MD02G1091400.v1.1

TMEM192 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007340
Physical Location & Seq
Reverse (-)
7238183 .. 7241717
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1091400.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGTCAACAGAGAGAGAACGGCAAGGTTCTCTGCCGTCAACATCGCCGGAGGACAACGCACTGTTTGTGGATATCATGCACGAGGCTACGCTGTTCGGCCACCGGAAGTCGAGGAACCTCTTCGGAAGTGTTTTCTACTTAATCATACTCGCCGGTTACTCTGCCGTGGCCGTCGCGGCTCCGTGGATATTTCAGGCCATAGACGATTTGATACCCCAATTGCTCTGCAGCTGCAACGTCCTTCTTCTGGTAGTCACAGGCATTTTTCAGCAGTATCTGGTGTCCCAGGTTCAGAAAATTCGCTTACAGGGTTATTATAGTTTCAGCCAGAAGTTGAAGCATATTGTTCGCGTACCTTTTGCAGTTACTTCATATGGAACTGCTGCATTGTTACTTGTTATGGTCTGGAGACCCAACATCAGTTTCCTTACTCTCCCAGCAATATTGAGGACTATAATGGTAATTGAAGCAATATGTGCCGGATCTTTTATGAGTCTCTATATTGCTTATGTTCACCAGTACAACTCATTAAACTCCCAGCCTGATGTCTTAAAGTCGTTATATTCTCCTCTTCAACCCTCGAGTTCTTTAGAAGGTTTGAGGTATCATGATGCTGGTCGACTTTCTGATCAGCAAATGGCTTTGTTGCAATATCAGCGTGAAAACCTTCATTTTTTGAGTGAAGAGATCCTTCAGTTACAAGAGTGCTTAAGTAAATACGAAAGGACTAATGATGGGAGCACACCTCAGGTGGACCTCGCCCATCTGTTGGCAGCTCGTGATCAGGAATTACGGACGCTTTCCGCTGAGATGAATCAATTGCAGTCTGAGCTCAGGCTTGCTCGGTCTTTGATAGCTGAGAGGGATTCCGAAATCCAGCAAGTCCGGACCACCAACAATCAGTACGTGGAGGAAAACGAAAGGTTGAGGGCCATTTTAGGAGAATGGAGTACACGAGCAGCAAAGCTTGAACGAGCACTGGAGGCGGAGCGGATCTCCAATCTTGAACTCCAAAAGAAGATTCCGACGCTCAGAAGTCAATCACAATCGTCAGCTGAACCAAGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM192

Protein Analysis

357

Amino Acids

40.36

Weight (kDa)

6.41

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM192 PF14802 78 - 233 6.1e-14 TMEM192 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 769
AccBSI CCGCTC 1 cut(s) 991
AccI GTMKAC 1 cut(s) 619
AccII CGCG 2 cut(s) 176, 351
AccIII TCCGGA 1 cut(s) 885
AciI CCGC 4 cut(s) 176, 804, 986, 991
AclWI GGATC 3 cut(s) 490, 682, 1001
AcoI YGGCCR 2 cut(s) 97, 168
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 677
AfaI GTAC 4 cut(s) 354, 521, 905, 952
AfiI CCNNNNNNNGG 2 cut(s) 247, 769
AflII CTTAAG 1 cut(s) 709
AgsI TTSAA 5 cut(s) 337, 467, 575, 971, 1007
AjnI CCWGG 1 cut(s) 285
AjuI GAANNNNNNNTTGG 4 cut(s) 407, 439, 1005, 1037
AluBI AGCT 6 cut(s) 231, 776, 832, 857, 967, 1055
AluI AGCT 6 cut(s) 231, 776, 832, 857, 967, 1055
Alw21I GWGCWC 3 cut(s) 743, 834, 979
Alw26I GTCTC 2 cut(s) 403, 500
AlwI GGATC 3 cut(s) 490, 682, 1001
AlwNI CAGNNNCTG 1 cut(s) 277
Ama87I CYCGRG 1 cut(s) 580
Aor13HI TCCGGA 1 cut(s) 885
AoxI GGCC 4 cut(s) 97, 168, 195, 930
ApeKI GCWGC 5 cut(s) 228, 231, 383, 773, 959
ApoI RAATTY 1 cut(s) 297
Asp700I GAANNNNTTC 2 cut(s) 119, 666
AspS9I GGNCC 3 cut(s) 754, 888, 930
AsuHPI GGTGA 1 cut(s) 506
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 2 cut(s) 754, 888
AxyI CCTNAGG 1 cut(s) 747
BanII GRGCYC 1 cut(s) 834
BauI CACGAG 3 cut(s) 80, 777, 954
Bbv12I GWGCWC 3 cut(s) 743, 834, 979
BbvI GCAGC 5 cut(s) 218, 240, 370, 785, 971
BccI CCATC 2 cut(s) 728, 771
BceAI ACGGC 4 cut(s) 19, 35, 149, 155
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 1 cut(s) 287
BclI TGATCA 2 cut(s) 628, 781
BcoDI GTCTC 2 cut(s) 403, 500
BfmI CTRYAG 1 cut(s) 226
BfrI CTTAAG 1 cut(s) 709
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 6 cut(s) 177, 229, 232, 384, 774, 960
BlsI GCNGC 6 cut(s) 178, 230, 233, 385, 775, 961
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 2 cut(s) 754, 888
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 3 cut(s) 754, 888, 930
BmiI GGNNCC 2 cut(s) 116, 180
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 601
BplI GAGNNNNNCTC 4 cut(s) 730, 762, 980, 1012
BpmI CTGGAG 2 cut(s) 427, 1001
Bpu10I CCTNAGC 1 cut(s) 833
BsaAI YACGTR 1 cut(s) 907
BsaI GGTCTC 1 cut(s) 403
BsaJI CCNNGG 3 cut(s) 165, 182, 285
BsaWI WCCGGW 2 cut(s) 102, 885
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 769
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 517, 984
Bse21I CCTNAGG 1 cut(s) 747
BseAI TCCGGA 1 cut(s) 885
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 3 cut(s) 165, 182, 285
BseLI CCNNNNNNNGG 2 cut(s) 247, 769
BseMII CTCAG 6 cut(s) 761, 798, 819, 847, 849, 1045
BseNI ACTGG 2 cut(s) 517, 984
BseRI GAGGAG 1 cut(s) 558
BseXI GCAGC 5 cut(s) 218, 240, 370, 785, 971
BseYI CCCAGC 2 cut(s) 436, 537
Bsh1236I CGCG 2 cut(s) 176, 351
BshFI GGCC 4 cut(s) 99, 170, 197, 932
BsiHKAI GWGCWC 3 cut(s) 743, 834, 979
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 5 cut(s) 47, 103, 153, 480, 886
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 247, 769
BsmAI GTCTC 2 cut(s) 403, 500
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 4 cut(s) 99, 170, 197, 932
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 580
Bsp1286I GDGCHC 3 cut(s) 743, 834, 979
Bsp13I TCCGGA 1 cut(s) 885
Bsp143I GATC 5 cut(s) 482, 628, 687, 781, 993
BspACI CCGC 4 cut(s) 176, 804, 986, 991
BspANI GGCC 4 cut(s) 99, 170, 197, 932
BspCNI CTCAG 6 cut(s) 760, 799, 820, 846, 850, 1044
BspEI TCCGGA 1 cut(s) 885
BspFNI CGCG 2 cut(s) 176, 351
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 116, 180
BspMAI CTGCAG 1 cut(s) 230
BspPI GGATC 3 cut(s) 490, 682, 1001
BspTI CTTAAG 1 cut(s) 709
BspTNI GGTCTC 1 cut(s) 403
BsrBI CCGCTC 1 cut(s) 991
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 517, 984
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 3 cut(s) 165, 182, 285
BssMI GATC 5 cut(s) 482, 628, 687, 781, 993
BssSI CACGAG 3 cut(s) 80, 777, 954
Bst2BI CACGAG 3 cut(s) 80, 777, 954
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 3 cut(s) 125, 576, 678
BstAFI CTTAAG 1 cut(s) 709
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 907
BstC8I GCNNGC 1 cut(s) 840
BstDEI CTNAG 6 cut(s) 747, 807, 828, 833, 858, 1031
BstDSI CCRYGG 2 cut(s) 165, 182
BstFNI CGCG 2 cut(s) 176, 351
BstKTI GATC 5 cut(s) 485, 631, 690, 784, 996
BstMAI GTCTC 2 cut(s) 403, 500
BstMBI GATC 5 cut(s) 482, 628, 687, 781, 993
BstMWI GCNNNNNNNGC 5 cut(s) 176, 476, 655, 829, 983
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 2 cut(s) 176, 351
BstV1I GCAGC 5 cut(s) 218, 240, 370, 785, 971
BstX2I RGATCY 3 cut(s) 482, 687, 993
BstYI RGATCY 3 cut(s) 482, 687, 993
Bsu36I CCTNAGG 1 cut(s) 747
BsuRI GGCC 4 cut(s) 99, 170, 197, 932
BtgI CCRYGG 2 cut(s) 165, 182
BtgZI GCGATG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 59, 977
Cac8I GCNNGC 1 cut(s) 840
CaiI CAGNNNCTG 1 cut(s) 277
CciI TCATGA 1 cut(s) 607
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 3 cut(s) 754, 888, 930
CseI GACGC 2 cut(s) 805, 1036
Csp6I GTAC 4 cut(s) 353, 520, 904, 951
CviAII CATG 2 cut(s) 76, 608
CviQI GTAC 4 cut(s) 353, 520, 904, 951
DdeI CTNAG 6 cut(s) 747, 807, 828, 833, 858, 1031
DpnI GATC 5 cut(s) 484, 630, 689, 783, 995
DpnII GATC 5 cut(s) 482, 628, 687, 781, 993
EaeI YGGCCR 2 cut(s) 97, 168
Eam1104I CTCTTC 3 cut(s) 125, 576, 678
EarI CTCTTC 3 cut(s) 125, 576, 678
EciI GGCGGA 1 cut(s) 1001
Ecl136II GAGCTC 1 cut(s) 832
Eco24I GRGCYC 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 403
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 754, 888
Eco53kI GAGCTC 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 677
Eco81I CCTNAGG 1 cut(s) 747
Eco88I CYCGRG 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 832
EcoRII CCWGG 1 cut(s) 285
EcoRV GATATC 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 834
FaeI CATG 2 cut(s) 79, 611
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 75, 607
FauNDI CATATG 1 cut(s) 373
FbaI TGATCA 2 cut(s) 628, 781
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 6 cut(s) 177, 229, 232, 384, 774, 960
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 6 cut(s) 177, 229, 232, 384, 774, 960
GluI GCNGC 6 cut(s) 177, 229, 232, 384, 774, 960
GsaI CCCAGC 2 cut(s) 440, 541
GsuI CTGGAG 2 cut(s) 427, 1001
HaeIII GGCC 4 cut(s) 99, 170, 197, 932
HapII CCGG 5 cut(s) 47, 103, 153, 480, 886
HgaI GACGC 2 cut(s) 805, 1036
Hin1II CATG 2 cut(s) 79, 611
HincII GTYRAC 3 cut(s) 6, 39, 620
HindII GTYRAC 3 cut(s) 6, 39, 620
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 4 cut(s) 493, 814, 866, 1021
HpaII CCGG 5 cut(s) 47, 103, 153, 480, 886
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 6 cut(s) 6, 39, 514, 620, 754, 953
Hpy188I TCNGA 7 cut(s) 125, 294, 628, 829, 871, 1026, 1034
Hpy188III TCNNGA 6 cut(s) 406, 608, 779, 785, 886, 1004
Hpy8I GTNNAC 6 cut(s) 6, 39, 514, 620, 754, 953
Hpy99I CGWCG 2 cut(s) 176, 1030
HpyAV CCTTC 4 cut(s) 251, 587, 677, 701
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 2 cut(s) 237, 906
HpyCH4V TGCA 7 cut(s) 79, 228, 234, 362, 386, 649, 823
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 476, 655, 829, 983
HpyF3I CTNAG 6 cut(s) 747, 807, 828, 833, 858, 1031
HpySE526I ACGT 2 cut(s) 237, 906
Hsp92II CATG 2 cut(s) 79, 611
Kpn2I TCCGGA 1 cut(s) 885
Ksp22I TGATCA 2 cut(s) 628, 781
Kzo9I GATC 5 cut(s) 482, 628, 687, 781, 993
LmnI GCTCC 3 cut(s) 184, 738, 988
Lsp1109I GCAGC 5 cut(s) 218, 240, 370, 785, 971
LweI GCATC 1 cut(s) 601
MaeII ACGT 2 cut(s) 237, 906
MaeIII GTNAC 5 cut(s) 155, 253, 364, 390, 696
MalI GATC 5 cut(s) 484, 630, 689, 783, 995
MbiI CCGCTC 1 cut(s) 991
MboI GATC 5 cut(s) 482, 628, 687, 781, 993
MboII GAAGA 5 cut(s) 112, 236, 563, 695, 1030
MfeI CAATTG 2 cut(s) 218, 818
MflI RGATCY 3 cut(s) 482, 687, 993
MhlI GDGCHC 3 cut(s) 743, 834, 979
MluCI AATT 5 cut(s) 218, 297, 462, 788, 818
MlyI GAGTC 1 cut(s) 502
MmeI TCCRAC 1 cut(s) 1049
MroI TCCGGA 1 cut(s) 885
MroXI GAANNNNTTC 2 cut(s) 119, 666
MseI TTAA 4 cut(s) 140, 530, 551, 710
MspA1I CMGCKG 3 cut(s) 231, 806, 1055
MspCI CTTAAG 1 cut(s) 709
MspI CCGG 5 cut(s) 47, 103, 153, 480, 886
MspR9I CCNGG 1 cut(s) 287
MunI CAATTG 2 cut(s) 218, 818
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 2 cut(s) 176, 351
MwoI GCNNNNNNNGC 5 cut(s) 176, 476, 655, 829, 983
NdeI CATATG 1 cut(s) 373
NdeII GATC 5 cut(s) 482, 628, 687, 781, 993
NlaIII CATG 2 cut(s) 79, 611
NlaIV GGNNCC 2 cut(s) 116, 180
NmuCI GTSAC 1 cut(s) 253
PaeR7I CTCGAG 1 cut(s) 580
PagI TCATGA 1 cut(s) 607
PdmI GAANNNNTTC 2 cut(s) 119, 666
PfeI GAWTC 3 cut(s) 814, 866, 1021
PflMI CCANNNNNTGG 1 cut(s) 769
PkrI GCNGC 6 cut(s) 178, 230, 233, 385, 775, 961
PleI GAGTC 1 cut(s) 501
PpsI GAGTC 1 cut(s) 501
Ppu21I YACGTR 1 cut(s) 907
Psp124BI GAGCTC 1 cut(s) 834
Psp6I CCWGG 1 cut(s) 285
PspFI CCCAGC 2 cut(s) 436, 537
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 116, 180
PspPI GGNCC 3 cut(s) 754, 888, 930
PspXI VCTCGAGB 1 cut(s) 580
PstI CTGCAG 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 277
PsuI RGATCY 3 cut(s) 482, 687, 993
PvuII CAGCTG 2 cut(s) 231, 1055
RsaI GTAC 4 cut(s) 354, 521, 905, 952
RsaNI GTAC 4 cut(s) 353, 520, 904, 951
SacI GAGCTC 1 cut(s) 834
SalI GTCGAC 1 cut(s) 618
SaqAI TTAA 4 cut(s) 140, 530, 551, 710
SatI GCNGC 6 cut(s) 177, 229, 232, 384, 774, 960
Sau3AI GATC 5 cut(s) 482, 628, 687, 781, 993
Sau96I GGNCC 3 cut(s) 754, 888, 930
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 3 cut(s) 743, 834, 979
SfaNI GCATC 1 cut(s) 601
SfcI CTRYAG 1 cut(s) 226
Sfr274I CTCGAG 1 cut(s) 580
SinI GGWCC 2 cut(s) 754, 888
SlaI CTCGAG 1 cut(s) 580
SmlI CTYRAG 2 cut(s) 580, 709
SmoI CTYRAG 2 cut(s) 580, 709
Sse9I AATT 5 cut(s) 218, 297, 462, 788, 818
SsiI CCGC 4 cut(s) 176, 804, 986, 991
SspI AATATT 1 cut(s) 444
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 2 cut(s) 240, 909
TaqI TCGA 3 cut(s) 110, 581, 619
TaqII GACCGA 1 cut(s) 834
TasI AATT 5 cut(s) 218, 297, 462, 788, 818
TatI WGTACW 2 cut(s) 519, 950
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 3 cut(s) 814, 866, 1021
Tru1I TTAA 4 cut(s) 140, 530, 551, 710
Tru9I TTAA 4 cut(s) 140, 530, 551, 710
TscAI CASTG 2 cut(s) 66, 984
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 5 cut(s) 228, 231, 383, 773, 959
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 3 cut(s) 360, 659, 827
TspGWI ACGGA 2 cut(s) 171, 808
TspRI CASTG 2 cut(s) 66, 984
Van91I CCANNNNNTGG 1 cut(s) 769
Vha464I CTTAAG 1 cut(s) 709
VpaK11BI GGWCC 2 cut(s) 754, 888
XapI RAATTY 1 cut(s) 297
XhoI CTCGAG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 619
XmnI GAANNNNTTC 2 cut(s) 119, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.