AT5G20580

TMEM192 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007340
Physical Location & Seq
Reverse (-)
6958597 .. 6962916
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20580.2

Sequence Viewer

Length: 1047 bp
ATGGCCAACGATCGACGCGCTTCTTCTGTCAATCCGTCGACGGAGCAAGACAATGCTATGTTTCTTGATATATTGCATGAAGCTCCTCTCTTTGGTCATCGTGAATCGAGAAGTCTTGTTGGAAGCTGCATTTATCTTATCATATTGGCATGTTATGCTATTTTAGCAGCAGGGGCACCGTGGATACTTCAGTCTGTAGACTATCTTATCCCATCGCTGCTTTGCAGCTGTAATGTTGCTTTATTGATGTTAACAGGTATGTTTCAGCAATATTTTGTCAATCAGGTCCAGAAAATACGCTTGCAGGGTTATTACAGTTTCAGTCAGAAACTGAAACATGTAGTTCGTCTGCCATTTGCAATCATGGCCTATGGGACTGCATCTATGCTACTTTTCATGGAGTGGAGACCATATGTCAGTGTTCTACCCATTTTTACTGTGCAAAGGTTCATCATGTCTGTTGAAGCAATTTCTGCTGCTTCTTTTATGATTGTATTTGTTGGTTATGTTCGCCAGTATAACTCAGTGAATTCTCAGCCCGATGTCTTAAATTCATTATATTCTCCTCTTCAACCAGCTGCTTTGGAAGGATTGAGATACCATGAAGCCGGTCGCCTTTCTGATCAACAAATGGCTCTACTGCAATATCAACGCGAGAATCTCCATTATCTCAGTGAAGAGATTCTGCGTTTGCAGGAGTCCTTAAGTAAATATGAAACGAATGGTAGTAGCACGCCTCAGGTTGATCTGGCGCATCTTGTGGCAACTCGAGATCAGGAATTACGAACACTATCTGCTGAGGTGGATCAACTCCATTCAGAGCTTAATCTCGCTCGATCTCTGATCTCTGAGAGAGATAGAGAGATACAACACGTCCGGAATACCAATAATCAGTATGTAGCAGAAAATGAAAGGTTAAGAGCTATTCTTGGAGAATGGAGTATGCGAGCTGCAAAGCTTGAACGAGCTCTGGAGGTGGAGAGAATATCAAATATTGAACTGCGGAAGAAAGTTTCAGCTCTCAGAGACCAGAGGCAGGTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM192

Protein Analysis

348

Amino Acids

39.77

Weight (kDa)

6.72

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM192 PF14802 77 - 235 7.7e-11 TMEM192 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1027
Acc36I ACCTGC 1 cut(s) 1027
AccB1I GGYRCC 1 cut(s) 175
AccI GTMKAC 2 cut(s) 38, 198
AccII CGCG 2 cut(s) 18, 654
AccIII TCCGGA 1 cut(s) 876
AciI CCGC 1 cut(s) 1003
AclWI GGATC 1 cut(s) 813
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 529, 550
AcuI CTGAAG 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 92, 1036
AflII CTTAAG 1 cut(s) 703
AflIII ACRYGT 2 cut(s) 337, 871
AgsI TTSAA 4 cut(s) 464, 572, 962, 998
AhdI GACNNNNNGTC 1 cut(s) 413
AjiI CACGTC 1 cut(s) 874
Alw21I GWGCWC 1 cut(s) 970
Alw26I GTCTC 2 cut(s) 400, 1020
AlwI GGATC 1 cut(s) 813
AlwNI CAGNNNCTG 1 cut(s) 331
Ama87I CYCGRG 1 cut(s) 768
Aor13HI TCCGGA 1 cut(s) 876
AoxI GGCC 2 cut(s) 3, 366
ApeKI GCWGC 7 cut(s) 126, 167, 217, 225, 476, 578, 950
ApoI RAATTY 2 cut(s) 529, 550
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 2 cut(s) 20, 754
AspS9I GGNCC 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 768
AvaII GGWCC 1 cut(s) 286
AxyI CCTNAGG 1 cut(s) 738
BaeGI GKGCMC 1 cut(s) 178
BaeI ACNNNNGTAYC 2 cut(s) 176, 209
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 970
Bbv12I GWGCWC 1 cut(s) 970
BbvCI CCTCAGC 1 cut(s) 798
BbvI GCAGC 7 cut(s) 113, 179, 204, 237, 463, 565, 937
BccI CCATC 1 cut(s) 220
BciVI GTATCC 1 cut(s) 177
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 400, 1020
BfmI CTRYAG 1 cut(s) 195
BfrI CTTAAG 1 cut(s) 703
BfuAI ACCTGC 1 cut(s) 1027
BfuI GTATCC 1 cut(s) 177
BisI GCNGC 7 cut(s) 127, 168, 218, 226, 477, 579, 951
BlsI GCNGC 7 cut(s) 128, 169, 219, 227, 478, 580, 952
Bme18I GGWCC 1 cut(s) 286
BmeRI GACNNNNNGTC 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 768
BmgBI CACGTC 1 cut(s) 874
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 177
BmsI GCATC 2 cut(s) 389, 763
BpmI CTGGAG 1 cut(s) 992
Bpu10I CCTNAGC 1 cut(s) 798
BsaI GGTCTC 2 cut(s) 400, 1020
BsaJI CCNNGG 1 cut(s) 179
BsaWI WCCGGW 1 cut(s) 876
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 1036
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 1 cut(s) 514
Bse21I CCTNAGG 1 cut(s) 738
BseAI TCCGGA 1 cut(s) 876
BseDI CCNNGG 1 cut(s) 179
BseLI CCNNNNNNNGG 2 cut(s) 92, 1036
BseMII CTCAG 7 cut(s) 537, 548, 685, 752, 789, 840, 1036
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 2 cut(s) 75, 555
BseSI GKGCMC 1 cut(s) 178
BseXI GCAGC 7 cut(s) 113, 179, 204, 237, 463, 565, 937
Bsh1236I CGCG 2 cut(s) 18, 654
Bsh1285I CGRYCG 2 cut(s) 13, 613
BshFI GGCC 2 cut(s) 5, 368
BshNI GGYRCC 1 cut(s) 175
BsiEI CGRYCG 2 cut(s) 13, 613
BsiHKAI GWGCWC 1 cut(s) 970
BsiHKCI CYCGRG 1 cut(s) 768
BsiSI CCGG 2 cut(s) 609, 877
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 2 cut(s) 92, 1036
BsmAI GTCTC 2 cut(s) 400, 1020
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 2 cut(s) 5, 368
Bso31I GGTCTC 2 cut(s) 400, 1020
BsoBI CYCGRG 1 cut(s) 768
Bsp1286I GDGCHC 2 cut(s) 178, 970
Bsp13I TCCGGA 1 cut(s) 876
Bsp143I GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
BspACI CCGC 1 cut(s) 1003
BspANI GGCC 2 cut(s) 5, 368
BspCNI CTCAG 7 cut(s) 536, 547, 684, 751, 790, 841, 1035
BspEI TCCGGA 1 cut(s) 876
BspFNI CGCG 2 cut(s) 18, 654
BspLI GGNNCC 1 cut(s) 177
BspMI ACCTGC 1 cut(s) 1027
BspPI GGATC 1 cut(s) 813
BspT107I GGYRCC 1 cut(s) 175
BspTI CTTAAG 1 cut(s) 703
BspTNI GGTCTC 2 cut(s) 400, 1020
BsrFI RCCGGY 1 cut(s) 608
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
Bst4CI ACNGT 3 cut(s) 180, 317, 439
Bst6I CTCTTC 2 cut(s) 573, 672
BstAFI CTTAAG 1 cut(s) 703
BstAPI GCANNNNNTGC 2 cut(s) 155, 473
BstC8I GCNNGC 3 cut(s) 302, 734, 948
BstDEI CTNAG 7 cut(s) 523, 534, 671, 738, 798, 849, 1022
BstDSI CCRYGG 1 cut(s) 179
BstFNI CGCG 2 cut(s) 18, 654
BstHHI GCGC 2 cut(s) 20, 754
BstKTI GATC 7 cut(s) 13, 625, 748, 775, 808, 839, 846
BstMAI GTCTC 2 cut(s) 400, 1020
BstMBI GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
BstMCI CGRYCG 2 cut(s) 13, 613
BstMWI GCNNNNNNNGC 5 cut(s) 155, 164, 173, 365, 473
BstNSI RCATGY 2 cut(s) 153, 341
BstSFI CTRYAG 1 cut(s) 195
BstSLI GKGCMC 1 cut(s) 178
BstUI CGCG 2 cut(s) 18, 654
BstV1I GCAGC 7 cut(s) 113, 179, 204, 237, 463, 565, 937
Bsu36I CCTNAGG 1 cut(s) 738
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 2 cut(s) 5, 368
BtgI CCRYGG 1 cut(s) 179
BtgZI GCGATG 1 cut(s) 198
BtrI CACGTC 1 cut(s) 874
BtsIMutI CAGTG 3 cut(s) 424, 531, 679
BveI ACCTGC 1 cut(s) 1027
Cac8I GCNNGC 3 cut(s) 302, 734, 948
CaiI CAGNNNCTG 1 cut(s) 331
CfoI GCGC 2 cut(s) 20, 754
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 1 cut(s) 286
CseI GACGC 1 cut(s) 24
CviAII CATG 8 cut(s) 77, 150, 338, 364, 397, 454, 602, 1044
DdeI CTNAG 7 cut(s) 523, 534, 671, 738, 798, 849, 1022
DpnI GATC 7 cut(s) 12, 624, 747, 774, 807, 838, 845
DpnII GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
DriI GACNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 573, 672
Eam1105I GACNNNNNGTC 1 cut(s) 413
EarI CTCTTC 2 cut(s) 573, 672
Ecl136II GAGCTC 1 cut(s) 968
Eco24I GRGCYC 1 cut(s) 970
Eco31I GGTCTC 2 cut(s) 400, 1020
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 968
Eco57I CTGAAG 1 cut(s) 173
Eco81I CCTNAGG 1 cut(s) 738
Eco88I CYCGRG 1 cut(s) 768
EcoICRI GAGCTC 1 cut(s) 968
EcoRI GAATTC 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 970
FaeI CATG 8 cut(s) 80, 153, 341, 367, 400, 457, 605, 1047
FaqI GGGAC 1 cut(s) 388
FatI CATG 8 cut(s) 76, 149, 337, 363, 396, 453, 601, 1043
FauNDI CATATG 1 cut(s) 412
FbaI TGATCA 1 cut(s) 622
FblI GTMKAC 2 cut(s) 38, 198
Fnu4HI GCNGC 7 cut(s) 127, 168, 218, 226, 477, 579, 951
FriOI GRGCYC 1 cut(s) 970
Fsp4HI GCNGC 7 cut(s) 127, 168, 218, 226, 477, 579, 951
GlaI GCGC 2 cut(s) 19, 753
GluI GCNGC 7 cut(s) 127, 168, 218, 226, 477, 579, 951
GsuI CTGGAG 1 cut(s) 992
HaeIII GGCC 2 cut(s) 5, 368
HapII CCGG 2 cut(s) 609, 877
HgaI GACGC 1 cut(s) 24
HhaI GCGC 2 cut(s) 20, 754
Hin1II CATG 8 cut(s) 80, 153, 341, 367, 400, 457, 605, 1047
Hin6I GCGC 2 cut(s) 18, 752
HinP1I GCGC 2 cut(s) 18, 752
HincII GTYRAC 2 cut(s) 39, 252
HindII GTYRAC 2 cut(s) 39, 252
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 4 cut(s) 104, 658, 682, 698
HpaI GTTAAC 1 cut(s) 252
HpaII CCGG 2 cut(s) 609, 877
Hpy166II GTNNAC 3 cut(s) 39, 199, 252
Hpy188I TCNGA 6 cut(s) 327, 622, 820, 843, 850, 1025
Hpy188III TCNNGA 8 cut(s) 65, 101, 108, 289, 770, 776, 877, 971
Hpy8I GTNNAC 3 cut(s) 39, 199, 252
Hpy99I CGWCG 3 cut(s) 18, 40, 43
HpyAV CCTTC 1 cut(s) 581
HpyCH4III ACNGT 3 cut(s) 180, 317, 439
HpyCH4IV ACGT 1 cut(s) 873
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 164, 173, 365, 473
HpyF3I CTNAG 7 cut(s) 523, 534, 671, 738, 798, 849, 1022
HpySE526I ACGT 1 cut(s) 873
Hsp92II CATG 8 cut(s) 80, 153, 341, 367, 400, 457, 605, 1047
HspAI GCGC 2 cut(s) 18, 752
Kpn2I TCCGGA 1 cut(s) 876
Ksp22I TGATCA 1 cut(s) 622
KspAI GTTAAC 1 cut(s) 252
Kzo9I GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
LmnI GCTCC 2 cut(s) 43, 88
Lsp1109I GCAGC 7 cut(s) 113, 179, 204, 237, 463, 565, 937
LweI GCATC 2 cut(s) 389, 763
MaeII ACGT 1 cut(s) 873
MalI GATC 7 cut(s) 12, 624, 747, 774, 807, 838, 845
MboI GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
MboII GAAGA 4 cut(s) 15, 560, 689, 1018
MhlI GDGCHC 2 cut(s) 178, 970
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 468, 529, 550, 779
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 707
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 6 cut(s) 96, 576, 747, 793, 967, 1026
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 876
MroXI GAANNNNTTC 1 cut(s) 681
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 251, 548, 704, 825, 917
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 228, 578
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 2 cut(s) 609, 877
MvnI CGCG 2 cut(s) 18, 654
MwoI GCNNNNNNNGC 5 cut(s) 155, 164, 173, 365, 473
NdeI CATATG 1 cut(s) 412
NdeII GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
NlaIII CATG 8 cut(s) 80, 153, 341, 367, 400, 457, 605, 1047
NlaIV GGNNCC 1 cut(s) 177
NspI RCATGY 2 cut(s) 153, 341
PaeR7I CTCGAG 1 cut(s) 768
PaqCI CACCTGC 1 cut(s) 1027
PciI ACATGT 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 3 cut(s) 104, 658, 682
PkrI GCNGC 7 cut(s) 128, 169, 219, 227, 478, 580, 952
Ple19I CGATCG 1 cut(s) 13
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
PscI ACATGT 1 cut(s) 337
Psp124BI GAGCTC 1 cut(s) 970
PspN4I GGNNCC 1 cut(s) 177
PspPI GGNCC 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 331
PvuI CGATCG 1 cut(s) 13
PvuII CAGCTG 2 cut(s) 228, 578
SacI GAGCTC 1 cut(s) 970
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 5 cut(s) 251, 548, 704, 825, 917
SatI GCNGC 7 cut(s) 127, 168, 218, 226, 477, 579, 951
Sau3AI GATC 7 cut(s) 10, 622, 745, 772, 805, 836, 843
Sau96I GGNCC 1 cut(s) 286
SchI GAGTC 1 cut(s) 707
SduI GDGCHC 2 cut(s) 178, 970
SfaNI GCATC 2 cut(s) 389, 763
SfcI CTRYAG 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 768
SgrDI CGTCGACG 1 cut(s) 37
SinI GGWCC 1 cut(s) 286
SlaI CTCGAG 1 cut(s) 768
SmlI CTYRAG 2 cut(s) 703, 768
SmoI CTYRAG 2 cut(s) 703, 768
Sse9I AATT 4 cut(s) 468, 529, 550, 779
SsiI CCGC 1 cut(s) 1003
SspI AATATT 2 cut(s) 272, 994
SstI GAGCTC 1 cut(s) 970
TaaI ACNGT 3 cut(s) 180, 317, 439
TaiI ACGT 1 cut(s) 876
TaqI TCGA 5 cut(s) 13, 38, 107, 769, 835
TasI AATT 4 cut(s) 468, 529, 550, 779
TfiI GAWTC 3 cut(s) 104, 658, 682
Tru1I TTAA 5 cut(s) 251, 548, 704, 825, 917
Tru9I TTAA 5 cut(s) 251, 548, 704, 825, 917
TscAI CASTG 3 cut(s) 424, 531, 679
TseI GCWGC 7 cut(s) 126, 167, 217, 225, 476, 578, 950
TspDTI ATGAA 7 cut(s) 93, 385, 439, 543, 618, 729, 924
TspGWI ACGGA 2 cut(s) 24, 56
TspRI CASTG 3 cut(s) 424, 531, 679
Vha464I CTTAAG 1 cut(s) 703
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 2 cut(s) 529, 550
XceI RCATGY 2 cut(s) 153, 341
XhoI CTCGAG 1 cut(s) 768
XmiI GTMKAC 2 cut(s) 38, 198
XmnI GAANNNNTTC 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.