT14I CCWWGG 4 cut(s) 780, 820, 1001, 1200
EcoT22I ATGCAT 1 cut(s) 1764
EcoT38I GRGCYC 3 cut(s) 660, 1271, 1995
ErhI CCWWGG 4 cut(s) 780, 820, 1001, 1200
Esp3I CGTCTC 2 cut(s) 536, 576
FaeI CATG 7 cut(s) 110, 1204, 1264, 1556, 1762, 1766, 2014
FalI AAGNNNNNCTT 6 cut(s) 744, 776, 892, 924, 994, 1026
FaqI GGGAC 2 cut(s) 1288, 1910
FatI CATG 7 cut(s) 106, 1200, 1260, 1552, 1758, 1762, 2010
FokI GGATG 7 cut(s) 686, 1160, 1461, 1547, 1576, 1990, 2204
FriOI GRGCYC 3 cut(s) 660, 1271, 1995
FspBI CTAG 5 cut(s) 387, 1017, 1299, 1371, 1383
GlaI GCGC 2 cut(s) 208, 1189
GsuI CTGGAG 3 cut(s) 255, 1076, 1489
HaeII RGCGCY 2 cut(s) 210, 1191
HaeIII GGCC 5 cut(s) 5, 35, 157, 922, 1128
HapII CCGG 6 cut(s) 201, 356, 767, 1877, 1958, 2240
HgaI GACGC 1 cut(s) 1799
HhaI GCGC 2 cut(s) 209, 1190
Hin1I GRCGYC 2 cut(s) 47, 1515
Hin1II CATG 7 cut(s) 110, 1204, 1264, 1556, 1762, 1766, 2014
Hin6I GCGC 2 cut(s) 207, 1188
HinP1I GCGC 2 cut(s) 207, 1188
HindIII AAGCTT 1 cut(s) 1454
HinfI GANTC 7 cut(s) 171, 601, 1006, 1283, 1582, 2065, 2095
HpaII CCGG 6 cut(s) 201, 356, 767, 1877, 1958, 2240
HphI GGTGA 6 cut(s) 308, 756, 885, 1391, 1510, 1637
Hpy166II GTNNAC 3 cut(s) 406, 431, 1154
Hpy8I GTNNAC 3 cut(s) 406, 431, 1154
HpyAV CCTTC 8 cut(s) 404, 491, 918, 1137, 1233, 1918, 1938, 2026
HpyCH4III ACNGT 4 cut(s) 1357, 1482, 1495, 2276
HpyCH4IV ACGT 6 cut(s) 23, 47, 402, 465, 506, 1515
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 263, 487, 776, 1134, 1799
HpyF3I CTNAG 9 cut(s) 1038, 1082, 1133, 1540, 1578, 1737, 1785, 1894, 2260
HpySE526I ACGT 6 cut(s) 23, 47, 402, 465, 506, 1515
Hsp92I GRCGYC 2 cut(s) 47, 1515
Hsp92II CATG 7 cut(s) 110, 1204, 1264, 1556, 1762, 1766, 2014
HspAI GCGC 2 cut(s) 207, 1188
KpnI GGTACC 1 cut(s) 1350
KspI CCGCGG 1 cut(s) 33
Kzo9I GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
LguI GCTCTTC 1 cut(s) 1259
LmnI GCTCC 5 cut(s) 256, 271, 358, 478, 617
Lsp1109I GCAGC 6 cut(s) 250, 461, 1518, 1703, 1780, 2130
LweI GCATC 6 cut(s) 374, 664, 899, 1041, 1244, 2188
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 5 cut(s) 387, 1017, 1299, 1371, 1383
MaeII ACGT 6 cut(s) 23, 47, 402, 465, 506, 1515
MaeIII GTNAC 6 cut(s) 334, 1289, 1516, 1625, 1706, 2270
MalI GATC 8 cut(s) 164, 180, 279, 327, 528, 564, 1398, 1892
MbiI CCGCTC 1 cut(s) 251
MboI GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
MfeI CAATTG 2 cut(s) 369, 1055
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 4 cut(s) 433, 660, 1271, 1995
MlsI TGGCCA 2 cut(s) 5, 1128
MluNI TGGCCA 2 cut(s) 5, 1128
MlyI GAGTC 1 cut(s) 1000
MmeI TCCRAC 1 cut(s) 83
Mox20I TGGCCA 2 cut(s) 5, 1128
Mph1103I ATGCAT 1 cut(s) 1764
MroXI GAANNNNTTC 1 cut(s) 1571
MscI TGGCCA 2 cut(s) 5, 1128
MseI TTAA 4 cut(s) 455, 1451, 1602, 2287
MslI CAYNNNNRTG 3 cut(s) 294, 539, 1523
Msp20I TGGCCA 2 cut(s) 5, 1128
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 6 cut(s) 201, 356, 767, 1877, 1958, 2240
MspR9I CCNGG 5 cut(s) 143, 924, 1468, 1958, 2147
MunI CAATTG 2 cut(s) 369, 1055
MvaI CCWGG 4 cut(s) 143, 924, 1468, 2147
MvnI CGCG 2 cut(s) 32, 38
MwoI GCNNNNNNNGC 6 cut(s) 248, 263, 487, 776, 1134, 1799
NciI CCSGG 1 cut(s) 1958
NcoI CCATGG 1 cut(s) 1200
NdeII GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
NlaIII CATG 7 cut(s) 110, 1204, 1264, 1556, 1762, 1766, 2014
NlaIV GGNNCC 6 cut(s) 360, 619, 1165, 1304, 1348, 1412
NmeAIII GCCGAG 1 cut(s) 1471
NmuCI GTSAC 3 cut(s) 1516, 1625, 2270
NotI GCGGCCGC 1 cut(s) 33
NsiI ATGCAT 1 cut(s) 1764
NspI RCATGY 2 cut(s) 110, 1762
NspV TTCGAA 1 cut(s) 1487
OliI CACNNNNGTG 1 cut(s) 1523
PaeI GCATGC 2 cut(s) 110, 1762
PaeR7I CTCGAG 1 cut(s) 166
PagI TCATGA 1 cut(s) 1260
PaqCI CACCTGC 1 cut(s) 1618
PciSI GCTCTTC 1 cut(s) 1259
PcsI WCGNNNNNNNCGW 3 cut(s) 47, 56, 1225
PdmI GAANNNNTTC 1 cut(s) 1571
PfeI GAWTC 6 cut(s) 171, 601, 1283, 1582, 2065, 2095
PflFI GACNNNGTC 2 cut(s) 46, 2276
PflMI CCANNNNNTGG 1 cut(s) 1467
PfoI TCCNGGA 1 cut(s) 1466
PinAI ACCGGT 1 cut(s) 1876
PleI GAGTC 1 cut(s) 1000
PpsI GAGTC 1 cut(s) 1000
PpuMI RGGWCCY 2 cut(s) 383, 1302
Psp124BI GAGCTC 1 cut(s) 1271
Psp1406I AACGTT 1 cut(s) 506
Psp5II RGGWCCY 2 cut(s) 383, 1302
Psp6I CCWGG 4 cut(s) 141, 922, 1466, 2145
PspGI CCWGG 4 cut(s) 141, 922, 1466, 2145
PspN4I GGNNCC 6 cut(s) 360, 619, 1165, 1304, 1348, 1412
PspPI GGNCC 4 cut(s) 383, 1163, 1302, 1410
PspPPI RGGWCCY 2 cut(s) 383, 1302
PsuI RGATCY 1 cut(s) 162
PsyI GACNNNGTC 2 cut(s) 46, 2276
RsaI GTAC 5 cut(s) 147, 394, 1348, 1777, 1881
RsaNI GTAC 5 cut(s) 146, 393, 1347, 1776, 1880
RseI CAYNNNNRTG 3 cut(s) 294, 539, 1523
SacI GAGCTC 1 cut(s) 1271
SacII CCGCGG 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 1259
SaqAI TTAA 4 cut(s) 455, 1451, 1602, 2287
Sau3AI GATC 8 cut(s) 162, 178, 277, 325, 526, 562, 1396, 1890
Sau96I GGNCC 4 cut(s) 383, 1163, 1302, 1410
SchI GAGTC 1 cut(s) 1000
ScrFI CCNGG 5 cut(s) 143, 924, 1468, 1958, 2147
SduI GDGCHC 4 cut(s) 433, 660, 1271, 1995
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 6 cut(s) 374, 664, 899, 1041, 1244, 2188
Sfr274I CTCGAG 1 cut(s) 166
Sfr303I CCGCGG 1 cut(s) 33
SfuI TTCGAA 1 cut(s) 1487
SgrBI CCGCGG 1 cut(s) 33
SinI GGWCC 4 cut(s) 383, 1163, 1302, 1410
SlaI CTCGAG 1 cut(s) 166
SmiMI CAYNNNNRTG 3 cut(s) 294, 539, 1523
SmlI CTYRAG 2 cut(s) 9, 166
SmoI CTYRAG 2 cut(s) 9, 166
SphI GCATGC 2 cut(s) 110, 1762
SspI AATATT 2 cut(s) 1392, 1674
SspMI CTAG 5 cut(s) 387, 1017, 1299, 1371, 1383
SstI GAGCTC 1 cut(s) 1271
StyD4I CCNGG 5 cut(s) 141, 922, 1466, 1956, 2145
StyI CCWWGG 4 cut(s) 780, 820, 1001, 1200
TaaI ACNGT 4 cut(s) 1357, 1482, 1495, 2276
TaiI ACGT 6 cut(s) 26, 50, 405, 468, 509, 1518
TaqI TCGA 9 cut(s) 50, 127, 167, 177, 236, 551, 1487, 1889, 2112
TauI GCSGC 6 cut(s) 35, 38, 91, 113, 207, 245
TfiI GAWTC 6 cut(s) 171, 601, 1283, 1582, 2065, 2095
Tru1I TTAA 4 cut(s) 455, 1451, 1602, 2287
Tru9I TTAA 4 cut(s) 455, 1451, 1602, 2287
TscAI CASTG 5 cut(s) 337, 433, 1627, 1866, 2191
TseFI GTSAC 3 cut(s) 1516, 1625, 2270
TseI GCWGC 6 cut(s) 263, 449, 1506, 1691, 1793, 2118
Tsp45I GTSAC 3 cut(s) 1516, 1625, 2270
TspDTI ATGAA 6 cut(s) 765, 855, 1277, 1569, 1658, 1956
TspGWI ACGGA 4 cut(s) 166, 747, 1423, 1667
TspRI CASTG 5 cut(s) 337, 433, 1627, 1866, 2191
Tth111I GACNNNGTC 2 cut(s) 46, 2276
Van91I CCANNNNNTGG 1 cut(s) 1467
VneI GTGCAC 1 cut(s) 429
VpaK11BI GGWCC 4 cut(s) 383, 1163, 1302, 1410
XapI RAATTY 4 cut(s) 592, 994, 1033, 1249
XbaI TCTAGA 1 cut(s) 1016
XceI RCATGY 2 cut(s) 110, 1762
XcmI CCANNNNNNNNNTGG 3 cut(s) 787, 1198, 1724
XhoI CTCGAG 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 1571
XspI CTAG 5 cut(s) 387, 1017, 1299, 1371, 1383
ZraI GACGTC 2 cut(s) 48, 1516
Zsp2I ATGCAT 1 cut(s) 1764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.