FvH4_1g03170

Phospholipase D alpha 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010738
Physical Location & Seq
Reverse (-)
1781634 .. 1785562
3929 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03170.t1

Sequence Viewer

Length: 2430 bp
ATGGCGCAAACTGTGCTGCTGCATGGAGTTCTTCATGCAACGATTTTCGAAGTCGATAGTTTGAAGGCCGGAGGTTGCTGCACCTTCTTTTGCAAGCTGTTTGAAGAGTCAGTAGGGCTTGGGAAAGGTTGCAGACCCTATGCAACCATTGATTTGGAAAATGCAAGAGTCGGGAGAACTAGACTGCTGGAAGAAACAACCACAAATCCACAGTGGAATGAAGCCTTTCACATTTACTGTGCTCATATGGCTTCTAATGTTGTTTTCTCCATCAAAGAATATAATCCAATTGGGGCTAATGTCATTGGAGAAGCTTATTTGCCAGCAGCTGAACTCTTCAATGGAGAAGAAGTAGACAGATGGCTTAAGATCACAATGCATCATCACGGTAGACCTTTACATGGAGGTTCTAAAATTCGTGTGAAACTGAGGTTTTATCCTGTAACAGGAAACTGTAATTGGTCTCATGGAATTAAAAGTCCTAAATATCCCGGAGTACCCTACACATTTTTCCCACAGAGAAATGGCTGCAGGGTTACCCTTTACCAAGATGCTCATGTTCCTGACAATTTCATCCCTGATATTCCTTTAGCTGGAGGCAAGTGTTACGAACCTCATCGGTGTTGGGAAGATATTTTTCATGCAATTTATAATGCTAAGCACTTGATATATATTGCTGGTTGGTCTGTGTATGCTAAAATCACATTAGTAAGGGACACAAAGAGGCCGAAGATAGTAGATGGAGACTTGACTCTGGGAGAGCTTCTTAAGAAAAAAGCAAAGGAAGGAGTGAAGGTCTTAATGCTTGTTTGGGATGACAGAACTTCTGTGAAGGTACTAAAGAGAGATGGCTTGATGGGTACTCATGATGAAGATACCGAAAGTTACTTCCAAGATACCGGAGTTCACTGCGTGTTGTGTCCCAGAAACCCTGACAATGGGCAAAGCATTGTTCAAGATATAGAGGTTGGAACAATGTTCACTCATCACCAGAAGATTGTTGTGGTGGACAGTGAATTGCCAAATGGAGCACCAGGGAAGAGGAGGATTGTGAGCTTCATTGGCGGCATTGATCTTTGTGATGGGAGATATGACACTCAATTTCATCCCCTTTTCAGGACTTTAGGCACAGTCAACCATGATGATCTCCATCAACCCAACTTTAATGGTGCCTCAATTGCGAAAGGCGGACCAAGAGAGCCTTGGCATGACATACATTGTAGGTTAGAAGGGCCAATTGCTTGGGATGTTTTGTTCAACTTTGAGCAAAGATGGAGAATGCAAGGTGTGAAGGACTTGCTTTTTCAACTCAGAGATTTTGATGAGACCTTCATACCTCCATCTCCGGTTATGTATCCTGAAGATCATGACACATGGAATGTTCAATTGTTCCGATCCATTGATGGAGGAGCTGCATTTGGATTTCCCAACTCTCCTAAAGATGCTGCCATGGCTGGTTTAGTCAGTGGCAGAGATCATGTGATTGACAGAAGCATTCAGGATGCATATATCAATGCAATTCGTCGAGCAAAGAGTTTTATCTACATTGAAAATCAGTACTTTCTCGGAAGCTCTTTCAGCTGGCAGTCGGATAATGAAGATCTCAAAGTTGAGGACGTTGGCGCTTTGCATCTTATTCCAAAAGAACTGTCACTAAAGATTGTTAGCAAAATTGAAGCTGGAGAAAGATTTACTGTCTACATTGTCATGCCTATGTGGCCAGAGGGTGTTCCAGAAAGCCAATCTGTTCAGGCAATACTGCATTGGCAGAAGTTGACGATGGAGATGATGTACAAGGACATAACACAAGCTCTCAAGAACAAGAGCCTTGATGCAAATCCGAAGGAGTATTTGACATTCTTCTGCCTTGGAAATCGTGAGAAGAAGCGAAGCGGAGAATATGAACCTCTTGAGAAACCAGGAAATGGAACGAATTACAGTAGAGCTCAGCAATCTCGGCGGTTCATGATCTACGTGCATGCCAAGTGTATGATAGTTGATGATGAATACATAATCATTGGATCCGCCAACATAAATCAGAGATCAATGGACGGCGCCAGGGATACCGAAATTGCCATGGGAGCTTACCAACCATATCACCTATCGACCAATGTACCTGCCAGGGGCCAAATCCATGGCCTCAGAATGGCATTGTGGTATGAACACTTGGGGTTGCTCGACAACACCTTTCTTGAGCCTGAGAGTGTGGAATGTGTGACAAAGGTGAACCAGATTGCCGAAAAGCTATGGAAACTATATACAAGTGAAACCTTAGAGGGTGACTTGCCAGGACATTTGTTACCCTATCCCGTAAAAATTACTGAGAATGGAGACGTCACTGAGCTACCTGGGGTTGAATTTTTTCCAGACACCAAGGCTAAAGTTCTTGGAACCAGATCTGACTTGATTCCTTCAATCATCACTACTTAA

Protein Analysis

810

Amino Acids

91.61

Weight (kDa)

6.14

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 42 - 123 3.5e-10 C2 domain
PLDc PF00614 326 - 363 1.2e-06 Phospholipase D Active site motif
PLDc_2 PF13091 652 - 691 9.4e-07 PLD-like domain
PLDc PF00614 657 - 682 6.9e-07 Phospholipase D Active site motif
PLD_C PF12357 727 - 798 1.1e-29 Phospholipase D C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010738)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25370 AT5G25370 AT5G25370 AT5G25370
fragaria_vesca FvH4_1g03170
malus_domestica MD02G1030700.v1.1 MD15G1172300.v1.1
prunus_persica Prupe.7G243800_v2.0.a1
pyrus_communis pycom02g02560 pycom15g15370
rosa_chinensis RchiOBHm_Chr2g0088301
rosa_laevigata RLG00000015940
rosa_multiflora Rmu_sc0004235.1_g000036
rosa_roxburghii Rroxscaffold_2G00152760
rosa_rugosa Rorug01G0482700
rosa_samantha Rh2AG037800 Rh2BG037000 Rh2CG038100 Rh2DG037500
rosa_wichuraiana Rw2G002980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
AatII GACGTC 1 cut(s) 2337
Acc36I ACCTGC 1 cut(s) 2125
AccB1I GGYRCC 2 cut(s) 1169, 2054
AccB7I CCANNNNNTGG 1 cut(s) 1925
AccI GTMKAC 3 cut(s) 354, 391, 1698
AciI CCGC 5 cut(s) 1065, 1188, 1893, 1960, 2025
AclWI GGATC 3 cut(s) 1389, 2016, 2029
AcoI YGGCCR 1 cut(s) 1718
AcsI RAATTY 2 cut(s) 414, 2357
AcuI CTGAAG 1 cut(s) 1380
AcyI GRCGYC 2 cut(s) 2055, 2334
AdeI CACNNNGTG 1 cut(s) 913
AfaI GTAC 6 cut(s) 498, 837, 862, 1559, 1793, 2115
AfiI CCNNNNNNNGG 8 cut(s) 401, 446, 593, 938, 1116, 1925, 2123, 2146
AflII CTTAAG 2 cut(s) 365, 767
AjnI CCWGG 6 cut(s) 1033, 1918, 2057, 2120, 2287, 2347
AjuI GAANNNNNNNTTGG 2 cut(s) 442, 474
AloI GAACNNNNNNTCC 2 cut(s) 1238, 1270
Alw21I GWGCWC 3 cut(s) 244, 1033, 1948
Alw26I GTCTC 4 cut(s) 468, 738, 1319, 2325
AlwI GGATC 3 cut(s) 1389, 2016, 2029
AlwNI CAGNNNCTG 1 cut(s) 329
AoxI GGCC 6 cut(s) 66, 725, 1232, 1718, 2125, 2137
ApeKI GCWGC 7 cut(s) 16, 19, 78, 326, 528, 1412, 1445
ApoI RAATTY 2 cut(s) 414, 2357
ArsI GACNNNNNNTTYG 2 cut(s) 1634, 1666
Asp700I GAANNNNTTC 2 cut(s) 225, 2361
AspLEI GCGC 3 cut(s) 7, 1625, 2057
AspS9I GGNCC 3 cut(s) 1190, 1232, 2125
AsuC2I CCSGG 1 cut(s) 492
AsuHPI GGTGA 4 cut(s) 980, 2090, 2236, 2291
AsuII TTCGAA 1 cut(s) 48
AvaII GGWCC 1 cut(s) 1190
BaeI ACNNNNGTAYC 4 cut(s) 888, 921, 2097, 2130
BalI TGGCCA 1 cut(s) 1720
BamHI GGATCC 1 cut(s) 2021
BanI GGYRCC 2 cut(s) 1169, 2054
BanII GRGCYC 1 cut(s) 1948
Bbv12I GWGCWC 3 cut(s) 244, 1033, 1948
BbvI GCAGC 7 cut(s) 3, 6, 65, 338, 515, 1399, 1432
BceAI ACGGC 1 cut(s) 2068
BciT130I CCWGG 6 cut(s) 1035, 1920, 2059, 2122, 2289, 2349
BciVI GTATCC 2 cut(s) 1365, 2056
BcnI CCSGG 1 cut(s) 492
BcoDI GTCTC 4 cut(s) 468, 738, 1319, 2325
BfaI CTAG 1 cut(s) 180
BfmI CTRYAG 1 cut(s) 529
BfoI RGCGCY 2 cut(s) 1626, 2058
BfrI CTTAAG 2 cut(s) 365, 767
BfuAI ACCTGC 1 cut(s) 2125
BfuI GTATCC 2 cut(s) 1365, 2056
BglI GCCNNNNNGGC 1 cut(s) 1717
BglII AGATCT 2 cut(s) 1600, 2396
BisI GCNGC 8 cut(s) 17, 20, 79, 327, 529, 1066, 1413, 1446
BlpI GCTNAGC 2 cut(s) 657, 1947
BlsI GCNGC 8 cut(s) 18, 21, 80, 328, 530, 1067, 1414, 1447
BmcAI AGTACT 1 cut(s) 1559
Bme1390I CCNGG 7 cut(s) 492, 1035, 1920, 2059, 2122, 2289, 2349
Bme18I GGWCC 1 cut(s) 1190
BmgT120I GGNCC 3 cut(s) 1190, 1232, 2125
BmiI GGNNCC 5 cut(s) 1171, 2023, 2056, 2126, 2392
BmrFI CCNGG 7 cut(s) 492, 1035, 1920, 2059, 2122, 2289, 2349
BmsI GCATC 6 cut(s) 388, 541, 1432, 1492, 1639, 1822
BpmI CTGGAG 2 cut(s) 615, 1701
Bpu1102I GCTNAGC 2 cut(s) 657, 1947
Bpu14I TTCGAA 1 cut(s) 48
BpuEI CTTGAG 3 cut(s) 1799, 1931, 2213
BpuMI CCSGG 1 cut(s) 492
BsaAI YACGTR 1 cut(s) 1975
BsaBI GATNNNNATC 2 cut(s) 1149, 1836
BsaHI GRCGYC 2 cut(s) 2055, 2334
BsaI GGTCTC 2 cut(s) 468, 1319
BsaWI WCCGGW 2 cut(s) 899, 1345
BsaXI ACNNNNNCTCC 6 cut(s) 336, 366, 780, 810, 1775, 1805
Bsc4I CCNNNNNNNGG 8 cut(s) 401, 446, 593, 938, 1116, 1925, 2123, 2146
Bse8I GATNNNNATC 2 cut(s) 1149, 1836
BseBI CCWGG 6 cut(s) 1035, 1920, 2059, 2122, 2289, 2349
BseGI GGATG 5 cut(s) 573, 820, 1105, 1252, 1507
BseJI GATNNNNATC 2 cut(s) 1149, 1836
BseLI CCNNNNNNNGG 8 cut(s) 401, 446, 593, 938, 1116, 1925, 2123, 2146
BseMII CTCAG 7 cut(s) 419, 1324, 1961, 2155, 2190, 2313, 2331
BseRI GAGGAG 2 cut(s) 1057, 1422
BseXI GCAGC 7 cut(s) 3, 6, 65, 338, 515, 1399, 1432
BsgI GTGCAG 1 cut(s) 64
BshFI GGCC 6 cut(s) 68, 727, 1234, 1720, 2127, 2139
BshNI GGYRCC 2 cut(s) 1169, 2054
BsiHKAI GWGCWC 3 cut(s) 244, 1033, 1948
BsiSI CCGG 4 cut(s) 69, 492, 900, 1346
BslFI GGGAC 2 cut(s) 728, 906
BslI CCNNNNNNNGG 8 cut(s) 401, 446, 593, 938, 1116, 1925, 2123, 2146
BsmAI GTCTC 4 cut(s) 468, 738, 1319, 2325
BsmBI CGTCTC 1 cut(s) 2325
BsmFI GGGAC 2 cut(s) 728, 906
BsmI GAATGC 2 cut(s) 1284, 1494
BsnI GGCC 6 cut(s) 68, 727, 1234, 1720, 2127, 2139
Bso31I GGTCTC 2 cut(s) 468, 1319
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 3 cut(s) 244, 1033, 1948
Bsp1407I TGTACA 1 cut(s) 1791
Bsp1720I GCTNAGC 2 cut(s) 657, 1947
Bsp19I CCATGG 3 cut(s) 1449, 2076, 2134
BspACI CCGC 5 cut(s) 1065, 1188, 1893, 1960, 2025
BspANI GGCC 6 cut(s) 68, 727, 1234, 1720, 2127, 2139
BspCNI CTCAG 7 cut(s) 420, 1323, 1960, 2154, 2191, 2314, 2332
BspHI TCATGA 3 cut(s) 865, 1366, 1965
BspLI GGNNCC 5 cut(s) 1171, 2023, 2056, 2126, 2392
BspMAI CTGCAG 1 cut(s) 533
BspMI ACCTGC 1 cut(s) 2125
BspPI GGATC 3 cut(s) 1389, 2016, 2029
BspT104I TTCGAA 1 cut(s) 48
BspT107I GGYRCC 2 cut(s) 1169, 2054
BspTI CTTAAG 2 cut(s) 365, 767
BspTNI GGTCTC 2 cut(s) 468, 1319
BsrGI TGTACA 1 cut(s) 1791
BssNI GRCGYC 2 cut(s) 2055, 2334
BssT1I CCWWGG 6 cut(s) 1202, 1449, 1867, 2076, 2134, 2373
Bst2UI CCWGG 6 cut(s) 1035, 1920, 2059, 2122, 2289, 2349
Bst6I CTCTTC 3 cut(s) 99, 341, 1034
BstACI GRCGYC 2 cut(s) 2055, 2334
BstAFI CTTAAG 2 cut(s) 365, 767
BstAPI GCANNNNNTGC 1 cut(s) 13
BstAUI TGTACA 1 cut(s) 1791
BstBAI YACGTR 1 cut(s) 1975
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 4 cut(s) 95, 324, 1583, 1980
BstDEI CTNAG 9 cut(s) 428, 657, 1310, 1947, 2141, 2199, 2272, 2322, 2340
BstDSI CCRYGG 3 cut(s) 1449, 2076, 2134
BstEII GGTNACC 1 cut(s) 535
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 5 cut(s) 573, 820, 1105, 1252, 1507
BstH2I RGCGCY 2 cut(s) 1626, 2058
BstHHI GCGC 3 cut(s) 7, 1625, 2057
BstMAI GTCTC 4 cut(s) 468, 738, 1319, 2325
BstMWI GCNNNNNNNGC 9 cut(s) 13, 248, 1062, 1178, 1451, 1578, 1717, 1957, 2081
BstNI CCWGG 6 cut(s) 1035, 1920, 2059, 2122, 2289, 2349
BstNSI RCATGY 1 cut(s) 1982
BstPI GGTNACC 1 cut(s) 535
BstSCI CCNGG 7 cut(s) 490, 1033, 1918, 2057, 2120, 2287, 2347
BstSFI CTRYAG 1 cut(s) 529
BstV1I GCAGC 7 cut(s) 3, 6, 65, 338, 515, 1399, 1432
BstX2I RGATCY 3 cut(s) 1600, 2021, 2396
BstXI CCANNNNNNTGG 3 cut(s) 154, 1242, 2135
BstYI RGATCY 3 cut(s) 1600, 2021, 2396
BsuI GTATCC 2 cut(s) 1365, 2056
BsuRI GGCC 6 cut(s) 68, 727, 1234, 1720, 2127, 2139
BtgI CCRYGG 3 cut(s) 1449, 2076, 2134
BtsCI GGATG 5 cut(s) 573, 820, 1105, 1252, 1507
BtsI GCAGTG 1 cut(s) 907
BtsIMutI CAGTG 5 cut(s) 218, 907, 1018, 1471, 2337
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 4 cut(s) 95, 324, 1583, 1980
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 3 cut(s) 865, 1366, 1965
CfoI GCGC 3 cut(s) 7, 1625, 2057
Cfr13I GGNCC 3 cut(s) 1190, 1232, 2125
Csp6I GTAC 6 cut(s) 497, 836, 861, 1558, 1792, 2114
CviQI GTAC 6 cut(s) 497, 836, 861, 1558, 1792, 2114
DdeI CTNAG 9 cut(s) 428, 657, 1310, 1947, 2141, 2199, 2272, 2322, 2340
DinI GGCGCC 1 cut(s) 2056
DraIII CACNNNGTG 1 cut(s) 913
EaeI YGGCCR 1 cut(s) 1718
Eam1104I CTCTTC 3 cut(s) 99, 341, 1034
EarI CTCTTC 3 cut(s) 99, 341, 1034
EciI GGCGGA 2 cut(s) 1203, 2014
Ecl136II GAGCTC 1 cut(s) 1946
Eco130I CCWWGG 6 cut(s) 1202, 1449, 1867, 2076, 2134, 2373
Eco24I GRGCYC 1 cut(s) 1948
Eco31I GGTCTC 2 cut(s) 468, 1319
Eco47I GGWCC 1 cut(s) 1190
Eco53kI GAGCTC 1 cut(s) 1946
Eco57I CTGAAG 1 cut(s) 1380
Eco91I GGTNACC 1 cut(s) 535
EcoICRI GAGCTC 1 cut(s) 1946
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoO65I GGTNACC 1 cut(s) 535
EcoRII CCWGG 6 cut(s) 1033, 1918, 2057, 2120, 2287, 2347
EcoT14I CCWWGG 6 cut(s) 1202, 1449, 1867, 2076, 2134, 2373
EcoT22I ATGCAT 2 cut(s) 381, 1507
EcoT38I GRGCYC 1 cut(s) 1948
EgeI GGCGCC 1 cut(s) 2056
EheI GGCGCC 1 cut(s) 2056
ErhI CCWWGG 6 cut(s) 1202, 1449, 1867, 2076, 2134, 2373
Esp3I CGTCTC 1 cut(s) 2325
FalI AAGNNNNNCTT 2 cut(s) 1186, 1218
FaqI GGGAC 2 cut(s) 728, 906
FauNDI CATATG 1 cut(s) 246
FblI GTMKAC 3 cut(s) 354, 391, 1698
Fnu4HI GCNGC 8 cut(s) 17, 20, 79, 327, 529, 1066, 1413, 1446
FokI GGATG 5 cut(s) 560, 827, 1092, 1259, 1514
FriOI GRGCYC 1 cut(s) 1948
Fsp4HI GCNGC 8 cut(s) 17, 20, 79, 327, 529, 1066, 1413, 1446
FspBI CTAG 1 cut(s) 180
GlaI GCGC 3 cut(s) 6, 1624, 2056
GluI GCNGC 8 cut(s) 17, 20, 79, 327, 529, 1066, 1413, 1446
GsuI CTGGAG 2 cut(s) 615, 1701
HaeII RGCGCY 2 cut(s) 1626, 2058
HaeIII GGCC 6 cut(s) 68, 727, 1234, 1720, 2127, 2139
HapII CCGG 4 cut(s) 69, 492, 900, 1346
HhaI GCGC 3 cut(s) 7, 1625, 2057
Hin1I GRCGYC 2 cut(s) 2055, 2334
Hin6I GCGC 3 cut(s) 5, 1623, 2055
HinP1I GCGC 3 cut(s) 5, 1623, 2055
HincII GTYRAC 2 cut(s) 1135, 1776
HindII GTYRAC 2 cut(s) 1135, 1776
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 4 cut(s) 107, 168, 751, 2407
HpaII CCGG 4 cut(s) 69, 492, 900, 1346
HphI GGTGA 4 cut(s) 980, 2090, 2236, 2291
Hpy166II GTNNAC 9 cut(s) 355, 392, 907, 981, 1009, 1135, 1699, 1776, 2227
Hpy188I TCNGA 8 cut(s) 1313, 1394, 1568, 1591, 1842, 2040, 2144, 2401
Hpy8I GTNNAC 9 cut(s) 355, 392, 907, 981, 1009, 1135, 1699, 1776, 2227
Hpy99I CGWCG 1 cut(s) 1527
HpyCH4IV ACGT 3 cut(s) 1617, 1974, 2334
HpyF10VI GCNNNNNNNGC 9 cut(s) 13, 248, 1062, 1178, 1451, 1578, 1717, 1957, 2081
HpyF3I CTNAG 9 cut(s) 428, 657, 1310, 1947, 2141, 2199, 2272, 2322, 2340
HpySE526I ACGT 3 cut(s) 1617, 1974, 2334
Hsp92I GRCGYC 2 cut(s) 2055, 2334
HspAI GCGC 3 cut(s) 5, 1623, 2055
KasI GGCGCC 1 cut(s) 2054
LmnI GCTCC 3 cut(s) 1028, 1409, 2081
Lsp1109I GCAGC 7 cut(s) 3, 6, 65, 338, 515, 1399, 1432
LweI GCATC 6 cut(s) 388, 541, 1432, 1492, 1639, 1822
MaeI CTAG 1 cut(s) 180
MaeII ACGT 3 cut(s) 1617, 1974, 2334
MaeIII GTNAC 9 cut(s) 442, 535, 605, 884, 1650, 2215, 2279, 2298, 2335
MfeI CAATTG 4 cut(s) 288, 1176, 1236, 1385
MflI RGATCY 3 cut(s) 1600, 2021, 2396
MhlI GDGCHC 3 cut(s) 244, 1033, 1948
MlsI TGGCCA 1 cut(s) 1720
MluNI TGGCCA 1 cut(s) 1720
Mly113I GGCGCC 1 cut(s) 2055
MlyI GAGTC 3 cut(s) 116, 177, 745
MmeI TCCRAC 2 cut(s) 949, 1569
Mox20I TGGCCA 1 cut(s) 1720
Mph1103I ATGCAT 2 cut(s) 381, 1507
MroXI GAANNNNTTC 2 cut(s) 225, 2361
MscI TGGCCA 1 cut(s) 1720
MseI TTAA 6 cut(s) 366, 474, 768, 800, 1164, 2428
MslI CAYNNNNRTG 2 cut(s) 1706, 1712
Msp20I TGGCCA 1 cut(s) 1720
MspA1I CMGCKG 2 cut(s) 329, 1581
MspCI CTTAAG 2 cut(s) 365, 767
MspI CCGG 4 cut(s) 69, 492, 900, 1346
MspR9I CCNGG 7 cut(s) 492, 1035, 1920, 2059, 2122, 2289, 2349
MunI CAATTG 4 cut(s) 288, 1176, 1236, 1385
Mva1269I GAATGC 2 cut(s) 1284, 1494
MvaI CCWGG 6 cut(s) 1035, 1920, 2059, 2122, 2289, 2349
MwoI GCNNNNNNNGC 9 cut(s) 13, 248, 1062, 1178, 1451, 1578, 1717, 1957, 2081
NarI GGCGCC 1 cut(s) 2055
NciI CCSGG 1 cut(s) 492
NcoI CCATGG 3 cut(s) 1449, 2076, 2134
NdeI CATATG 1 cut(s) 246
NlaIV GGNNCC 5 cut(s) 1171, 2023, 2056, 2126, 2392
NmeAIII GCCGAG 1 cut(s) 1936
NmuCI GTSAC 4 cut(s) 1650, 2215, 2279, 2335
NsiI ATGCAT 2 cut(s) 381, 1507
NspI RCATGY 1 cut(s) 1982
NspV TTCGAA 1 cut(s) 48
PaeI GCATGC 1 cut(s) 1982
PagI TCATGA 3 cut(s) 865, 1366, 1965
PctI GAATGC 2 cut(s) 1284, 1494
PdmI GAANNNNTTC 2 cut(s) 225, 2361
PfeI GAWTC 1 cut(s) 2407
PflMI CCANNNNNTGG 1 cut(s) 1925
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 8 cut(s) 18, 21, 80, 328, 530, 1067, 1414, 1447
PleI GAGTC 3 cut(s) 115, 176, 745
PluTI GGCGCC 1 cut(s) 2058
PpsI GAGTC 3 cut(s) 115, 176, 745
Ppu21I YACGTR 1 cut(s) 1975
PsiI TTATAA 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 1948
Psp6I CCWGG 6 cut(s) 1033, 1918, 2057, 2120, 2287, 2347
PspEI GGTNACC 1 cut(s) 535
PspGI CCWGG 6 cut(s) 1033, 1918, 2057, 2120, 2287, 2347
PspN4I GGNNCC 5 cut(s) 1171, 2023, 2056, 2126, 2392
PspPI GGNCC 3 cut(s) 1190, 1232, 2125
PstI CTGCAG 1 cut(s) 533
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 3 cut(s) 1600, 2021, 2396
PvuII CAGCTG 2 cut(s) 329, 1581
RsaI GTAC 6 cut(s) 498, 837, 862, 1559, 1793, 2115
RsaNI GTAC 6 cut(s) 497, 836, 861, 1558, 1792, 2114
RseI CAYNNNNRTG 2 cut(s) 1706, 1712
SacI GAGCTC 1 cut(s) 1948
SaqAI TTAA 6 cut(s) 366, 474, 768, 800, 1164, 2428
SatI GCNGC 8 cut(s) 17, 20, 79, 327, 529, 1066, 1413, 1446
Sau96I GGNCC 3 cut(s) 1190, 1232, 2125
ScaI AGTACT 1 cut(s) 1559
SchI GAGTC 3 cut(s) 116, 177, 745
ScrFI CCNGG 7 cut(s) 492, 1035, 1920, 2059, 2122, 2289, 2349
SduI GDGCHC 3 cut(s) 244, 1033, 1948
SfaNI GCATC 6 cut(s) 388, 541, 1432, 1492, 1639, 1822
SfcI CTRYAG 1 cut(s) 529
SfoI GGCGCC 1 cut(s) 2056
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 1 cut(s) 1190
SmiMI CAYNNNNRTG 2 cut(s) 1706, 1712
SmlI CTYRAG 5 cut(s) 365, 767, 1814, 1910, 2192
SmoI CTYRAG 5 cut(s) 365, 767, 1814, 1910, 2192
SphI GCATGC 1 cut(s) 1982
SsiI CCGC 5 cut(s) 1065, 1188, 1893, 1960, 2025
SspDI GGCGCC 1 cut(s) 2054
SspMI CTAG 1 cut(s) 180
SstI GAGCTC 1 cut(s) 1948
StyD4I CCNGG 7 cut(s) 490, 1033, 1918, 2057, 2120, 2287, 2347
StyI CCWWGG 6 cut(s) 1202, 1449, 1867, 2076, 2134, 2373
TaiI ACGT 3 cut(s) 1620, 1977, 2337
TaqI TCGA 5 cut(s) 48, 54, 1525, 2105, 2178
TatI WGTACW 2 cut(s) 1557, 1791
TauI GCSGC 1 cut(s) 1068
TfiI GAWTC 1 cut(s) 2407
Tru1I TTAA 6 cut(s) 366, 474, 768, 800, 1164, 2428
Tru9I TTAA 6 cut(s) 366, 474, 768, 800, 1164, 2428
TscAI CASTG 5 cut(s) 218, 914, 1018, 1471, 2344
TseFI GTSAC 4 cut(s) 1650, 2215, 2279, 2335
TseI GCWGC 7 cut(s) 16, 19, 78, 326, 528, 1412, 1445
Tsp45I GTSAC 4 cut(s) 1650, 2215, 2279, 2335
TspRI CASTG 5 cut(s) 218, 914, 1018, 1471, 2344
Van91I CCANNNNNTGG 1 cut(s) 1925
Vha464I CTTAAG 2 cut(s) 365, 767
VpaK11BI GGWCC 1 cut(s) 1190
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 2 cut(s) 414, 2357
XceI RCATGY 1 cut(s) 1982
XcmI CCANNNNNNNNNTGG 1 cut(s) 1200
XmiI GTMKAC 3 cut(s) 354, 391, 1698
XmnI GAANNNNTTC 2 cut(s) 225, 2361
XspI CTAG 1 cut(s) 180
ZraI GACGTC 1 cut(s) 2335
ZrmI AGTACT 1 cut(s) 1559
Zsp2I ATGCAT 2 cut(s) 381, 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.