Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010738

Physical Location & Seq

Genomic Coordinates Reverse (-)

8803829 .. 8807783

Gene Structure 3955 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G25370.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGACGGAGCAATTGCTGCTTCATGGAACTCTTGAAGTGAAGATTTATAGGATCGATAAGTTGCATCAACGTTCAAGATTCAATTTATGCGGCAAGGGGAACAAGGAGCCAACAGGTAAAAAGACTCAATCTCAAATCAAAAGACTAACGGACTCATGCACAAGTTTGTTTGGAGGACATCTATACGCAACTATTGACCTAGACAGGTCAAGAGTGGCTAGAACCATGATGAGACGTCATCCTAAATGGTTACAATCCTTCCACGTCTACACCGCACATTCAATCTCTAAAATCATATTCACAGTTAAAGAAGATGAACCGGTCAGTGCTAGTTTGATAGGGCGAGCTTATTTACCTGTAACAGAAGTCATCACCGGACAACCTATAGACCGATGGCTCGATATCTTAGACGAGAACAGGAGACCAATCCAAGGAGGTTCCAAACTCCATGTACGTGTGAAGTTCACTCATGTGACACAGGACGTGAATTGGAACAAAGGAATAATATTACCTTCCTTCAATGGAGTTCCTAACGCTTATTTCAACCAAAGAGAAGGTTGCAAAGTTACACTATACCAAGACGCTCACGTTCTTAACGAATACCCTGATGTTACCCTCACGGGAGGACAAGTTATATACAAGCATCATCGGTGTTGGGAAGAGATTTTCGATGCGATATGGGAAGCAAAACATTTGATATACATCGCTGGTTGGTCCGTAAACACCGACGTGACATTAGTTAGAGATCCTAAACGGACGAGACCGGGAGGGGACCTCAAGTTAGGGGAATTGCTGAAGAAGAAAGCAGAAGAAAACGTGACCGTGCTGATGCTTGTGTGGGACGATAGAACGTCTCACGAAGTTTTTAAGAGAGACGGTTTGATGATGACTCATGATCAAGAAACTTACGATTACTTCAAGAACACGAAAGTGCGTTGCGTTCTCTGTCCGCGGAATCCGGATAATGGTGATAGCATTGTGCAAGGGTTCGAGGTCGCTACTATGTTTACTCATCATCAAAAAACGATTGTGGTGGACAGTGAAGTGGACGGGTCATTAACCAAGAGAAGGATAGTGAGTTTTCTTGGAGGTATTGATCTTTGTGATGGGAGATACGACACTGTAGAGCATCCTTTGTTTGGTACTTTGAATAGTGTTCATGCTAATGACTTTCACCAGCCGAACTTTGATGGTGCATCGATTAAAAAGGGCGGTCCACGTGAACCGTGGCACGATATTCACTGCAAGCTGGATGGTCCTGCGGCGTGGGATGTTTTATACAATTTTGAGCAGAGGTGGATGAAACAAGGTAGTGGTAGAAGGTACCTTATATCGATGGCACAGCTCGCAGAGATCACGGTCCCGCCACTTCCGATTGTACAACCAGACAATGAAGAAGGTTGGACAGTTCAAGTCTTCCGGTCGATAGATGATGGTGCAGTTGAAGGGTTCCCAGAGGATCCACGTGAAGCTGCAAGCATAGGACTTATAAGCGGAAAAGATAACGTGATCGAAAGAAGCATTCAAGACGCTTACGTTAATGCCATAAGGAGAGCCAAAAACTTCATCTACATTGAGAACCAATATTTTCTAGGAAGCTCCTTTGGATGGAACTCTAGAGACATAAATTTAAATGAGATCAATGCACTACAACTCATTCCTAAAGAGATTTCTCTAAAAATCGTGAGTAAAATCGAGGCAGGCGAGCGGTTTTCGGTGTATATAGTGATTCCGTTATGGCCTGAAGGTAAACCTGGTTCCGCATCGGTTCAAGCAATACTAGACTGGCAAAGGAGAACAATGGAGATGATGTATACTGATATAATCATTGCACTACGTAAAAAAGGTTTAGATGCAAACCCTAGAGACTACCTAACGTTTTTCTGCCTCGGGAACCGGGAAAAGGGCAAGGTGGGTGAATACTTGCCTCCGGAAAAACCGGAGGCTAATTCTGATTACGCAAGAGCTCAAGAATCTCGTCGGTTCATGATCTATGTCCACTCTAAAATGATGATTGTTGATGATGAGTACATAATAATAGGATCAGCCAATATAAACCAAAGGTCAATGGATGGAGGTCGAGACACAGAGATCGCCATGGGAGCATATCAACCTAGCCATCTCCTATCTACTAATAATATGAGGCCGGTTGGCCAAATATTTAGCTTCAGGATATCACTATGGCTTGAACACCTACGAGTAACAACCAATGCATTTCAGTGCCCCGAAAGCGAAGAGTGTATAAGAATGGTTAATGCAACGGCAGACGAGCTATGGGGACTATACTCAGCACAAGAGTATCCTCGAAATGACGATTTACCGGGGCATTTGCTTAGTTATCCGATTAGCATTGGTAGCAATGGGGAAGTAACAAATCTTGCTGGGACTGAATTCTTTCCTGATACTAACGCAAAGGTTGTAGGCGAAAAATCTAATTATCTTCCTCCAATCCTTACTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0006629 GO:0006643 GO:0006950 GO:0006970 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010035 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0022622 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042578 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0046466 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0071704 GO:0097305 GO:0099402 GO:1901575 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

820

Amino Acids

93.36

Weight (kDa)

6.36

Isoelectric Point (pI)

38.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc_2	PF13091	663 - 698	3.1e-06	PLD-like domain
PLDc	PF00614	664 - 689	3.5e-08	Phospholipase D Active site motif
PLD_C	PF12357	737 - 810	6.6e-25	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25370 AT5G25370 AT5G25370 AT5G25370
fragaria_vesca	FvH4_1g03170
malus_domestica	MD02G1030700.v1.1 MD15G1172300.v1.1
prunus_persica	Prupe.7G243800_v2.0.a1
pyrus_communis	pycom02g02560 pycom15g15370
rosa_chinensis	RchiOBHm_Chr2g0088301
rosa_laevigata	RLG00000015940
rosa_multiflora	Rmu_sc0004235.1_g000036
rosa_roxburghii	Rroxscaffold_2G00152760
rosa_rugosa	Rorug01G0482700
rosa_samantha	Rh2AG037800 Rh2BG037000 Rh2CG038100 Rh2DG037500
rosa_wichuraiana	Rw2G002980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1490
AatII	GACGTC	1 cut(s)	238
Acc65I	GGTACC	1 cut(s)	1323
AccB1I	GGYRCC	1 cut(s)	1323
AccBSI	CCGCTC	1 cut(s)	1708
AccI	GTMKAC	2 cut(s)	267, 1814
AccII	CGCG	1 cut(s)	952
AccIII	TCCGGA	2 cut(s)	958, 1930
AclI	AACGTT	2 cut(s)	70, 1877
AclWI	GGATC	5 cut(s)	59, 740, 1454, 1467, 2050
AcoI	YGGCCR	1 cut(s)	2152
AcsI	RAATTY	2 cut(s)	1627, 2390
AcuI	CTGAAG	3 cut(s)	815, 1764, 2152
AcvI	CACGTG	2 cut(s)	1220, 1466
AcyI	GRCGYC	1 cut(s)	235
AfaI	GTAC	5 cut(s)	453, 1144, 1325, 1380, 2030
AfiI	CCNNNNNNNGG	6 cut(s)	431, 965, 1068, 1139, 1608, 1903
AflIII	ACRYGT	1 cut(s)	454
AgeI	ACCGGT	1 cut(s)	319
AjiI	CACGTC	3 cut(s)	265, 484, 730
AjnI	CCWGG	1 cut(s)	1753
AjuI	GAANNNNNNNTTGG	1 cut(s)	2440
AleI	CACNNNNGTG	3 cut(s)	470, 728, 929
AluBI	AGCT	8 cut(s)	347, 1249, 1345, 1472, 1599, 1967, 2166, 2272
AluI	AGCT	8 cut(s)	347, 1249, 1345, 1472, 1599, 1967, 2166, 2272
Alw21I	GWGCWC	1 cut(s)	1969
Alw26I	GTCTC	8 cut(s)	226, 415, 754, 858, 867, 1614, 1860, 2076
AlwI	GGATC	5 cut(s)	59, 740, 1454, 1467, 2050
Ama87I	CYCGRG	1 cut(s)	1889
Aor13HI	TCCGGA	2 cut(s)	958, 1930
AoxI	GGCC	3 cut(s)	1739, 2144, 2152
ApeKI	GCWGC	2 cut(s)	16, 1472
ApoI	RAATTY	2 cut(s)	1627, 2390
AsiGI	ACCGGT	1 cut(s)	319
Asp700I	GAANNNNTTC	1 cut(s)	2394
Asp718I	GGTACC	1 cut(s)	1323
AspS9I	GGNCC	5 cut(s)	714, 772, 1214, 1256, 1360
AsuC2I	CCSGG	3 cut(s)	765, 1898, 2322
AsuHPI	GGTGA	4 cut(s)	364, 980, 1166, 1928
AvaI	CYCGRG	1 cut(s)	1889
AvaII	GGWCC	5 cut(s)	714, 772, 1214, 1256, 1360
BaeGI	GKGCMC	1 cut(s)	2225
BaeI	ACNNNNGTAYC	2 cut(s)	2282, 2315
BalI	TGGCCA	1 cut(s)	2154
BamHI	GGATCC	1 cut(s)	1459
BanI	GGYRCC	1 cut(s)	1323
BanII	GRGCYC	1 cut(s)	1969
BbrPI	CACGTG	2 cut(s)	1220, 1466
BbsI	GAAGAC	1 cut(s)	1408
Bbv12I	GWGCWC	1 cut(s)	1969
BbvI	GCAGC	2 cut(s)	3, 1459
BccI	CCATC	9 cut(s)	387, 1100, 1184, 1247, 1330, 1427, 1602, 2066, 2127
BceAI	ACGGC	1 cut(s)	2277
BciT130I	CCWGG	1 cut(s)	1755
BciVI	GTATCC	1 cut(s)	2310
BclI	TGATCA	1 cut(s)	895
BcnI	CCSGG	3 cut(s)	765, 1898, 2322
BcoDI	GTCTC	8 cut(s)	226, 415, 754, 858, 867, 1614, 1860, 2076
BfaI	CTAG	8 cut(s)	200, 219, 330, 1592, 1617, 1781, 1863, 2115
BfmI	CTRYAG	2 cut(s)	384, 1122
BfuI	GTATCC	1 cut(s)	2310
BisI	GCNGC	4 cut(s)	17, 91, 1263, 1473
BlsI	GCNGC	4 cut(s)	18, 92, 1264, 1474
Bme1390I	CCNGG	4 cut(s)	765, 1755, 1898, 2322
Bme18I	GGWCC	5 cut(s)	714, 772, 1214, 1256, 1360
BmeT110I	CYCGRG	1 cut(s)	1889
BmgBI	CACGTC	3 cut(s)	265, 484, 730
BmgT120I	GGNCC	5 cut(s)	714, 772, 1214, 1256, 1360
BmiI	GGNNCC	9 cut(s)	108, 439, 773, 1325, 1362, 1451, 1461, 1759, 1895
BmrFI	CCNGG	4 cut(s)	765, 1755, 1898, 2322
BmsI	GCATC	8 cut(s)	73, 652, 661, 819, 1138, 1205, 1772, 1843
BpiI	GAAGAC	1 cut(s)	1408
BplI	GAGNNNNNCTC	2 cut(s)	759, 791
BpuEI	CTTGAG	2 cut(s)	761, 1953
BpuMI	CCSGG	3 cut(s)	765, 1898, 2322
Bsa29I	ATCGAT	3 cut(s)	54, 1199, 1334
BsaAI	YACGTR	4 cut(s)	455, 1220, 1466, 1838
BsaBI	GATNNNNATC	1 cut(s)	701
BsaHI	GRCGYC	1 cut(s)	235
BsaI	GGTCTC	2 cut(s)	415, 754
BsaJI	CCNNGG	6 cut(s)	430, 950, 1226, 1888, 2097, 2321
BsaWI	WCCGGW	6 cut(s)	319, 374, 958, 1419, 1930, 1939
BsaXI	ACNNNNNCTCC	4 cut(s)	615, 645, 1796, 1826
Bsc4I	CCNNNNNNNGG	6 cut(s)	431, 965, 1068, 1139, 1608, 1903
Bse118I	RCCGGY	2 cut(s)	319, 2146
Bse1I	ACTGG	1 cut(s)	1790
Bse3DI	GCAATG	2 cut(s)	1827, 2365
Bse8I	GATNNNNATC	1 cut(s)	701
BseAI	TCCGGA	2 cut(s)	958, 1930
BseBI	CCWGG	1 cut(s)	1755
BseCI	ATCGAT	3 cut(s)	54, 1199, 1334
BseDI	CCNNGG	6 cut(s)	430, 950, 1226, 1888, 2097, 2321
BseGI	GGATG	7 cut(s)	238, 1129, 1258, 1276, 1305, 1613, 2077
BseJI	GATNNNNATC	1 cut(s)	701
BseLI	CCNNNNNNNGG	6 cut(s)	431, 965, 1068, 1139, 1608, 1903
BseMI	GCAATG	2 cut(s)	1827, 2365
BseMII	CTCAG	1 cut(s)	2301
BseNI	ACTGG	1 cut(s)	1790
BseSI	GKGCMC	1 cut(s)	2225
BseXI	GCAGC	2 cut(s)	3, 1459
BseYI	CCCAGC	1 cut(s)	2381
BsgI	GTGCAG	1 cut(s)	1458
Bsh1236I	CGCG	1 cut(s)	952
Bsh1285I	CGRYCG	1 cut(s)	1424
BshFI	GGCC	3 cut(s)	1741, 2146, 2154
BshNI	GGYRCC	1 cut(s)	1323
BshTI	ACCGGT	1 cut(s)	319
BshVI	ATCGAT	3 cut(s)	54, 1199, 1334
BsiEI	CGRYCG	1 cut(s)	1424
BsiHKAI	GWGCWC	1 cut(s)	1969
BsiHKCI	CYCGRG	1 cut(s)	1889
BslFI	GGGAC	5 cut(s)	785, 854, 1346, 2292, 2398
BslI	CCNNNNNNNGG	6 cut(s)	431, 965, 1068, 1139, 1608, 1903
BsmAI	GTCTC	8 cut(s)	226, 415, 754, 858, 867, 1614, 1860, 2076
BsmBI	CGTCTC	3 cut(s)	226, 858, 867
BsmFI	GGGAC	5 cut(s)	785, 854, 1346, 2292, 2398
BsmI	GAATGC	1 cut(s)	1521
BsnI	GGCC	3 cut(s)	1741, 2146, 2154
Bso31I	GGTCTC	2 cut(s)	415, 754
BsoBI	CYCGRG	1 cut(s)	1889
Bsp1286I	GDGCHC	2 cut(s)	1969, 2225
Bsp13I	TCCGGA	2 cut(s)	958, 1930
Bsp1407I	TGTACA	1 cut(s)	1378
Bsp19I	CCATGG	1 cut(s)	2097
BspANI	GGCC	3 cut(s)	1741, 2146, 2154
BspCNI	CTCAG	1 cut(s)	2300
BspDI	ATCGAT	3 cut(s)	54, 1199, 1334
BspEI	TCCGGA	2 cut(s)	958, 1930
BspFNI	CGCG	1 cut(s)	952
BspHI	TCATGA	2 cut(s)	892, 1986
BspLI	GGNNCC	9 cut(s)	108, 439, 773, 1325, 1362, 1451, 1461, 1759, 1895
BspPI	GGATC	5 cut(s)	59, 740, 1454, 1467, 2050
BspT107I	GGYRCC	1 cut(s)	1323
BspTNI	GGTCTC	2 cut(s)	415, 754
BsrBI	CCGCTC	1 cut(s)	1708
BsrDI	GCAATG	2 cut(s)	1827, 2365
BsrFI	RCCGGY	2 cut(s)	319, 2146
BsrGI	TGTACA	1 cut(s)	1378
BsrI	ACTGG	1 cut(s)	1790
BssAI	RCCGGY	2 cut(s)	319, 2146
BssECI	CCNNGG	6 cut(s)	430, 950, 1226, 1888, 2097, 2321
BssNAI	GTATAC	1 cut(s)	1815
BssNI	GRCGYC	1 cut(s)	235
BssT1I	CCWWGG	2 cut(s)	430, 2097
Bst1107I	GTATAC	1 cut(s)	1815
Bst2UI	CCWGG	1 cut(s)	1755
Bst4CI	ACNGT	8 cut(s)	304, 823, 878, 1040, 1123, 1227, 1360, 1408
Bst6I	CTCTTC	2 cut(s)	654, 2229
BstACI	GRCGYC	1 cut(s)	235
BstAPI	GCANNNNNTGC	1 cut(s)	16
BstAUI	TGTACA	1 cut(s)	1378
BstBAI	YACGTR	4 cut(s)	455, 1220, 1466, 1838
BstC8I	GCNNGC	6 cut(s)	345, 1247, 1347, 1477, 1702, 1706
BstDEI	CTNAG	4 cut(s)	406, 2287, 2333, 2460
BstDSI	CCRYGG	3 cut(s)	950, 1226, 2097
BstF5I	GGATG	7 cut(s)	238, 1129, 1258, 1276, 1305, 1613, 2077
BstFNI	CGCG	1 cut(s)	952
BstMAI	GTCTC	8 cut(s)	226, 415, 754, 858, 867, 1614, 1860, 2076
BstMCI	CGRYCG	1 cut(s)	1424
BstMWI	GCNNNNNNNGC	5 cut(s)	16, 1346, 2102, 2229, 2355
BstNI	CCWGG	1 cut(s)	1755
BstSCI	CCNGG	4 cut(s)	763, 1753, 1896, 2320
BstSFI	CTRYAG	2 cut(s)	384, 1122
BstSLI	GKGCMC	1 cut(s)	2225
BstSNI	TACGTA	1 cut(s)	1838
BstUI	CGCG	1 cut(s)	952
BstV1I	GCAGC	2 cut(s)	3, 1459
BstV2I	GAAGAC	1 cut(s)	1408
BstX2I	RGATCY	2 cut(s)	745, 1459
BstYI	RGATCY	2 cut(s)	745, 1459
BstZ17I	GTATAC	1 cut(s)	1815
Bsu15I	ATCGAT	3 cut(s)	54, 1199, 1334
BsuI	GTATCC	1 cut(s)	2310
BsuRI	GGCC	3 cut(s)	1741, 2146, 2154
BsuTUI	ATCGAT	3 cut(s)	54, 1199, 1334
BtgI	CCRYGG	3 cut(s)	950, 1226, 2097
BtgZI	GCGATG	1 cut(s)	688
BtrI	CACGTC	3 cut(s)	265, 484, 730
BtsCI	GGATG	7 cut(s)	238, 1129, 1258, 1276, 1305, 1613, 2077
BtsI	GCAGTG	1 cut(s)	1240
BtsIMutI	CAGTG	5 cut(s)	331, 1045, 1119, 1240, 2225
Cac8I	GCNNGC	6 cut(s)	345, 1247, 1347, 1477, 1702, 1706
CciI	TCATGA	2 cut(s)	892, 1986
Cfr10I	RCCGGY	2 cut(s)	319, 2146
Cfr13I	GGNCC	5 cut(s)	714, 772, 1214, 1256, 1360
Cfr42I	CCGCGG	1 cut(s)	953
ClaI	ATCGAT	3 cut(s)	54, 1199, 1334
CseI	GACGC	2 cut(s)	590, 1538
CsiI	ACCWGGT	1 cut(s)	1753
Csp6I	GTAC	5 cut(s)	452, 1143, 1324, 1379, 2029
CspAI	ACCGGT	1 cut(s)	319
CviAII	CATG	9 cut(s)	23, 156, 226, 449, 470, 893, 1160, 1987, 2098
CviQI	GTAC	5 cut(s)	452, 1143, 1324, 1379, 2029
DdeI	CTNAG	4 cut(s)	406, 2287, 2333, 2460
DraI	TTTAAA	1 cut(s)	1632
EaeI	YGGCCR	1 cut(s)	2152
Eam1104I	CTCTTC	2 cut(s)	654, 2229
EarI	CTCTTC	2 cut(s)	654, 2229
Ecl136II	GAGCTC	1 cut(s)	1967
Eco105I	TACGTA	1 cut(s)	1838
Eco130I	CCWWGG	2 cut(s)	430, 2097
Eco24I	GRGCYC	1 cut(s)	1969
Eco31I	GGTCTC	2 cut(s)	415, 754
Eco32I	GATATC	2 cut(s)	403, 2175
Eco47I	GGWCC	5 cut(s)	714, 772, 1214, 1256, 1360
Eco53kI	GAGCTC	1 cut(s)	1967
Eco57I	CTGAAG	3 cut(s)	815, 1764, 2152
Eco72I	CACGTG	2 cut(s)	1220, 1466
Eco88I	CYCGRG	1 cut(s)	1889
EcoICRI	GAGCTC	1 cut(s)	1967
EcoO109I	RGGNCCY	1 cut(s)	772
EcoRI	GAATTC	1 cut(s)	2390
EcoRII	CCWGG	1 cut(s)	1753
EcoRV	GATATC	2 cut(s)	403, 2175
EcoT14I	CCWWGG	2 cut(s)	430, 2097
EcoT22I	ATGCAT	1 cut(s)	2215
EcoT38I	GRGCYC	1 cut(s)	1969
ErhI	CCWWGG	2 cut(s)	430, 2097
Esp3I	CGTCTC	3 cut(s)	226, 858, 867
FaeI	CATG	9 cut(s)	26, 159, 229, 452, 473, 896, 1163, 1990, 2101
FaqI	GGGAC	5 cut(s)	785, 854, 1346, 2292, 2398
FatI	CATG	9 cut(s)	22, 155, 225, 448, 469, 892, 1159, 1986, 2097
FauI	CCCGC	1 cut(s)	1371
FbaI	TGATCA	1 cut(s)	895
FblI	GTMKAC	2 cut(s)	267, 1814
Fnu4HI	GCNGC	4 cut(s)	17, 91, 1263, 1473
FokI	GGATG	7 cut(s)	225, 1116, 1265, 1283, 1312, 1620, 2084
FriOI	GRGCYC	1 cut(s)	1969
Fsp4HI	GCNGC	4 cut(s)	17, 91, 1263, 1473
FspBI	CTAG	8 cut(s)	200, 219, 330, 1592, 1617, 1781, 1863, 2115
GluI	GCNGC	4 cut(s)	17, 91, 1263, 1473
GsaI	CCCAGC	1 cut(s)	2385
HaeIII	GGCC	3 cut(s)	1741, 2146, 2154
HgaI	GACGC	2 cut(s)	590, 1538
Hin1I	GRCGYC	1 cut(s)	235
Hin1II	CATG	9 cut(s)	26, 159, 229, 452, 473, 896, 1163, 1990, 2101
HinfI	GANTC	7 cut(s)	78, 124, 152, 889, 955, 1729, 1973
HphI	GGTGA	4 cut(s)	364, 980, 1166, 1928
Hpy188I	TCNGA	3 cut(s)	1374, 1954, 2343
Hpy99I	CGWCG	2 cut(s)	731, 1983
HpyAV	CCTTC	9 cut(s)	268, 522, 526, 548, 1062, 1314, 1391, 1439, 1739
HpyCH4III	ACNGT	8 cut(s)	304, 823, 878, 1040, 1123, 1227, 1360, 1408
HpyF10VI	GCNNNNNNNGC	5 cut(s)	16, 1346, 2102, 2229, 2355
HpyF3I	CTNAG	4 cut(s)	406, 2287, 2333, 2460
Hsp92I	GRCGYC	1 cut(s)	235
Hsp92II	CATG	9 cut(s)	26, 159, 229, 452, 473, 896, 1163, 1990, 2101
Kpn2I	TCCGGA	2 cut(s)	958, 1930
KpnI	GGTACC	1 cut(s)	1327
Ksp22I	TGATCA	1 cut(s)	895
KspI	CCGCGG	1 cut(s)	953
LmnI	GCTCC	4 cut(s)	7, 106, 1604, 2102
Lsp1109I	GCAGC	2 cut(s)	3, 1459
LweI	GCATC	8 cut(s)	73, 652, 661, 819, 1138, 1205, 1772, 1843
MabI	ACCWGGT	1 cut(s)	1753
MaeI	CTAG	8 cut(s)	200, 219, 330, 1592, 1617, 1781, 1863, 2115
MaeIII	GTNAC	9 cut(s)	249, 358, 472, 565, 610, 730, 817, 2200, 2368
MbiI	CCGCTC	1 cut(s)	1708
MfeI	CAATTG	1 cut(s)	11
MflI	RGATCY	2 cut(s)	745, 1459
MhlI	GDGCHC	2 cut(s)	1969, 2225
MlsI	TGGCCA	1 cut(s)	2154
MluCI	AATT	9 cut(s)	11, 82, 487, 788, 1282, 1627, 1948, 2390, 2434
MluNI	TGGCCA	1 cut(s)	2154
MlyI	GAGTC	3 cut(s)	118, 146, 883
MmeI	TCCRAC	1 cut(s)	1382
Mox20I	TGGCCA	1 cut(s)	2154
Mph1103I	ATGCAT	1 cut(s)	2215
MroI	TCCGGA	2 cut(s)	958, 1930
MroXI	GAANNNNTTC	1 cut(s)	2394
MscI	TGGCCA	1 cut(s)	2154
MseI	TTAA	8 cut(s)	306, 594, 867, 1058, 1203, 1539, 1631, 2253
MslI	CAYNNNNRTG	6 cut(s)	453, 470, 728, 929, 1164, 2218
Msp20I	TGGCCA	1 cut(s)	2154
MspA1I	CMGCKG	1 cut(s)	952
MspR9I	CCNGG	4 cut(s)	765, 1755, 1898, 2322
MunI	CAATTG	1 cut(s)	11
Mva1269I	GAATGC	1 cut(s)	1521
MvaI	CCWGG	1 cut(s)	1755
MvnI	CGCG	1 cut(s)	952
MwoI	GCNNNNNNNGC	5 cut(s)	16, 1346, 2102, 2229, 2355
NciI	CCSGG	3 cut(s)	765, 1898, 2322
NcoI	CCATGG	1 cut(s)	2097
NlaIII	CATG	9 cut(s)	26, 159, 229, 452, 473, 896, 1163, 1990, 2101
NlaIV	GGNNCC	9 cut(s)	108, 439, 773, 1325, 1362, 1451, 1461, 1759, 1895
NmuCI	GTSAC	3 cut(s)	472, 730, 817
NsiI	ATGCAT	1 cut(s)	2215
OliI	CACNNNNGTG	3 cut(s)	470, 728, 929
PagI	TCATGA	2 cut(s)	892, 1986
PcsI	WCGNNNNNNNCGW	2 cut(s)	594, 1701
PctI	GAATGC	1 cut(s)	1521
PdmI	GAANNNNTTC	1 cut(s)	2394
PfeI	GAWTC	4 cut(s)	78, 955, 1729, 1973
PinAI	ACCGGT	1 cut(s)	319
PkrI	GCNGC	4 cut(s)	18, 92, 1264, 1474
PleI	GAGTC	3 cut(s)	118, 146, 883
PmaCI	CACGTG	2 cut(s)	1220, 1466
PmlI	CACGTG	2 cut(s)	1220, 1466
PpsI	GAGTC	3 cut(s)	118, 146, 883
Ppu21I	YACGTR	4 cut(s)	455, 1220, 1466, 1838
PpuMI	RGGWCCY	1 cut(s)	772
PsiI	TTATAA	1 cut(s)	1490
Psp124BI	GAGCTC	1 cut(s)	1969
Psp1406I	AACGTT	2 cut(s)	70, 1877
Psp5II	RGGWCCY	1 cut(s)	772
Psp6I	CCWGG	1 cut(s)	1753
PspCI	CACGTG	2 cut(s)	1220, 1466
PspFI	CCCAGC	1 cut(s)	2381
PspGI	CCWGG	1 cut(s)	1753
PspN4I	GGNNCC	9 cut(s)	108, 439, 773, 1325, 1362, 1451, 1461, 1759, 1895
PspPI	GGNCC	5 cut(s)	714, 772, 1214, 1256, 1360
PspPPI	RGGWCCY	1 cut(s)	772
PsrI	GAACNNNNNNTAC	2 cut(s)	1741, 1773
PsuI	RGATCY	2 cut(s)	745, 1459
RsaI	GTAC	5 cut(s)	453, 1144, 1325, 1380, 2030
RsaNI	GTAC	5 cut(s)	452, 1143, 1324, 1379, 2029
RseI	CAYNNNNRTG	6 cut(s)	453, 470, 728, 929, 1164, 2218
SacI	GAGCTC	1 cut(s)	1969
SacII	CCGCGG	1 cut(s)	953
SaqAI	TTAA	8 cut(s)	306, 594, 867, 1058, 1203, 1539, 1631, 2253
SatI	GCNGC	4 cut(s)	17, 91, 1263, 1473
Sau96I	GGNCC	5 cut(s)	714, 772, 1214, 1256, 1360
SchI	GAGTC	3 cut(s)	118, 146, 883
ScrFI	CCNGG	4 cut(s)	765, 1755, 1898, 2322
SduI	GDGCHC	2 cut(s)	1969, 2225
SexAI	ACCWGGT	1 cut(s)	1753
SfaNI	GCATC	8 cut(s)	73, 652, 661, 819, 1138, 1205, 1772, 1843
SfcI	CTRYAG	2 cut(s)	384, 1122
Sfr303I	CCGCGG	1 cut(s)	953
SgrBI	CCGCGG	1 cut(s)	953
SinI	GGWCC	5 cut(s)	714, 772, 1214, 1256, 1360
SmiI	ATTTAAAT	1 cut(s)	1632
SmiMI	CAYNNNNRTG	6 cut(s)	453, 470, 728, 929, 1164, 2218
SmlI	CTYRAG	2 cut(s)	776, 1968
SmoI	CTYRAG	2 cut(s)	776, 1968
SnaBI	TACGTA	1 cut(s)	1838
Sse9I	AATT	9 cut(s)	11, 82, 487, 788, 1282, 1627, 1948, 2390, 2434
SspI	AATATT	3 cut(s)	507, 1586, 2160
SspMI	CTAG	8 cut(s)	200, 219, 330, 1592, 1617, 1781, 1863, 2115
SstI	GAGCTC	1 cut(s)	1969
StyD4I	CCNGG	4 cut(s)	763, 1753, 1896, 2320
StyI	CCWWGG	2 cut(s)	430, 2097
SwaI	ATTTAAAT	1 cut(s)	1632
TaaI	ACNGT	8 cut(s)	304, 823, 878, 1040, 1123, 1227, 1360, 1408
TaqII	GACCGA	1 cut(s)	405
TasI	AATT	9 cut(s)	11, 82, 487, 788, 1282, 1627, 1948, 2390, 2434
TatI	WGTACW	2 cut(s)	1378, 2028
TauI	GCSGC	2 cut(s)	93, 1265
TfiI	GAWTC	4 cut(s)	78, 955, 1729, 1973
Tru1I	TTAA	8 cut(s)	306, 594, 867, 1058, 1203, 1539, 1631, 2253
Tru9I	TTAA	8 cut(s)	306, 594, 867, 1058, 1203, 1539, 1631, 2253
TscAI	CASTG	5 cut(s)	331, 1045, 1126, 1247, 2225
TseFI	GTSAC	3 cut(s)	472, 730, 817
TseI	GCWGC	2 cut(s)	16, 1472
Tsp45I	GTSAC	3 cut(s)	472, 730, 817
TspDTI	ATGAA	7 cut(s)	11, 330, 1148, 1316, 1407, 1555, 1975
TspGWI	ACGGA	5 cut(s)	20, 164, 706, 769, 1722
TspRI	CASTG	5 cut(s)	331, 1045, 1126, 1247, 2225
VpaK11BI	GGWCC	5 cut(s)	714, 772, 1214, 1256, 1360
XapI	RAATTY	2 cut(s)	1627, 2390
XbaI	TCTAGA	1 cut(s)	1616
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1224
XmiI	GTMKAC	2 cut(s)	267, 1814
XmnI	GAANNNNTTC	1 cut(s)	2394
XspI	CTAG	8 cut(s)	200, 219, 330, 1592, 1617, 1781, 1863, 2115
ZraI	GACGTC	1 cut(s)	236
Zsp2I	ATGCAT	1 cut(s)	2215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.