Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010738

Physical Location & Seq

Genomic Coordinates Reverse (-)

2397005 .. 2400284

Gene Structure 3280 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1030700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGTTTATTGGTTGTGTGATTTCGTCCTGCAAAGTGGCTGCAACTACAGTAACATTTACTGTATCTATGTCCATAAATGACTCACAAGTCGCATTTCTTGGAGAGTTAGTAGGGCTAGGGAAAGGTTCCGGACTCTATGCAACAATTGATTTGGAAAATGTAAGAGTTGGACGAACTAGACTGCTTGAAAACTCAACGAAAAATCCCCAGTGGGGTGAATCTTTTCATATTTACTGTGCACACATGACTTCAAATGTTGTTTTCTCCATCAAAGAAGACAAAGCTTTTGGGGCCAAAGTTATCGGAAGGGCTTACATGCCAGCAGCAGAACTCCTCGATGGCAAAGAAGTAGACAGATGGCTTAAGATCATGTATGACAACAACAAACCTCTGCATATACGTTCGAAAATTCATGTAAAGTTGCAGTTCTTTAACGTAAACCAGGACTTGAATTGGTCTTGTGGAATAAGAAGTCCTGAATTCCAAGGAGTTCCATACACATTTTTCACTCAGAAAAATGGTTGCAGAGTTACCCTTTACCAAAATGCTCATGTCCCCAAAAAATTCACCCCGAAAATTCCTCTTGCCGGTTCCATGTGTTATGACCAGCACCGATGCTGGGAAGACATTTTTCATGCCATTTCTAATGCAAAACACTTGATATACATAGCAGGTTGGTCTGTATATACTAAAATCACATTAGTAAGGGACCCGAACAGTCAAATCCCGGGCGGCGATTTGACCCTCGGAGAGCTTCTTAAGAAGAAGGCAGATGAAGGAGTGAAAGTCCTAATGCTTGTGTGGGATGACAGAACTTCGGTGAAGTTTCTGAAGAGAGATGGAGTGATGGCTACTCACGATGAAGACACTGAAAGTTACTTCCACAACACGGCAGTTCACTGCGTGTTGTGTCCCCGAAACCCTGACAACGGGAATAGCATTGTTGAGGATTTAGAGGTTTCCACCATGTTTACGCATCACCAGAAGATTGTTGTGGTGGACAGCGAATTGCCTAATGGAGAAGGGTTGGAAAAGAGGAGGATTGTGAGCTTCATTGGTGGCATTGATCTTTGTGATGGGAGATATGACACTCCTTCACATCCAATTTTCAGGACTTTAGACACAACCCACAAGAATGATTTTCATCAGCCTAATTTTGAGGGAGCGTCGATTGCAAAAGGCGGTCCAAGGGAGCCTTGGCACGACATACATTGTCGGTTGGAAGGGCCTATTGCATGGGACGTCTTGTTCAATTTCGAGCAAAGATGGAGGAAGCAAGGAGGGAAGGACTTGCTTGTTGAACTCACAGAGCTTGATGACACATTTATGCCGCCATCTCCAGTTATGTTCCCTCAAGACCATGAGACGTGGACTGTTCAGTTGTTTCGCTCCATCGATGGAGGAGCTGCATTCGGATTCCCTGACTCCCCCGAAGATGCTGCCAGGGCAGGCCTTGTCACCGGGAAAGATCACGTGATTGATCGAAGCATTCAGGATGCTTATATCAATGCCATTAGGCGCGCAAAGAGTTTTATCTACATTGAAAATCAGTACTTTCTTGGAAGCTCTTTCGGATGGCATTCAGATTATGATACCCTAAAAGTAGAGGAGGTTGGTGCTTTGCATCTGATACCAAAGGAACTGTCACTAAAGATTGTTAGTAAGATTGAAGCCGGAGAAAGATTTACGGTCTACATTGTGATGCCAATGTGGCCAGAGGGCATCCCGGAAAGCCAAACGGTTCAGGCAATATTGCATTGGCAGAGGATGACGATGGATATGATGTATAGGGACATCGTTCGAGCTCTCAAAGCCAAGGGGATTGAGGCAAACCCTAAGGATTATTTGGCTTTCTTTTGCCTTGGTAACCGCGAGAAGAAGCTACCCGGAGAATATGAACCTCCGGAAAAACCAGAACATGGTTCGGATTACAGTCGAGCGCAGCAAGCAAGGCGGTTCATGATCTATGTTCATGCCAAGTTGATGATAGTTGATGATGAGTACATAATCATTGGATCAGCCAACATCAACCAGAGATCAATGGATGGAGCCAGGGATTCGGAGATTGCGATGGGAGCCTACCAACCCTACCATCTATCCACTGGAAAGCCGGCCCGGGGACAAATTCATGGCCTCAGAATGTCGTTGTGGTATGAACACTTAGGGCTGCTTGATGACACCTTTCTTGAACCAGAAAATGTGGAATGCTTTAGAAAGGTGAACCAAATTGCAGACCAGCATTGGGACCTTTATTCATGTGACACATTAGATGGTGACTTGCCAGGGCATTTGCTTAGCTACCCCATTGCAGTTACTGAAGACGGAGAGGTCACGGAGTTGCCTGGAACAGAATTTTTTCCGGACACCAAGGCTCGAATTCTCGGGTCGAAATCTGATTTGCTTCCTTCAATACTCACTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

808

Amino Acids

91.36

Weight (kDa)

5.81

Isoelectric Point (pI)

36.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	43 - 122	1.6e-07	C2 domain
PLDc	PF00614	323 - 361	1.3e-06	Phospholipase D Active site motif
PLDc_2	PF13091	654 - 687	3.5e-06	PLD-like domain
PLDc	PF00614	655 - 680	1.1e-07	Phospholipase D Active site motif
PLD_C	PF12357	725 - 797	9.6e-29	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25370 AT5G25370 AT5G25370 AT5G25370
fragaria_vesca	FvH4_1g03170
malus_domestica	MD02G1030700.v1.1 MD15G1172300.v1.1
prunus_persica	Prupe.7G243800_v2.0.a1
pyrus_communis	pycom02g02560 pycom15g15370
rosa_chinensis	RchiOBHm_Chr2g0088301
rosa_laevigata	RLG00000015940
rosa_multiflora	Rmu_sc0004235.1_g000036
rosa_roxburghii	Rroxscaffold_2G00152760
rosa_rugosa	Rorug01G0482700
rosa_samantha	Rh2AG037800 Rh2BG037000 Rh2CG038100 Rh2DG037500
rosa_wichuraiana	Rw2G002980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	86, 2327
AatII	GACGTC	1 cut(s)	1245
Acc36I	ACCTGC	1 cut(s)	662
AccB7I	CCANNNNNTGG	1 cut(s)	1919
AccI	GTMKAC	2 cut(s)	351, 1692
AccII	CGCG	2 cut(s)	1521, 1872
AccIII	TCCGGA	3 cut(s)	128, 1903, 2359
AciI	CCGC	5 cut(s)	732, 1182, 1331, 1870, 1954
AclWI	GGATC	1 cut(s)	2023
AcoI	YGGCCR	1 cut(s)	1712
AcsI	RAATTY	7 cut(s)	408, 479, 563, 576, 2124, 2351, 2376
AcuI	CTGAAG	2 cut(s)	851, 2337
AcvI	CACGTG	1 cut(s)	1474
AcyI	GRCGYC	1 cut(s)	1242
AdeI	CACNNNGTG	1 cut(s)	904
AfaI	GTAC	2 cut(s)	1553, 2003
AfiI	CCNNNNNNNGG	8 cut(s)	212, 587, 619, 889, 929, 1919, 2117, 2242
AflII	CTTAAG	2 cut(s)	362, 758
AgsI	TTSAA	9 cut(s)	188, 252, 451, 1252, 1301, 1544, 1670, 2189, 2409
AjiI	CACGTC	1 cut(s)	1368
AjnI	CCWGG	5 cut(s)	441, 1442, 2051, 2281, 2341
AloI	GAACNNNNNNTCC	2 cut(s)	1232, 1264
AluBI	AGCT	9 cut(s)	284, 754, 1050, 1312, 1406, 1566, 1805, 1882, 2298
AluI	AGCT	9 cut(s)	284, 754, 1050, 1312, 1406, 1566, 1805, 1882, 2298
Alw21I	GWGCWC	2 cut(s)	241, 1807
Alw26I	GTCTC	1 cut(s)	1358
Alw44I	GTGCAC	1 cut(s)	237
AlwI	GGATC	1 cut(s)	2023
AlwNI	CAGNNNCTG	1 cut(s)	2315
Ama87I	CYCGRG	3 cut(s)	727, 2115, 2381
Aor13HI	TCCGGA	3 cut(s)	128, 1903, 2359
AoxI	GGCC	6 cut(s)	291, 1226, 1450, 1712, 2112, 2131
ApaLI	GTGCAC	1 cut(s)	237
ApeKI	GCWGC	6 cut(s)	38, 323, 1406, 1439, 1942, 2167
ApoI	RAATTY	7 cut(s)	408, 479, 563, 576, 2124, 2351, 2376
ArsI	GACNNNNNNTTYG	4 cut(s)	269, 301, 1628, 1660
Asp700I	GAANNNNTTC	2 cut(s)	222, 2355
AspLEI	GCGC	3 cut(s)	1521, 1523, 1942
AspS9I	GGNCC	6 cut(s)	291, 709, 1184, 1226, 2113, 2245
AsuC2I	CCSGG	7 cut(s)	728, 729, 1462, 1727, 1887, 2116, 2117
AsuHPI	GGTGA	7 cut(s)	227, 559, 832, 971, 1450, 2230, 2285
AsuII	TTCGAA	1 cut(s)	404
AvaI	CYCGRG	3 cut(s)	727, 2115, 2381
AvaII	GGWCC	3 cut(s)	709, 1184, 2245
AxyI	CCTNAGG	1 cut(s)	1836
BaeGI	GKGCMC	1 cut(s)	241
BalI	TGGCCA	1 cut(s)	1714
BanII	GRGCYC	1 cut(s)	1807
BbrPI	CACGTG	1 cut(s)	1474
BbsI	GAAGAC	4 cut(s)	282, 630, 870, 2325
Bbv12I	GWGCWC	2 cut(s)	241, 1807
BbvI	GCAGC	6 cut(s)	25, 335, 1393, 1426, 1954, 2154
BceAI	ACGGC	1 cut(s)	906
BcgI	CGANNNNNNTGC	2 cut(s)	1421, 1455
BciT130I	CCWGG	5 cut(s)	443, 1444, 2053, 2283, 2343
BcnI	CCSGG	7 cut(s)	728, 729, 1462, 1727, 1887, 2116, 2117
BcoDI	GTCTC	1 cut(s)	1358
BfaI	CTAG	2 cut(s)	116, 177
BfmI	CTRYAG	1 cut(s)	45
BfrI	CTTAAG	2 cut(s)	362, 758
BfuAI	ACCTGC	1 cut(s)	662
BglI	GCCNNNNNGGC	1 cut(s)	1711
BisI	GCNGC	8 cut(s)	39, 324, 733, 1331, 1407, 1440, 1943, 2168
BlpI	GCTNAGC	1 cut(s)	2294
BlsI	GCNGC	8 cut(s)	40, 325, 734, 1332, 1408, 1441, 1944, 2169
BmcAI	AGTACT	1 cut(s)	1553
Bme18I	GGWCC	3 cut(s)	709, 1184, 2245
BmeT110I	CYCGRG	3 cut(s)	727, 2115, 2381
BmgBI	CACGTC	1 cut(s)	1368
BmgT120I	GGNCC	6 cut(s)	291, 709, 1184, 1226, 2113, 2245
BmiI	GGNNCC	9 cut(s)	127, 292, 592, 710, 711, 1194, 2050, 2077, 2246
BmrI	ACTGGG	1 cut(s)	202
BmsI	GCATC	7 cut(s)	605, 985, 1426, 1486, 1633, 1692, 1731
BmuI	ACTGGG	1 cut(s)	202
BpiI	GAAGAC	4 cut(s)	282, 630, 870, 2325
BpmI	CTGGAG	1 cut(s)	1323
Bpu1102I	GCTNAGC	1 cut(s)	2294
Bpu14I	TTCGAA	1 cut(s)	404
BpuEI	CTTGAG	1 cut(s)	1338
BpuMI	CCSGG	7 cut(s)	728, 729, 1462, 1727, 1887, 2116, 2117
Bsa29I	ATCGAT	1 cut(s)	1395
BsaAI	YACGTR	1 cut(s)	1474
BsaBI	GATNNNNATC	1 cut(s)	1143
BsaHI	GRCGYC	1 cut(s)	1242
BsaWI	WCCGGW	3 cut(s)	128, 1903, 2359
BsaXI	ACNNNNNCTCC	4 cut(s)	93, 123, 771, 801
Bsc4I	CCNNNNNNNGG	8 cut(s)	212, 587, 619, 889, 929, 1919, 2117, 2242
Bse118I	RCCGGY	2 cut(s)	587, 2110
Bse1I	ACTGG	3 cut(s)	208, 1340, 2107
Bse21I	CCTNAGG	1 cut(s)	1836
Bse3DI	GCAATG	1 cut(s)	2304
Bse8I	GATNNNNATC	1 cut(s)	1143
BseAI	TCCGGA	3 cut(s)	128, 1903, 2359
BseBI	CCWGG	5 cut(s)	443, 1444, 2053, 2283, 2343
BseCI	ATCGAT	1 cut(s)	1395
BseGI	GGATG	7 cut(s)	811, 1099, 1501, 1580, 1722, 1773, 2050
BseJI	GATNNNNATC	1 cut(s)	1143
BseLI	CCNNNNNNNGG	8 cut(s)	212, 587, 619, 889, 929, 1919, 2117, 2242
BseMI	GCAATG	1 cut(s)	2304
BseMII	CTCAG	2 cut(s)	524, 2149
BseNI	ACTGG	3 cut(s)	208, 1340, 2107
BsePI	GCGCGC	1 cut(s)	1519
BseRI	GAGGAG	4 cut(s)	323, 1051, 1416, 1622
BseSI	GKGCMC	1 cut(s)	241
BseXI	GCAGC	6 cut(s)	25, 335, 1393, 1426, 1954, 2154
BseYI	CCCAGC	1 cut(s)	618
Bsh1236I	CGCG	2 cut(s)	1521, 1872
BshFI	GGCC	6 cut(s)	293, 1228, 1452, 1714, 2114, 2133
BshVI	ATCGAT	1 cut(s)	1395
BsiHKAI	GWGCWC	2 cut(s)	241, 1807
BsiHKCI	CYCGRG	3 cut(s)	727, 2115, 2381
BslFI	GGGAC	7 cut(s)	539, 722, 897, 1253, 1805, 2133, 2258
BslI	CCNNNNNNNGG	8 cut(s)	212, 587, 619, 889, 929, 1919, 2117, 2242
BsmAI	GTCTC	1 cut(s)	1358
BsmBI	CGTCTC	1 cut(s)	1358
BsmFI	GGGAC	7 cut(s)	539, 722, 897, 1253, 1805, 2133, 2258
BsmI	GAATGC	4 cut(s)	1409, 1488, 1579, 2210
BsnI	GGCC	6 cut(s)	293, 1228, 1452, 1714, 2114, 2133
BsoBI	CYCGRG	3 cut(s)	727, 2115, 2381
Bsp119I	TTCGAA	1 cut(s)	404
Bsp1286I	GDGCHC	2 cut(s)	241, 1807
Bsp13I	TCCGGA	3 cut(s)	128, 1903, 2359
Bsp143I	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
Bsp1720I	GCTNAGC	1 cut(s)	2294
BspACI	CCGC	5 cut(s)	732, 1182, 1331, 1870, 1954
BspANI	GGCC	6 cut(s)	293, 1228, 1452, 1714, 2114, 2133
BspCNI	CTCAG	2 cut(s)	523, 2148
BspDI	ATCGAT	1 cut(s)	1395
BspEI	TCCGGA	3 cut(s)	128, 1903, 2359
BspFNI	CGCG	2 cut(s)	1521, 1872
BspHI	TCATGA	1 cut(s)	1959
BspLI	GGNNCC	9 cut(s)	127, 292, 592, 710, 711, 1194, 2050, 2077, 2246
BspMI	ACCTGC	1 cut(s)	662
BspPI	GGATC	1 cut(s)	2023
BspT104I	TTCGAA	1 cut(s)	404
BspTI	CTTAAG	2 cut(s)	362, 758
BsrDI	GCAATG	1 cut(s)	2304
BsrFI	RCCGGY	2 cut(s)	587, 2110
BsrI	ACTGG	3 cut(s)	208, 1340, 2107
BssAI	RCCGGY	2 cut(s)	587, 2110
BssHII	GCGCGC	1 cut(s)	1519
BssMI	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
BssNI	GRCGYC	1 cut(s)	1242
BssT1I	CCWWGG	6 cut(s)	484, 1187, 1196, 1815, 1861, 2367
Bst2UI	CCWGG	5 cut(s)	443, 1444, 2053, 2283, 2343
Bst4CI	ACNGT	9 cut(s)	49, 61, 236, 719, 1375, 1644, 1690, 1741, 1934
Bst6I	CTCTTC	1 cut(s)	827
BstACI	GRCGYC	1 cut(s)	1242
BstAFI	CTTAAG	2 cut(s)	362, 758
BstBAI	YACGTR	1 cut(s)	1474
BstBI	TTCGAA	1 cut(s)	404
BstC8I	GCNNGC	5 cut(s)	321, 1450, 1521, 1947, 2112
BstDEI	CTNAG	6 cut(s)	510, 1836, 2135, 2161, 2294, 2421
BstEII	GGTNACC	1 cut(s)	1865
BstF5I	GGATG	7 cut(s)	811, 1099, 1501, 1580, 1722, 1773, 2050
BstFNI	CGCG	2 cut(s)	1521, 1872
BstHHI	GCGC	3 cut(s)	1521, 1523, 1942
BstKTI	GATC	7 cut(s)	369, 1069, 1471, 1483, 1965, 2018, 2039
BstMAI	GTCTC	1 cut(s)	1358
BstMBI	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
BstMWI	GCNNNNNNNGC	8 cut(s)	290, 1172, 1445, 1711, 1811, 1946, 1951, 2075
BstNI	CCWGG	5 cut(s)	443, 1444, 2053, 2283, 2343
BstNSI	RCATGY	1 cut(s)	319
BstPI	GGTNACC	1 cut(s)	1865
BstSFI	CTRYAG	1 cut(s)	45
BstSLI	GKGCMC	1 cut(s)	241
BstUI	CGCG	2 cut(s)	1521, 1872
BstV1I	GCAGC	6 cut(s)	25, 335, 1393, 1426, 1954, 2154
BstV2I	GAAGAC	4 cut(s)	282, 630, 870, 2325
Bsu15I	ATCGAT	1 cut(s)	1395
Bsu36I	CCTNAGG	1 cut(s)	1836
BsuRI	GGCC	6 cut(s)	293, 1228, 1452, 1714, 2114, 2133
BsuTUI	ATCGAT	1 cut(s)	1395
BtgZI	GCGATG	1 cut(s)	2084
BtrI	CACGTC	1 cut(s)	1368
BtsCI	GGATG	7 cut(s)	811, 1099, 1501, 1580, 1722, 1773, 2050
BtsI	GCAGTG	1 cut(s)	898
BtsIMutI	CAGTG	4 cut(s)	215, 867, 898, 2100
BveI	ACCTGC	1 cut(s)	662
Cac8I	GCNNGC	5 cut(s)	321, 1450, 1521, 1947, 2112
CaiI	CAGNNNCTG	1 cut(s)	2315
CciI	TCATGA	1 cut(s)	1959
CfoI	GCGC	3 cut(s)	1521, 1523, 1942
Cfr10I	RCCGGY	2 cut(s)	587, 2110
Cfr13I	GGNCC	6 cut(s)	291, 709, 1184, 1226, 2113, 2245
Cfr9I	CCCGGG	2 cut(s)	727, 2115
ClaI	ATCGAT	1 cut(s)	1395
CseI	GACGC	1 cut(s)	1155
Csp6I	GTAC	2 cut(s)	1552, 2002
CviQI	GTAC	2 cut(s)	1552, 2002
DdeI	CTNAG	6 cut(s)	510, 1836, 2135, 2161, 2294, 2421
DpnI	GATC	7 cut(s)	368, 1068, 1470, 1482, 1964, 2017, 2038
DpnII	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
DraIII	CACNNNGTG	1 cut(s)	904
DrdI	GACNNNNNNGTC	2 cut(s)	86, 2327
DseDI	GACNNNNNNGTC	2 cut(s)	86, 2327
EaeI	YGGCCR	1 cut(s)	1712
Eam1104I	CTCTTC	1 cut(s)	827
EarI	CTCTTC	1 cut(s)	827
Ecl136II	GAGCTC	1 cut(s)	1805
Eco130I	CCWWGG	6 cut(s)	484, 1187, 1196, 1815, 1861, 2367
Eco147I	AGGCCT	1 cut(s)	1452
Eco24I	GRGCYC	1 cut(s)	1807
Eco47I	GGWCC	3 cut(s)	709, 1184, 2245
Eco53kI	GAGCTC	1 cut(s)	1805
Eco57I	CTGAAG	2 cut(s)	851, 2337
Eco72I	CACGTG	1 cut(s)	1474
Eco81I	CCTNAGG	1 cut(s)	1836
Eco88I	CYCGRG	3 cut(s)	727, 2115, 2381
Eco91I	GGTNACC	1 cut(s)	1865
EcoICRI	GAGCTC	1 cut(s)	1805
EcoO109I	RGGNCCY	3 cut(s)	709, 1226, 2245
EcoO65I	GGTNACC	1 cut(s)	1865
EcoRI	GAATTC	2 cut(s)	479, 2376
EcoRII	CCWGG	5 cut(s)	441, 1442, 2051, 2281, 2341
EcoT14I	CCWWGG	6 cut(s)	484, 1187, 1196, 1815, 1861, 2367
EcoT38I	GRGCYC	1 cut(s)	1807
ErhI	CCWWGG	6 cut(s)	484, 1187, 1196, 1815, 1861, 2367
Esp3I	CGTCTC	1 cut(s)	1358
FalI	AAGNNNNNCTT	2 cut(s)	1180, 1212
FaqI	GGGAC	7 cut(s)	539, 722, 897, 1253, 1805, 2133, 2258
FblI	GTMKAC	2 cut(s)	351, 1692
Fnu4HI	GCNGC	8 cut(s)	39, 324, 733, 1331, 1407, 1440, 1943, 2168
FokI	GGATG	7 cut(s)	818, 1086, 1508, 1587, 1709, 1780, 2057
FriOI	GRGCYC	1 cut(s)	1807
Fsp4HI	GCNGC	8 cut(s)	39, 324, 733, 1331, 1407, 1440, 1943, 2168
FspBI	CTAG	2 cut(s)	116, 177
GlaI	GCGC	3 cut(s)	1520, 1522, 1941
GluI	GCNGC	8 cut(s)	39, 324, 733, 1331, 1407, 1440, 1943, 2168
GsaI	CCCAGC	1 cut(s)	622
GsuI	CTGGAG	1 cut(s)	1323
HaeIII	GGCC	6 cut(s)	293, 1228, 1452, 1714, 2114, 2133
HgaI	GACGC	1 cut(s)	1155
HhaI	GCGC	3 cut(s)	1521, 1523, 1942
Hin1I	GRCGYC	1 cut(s)	1242
Hin6I	GCGC	3 cut(s)	1519, 1521, 1940
HinP1I	GCGC	3 cut(s)	1519, 1521, 1940
HindIII	AAGCTT	1 cut(s)	282
HinfI	GANTC	6 cut(s)	80, 132, 218, 1416, 1424, 2057
HphI	GGTGA	7 cut(s)	227, 559, 832, 971, 1450, 2230, 2285
Hpy166II	GTNNAC	9 cut(s)	239, 352, 439, 898, 972, 1000, 1371, 1693, 2221
Hpy8I	GTNNAC	9 cut(s)	239, 352, 439, 898, 972, 1000, 1371, 1693, 2221
Hpy99I	CGWCG	1 cut(s)	1171
HpyAV	CCTTC	8 cut(s)	300, 760, 770, 1016, 1104, 1217, 1279, 2415
HpyCH4III	ACNGT	9 cut(s)	49, 61, 236, 719, 1375, 1644, 1690, 1741, 1934
HpyCH4IV	ACGT	5 cut(s)	400, 435, 1242, 1367, 1473
HpyF10VI	GCNNNNNNNGC	8 cut(s)	290, 1172, 1445, 1711, 1811, 1946, 1951, 2075
HpyF3I	CTNAG	6 cut(s)	510, 1836, 2135, 2161, 2294, 2421
HpySE526I	ACGT	5 cut(s)	400, 435, 1242, 1367, 1473
Hsp92I	GRCGYC	1 cut(s)	1242
HspAI	GCGC	3 cut(s)	1519, 1521, 1940
KflI	GGGWCCC	1 cut(s)	709
Kpn2I	TCCGGA	3 cut(s)	128, 1903, 2359
KroI	GCCGGC	1 cut(s)	2110
KroNI	GCCGGC	1 cut(s)	2112
Kzo9I	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
LmnI	GCTCC	6 cut(s)	1163, 1192, 1394, 1403, 2048, 2075
Lsp1109I	GCAGC	6 cut(s)	25, 335, 1393, 1426, 1954, 2154
LweI	GCATC	7 cut(s)	605, 985, 1426, 1486, 1633, 1692, 1731
MaeI	CTAG	2 cut(s)	116, 177
MaeII	ACGT	5 cut(s)	400, 435, 1242, 1367, 1473
MalI	GATC	7 cut(s)	368, 1068, 1470, 1482, 1964, 2017, 2038
MboI	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
MboII	GAAGA	9 cut(s)	287, 635, 775, 844, 875, 997, 1445, 1888, 2330
MfeI	CAATTG	1 cut(s)	144
MhlI	GDGCHC	2 cut(s)	241, 1807
MlsI	TGGCCA	1 cut(s)	1714
MluNI	TGGCCA	1 cut(s)	1714
MlyI	GAGTC	3 cut(s)	74, 126, 1418
MmeI	TCCRAC	3 cut(s)	148, 1008, 1200
Mox20I	TGGCCA	1 cut(s)	1714
MroI	TCCGGA	3 cut(s)	128, 1903, 2359
MroNI	GCCGGC	1 cut(s)	2110
MroXI	GAANNNNTTC	2 cut(s)	222, 2355
MscI	TGGCCA	1 cut(s)	1714
MseI	TTAA	3 cut(s)	363, 432, 759
MslI	CAYNNNNRTG	3 cut(s)	1134, 1325, 1700
Msp20I	TGGCCA	1 cut(s)	1714
MspCI	CTTAAG	2 cut(s)	362, 758
MunI	CAATTG	1 cut(s)	144
Mva1269I	GAATGC	4 cut(s)	1409, 1488, 1579, 2210
MvaI	CCWGG	5 cut(s)	443, 1444, 2053, 2283, 2343
MvnI	CGCG	2 cut(s)	1521, 1872
MwoI	GCNNNNNNNGC	8 cut(s)	290, 1172, 1445, 1711, 1811, 1946, 1951, 2075
NaeI	GCCGGC	1 cut(s)	2112
NciI	CCSGG	7 cut(s)	728, 729, 1462, 1727, 1887, 2116, 2117
NdeII	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
NgoMIV	GCCGGC	1 cut(s)	2110
NlaIV	GGNNCC	9 cut(s)	127, 292, 592, 710, 711, 1194, 2050, 2077, 2246
NmuCI	GTSAC	5 cut(s)	1456, 1644, 2258, 2273, 2329
NspI	RCATGY	1 cut(s)	319
NspV	TTCGAA	1 cut(s)	404
PagI	TCATGA	1 cut(s)	1959
PauI	GCGCGC	1 cut(s)	1519
PceI	AGGCCT	1 cut(s)	1452
PcsI	WCGNNNNNNNCGW	1 cut(s)	2066
PctI	GAATGC	4 cut(s)	1409, 1488, 1579, 2210
PdiI	GCCGGC	1 cut(s)	2112
PdmI	GAANNNNTTC	2 cut(s)	222, 2355
PfeI	GAWTC	3 cut(s)	218, 1416, 2057
PflMI	CCANNNNNTGG	1 cut(s)	1919
PfoI	TCCNGGA	1 cut(s)	1725
PkrI	GCNGC	8 cut(s)	40, 325, 734, 1332, 1408, 1441, 1944, 2169
PleI	GAGTC	3 cut(s)	74, 126, 1418
PmaCI	CACGTG	1 cut(s)	1474
PmlI	CACGTG	1 cut(s)	1474
PpsI	GAGTC	3 cut(s)	74, 126, 1418
Ppu21I	YACGTR	1 cut(s)	1474
PpuMI	RGGWCCY	2 cut(s)	709, 2245
Psp124BI	GAGCTC	1 cut(s)	1807
Psp5II	RGGWCCY	2 cut(s)	709, 2245
Psp6I	CCWGG	5 cut(s)	441, 1442, 2051, 2281, 2341
PspCI	CACGTG	1 cut(s)	1474
PspEI	GGTNACC	1 cut(s)	1865
PspFI	CCCAGC	1 cut(s)	618
PspGI	CCWGG	5 cut(s)	441, 1442, 2051, 2281, 2341
PspN4I	GGNNCC	9 cut(s)	127, 292, 592, 710, 711, 1194, 2050, 2077, 2246
PspPI	GGNCC	6 cut(s)	291, 709, 1184, 1226, 2113, 2245
PspPPI	RGGWCCY	2 cut(s)	709, 2245
PstNI	CAGNNNCTG	1 cut(s)	2315
PteI	GCGCGC	1 cut(s)	1519
RsaI	GTAC	2 cut(s)	1553, 2003
RsaNI	GTAC	2 cut(s)	1552, 2002
RseI	CAYNNNNRTG	3 cut(s)	1134, 1325, 1700
SacI	GAGCTC	1 cut(s)	1807
SaqAI	TTAA	3 cut(s)	363, 432, 759
SatI	GCNGC	8 cut(s)	39, 324, 733, 1331, 1407, 1440, 1943, 2168
Sau3AI	GATC	7 cut(s)	366, 1066, 1468, 1480, 1962, 2015, 2036
Sau96I	GGNCC	6 cut(s)	291, 709, 1184, 1226, 2113, 2245
ScaI	AGTACT	1 cut(s)	1553
SchI	GAGTC	3 cut(s)	74, 126, 1418
SduI	GDGCHC	2 cut(s)	241, 1807
SfaNI	GCATC	7 cut(s)	605, 985, 1426, 1486, 1633, 1692, 1731
SfcI	CTRYAG	1 cut(s)	45
SfuI	TTCGAA	1 cut(s)	404
SinI	GGWCC	3 cut(s)	709, 1184, 2245
SmaI	CCCGGG	2 cut(s)	729, 2117
SmiMI	CAYNNNNRTG	3 cut(s)	1134, 1325, 1700
SmlI	CTYRAG	3 cut(s)	362, 758, 1353
SmoI	CTYRAG	3 cut(s)	362, 758, 1353
SseBI	AGGCCT	1 cut(s)	1452
SsiI	CCGC	5 cut(s)	732, 1182, 1331, 1870, 1954
SspI	AATATT	1 cut(s)	1752
SspMI	CTAG	2 cut(s)	116, 177
SstI	GAGCTC	1 cut(s)	1807
StuI	AGGCCT	1 cut(s)	1452
StyI	CCWWGG	6 cut(s)	484, 1187, 1196, 1815, 1861, 2367
TaaI	ACNGT	9 cut(s)	49, 61, 236, 719, 1375, 1644, 1690, 1741, 1934
TaiI	ACGT	5 cut(s)	403, 438, 1245, 1370, 1476
TatI	WGTACW	2 cut(s)	1551, 2001
TauI	GCSGC	2 cut(s)	735, 1333
TfiI	GAWTC	3 cut(s)	218, 1416, 2057
Tru1I	TTAA	3 cut(s)	363, 432, 759
Tru9I	TTAA	3 cut(s)	363, 432, 759
TscAI	CASTG	4 cut(s)	215, 874, 905, 2107
TseFI	GTSAC	5 cut(s)	1456, 1644, 2258, 2273, 2329
TseI	GCWGC	6 cut(s)	38, 323, 1406, 1439, 1942, 2167
Tsp45I	GTSAC	5 cut(s)	1456, 1644, 2258, 2273, 2329
TspGWI	ACGGA	2 cut(s)	2337, 2348
TspMI	CCCGGG	2 cut(s)	727, 2115
TspRI	CASTG	4 cut(s)	215, 874, 905, 2107
Van91I	CCANNNNNTGG	1 cut(s)	1919
Vha464I	CTTAAG	2 cut(s)	362, 758
VneI	GTGCAC	1 cut(s)	237
VpaK11BI	GGWCC	3 cut(s)	709, 1184, 2245
XapI	RAATTY	7 cut(s)	408, 479, 563, 576, 2124, 2351, 2376
XceI	RCATGY	1 cut(s)	319
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1194, 2099
XmaI	CCCGGG	2 cut(s)	727, 2115
XmiI	GTMKAC	2 cut(s)	351, 1692
XmnI	GAANNNNTTC	2 cut(s)	222, 2355
XspI	CTAG	2 cut(s)	116, 177
ZraI	GACGTC	1 cut(s)	1243
ZrmI	AGTACT	1 cut(s)	1553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.