AT5G25270

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007815
Physical Location & Seq
Reverse (-)
8757434 .. 8763005
5572 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25270.1

Sequence Viewer

Length: 1929 bp
ATGGGAGACAATGGCAAAGATGAAATTATGGTCGAGGCATCTCAATGCGCTGGTGCTATGGTTGAGATAAAGATAAAGACGTTGGATTCTCAAACGTACACCTTACGTGTAGATAAATGTGTCCCTGTTCCCGCATTGAAGGAGCAAGTTGCCTCCGTTACTGGTGTCGTTACGGAACAACAACGCTTGATATGTCGTGGAAAAGTTATGAAGGATGATCAACTCCTATCAGCCTATCATGTTGAAGATGGCCACACTCTGCATTTGGTAGTCAGACAACCTGTATCAGAGAGTTCAACTAGTAATGCAGCGGCTGATCCTGCTTTGAGTGCTGGTGACAGCCAGGGCAGTCAACGATCACGAGTTGTAGTAGGATCCTTCAACATTGCTGAACAAGCTGATGGAGTCTATTCAGATCTTGGACAGATAGTTTCAGCTGTTCTGGGTTCACTTGGGATCTCTAATCCAGAAGGTGGTATTGAAGGGATCGATGATATGGGTCCTCTTCATGAGAGGCTGTCTCGGAGTTCTGGTCCTGGCACTGCAAGAGATTCTTCCGGAGGGCGATCTGCCACACCAAATGCTGTGGACCAGACGTCTACTCCCTTGGCATCTTCACAACCAGCAGCGATTCCAGATTCGTTGACAACCTTATCTGAATATCTGAATCATCTCAGACAAGAGTTTGCTGCTAATGGTAGCAATGCTAATAACTTGCAAGATTCTGAAAATTCAGTGGGCAATGTGCAAGATTCTGCTTCTACTACTGGAGAGAGTCGGATTCCAAGACCATCTCATTTGGCAGAAGTGCTGCAATCAACAAGGCAATTGTTGATCGGTGAAGTAGCAGATTGCTTATCTAATCTTTCGAGGCAGCTCGTGGATCATGTAAATGTGACCGATCCGCCAACCCGAAGGTTGTGCCAGTCAAACATGCTTCAATCAGGATCCCTCTTGGAGAGTTTAGGCATTTCACTCCTGGAGCTTGGCCGTGCCACCATGATGCTTCGTTTGGGACAAACACCTGATGATGCTGTTGTTGATGCTGGACCTGCAGTGTTCATATCCCCTACAGGGCGAAATCCCTTGCCGTCACACTCCTCTCGGCTGGGCACGAGTATTGGAAGCTTACAAGCAGGAACCGCACATTCTAACCCCTTTGCGGGACAATCACTTGCCTCTGCCCCAAGAAATATTGAGATAAGAATACGTACAGGATCTTGGGTGCCCGCCAGTGGGACCAACCAGAGAGAGGAAAGTACTACACAACAAACCCCTGGGCAGACAATCCCTTCTACACCCAGCAGCACTACTGATCCTGCCCCCTCAACGAGAGGTCCATCTGAACCTCTTCGAAATCCTGTGGCCTTAGTTATACCTGTTGTTGCTCGATATCAGCAGATATCTCTTGGTGGACGTAGCTCTACTGGATTGGATGGTGTACATCAGCCTGTTACTGAATCTTCTAGGCAGCCACAGAGCGTCGGTACTCCAGGAAGAGAAGGTGATAGCAGTGCTTCTCCTGGCGGTCGGGGTTTGTCTGAATTGAGAAACAGAATCCATCAGTTTTTGAGGCCCTTATCTCGTCGAGAAAACCAAGCCGGGAGCACGGAGTCTCAGGGTGCAGCAAATCCCTCTGCCACAGCGTCCACAGAAACAAACGAAGCAGTTGCAAATGCACAAGTAGAGCCAGCAACTACGACAGATGAAGGGAATTTCATATCGAGTGTTCTTCAGCAGATCATGCCGTTTATATCTCAAAACGTAGCCTCCTCAAGCTCAGGTGAAGCTGCTACAGGCAGAGGCAGCAATAGTAGACAAGCCTCTTCGAGAGAGGCAGAGGAGGAGGAAGGTACAGAGCGAGGGAATTCAACTCGGAGACCTGAACCACCATCACCTCCAGAGTCAAAACGTCAGAGGAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001775 GO:0001822 GO:0002020 GO:0002253 GO:0002376 GO:0002429 GO:0002474 GO:0002682 GO:0002684 GO:0002757 GO:0002764 GO:0002768 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010604 GO:0010605 GO:0010941 GO:0012501 GO:0016020 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019882 GO:0019899 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030101 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0030544 GO:0031072 GO:0031323 GO:0031324 GO:0031325 GO:0031329 GO:0031330 GO:0031331 GO:0031593 GO:0031625 GO:0031647 GO:0031974 GO:0031981 GO:0031982 GO:0032268 GO:0032269 GO:0032270 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036506 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042802 GO:0042981 GO:0043021 GO:0043022 GO:0043066 GO:0043067 GO:0043069 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043230 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045321 GO:0045732 GO:0045861 GO:0045862 GO:0045995 GO:0046649 GO:0048002 GO:0048232 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051787 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0060548 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070059 GO:0070062 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0080134 GO:0080135 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1901800 GO:1903046 GO:1903050 GO:1903051 GO:1903052 GO:1903362 GO:1903363 GO:1903364 GO:1903561 GO:1904292 GO:1904294 GO:1904378 GO:1905897 GO:1905898 GO:1990381 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

642

Amino Acids

67.41

Weight (kDa)

5.3

Isoelectric Point (pI)

59.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 22 - 91 5.4e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 23 - 93 4e-19 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 1060
AccB1I GGYRCC 1 cut(s) 1225
AccB7I CCANNNNNTGG 1 cut(s) 473
AccI GTMKAC 2 cut(s) 599, 1816
AccIII TCCGGA 1 cut(s) 557
AciI CCGC 7 cut(s) 132, 311, 905, 1143, 1163, 1230, 1527
AcoI YGGCCR 2 cut(s) 250, 988
AcsI RAATTY 3 cut(s) 730, 1714, 1867
AcuI CTGAAG 1 cut(s) 1718
AcyI GRCGYC 1 cut(s) 596
AfaI GTAC 6 cut(s) 98, 1213, 1261, 1443, 1489, 1855
AfiI CCNNNNNNNGG 7 cut(s) 473, 1074, 1162, 1163, 1235, 1236, 1252
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 7 cut(s) 139, 245, 297, 382, 482, 941, 1872
AhdI GACNNNNNGTC 1 cut(s) 595
AhlI ACTAGT 1 cut(s) 299
AjnI CCWGG 6 cut(s) 342, 535, 978, 1276, 1492, 1522
AluBI AGCT 8 cut(s) 398, 437, 877, 985, 1128, 1422, 1779, 1790
AluI AGCT 8 cut(s) 398, 437, 877, 985, 1128, 1422, 1779, 1790
Alw21I GWGCWC 1 cut(s) 1610
Alw26I GTCTC 3 cut(s) 525, 1620, 1873
AlwNI CAGNNNCTG 1 cut(s) 314
Aor13HI TCCGGA 1 cut(s) 557
AoxI GGCC 4 cut(s) 250, 988, 1365, 1574
ApoI RAATTY 3 cut(s) 730, 1714, 1867
Asp700I GAANNNNTTC 1 cut(s) 1350
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 7 cut(s) 500, 533, 589, 1049, 1239, 1337, 1575
AsuC2I CCSGG 1 cut(s) 1603
AsuHPI GGTGA 5 cut(s) 347, 851, 1517, 1796, 1887
AsuII TTCGAA 1 cut(s) 1354
AvaII GGWCC 6 cut(s) 500, 533, 589, 1049, 1239, 1337
BaeGI GKGCMC 2 cut(s) 1115, 1230
BalI TGGCCA 1 cut(s) 252
BamHI GGATCC 2 cut(s) 374, 947
BanI GGYRCC 1 cut(s) 1225
BauI CACGAG 3 cut(s) 360, 878, 1114
Bbv12I GWGCWC 1 cut(s) 1610
BccI CCATC 7 cut(s) 242, 395, 799, 1348, 1430, 1569, 1900
BceAI ACGGC 3 cut(s) 975, 1075, 1732
BcgI CGANNNNNNTGC 4 cut(s) 903, 937, 1652, 1686
BciT130I CCWGG 6 cut(s) 344, 537, 980, 1278, 1494, 1524
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 1 cut(s) 1603
BcoDI GTCTC 3 cut(s) 525, 1620, 1873
BcuI ACTAGT 1 cut(s) 299
BfaI CTAG 2 cut(s) 300, 1467
BfmI CTRYAG 3 cut(s) 1053, 1071, 1794
BfuAI ACCTGC 1 cut(s) 1060
BglII AGATCT 1 cut(s) 415
BmcAI AGTACT 1 cut(s) 1261
Bme1390I CCNGG 7 cut(s) 344, 537, 980, 1278, 1494, 1524, 1603
Bme18I GGWCC 6 cut(s) 500, 533, 589, 1049, 1239, 1337
BmeRI GACNNNNNGTC 1 cut(s) 595
BmgT120I GGNCC 7 cut(s) 500, 533, 589, 1049, 1239, 1337, 1575
BmiI GGNNCC 6 cut(s) 376, 501, 949, 1141, 1227, 1240
BmrFI CCNGG 7 cut(s) 344, 537, 980, 1278, 1494, 1524, 1603
BmsI GCATC 5 cut(s) 47, 620, 993, 1021, 1033
BplI GAGNNNNNCTC 2 cut(s) 505, 537
BpmI CTGGAG 4 cut(s) 789, 1001, 1476, 1884
Bpu10I CCTNAGC 1 cut(s) 1780
Bpu14I TTCGAA 1 cut(s) 1354
BpuEI CTTGAG 1 cut(s) 1759
BpuMI CCSGG 1 cut(s) 1603
Bsa29I ATCGAT 1 cut(s) 489
BsaAI YACGTR 2 cut(s) 107, 1211
BsaHI GRCGYC 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 1873
BsaJI CCNNGG 4 cut(s) 343, 606, 1276, 1277
BsaWI WCCGGW 1 cut(s) 557
BsaXI ACNNNNNCTCC 6 cut(s) 517, 547, 586, 616, 1754, 1784
Bsc4I CCNNNNNNNGG 7 cut(s) 473, 1074, 1162, 1163, 1235, 1236, 1252
Bse1I ACTGG 5 cut(s) 166, 772, 925, 1233, 1432
Bse3DI GCAATG 3 cut(s) 384, 709, 748
BseAI TCCGGA 1 cut(s) 557
BseBI CCWGG 6 cut(s) 344, 537, 980, 1278, 1494, 1524
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 4 cut(s) 343, 606, 1276, 1277
BseGI GGATG 2 cut(s) 220, 1441
BseLI CCNNNNNNNGG 7 cut(s) 473, 1074, 1162, 1163, 1235, 1236, 1252
BseMI GCAATG 3 cut(s) 384, 709, 748
BseMII CTCAG 3 cut(s) 688, 1631, 1794
BseNI ACTGG 5 cut(s) 166, 772, 925, 1233, 1432
BseRI GAGGAG 4 cut(s) 1090, 1762, 1856, 1859
BseSI GKGCMC 2 cut(s) 1115, 1230
BseYI CCCAGC 2 cut(s) 1108, 1301
BsgI GTGCAG 1 cut(s) 1644
Bsh1285I CGRYCG 1 cut(s) 1531
BshFI GGCC 4 cut(s) 252, 990, 1367, 1576
BshNI GGYRCC 1 cut(s) 1225
BshVI ATCGAT 1 cut(s) 489
BsiEI CGRYCG 1 cut(s) 1531
BsiHKAI GWGCWC 1 cut(s) 1610
BsiSI CCGG 2 cut(s) 558, 1602
BslFI GGGAC 4 cut(s) 107, 1029, 1179, 1252
BslI CCNNNNNNNGG 7 cut(s) 473, 1074, 1162, 1163, 1235, 1236, 1252
BsmAI GTCTC 3 cut(s) 525, 1620, 1873
BsmFI GGGAC 4 cut(s) 107, 1029, 1179, 1252
BsnI GGCC 4 cut(s) 252, 990, 1367, 1576
Bso31I GGTCTC 1 cut(s) 1873
Bsp119I TTCGAA 1 cut(s) 1354
Bsp1286I GDGCHC 3 cut(s) 1115, 1230, 1610
Bsp13I TCCGGA 1 cut(s) 557
Bsp1407I TGTACA 1 cut(s) 1441
BspACI CCGC 7 cut(s) 132, 311, 905, 1143, 1163, 1230, 1527
BspANI GGCC 4 cut(s) 252, 990, 1367, 1576
BspCNI CTCAG 3 cut(s) 687, 1630, 1793
BspDI ATCGAT 1 cut(s) 489
BspEI TCCGGA 1 cut(s) 557
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 6 cut(s) 376, 501, 949, 1141, 1227, 1240
BspMAI CTGCAG 1 cut(s) 1057
BspMI ACCTGC 1 cut(s) 1060
BspT104I TTCGAA 1 cut(s) 1354
BspT107I GGYRCC 1 cut(s) 1225
BspTNI GGTCTC 1 cut(s) 1873
BsrDI GCAATG 3 cut(s) 384, 709, 748
BsrGI TGTACA 1 cut(s) 1441
BsrI ACTGG 5 cut(s) 166, 772, 925, 1233, 1432
BssECI CCNNGG 4 cut(s) 343, 606, 1276, 1277
BssNI GRCGYC 1 cut(s) 596
BssSI CACGAG 3 cut(s) 360, 878, 1114
BssT1I CCWWGG 1 cut(s) 606
Bst2BI CACGAG 3 cut(s) 360, 878, 1114
Bst2UI CCWGG 6 cut(s) 344, 537, 980, 1278, 1494, 1524
Bst6I CTCTTC 4 cut(s) 510, 1356, 1492, 1831
BstACI GRCGYC 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 1744
BstAUI TGTACA 1 cut(s) 1441
BstBAI YACGTR 2 cut(s) 107, 1211
BstBI TTCGAA 1 cut(s) 1354
BstC8I GCNNGC 2 cut(s) 1230, 1692
BstDEI CTNAG 4 cut(s) 674, 1369, 1617, 1780
BstF5I GGATG 2 cut(s) 220, 1441
BstHHI GCGC 1 cut(s) 50
BstMAI GTCTC 3 cut(s) 525, 1620, 1873
BstMCI CGRYCG 1 cut(s) 1531
BstMWI GCNNNNNNNGC 7 cut(s) 320, 329, 395, 1052, 1142, 1744, 1806
BstNI CCWGG 6 cut(s) 344, 537, 980, 1278, 1494, 1524
BstNSI RCATGY 1 cut(s) 937
BstSCI CCNGG 7 cut(s) 342, 535, 978, 1276, 1492, 1522, 1601
BstSFI CTRYAG 3 cut(s) 1053, 1071, 1794
BstSLI GKGCMC 2 cut(s) 1115, 1230
BstSNI TACGTA 1 cut(s) 1211
BstX2I RGATCY 5 cut(s) 374, 415, 456, 947, 1217
BstYI RGATCY 5 cut(s) 374, 415, 456, 947, 1217
Bsu15I ATCGAT 1 cut(s) 489
BsuRI GGCC 4 cut(s) 252, 990, 1367, 1576
BsuTUI ATCGAT 1 cut(s) 489
BtsCI GGATG 2 cut(s) 220, 1441
BtsI GCAGTG 3 cut(s) 540, 1062, 1519
BtsIMutI CAGTG 5 cut(s) 540, 741, 1062, 1240, 1519
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 2 cut(s) 1230, 1692
CaiI CAGNNNCTG 1 cut(s) 314
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 50
Cfr13I GGNCC 7 cut(s) 500, 533, 589, 1049, 1239, 1337, 1575
ClaI ATCGAT 1 cut(s) 489
CseI GACGC 2 cut(s) 1471, 1635
Csp6I GTAC 6 cut(s) 97, 1212, 1260, 1442, 1488, 1854
CspCI CAANNNNNGTGG 2 cut(s) 567, 602
CviAII CATG 6 cut(s) 239, 509, 887, 934, 1000, 1744
CviQI GTAC 6 cut(s) 97, 1212, 1260, 1442, 1488, 1854
DdeI CTNAG 4 cut(s) 674, 1369, 1617, 1780
DriI GACNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 2 cut(s) 250, 988
Eam1104I CTCTTC 4 cut(s) 510, 1356, 1492, 1831
Eam1105I GACNNNNNGTC 1 cut(s) 595
EarI CTCTTC 4 cut(s) 510, 1356, 1492, 1831
EciI GGCGGA 1 cut(s) 894
Eco105I TACGTA 1 cut(s) 1211
Eco130I CCWWGG 1 cut(s) 606
Eco31I GGTCTC 1 cut(s) 1873
Eco32I GATATC 2 cut(s) 1394, 1404
Eco47I GGWCC 6 cut(s) 500, 533, 589, 1049, 1239, 1337
Eco57I CTGAAG 1 cut(s) 1718
EcoO109I RGGNCCY 2 cut(s) 500, 1575
EcoRI GAATTC 1 cut(s) 1867
EcoRII CCWGG 6 cut(s) 342, 535, 978, 1276, 1492, 1522
EcoRV GATATC 2 cut(s) 1394, 1404
EcoT14I CCWWGG 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 6 cut(s) 242, 512, 890, 937, 1003, 1747
FalI AAGNNNNNCTT 2 cut(s) 538, 570
FaqI GGGAC 4 cut(s) 107, 1029, 1179, 1252
FatI CATG 6 cut(s) 238, 508, 886, 933, 999, 1743
FauI CCCGC 3 cut(s) 139, 1156, 1237
FbaI TGATCA 1 cut(s) 217
FblI GTMKAC 2 cut(s) 599, 1816
FokI GGATG 2 cut(s) 227, 1448
FspBI CTAG 2 cut(s) 300, 1467
GlaI GCGC 1 cut(s) 49
GsaI CCCAGC 2 cut(s) 1112, 1305
GsuI CTGGAG 4 cut(s) 789, 1001, 1476, 1884
HaeIII GGCC 4 cut(s) 252, 990, 1367, 1576
HapII CCGG 2 cut(s) 558, 1602
HgaI GACGC 2 cut(s) 1471, 1635
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 596
Hin1II CATG 6 cut(s) 242, 512, 890, 937, 1003, 1747
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 2 cut(s) 353, 645
HindII GTYRAC 2 cut(s) 353, 645
HindIII AAGCTT 1 cut(s) 1126
HpaII CCGG 2 cut(s) 558, 1602
HphI GGTGA 5 cut(s) 347, 851, 1517, 1796, 1887
Hpy188III TCNNGA 9 cut(s) 360, 467, 509, 558, 635, 945, 1589, 1830, 1901
Hpy99I CGWCG 2 cut(s) 1487, 1590
HpyCH4IV ACGT 8 cut(s) 80, 95, 106, 596, 1210, 1417, 1764, 1912
HpyF10VI GCNNNNNNNGC 7 cut(s) 320, 329, 395, 1052, 1142, 1744, 1806
HpyF3I CTNAG 4 cut(s) 674, 1369, 1617, 1780
HpySE526I ACGT 8 cut(s) 80, 95, 106, 596, 1210, 1417, 1764, 1912
Hsp92I GRCGYC 1 cut(s) 596
Hsp92II CATG 6 cut(s) 242, 512, 890, 937, 1003, 1747
HspAI GCGC 1 cut(s) 48
Kpn2I TCCGGA 1 cut(s) 557
Ksp22I TGATCA 1 cut(s) 217
LmnI GCTCC 3 cut(s) 142, 982, 1605
LweI GCATC 5 cut(s) 47, 620, 993, 1021, 1033
MaeI CTAG 2 cut(s) 300, 1467
MaeII ACGT 8 cut(s) 80, 95, 106, 596, 1210, 1417, 1764, 1912
MaeIII GTNAC 6 cut(s) 157, 169, 335, 895, 1092, 1453
MboII GAAGA 9 cut(s) 257, 497, 546, 606, 1343, 1455, 1509, 1724, 1818
MfeI CAATTG 1 cut(s) 827
MflI RGATCY 5 cut(s) 374, 415, 456, 947, 1217
MhlI GDGCHC 3 cut(s) 1115, 1230, 1610
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 6 cut(s) 24, 730, 827, 1544, 1714, 1867
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 4 cut(s) 414, 784, 1622, 1913
MmeI TCCRAC 2 cut(s) 63, 758
Mox20I TGGCCA 1 cut(s) 252
MroI TCCGGA 1 cut(s) 557
MroXI GAANNNNTTC 1 cut(s) 1350
MscI TGGCCA 1 cut(s) 252
MslI CAYNNNNRTG 3 cut(s) 43, 891, 1001
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 2 cut(s) 311, 437
MspI CCGG 2 cut(s) 558, 1602
MspR9I CCNGG 7 cut(s) 344, 537, 980, 1278, 1494, 1524, 1603
MunI CAATTG 1 cut(s) 827
MvaI CCWGG 6 cut(s) 344, 537, 980, 1278, 1494, 1524
MwoI GCNNNNNNNGC 7 cut(s) 320, 329, 395, 1052, 1142, 1744, 1806
NciI CCSGG 1 cut(s) 1603
NlaIII CATG 6 cut(s) 242, 512, 890, 937, 1003, 1747
NlaIV GGNNCC 6 cut(s) 376, 501, 949, 1141, 1227, 1240
NmeAIII GCCGAG 1 cut(s) 1084
NmuCI GTSAC 3 cut(s) 335, 895, 1092
NspI RCATGY 1 cut(s) 937
NspV TTCGAA 1 cut(s) 1354
PagI TCATGA 1 cut(s) 508
PasI CCCWGGG 1 cut(s) 1277
PdmI GAANNNNTTC 1 cut(s) 1350
PflMI CCANNNNNTGG 1 cut(s) 473
PfoI TCCNGGA 2 cut(s) 978, 1492
PleI GAGTC 4 cut(s) 413, 783, 1621, 1912
PpsI GAGTC 4 cut(s) 413, 783, 1621, 1912
Ppu21I YACGTR 2 cut(s) 107, 1211
PpuMI RGGWCCY 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 6 cut(s) 342, 535, 978, 1276, 1492, 1522
PspFI CCCAGC 2 cut(s) 1108, 1301
PspGI CCWGG 6 cut(s) 342, 535, 978, 1276, 1492, 1522
PspN4I GGNNCC 6 cut(s) 376, 501, 949, 1141, 1227, 1240
PspPI GGNCC 7 cut(s) 500, 533, 589, 1049, 1239, 1337, 1575
PspPPI RGGWCCY 1 cut(s) 500
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 5 cut(s) 374, 415, 456, 947, 1217
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 6 cut(s) 98, 1213, 1261, 1443, 1489, 1855
RsaNI GTAC 6 cut(s) 97, 1212, 1260, 1442, 1488, 1854
RseI CAYNNNNRTG 3 cut(s) 43, 891, 1001
Sau96I GGNCC 7 cut(s) 500, 533, 589, 1049, 1239, 1337, 1575
ScaI AGTACT 1 cut(s) 1261
SchI GAGTC 4 cut(s) 414, 784, 1622, 1913
ScrFI CCNGG 7 cut(s) 344, 537, 980, 1278, 1494, 1524, 1603
SduI GDGCHC 3 cut(s) 1115, 1230, 1610
SfaNI GCATC 5 cut(s) 47, 620, 993, 1021, 1033
SfcI CTRYAG 3 cut(s) 1053, 1071, 1794
SfuI TTCGAA 1 cut(s) 1354
SinI GGWCC 6 cut(s) 500, 533, 589, 1049, 1239, 1337
SmiMI CAYNNNNRTG 3 cut(s) 43, 891, 1001
SmlI CTYRAG 1 cut(s) 1774
SmoI CTYRAG 1 cut(s) 1774
SnaBI TACGTA 1 cut(s) 1211
SpeI ACTAGT 1 cut(s) 299
Sse9I AATT 6 cut(s) 24, 730, 827, 1544, 1714, 1867
SsiI CCGC 7 cut(s) 132, 311, 905, 1143, 1163, 1230, 1527
SspI AATATT 1 cut(s) 1195
SspMI CTAG 2 cut(s) 300, 1467
StyD4I CCNGG 7 cut(s) 342, 535, 978, 1276, 1492, 1522, 1601
StyI CCWWGG 1 cut(s) 606
TaiI ACGT 8 cut(s) 83, 98, 109, 599, 1213, 1420, 1767, 1915
TaqI TCGA 8 cut(s) 33, 489, 869, 1354, 1390, 1588, 1724, 1829
TaqII GACCGA 1 cut(s) 914
TasI AATT 6 cut(s) 24, 730, 827, 1544, 1714, 1867
TatI WGTACW 2 cut(s) 1259, 1441
TauI GCSGC 1 cut(s) 314
TscAI CASTG 5 cut(s) 547, 741, 1062, 1240, 1519
TseFI GTSAC 3 cut(s) 335, 895, 1092
Tsp45I GTSAC 3 cut(s) 335, 895, 1092
TspDTI ATGAA 6 cut(s) 36, 224, 497, 1051, 1708, 1722
TspGWI ACGGA 3 cut(s) 145, 188, 1625
TspRI CASTG 5 cut(s) 547, 741, 1062, 1240, 1519
Van91I CCANNNNNTGG 1 cut(s) 473
VpaK11BI GGWCC 6 cut(s) 500, 533, 589, 1049, 1239, 1337
XapI RAATTY 3 cut(s) 730, 1714, 1867
XceI RCATGY 1 cut(s) 937
XmiI GTMKAC 2 cut(s) 599, 1816
XmnI GAANNNNTTC 1 cut(s) 1350
XspI CTAG 2 cut(s) 300, 1467
ZraI GACGTC 1 cut(s) 597
ZrmI AGTACT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.