MD15G1217700.v1.1

TMEM192 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007340
Physical Location & Seq
Reverse (-)
17538333 .. 17541438
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1217700.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGTCAACAGAGAGAGAAAGGCACGCTTCGCTGCCGTCAACATCGCCGGAGGACAACGCACTGTTTCTGGATATCATGCACGAGGCTCCGCTGTTCGGCCACCGCAAGTCGAGAAGCCTCTTCGGAAGCGTTTTCTACTTGTTCATACTCGCCGGCTACGCTTCCGTGGCCGTTGCGGCTCCGTGGATATTCCAGGCCATAGAAGATTTGATTCCCCAATTGCTCTGCAGCTGCAACGTGCTTCTTCTGATAGTCACAGGCATTTTTCAGCAGTATCTGGTGTACCAGGTTCAGAAAATTCGATTGCAGGGTTATTATAGTTTCAGCCAGAAGTTGAAGCATATTGTTCGCCTACCTTTTGCAGCTACTTCATATGGAACTGCTGCATTGTTACTTGTCATGGTCTGGAAACCTCACATCAGTTTCCTTACTCTCCCAGCAATATTGAGGAGTATTATGGTAATTGAAGCAATATGTGCCGCATCCTTTATGAGTGTCTATATTGGTTATGTTCACCAGTATAACTCATTAAACTCCCAGCCTGATGTCTTAAAGTCATTATATTCTCCTCTTCAACCCTCGAGTTCTTTAGAAGGTTTGAGGTATCATGATGCTGGTCGACTTTCTGATCAGCAAATGGCTCTGTTGCAATATCAGCGTGAAAACCTTCATTTTTTGAGTGAAGAGATCCTTCGGTTACAAGAATGCTTAAGTAAATACGAAGGGACTAATGATGGGAGCACACCTCAGGTTGACCTTGCCCATCTGTTGGCAGCTCGTGATCAGGAATTACGGACGCTTTCCGCTGAGATGAATCAGTTGCAGTCTGAGCTCAGGCTGGCTCGGTCTTTGATAGCTGAGAGGGATTCCGAAATCCAGCAAGTCCGGACCACCAACAATCAGTACGTAGAGGAAAACGAAAGATTGAGAGCCATTTTAGGAGAATGGAGTACCCGAGCAGCAAAGGTATTATCGTCCTACCTTGACAATGTAGTTGGGGCATCCGTATTCTTCCCTGTGAGAATTTCTGCTGACAGATTTTGTAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM192

Protein Analysis

351

Amino Acids

39.63

Weight (kDa)

6.32

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM192 PF14802 79 - 233 1.1e-13 TMEM192 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 769
AccI GTMKAC 1 cut(s) 619
AccIII TCCGGA 1 cut(s) 885
AciI CCGC 5 cut(s) 89, 103, 176, 480, 804
AclWI GGATC 1 cut(s) 682
AcoI YGGCCR 2 cut(s) 97, 168
AcsI RAATTY 2 cut(s) 297, 1023
AfaI GTAC 3 cut(s) 284, 905, 952
AfiI CCNNNNNNNGG 2 cut(s) 95, 769
AflII CTTAAG 1 cut(s) 709
AgsI TTSAA 3 cut(s) 337, 467, 575
AjnI CCWGG 2 cut(s) 192, 285
AluBI AGCT 5 cut(s) 231, 365, 776, 832, 857
AluI AGCT 5 cut(s) 231, 365, 776, 832, 857
Alw21I GWGCWC 2 cut(s) 743, 834
AlwI GGATC 1 cut(s) 682
AlwNI CAGNNNCTG 1 cut(s) 277
Ama87I CYCGRG 2 cut(s) 580, 954
Aor13HI TCCGGA 1 cut(s) 885
AoxI GGCC 3 cut(s) 97, 168, 195
ApeKI GCWGC 7 cut(s) 31, 228, 231, 362, 383, 773, 959
ApoI RAATTY 2 cut(s) 297, 1023
Asp700I GAANNNNTTC 1 cut(s) 666
AspS9I GGNCC 1 cut(s) 888
AsuHPI GGTGA 1 cut(s) 506
AvaI CYCGRG 2 cut(s) 580, 954
AvaII GGWCC 1 cut(s) 888
AxyI CCTNAGG 1 cut(s) 747
BanII GRGCYC 1 cut(s) 834
BauI CACGAG 2 cut(s) 80, 777
Bbv12I GWGCWC 2 cut(s) 743, 834
BbvI GCAGC 7 cut(s) 18, 218, 240, 370, 374, 785, 971
BccI CCATC 2 cut(s) 728, 771
BceAI ACGGC 2 cut(s) 19, 155
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 2 cut(s) 194, 287
BclI TGATCA 2 cut(s) 628, 781
BfmI CTRYAG 1 cut(s) 226
BfrI CTTAAG 1 cut(s) 709
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 9 cut(s) 32, 177, 229, 232, 363, 384, 480, 774, 960
BlsI GCNGC 9 cut(s) 33, 178, 230, 233, 364, 385, 481, 775, 961
Bme1390I CCNGG 2 cut(s) 194, 287
Bme18I GGWCC 1 cut(s) 888
BmeT110I CYCGRG 2 cut(s) 580, 954
BmgT120I GGNCC 1 cut(s) 888
BmiI GGNNCC 2 cut(s) 87, 180
BmrFI CCNGG 2 cut(s) 194, 287
BmsI GCATC 3 cut(s) 491, 601, 1010
BplI GAGNNNNNCTC 2 cut(s) 730, 762
Bpu10I CCTNAGC 1 cut(s) 833
BsaAI YACGTR 1 cut(s) 907
BsaJI CCNNGG 2 cut(s) 165, 182
BsaWI WCCGGW 1 cut(s) 885
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 769
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 517
Bse21I CCTNAGG 1 cut(s) 747
BseAI TCCGGA 1 cut(s) 885
BseBI CCWGG 2 cut(s) 194, 287
BseDI CCNNGG 2 cut(s) 165, 182
BseGI GGATG 2 cut(s) 482, 1001
BseLI CCNNNNNNNGG 2 cut(s) 95, 769
BseMII CTCAG 5 cut(s) 761, 798, 819, 847, 849
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 2 cut(s) 463, 558
BseXI GCAGC 7 cut(s) 18, 218, 240, 370, 374, 785, 971
BseYI CCCAGC 2 cut(s) 436, 537
BshFI GGCC 3 cut(s) 99, 170, 197
BsiHKAI GWGCWC 2 cut(s) 743, 834
BsiHKCI CYCGRG 2 cut(s) 580, 954
BsiSI CCGG 3 cut(s) 47, 153, 886
BslFI GGGAC 1 cut(s) 739
BslI CCNNNNNNNGG 2 cut(s) 95, 769
BsmFI GGGAC 1 cut(s) 739
BsmI GAATGC 1 cut(s) 710
BsnI GGCC 3 cut(s) 99, 170, 197
BsoBI CYCGRG 2 cut(s) 580, 954
Bsp1286I GDGCHC 2 cut(s) 743, 834
Bsp13I TCCGGA 1 cut(s) 885
Bsp143I GATC 3 cut(s) 628, 687, 781
BspACI CCGC 5 cut(s) 89, 103, 176, 480, 804
BspANI GGCC 3 cut(s) 99, 170, 197
BspCNI CTCAG 5 cut(s) 760, 799, 820, 846, 850
BspEI TCCGGA 1 cut(s) 885
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 87, 180
BspMAI CTGCAG 1 cut(s) 230
BspPI GGATC 1 cut(s) 682
BspTI CTTAAG 1 cut(s) 709
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 1 cut(s) 517
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 165, 182
BssMI GATC 3 cut(s) 628, 687, 781
BssSI CACGAG 2 cut(s) 80, 777
Bst2BI CACGAG 2 cut(s) 80, 777
Bst2UI CCWGG 2 cut(s) 194, 287
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 3 cut(s) 125, 576, 678
BstAFI CTTAAG 1 cut(s) 709
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 907
BstC8I GCNNGC 3 cut(s) 24, 154, 840
BstDEI CTNAG 5 cut(s) 747, 807, 828, 833, 858
BstDSI CCRYGG 2 cut(s) 165, 182
BstF5I GGATG 2 cut(s) 482, 1001
BstKTI GATC 3 cut(s) 631, 690, 784
BstMBI GATC 3 cut(s) 628, 687, 781
BstMWI GCNNNNNNNGC 7 cut(s) 28, 158, 167, 176, 476, 655, 829
BstNI CCWGG 2 cut(s) 194, 287
BstSCI CCNGG 2 cut(s) 192, 285
BstSFI CTRYAG 1 cut(s) 226
BstSNI TACGTA 1 cut(s) 907
BstV1I GCAGC 7 cut(s) 18, 218, 240, 370, 374, 785, 971
BstX2I RGATCY 1 cut(s) 687
BstYI RGATCY 1 cut(s) 687
Bsu36I CCTNAGG 1 cut(s) 747
BsuRI GGCC 3 cut(s) 99, 170, 197
BtgI CCRYGG 2 cut(s) 165, 182
BtgZI GCGATG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 482, 1001
BtsIMutI CAGTG 1 cut(s) 59
Cac8I GCNNGC 3 cut(s) 24, 154, 840
CaiI CAGNNNCTG 1 cut(s) 277
CciI TCATGA 1 cut(s) 607
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 888
CseI GACGC 1 cut(s) 805
CsiI ACCWGGT 1 cut(s) 285
Csp6I GTAC 3 cut(s) 283, 904, 951
CviAII CATG 3 cut(s) 76, 400, 608
CviQI GTAC 3 cut(s) 283, 904, 951
DdeI CTNAG 5 cut(s) 747, 807, 828, 833, 858
DpnI GATC 3 cut(s) 630, 689, 783
DpnII GATC 3 cut(s) 628, 687, 781
EaeI YGGCCR 2 cut(s) 97, 168
Eam1104I CTCTTC 3 cut(s) 125, 576, 678
EarI CTCTTC 3 cut(s) 125, 576, 678
Ecl136II GAGCTC 1 cut(s) 832
Eco105I TACGTA 1 cut(s) 907
Eco24I GRGCYC 1 cut(s) 834
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 888
Eco53kI GAGCTC 1 cut(s) 832
Eco81I CCTNAGG 1 cut(s) 747
Eco88I CYCGRG 2 cut(s) 580, 954
EcoICRI GAGCTC 1 cut(s) 832
EcoRII CCWGG 2 cut(s) 192, 285
EcoRV GATATC 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 834
FaeI CATG 3 cut(s) 79, 403, 611
FalI AAGNNNNNCTT 4 cut(s) 10, 42, 675, 707
FaqI GGGAC 1 cut(s) 739
FatI CATG 3 cut(s) 75, 399, 607
FauNDI CATATG 1 cut(s) 373
FbaI TGATCA 2 cut(s) 628, 781
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 9 cut(s) 32, 177, 229, 232, 363, 384, 480, 774, 960
FokI GGATG 2 cut(s) 469, 988
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 9 cut(s) 32, 177, 229, 232, 363, 384, 480, 774, 960
GluI GCNGC 9 cut(s) 32, 177, 229, 232, 363, 384, 480, 774, 960
GsaI CCCAGC 2 cut(s) 440, 541
HaeIII GGCC 3 cut(s) 99, 170, 197
HapII CCGG 3 cut(s) 47, 153, 886
HgaI GACGC 1 cut(s) 805
Hin1II CATG 3 cut(s) 79, 403, 611
HincII GTYRAC 4 cut(s) 6, 39, 620, 754
HindII GTYRAC 4 cut(s) 6, 39, 620, 754
HinfI GANTC 3 cut(s) 211, 814, 866
HpaII CCGG 3 cut(s) 47, 153, 886
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 6 cut(s) 6, 39, 283, 514, 620, 754
Hpy188I TCNGA 6 cut(s) 125, 249, 294, 628, 829, 871
Hpy188III TCNNGA 7 cut(s) 68, 111, 406, 608, 779, 785, 886
Hpy8I GTNNAC 6 cut(s) 6, 39, 283, 514, 620, 754
HpyAV CCTTC 4 cut(s) 587, 677, 701, 716
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 2 cut(s) 237, 906
HpyCH4V TGCA 8 cut(s) 79, 228, 234, 307, 362, 386, 649, 823
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 158, 167, 176, 476, 655, 829
HpyF3I CTNAG 5 cut(s) 747, 807, 828, 833, 858
HpySE526I ACGT 2 cut(s) 237, 906
Hsp92II CATG 3 cut(s) 79, 403, 611
Kpn2I TCCGGA 1 cut(s) 885
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Ksp22I TGATCA 2 cut(s) 628, 781
Kzo9I GATC 3 cut(s) 628, 687, 781
LmnI GCTCC 3 cut(s) 91, 184, 738
Lsp1109I GCAGC 7 cut(s) 18, 218, 240, 370, 374, 785, 971
LweI GCATC 3 cut(s) 491, 601, 1010
MabI ACCWGGT 1 cut(s) 285
MaeII ACGT 2 cut(s) 237, 906
MaeIII GTNAC 3 cut(s) 253, 390, 696
MalI GATC 3 cut(s) 630, 689, 783
MboI GATC 3 cut(s) 628, 687, 781
MboII GAAGA 6 cut(s) 112, 215, 236, 563, 695, 1003
MfeI CAATTG 1 cut(s) 218
MflI RGATCY 1 cut(s) 687
MhlI GDGCHC 2 cut(s) 743, 834
MluCI AATT 6 cut(s) 218, 297, 462, 788, 1023, 1045
MroI TCCGGA 1 cut(s) 885
MroNI GCCGGC 1 cut(s) 152
MroXI GAANNNNTTC 1 cut(s) 666
MseI TTAA 3 cut(s) 530, 551, 710
MspA1I CMGCKG 3 cut(s) 91, 231, 806
MspCI CTTAAG 1 cut(s) 709
MspI CCGG 3 cut(s) 47, 153, 886
MspR9I CCNGG 2 cut(s) 194, 287
MunI CAATTG 1 cut(s) 218
Mva1269I GAATGC 1 cut(s) 710
MvaI CCWGG 2 cut(s) 194, 287
MwoI GCNNNNNNNGC 7 cut(s) 28, 158, 167, 176, 476, 655, 829
NaeI GCCGGC 1 cut(s) 154
NdeI CATATG 1 cut(s) 373
NdeII GATC 3 cut(s) 628, 687, 781
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 3 cut(s) 79, 403, 611
NlaIV GGNNCC 2 cut(s) 87, 180
NmuCI GTSAC 1 cut(s) 253
PaeR7I CTCGAG 1 cut(s) 580
PagI TCATGA 1 cut(s) 607
PctI GAATGC 1 cut(s) 710
PdiI GCCGGC 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 3 cut(s) 211, 814, 866
PflMI CCANNNNNTGG 1 cut(s) 769
PkrI GCNGC 9 cut(s) 33, 178, 230, 233, 364, 385, 481, 775, 961
Ppu21I YACGTR 1 cut(s) 907
Psp124BI GAGCTC 1 cut(s) 834
Psp6I CCWGG 2 cut(s) 192, 285
PspFI CCCAGC 2 cut(s) 436, 537
PspGI CCWGG 2 cut(s) 192, 285
PspN4I GGNNCC 2 cut(s) 87, 180
PspPI GGNCC 1 cut(s) 888
PspXI VCTCGAGB 1 cut(s) 580
PstI CTGCAG 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 687
PvuII CAGCTG 1 cut(s) 231
RsaI GTAC 3 cut(s) 284, 905, 952
RsaNI GTAC 3 cut(s) 283, 904, 951
SacI GAGCTC 1 cut(s) 834
SalI GTCGAC 1 cut(s) 618
SaqAI TTAA 3 cut(s) 530, 551, 710
SatI GCNGC 9 cut(s) 32, 177, 229, 232, 363, 384, 480, 774, 960
Sau3AI GATC 3 cut(s) 628, 687, 781
Sau96I GGNCC 1 cut(s) 888
ScrFI CCNGG 2 cut(s) 194, 287
SduI GDGCHC 2 cut(s) 743, 834
SexAI ACCWGGT 1 cut(s) 285
SfaNI GCATC 3 cut(s) 491, 601, 1010
SfcI CTRYAG 1 cut(s) 226
Sfr274I CTCGAG 1 cut(s) 580
SinI GGWCC 1 cut(s) 888
SlaI CTCGAG 1 cut(s) 580
SmlI CTYRAG 2 cut(s) 580, 709
SmoI CTYRAG 2 cut(s) 580, 709
SnaBI TACGTA 1 cut(s) 907
Sse9I AATT 6 cut(s) 218, 297, 462, 788, 1023, 1045
SsiI CCGC 5 cut(s) 89, 103, 176, 480, 804
SspI AATATT 1 cut(s) 444
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 2 cut(s) 192, 285
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 2 cut(s) 240, 909
TaqI TCGA 4 cut(s) 110, 301, 581, 619
TaqII GACCGA 1 cut(s) 834
TasI AATT 6 cut(s) 218, 297, 462, 788, 1023, 1045
TauI GCSGC 2 cut(s) 179, 482
TfiI GAWTC 3 cut(s) 211, 814, 866
Tru1I TTAA 3 cut(s) 530, 551, 710
Tru9I TTAA 3 cut(s) 530, 551, 710
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 7 cut(s) 31, 228, 231, 362, 383, 773, 959
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 4 cut(s) 133, 360, 659, 827
TspGWI ACGGA 4 cut(s) 154, 171, 808, 994
TspRI CASTG 1 cut(s) 66
Van91I CCANNNNNTGG 1 cut(s) 769
Vha464I CTTAAG 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 888
XapI RAATTY 2 cut(s) 297, 1023
XhoI CTCGAG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.