FvH4_1g01820

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007815
Physical Location & Seq
Reverse (-)
951176 .. 956877
5702 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01820.t2

Sequence Viewer

Length: 2136 bp
ATGGGAAGTACTGGCGCTGAAGATATTTTGATGAGTGAGCCCACGATAGAGATAAAAGTAAAGACATTGGATTCACAAACTTATACTCTCAAAGTGGATAAAGAGATGCCAGTTCCTATTTTGAAAGAACAGATTGCCTCTGTTACTGGTGTGCTGTCAGAGCAACAACGGCTAATATGCAGAGGAAAAGTTCTCAAGGATGATCAGCTTCTCTCCGCCTATCATGTTGAGGATGGCCACACCTTACATTTGGTTGTCAGACAACCTATTCCACCATCATCTGAGGGACTAGCAGATCATTCAGCAACTGATCCTGCTTCAAGTACAAGCCGTGGTCGATCAAGTGTCTTCATTGAAAGTTTCAGTTTGCCTGTTGGGGGGGATGTTCAACCAGATTATAGTCGGTTTCTCTCCGCTGTTCTGGGCTCTGTCGGAATTCCAAATATTGCAAGTGCCAGTGAAGGGGTTGATGTCATGTTTCCTCCTGGGACAAATAGGCCAGAAAGGACATCAGGTTTGAGTGGTTTGTTTGGTTTAACCCAACTTCTTTCTGAGCAAACTACCGCAAGGGCTCAACCTGATGGATCAAATGACACTTTTGGTCAGTCCGCGGCATTTTCATTGGGGAATCTGCCACCTCTGGTGATTCCTGATTCGTTGAATACTTTATCTCGATATCTGAGTCATATAAGGCGCGACTTTCAGGCAATTGCCAGAGATGGAGGACAGAGTCAAGCAGCTATTCATAGGAATGACGGAAGTTCGAATTCGTCATCTGGTTCTGGAATAAGACGAGAGGGCCCCCCCTCACCTGCATCATTGGCAGAAGTCATCCGTACTGCTAGACTATTACTCATTGAACAAGTTGGAGACTCTCTACTTCAACTTGCAAGCCAGTTGGAGAATCAAGCAAATGTGACGGATCACTCAGTAAGGAGGCGAATCCAAGCCAGTTCATTGAGTACCGGAGCCGTGATTCATAATTTAGGTGCCTGTTTACTTGAACTTGGCCGTACAATGATGACAGTGCAAATGGGCCTAACACCGTCTGATGCAGTGGTTAATGCTGGACCTGCAGTTTTCATATCCCCGACGGGTCCTAACCCTATAATGGTTCAGCCTCTTCCATTTCAATCAGGAATGAGTGTAGGTGCCATGCCCATGGGAGCTCTGCAGCCTGGTTCTGGTCTACTGAATGGATTTGGTTCTGGTTTTCATCCAAGGCGTATTGATATACAAATACGAAGAGGTTCACCAACGACCACTGCCAATGTCAATCGAGAGGAGCATCTCGATCTTCAGCAACCTTTACAAAGAAACGCAGCACCAAGTTCAGGTGGTGAAAATCCTGCCAATCAAACAGCGCCAAGTATCTCAGATGGTTCAGCTTCTGCTGGAGATTTGGGTGTAAGGCCAAGGGTAGTGCCAATCAGGACCGTACTTGGCAGTCGCATACCATCGGAGTCTGCTGGTAGTTCATTTGGGTTATACTATCCATTTCTTGGAAGGTCTCAAAATGTAGCTTCTGGAAATGTGAATGCTGAGCAAGTATCTCAAGCATCTGGTGATCGTCATCCTGCCGGCCTTCATACTGATCGGCAATCTTCAGAAAACATTACAGATCCTGCAAGAGAGGGATCAACTCCCTATATAAGGCAACCAGATCCCCCCATTGGACGAAGTGTTAGTATCAACCTTTTTTCATCTGGTGGAACCCAAAACGATCAAGAATCTGAGAGACAGGTCCCAAGCGGTATTTTAAATATACTAAGGGCTCTCTTTCCTGGTGATGAAATTCGCGTAGAAGATGGCAGTGTACAAGGAATGAATTCTAGTTCTGTATCAGATCAGGCAAGGACACCTAGTGGTGTTGCTGTTGCTCCAGAAGCTGAACTGAGGGTTACTGAAGAAGGATTATTTTTGTCTAATTTGCTTCATCAAGTAATGCCATTGATATCTCAAGCAGCAACTAGAGAGCCAGGTCATGTACCTGCAGAAGCGGAAAATTCTTCTGAGCACACAATGGCCCAAGACTCTTCCACCGTGACGGAAAATTCCAATGTTGGGACATCGCGTCGGCATAGTCAGAGTGATTGTGACGAGCCTCCTAGTTCAAAACGTCAAAAGAACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001775 GO:0001822 GO:0002020 GO:0002253 GO:0002376 GO:0002429 GO:0002474 GO:0002682 GO:0002684 GO:0002757 GO:0002764 GO:0002768 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010604 GO:0010605 GO:0010941 GO:0012501 GO:0016020 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019882 GO:0019899 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030101 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0030544 GO:0031072 GO:0031323 GO:0031324 GO:0031325 GO:0031329 GO:0031330 GO:0031331 GO:0031593 GO:0031625 GO:0031647 GO:0031974 GO:0031981 GO:0031982 GO:0032268 GO:0032269 GO:0032270 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036506 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042802 GO:0042981 GO:0043021 GO:0043022 GO:0043066 GO:0043067 GO:0043069 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043230 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045321 GO:0045732 GO:0045861 GO:0045862 GO:0045995 GO:0046649 GO:0048002 GO:0048232 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051787 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0060548 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070059 GO:0070062 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0080134 GO:0080135 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1901800 GO:1903046 GO:1903050 GO:1903051 GO:1903052 GO:1903362 GO:1903363 GO:1903364 GO:1903561 GO:1904292 GO:1904294 GO:1904378 GO:1905897 GO:1905898 GO:1990381 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

712

Amino Acids

75.19

Weight (kDa)

5.41

Isoelectric Point (pI)

61.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 16 - 86 8.5e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 18 - 88 2.9e-20 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 820
Acc36I ACCTGC 3 cut(s) 820, 1081, 1999
AccB1I GGYRCC 2 cut(s) 989, 1151
AccB7I CCANNNNNTGG 1 cut(s) 1502
AccI GTMKAC 1 cut(s) 1189
AccII CGCG 4 cut(s) 611, 696, 1800, 2074
AciI CCGC 7 cut(s) 216, 414, 564, 609, 611, 1752, 2000
AclWI GGATC 6 cut(s) 305, 592, 930, 1616, 1645, 1658
AcoI YGGCCR 2 cut(s) 235, 1009
AcsI RAATTY 6 cut(s) 435, 766, 1794, 1828, 2005, 2053
AcuI CTGAAG 4 cut(s) 39, 1283, 1590, 1926
AdeI CACNNNGTG 1 cut(s) 1865
AfaI GTAC 8 cut(s) 10, 325, 838, 964, 1015, 1440, 1818, 1989
AfiI CCNNNNNNNGG 9 cut(s) 377, 462, 1111, 1184, 1334, 1502, 1653, 1673, 2064
AhdI GACNNNNNGTC 1 cut(s) 2073
AjnI CCWGG 4 cut(s) 484, 1177, 1783, 1978
AluBI AGCT 6 cut(s) 208, 740, 1169, 1388, 1523, 1889
AluI AGCT 6 cut(s) 208, 740, 1169, 1388, 1523, 1889
Alw21I GWGCWC 2 cut(s) 1171, 2019
Alw26I GTCTC 3 cut(s) 864, 1515, 1732
AlwI GGATC 6 cut(s) 305, 592, 930, 1616, 1645, 1658
AlwNI CAGNNNCTG 4 cut(s) 308, 1391, 1625, 1889
AoxI GGCC 8 cut(s) 235, 497, 799, 1009, 1036, 1412, 1582, 2025
ApaI GGGCCC 1 cut(s) 803
ApeKI GCWGC 4 cut(s) 737, 1174, 1322, 1964
ApoI RAATTY 6 cut(s) 435, 766, 1794, 1828, 2005, 2053
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
Asp700I GAANNNNTTC 2 cut(s) 1249, 1828
AspLEI GCGC 3 cut(s) 17, 696, 1366
AspS9I GGNCC 8 cut(s) 799, 800, 1036, 1070, 1097, 1434, 1744, 2026
AsuHPI GGTGA 6 cut(s) 655, 801, 1245, 1352, 1577, 1799
AsuII TTCGAA 1 cut(s) 764
AvaII GGWCC 4 cut(s) 1070, 1097, 1434, 1744
BaeGI GKGCMC 1 cut(s) 803
BalI TGGCCA 1 cut(s) 237
BanI GGYRCC 2 cut(s) 989, 1151
BanII GRGCYC 6 cut(s) 42, 428, 574, 803, 1171, 1777
BbsI GAAGAC 1 cut(s) 340
Bbv12I GWGCWC 2 cut(s) 1171, 2019
BbvI GCAGC 4 cut(s) 749, 1186, 1334, 1976
BccI CCATC 7 cut(s) 227, 283, 575, 713, 1373, 1465, 1802
BceAI ACGGC 4 cut(s) 185, 315, 956, 996
BciT130I CCWGG 4 cut(s) 486, 1179, 1785, 1980
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 3 cut(s) 864, 1515, 1732
BfaI CTAG 6 cut(s) 290, 843, 1833, 1863, 1971, 2109
BfmI CTRYAG 3 cut(s) 1074, 1172, 1992
BfoI RGCGCY 2 cut(s) 18, 1367
BfuAI ACCTGC 3 cut(s) 820, 1081, 1999
BisI GCNGC 5 cut(s) 612, 738, 1175, 1323, 1965
BlpI GCTNAGC 1 cut(s) 1542
BlsI GCNGC 5 cut(s) 613, 739, 1176, 1324, 1966
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 4 cut(s) 486, 1179, 1785, 1980
Bme18I GGWCC 4 cut(s) 1070, 1097, 1434, 1744
BmeRI GACNNNNNGTC 1 cut(s) 2073
BmgT120I GGNCC 8 cut(s) 799, 800, 1036, 1070, 1097, 1434, 1744, 2026
BmiI GGNNCC 8 cut(s) 801, 802, 970, 991, 1098, 1153, 1714, 1746
BmrFI CCNGG 4 cut(s) 486, 1179, 1785, 1980
BmsI GCATC 5 cut(s) 96, 824, 1042, 1297, 1568
BpiI GAAGAC 1 cut(s) 340
BpmI CTGGAG 2 cut(s) 1416, 1866
Bpu1102I GCTNAGC 1 cut(s) 1542
Bpu14I TTCGAA 1 cut(s) 764
BpuEI CTTGAG 3 cut(s) 179, 1539, 1944
BsaBI GATNNNNATC 1 cut(s) 1572
BsaI GGTCTC 1 cut(s) 1515
BsaJI CCNNGG 6 cut(s) 331, 485, 609, 1161, 1220, 1415
BsaWI WCCGGW 1 cut(s) 965
Bsc4I CCNNNNNNNGG 9 cut(s) 377, 462, 1111, 1184, 1334, 1502, 1653, 1673, 2064
Bse118I RCCGGY 1 cut(s) 1580
Bse1I ACTGG 6 cut(s) 16, 110, 151, 456, 895, 951
Bse8I GATNNNNATC 1 cut(s) 1572
BseBI CCWGG 4 cut(s) 486, 1179, 1785, 1980
BseDI CCNNGG 6 cut(s) 331, 485, 609, 1161, 1220, 1415
BseGI GGATG 6 cut(s) 205, 238, 388, 831, 1216, 1573
BseJI GATNNNNATC 1 cut(s) 1572
BseLI CCNNNNNNNGG 9 cut(s) 377, 462, 1111, 1184, 1334, 1502, 1653, 1673, 2064
BseMII CTCAG 9 cut(s) 273, 543, 671, 942, 1389, 1533, 1725, 1886, 2004
BseNI ACTGG 6 cut(s) 16, 110, 151, 456, 895, 951
BseRI GAGGAG 1 cut(s) 1298
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 4 cut(s) 749, 1186, 1334, 1976
Bsh1236I CGCG 4 cut(s) 611, 696, 1800, 2074
BshFI GGCC 8 cut(s) 237, 499, 801, 1011, 1038, 1414, 1584, 2027
BshNI GGYRCC 2 cut(s) 989, 1151
BsiHKAI GWGCWC 2 cut(s) 1171, 2019
BsiSI CCGG 2 cut(s) 966, 1581
BslFI GGGAC 4 cut(s) 300, 502, 1730, 2080
BslI CCNNNNNNNGG 9 cut(s) 377, 462, 1111, 1184, 1334, 1502, 1653, 1673, 2064
BsmAI GTCTC 3 cut(s) 864, 1515, 1732
BsmFI GGGAC 4 cut(s) 300, 502, 1730, 2080
BsmI GAATGC 1 cut(s) 1543
BsnI GGCC 8 cut(s) 237, 499, 801, 1011, 1038, 1414, 1584, 2027
Bso31I GGTCTC 1 cut(s) 1515
Bsp119I TTCGAA 1 cut(s) 764
Bsp120I GGGCCC 1 cut(s) 799
Bsp1286I GDGCHC 7 cut(s) 42, 428, 574, 803, 1171, 1777, 2019
Bsp1407I TGTACA 1 cut(s) 1816
Bsp1720I GCTNAGC 1 cut(s) 1542
Bsp19I CCATGG 1 cut(s) 1161
BspACI CCGC 7 cut(s) 216, 414, 564, 609, 611, 1752, 2000
BspANI GGCC 8 cut(s) 237, 499, 801, 1011, 1038, 1414, 1584, 2027
BspCNI CTCAG 9 cut(s) 274, 544, 672, 941, 1388, 1534, 1726, 1887, 2005
BspFNI CGCG 4 cut(s) 611, 696, 1800, 2074
BspLI GGNNCC 8 cut(s) 801, 802, 970, 991, 1098, 1153, 1714, 1746
BspMAI CTGCAG 3 cut(s) 1078, 1176, 1996
BspMI ACCTGC 3 cut(s) 820, 1081, 1999
BspPI GGATC 6 cut(s) 305, 592, 930, 1616, 1645, 1658
BspT104I TTCGAA 1 cut(s) 764
BspT107I GGYRCC 2 cut(s) 989, 1151
BspTNI GGTCTC 1 cut(s) 1515
BsrFI RCCGGY 1 cut(s) 1580
BsrGI TGTACA 1 cut(s) 1816
BsrI ACTGG 6 cut(s) 16, 110, 151, 456, 895, 951
BssAI RCCGGY 1 cut(s) 1580
BssECI CCNNGG 6 cut(s) 331, 485, 609, 1161, 1220, 1415
BssT1I CCWWGG 3 cut(s) 1161, 1220, 1415
Bst2UI CCWGG 4 cut(s) 486, 1179, 1785, 1980
Bst4CI ACNGT 4 cut(s) 1027, 1047, 1438, 2044
Bst6I CTCTTC 3 cut(s) 1128, 1240, 2041
BstAUI TGTACA 1 cut(s) 1816
BstBI TTCGAA 1 cut(s) 764
BstC8I GCNNGC 2 cut(s) 892, 1582
BstDSI CCRYGG 3 cut(s) 331, 609, 1161
BstENI CCTNNNNNAGG 1 cut(s) 1651
BstF5I GGATG 6 cut(s) 205, 238, 388, 831, 1216, 1573
BstFNI CGCG 4 cut(s) 611, 696, 1800, 2074
BstH2I RGCGCY 2 cut(s) 18, 1367
BstHHI GCGC 3 cut(s) 17, 696, 1366
BstMAI GTCTC 3 cut(s) 864, 1515, 1732
BstMWI GCNNNNNNNGC 5 cut(s) 160, 169, 821, 1073, 1886
BstNI CCWGG 4 cut(s) 486, 1179, 1785, 1980
BstSCI CCNGG 4 cut(s) 484, 1177, 1783, 1978
BstSFI CTRYAG 3 cut(s) 1074, 1172, 1992
BstSLI GKGCMC 1 cut(s) 803
BstUI CGCG 4 cut(s) 611, 696, 1800, 2074
BstV1I GCAGC 4 cut(s) 749, 1186, 1334, 1976
BstV2I GAAGAC 1 cut(s) 340
BstX2I RGATCY 2 cut(s) 1621, 1663
BstXI CCANNNNNNTGG 1 cut(s) 1162
BstYI RGATCY 2 cut(s) 1621, 1663
BsuRI GGCC 8 cut(s) 237, 499, 801, 1011, 1038, 1414, 1584, 2027
BtgI CCRYGG 3 cut(s) 331, 609, 1161
BtgZI GCGATG 1 cut(s) 2055
BtsCI GGATG 6 cut(s) 205, 238, 388, 831, 1216, 1573
BtsI GCAGTG 3 cut(s) 1062, 1263, 1819
BtsIMutI CAGTG 5 cut(s) 463, 1032, 1062, 1263, 1819
BveI ACCTGC 3 cut(s) 820, 1081, 1999
Cac8I GCNNGC 2 cut(s) 892, 1582
CaiI CAGNNNCTG 4 cut(s) 308, 1391, 1625, 1889
CfoI GCGC 3 cut(s) 17, 696, 1366
Cfr10I RCCGGY 1 cut(s) 1580
Cfr13I GGNCC 8 cut(s) 799, 800, 1036, 1070, 1097, 1434, 1744, 2026
Cfr42I CCGCGG 1 cut(s) 612
CseI GACGC 1 cut(s) 2063
Csp6I GTAC 8 cut(s) 9, 324, 837, 963, 1014, 1439, 1817, 1988
CviAII CATG 5 cut(s) 224, 475, 1156, 1162, 1985
CviQI GTAC 8 cut(s) 9, 324, 837, 963, 1014, 1439, 1817, 1988
DraI TTTAAA 1 cut(s) 1761
DraIII CACNNNGTG 1 cut(s) 1865
DriI GACNNNNNGTC 1 cut(s) 2073
EaeI YGGCCR 2 cut(s) 235, 1009
Eam1104I CTCTTC 3 cut(s) 1128, 1240, 2041
Eam1105I GACNNNNNGTC 1 cut(s) 2073
EarI CTCTTC 3 cut(s) 1128, 1240, 2041
EciI GGCGGA 1 cut(s) 205
Ecl136II GAGCTC 1 cut(s) 1169
Eco130I CCWWGG 3 cut(s) 1161, 1220, 1415
Eco24I GRGCYC 6 cut(s) 42, 428, 574, 803, 1171, 1777
Eco31I GGTCTC 1 cut(s) 1515
Eco32I GATATC 2 cut(s) 677, 1956
Eco47I GGWCC 4 cut(s) 1070, 1097, 1434, 1744
Eco53kI GAGCTC 1 cut(s) 1169
Eco57I CTGAAG 4 cut(s) 39, 1283, 1590, 1926
EcoICRI GAGCTC 1 cut(s) 1169
EcoNI CCTNNNNNAGG 1 cut(s) 1651
EcoO109I RGGNCCY 4 cut(s) 799, 800, 1097, 1744
EcoRI GAATTC 3 cut(s) 435, 766, 1828
EcoRII CCWGG 4 cut(s) 484, 1177, 1783, 1978
EcoRV GATATC 2 cut(s) 677, 1956
EcoT14I CCWWGG 3 cut(s) 1161, 1220, 1415
EcoT38I GRGCYC 6 cut(s) 42, 428, 574, 803, 1171, 1777
ErhI CCWWGG 3 cut(s) 1161, 1220, 1415
FaeI CATG 5 cut(s) 227, 478, 1159, 1165, 1988
FaqI GGGAC 4 cut(s) 300, 502, 1730, 2080
FatI CATG 5 cut(s) 223, 474, 1155, 1161, 1984
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 1189
Fnu4HI GCNGC 5 cut(s) 612, 738, 1175, 1323, 1965
FokI GGATG 6 cut(s) 212, 245, 395, 818, 1203, 1560
FriOI GRGCYC 6 cut(s) 42, 428, 574, 803, 1171, 1777
Fsp4HI GCNGC 5 cut(s) 612, 738, 1175, 1323, 1965
FspBI CTAG 6 cut(s) 290, 843, 1833, 1863, 1971, 2109
GlaI GCGC 3 cut(s) 16, 695, 1365
GluI GCNGC 5 cut(s) 612, 738, 1175, 1323, 1965
GsuI CTGGAG 2 cut(s) 1416, 1866
HaeII RGCGCY 2 cut(s) 18, 1367
HaeIII GGCC 8 cut(s) 237, 499, 801, 1011, 1038, 1414, 1584, 2027
HapII CCGG 2 cut(s) 966, 1581
HgaI GACGC 1 cut(s) 2063
HhaI GCGC 3 cut(s) 17, 696, 1366
Hin1II CATG 5 cut(s) 227, 478, 1159, 1165, 1988
Hin6I GCGC 3 cut(s) 15, 694, 1364
HinP1I GCGC 3 cut(s) 15, 694, 1364
HpaII CCGG 2 cut(s) 966, 1581
HphI GGTGA 6 cut(s) 655, 801, 1245, 1352, 1577, 1799
Hpy166II GTNNAC 4 cut(s) 998, 1190, 1253, 1817
Hpy8I GTNNAC 4 cut(s) 998, 1190, 1253, 1817
Hpy99I CGWCG 2 cut(s) 1096, 2079
HpyAV CCTTC 4 cut(s) 455, 1500, 1595, 1904
HpyCH4III ACNGT 4 cut(s) 1027, 1047, 1438, 2044
HpyCH4IV ACGT 1 cut(s) 2119
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 169, 821, 1073, 1886
HpySE526I ACGT 1 cut(s) 2119
Hsp92II CATG 5 cut(s) 227, 478, 1159, 1165, 1988
HspAI GCGC 3 cut(s) 15, 694, 1364
KroI GCCGGC 1 cut(s) 1580
KroNI GCCGGC 1 cut(s) 1582
Ksp22I TGATCA 1 cut(s) 202
KspI CCGCGG 1 cut(s) 612
LmnI GCTCC 4 cut(s) 968, 1166, 1285, 1885
Lsp1109I GCAGC 4 cut(s) 749, 1186, 1334, 1976
LweI GCATC 5 cut(s) 96, 824, 1042, 1297, 1568
MaeI CTAG 6 cut(s) 290, 843, 1833, 1863, 1971, 2109
MaeII ACGT 1 cut(s) 2119
MaeIII GTNAC 5 cut(s) 142, 916, 1900, 2044, 2096
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 2 cut(s) 1621, 1663
MhlI GDGCHC 7 cut(s) 42, 428, 574, 803, 1171, 1777, 2019
MlsI TGGCCA 1 cut(s) 237
MluCI AATT 9 cut(s) 435, 708, 766, 982, 1794, 1828, 1927, 2005, 2053
MluNI TGGCCA 1 cut(s) 237
MlyI GAGTC 5 cut(s) 691, 739, 866, 1472, 2027
MmeI TCCRAC 3 cut(s) 412, 847, 879
Mox20I TGGCCA 1 cut(s) 237
MroNI GCCGGC 1 cut(s) 1580
MroXI GAANNNNTTC 2 cut(s) 1249, 1828
MscI TGGCCA 1 cut(s) 237
MseI TTAA 3 cut(s) 536, 1062, 1760
MslI CAYNNNNRTG 2 cut(s) 750, 1160
Msp20I TGGCCA 1 cut(s) 237
MspA1I CMGCKG 2 cut(s) 416, 611
MspI CCGG 2 cut(s) 966, 1581
MspR9I CCNGG 4 cut(s) 486, 1179, 1785, 1980
MunI CAATTG 1 cut(s) 708
Mva1269I GAATGC 1 cut(s) 1543
MvaI CCWGG 4 cut(s) 486, 1179, 1785, 1980
MvnI CGCG 4 cut(s) 611, 696, 1800, 2074
MwoI GCNNNNNNNGC 5 cut(s) 160, 169, 821, 1073, 1886
NaeI GCCGGC 1 cut(s) 1582
NcoI CCATGG 1 cut(s) 1161
NgoMIV GCCGGC 1 cut(s) 1580
NlaIII CATG 5 cut(s) 227, 478, 1159, 1165, 1988
NlaIV GGNNCC 8 cut(s) 801, 802, 970, 991, 1098, 1153, 1714, 1746
NmuCI GTSAC 3 cut(s) 916, 2044, 2096
NspV TTCGAA 1 cut(s) 764
PaqCI CACCTGC 1 cut(s) 820
PctI GAATGC 1 cut(s) 1543
PdiI GCCGGC 1 cut(s) 1582
PdmI GAANNNNTTC 2 cut(s) 1249, 1828
PfeI GAWTC 8 cut(s) 71, 628, 646, 653, 904, 942, 976, 1730
PflFI GACNNNGTC 1 cut(s) 729
PflMI CCANNNNNTGG 1 cut(s) 1502
PkrI GCNGC 5 cut(s) 613, 739, 1176, 1324, 1966
PleI GAGTC 5 cut(s) 690, 738, 866, 1471, 2027
PpsI GAGTC 5 cut(s) 690, 738, 866, 1471, 2027
PpuMI RGGWCCY 2 cut(s) 1097, 1744
Psp124BI GAGCTC 1 cut(s) 1171
Psp5II RGGWCCY 2 cut(s) 1097, 1744
Psp6I CCWGG 4 cut(s) 484, 1177, 1783, 1978
PspGI CCWGG 4 cut(s) 484, 1177, 1783, 1978
PspN4I GGNNCC 8 cut(s) 801, 802, 970, 991, 1098, 1153, 1714, 1746
PspOMI GGGCCC 1 cut(s) 799
PspPI GGNCC 8 cut(s) 799, 800, 1036, 1070, 1097, 1434, 1744, 2026
PspPPI RGGWCCY 2 cut(s) 1097, 1744
PstI CTGCAG 3 cut(s) 1078, 1176, 1996
PstNI CAGNNNCTG 4 cut(s) 308, 1391, 1625, 1889
PsuI RGATCY 2 cut(s) 1621, 1663
PsyI GACNNNGTC 1 cut(s) 729
RsaI GTAC 8 cut(s) 10, 325, 838, 964, 1015, 1440, 1818, 1989
RsaNI GTAC 8 cut(s) 9, 324, 837, 963, 1014, 1439, 1817, 1988
RseI CAYNNNNRTG 2 cut(s) 750, 1160
SacI GAGCTC 1 cut(s) 1171
SacII CCGCGG 1 cut(s) 612
SaqAI TTAA 3 cut(s) 536, 1062, 1760
SatI GCNGC 5 cut(s) 612, 738, 1175, 1323, 1965
Sau96I GGNCC 8 cut(s) 799, 800, 1036, 1070, 1097, 1434, 1744, 2026
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 5 cut(s) 691, 739, 866, 1472, 2027
ScrFI CCNGG 4 cut(s) 486, 1179, 1785, 1980
SduI GDGCHC 7 cut(s) 42, 428, 574, 803, 1171, 1777, 2019
SfaNI GCATC 5 cut(s) 96, 824, 1042, 1297, 1568
SfcI CTRYAG 3 cut(s) 1074, 1172, 1992
Sfr303I CCGCGG 1 cut(s) 612
SfuI TTCGAA 1 cut(s) 764
SgrBI CCGCGG 1 cut(s) 612
SinI GGWCC 4 cut(s) 1070, 1097, 1434, 1744
SmiMI CAYNNNNRTG 2 cut(s) 750, 1160
SmlI CTYRAG 3 cut(s) 194, 1554, 1959
SmoI CTYRAG 3 cut(s) 194, 1554, 1959
Sse9I AATT 9 cut(s) 435, 708, 766, 982, 1794, 1828, 1927, 2005, 2053
SsiI CCGC 7 cut(s) 216, 414, 564, 609, 611, 1752, 2000
SspI AATATT 1 cut(s) 445
SspMI CTAG 6 cut(s) 290, 843, 1833, 1863, 1971, 2109
SstI GAGCTC 1 cut(s) 1171
StyD4I CCNGG 4 cut(s) 484, 1177, 1783, 1978
StyI CCWWGG 3 cut(s) 1161, 1220, 1415
TaaI ACNGT 4 cut(s) 1027, 1047, 1438, 2044
TaiI ACGT 1 cut(s) 2122
TaqI TCGA 5 cut(s) 337, 673, 764, 1279, 1293
TasI AATT 9 cut(s) 435, 708, 766, 982, 1794, 1828, 1927, 2005, 2053
TatI WGTACW 3 cut(s) 8, 323, 1816
TauI GCSGC 1 cut(s) 614
TfiI GAWTC 8 cut(s) 71, 628, 646, 653, 904, 942, 976, 1730
Tru1I TTAA 3 cut(s) 536, 1062, 1760
Tru9I TTAA 3 cut(s) 536, 1062, 1760
TscAI CASTG 5 cut(s) 463, 1032, 1062, 1270, 1819
TseFI GTSAC 3 cut(s) 916, 2044, 2096
TseI GCWGC 4 cut(s) 737, 1174, 1322, 1964
Tsp45I GTSAC 3 cut(s) 916, 2044, 2096
TspGWI ACGGA 4 cut(s) 771, 824, 935, 2063
TspRI CASTG 5 cut(s) 463, 1032, 1062, 1270, 1819
Tth111I GACNNNGTC 1 cut(s) 729
Van91I CCANNNNNTGG 1 cut(s) 1502
VpaK11BI GGWCC 4 cut(s) 1070, 1097, 1434, 1744
XagI CCTNNNNNAGG 1 cut(s) 1651
XapI RAATTY 6 cut(s) 435, 766, 1794, 1828, 2005, 2053
XmiI GTMKAC 1 cut(s) 1189
XmnI GAANNNNTTC 2 cut(s) 1249, 1828
XspI CTAG 6 cut(s) 290, 843, 1833, 1863, 1971, 2109
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.