Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0010738

Physical Location & Seq

Genomic Coordinates Forward (+)

89617248 .. 89625221

Gene Structure 7974 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00152760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3705 bp

ATGCTCAAAGGCAAGCTCAAGCTCCCGGACAAGGCCAGGCGCGTGTTCCCGGACCGCCTCGGCAGCCTCGTCGCCGGCTTCGATGAAAAGGCGGACGACGACGGAGGGTCCGATTTGGTGCTCGAGTCGGGCGAGGAGCTCGAGACGATCCAGCCCAACGGGTACGGGTCGAGTGGAGAGAGCGAGATCGCGGATTTTGAGGAGGAGAAAGTTGGAAGGGAACCCGATTTGAGGGCCCGGCTGCCCAAACCCGACAGAGATGGGCCGGGTTCGGTTTCGGTTCTGGCGGCCATTGCCGCCGATGATAAAAGGTCCGATCTTGAGTATGAGGTTAATCAGCCAGAGGTAAATTTGGAAAAGTTACAGAGAATAGCGAGTTTAGGGCTTCCTGATGGAGGAGGTTTGCGAGCAACAGCCTGGAAGCTGCTTCTGGGTTATCTACCTTCTTCTCGTGAGTTATGGGAAAAAGAGCTAACGGAAAATCGACTAAAATATGCCAAGCTCAAAGAGGAGCTCCTTTTGAGTCCTTCAGAACTCGCAAGGAAAAATGGTGGTGCCTTGAGTTATTGCGACCCGCCTGCTGACAGTGCTGTTGATGGACCACTGAAGAGACGTGAAATTTCCCAGGAAGACCACCCTTTAAGTCTTGGTAAGGCTAGTGTTTGGCATCAGTACTTTCAGCATACAGAGATTGCCGAGCAGATAGACCGTGATTTACCACGCACACACCCAGATTTGAAATTCTTCTCAGGGGACTCATCATTCAGTAGAAAACACAGGGAAGCACTGAGAAATATTCTTCTCCTGTTTGCAAAACTAAATCCAGCAATCCGTTATGTGCAAGGCATGAATGAGGTCTTGGCACCAATATACTATGTATTCAGTACTGACCCTAATGAGCAAAACACTGCAAATGCAGAAGCAGATAGCTTTTCTTGCTTTGTTCGACTCTTGAGTGACTCAGTGGACCACTTTTGTGAACAACTGGATAACAGTGCAGTTGGTATACTCGCCACTCTTTCCCGCTTGTCAGACTTATTGAAAGCCAATGATGAGGAGCTTTGGAGGCATCTTGAGCTCACCACAAAGGTGAAACCACAATACTATGCATTCAGATGGATCACATTACTACTCACACAGGAATTCAATTTCCAATCCATCATGAGAATCTGGGATTCACTCTTGGGATATGCTTCTGAAAATTTGTTGCGCGATGCTGCTATGCATGAAAAGCAGACTCTTGAGTGTTCATTTGTGGCAACCAGCCTTGAACTCAAGGTCAACATTCAAACGTTTGAAGATCCTAGTCAATGTGATCAATGGTGGCTACCCTGTAAGGACAGCACACAATTCCTATTATTGAATTTTGAGCTGATTGAAGAGTCAGTTGGGTTGGGGAAAGGTTGCAGACCCTATGCAACCATTGATTTTGAAAATGCAAGAGTTGGGAGAACTAGACTGCTGGAAGAAACAACCACAAATCCACAGTGGAATGAATCCTTTCACGTTTACTGTGCTCATATGGCTTCTAATGTCGTTTTCTCCATCAAAGAATACAATCCAATCGGGGCCAATGTCATCGGAAGAGCTTATTTGCCTGCTGCTGAACTCCTCAATGGAGAAGAAGTAGACAGATGGCTTAAGATCAGAATGGACCATCACAGTAGACCTTTACATGGAGGTTCCAAAATCCATGTGAAACTGAGGTTTCATCCTGTAACTAAAAACTGTAATTGGTCTCATGGAATCAAAAGTCCTAAATATCCAGGAGTACCCTACACATTTTTCCCACAAAGAAATGGCTGCAGGGTTACCCTTTACCAAGATGCTCATGTTCCTGACAATTTCATCCCCAATATCCCTTTAGCTGGAGGCAAGTGTTACGAACGTCATCGGTGTTGGGAAGATATTTTTCACGCAATCTATAATGCAAAGCACTTGATATACATTGCTGGCTGGTCTGTATATACTAAAATCACATTAGTAAGGGACACGATGAGGCCAAAGATAGTAGACGGAGACTTGACTCTGGGAGAGCTTCTTAAGAAGAAAGCAAAGGAAGGAGTGAAGGTCTTAATGCTTGTATGGGATGACAGAACTTCAGTGAAGGTACTAAAGAAAGACGGACTGATGGCTACTCATGATGAAGATACCGAAAGTTACTTCCATGACAGCGGAGTTCACTGCGTGTTGTGCCCCAGAAACCCTGACAATGGGCAAAGCATTGTTCAAGATATAGAGGTTGGAACAATGTTCACTCATCATCAAAAGATTGTGGTAGTGGACAGTGAATTGCCTAATAGAGCATTAGAAAAGAGGAGGATTGTGAGCTTCATTGGCGGCATTGATCTTTGTGATGGGAGATATGACACTCAGTTTCATCCCCTTTTCAGGACTTTAGGCACAATCCACCATGATGATCTCCATCAACCCAATTTTAATGGTGCCTCAATCGCAAAAGGTGGACCAAGGGAGCCTTGGCATGACATACATTGTAGGCTAGAAGGGCCTATAGCTTGGGATGTTTTATTCAACTTTGAGCAGAGGTGGAGAAAGCAAGGTGTGAAAGACTTACTTTTTCAACTCAGAGATTTTGATGAGACCTTTATACCACCATCTCCGGTTATGTATCCTGAAGACCATGACACATGGAATGTTCAGTTGTTCCGATCCATTGATGGAGGAGCTGCATTCGGATTTCCCAACTCTCCTGAAGATGCTACCAGGGCTGGTCTAGTCAGTGGAAGAGATCATGTGATTGACAGAAGCATTCAGGATGCATATATCAATGCAATTCGTCGAGCAAAGAGTTTTATCTACATCGAAAATCAGTACTTTCTTGGAAGCTCTTTCGGCTGGCAGTCAGATCATAAAGACCTCAAAGTTGAGGAGGTTGGGGCTTTGCATCTTATTCCAAAGGAACTATCACTAAAGATTGTTAGTAAGATTGAAGCTGGAGAAAGATTTACTGTCTACATTGTCATGCCTATGTGGCCAGAGGGTGTTCCAGAAAGCCAATCTGTTCAGGCAATATTGCATTGGCAGAAGTTGACAATGGAGATGATGTATAGGGACATAACACAAGCTCTAAAGACCAAGGGGCTTGATGCAAACCCTAAGGAGTATTTGACATTCTTTTGCCTTGGAAATCGTGAGAAGAAGCGAAGCGGAGAATATGAACCTCATGAGAAACCACAGCATGATACAGATTACAGTAGAGCTCAACAATCTAGGCGGTTCATGATCTACGTACATGCCAAGTGTATGATAGTTGATGATGAATACATAATCATTGGATCCGCCAACATAAATCAGAGATCAATGGACGGCGCCAGGGATACCGAGATTGCCATGGGAGCTTACCAACCATATCACCTATCTACCAATGTACCTGCCCGGGGCCAAATCCATGGCCTCAGAATGGCATTGTGGTATGAACATTTGGGGTTGCTCAATAACACCTTTCTTGAGCCGGAGAGTGTGGAATGTGTGAGAAAGGTGAACCAGATCGCCGAAAAGCATTGGGAACTCTATTTAAGTGAGACCTTAGAGGGTGACTTGCCAGGACATTTGCTACCCTATCCCGTAAAAATTACTGAAAATGGAGAGGTCACTGAGCTACCTGGGGTTGAATTTTTTCCAGACACCAAGGCTAAAGTTCATGGAACCAGATCTGACTTAATTCCTTCAATCATCACTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

1234

Amino Acids

140.57

Weight (kDa)

5.76

Isoelectric Point (pI)

39.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RabGAP-TBC	PF00566	227 - 395	8.4e-33	Rab-GTPase-TBC domain
RabGap-TBC_2	PF23436	262 - 404	2.1e-06	RabGAP TBC domain
C2	PF00168	467 - 548	9.1e-10	C2 domain
PLDc	PF00614	751 - 788	1.9e-06	Phospholipase D Active site motif
PLDc_2	PF13091	1080 - 1116	1.6e-06	PLD-like domain
PLDc	PF00614	1082 - 1107	1.1e-06	Phospholipase D Active site motif
PLD_C	PF12357	1153 - 1223	2e-29	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25370 AT5G25370 AT5G25370 AT5G25370
fragaria_vesca	FvH4_1g03170
malus_domestica	MD02G1030700.v1.1 MD15G1172300.v1.1
prunus_persica	Prupe.7G243800_v2.0.a1
pyrus_communis	pycom02g02560 pycom15g15370
rosa_chinensis	RchiOBHm_Chr2g0088301
rosa_laevigata	RLG00000015940
rosa_multiflora	Rmu_sc0004235.1_g000036
rosa_roxburghii	Rroxscaffold_2G00152760
rosa_rugosa	Rorug01G0482700
rosa_samantha	Rh2AG037800 Rh2BG037000 Rh2CG038100 Rh2DG037500
rosa_wichuraiana	Rw2G002980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	3400
AccB1I	GGYRCC	4 cut(s)	554, 862, 2444, 3329
AccI	GTMKAC	5 cut(s)	1006, 1629, 1666, 2013, 2973
AccII	CGCG	3 cut(s)	42, 191, 1212
AclI	AACGTT	1 cut(s)	1292
AclWI	GGATC	6 cut(s)	142, 1127, 1295, 2664, 3291, 3304
AcoI	YGGCCR	2 cut(s)	288, 2993
AcsI	RAATTY	7 cut(s)	349, 618, 740, 1142, 1201, 1363, 3632
AcuI	CTGAAG	5 cut(s)	513, 626, 2085, 2655, 2733
AcyI	GRCGYC	1 cut(s)	3330
AdeI	CACNNNGTG	1 cut(s)	2188
AfaI	GTAC	8 cut(s)	164, 674, 886, 1773, 2112, 2834, 3252, 3390
AfiI	CCNNNNNNNGG	9 cut(s)	31, 231, 395, 1676, 1868, 2213, 2391, 3398, 3421
AflII	CTTAAG	2 cut(s)	1640, 2042
AhdI	GACNNNNNGTC	1 cut(s)	106
AjiI	CACGTC	1 cut(s)	614
AjnI	CCWGG	8 cut(s)	35, 416, 624, 1765, 2723, 3332, 3562, 3622
AjuI	GAANNNNNNNTTGG	4 cut(s)	842, 874, 1371, 1403
AleI	CACNNNNGTG	2 cut(s)	975, 1088
Alw21I	GWGCWC	6 cut(s)	123, 141, 516, 1080, 1519, 3223
Alw26I	GTCTC	6 cut(s)	137, 604, 1743, 2013, 2594, 3536
AlwI	GGATC	6 cut(s)	142, 1127, 1295, 2664, 3291, 3304
Ama87I	CYCGRG	3 cut(s)	122, 140, 3396
ApaI	GGGCCC	1 cut(s)	238
ApeKI	GCWGC	7 cut(s)	63, 241, 424, 1217, 1601, 1803, 2687
ApoI	RAATTY	7 cut(s)	349, 618, 740, 1142, 1201, 1363, 3632
Asp700I	GAANNNNTTC	3 cut(s)	743, 1500, 3636
AspLEI	GCGC	3 cut(s)	42, 1212, 3332
AsuC2I	CCSGG	6 cut(s)	26, 50, 238, 267, 3397, 3398
AsuHPI	GGTGA	5 cut(s)	1072, 1102, 3365, 3511, 3566
AvaI	CYCGRG	3 cut(s)	122, 140, 3396
AvaII	GGWCC	7 cut(s)	52, 108, 312, 599, 967, 1654, 2465
AxyI	CCTNAGG	1 cut(s)	3117
BaeGI	GKGCMC	2 cut(s)	238, 2198
BaeI	ACNNNNGTAYC	2 cut(s)	3372, 3405
BalI	TGGCCA	1 cut(s)	2995
BamHI	GGATCC	1 cut(s)	3296
BanI	GGYRCC	4 cut(s)	554, 862, 2444, 3329
BanII	GRGCYC	5 cut(s)	141, 238, 516, 1080, 3223
BauI	CACGAG	1 cut(s)	450
BbsI	GAAGAC	2 cut(s)	636, 2643
Bbv12I	GWGCWC	6 cut(s)	123, 141, 516, 1080, 1519, 3223
BbvI	GCAGC	7 cut(s)	75, 228, 411, 1204, 1588, 1790, 2674
BceAI	ACGGC	1 cut(s)	3343
BcgI	CGANNNNNNTGC	4 cut(s)	52, 86, 560, 594
BciT130I	CCWGG	8 cut(s)	37, 418, 626, 1767, 2725, 3334, 3564, 3624
BciVI	GTATCC	2 cut(s)	2640, 3331
BclI	TGATCA	1 cut(s)	1315
BcnI	CCSGG	6 cut(s)	26, 50, 238, 267, 3397, 3398
BcoDI	GTCTC	6 cut(s)	137, 604, 1743, 2013, 2594, 3536
BfaI	CTAG	6 cut(s)	657, 1305, 1455, 2501, 2735, 3231
BfmI	CTRYAG	2 cut(s)	1804, 2511
BfoI	RGCGCY	1 cut(s)	3333
BfrI	CTTAAG	2 cut(s)	1640, 2042
BfuAI	ACCTGC	1 cut(s)	3400
BfuI	GTATCC	2 cut(s)	2640, 3331
BglI	GCCNNNNNGGC	1 cut(s)	2992
BglII	AGATCT	1 cut(s)	3671
BmcAI	AGTACT	3 cut(s)	674, 886, 2834
Bme18I	GGWCC	7 cut(s)	52, 108, 312, 599, 967, 1654, 2465
BmeRI	GACNNNNNGTC	1 cut(s)	106
BmeT110I	CYCGRG	3 cut(s)	122, 140, 3396
BmgBI	CACGTC	1 cut(s)	614
BmsI	GCATC	8 cut(s)	676, 1078, 1204, 1816, 2707, 2767, 2914, 3097
BpiI	GAAGAC	2 cut(s)	636, 2643
BpmI	CTGGAG	2 cut(s)	1890, 2976
BpuEI	CTTGAG	7 cut(s)	341, 580, 973, 1094, 1259, 1262, 3488
BpuMI	CCSGG	6 cut(s)	26, 50, 238, 267, 3397, 3398
BsaAI	YACGTR	1 cut(s)	3250
BsaBI	GATNNNNATC	1 cut(s)	2424
BsaHI	GRCGYC	1 cut(s)	3330
BsaI	GGTCTC	3 cut(s)	1743, 2594, 3536
BsaWI	WCCGGW	1 cut(s)	2620
BsaXI	ACNNNNNCTCC	6 cut(s)	1611, 1641, 2055, 2085, 3050, 3080
Bsc4I	CCNNNNNNNGG	9 cut(s)	31, 231, 395, 1676, 1868, 2213, 2391, 3398, 3421
Bse118I	RCCGGY	1 cut(s)	74
Bse1I	ACTGG	1 cut(s)	990
Bse21I	CCTNAGG	1 cut(s)	3117
Bse3DI	GCAATG	2 cut(s)	291, 1947
Bse8I	GATNNNNATC	1 cut(s)	2424
BseBI	CCWGG	8 cut(s)	37, 418, 626, 1767, 2725, 3334, 3564, 3624
BseGI	GGATG	6 cut(s)	1711, 1848, 2095, 2380, 2527, 2782
BseJI	GATNNNNATC	1 cut(s)	2424
BseLI	CCNNNNNNNGG	9 cut(s)	31, 231, 395, 1676, 1868, 2213, 2391, 3398, 3421
BseMI	GCAATG	2 cut(s)	291, 1947
BseMII	CTCAG	8 cut(s)	762, 779, 975, 1694, 2387, 2599, 3430, 3606
BseNI	ACTGG	1 cut(s)	990
BseSI	GKGCMC	2 cut(s)	238, 2198
BseXI	GCAGC	7 cut(s)	75, 228, 411, 1204, 1588, 1790, 2674
BsgI	GTGCAG	1 cut(s)	1017
Bsh1236I	CGCG	3 cut(s)	42, 191, 1212
BshNI	GGYRCC	4 cut(s)	554, 862, 2444, 3329
BsiHKAI	GWGCWC	6 cut(s)	123, 141, 516, 1080, 1519, 3223
BsiHKCI	CYCGRG	3 cut(s)	122, 140, 3396
BsiSI	CCGG	8 cut(s)	26, 50, 75, 238, 266, 2621, 3397, 3473
BslFI	GGGAC	3 cut(s)	767, 2003, 3086
BslI	CCNNNNNNNGG	9 cut(s)	31, 231, 395, 1676, 1868, 2213, 2391, 3398, 3421
BsmAI	GTCTC	6 cut(s)	137, 604, 1743, 2013, 2594, 3536
BsmBI	CGTCTC	2 cut(s)	137, 604
BsmFI	GGGAC	3 cut(s)	767, 2003, 3086
BsmI	GAATGC	3 cut(s)	1109, 2690, 2769
Bso31I	GGTCTC	3 cut(s)	1743, 2594, 3536
BsoBI	CYCGRG	3 cut(s)	122, 140, 3396
Bsp120I	GGGCCC	1 cut(s)	234
Bsp1286I	GDGCHC	8 cut(s)	123, 141, 238, 516, 1080, 1519, 2198, 3223
Bsp19I	CCATGG	2 cut(s)	3351, 3409
BspCNI	CTCAG	8 cut(s)	761, 780, 974, 1695, 2386, 2598, 3429, 3607
BspFNI	CGCG	3 cut(s)	42, 191, 1212
BspHI	TCATGA	4 cut(s)	1161, 2140, 3184, 3240
BspMAI	CTGCAG	1 cut(s)	1808
BspMI	ACCTGC	1 cut(s)	3400
BspPI	GGATC	6 cut(s)	142, 1127, 1295, 2664, 3291, 3304
BspQI	GCTCTTC	1 cut(s)	1579
BspT107I	GGYRCC	4 cut(s)	554, 862, 2444, 3329
BspTI	CTTAAG	2 cut(s)	1640, 2042
BspTNI	GGTCTC	3 cut(s)	1743, 2594, 3536
BsrDI	GCAATG	2 cut(s)	291, 1947
BsrFI	RCCGGY	1 cut(s)	74
BsrI	ACTGG	1 cut(s)	990
BssAI	RCCGGY	1 cut(s)	74
BssNAI	GTATAC	1 cut(s)	1007
BssNI	GRCGYC	1 cut(s)	3330
BssSI	CACGAG	1 cut(s)	450
BssT1I	CCWWGG	7 cut(s)	2468, 2477, 3096, 3142, 3351, 3409, 3648
Bst1107I	GTATAC	1 cut(s)	1007
Bst2BI	CACGAG	1 cut(s)	450
Bst2UI	CCWGG	8 cut(s)	37, 418, 626, 1767, 2725, 3334, 3564, 3624
Bst6I	CTCTTC	4 cut(s)	602, 1374, 1579, 2740
BstACI	GRCGYC	1 cut(s)	3330
BstAFI	CTTAAG	2 cut(s)	1640, 2042
BstBAI	YACGTR	1 cut(s)	3250
BstC8I	GCNNGC	7 cut(s)	14, 76, 408, 579, 1599, 1954, 2858
BstDSI	CCRYGG	2 cut(s)	3351, 3409
BstEII	GGTNACC	1 cut(s)	1810
BstENI	CCTNNNNNAGG	1 cut(s)	393
BstF5I	GGATG	6 cut(s)	1711, 1848, 2095, 2380, 2527, 2782
BstFNI	CGCG	3 cut(s)	42, 191, 1212
BstH2I	RGCGCY	1 cut(s)	3333
BstHHI	GCGC	3 cut(s)	42, 1212, 3332
BstMAI	GTCTC	6 cut(s)	137, 604, 1743, 2013, 2594, 3536
BstNI	CCWGG	8 cut(s)	37, 418, 626, 1767, 2725, 3334, 3564, 3624
BstNSI	RCATGY	1 cut(s)	3257
BstPI	GGTNACC	1 cut(s)	1810
BstSFI	CTRYAG	2 cut(s)	1804, 2511
BstSLI	GKGCMC	2 cut(s)	238, 2198
BstSNI	TACGTA	1 cut(s)	3250
BstUI	CGCG	3 cut(s)	42, 191, 1212
BstV1I	GCAGC	7 cut(s)	75, 228, 411, 1204, 1588, 1790, 2674
BstV2I	GAAGAC	2 cut(s)	636, 2643
BstX2I	RGATCY	3 cut(s)	1300, 3296, 3671
BstXI	CCANNNNNNTGG	1 cut(s)	3410
BstYI	RGATCY	3 cut(s)	1300, 3296, 3671
BstZ17I	GTATAC	1 cut(s)	1007
Bsu36I	CCTNAGG	1 cut(s)	3117
BsuI	GTATCC	2 cut(s)	2640, 3331
BtgI	CCRYGG	2 cut(s)	3351, 3409
BtgZI	GCGATG	1 cut(s)	1227
BtrI	CACGTC	1 cut(s)	614
BtsCI	GGATG	6 cut(s)	1711, 1848, 2095, 2380, 2527, 2782
BtsI	GCAGTG	2 cut(s)	906, 2182
BveI	ACCTGC	1 cut(s)	3400
Cac8I	GCNNGC	7 cut(s)	14, 76, 408, 579, 1599, 1954, 2858
CciI	TCATGA	4 cut(s)	1161, 2140, 3184, 3240
CfoI	GCGC	3 cut(s)	42, 1212, 3332
Cfr10I	RCCGGY	1 cut(s)	74
Cfr9I	CCCGGG	1 cut(s)	3396
CpoI	CGGWCCG	1 cut(s)	52
Csp6I	GTAC	8 cut(s)	163, 673, 885, 1772, 2111, 2833, 3251, 3389
CspI	CGGWCCG	1 cut(s)	52
CviQI	GTAC	8 cut(s)	163, 673, 885, 1772, 2111, 2833, 3251, 3389
DinI	GGCGCC	1 cut(s)	3331
DraIII	CACNNNGTG	1 cut(s)	2188
DriI	GACNNNNNGTC	1 cut(s)	106
EaeI	YGGCCR	2 cut(s)	288, 2993
Eam1104I	CTCTTC	4 cut(s)	602, 1374, 1579, 2740
Eam1105I	GACNNNNNGTC	1 cut(s)	106
EarI	CTCTTC	4 cut(s)	602, 1374, 1579, 2740
EciI	GGCGGA	2 cut(s)	107, 3289
Ecl136II	GAGCTC	4 cut(s)	139, 514, 1078, 3221
Eco105I	TACGTA	1 cut(s)	3250
Eco130I	CCWWGG	7 cut(s)	2468, 2477, 3096, 3142, 3351, 3409, 3648
Eco24I	GRGCYC	5 cut(s)	141, 238, 516, 1080, 3223
Eco31I	GGTCTC	3 cut(s)	1743, 2594, 3536
Eco47I	GGWCC	7 cut(s)	52, 108, 312, 599, 967, 1654, 2465
Eco53kI	GAGCTC	4 cut(s)	139, 514, 1078, 3221
Eco57I	CTGAAG	5 cut(s)	513, 626, 2085, 2655, 2733
Eco81I	CCTNAGG	1 cut(s)	3117
Eco88I	CYCGRG	3 cut(s)	122, 140, 3396
Eco91I	GGTNACC	1 cut(s)	1810
EcoICRI	GAGCTC	4 cut(s)	139, 514, 1078, 3221
EcoNI	CCTNNNNNAGG	1 cut(s)	393
EcoO109I	RGGNCCY	2 cut(s)	234, 2507
EcoO65I	GGTNACC	1 cut(s)	1810
EcoRI	GAATTC	1 cut(s)	1142
EcoRII	CCWGG	8 cut(s)	35, 416, 624, 1765, 2723, 3332, 3562, 3622
EcoT14I	CCWWGG	7 cut(s)	2468, 2477, 3096, 3142, 3351, 3409, 3648
EcoT22I	ATGCAT	3 cut(s)	1111, 1227, 2782
EcoT38I	GRGCYC	5 cut(s)	141, 238, 516, 1080, 3223
EgeI	GGCGCC	1 cut(s)	3331
EheI	GGCGCC	1 cut(s)	3331
ErhI	CCWWGG	7 cut(s)	2468, 2477, 3096, 3142, 3351, 3409, 3648
Esp3I	CGTCTC	2 cut(s)	137, 604
FalI	AAGNNNNNCTT	4 cut(s)	2461, 2493, 2559, 2591
FaqI	GGGAC	3 cut(s)	767, 2003, 3086
FauI	CCCGC	2 cut(s)	582, 1031
FauNDI	CATATG	1 cut(s)	1521
FbaI	TGATCA	1 cut(s)	1315
FblI	GTMKAC	5 cut(s)	1006, 1629, 1666, 2013, 2973
FokI	GGATG	6 cut(s)	1698, 1835, 2102, 2367, 2534, 2789
FriOI	GRGCYC	5 cut(s)	141, 238, 516, 1080, 3223
FspBI	CTAG	6 cut(s)	657, 1305, 1455, 2501, 2735, 3231
GlaI	GCGC	3 cut(s)	41, 1211, 3331
GsuI	CTGGAG	2 cut(s)	1890, 2976
HaeII	RGCGCY	1 cut(s)	3333
HapII	CCGG	8 cut(s)	26, 50, 75, 238, 266, 2621, 3397, 3473
HhaI	GCGC	3 cut(s)	42, 1212, 3332
Hin1I	GRCGYC	1 cut(s)	3330
Hin6I	GCGC	3 cut(s)	40, 1210, 3330
HinP1I	GCGC	3 cut(s)	40, 1210, 3330
HincII	GTYRAC	2 cut(s)	1282, 3051
HindII	GTYRAC	2 cut(s)	1282, 3051
HpaII	CCGG	8 cut(s)	26, 50, 75, 238, 266, 2621, 3397, 3473
HphI	GGTGA	5 cut(s)	1072, 1102, 3365, 3511, 3566
Hpy99I	CGWCG	4 cut(s)	74, 101, 104, 2802
HpyAV	CCTTC	8 cut(s)	210, 453, 537, 2054, 2062, 2101, 2498, 3696
HpyCH4IV	ACGT	5 cut(s)	613, 1292, 1506, 1888, 3249
HpySE526I	ACGT	5 cut(s)	613, 1292, 1506, 1888, 3249
Hsp92I	GRCGYC	1 cut(s)	3330
HspAI	GCGC	3 cut(s)	40, 1210, 3330
KasI	GGCGCC	1 cut(s)	3329
KroI	GCCGGC	1 cut(s)	74
KroNI	GCCGGC	1 cut(s)	76
Ksp22I	TGATCA	1 cut(s)	1315
LguI	GCTCTTC	1 cut(s)	1579
LmnI	GCTCC	8 cut(s)	27, 136, 511, 519, 1057, 2473, 2684, 3356
Lsp1109I	GCAGC	7 cut(s)	75, 228, 411, 1204, 1588, 1790, 2674
LweI	GCATC	8 cut(s)	676, 1078, 1204, 1816, 2707, 2767, 2914, 3097
MaeI	CTAG	6 cut(s)	657, 1305, 1455, 2501, 2735, 3231
MaeII	ACGT	5 cut(s)	613, 1292, 1506, 1888, 3249
MaeIII	GTNAC	8 cut(s)	360, 956, 1717, 1810, 1880, 2159, 3554, 3610
MflI	RGATCY	3 cut(s)	1300, 3296, 3671
MhlI	GDGCHC	8 cut(s)	123, 141, 238, 516, 1080, 1519, 2198, 3223
MlsI	TGGCCA	1 cut(s)	2995
MluNI	TGGCCA	1 cut(s)	2995
Mly113I	GGCGCC	1 cut(s)	3330
MlyI	GAGTC	8 cut(s)	134, 532, 749, 942, 953, 1231, 1391, 2020
MmeI	TCCRAC	2 cut(s)	193, 2224
Mox20I	TGGCCA	1 cut(s)	2995
Mph1103I	ATGCAT	3 cut(s)	1111, 1227, 2782
MroNI	GCCGGC	1 cut(s)	74
MroXI	GAANNNNTTC	3 cut(s)	743, 1500, 3636
MscI	TGGCCA	1 cut(s)	2995
MseI	TTAA	9 cut(s)	333, 641, 1641, 2043, 2075, 2439, 3536, 3680, 3703
MslI	CAYNNNNRTG	6 cut(s)	975, 1088, 1114, 2415, 2981, 2987
Msp20I	TGGCCA	1 cut(s)	2995
MspA1I	CMGCKG	1 cut(s)	2175
MspCI	CTTAAG	2 cut(s)	1640, 2042
MspI	CCGG	8 cut(s)	26, 50, 75, 238, 266, 2621, 3397, 3473
Mva1269I	GAATGC	3 cut(s)	1109, 2690, 2769
MvaI	CCWGG	8 cut(s)	37, 418, 626, 1767, 2725, 3334, 3564, 3624
MvnI	CGCG	3 cut(s)	42, 191, 1212
NaeI	GCCGGC	1 cut(s)	76
NarI	GGCGCC	1 cut(s)	3330
NciI	CCSGG	6 cut(s)	26, 50, 238, 267, 3397, 3398
NcoI	CCATGG	2 cut(s)	3351, 3409
NdeI	CATATG	1 cut(s)	1521
NgoMIV	GCCGGC	1 cut(s)	74
NmeAIII	GCCGAG	2 cut(s)	39, 721
NmuCI	GTSAC	3 cut(s)	956, 3554, 3610
NsiI	ATGCAT	3 cut(s)	1111, 1227, 2782
NspI	RCATGY	1 cut(s)	3257
OliI	CACNNNNGTG	2 cut(s)	975, 1088
PaeR7I	CTCGAG	2 cut(s)	122, 140
PagI	TCATGA	4 cut(s)	1161, 2140, 3184, 3240
PciSI	GCTCTTC	1 cut(s)	1579
PcsI	WCGNNNNNNNCGW	4 cut(s)	66, 78, 108, 129
PctI	GAATGC	3 cut(s)	1109, 2690, 2769
PdiI	GCCGGC	1 cut(s)	76
PdmI	GAANNNNTTC	3 cut(s)	743, 1500, 3636
PfeI	GAWTC	4 cut(s)	1167, 1175, 1496, 1746
PfoI	TCCNGGA	3 cut(s)	24, 48, 1765
PleI	GAGTC	8 cut(s)	133, 531, 749, 942, 953, 1231, 1390, 2020
PluTI	GGCGCC	1 cut(s)	3333
PpsI	GAGTC	8 cut(s)	133, 531, 749, 942, 953, 1231, 1390, 2020
Ppu21I	YACGTR	1 cut(s)	3250
Psp124BI	GAGCTC	4 cut(s)	141, 516, 1080, 3223
Psp1406I	AACGTT	1 cut(s)	1292
Psp6I	CCWGG	8 cut(s)	35, 416, 624, 1765, 2723, 3332, 3562, 3622
PspEI	GGTNACC	1 cut(s)	1810
PspGI	CCWGG	8 cut(s)	35, 416, 624, 1765, 2723, 3332, 3562, 3622
PspOMI	GGGCCC	1 cut(s)	234
PspXI	VCTCGAGB	1 cut(s)	122
PstI	CTGCAG	1 cut(s)	1808
PsuI	RGATCY	3 cut(s)	1300, 3296, 3671
RsaI	GTAC	8 cut(s)	164, 674, 886, 1773, 2112, 2834, 3252, 3390
RsaNI	GTAC	8 cut(s)	163, 673, 885, 1772, 2111, 2833, 3251, 3389
RseI	CAYNNNNRTG	6 cut(s)	975, 1088, 1114, 2415, 2981, 2987
Rsr2I	CGGWCCG	1 cut(s)	52
RsrII	CGGWCCG	1 cut(s)	52
SacI	GAGCTC	4 cut(s)	141, 516, 1080, 3223
SapI	GCTCTTC	1 cut(s)	1579
SaqAI	TTAA	9 cut(s)	333, 641, 1641, 2043, 2075, 2439, 3536, 3680, 3703
ScaI	AGTACT	3 cut(s)	674, 886, 2834
SchI	GAGTC	8 cut(s)	134, 532, 749, 942, 953, 1231, 1391, 2020
SduI	GDGCHC	8 cut(s)	123, 141, 238, 516, 1080, 1519, 2198, 3223
SfaNI	GCATC	8 cut(s)	676, 1078, 1204, 1816, 2707, 2767, 2914, 3097
SfcI	CTRYAG	2 cut(s)	1804, 2511
SfoI	GGCGCC	1 cut(s)	3331
Sfr274I	CTCGAG	2 cut(s)	122, 140
SinI	GGWCC	7 cut(s)	52, 108, 312, 599, 967, 1654, 2465
SlaI	CTCGAG	2 cut(s)	122, 140
SmaI	CCCGGG	1 cut(s)	3398
SmiMI	CAYNNNNRTG	6 cut(s)	975, 1088, 1114, 2415, 2981, 2987
SnaBI	TACGTA	1 cut(s)	3250
SspDI	GGCGCC	1 cut(s)	3329
SspI	AATATT	2 cut(s)	796, 3033
SspMI	CTAG	6 cut(s)	657, 1305, 1455, 2501, 2735, 3231
SstI	GAGCTC	4 cut(s)	141, 516, 1080, 3223
StyI	CCWWGG	7 cut(s)	2468, 2477, 3096, 3142, 3351, 3409, 3648
TaiI	ACGT	5 cut(s)	616, 1295, 1509, 1891, 3252
TaqI	TCGA	8 cut(s)	81, 123, 141, 170, 484, 946, 2800, 2823
TatI	WGTACW	3 cut(s)	672, 884, 2832
TauI	GCSGC	3 cut(s)	290, 299, 2343
TfiI	GAWTC	4 cut(s)	1167, 1175, 1496, 1746
Tru1I	TTAA	9 cut(s)	333, 641, 1641, 2043, 2075, 2439, 3536, 3680, 3703
Tru9I	TTAA	9 cut(s)	333, 641, 1641, 2043, 2075, 2439, 3536, 3680, 3703
TseFI	GTSAC	3 cut(s)	956, 3554, 3610
TseI	GCWGC	7 cut(s)	63, 241, 424, 1217, 1601, 1803, 2687
Tsp45I	GTSAC	3 cut(s)	956, 3554, 3610
TspGWI	ACGGA	5 cut(s)	117, 491, 821, 2031, 2139
TspMI	CCCGGG	1 cut(s)	3396
Vha464I	CTTAAG	2 cut(s)	1640, 2042
VpaK11BI	GGWCC	7 cut(s)	52, 108, 312, 599, 967, 1654, 2465
XagI	CCTNNNNNAGG	1 cut(s)	393
XapI	RAATTY	7 cut(s)	349, 618, 740, 1142, 1201, 1363, 3632
XceI	RCATGY	1 cut(s)	3257
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2475
XhoI	CTCGAG	2 cut(s)	122, 140
XmaI	CCCGGG	1 cut(s)	3396
XmiI	GTMKAC	5 cut(s)	1006, 1629, 1666, 2013, 2973
XmnI	GAANNNNTTC	3 cut(s)	743, 1500, 3636
XspI	CTAG	6 cut(s)	657, 1305, 1455, 2501, 2735, 3231
ZrmI	AGTACT	3 cut(s)	674, 886, 2834
Zsp2I	ATGCAT	3 cut(s)	1111, 1227, 2782

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.