MD15G1160800.v1.1

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007815
Physical Location & Seq
Reverse (-)
12025161 .. 12031966
6806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1160800.v1.1.491

Sequence Viewer

Length: 2172 bp
ATGGGAAGTAATGGTAGTGAACAGATTCTCATGGGTGAGCAGGTCGAAGGGTCTGAAGCCACTATTGAGATTAAAATAAAAACATTGGATTCACAAACCTATACTCTCAGGGTGGATAAACAGATGCCAGTTCCTGCTTTGAAAGAACAAGTTGCTTCTGTCACTGGTGTGCTATCAGAACAACAACGACTGATATGCCGAGGAAAAGTTCTAAAGGATGATCAGCTTCTCTCTGCCTATCATGTTGAAGATGGTCACACCTTACATTTGGTTGTGAGACAGCCTATTCCACTATCACCTGAAGGGTTACCAGATCATCAAGGAACTGATCCAGCTTCAAGTTCAAGCCGTCATGCTGGCCCTGGTGTTGTCATTGAAACTTTCAGCATGCCTGTTCAAGGGGATGGATTTGCACCTCAAATCAATCGGATAATCTCTGCTGTTCTCGGCTCTATAGGAATGCCAAATATTGCAGGTGGCAGTGAAGGGATTGAAGTCAGGGAACATGGGCCACAGAGGCCAGAAAGGACATTGGGTTTGGGTGGTTTGTTTGATTTCTCCCAATTTCAATCTGAACAAACTGCCGCAAGGGGTCCATCTGATAGATCAAATGGGACTTTTAGTCATTCAACATCATTTCCTTTGGGGGCCCTGCCACCTCTGGTAATTCCTGATTCTTTGACCACTTTAACCCAATATCTGAGTCATATGAGGCGTGAATTTGAGGCTATTGACAGAGATGCGGGAAGCAATCAAGCAGTTGCTACTCTTAGGGCTGAAGAAAGCTCCAATTCCTCATCAGGTTCAGGGATAAGGCAAGAGGGGCTCTCCACTCCTGCATCGTTGGCAGAAATCATGCGTTCTACTCGACAATTACTTGCTGAACAAGTTGGAGAATCCTTACTTCAATTTGCAAGTCAACTGGAGAATCAAGTGAATGTGACTGATTCTGCAGCACGGTTGAGCGCCCAAGCTAGTGCATCGAGAACTGGAGCTTTATTTCATAACCTAGGTGCCTTTTTACTTGAACTTGGTCGTACAACCATGACATTGCAAATGGGTCAAGCACCGTCTGACGCGGTGGTTAGTGCTGGACCTGCAGTTTTCATATCCCCTACTGGCCCTAATCCCATTATGGTTCAGCCTCTTCCCTTCCAATCTGGAACGAACTTTGGTGCCATCCCCATGGGAGCCATGCAGCCTGGTTCTGGTCTTGTTAGTGGACTCGGTACTGGTTTTCATCCAAGGCGTATTGATATACAAATACGAAGAGGTTCGTCGGCAACCACACCCGATGCCACTCGAGATGAACATGGGGAGACTCAGCAGCCTTCAGGGCAAAGAAACCCAGTAACAAGTTCTGGCAGTGAAAATCCTACTAATCAAGCCACTTCAAGGGTCTCAGGTGGTTCAGCTTTTGCCGGGGATCCGGCTGTAAGGATAATGCCAATCAGGACCATGGTTGCAGCAGTCCCTGCTTCCCTCAGTCGCCAACCATCAGATTCTTCTGGTATTTCGGGAGGGTTATACCATCCACTGCTTGGAAGGTTTCAACATGTAGCTTCTGGGAATGTGAATAGTGAACGAGGAACTCCAGCGTCCAGAGATCGTCACCCTGCCAATCTCCATACTGATCATCAGTCTTCTGAATCTGCAGCAGAACAGCAAAATGCTGCAGATCCTGCAAGAGATGGATCAGCTCCCAATGTAAGACGACCATCTATCTCACGAAGTGTCAGTATCAATATTTTATCAGCTGGTGGAACCCCAAACAGCCAAGAATCTGAGAGACAGGTCCCAGGTGGTATATTACAGTTTATAAGGACCCTCTTTCCGAATGGTGAAATTCATGTAGAAGACGGCAGTGCCGAGGGAGTGACTGCAGGTTCTGTACCAGATCAGCCAAGGCCATCTAGTGGTGGTGTGGCTGCACCGGAAGCAGAACCAAGGGCCAGCGATGAAGGAATATTTTTGTCTAATTTGCTTCATGAAATCATGCCATTCATATCTCAGGCCACAGGGGGAGAGCCAGGAAATTCTTCCGAGCACAGAATGGCCCAAGATTCTCCCACCAGGGCTGAGACCTCCGATGTTGGGTCATCCCGTCGGCGGAGTGATTCTGAAGAATCCGAACCAAATCTTCCTACTTCAAAACGCCAAAAGAAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001775 GO:0001822 GO:0002020 GO:0002253 GO:0002376 GO:0002429 GO:0002474 GO:0002682 GO:0002684 GO:0002757 GO:0002764 GO:0002768 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010604 GO:0010605 GO:0010941 GO:0012501 GO:0016020 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019882 GO:0019899 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030101 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0030544 GO:0031072 GO:0031323 GO:0031324 GO:0031325 GO:0031329 GO:0031330 GO:0031331 GO:0031593 GO:0031625 GO:0031647 GO:0031974 GO:0031981 GO:0031982 GO:0032268 GO:0032269 GO:0032270 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036506 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042802 GO:0042981 GO:0043021 GO:0043022 GO:0043066 GO:0043067 GO:0043069 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043230 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045321 GO:0045732 GO:0045861 GO:0045862 GO:0045995 GO:0046649 GO:0048002 GO:0048232 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051787 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0060548 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070059 GO:0070062 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0080134 GO:0080135 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1901800 GO:1903046 GO:1903050 GO:1903051 GO:1903052 GO:1903362 GO:1903363 GO:1903364 GO:1903561 GO:1904292 GO:1904294 GO:1904378 GO:1905897 GO:1905898 GO:1990381 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

724

Amino Acids

76.15

Weight (kDa)

5.51

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 22 - 92 2.3e-07 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 24 - 94 4.8e-20 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1820
AarI CACCTGC 1 cut(s) 464
Acc36I ACCTGC 4 cut(s) 31, 464, 1105, 1874
AccB1I GGYRCC 2 cut(s) 1013, 1175
AccB7I CCANNNNNTGG 2 cut(s) 1541, 1916
AccII CGCG 1 cut(s) 1079
AciI CCGC 4 cut(s) 585, 743, 1079, 2110
AclWI GGATC 5 cut(s) 323, 1421, 1434, 1673, 1702
AcsI RAATTY 3 cut(s) 719, 1845, 2035
AcuI CTGAAG 5 cut(s) 75, 321, 798, 1317, 2142
AdeI CACNNNGTG 1 cut(s) 1733
AfaI GTAC 3 cut(s) 1039, 1231, 1893
AfiI CCNNNNNNNGG 8 cut(s) 398, 1208, 1395, 1541, 1916, 2094, 2109, 2164
AflIII ACRYGT 1 cut(s) 1555
AhdI GACNNNNNGTC 1 cut(s) 621
AjnI CCWGG 5 cut(s) 361, 1201, 1798, 2029, 2072
AleI CACNNNNGTG 1 cut(s) 167
AluBI AGCT 9 cut(s) 226, 335, 786, 974, 995, 1415, 1562, 1700, 1757
AluI AGCT 9 cut(s) 226, 335, 786, 974, 995, 1415, 1562, 1700, 1757
Alw21I GWGCWC 1 cut(s) 2049
Alw26I GTCTC 5 cut(s) 271, 1313, 1405, 1783, 2075
AlwI GGATC 5 cut(s) 323, 1421, 1434, 1673, 1702
AlwNI CAGNNNCTG 4 cut(s) 134, 1475, 1682, 1889
Ama87I CYCGRG 1 cut(s) 1302
AoxI GGCC 9 cut(s) 358, 509, 518, 648, 1120, 1907, 1950, 2013, 2055
ApaI GGGCCC 1 cut(s) 652
ApeKI GCWGC 7 cut(s) 953, 1198, 1327, 1466, 1655, 1673, 1928
ApoI RAATTY 3 cut(s) 719, 1845, 2035
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
Asp700I GAANNNNTTC 3 cut(s) 24, 1273, 2038
AspA2I CCTAGG 1 cut(s) 1009
AspLEI GCGC 1 cut(s) 968
AsuC2I CCSGG 1 cut(s) 1423
AsuHPI GGTGA 4 cut(s) 47, 288, 1604, 1853
AvaI CYCGRG 1 cut(s) 1302
AvaII GGWCC 5 cut(s) 593, 1094, 1455, 1795, 1824
AvrII CCTAGG 1 cut(s) 1009
BaeGI GKGCMC 1 cut(s) 652
BamHI GGATCC 1 cut(s) 1426
BanI GGYRCC 2 cut(s) 1013, 1175
BanII GRGCYC 2 cut(s) 652, 828
BbsI GAAGAC 2 cut(s) 1635, 1863
Bbv12I GWGCWC 1 cut(s) 2049
BbvI GCAGC 7 cut(s) 965, 1210, 1339, 1478, 1660, 1667, 1915
BccI CCATC 9 cut(s) 245, 398, 604, 1187, 1504, 1539, 1685, 1726, 1918
BceAI ACGGC 2 cut(s) 333, 1876
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BciT130I CCWGG 5 cut(s) 363, 1203, 1800, 2031, 2074
BclI TGATCA 2 cut(s) 220, 1633
BcnI CCSGG 1 cut(s) 1423
BcoDI GTCTC 5 cut(s) 271, 1313, 1405, 1783, 2075
BfaI CTAG 3 cut(s) 975, 1010, 1914
BfmI CTRYAG 6 cut(s) 453, 951, 1098, 1653, 1674, 1881
BfoI RGCGCY 1 cut(s) 969
BfuAI ACCTGC 4 cut(s) 31, 464, 1105, 1874
BglI GCCNNNNNGGC 2 cut(s) 517, 1336
BisI GCNGC 8 cut(s) 585, 954, 1199, 1328, 1467, 1656, 1674, 1929
BlnI CCTAGG 1 cut(s) 1009
BlsI GCNGC 8 cut(s) 586, 955, 1200, 1329, 1468, 1657, 1675, 1930
Bme1390I CCNGG 6 cut(s) 363, 1203, 1423, 1800, 2031, 2074
Bme18I GGWCC 5 cut(s) 593, 1094, 1455, 1795, 1824
BmeRI GACNNNNNGTC 1 cut(s) 621
BmeT110I CYCGRG 1 cut(s) 1302
BmrFI CCNGG 6 cut(s) 363, 1203, 1423, 1800, 2031, 2074
BmrI ACTGGG 1 cut(s) 1343
BmsI GCATC 5 cut(s) 114, 730, 848, 989, 1285
BmuI ACTGGG 1 cut(s) 1343
BpiI GAAGAC 2 cut(s) 1635, 1863
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpmI CTGGAG 3 cut(s) 944, 1011, 1578
BpuMI CCSGG 1 cut(s) 1423
BsaI GGTCTC 2 cut(s) 1405, 2075
BsaWI WCCGGW 1 cut(s) 1933
Bsc4I CCNNNNNNNGG 8 cut(s) 398, 1208, 1395, 1541, 1916, 2094, 2109, 2164
Bse1I ACTGG 7 cut(s) 128, 169, 927, 994, 1123, 1237, 1349
Bse3DI GCAATG 1 cut(s) 1049
BseBI CCWGG 5 cut(s) 363, 1203, 1800, 2031, 2074
BseGI GGATG 6 cut(s) 223, 409, 1179, 1240, 1531, 2099
BseLI CCNNNNNNNGG 8 cut(s) 398, 1208, 1395, 1541, 1916, 2094, 2109, 2164
BseMI GCAATG 1 cut(s) 1049
BseMII CTCAG 8 cut(s) 121, 692, 1337, 1416, 1498, 1776, 2024, 2070
BseNI ACTGG 7 cut(s) 128, 169, 927, 994, 1123, 1237, 1349
BseSI GKGCMC 1 cut(s) 652
BseXI GCAGC 7 cut(s) 965, 1210, 1339, 1478, 1660, 1667, 1915
BsgI GTGCAG 1 cut(s) 1914
Bsh1236I CGCG 1 cut(s) 1079
BshFI GGCC 9 cut(s) 360, 511, 520, 650, 1122, 1909, 1952, 2015, 2057
BshNI GGYRCC 2 cut(s) 1013, 1175
BsiHKAI GWGCWC 1 cut(s) 2049
BsiHKCI CYCGRG 1 cut(s) 1302
BsiSI CCGG 3 cut(s) 1422, 1430, 1934
BslFI GGGAC 3 cut(s) 628, 1457, 1781
BslI CCNNNNNNNGG 8 cut(s) 398, 1208, 1395, 1541, 1916, 2094, 2109, 2164
BsmAI GTCTC 5 cut(s) 271, 1313, 1405, 1783, 2075
BsmFI GGGAC 3 cut(s) 628, 1457, 1781
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 9 cut(s) 360, 511, 520, 650, 1122, 1909, 1952, 2015, 2057
Bso31I GGTCTC 2 cut(s) 1405, 2075
BsoBI CYCGRG 1 cut(s) 1302
Bsp120I GGGCCC 1 cut(s) 648
Bsp1286I GDGCHC 3 cut(s) 652, 828, 2049
Bsp19I CCATGG 2 cut(s) 1185, 1458
BspACI CCGC 4 cut(s) 585, 743, 1079, 2110
BspANI GGCC 9 cut(s) 360, 511, 520, 650, 1122, 1909, 1952, 2015, 2057
BspCNI CTCAG 8 cut(s) 120, 693, 1336, 1415, 1497, 1777, 2023, 2071
BspFNI CGCG 1 cut(s) 1079
BspHI TCATGA 1 cut(s) 1987
BspMAI CTGCAG 5 cut(s) 955, 1102, 1657, 1678, 1885
BspMI ACCTGC 4 cut(s) 31, 464, 1105, 1874
BspPI GGATC 5 cut(s) 323, 1421, 1434, 1673, 1702
BspT107I GGYRCC 2 cut(s) 1013, 1175
BspTNI GGTCTC 2 cut(s) 1405, 2075
BsrDI GCAATG 1 cut(s) 1049
BsrI ACTGG 7 cut(s) 128, 169, 927, 994, 1123, 1237, 1349
BssT1I CCWWGG 6 cut(s) 1009, 1185, 1244, 1458, 1904, 1946
Bst2UI CCWGG 5 cut(s) 363, 1203, 1800, 2031, 2074
Bst4CI ACNGT 3 cut(s) 960, 1071, 1815
Bst6I CTCTTC 2 cut(s) 1152, 1264
BstAPI GCANNNNNTGC 2 cut(s) 1475, 1682
BstC8I GCNNGC 3 cut(s) 358, 389, 1954
BstDEI CTNAG 9 cut(s) 107, 701, 770, 1323, 1402, 1484, 1785, 2010, 2079
BstDSI CCRYGG 2 cut(s) 1185, 1458
BstEII GGTNACC 1 cut(s) 306
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 6 cut(s) 223, 409, 1179, 1240, 1531, 2099
BstFNI CGCG 1 cut(s) 1079
BstH2I RGCGCY 1 cut(s) 969
BstHHI GCGC 1 cut(s) 968
BstMAI GTCTC 5 cut(s) 271, 1313, 1405, 1783, 2075
BstMWI GCNNNNNNNGC 8 cut(s) 517, 823, 845, 1097, 1336, 1475, 1682, 1937
BstNI CCWGG 5 cut(s) 363, 1203, 1800, 2031, 2074
BstNSI RCATGY 2 cut(s) 391, 1559
BstPI GGTNACC 1 cut(s) 306
BstSCI CCNGG 6 cut(s) 361, 1201, 1421, 1798, 2029, 2072
BstSFI CTRYAG 6 cut(s) 453, 951, 1098, 1653, 1674, 1881
BstSLI GKGCMC 1 cut(s) 652
BstUI CGCG 1 cut(s) 1079
BstV1I GCAGC 7 cut(s) 965, 1210, 1339, 1478, 1660, 1667, 1915
BstV2I GAAGAC 2 cut(s) 1635, 1863
BstX2I RGATCY 2 cut(s) 1426, 1678
BstXI CCANNNNNNTGG 1 cut(s) 1186
BstYI RGATCY 2 cut(s) 1426, 1678
BsuRI GGCC 9 cut(s) 360, 511, 520, 650, 1122, 1909, 1952, 2015, 2057
BtgI CCRYGG 2 cut(s) 1185, 1458
BtgZI GCGATG 1 cut(s) 1971
BtsCI GGATG 6 cut(s) 223, 409, 1179, 1240, 1531, 2099
BtsI GCAGTG 4 cut(s) 487, 1372, 1535, 1870
BtsIMutI CAGTG 5 cut(s) 162, 487, 1372, 1535, 1870
BveI ACCTGC 4 cut(s) 31, 464, 1105, 1874
Cac8I GCNNGC 3 cut(s) 358, 389, 1954
CaiI CAGNNNCTG 4 cut(s) 134, 1475, 1682, 1889
CciI TCATGA 1 cut(s) 1987
CfoI GCGC 1 cut(s) 968
CseI GACGC 2 cut(s) 1085, 1587
Csp6I GTAC 3 cut(s) 1038, 1230, 1892
CviQI GTAC 3 cut(s) 1038, 1230, 1892
DdeI CTNAG 9 cut(s) 107, 701, 770, 1323, 1402, 1484, 1785, 2010, 2079
DraIII CACNNNGTG 1 cut(s) 1733
DriI GACNNNNNGTC 1 cut(s) 621
Eam1104I CTCTTC 2 cut(s) 1152, 1264
Eam1105I GACNNNNNGTC 1 cut(s) 621
EarI CTCTTC 2 cut(s) 1152, 1264
EciI GGCGGA 1 cut(s) 2125
Eco130I CCWWGG 6 cut(s) 1009, 1185, 1244, 1458, 1904, 1946
Eco24I GRGCYC 2 cut(s) 652, 828
Eco31I GGTCTC 2 cut(s) 1405, 2075
Eco47I GGWCC 5 cut(s) 593, 1094, 1455, 1795, 1824
Eco57I CTGAAG 5 cut(s) 75, 321, 798, 1317, 2142
Eco88I CYCGRG 1 cut(s) 1302
Eco91I GGTNACC 1 cut(s) 306
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoO109I RGGNCCY 4 cut(s) 648, 649, 1795, 1824
EcoO65I GGTNACC 1 cut(s) 306
EcoRII CCWGG 5 cut(s) 361, 1201, 1798, 2029, 2072
EcoT14I CCWWGG 6 cut(s) 1009, 1185, 1244, 1458, 1904, 1946
EcoT38I GRGCYC 2 cut(s) 652, 828
ErhI CCWWGG 6 cut(s) 1009, 1185, 1244, 1458, 1904, 1946
FaqI GGGAC 3 cut(s) 628, 1457, 1781
FauI CCCGC 1 cut(s) 736
FauNDI CATATG 1 cut(s) 708
FbaI TGATCA 2 cut(s) 220, 1633
Fnu4HI GCNGC 8 cut(s) 585, 954, 1199, 1328, 1467, 1656, 1674, 1929
FokI GGATG 6 cut(s) 230, 416, 1166, 1227, 1518, 2086
FriOI GRGCYC 2 cut(s) 652, 828
Fsp4HI GCNGC 8 cut(s) 585, 954, 1199, 1328, 1467, 1656, 1674, 1929
FspBI CTAG 3 cut(s) 975, 1010, 1914
GlaI GCGC 1 cut(s) 967
GluI GCNGC 8 cut(s) 585, 954, 1199, 1328, 1467, 1656, 1674, 1929
GsuI CTGGAG 3 cut(s) 944, 1011, 1578
HaeII RGCGCY 1 cut(s) 969
HaeIII GGCC 9 cut(s) 360, 511, 520, 650, 1122, 1909, 1952, 2015, 2057
HapII CCGG 3 cut(s) 1422, 1430, 1934
HgaI GACGC 2 cut(s) 1085, 1587
HhaI GCGC 1 cut(s) 968
Hin6I GCGC 1 cut(s) 966
HinP1I GCGC 1 cut(s) 966
HincII GTYRAC 1 cut(s) 920
HindII GTYRAC 1 cut(s) 920
HpaII CCGG 3 cut(s) 1422, 1430, 1934
HphI GGTGA 4 cut(s) 47, 288, 1604, 1853
Hpy166II GTNNAC 4 cut(s) 20, 920, 1223, 1583
Hpy188III TCNNGA 9 cut(s) 671, 984, 1161, 1304, 1453, 1518, 1602, 1728, 1988
Hpy8I GTNNAC 4 cut(s) 20, 920, 1223, 1583
Hpy99I CGWCG 2 cut(s) 1282, 2109
HpyAV CCTTC 8 cut(s) 41, 296, 479, 1162, 1341, 1539, 1955, 2158
HpyCH4III ACNGT 3 cut(s) 960, 1071, 1815
HpyF10VI GCNNNNNNNGC 8 cut(s) 517, 823, 845, 1097, 1336, 1475, 1682, 1937
HpyF3I CTNAG 9 cut(s) 107, 701, 770, 1323, 1402, 1484, 1785, 2010, 2079
HspAI GCGC 1 cut(s) 966
Ksp22I TGATCA 2 cut(s) 220, 1633
LmnI GCTCC 4 cut(s) 791, 992, 1190, 1705
Lsp1109I GCAGC 7 cut(s) 965, 1210, 1339, 1478, 1660, 1667, 1915
LweI GCATC 5 cut(s) 114, 730, 848, 989, 1285
MaeI CTAG 3 cut(s) 975, 1010, 1914
MaeIII GTNAC 7 cut(s) 160, 254, 306, 940, 1351, 1610, 1876
MflI RGATCY 2 cut(s) 1426, 1678
MhlI GDGCHC 3 cut(s) 652, 828, 2049
MluCI AATT 9 cut(s) 563, 666, 719, 790, 872, 908, 1845, 1978, 2035
MlyI GAGTC 3 cut(s) 712, 1218, 1315
MmeI TCCRAC 1 cut(s) 871
MroXI GAANNNNTTC 3 cut(s) 24, 1273, 2038
MseI TTAA 2 cut(s) 72, 689
MslI CAYNNNNRTG 2 cut(s) 167, 1184
MspA1I CMGCKG 1 cut(s) 1757
MspI CCGG 3 cut(s) 1422, 1430, 1934
MspR9I CCNGG 6 cut(s) 363, 1203, 1423, 1800, 2031, 2074
Mva1269I GAATGC 1 cut(s) 465
MvaI CCWGG 5 cut(s) 363, 1203, 1800, 2031, 2074
MvnI CGCG 1 cut(s) 1079
MwoI GCNNNNNNNGC 8 cut(s) 517, 823, 845, 1097, 1336, 1475, 1682, 1937
NciI CCSGG 1 cut(s) 1423
NcoI CCATGG 2 cut(s) 1185, 1458
NdeI CATATG 1 cut(s) 708
NmeAIII GCCGAG 3 cut(s) 224, 426, 1894
NmuCI GTSAC 5 cut(s) 160, 254, 940, 1610, 1876
NspI RCATGY 2 cut(s) 391, 1559
OliI CACNNNNGTG 1 cut(s) 167
PaeI GCATGC 1 cut(s) 391
PaeR7I CTCGAG 1 cut(s) 1302
PagI TCATGA 1 cut(s) 1987
PaqCI CACCTGC 1 cut(s) 464
PciI ACATGT 1 cut(s) 1555
PcsI WCGNNNNNNNCGW 1 cut(s) 1866
PctI GAATGC 1 cut(s) 465
PdmI GAANNNNTTC 3 cut(s) 24, 1273, 2038
PflMI CCANNNNNTGG 2 cut(s) 1541, 1916
PkrI GCNGC 8 cut(s) 586, 955, 1200, 1329, 1468, 1657, 1675, 1930
PleI GAGTC 3 cut(s) 711, 1218, 1315
PpsI GAGTC 3 cut(s) 711, 1218, 1315
PpuMI RGGWCCY 2 cut(s) 1795, 1824
PscI ACATGT 1 cut(s) 1555
PsiI TTATAA 1 cut(s) 1820
Psp5II RGGWCCY 2 cut(s) 1795, 1824
Psp6I CCWGG 5 cut(s) 361, 1201, 1798, 2029, 2072
PspEI GGTNACC 1 cut(s) 306
PspGI CCWGG 5 cut(s) 361, 1201, 1798, 2029, 2072
PspOMI GGGCCC 1 cut(s) 648
PspPPI RGGWCCY 2 cut(s) 1795, 1824
PstI CTGCAG 5 cut(s) 955, 1102, 1657, 1678, 1885
PstNI CAGNNNCTG 4 cut(s) 134, 1475, 1682, 1889
PsuI RGATCY 2 cut(s) 1426, 1678
PvuII CAGCTG 1 cut(s) 1757
RsaI GTAC 3 cut(s) 1039, 1231, 1893
RsaNI GTAC 3 cut(s) 1038, 1230, 1892
RseI CAYNNNNRTG 2 cut(s) 167, 1184
SaqAI TTAA 2 cut(s) 72, 689
SatI GCNGC 8 cut(s) 585, 954, 1199, 1328, 1467, 1656, 1674, 1929
SchI GAGTC 3 cut(s) 712, 1218, 1315
ScrFI CCNGG 6 cut(s) 363, 1203, 1423, 1800, 2031, 2074
SduI GDGCHC 3 cut(s) 652, 828, 2049
SfaNI GCATC 5 cut(s) 114, 730, 848, 989, 1285
SfcI CTRYAG 6 cut(s) 453, 951, 1098, 1653, 1674, 1881
SfiI GGCCNNNNNGGCC 1 cut(s) 517
Sfr274I CTCGAG 1 cut(s) 1302
SinI GGWCC 5 cut(s) 593, 1094, 1455, 1795, 1824
SlaI CTCGAG 1 cut(s) 1302
SmiMI CAYNNNNRTG 2 cut(s) 167, 1184
SmlI CTYRAG 1 cut(s) 1302
SmoI CTYRAG 1 cut(s) 1302
SphI GCATGC 1 cut(s) 391
Sse9I AATT 9 cut(s) 563, 666, 719, 790, 872, 908, 1845, 1978, 2035
SsiI CCGC 4 cut(s) 585, 743, 1079, 2110
SspI AATATT 3 cut(s) 469, 1747, 1968
SspMI CTAG 3 cut(s) 975, 1010, 1914
StyD4I CCNGG 6 cut(s) 361, 1201, 1421, 1798, 2029, 2072
StyI CCWWGG 6 cut(s) 1009, 1185, 1244, 1458, 1904, 1946
TaaI ACNGT 3 cut(s) 960, 1071, 1815
TaqI TCGA 4 cut(s) 45, 868, 983, 1303
TasI AATT 9 cut(s) 563, 666, 719, 790, 872, 908, 1845, 1978, 2035
TauI GCSGC 1 cut(s) 587
Tru1I TTAA 2 cut(s) 72, 689
Tru9I TTAA 2 cut(s) 72, 689
TscAI CASTG 5 cut(s) 169, 487, 1372, 1542, 1870
TseFI GTSAC 5 cut(s) 160, 254, 940, 1610, 1876
TseI GCWGC 7 cut(s) 953, 1198, 1327, 1466, 1655, 1673, 1928
Tsp45I GTSAC 5 cut(s) 160, 254, 940, 1610, 1876
TspDTI ATGAA 9 cut(s) 992, 1096, 1229, 1323, 1838, 1974, 1976, 1993, 2004
TspRI CASTG 5 cut(s) 169, 487, 1372, 1542, 1870
Van91I CCANNNNNTGG 2 cut(s) 1541, 1916
VpaK11BI GGWCC 5 cut(s) 593, 1094, 1455, 1795, 1824
XagI CCTNNNNNAGG 1 cut(s) 396
XapI RAATTY 3 cut(s) 719, 1845, 2035
XceI RCATGY 2 cut(s) 391, 1559
XcmI CCANNNNNNNNNTGG 1 cut(s) 1538
XhoI CTCGAG 1 cut(s) 1302
XmaJI CCTAGG 1 cut(s) 1009
XmnI GAANNNNTTC 3 cut(s) 24, 1273, 2038
XspI CTAG 3 cut(s) 975, 1010, 1914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.