MD02G1016100.v1.1

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007815
Physical Location & Seq
Reverse (-)
1221416 .. 1227411
5996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1016100.v1.1.491

Sequence Viewer

Length: 2193 bp
ATGGGTAGTAATGGTACTGAACATATTCCCATGGATGAGCAGGTTGAAGGGTCTGAAGCCACTATTGAGATTAAAATTAAAACATTGGATTCACAAACCTATACTCTCAGGGTGGATAAACAGATGCCAGTTCCTGCTTTGAAAGAACAGATTGCTTCTGTCACCGGTGTGCTATCAGAACAACAACGACTGATATGCCGAGGAAAAGTTCTAAAGGATGATCAGCTTCTCTCTGCCTATCATGTTGAAGATGGCCACACCTTACATTTGGTTTTGAGACAACCTATTCCACCATCACCTGAAGGATTACCAGATCATCCAGGAACTAATCCGGCTTCAAGTACAAGCCGTCATGTTGGCCCTGGTGTTGTCATTGAAACTTTCAGCATGCCTGTTCAAGGGGATGGGTTTGCACCTGAAGTAAATCGGATAATCTCTGCTGTTCTTGGCTCTATAGGAATGCCAAATATTGCTGGTGGCAGTGAAGGGATCGAAGTCAGGGAACATGGGTCACAGAGGCCAGAAAGGACATCGGGTTTGGGGGGTATGTTTGATTTCTCCCAATTTCATTCTGAACAAGCTGCCACAAGGGGTCCATCTGACAGATCAAATGGGACTTTTATTCATTCAACATCATTTCCTTTGGGGCCTCATCCACCTCTGGTAATTCCTGATTCTTTGACCACTTTAACCCAATATCTGAGTCATATGAGGTGTGAATTTGAGGCCATTGCTCATTTAACAACAGGCATAGATGCGGGAAGCAATCAAGCAGCTGCTACTCATAGGACGGAAGAAAGCTCCAATTCCTCCTCACGTTCAGGGACAAGGCAAGAGGGGCTCCCCACTCCTGCATCATTGGCAGAAGTCATGCGTTCTACTCGACAATTACTCGCTGAACAAGTTGGAGAATCCTTACTTCAATTTGCAAGTCAACTGGATAATCAAGTGAATGTGACTGATCCGGCAGCACGGTTGAGCACCCAAGCTAGTGCATCGAGAAATGGAGCTTTATTTCATAACCTAGGTGCCTTTTTACTTGAACTTGGTCGTACAACCATGACATTGCAAATGGGTCAAGCACCGTCTGACGCGGTGGTTAATGCTGGACCTGCTGTTTTCATATCCCCTACTGGCCCTAATCCCATTATGGTTCAGCCTCTTCCTTTCCAATCTGGAACGAACTTTGGTGCCATCCCCATGGGAGCTGTGCAGCCTGGTTCTGGTCTTGGTAGTGGACTTGGTACCGGTTTTCTTCCAAGGCGTATTGATATACAAATACGAAGAGGTTCGTCAGCAACCACACCCAATGCCACTCGAGAGGAAAATGGGGAGACTCATCAGCCGTCAGGGCAAAGAAACCCAGCAACAAGTTCTGGTGGTGAAGATCCTACTAATCAAGCCACTTCAAGGGTCCCAGGTGGTTCAGCTTTTGCTGGAGATCCGGCTGTAAGGATAGTGCCAATGAGGACCATGGTTGCAACAGTCCCTGCTTCCCTCAGTCGCCAACCATCAGATTCTTCTGGTATTTCGGGAGGGTTATACTATCCACTGCTTGGAAGGTTTCAGCATGTAGCTTCAGGGAATGTGAGTAGTGAACGAGTAACTCCAGCGTCTAGAGATCATCACCCTGCCAATCTCCATACTGATCAGCAGTCTTCTGAATCTGCAGCAGAACAGCAAAATGCTGCAGATTCTTCTGCTGCAGATTCTGCAAGAGATGGATCAGCTCCCAATGTCAGACGGCCATCTATCTCACGAAGTGTCAGTATCAATATTTTATCAGCTGGTGGAACCCCAAACAGCCAAGACTCTGAGAGACAGGTCCCAAGCAGTATATTACAGTTTATAAGGACCCTCTTTCCGAATGGTGAACTTCATGTAGAAGATGGCAGTGCTGAGGGAGTGATTGCAGGTTCTGTACCAGATCAGGCAAGGACATCTAGTGGTGGTGTAGCTGCACCGGAAGCAGAGCCAAGGGCCACCGATGAAGGAATATTTTTGTCAAATTTACTTCACCAAATCATGCCATTCATATCTCAAGCCACAGGGGGAGAGCCAGGAAATTCTTCTGAGCACAGAATGGCCCAAGATTCTTCCACCAGGGCTGAAACGTCCAATGTTGGGTCATCCCATCAGCGTAGTGATTCTGAACCAGATCCTCCAACTTCAAAACGCCAAAAGACGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001775 GO:0001822 GO:0002020 GO:0002253 GO:0002376 GO:0002429 GO:0002474 GO:0002682 GO:0002684 GO:0002757 GO:0002764 GO:0002768 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010604 GO:0010605 GO:0010941 GO:0012501 GO:0016020 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019882 GO:0019899 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030101 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0030544 GO:0031072 GO:0031323 GO:0031324 GO:0031325 GO:0031329 GO:0031330 GO:0031331 GO:0031593 GO:0031625 GO:0031647 GO:0031974 GO:0031981 GO:0031982 GO:0032268 GO:0032269 GO:0032270 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036506 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042802 GO:0042981 GO:0043021 GO:0043022 GO:0043066 GO:0043067 GO:0043069 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043230 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045321 GO:0045732 GO:0045861 GO:0045862 GO:0045995 GO:0046649 GO:0048002 GO:0048232 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051787 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0060548 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070059 GO:0070062 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0080134 GO:0080135 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1901800 GO:1903046 GO:1903050 GO:1903051 GO:1903052 GO:1903362 GO:1903363 GO:1903364 GO:1903561 GO:1904292 GO:1904294 GO:1904378 GO:1905897 GO:1905898 GO:1990381 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

731

Amino Acids

76.74

Weight (kDa)

5.5

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 22 - 92 7.9e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 24 - 94 1.5e-20 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1850
Acc36I ACCTGC 3 cut(s) 31, 1120, 1904
Acc65I GGTACC 1 cut(s) 1244
AccB1I GGYRCC 3 cut(s) 1028, 1190, 1244
AccB7I CCANNNNNTGG 1 cut(s) 1556
AccII CGCG 1 cut(s) 1094
AciI CCGC 2 cut(s) 758, 1094
AclWI GGATC 6 cut(s) 497, 956, 1382, 1436, 1732, 2153
AcoI YGGCCR 2 cut(s) 253, 1745
AcsI RAATTY 3 cut(s) 719, 2008, 2065
AcuI CTGAAG 4 cut(s) 75, 321, 438, 1563
AdeI CACNNNGTG 1 cut(s) 1763
AfaI GTAC 5 cut(s) 16, 343, 1054, 1246, 1923
AfiI CCNNNNNNNGG 6 cut(s) 398, 1223, 1410, 1556, 2124, 2185
AgeI ACCGGT 2 cut(s) 164, 1247
AjnI CCWGG 6 cut(s) 319, 361, 1216, 1417, 2059, 2102
AleI CACNNNNGTG 1 cut(s) 167
Alw21I GWGCWC 2 cut(s) 983, 2079
Alw26I GTCTC 3 cut(s) 271, 1328, 1813
AlwI GGATC 6 cut(s) 497, 956, 1382, 1436, 1732, 2153
AlwNI CAGNNNCTG 4 cut(s) 134, 1490, 1712, 1919
Ama87I CYCGRG 1 cut(s) 1317
AoxI GGCC 9 cut(s) 253, 358, 518, 647, 726, 1135, 1745, 1980, 2085
ApeKI GCWGC 9 cut(s) 581, 773, 776, 968, 1213, 1670, 1688, 1703, 1958
ApoI RAATTY 3 cut(s) 719, 2008, 2065
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
AsiGI ACCGGT 2 cut(s) 164, 1247
Asp700I GAANNNNTTC 3 cut(s) 24, 1288, 2068
Asp718I GGTACC 1 cut(s) 1244
AspA2I CCTAGG 1 cut(s) 1024
AsuHPI GGTGA 6 cut(s) 154, 288, 1394, 1619, 1883, 2009
AvaI CYCGRG 1 cut(s) 1317
AvaII GGWCC 6 cut(s) 593, 1109, 1414, 1470, 1825, 1854
AvrII CCTAGG 1 cut(s) 1024
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 3 cut(s) 1028, 1190, 1244
BanII GRGCYC 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 1650
Bbv12I GWGCWC 2 cut(s) 983, 2079
BbvCI CCTCAGC 1 cut(s) 1899
BbvI GCAGC 9 cut(s) 568, 763, 785, 980, 1225, 1675, 1682, 1690, 1945
BceAI ACGGC 3 cut(s) 333, 1330, 1760
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BciT130I CCWGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
BclI TGATCA 2 cut(s) 220, 1648
BcoDI GTCTC 3 cut(s) 271, 1328, 1813
BfaI CTAG 4 cut(s) 990, 1025, 1617, 1944
BfmI CTRYAG 4 cut(s) 453, 1668, 1689, 1704
BfuAI ACCTGC 3 cut(s) 31, 1120, 1904
BglI GCCNNNNNGGC 1 cut(s) 1351
BisI GCNGC 9 cut(s) 582, 774, 777, 969, 1214, 1671, 1689, 1704, 1959
BlnI CCTAGG 1 cut(s) 1024
BlsI GCNGC 9 cut(s) 583, 775, 778, 970, 1215, 1672, 1690, 1705, 1960
Bme1390I CCNGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
Bme18I GGWCC 6 cut(s) 593, 1109, 1414, 1470, 1825, 1854
BmeT110I CYCGRG 1 cut(s) 1317
BmrFI CCNGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
BmsI GCATC 4 cut(s) 114, 745, 863, 1004
BpiI GAAGAC 1 cut(s) 1650
BpmI CTGGAG 2 cut(s) 1458, 1593
Bpu10I CCTNAGC 1 cut(s) 1899
BpuEI CTTGAG 1 cut(s) 2025
BsaWI WCCGGW 3 cut(s) 164, 1247, 1963
Bsc4I CCNNNNNNNGG 6 cut(s) 398, 1223, 1410, 1556, 2124, 2185
Bse118I RCCGGY 2 cut(s) 164, 1247
Bse1I ACTGG 3 cut(s) 128, 942, 1138
Bse3DI GCAATG 2 cut(s) 729, 1064
BseBI CCWGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
BseGI GGATG 7 cut(s) 40, 223, 316, 409, 652, 1194, 2129
BseLI CCNNNNNNNGG 6 cut(s) 398, 1223, 1410, 1556, 2124, 2185
BseMI GCAATG 2 cut(s) 729, 1064
BseMII CTCAG 6 cut(s) 121, 692, 1513, 1806, 1890, 2064
BseNI ACTGG 3 cut(s) 128, 942, 1138
BseRI GAGGAG 1 cut(s) 802
BseXI GCAGC 9 cut(s) 568, 763, 785, 980, 1225, 1675, 1682, 1690, 1945
BseYI CCCAGC 1 cut(s) 1363
BsgI GTGCAG 2 cut(s) 1232, 1944
Bsh1236I CGCG 1 cut(s) 1094
BshFI GGCC 9 cut(s) 255, 360, 520, 649, 728, 1137, 1747, 1982, 2087
BshNI GGYRCC 3 cut(s) 1028, 1190, 1244
BshTI ACCGGT 2 cut(s) 164, 1247
BsiHKAI GWGCWC 2 cut(s) 983, 2079
BsiHKCI CYCGRG 1 cut(s) 1317
BsiSI CCGG 6 cut(s) 165, 332, 965, 1248, 1445, 1964
BslFI GGGAC 5 cut(s) 628, 838, 1400, 1472, 1811
BslI CCNNNNNNNGG 6 cut(s) 398, 1223, 1410, 1556, 2124, 2185
BsmAI GTCTC 3 cut(s) 271, 1328, 1813
BsmFI GGGAC 5 cut(s) 628, 838, 1400, 1472, 1811
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 9 cut(s) 255, 360, 520, 649, 728, 1137, 1747, 1982, 2087
BsoBI CYCGRG 1 cut(s) 1317
Bsp1286I GDGCHC 3 cut(s) 843, 983, 2079
Bsp19I CCATGG 3 cut(s) 30, 1200, 1473
BspACI CCGC 2 cut(s) 758, 1094
BspANI GGCC 9 cut(s) 255, 360, 520, 649, 728, 1137, 1747, 1982, 2087
BspCNI CTCAG 6 cut(s) 120, 693, 1512, 1807, 1891, 2065
BspFNI CGCG 1 cut(s) 1094
BspMAI CTGCAG 3 cut(s) 1672, 1693, 1708
BspMI ACCTGC 3 cut(s) 31, 1120, 1904
BspPI GGATC 6 cut(s) 497, 956, 1382, 1436, 1732, 2153
BspT107I GGYRCC 3 cut(s) 1028, 1190, 1244
BsrDI GCAATG 2 cut(s) 729, 1064
BsrFI RCCGGY 2 cut(s) 164, 1247
BsrI ACTGG 3 cut(s) 128, 942, 1138
BssAI RCCGGY 2 cut(s) 164, 1247
BssT1I CCWWGG 6 cut(s) 30, 1024, 1200, 1259, 1473, 1976
Bst2UI CCWGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
Bst4CI ACNGT 4 cut(s) 975, 1086, 1486, 1845
Bst6I CTCTTC 2 cut(s) 1167, 1279
BstAPI GCANNNNNTGC 1 cut(s) 1712
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 6 cut(s) 107, 701, 1499, 1815, 1899, 2073
BstDSI CCRYGG 3 cut(s) 30, 1200, 1473
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 7 cut(s) 40, 223, 316, 409, 652, 1194, 2129
BstFNI CGCG 1 cut(s) 1094
BstMAI GTCTC 3 cut(s) 271, 1328, 1813
BstMWI GCNNNNNNNGC 6 cut(s) 838, 860, 1112, 1351, 1712, 1967
BstNI CCWGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
BstNSI RCATGY 2 cut(s) 391, 1574
BstSCI CCNGG 6 cut(s) 319, 361, 1216, 1417, 2059, 2102
BstSFI CTRYAG 4 cut(s) 453, 1668, 1689, 1704
BstUI CGCG 1 cut(s) 1094
BstV1I GCAGC 9 cut(s) 568, 763, 785, 980, 1225, 1675, 1682, 1690, 1945
BstV2I GAAGAC 1 cut(s) 1650
BstX2I RGATCY 3 cut(s) 1387, 1441, 2158
BstXI CCANNNNNNTGG 1 cut(s) 1201
BstYI RGATCY 3 cut(s) 1387, 1441, 2158
BsuRI GGCC 9 cut(s) 255, 360, 520, 649, 728, 1137, 1747, 1982, 2087
BtgI CCRYGG 3 cut(s) 30, 1200, 1473
BtsCI GGATG 7 cut(s) 40, 223, 316, 409, 652, 1194, 2129
BtsI GCAGTG 3 cut(s) 487, 1550, 1900
BtsIMutI CAGTG 3 cut(s) 487, 1550, 1900
BveI ACCTGC 3 cut(s) 31, 1120, 1904
Cac8I GCNNGC 1 cut(s) 389
CaiI CAGNNNCTG 4 cut(s) 134, 1490, 1712, 1919
Cfr10I RCCGGY 2 cut(s) 164, 1247
CseI GACGC 2 cut(s) 1100, 1602
Csp6I GTAC 5 cut(s) 15, 342, 1053, 1245, 1922
CspAI ACCGGT 2 cut(s) 164, 1247
CviQI GTAC 5 cut(s) 15, 342, 1053, 1245, 1922
DdeI CTNAG 6 cut(s) 107, 701, 1499, 1815, 1899, 2073
DraIII CACNNNGTG 1 cut(s) 1763
EaeI YGGCCR 2 cut(s) 253, 1745
Eam1104I CTCTTC 2 cut(s) 1167, 1279
EarI CTCTTC 2 cut(s) 1167, 1279
Eco130I CCWWGG 6 cut(s) 30, 1024, 1200, 1259, 1473, 1976
Eco24I GRGCYC 1 cut(s) 843
Eco47I GGWCC 6 cut(s) 593, 1109, 1414, 1470, 1825, 1854
Eco57I CTGAAG 4 cut(s) 75, 321, 438, 1563
Eco88I CYCGRG 1 cut(s) 1317
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoO109I RGGNCCY 4 cut(s) 647, 1414, 1825, 1854
EcoRII CCWGG 6 cut(s) 319, 361, 1216, 1417, 2059, 2102
EcoT14I CCWWGG 6 cut(s) 30, 1024, 1200, 1259, 1473, 1976
EcoT38I GRGCYC 1 cut(s) 843
ErhI CCWWGG 6 cut(s) 30, 1024, 1200, 1259, 1473, 1976
FaqI GGGAC 5 cut(s) 628, 838, 1400, 1472, 1811
FauI CCCGC 1 cut(s) 751
FauNDI CATATG 1 cut(s) 708
FbaI TGATCA 2 cut(s) 220, 1648
Fnu4HI GCNGC 9 cut(s) 582, 774, 777, 969, 1214, 1671, 1689, 1704, 1959
FokI GGATG 7 cut(s) 47, 230, 303, 416, 639, 1181, 2116
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 9 cut(s) 582, 774, 777, 969, 1214, 1671, 1689, 1704, 1959
FspBI CTAG 4 cut(s) 990, 1025, 1617, 1944
GluI GCNGC 9 cut(s) 582, 774, 777, 969, 1214, 1671, 1689, 1704, 1959
GsaI CCCAGC 1 cut(s) 1367
GsuI CTGGAG 2 cut(s) 1458, 1593
HaeIII GGCC 9 cut(s) 255, 360, 520, 649, 728, 1137, 1747, 1982, 2087
HapII CCGG 6 cut(s) 165, 332, 965, 1248, 1445, 1964
HgaI GACGC 2 cut(s) 1100, 1602
HincII GTYRAC 1 cut(s) 935
HindII GTYRAC 1 cut(s) 935
HpaII CCGG 6 cut(s) 165, 332, 965, 1248, 1445, 1964
HphI GGTGA 6 cut(s) 154, 288, 1394, 1619, 1883, 2009
Hpy166II GTNNAC 4 cut(s) 935, 1238, 1598, 1874
Hpy188III TCNNGA 7 cut(s) 671, 999, 1176, 1319, 1533, 1617, 1758
Hpy8I GTNNAC 4 cut(s) 935, 1238, 1598, 1874
HpyAV CCTTC 5 cut(s) 41, 296, 479, 1554, 1985
HpyCH4III ACNGT 4 cut(s) 975, 1086, 1486, 1845
HpyCH4IV ACGT 2 cut(s) 817, 2114
HpyF10VI GCNNNNNNNGC 6 cut(s) 838, 860, 1112, 1351, 1712, 1967
HpyF3I CTNAG 6 cut(s) 107, 701, 1499, 1815, 1899, 2073
HpySE526I ACGT 2 cut(s) 817, 2114
KflI GGGWCCC 1 cut(s) 1414
KpnI GGTACC 1 cut(s) 1248
Ksp22I TGATCA 2 cut(s) 220, 1648
LmnI GCTCC 5 cut(s) 806, 846, 1007, 1205, 1735
Lsp1109I GCAGC 9 cut(s) 568, 763, 785, 980, 1225, 1675, 1682, 1690, 1945
LweI GCATC 4 cut(s) 114, 745, 863, 1004
MaeI CTAG 4 cut(s) 990, 1025, 1617, 1944
MaeII ACGT 2 cut(s) 817, 2114
MaeIII GTNAC 4 cut(s) 160, 510, 955, 1603
MflI RGATCY 3 cut(s) 1387, 1441, 2158
MhlI GDGCHC 3 cut(s) 843, 983, 2079
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 9 cut(s) 75, 563, 666, 719, 805, 887, 923, 2008, 2065
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 3 cut(s) 712, 1330, 1805
MmeI TCCRAC 2 cut(s) 886, 2189
Mox20I TGGCCA 1 cut(s) 255
MroXI GAANNNNTTC 3 cut(s) 24, 1288, 2068
MscI TGGCCA 1 cut(s) 255
MseI TTAA 5 cut(s) 72, 78, 689, 740, 1101
MslI CAYNNNNRTG 2 cut(s) 167, 1199
Msp20I TGGCCA 1 cut(s) 255
MspA1I CMGCKG 2 cut(s) 776, 1787
MspI CCGG 6 cut(s) 165, 332, 965, 1248, 1445, 1964
MspR9I CCNGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
Mva1269I GAATGC 1 cut(s) 465
MvaI CCWGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
MvnI CGCG 1 cut(s) 1094
MwoI GCNNNNNNNGC 6 cut(s) 838, 860, 1112, 1351, 1712, 1967
NcoI CCATGG 3 cut(s) 30, 1200, 1473
NdeI CATATG 1 cut(s) 708
NmeAIII GCCGAG 1 cut(s) 224
NmuCI GTSAC 3 cut(s) 160, 510, 955
NspI RCATGY 2 cut(s) 391, 1574
OliI CACNNNNGTG 1 cut(s) 167
PaeI GCATGC 1 cut(s) 391
PaeR7I CTCGAG 1 cut(s) 1317
PctI GAATGC 1 cut(s) 465
PdmI GAANNNNTTC 3 cut(s) 24, 1288, 2068
PfeI GAWTC 9 cut(s) 89, 674, 911, 1517, 1664, 1694, 1709, 2093, 2147
PflMI CCANNNNNTGG 1 cut(s) 1556
PfoI TCCNGGA 1 cut(s) 319
PinAI ACCGGT 2 cut(s) 164, 1247
PkrI GCNGC 9 cut(s) 583, 775, 778, 970, 1215, 1672, 1690, 1705, 1960
PleI GAGTC 3 cut(s) 711, 1330, 1805
PpsI GAGTC 3 cut(s) 711, 1330, 1805
PpuMI RGGWCCY 3 cut(s) 1414, 1825, 1854
PsiI TTATAA 1 cut(s) 1850
Psp5II RGGWCCY 3 cut(s) 1414, 1825, 1854
Psp6I CCWGG 6 cut(s) 319, 361, 1216, 1417, 2059, 2102
PspFI CCCAGC 1 cut(s) 1363
PspGI CCWGG 6 cut(s) 319, 361, 1216, 1417, 2059, 2102
PspPPI RGGWCCY 3 cut(s) 1414, 1825, 1854
PstI CTGCAG 3 cut(s) 1672, 1693, 1708
PstNI CAGNNNCTG 4 cut(s) 134, 1490, 1712, 1919
PsuI RGATCY 3 cut(s) 1387, 1441, 2158
PvuII CAGCTG 2 cut(s) 776, 1787
RsaI GTAC 5 cut(s) 16, 343, 1054, 1246, 1923
RsaNI GTAC 5 cut(s) 15, 342, 1053, 1245, 1922
RseI CAYNNNNRTG 2 cut(s) 167, 1199
SaqAI TTAA 5 cut(s) 72, 78, 689, 740, 1101
SatI GCNGC 9 cut(s) 582, 774, 777, 969, 1214, 1671, 1689, 1704, 1959
SchI GAGTC 3 cut(s) 712, 1330, 1805
ScrFI CCNGG 6 cut(s) 321, 363, 1218, 1419, 2061, 2104
SduI GDGCHC 3 cut(s) 843, 983, 2079
SfaNI GCATC 4 cut(s) 114, 745, 863, 1004
SfcI CTRYAG 4 cut(s) 453, 1668, 1689, 1704
Sfr274I CTCGAG 1 cut(s) 1317
SgrAI CRCCGGYG 1 cut(s) 164
SinI GGWCC 6 cut(s) 593, 1109, 1414, 1470, 1825, 1854
SlaI CTCGAG 1 cut(s) 1317
SmiMI CAYNNNNRTG 2 cut(s) 167, 1199
SmlI CTYRAG 2 cut(s) 1317, 2040
SmoI CTYRAG 2 cut(s) 1317, 2040
SphI GCATGC 1 cut(s) 391
Sse9I AATT 9 cut(s) 75, 563, 666, 719, 805, 887, 923, 2008, 2065
SsiI CCGC 2 cut(s) 758, 1094
SspI AATATT 3 cut(s) 469, 1777, 1998
SspMI CTAG 4 cut(s) 990, 1025, 1617, 1944
StyD4I CCNGG 6 cut(s) 319, 361, 1216, 1417, 2059, 2102
StyI CCWWGG 6 cut(s) 30, 1024, 1200, 1259, 1473, 1976
TaaI ACNGT 4 cut(s) 975, 1086, 1486, 1845
TaiI ACGT 2 cut(s) 820, 2117
TaqI TCGA 4 cut(s) 492, 883, 998, 1318
TasI AATT 9 cut(s) 75, 563, 666, 719, 805, 887, 923, 2008, 2065
TatI WGTACW 1 cut(s) 341
TfiI GAWTC 9 cut(s) 89, 674, 911, 1517, 1664, 1694, 1709, 2093, 2147
Tru1I TTAA 5 cut(s) 72, 78, 689, 740, 1101
Tru9I TTAA 5 cut(s) 72, 78, 689, 740, 1101
TscAI CASTG 3 cut(s) 487, 1557, 1900
TseFI GTSAC 3 cut(s) 160, 510, 955
TseI GCWGC 9 cut(s) 581, 773, 776, 968, 1213, 1670, 1688, 1703, 1958
Tsp45I GTSAC 3 cut(s) 160, 510, 955
TspDTI ATGAA 7 cut(s) 557, 614, 1007, 1111, 1868, 2004, 2023
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 3 cut(s) 487, 1557, 1900
Van91I CCANNNNNTGG 1 cut(s) 1556
VpaK11BI GGWCC 6 cut(s) 593, 1109, 1414, 1470, 1825, 1854
XagI CCTNNNNNAGG 1 cut(s) 396
XapI RAATTY 3 cut(s) 719, 2008, 2065
XbaI TCTAGA 1 cut(s) 1616
XceI RCATGY 2 cut(s) 391, 1574
XhoI CTCGAG 1 cut(s) 1317
XmaJI CCTAGG 1 cut(s) 1024
XmnI GAANNNNTTC 3 cut(s) 24, 1288, 2068
XspI CTAG 4 cut(s) 990, 1025, 1617, 1944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.