Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0010738

Physical Location & Seq

Genomic Coordinates Reverse (-)

2584047 .. 2587034

Gene Structure 2988 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG037000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGGCGCAGACTGTGCTGCTGCATGGAGTACTTCATGCAACAATATTCGAAGTCGATAAGTTGAAGGCTGGAAATTGCTGCACCTTCATATGCAAGCTGATTGAAGAGTCAGTTGGGTTGGGGAAAGGTTGCAGACCCTATGCGACCATTGATTTGGAAAATGTAAGAGTTGGGAGAACTAGACTGCTGGAAGAAACAACCACAAATCCACAGTGGAATGAATCCTTTCACATTTACTGTGCTCATATGGCTTCTAATGTCGTTTTCTCCATCAAAGAATACAATCCAATCGGGGCCAATGTCATCGGAAGAGCTTATTTGCCTGCTGCTGAACTCCTCAATGGAGAAGAAGTAGACAGATGGCTTAAGATCAGAATGGATCATCACAGTAGACCTTTACATGGAGGTTCCAAAATCCATGTGAAACTGAGGTTTCATCCTGTAACTAAAAACTGTAATTGGTCTCATGGAATCAAAAGTCCTAAATTTCCAGGAGTACCCTACACATTTTTCCCACAAAGAAATGGCTGCAGGGTTACCCTTTACCAAGATGCTCATGTTCCAGACAATTTCATCCCCAACATCCCTTTAGCTGGAGGCAAGTGTTACGAACCTCATGGGTGTTGGGAAGATATTTTTCATGCAATCTATAATGCAAAGCACTTGATATACATTGCTGGTTGGTCTGTATTTACTAAAATCACATTAGTAAGGGACACAATGAGGCCAAAGATAGTAGACGGAGACTTGACTCTGGGAGAGCTTCTTAAGATGAAAGCAAAGGAAGGAGTGAAGGTCTTAATGCTTGTATGGGATGACAGAACTTCAGTGAAGGTACTAAAGAAAGATGGACTGATGGCTACTCATGATGAAGATACCGAAAGTTACTTCCATGACAGCGGAGTTCACTGCGTGTTGTGTCCCAGAAACCCTGACAATGGGCAAAGCATTGTTCAAGATATAGAGGTTGGAACAATGTTCACTCATCATCAAAAGATTGTGGTAGTGGACAGTGAATTGCCGAATGGAGCATTAGAAAAGAGGAGGATTGTGAGCTTCATTGGCGGCATTGATCTTTGTGATGGAAGATATGACACTCAGTTTCATCCCCTTTTCAGGACTTTAGGCACAATCCACCATGATGATCTCCATCAACCCAATTTTAATGGTGCCTCAATCGCAAAAGGTGGACCAAGGGAGCCTTGGCATGACATACATTGCAGGCTAGAAGGGCCAATTGCTTGGGATGTTTTATTCAACTTTGAGCAGAGGTGGAGAAAGCAAGGTGTGAAAGACTTACTTTTTCAACTCAGAGATTTTGATGAGACCTTTATACCACCATCTCCGGTTATGTATCCTGAAGACCATGACACATGGAATGTTCAGTTGTTCCGATCCATTGATGGAGGAGCTGCATTCGGATTTCCCAACTCTCCTGAAGATGCTACCAGGGCTGGTCTAGTCAGTGGCAGAGATCATGTGATTGACAGAAGCATTCAGGATGCATATATCAATGCAATTCGTCGAGCAAAGAGTTTTATCTACATCGAAAATCAGTACTTTCTTGGAAGCTCTTTTGGCTGGCAGTCAGATCATAAAGACCTCAAAGTTGAGGAGGTTGGCGCTTTGCATCTTATTCCAAAAGAACTGTCACTAAAGATTGTTAGTAAGATTGAAGCTGGAGAAAGATTTACTGTCTACATTGTCATGCCTATGTGGCCAGAGGGTGTTCCAGAAAGCCAATCTGTTCAGGCAATATTGCTTTGGCAGAAGTTGACAATGGAGATGATGTATAGGGACATAACAGAAGCTCTAAAGACCAAGGGGCTTGATGCAAACCCTAAGGAGTATTTGACATTCTTTTGCCTTGGAAATCGTGAGAAGAAGCGAAGCGGAGAATATGAACCTCATGAGAAACCACAGCATGATACAGATTACAGTAGAGCTCAACAATCTAGGCGGTTCATGATCTACGTACATGCCAAGTGTATGATAGTTGATGATGAATACATAATCATTGGATCCGCCAACATAAATCAGAGATCAATGGACGGCGCCAGGGATACCGAGATTGCCATGGGAGCTTACCAACCATATCACCTATCTACCAATGTACCTGCCCGGGGCCAAATCCATGGCCTCAGAATGGCATTGTGGTATGAACATTTGGGGTTGCTCAATAACACCTTTCTTGAGCCGGAGAGTGTGGAATGTGTGAGAATGGTGAACCAGATTGCCGAAAAGCATTGGGAACTCTATTTAAGTGAGACCTTAGAGGGTGACTTGCCAGGACATTTGCTACCCTATCCCGTAAAAATTACTGAAAATGGAGAGGTCACTGAGTTACCTGGGGTTGAATTTTTTCCAGACACCAAGGCTAAAGTTGATGGAACCAGATCTGACTTAATTCCTTCAATCATCACTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

809

Amino Acids

92.09

Weight (kDa)

6.12

Isoelectric Point (pI)

37.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	41 - 123	2.2e-10	C2 domain
PLDc	PF00614	326 - 363	1.2e-06	Phospholipase D Active site motif
PLDc_2	PF13091	655 - 691	1e-06	PLD-like domain
PLDc	PF00614	657 - 682	6.9e-07	Phospholipase D Active site motif
PLD_C	PF12357	728 - 798	7.3e-29	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25370 AT5G25370 AT5G25370 AT5G25370
fragaria_vesca	FvH4_1g03170
malus_domestica	MD02G1030700.v1.1 MD15G1172300.v1.1
prunus_persica	Prupe.7G243800_v2.0.a1
pyrus_communis	pycom02g02560 pycom15g15370
rosa_chinensis	RchiOBHm_Chr2g0088301
rosa_laevigata	RLG00000015940
rosa_multiflora	Rmu_sc0004235.1_g000036
rosa_roxburghii	Rroxscaffold_2G00152760
rosa_rugosa	Rorug01G0482700
rosa_samantha	Rh2AG037800 Rh2BG037000 Rh2CG038100 Rh2DG037500
rosa_wichuraiana	Rw2G002980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2125
AccB1I	GGYRCC	2 cut(s)	1169, 2054
AccI	GTMKAC	4 cut(s)	354, 391, 738, 1698
AciI	CCGC	5 cut(s)	900, 1065, 1893, 1960, 2025
AclWI	GGATC	4 cut(s)	387, 1389, 2016, 2029
AcoI	YGGCCR	1 cut(s)	1718
AcsI	RAATTY	2 cut(s)	485, 2357
AcuI	CTGAAG	3 cut(s)	810, 1380, 1458
AcyI	GRCGYC	1 cut(s)	2055
AdeI	CACNNNGTG	1 cut(s)	913
AfaI	GTAC	6 cut(s)	30, 498, 837, 1559, 1977, 2115
AfiI	CCNNNNNNNGG	6 cut(s)	401, 593, 938, 1116, 2123, 2146
AflII	CTTAAG	2 cut(s)	365, 767
AgsI	TTSAA	8 cut(s)	64, 104, 956, 1258, 1307, 1676, 2357, 2415
AjnI	CCWGG	5 cut(s)	490, 1448, 2057, 2287, 2347
AjuI	GAANNNNNNNTTGG	2 cut(s)	96, 128
Alw21I	GWGCWC	2 cut(s)	244, 1948
Alw26I	GTCTC	4 cut(s)	468, 738, 1319, 2261
AlwI	GGATC	4 cut(s)	387, 1389, 2016, 2029
Ama87I	CYCGRG	1 cut(s)	2121
AoxI	GGCC	6 cut(s)	294, 725, 1232, 1718, 2125, 2137
ApeKI	GCWGC	6 cut(s)	16, 19, 78, 326, 528, 1412
ApoI	RAATTY	2 cut(s)	485, 2357
ArsI	GACNNNNNNTTYG	4 cut(s)	136, 168, 1634, 1666
Asp700I	GAANNNNTTC	2 cut(s)	225, 2361
AspLEI	GCGC	3 cut(s)	7, 1625, 2057
AspS9I	GGNCC	4 cut(s)	294, 1190, 1232, 2125
AsuC2I	CCSGG	2 cut(s)	2122, 2123
AsuHPI	GGTGA	3 cut(s)	2090, 2236, 2291
AsuII	TTCGAA	1 cut(s)	48
AvaI	CYCGRG	1 cut(s)	2121
AvaII	GGWCC	1 cut(s)	1190
AxyI	CCTNAGG	1 cut(s)	1842
BaeI	ACNNNNGTAYC	2 cut(s)	2097, 2130
BalI	TGGCCA	1 cut(s)	1720
BamHI	GGATCC	1 cut(s)	2021
BanI	GGYRCC	2 cut(s)	1169, 2054
BanII	GRGCYC	1 cut(s)	1948
BbsI	GAAGAC	1 cut(s)	1368
Bbv12I	GWGCWC	2 cut(s)	244, 1948
BbvI	GCAGC	6 cut(s)	3, 6, 65, 313, 515, 1399
BccI	CCATC	9 cut(s)	278, 354, 842, 850, 1076, 1158, 1348, 1397, 2381
BceAI	ACGGC	1 cut(s)	2068
BciT130I	CCWGG	5 cut(s)	492, 1450, 2059, 2289, 2349
BciVI	GTATCC	2 cut(s)	1365, 2056
BcnI	CCSGG	2 cut(s)	2122, 2123
BcoDI	GTCTC	4 cut(s)	468, 738, 1319, 2261
BfaI	CTAG	4 cut(s)	180, 1226, 1460, 1956
BfmI	CTRYAG	1 cut(s)	529
BfoI	RGCGCY	2 cut(s)	1626, 2058
BfrI	CTTAAG	2 cut(s)	365, 767
BfuAI	ACCTGC	1 cut(s)	2125
BfuI	GTATCC	2 cut(s)	1365, 2056
BglI	GCCNNNNNGGC	1 cut(s)	1717
BglII	AGATCT	1 cut(s)	2396
BisI	GCNGC	7 cut(s)	17, 20, 79, 327, 529, 1066, 1413
BlsI	GCNGC	7 cut(s)	18, 21, 80, 328, 530, 1067, 1414
BmcAI	AGTACT	2 cut(s)	30, 1559
Bme1390I	CCNGG	7 cut(s)	492, 1450, 2059, 2122, 2123, 2289, 2349
Bme18I	GGWCC	1 cut(s)	1190
BmeT110I	CYCGRG	1 cut(s)	2121
BmgT120I	GGNCC	4 cut(s)	294, 1190, 1232, 2125
BmiI	GGNNCC	8 cut(s)	295, 409, 1171, 1200, 2023, 2056, 2126, 2392
BmrFI	CCNGG	7 cut(s)	492, 1450, 2059, 2122, 2123, 2289, 2349
BmsI	GCATC	5 cut(s)	541, 1432, 1492, 1639, 1822
BpiI	GAAGAC	1 cut(s)	1368
BpmI	CTGGAG	2 cut(s)	615, 1701
Bpu14I	TTCGAA	1 cut(s)	48
BpuEI	CTTGAG	1 cut(s)	2213
BpuMI	CCSGG	2 cut(s)	2122, 2123
BsaAI	YACGTR	1 cut(s)	1975
BsaBI	GATNNNNATC	1 cut(s)	1149
BsaHI	GRCGYC	1 cut(s)	2055
BsaI	GGTCTC	3 cut(s)	468, 1319, 2261
BsaWI	WCCGGW	1 cut(s)	1345
BsaXI	ACNNNNNCTCC	6 cut(s)	336, 366, 780, 810, 1775, 1805
Bsc4I	CCNNNNNNNGG	6 cut(s)	401, 593, 938, 1116, 2123, 2146
Bse21I	CCTNAGG	1 cut(s)	1842
Bse3DI	GCAATG	2 cut(s)	672, 1216
Bse8I	GATNNNNATC	1 cut(s)	1149
BseBI	CCWGG	5 cut(s)	492, 1450, 2059, 2289, 2349
BseGI	GGATG	7 cut(s)	436, 573, 582, 820, 1105, 1252, 1507
BseJI	GATNNNNATC	1 cut(s)	1149
BseLI	CCNNNNNNNGG	6 cut(s)	401, 593, 938, 1116, 2123, 2146
BseMI	GCAATG	2 cut(s)	672, 1216
BseMII	CTCAG	5 cut(s)	419, 1112, 1324, 2155, 2331
BseRI	GAGGAG	4 cut(s)	326, 1057, 1422, 1628
BseXI	GCAGC	6 cut(s)	3, 6, 65, 313, 515, 1399
BsgI	GTGCAG	1 cut(s)	64
BshFI	GGCC	6 cut(s)	296, 727, 1234, 1720, 2127, 2139
BshNI	GGYRCC	2 cut(s)	1169, 2054
BsiHKAI	GWGCWC	2 cut(s)	244, 1948
BsiHKCI	CYCGRG	1 cut(s)	2121
BsiSI	CCGG	3 cut(s)	1346, 2122, 2198
BslFI	GGGAC	3 cut(s)	728, 906, 1811
BslI	CCNNNNNNNGG	6 cut(s)	401, 593, 938, 1116, 2123, 2146
BsmAI	GTCTC	4 cut(s)	468, 738, 1319, 2261
BsmFI	GGGAC	3 cut(s)	728, 906, 1811
BsmI	GAATGC	2 cut(s)	1415, 1494
BsnI	GGCC	6 cut(s)	296, 727, 1234, 1720, 2127, 2139
Bso31I	GGTCTC	3 cut(s)	468, 1319, 2261
BsoBI	CYCGRG	1 cut(s)	2121
Bsp119I	TTCGAA	1 cut(s)	48
Bsp1286I	GDGCHC	2 cut(s)	244, 1948
Bsp19I	CCATGG	2 cut(s)	2076, 2134
BspACI	CCGC	5 cut(s)	900, 1065, 1893, 1960, 2025
BspANI	GGCC	6 cut(s)	296, 727, 1234, 1720, 2127, 2139
BspCNI	CTCAG	5 cut(s)	420, 1111, 1323, 2154, 2332
BspHI	TCATGA	3 cut(s)	865, 1909, 1965
BspLI	GGNNCC	8 cut(s)	295, 409, 1171, 1200, 2023, 2056, 2126, 2392
BspMAI	CTGCAG	1 cut(s)	533
BspMI	ACCTGC	1 cut(s)	2125
BspPI	GGATC	4 cut(s)	387, 1389, 2016, 2029
BspQI	GCTCTTC	1 cut(s)	304
BspT104I	TTCGAA	1 cut(s)	48
BspT107I	GGYRCC	2 cut(s)	1169, 2054
BspTI	CTTAAG	2 cut(s)	365, 767
BspTNI	GGTCTC	3 cut(s)	468, 1319, 2261
BsrDI	GCAATG	2 cut(s)	672, 1216
BssNI	GRCGYC	1 cut(s)	2055
BssT1I	CCWWGG	7 cut(s)	1193, 1202, 1821, 1867, 2076, 2134, 2373
Bst2UI	CCWGG	5 cut(s)	492, 1450, 2059, 2289, 2349
Bst4CI	ACNGT	9 cut(s)	13, 213, 239, 389, 455, 1013, 1650, 1696, 1940
Bst6I	CTCTTC	2 cut(s)	99, 304
BstACI	GRCGYC	1 cut(s)	2055
BstAFI	CTTAAG	2 cut(s)	365, 767
BstAPI	GCANNNNNTGC	1 cut(s)	13
BstBAI	YACGTR	1 cut(s)	1975
BstBI	TTCGAA	1 cut(s)	48
BstC8I	GCNNGC	4 cut(s)	95, 324, 1223, 1583
BstDEI	CTNAG	7 cut(s)	428, 1098, 1310, 1842, 2141, 2272, 2340
BstDSI	CCRYGG	2 cut(s)	2076, 2134
BstEII	GGTNACC	1 cut(s)	535
BstF5I	GGATG	7 cut(s)	436, 573, 582, 820, 1105, 1252, 1507
BstH2I	RGCGCY	2 cut(s)	1626, 2058
BstHHI	GCGC	3 cut(s)	7, 1625, 2057
BstMAI	GTCTC	4 cut(s)	468, 738, 1319, 2261
BstMWI	GCNNNNNNNGC	9 cut(s)	13, 248, 1062, 1178, 1231, 1451, 1578, 1717, 2081
BstNI	CCWGG	5 cut(s)	492, 1450, 2059, 2289, 2349
BstNSI	RCATGY	1 cut(s)	1982
BstPI	GGTNACC	1 cut(s)	535
BstSCI	CCNGG	7 cut(s)	490, 1448, 2057, 2120, 2121, 2287, 2347
BstSFI	CTRYAG	1 cut(s)	529
BstSNI	TACGTA	1 cut(s)	1975
BstV1I	GCAGC	6 cut(s)	3, 6, 65, 313, 515, 1399
BstV2I	GAAGAC	1 cut(s)	1368
BstX2I	RGATCY	2 cut(s)	2021, 2396
BstXI	CCANNNNNNTGG	3 cut(s)	154, 1242, 2135
BstYI	RGATCY	2 cut(s)	2021, 2396
Bsu36I	CCTNAGG	1 cut(s)	1842
BsuI	GTATCC	2 cut(s)	1365, 2056
BsuRI	GGCC	6 cut(s)	296, 727, 1234, 1720, 2127, 2139
BtgI	CCRYGG	2 cut(s)	2076, 2134
BtsCI	GGATG	7 cut(s)	436, 573, 582, 820, 1105, 1252, 1507
BtsI	GCAGTG	1 cut(s)	907
BtsIMutI	CAGTG	6 cut(s)	218, 834, 907, 1018, 1471, 2337
BveI	ACCTGC	1 cut(s)	2125
Cac8I	GCNNGC	4 cut(s)	95, 324, 1223, 1583
CciI	TCATGA	3 cut(s)	865, 1909, 1965
CfoI	GCGC	3 cut(s)	7, 1625, 2057
Cfr13I	GGNCC	4 cut(s)	294, 1190, 1232, 2125
Cfr9I	CCCGGG	1 cut(s)	2121
Csp6I	GTAC	6 cut(s)	29, 497, 836, 1558, 1976, 2114
CviQI	GTAC	6 cut(s)	29, 497, 836, 1558, 1976, 2114
DdeI	CTNAG	7 cut(s)	428, 1098, 1310, 1842, 2141, 2272, 2340
DinI	GGCGCC	1 cut(s)	2056
DraIII	CACNNNGTG	1 cut(s)	913
EaeI	YGGCCR	1 cut(s)	1718
Eam1104I	CTCTTC	2 cut(s)	99, 304
EarI	CTCTTC	2 cut(s)	99, 304
EciI	GGCGGA	1 cut(s)	2014
Ecl136II	GAGCTC	1 cut(s)	1946
Eco105I	TACGTA	1 cut(s)	1975
Eco130I	CCWWGG	7 cut(s)	1193, 1202, 1821, 1867, 2076, 2134, 2373
Eco24I	GRGCYC	1 cut(s)	1948
Eco31I	GGTCTC	3 cut(s)	468, 1319, 2261
Eco47I	GGWCC	1 cut(s)	1190
Eco53kI	GAGCTC	1 cut(s)	1946
Eco57I	CTGAAG	3 cut(s)	810, 1380, 1458
Eco81I	CCTNAGG	1 cut(s)	1842
Eco88I	CYCGRG	1 cut(s)	2121
Eco91I	GGTNACC	1 cut(s)	535
EcoICRI	GAGCTC	1 cut(s)	1946
EcoO65I	GGTNACC	1 cut(s)	535
EcoRII	CCWGG	5 cut(s)	490, 1448, 2057, 2287, 2347
EcoT14I	CCWWGG	7 cut(s)	1193, 1202, 1821, 1867, 2076, 2134, 2373
EcoT22I	ATGCAT	1 cut(s)	1507
EcoT38I	GRGCYC	1 cut(s)	1948
EgeI	GGCGCC	1 cut(s)	2056
EheI	GGCGCC	1 cut(s)	2056
ErhI	CCWWGG	7 cut(s)	1193, 1202, 1821, 1867, 2076, 2134, 2373
FalI	AAGNNNNNCTT	4 cut(s)	1186, 1218, 1284, 1316
FaqI	GGGAC	3 cut(s)	728, 906, 1811
FauNDI	CATATG	2 cut(s)	89, 246
FblI	GTMKAC	4 cut(s)	354, 391, 738, 1698
Fnu4HI	GCNGC	7 cut(s)	17, 20, 79, 327, 529, 1066, 1413
FokI	GGATG	7 cut(s)	423, 560, 569, 827, 1092, 1259, 1514
FriOI	GRGCYC	1 cut(s)	1948
Fsp4HI	GCNGC	7 cut(s)	17, 20, 79, 327, 529, 1066, 1413
FspBI	CTAG	4 cut(s)	180, 1226, 1460, 1956
GlaI	GCGC	3 cut(s)	6, 1624, 2056
GluI	GCNGC	7 cut(s)	17, 20, 79, 327, 529, 1066, 1413
GsuI	CTGGAG	2 cut(s)	615, 1701
HaeII	RGCGCY	2 cut(s)	1626, 2058
HaeIII	GGCC	6 cut(s)	296, 727, 1234, 1720, 2127, 2139
HapII	CCGG	3 cut(s)	1346, 2122, 2198
HhaI	GCGC	3 cut(s)	7, 1625, 2057
Hin1I	GRCGYC	1 cut(s)	2055
Hin6I	GCGC	3 cut(s)	5, 1623, 2055
HinP1I	GCGC	3 cut(s)	5, 1623, 2055
HincII	GTYRAC	1 cut(s)	1776
HindII	GTYRAC	1 cut(s)	1776
HinfI	GANTC	4 cut(s)	107, 221, 471, 751
HpaII	CCGG	3 cut(s)	1346, 2122, 2198
HphI	GGTGA	3 cut(s)	2090, 2236, 2291
Hpy188I	TCNGA	9 cut(s)	308, 374, 1313, 1394, 1421, 1591, 2040, 2144, 2401
Hpy99I	CGWCG	1 cut(s)	1527
HpyAV	CCTTC	7 cut(s)	58, 94, 779, 787, 826, 1223, 2421
HpyCH4III	ACNGT	9 cut(s)	13, 213, 239, 389, 455, 1013, 1650, 1696, 1940
HpyCH4IV	ACGT	1 cut(s)	1974
HpyF10VI	GCNNNNNNNGC	9 cut(s)	13, 248, 1062, 1178, 1231, 1451, 1578, 1717, 2081
HpyF3I	CTNAG	7 cut(s)	428, 1098, 1310, 1842, 2141, 2272, 2340
HpySE526I	ACGT	1 cut(s)	1974
Hsp92I	GRCGYC	1 cut(s)	2055
HspAI	GCGC	3 cut(s)	5, 1623, 2055
KasI	GGCGCC	1 cut(s)	2054
LguI	GCTCTTC	1 cut(s)	304
LmnI	GCTCC	4 cut(s)	1028, 1198, 1409, 2081
Lsp1109I	GCAGC	6 cut(s)	3, 6, 65, 313, 515, 1399
LweI	GCATC	5 cut(s)	541, 1432, 1492, 1639, 1822
MaeI	CTAG	4 cut(s)	180, 1226, 1460, 1956
MaeII	ACGT	1 cut(s)	1974
MaeIII	GTNAC	8 cut(s)	442, 535, 605, 884, 1650, 2279, 2335, 2343
MfeI	CAATTG	1 cut(s)	1236
MflI	RGATCY	2 cut(s)	2021, 2396
MhlI	GDGCHC	2 cut(s)	244, 1948
MlsI	TGGCCA	1 cut(s)	1720
MluNI	TGGCCA	1 cut(s)	1720
Mly113I	GGCGCC	1 cut(s)	2055
MlyI	GAGTC	2 cut(s)	116, 745
MmeI	TCCRAC	1 cut(s)	949
Mox20I	TGGCCA	1 cut(s)	1720
Mph1103I	ATGCAT	1 cut(s)	1507
MroXI	GAANNNNTTC	2 cut(s)	225, 2361
MscI	TGGCCA	1 cut(s)	1720
MseI	TTAA	7 cut(s)	366, 768, 800, 1164, 2261, 2405, 2428
MslI	CAYNNNNRTG	3 cut(s)	1140, 1706, 1712
Msp20I	TGGCCA	1 cut(s)	1720
MspA1I	CMGCKG	1 cut(s)	900
MspCI	CTTAAG	2 cut(s)	365, 767
MspI	CCGG	3 cut(s)	1346, 2122, 2198
MspR9I	CCNGG	7 cut(s)	492, 1450, 2059, 2122, 2123, 2289, 2349
MunI	CAATTG	1 cut(s)	1236
Mva1269I	GAATGC	2 cut(s)	1415, 1494
MvaI	CCWGG	5 cut(s)	492, 1450, 2059, 2289, 2349
MwoI	GCNNNNNNNGC	9 cut(s)	13, 248, 1062, 1178, 1231, 1451, 1578, 1717, 2081
NarI	GGCGCC	1 cut(s)	2055
NciI	CCSGG	2 cut(s)	2122, 2123
NcoI	CCATGG	2 cut(s)	2076, 2134
NdeI	CATATG	2 cut(s)	89, 246
NlaIV	GGNNCC	8 cut(s)	295, 409, 1171, 1200, 2023, 2056, 2126, 2392
NmuCI	GTSAC	3 cut(s)	1650, 2279, 2335
NsiI	ATGCAT	1 cut(s)	1507
NspI	RCATGY	1 cut(s)	1982
NspV	TTCGAA	1 cut(s)	48
PagI	TCATGA	3 cut(s)	865, 1909, 1965
PciSI	GCTCTTC	1 cut(s)	304
PctI	GAATGC	2 cut(s)	1415, 1494
PdmI	GAANNNNTTC	2 cut(s)	225, 2361
PfeI	GAWTC	2 cut(s)	221, 471
PfoI	TCCNGGA	1 cut(s)	490
PkrI	GCNGC	7 cut(s)	18, 21, 80, 328, 530, 1067, 1414
PleI	GAGTC	2 cut(s)	115, 745
PluTI	GGCGCC	1 cut(s)	2058
PpsI	GAGTC	2 cut(s)	115, 745
Ppu21I	YACGTR	1 cut(s)	1975
Psp124BI	GAGCTC	1 cut(s)	1948
Psp6I	CCWGG	5 cut(s)	490, 1448, 2057, 2287, 2347
PspEI	GGTNACC	1 cut(s)	535
PspGI	CCWGG	5 cut(s)	490, 1448, 2057, 2287, 2347
PspN4I	GGNNCC	8 cut(s)	295, 409, 1171, 1200, 2023, 2056, 2126, 2392
PspPI	GGNCC	4 cut(s)	294, 1190, 1232, 2125
PstI	CTGCAG	1 cut(s)	533
PsuI	RGATCY	2 cut(s)	2021, 2396
RsaI	GTAC	6 cut(s)	30, 498, 837, 1559, 1977, 2115
RsaNI	GTAC	6 cut(s)	29, 497, 836, 1558, 1976, 2114
RseI	CAYNNNNRTG	3 cut(s)	1140, 1706, 1712
SacI	GAGCTC	1 cut(s)	1948
SapI	GCTCTTC	1 cut(s)	304
SaqAI	TTAA	7 cut(s)	366, 768, 800, 1164, 2261, 2405, 2428
SatI	GCNGC	7 cut(s)	17, 20, 79, 327, 529, 1066, 1413
Sau96I	GGNCC	4 cut(s)	294, 1190, 1232, 2125
ScaI	AGTACT	2 cut(s)	30, 1559
SchI	GAGTC	2 cut(s)	116, 745
ScrFI	CCNGG	7 cut(s)	492, 1450, 2059, 2122, 2123, 2289, 2349
SduI	GDGCHC	2 cut(s)	244, 1948
SfaNI	GCATC	5 cut(s)	541, 1432, 1492, 1639, 1822
SfcI	CTRYAG	1 cut(s)	529
SfoI	GGCGCC	1 cut(s)	2056
SfuI	TTCGAA	1 cut(s)	48
SinI	GGWCC	1 cut(s)	1190
SmaI	CCCGGG	1 cut(s)	2123
SmiMI	CAYNNNNRTG	3 cut(s)	1140, 1706, 1712
SmlI	CTYRAG	3 cut(s)	365, 767, 2192
SmoI	CTYRAG	3 cut(s)	365, 767, 2192
SnaBI	TACGTA	1 cut(s)	1975
SsiI	CCGC	5 cut(s)	900, 1065, 1893, 1960, 2025
SspDI	GGCGCC	1 cut(s)	2054
SspI	AATATT	2 cut(s)	45, 1758
SspMI	CTAG	4 cut(s)	180, 1226, 1460, 1956
SstI	GAGCTC	1 cut(s)	1948
StyD4I	CCNGG	7 cut(s)	490, 1448, 2057, 2120, 2121, 2287, 2347
StyI	CCWWGG	7 cut(s)	1193, 1202, 1821, 1867, 2076, 2134, 2373
TaaI	ACNGT	9 cut(s)	13, 213, 239, 389, 455, 1013, 1650, 1696, 1940
TaiI	ACGT	1 cut(s)	1977
TaqI	TCGA	4 cut(s)	48, 54, 1525, 1548
TatI	WGTACW	2 cut(s)	28, 1557
TauI	GCSGC	1 cut(s)	1068
TfiI	GAWTC	2 cut(s)	221, 471
Tru1I	TTAA	7 cut(s)	366, 768, 800, 1164, 2261, 2405, 2428
Tru9I	TTAA	7 cut(s)	366, 768, 800, 1164, 2261, 2405, 2428
TscAI	CASTG	6 cut(s)	218, 834, 914, 1018, 1471, 2344
TseFI	GTSAC	3 cut(s)	1650, 2279, 2335
TseI	GCWGC	6 cut(s)	16, 19, 78, 326, 528, 1412
Tsp45I	GTSAC	3 cut(s)	1650, 2279, 2335
TspGWI	ACGGA	1 cut(s)	756
TspMI	CCCGGG	1 cut(s)	2121
TspRI	CASTG	6 cut(s)	218, 834, 914, 1018, 1471, 2344
Vha464I	CTTAAG	2 cut(s)	365, 767
VpaK11BI	GGWCC	1 cut(s)	1190
XapI	RAATTY	2 cut(s)	485, 2357
XceI	RCATGY	1 cut(s)	1982
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1200
XmaI	CCCGGG	1 cut(s)	2121
XmiI	GTMKAC	4 cut(s)	354, 391, 738, 1698
XmnI	GAANNNNTTC	2 cut(s)	225, 2361
XspI	CTAG	4 cut(s)	180, 1226, 1460, 1956
ZrmI	AGTACT	2 cut(s)	30, 1559
Zsp2I	ATGCAT	1 cut(s)	1507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.