pycom02g02480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011048
Physical Location & Seq
Reverse (-)
1536008 .. 1537824
1817 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02480.3

Sequence Viewer

Length: 1332 bp
ATGAAATGGCCACACTGCATGGCGATGAGTTCTCTTCCAAACCATTTCTTCCAACACTGTCTAAACCATCGGCATGCTGGCCTGGTTCTATGGTACAGGATCGATTATTCAACTCCACAGACTGAAAGAGGCAGATTTGGCCTTCATAATCTTCCTCGAACTCCTTATTCTAGAACTATCTTTTCAAAATCCAAATCCAAGTTAACTCAATTACAAGGTGGTAATGACAAAGCCCCAAGAATTGTATTTACTCCATTGAAACAATCGCATACTCCTATATATGGGAAGCCAAGGGGTGATACATTGGATGGTGGCTTTGGATCAGCTGGACCAATTCGCAGGTTACGACATAAATCTGCTGCGCAAACTCCTGCTACAGGATCTCCTTATGTCCGTTCCTCTTCATTTGGGCCTTCACGGGTGGAGAACTCCAGTGCTACCAAAGGCTTCTTGCCTGCCACGAAGAATTTTGAACTTGGAGGATTAAGTGGGAACTCCCAAACCCAATCATCACACAGGAAGTCTTCCAGCTTTGGTGTGCCAAGTGTTCATCCAGAGTCTAGTCATATTGCTAGGACAATCTTGGAGCACATTGATAGAAACCCGCCCACTCCTAAAGAGAAGTCAGAAGAGTTAAAGCTAGCCTTTGCATGGAAGAAAACCCCGTCTTCTGGTGTTTCTTCAGTCAACCAGAATGGACATGATAGCTTACCACTTGTAGGAGGGTTCAATTCTCGTAAGCTCATAAGTCAAGATTACCAGAAAAATGCTGACAAGGGAAATTCTCTGTTTAAGATTCCACCTGTGCAGAACACTACAAAAGCCATTGCTGTTGCAAACAATATCCCTGCTGGTGATGGGAGGGATGGAAGATCTCTGAACAAGATTACACATGAGGATTTTTCGAAGTTTGCTTCATCTGGCTCTGAGGTACTAGATCAACAAAAGAAGCCTGCATCTCAATCTTCAGGGGCTAAACGTGTGTTTCCTTCTATATCCGTTGACAAGCCCGAATCAAAATGGACATTTTCTTCTGGTAGTAGCTCTGGCTTCAGTTTCCCTGTCTCTGCATCGTCTGAAGTGTTTTCTGAACTACCAACACCATCCTTTATACCAACTTTCCTGGCAAGCAGTCAGCATCAGTCCAATGAAGATGGATACGCTGTTCCTACTTACGATTTTGGATCCAAGAAGTCAGATCCCCTTGTGTTTGCATTCCCTTCTACAAGTGCGGAAATTCAGAACGATGCTTCAGATATTAAGTTCAGTTTTGGATCAGATGAGCCGAAGTTGTCTTTCGGTTCAATAGGCAAAGACGCCATCTGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

48.13

Weight (kDa)

9.67

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20200
fragaria_vesca FvH4_1g03050 FvH4_1g03050
malus_domestica MD02G1029800.v1.1 MD15G1171200.v1.1
prunus_persica Prupe.7G245100_v2.0.a1 Prupe.7G245100_v2.0.a1
pyrus_communis pycom02g02480 pycom15g15280
rosa_chinensis RchiOBHm_Chr2g0088181
rosa_laevigata RLG00000015929
rosa_multiflora Rmu_sc0003275.1_g000068
rosa_roxburghii Rroxscaffold_2G00152850
rosa_rugosa Rorug01G0481400 Rorug01G0481500
rosa_samantha Rh2AG036600 Rh2BG035800 Rh2CG036900 Rh2DG036300
rosa_wichuraiana Rw2G002880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 330
AciI CCGC 2 cut(s) 605, 1232
AclWI GGATC 7 cut(s) 107, 328, 388, 1179, 1192, 1193, 1282
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 3 cut(s) 466, 781, 1236
AcuI CTGAAG 5 cut(s) 666, 951, 1036, 1098, 1236
AcyI GRCGYC 1 cut(s) 1317
AfaI GTAC 2 cut(s) 95, 933
AfiI CCNNNNNNNGG 5 cut(s) 281, 377, 651, 671, 719
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 6 cut(s) 111, 186, 259, 473, 730, 1305
AjnI CCWGG 2 cut(s) 81, 1122
AloI GAACNNNNNNTCC 2 cut(s) 1149, 1181
AluBI AGCT 6 cut(s) 326, 531, 640, 708, 742, 1044
AluI AGCT 6 cut(s) 326, 531, 640, 708, 742, 1044
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 1069
AlwI GGATC 7 cut(s) 107, 328, 388, 1179, 1192, 1193, 1282
AoxI GGCC 4 cut(s) 8, 79, 139, 410
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 3 cut(s) 466, 781, 1236
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 2 cut(s) 329, 410
AsuHPI GGTGA 2 cut(s) 308, 866
AsuII TTCGAA 1 cut(s) 905
AsuNHI GCTAGC 1 cut(s) 640
AvaII GGWCC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 10
BamHI GGATCC 1 cut(s) 1184
BbsI GAAGAC 2 cut(s) 516, 660
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 346
BccI CCATC 7 cut(s) 75, 302, 851, 860, 1111, 1148, 1328
BciT130I CCWGG 2 cut(s) 83, 1124
BciVI GTATCC 1 cut(s) 1151
BcoDI GTCTC 1 cut(s) 1069
BfaI CTAG 5 cut(s) 171, 561, 573, 641, 935
BfmI CTRYAG 1 cut(s) 375
BfuAI ACCTGC 1 cut(s) 330
BfuI GTATCC 1 cut(s) 1151
BglII AGATCT 1 cut(s) 872
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme1390I CCNGG 2 cut(s) 83, 1124
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 2 cut(s) 329, 410
BmiI GGNNCC 1 cut(s) 1186
BmrFI CCNGG 2 cut(s) 83, 1124
BmsI GCATC 4 cut(s) 965, 1079, 1147, 1237
BmtI GCTAGC 1 cut(s) 644
BpiI GAAGAC 2 cut(s) 516, 660
BpmI CTGGAG 1 cut(s) 415
Bpu14I TTCGAA 1 cut(s) 905
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 1317
BsaJI CCNNGG 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 367, 397
Bsc4I CCNNNNNNNGG 5 cut(s) 281, 377, 651, 671, 719
Bse1I ACTGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 825
BseBI CCWGG 2 cut(s) 83, 1124
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 4 cut(s) 313, 550, 871, 1103
BseLI CCNNNNNNNGG 5 cut(s) 281, 377, 651, 671, 719
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 1 cut(s) 918
BseNI ACTGG 1 cut(s) 432
BseXI GCAGC 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 827
BshFI GGCC 4 cut(s) 10, 81, 141, 412
BshVI ATCGAT 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 591
BslI CCNNNNNNNGG 5 cut(s) 281, 377, 651, 671, 719
BsmAI GTCTC 1 cut(s) 1069
BsmI GAATGC 1 cut(s) 1214
BsnI GGCC 4 cut(s) 10, 81, 141, 412
Bsp119I TTCGAA 1 cut(s) 905
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
BspACI CCGC 2 cut(s) 605, 1232
BspANI GGCC 4 cut(s) 10, 81, 141, 412
BspCNI CTCAG 1 cut(s) 919
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 1186
BspMI ACCTGC 1 cut(s) 330
BspOI GCTAGC 1 cut(s) 644
BspPI GGATC 7 cut(s) 107, 328, 388, 1179, 1192, 1193, 1282
BspT104I TTCGAA 1 cut(s) 905
BsrDI GCAATG 1 cut(s) 825
BsrI ACTGG 1 cut(s) 432
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
BssNI GRCGYC 1 cut(s) 1317
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 2 cut(s) 83, 1124
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 3 cut(s) 39, 406, 624
BstACI GRCGYC 1 cut(s) 1317
BstBI TTCGAA 1 cut(s) 905
BstC8I GCNNGC 6 cut(s) 75, 79, 456, 642, 954, 1129
BstDEI CTNAG 1 cut(s) 927
BstENI CCTNNNNNAGG 1 cut(s) 375
BstF5I GGATG 4 cut(s) 313, 550, 871, 1103
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 8 cut(s) 102, 323, 383, 875, 940, 1187, 1201, 1277
BstMAI GTCTC 1 cut(s) 1069
BstMBI GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstNI CCWGG 2 cut(s) 83, 1124
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 2 cut(s) 81, 1122
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 346
BstV2I GAAGAC 2 cut(s) 516, 660
BstX2I RGATCY 4 cut(s) 380, 872, 1184, 1198
BstYI RGATCY 4 cut(s) 380, 872, 1184, 1198
Bsu15I ATCGAT 1 cut(s) 102
BsuI GTATCC 1 cut(s) 1151
BsuRI GGCC 4 cut(s) 10, 81, 141, 412
BsuTUI ATCGAT 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 4 cut(s) 313, 550, 871, 1103
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 3 cut(s) 13, 55, 439
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 6 cut(s) 75, 79, 456, 642, 954, 1129
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 329, 410
ClaI ATCGAT 1 cut(s) 102
CseI GACGC 1 cut(s) 1325
Csp6I GTAC 2 cut(s) 94, 932
CviAII CATG 5 cut(s) 19, 74, 651, 701, 893
CviQI GTAC 2 cut(s) 94, 932
DdeI CTNAG 1 cut(s) 927
DpnI GATC 8 cut(s) 101, 322, 382, 874, 939, 1186, 1200, 1276
DpnII GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
EaeI YGGCCR 1 cut(s) 8
Eam1104I CTCTTC 3 cut(s) 39, 406, 624
EarI CTCTTC 3 cut(s) 39, 406, 624
Eco130I CCWWGG 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 329
Eco57I CTGAAG 5 cut(s) 666, 951, 1036, 1098, 1236
EcoNI CCTNNNNNAGG 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 81, 1122
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 5 cut(s) 22, 77, 654, 704, 896
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FatI CATG 5 cut(s) 18, 73, 650, 700, 892
FauI CCCGC 1 cut(s) 612
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 4 cut(s) 320, 537, 878, 1090
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 5 cut(s) 171, 561, 573, 641, 935
FspI TGCGCA 1 cut(s) 363
GlaI GCGC 1 cut(s) 363
GluI GCNGC 1 cut(s) 360
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 4 cut(s) 10, 81, 141, 412
HgaI GACGC 1 cut(s) 1325
HhaI GCGC 1 cut(s) 364
Hin1I GRCGYC 1 cut(s) 1317
Hin1II CATG 5 cut(s) 22, 77, 654, 704, 896
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 3 cut(s) 204, 688, 1003
HindII GTYRAC 3 cut(s) 204, 688, 1003
HinfI GANTC 3 cut(s) 557, 796, 1013
HpaI GTTAAC 1 cut(s) 204
HphI GGTGA 2 cut(s) 308, 866
Hpy166II GTNNAC 3 cut(s) 204, 688, 1003
Hpy188I TCNGA 9 cut(s) 628, 879, 928, 1078, 1090, 1198, 1242, 1255, 1279
Hpy188III TCNNGA 3 cut(s) 171, 554, 752
Hpy8I GTNNAC 3 cut(s) 204, 688, 1003
HpyAV CCTTC 4 cut(s) 152, 423, 999, 1230
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 979
HpyCH4V TGCA 7 cut(s) 18, 650, 808, 836, 956, 1070, 1214
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpyF3I CTNAG 1 cut(s) 927
HpySE526I ACGT 1 cut(s) 979
Hsp92I GRCGYC 1 cut(s) 1317
Hsp92II CATG 5 cut(s) 22, 77, 654, 704, 896
HspAI GCGC 1 cut(s) 362
KspAI GTTAAC 1 cut(s) 204
Kzo9I GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
LmnI GCTCC 1 cut(s) 586
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 4 cut(s) 965, 1079, 1147, 1237
MaeI CTAG 5 cut(s) 171, 561, 573, 641, 935
MaeII ACGT 1 cut(s) 979
MaeIII GTNAC 1 cut(s) 342
MalI GATC 8 cut(s) 101, 322, 382, 874, 939, 1186, 1200, 1276
MboI GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
MflI RGATCY 4 cut(s) 380, 872, 1184, 1198
MhlI GDGCHC 1 cut(s) 591
MlsI TGGCCA 1 cut(s) 10
MluCI AATT 7 cut(s) 209, 240, 333, 466, 730, 781, 1236
MluNI TGGCCA 1 cut(s) 10
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 8 cut(s) 122, 165, 409, 473, 716, 855, 889, 922
Mox20I TGGCCA 1 cut(s) 10
MscI TGGCCA 1 cut(s) 10
MseI TTAA 6 cut(s) 203, 485, 635, 792, 1260, 1330
MslI CAYNNNNRTG 2 cut(s) 23, 72
Msp20I TGGCCA 1 cut(s) 10
MspA1I CMGCKG 1 cut(s) 326
MspR9I CCNGG 2 cut(s) 83, 1124
Mva1269I GAATGC 1 cut(s) 1214
MvaI CCWGG 2 cut(s) 83, 1124
MwoI GCNNNNNNNGC 1 cut(s) 138
NdeII GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
NheI GCTAGC 1 cut(s) 640
NlaIII CATG 5 cut(s) 22, 77, 654, 704, 896
NlaIV GGNNCC 1 cut(s) 1186
NsbI TGCGCA 1 cut(s) 363
NspI RCATGY 1 cut(s) 77
NspV TTCGAA 1 cut(s) 905
PaeI GCATGC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 1 cut(s) 1214
PfeI GAWTC 2 cut(s) 796, 1013
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp6I CCWGG 2 cut(s) 81, 1122
PspGI CCWGG 2 cut(s) 81, 1122
PspN4I GGNNCC 1 cut(s) 1186
PspPI GGNCC 2 cut(s) 329, 410
PsuI RGATCY 4 cut(s) 380, 872, 1184, 1198
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 2 cut(s) 95, 933
RsaNI GTAC 2 cut(s) 94, 932
RseI CAYNNNNRTG 2 cut(s) 23, 72
SaqAI TTAA 6 cut(s) 203, 485, 635, 792, 1260, 1330
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 8 cut(s) 99, 320, 380, 872, 937, 1184, 1198, 1274
Sau96I GGNCC 2 cut(s) 329, 410
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 2 cut(s) 83, 1124
SduI GDGCHC 1 cut(s) 591
SfaNI GCATC 4 cut(s) 965, 1079, 1147, 1237
SfcI CTRYAG 1 cut(s) 375
SfuI TTCGAA 1 cut(s) 905
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 23, 72
SphI GCATGC 1 cut(s) 77
Sse9I AATT 7 cut(s) 209, 240, 333, 466, 730, 781, 1236
SsiI CCGC 2 cut(s) 605, 1232
SspMI CTAG 5 cut(s) 171, 561, 573, 641, 935
StyD4I CCNGG 2 cut(s) 81, 1122
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 982
TaqI TCGA 3 cut(s) 102, 157, 905
TasI AATT 7 cut(s) 209, 240, 333, 466, 730, 781, 1236
TfiI GAWTC 2 cut(s) 796, 1013
Tru1I TTAA 6 cut(s) 203, 485, 635, 792, 1260, 1330
Tru9I TTAA 6 cut(s) 203, 485, 635, 792, 1260, 1330
TscAI CASTG 3 cut(s) 20, 62, 439
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 6 cut(s) 17, 134, 393, 539, 906, 1164
TspGWI ACGGA 2 cut(s) 383, 988
TspRI CASTG 3 cut(s) 20, 62, 439
VpaK11BI GGWCC 1 cut(s) 329
XagI CCTNNNNNAGG 1 cut(s) 375
XapI RAATTY 3 cut(s) 466, 781, 1236
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 1 cut(s) 77
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 5 cut(s) 171, 561, 573, 641, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.