RLG00000015940

Phospholipase D alpha 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010738
Physical Location & Seq
Reverse (-)
2642485 .. 2645441
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015940

Sequence Viewer

Length: 2430 bp
ATGGCGCAAGCTGTGCTGCTGCATGGAGTACTTCATGCAACGATTTTCGAAGTCGATAAGTTGAAGGCTGGAAATTGCTGCACCTTCTTATGCAAGCTGATTGAAGAGTCAGTTGGGTTGGGGAAAGGTTGCAGACCCTATGCAACCATTGATTTGGAGAATGAAAGAGTTGGGAGAACTAGACTGCTGGAAGAAACAACCACAAATCCACAGTGGAATGAATCCTTTCACATTTACTGTGCTCATATGGCTTCTAATGTCGTTTTCTCCATCAAAGAATACAATCCAATCGGGGCCAATGTCATCGGAAGAGCTTATTTGCCTGCTGCTGAACTCCTCAATGGAGAAGAAGTAGACAGATGGCTTAAGATCAGAATGGATCATCACAGTAGACCTTTACATGGAGGTTCCAAAATACATGTGAAACTGAGGTTTCATCCTGTAACTAAAAACTGTAATTGGTCTCATGGAATCAAAAGTCCTAAATATCCAGGAGTACCCTACACATTTTTCCCACAAAGAAATGGCTGCAGGGTTACCCTTTACCAAGATGCTCATGTTCCAGACAATTTCATCCCCAACATCCCTTTAGCTGGAGGCAAGTGTTACGAACCTCATCGGTGTTGGGAAGATATTTTTCATGCAATCTATAATGCAAAGCACTTGATATACATTGCTGGCTGGTCTGTATATACTAAAATCACGTTAGTAAGGGACACGATGAGGCCAAAGATGGTAGACGGAGACTTGACTCTGGGAGAGCTTCTTAAGAAGAAAGCAAAGGAAGGAGTGAAGGTCTTAATGCTTGTATGGGATGACAGAACTTCAGTGAAGGTACTAAAGAAAGATGGACTGATGGCTACTCATGATGAAGATACCGAAAGTTACTTCCATGACAGCGGAGTTCACTGCGTGTTGTGTCCCAGAAACCCTGACAACGGGCAAAGCATTGTTCAAGATATAGAGGTTGGAACAATGTTCACTCATCATCAAAAGATTGTGGTAGTGGACAGTGAATTGCCGAATGGAGCATTAGAAAAGAGGAGGATTGTGAGCTTCATTGGCGGCATTGATCTTTGTGATGGGAGATATGACACGCAGTTTCATCCCCTTTTCAGGACTTTAGGCACAATCCACCATGATGATCTCCATCAACCCAATTTTAATGGTGCCTCAATCGCAAAAGGTGGACCAAGGGAGCCTTGGCATGACATACATTGTAGGCTAGAAGGGCCTATTGCTTGGGATGTTTTATTCAACTTTGAGCAGAGGTGGAGAAAGCAAGGTGTGAAAGACTTACTTTTTCAACTCAGAGATTTTGATGAGACCTTTATACCACCATCTCCTGTTATGTATCCTGAAGACCATGACACATGGAATGTTCAGTTGTTCCGATCCATTGATGGAGGAGCTGCATTCGGATTTCCCAACTCTCCTGAAGATGCTACCAGGGCTGGTCTAGTCAGTGGCAGAGATCATGTGATTGACAGAAGCATTCAGGATGCATATATCAATGCAATTCGTCGAGCAAAGAGTTTTATCTACATCGAAAATCAGTACTTTCTTGGAAGCTCTTTCGGCTGGCAGTCAGATCATAAAGACCTCAAAGTTGAGGAGGTTGGCGCTTTGCATCTTATTCCAAAAGAACTGTCACTAAAGATTGTTAGTAAGATTGAAGCTGGAGAAAGATTTACTGTCTACATTGTCATGCCTATGTGGCCAGAGGGTGTTCCAGAAAGCCAATCTGTTCAGGCAATATTGCATTGGCAGAAGTTGACAATGGAGATGATGTATAGGGACATAACACAAGCTCTAAAGACCAAGGGGCTTGATGCAAACCCTAAGGAGTATTTGACATTCTTTTGCCTTGGAAATCGTGAGAAGAAGCGAAGCGGAGAATATAAACCTCATGAGAAACCACAGCATGATACAGATTACAGTAGAGCTCAACAATCTAGGCGGTTCATGATCTACGTACATGCCAAGTGTATGATAGTTGATGATGAATACATAATCATTGGATCCGCCAACATAAATCAGAGATCAATGGACGGCGCCAGGGATACCGAGATTGCCATGGGAGCTTACCAACCATATCACCTATCTACCAATATACCTGCCCGGGGCCAAATCCATGGCCTCAGAATGGCATTGTGGTATGAACATTTGGGGTTGCTCAATGACACCTTTCTTGAGCCGGAGAGTGTGGAATGTGTGAGAAAGGTGAACCAGATTGCCGAAAAGCATTGGGAACTCTATTTAAGTGAGACCTTAGAGGGTGACTTGCCAGGACATTTGCTACCCTATCCCATAAAAATTACTGAAAATGGAGAGGTCACTGAGTTACCTGGGGTTGAATTTTTTCCAGACACCAAGGGTAAAGTTCTTGGAAGCAGATCTGACTTAATTCCTTCAATCATCACTACTTAA

Protein Analysis

810

Amino Acids

92.25

Weight (kDa)

6.35

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 41 - 123 1.1e-10 C2 domain
PLDc PF00614 326 - 363 1.2e-06 Phospholipase D Active site motif
PLDc_2 PF13091 654 - 691 9e-07 PLD-like domain
PLDc PF00614 657 - 682 6.9e-07 Phospholipase D Active site motif
PLD_C PF12357 728 - 798 3.3e-30 Phospholipase D C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010738)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25370 AT5G25370 AT5G25370 AT5G25370
fragaria_vesca FvH4_1g03170
malus_domestica MD02G1030700.v1.1 MD15G1172300.v1.1
prunus_persica Prupe.7G243800_v2.0.a1
pyrus_communis pycom02g02560 pycom15g15370
rosa_chinensis RchiOBHm_Chr2g0088301
rosa_laevigata RLG00000015940
rosa_multiflora Rmu_sc0004235.1_g000036
rosa_roxburghii Rroxscaffold_2G00152760
rosa_rugosa Rorug01G0482700
rosa_samantha Rh2AG037800 Rh2BG037000 Rh2CG038100 Rh2DG037500
rosa_wichuraiana Rw2G002980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2125
AccB1I GGYRCC 2 cut(s) 1169, 2054
AccI GTMKAC 4 cut(s) 354, 391, 738, 1698
AciI CCGC 5 cut(s) 900, 1065, 1893, 1960, 2025
AclWI GGATC 4 cut(s) 387, 1389, 2016, 2029
AcoI YGGCCR 1 cut(s) 1718
AcsI RAATTY 1 cut(s) 2357
AcuI CTGAAG 3 cut(s) 810, 1380, 1458
AcyI GRCGYC 1 cut(s) 2055
AdeI CACNNNGTG 1 cut(s) 913
AfaI GTAC 5 cut(s) 30, 498, 837, 1559, 1977
AfiI CCNNNNNNNGG 6 cut(s) 401, 593, 938, 1116, 2123, 2146
AflII CTTAAG 2 cut(s) 365, 767
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 8 cut(s) 64, 104, 956, 1258, 1307, 1676, 2357, 2415
AjnI CCWGG 5 cut(s) 490, 1448, 2057, 2287, 2347
AjuI GAANNNNNNNTTGG 2 cut(s) 96, 128
Alw21I GWGCWC 2 cut(s) 244, 1948
Alw26I GTCTC 4 cut(s) 468, 738, 1319, 2261
AlwI GGATC 4 cut(s) 387, 1389, 2016, 2029
Ama87I CYCGRG 1 cut(s) 2121
AoxI GGCC 6 cut(s) 294, 725, 1232, 1718, 2125, 2137
ApeKI GCWGC 6 cut(s) 16, 19, 78, 326, 528, 1412
ApoI RAATTY 1 cut(s) 2357
ArsI GACNNNNNNTTYG 2 cut(s) 1634, 1666
Asp700I GAANNNNTTC 2 cut(s) 225, 2361
AspLEI GCGC 3 cut(s) 7, 1625, 2057
AspS9I GGNCC 4 cut(s) 294, 1190, 1232, 2125
AsuC2I CCSGG 2 cut(s) 2122, 2123
AsuHPI GGTGA 3 cut(s) 2090, 2236, 2291
AsuII TTCGAA 1 cut(s) 48
AvaI CYCGRG 1 cut(s) 2121
AvaII GGWCC 1 cut(s) 1190
AxyI CCTNAGG 1 cut(s) 1842
BalI TGGCCA 1 cut(s) 1720
BamHI GGATCC 1 cut(s) 2021
BanI GGYRCC 2 cut(s) 1169, 2054
BanII GRGCYC 1 cut(s) 1948
BbsI GAAGAC 1 cut(s) 1368
Bbv12I GWGCWC 2 cut(s) 244, 1948
BbvI GCAGC 6 cut(s) 3, 6, 65, 313, 515, 1399
BccI CCATC 9 cut(s) 278, 354, 727, 842, 850, 1076, 1158, 1348, 1397
BceAI ACGGC 1 cut(s) 2068
BciT130I CCWGG 5 cut(s) 492, 1450, 2059, 2289, 2349
BciVI GTATCC 2 cut(s) 1365, 2056
BcnI CCSGG 2 cut(s) 2122, 2123
BcoDI GTCTC 4 cut(s) 468, 738, 1319, 2261
BfaI CTAG 4 cut(s) 180, 1226, 1460, 1956
BfmI CTRYAG 1 cut(s) 529
BfoI RGCGCY 2 cut(s) 1626, 2058
BfrI CTTAAG 2 cut(s) 365, 767
BfuAI ACCTGC 1 cut(s) 2125
BfuI GTATCC 2 cut(s) 1365, 2056
BglI GCCNNNNNGGC 1 cut(s) 1717
BglII AGATCT 1 cut(s) 2396
BisI GCNGC 7 cut(s) 17, 20, 79, 327, 529, 1066, 1413
BlsI GCNGC 7 cut(s) 18, 21, 80, 328, 530, 1067, 1414
BmcAI AGTACT 2 cut(s) 30, 1559
Bme1390I CCNGG 7 cut(s) 492, 1450, 2059, 2122, 2123, 2289, 2349
Bme18I GGWCC 1 cut(s) 1190
BmeT110I CYCGRG 1 cut(s) 2121
BmgT120I GGNCC 4 cut(s) 294, 1190, 1232, 2125
BmiI GGNNCC 7 cut(s) 295, 409, 1171, 1200, 2023, 2056, 2126
BmrFI CCNGG 7 cut(s) 492, 1450, 2059, 2122, 2123, 2289, 2349
BmsI GCATC 5 cut(s) 541, 1432, 1492, 1639, 1822
BpiI GAAGAC 1 cut(s) 1368
BpmI CTGGAG 2 cut(s) 615, 1701
Bpu14I TTCGAA 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 2213
BpuMI CCSGG 2 cut(s) 2122, 2123
BsaAI YACGTR 1 cut(s) 1975
BsaBI GATNNNNATC 1 cut(s) 1149
BsaHI GRCGYC 1 cut(s) 2055
BsaI GGTCTC 3 cut(s) 468, 1319, 2261
BsaXI ACNNNNNCTCC 6 cut(s) 336, 366, 780, 810, 1775, 1805
Bsc4I CCNNNNNNNGG 6 cut(s) 401, 593, 938, 1116, 2123, 2146
Bse21I CCTNAGG 1 cut(s) 1842
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 1149
BseBI CCWGG 5 cut(s) 492, 1450, 2059, 2289, 2349
BseGI GGATG 7 cut(s) 436, 573, 582, 820, 1105, 1252, 1507
BseJI GATNNNNATC 1 cut(s) 1149
BseLI CCNNNNNNNGG 6 cut(s) 401, 593, 938, 1116, 2123, 2146
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 4 cut(s) 419, 1324, 2155, 2331
BseRI GAGGAG 4 cut(s) 326, 1057, 1422, 1628
BseXI GCAGC 6 cut(s) 3, 6, 65, 313, 515, 1399
BsgI GTGCAG 1 cut(s) 64
BshFI GGCC 6 cut(s) 296, 727, 1234, 1720, 2127, 2139
BshNI GGYRCC 2 cut(s) 1169, 2054
BsiHKAI GWGCWC 2 cut(s) 244, 1948
BsiHKCI CYCGRG 1 cut(s) 2121
BsiSI CCGG 2 cut(s) 2122, 2198
BslFI GGGAC 3 cut(s) 728, 906, 1811
BslI CCNNNNNNNGG 6 cut(s) 401, 593, 938, 1116, 2123, 2146
BsmAI GTCTC 4 cut(s) 468, 738, 1319, 2261
BsmFI GGGAC 3 cut(s) 728, 906, 1811
BsmI GAATGC 2 cut(s) 1415, 1494
BsnI GGCC 6 cut(s) 296, 727, 1234, 1720, 2127, 2139
Bso31I GGTCTC 3 cut(s) 468, 1319, 2261
BsoBI CYCGRG 1 cut(s) 2121
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 2 cut(s) 244, 1948
Bsp19I CCATGG 2 cut(s) 2076, 2134
BspACI CCGC 5 cut(s) 900, 1065, 1893, 1960, 2025
BspANI GGCC 6 cut(s) 296, 727, 1234, 1720, 2127, 2139
BspCNI CTCAG 4 cut(s) 420, 1323, 2154, 2332
BspHI TCATGA 3 cut(s) 865, 1909, 1965
BspLI GGNNCC 7 cut(s) 295, 409, 1171, 1200, 2023, 2056, 2126
BspMAI CTGCAG 1 cut(s) 533
BspMI ACCTGC 1 cut(s) 2125
BspPI GGATC 4 cut(s) 387, 1389, 2016, 2029
BspQI GCTCTTC 1 cut(s) 304
BspT104I TTCGAA 1 cut(s) 48
BspT107I GGYRCC 2 cut(s) 1169, 2054
BspTI CTTAAG 2 cut(s) 365, 767
BspTNI GGTCTC 3 cut(s) 468, 1319, 2261
BsrDI GCAATG 1 cut(s) 672
BssNI GRCGYC 1 cut(s) 2055
BssT1I CCWWGG 7 cut(s) 1193, 1202, 1821, 1867, 2076, 2134, 2373
Bst2UI CCWGG 5 cut(s) 492, 1450, 2059, 2289, 2349
Bst4CI ACNGT 8 cut(s) 213, 239, 389, 455, 1013, 1650, 1696, 1940
Bst6I CTCTTC 2 cut(s) 99, 304
BstACI GRCGYC 1 cut(s) 2055
BstAFI CTTAAG 2 cut(s) 365, 767
BstAPI GCANNNNNTGC 1 cut(s) 13
BstBAI YACGTR 1 cut(s) 1975
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 5 cut(s) 9, 95, 324, 679, 1583
BstDEI CTNAG 6 cut(s) 428, 1310, 1842, 2141, 2272, 2340
BstDSI CCRYGG 2 cut(s) 2076, 2134
BstEII GGTNACC 1 cut(s) 535
BstF5I GGATG 7 cut(s) 436, 573, 582, 820, 1105, 1252, 1507
BstH2I RGCGCY 2 cut(s) 1626, 2058
BstHHI GCGC 3 cut(s) 7, 1625, 2057
BstMAI GTCTC 4 cut(s) 468, 738, 1319, 2261
BstMWI GCNNNNNNNGC 9 cut(s) 13, 248, 1062, 1178, 1231, 1451, 1578, 1717, 2081
BstNI CCWGG 5 cut(s) 492, 1450, 2059, 2289, 2349
BstNSI RCATGY 2 cut(s) 422, 1982
BstPI GGTNACC 1 cut(s) 535
BstSCI CCNGG 7 cut(s) 490, 1448, 2057, 2120, 2121, 2287, 2347
BstSFI CTRYAG 1 cut(s) 529
BstSNI TACGTA 1 cut(s) 1975
BstV1I GCAGC 6 cut(s) 3, 6, 65, 313, 515, 1399
BstV2I GAAGAC 1 cut(s) 1368
BstX2I RGATCY 2 cut(s) 2021, 2396
BstXI CCANNNNNNTGG 2 cut(s) 154, 2135
BstYI RGATCY 2 cut(s) 2021, 2396
Bsu36I CCTNAGG 1 cut(s) 1842
BsuI GTATCC 2 cut(s) 1365, 2056
BsuRI GGCC 6 cut(s) 296, 727, 1234, 1720, 2127, 2139
BtgI CCRYGG 2 cut(s) 2076, 2134
BtsCI GGATG 7 cut(s) 436, 573, 582, 820, 1105, 1252, 1507
BtsI GCAGTG 1 cut(s) 907
BtsIMutI CAGTG 6 cut(s) 218, 834, 907, 1018, 1471, 2337
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 5 cut(s) 9, 95, 324, 679, 1583
CciI TCATGA 3 cut(s) 865, 1909, 1965
CfoI GCGC 3 cut(s) 7, 1625, 2057
Cfr13I GGNCC 4 cut(s) 294, 1190, 1232, 2125
Cfr9I CCCGGG 1 cut(s) 2121
Csp6I GTAC 5 cut(s) 29, 497, 836, 1558, 1976
CviQI GTAC 5 cut(s) 29, 497, 836, 1558, 1976
DdeI CTNAG 6 cut(s) 428, 1310, 1842, 2141, 2272, 2340
DinI GGCGCC 1 cut(s) 2056
DraIII CACNNNGTG 1 cut(s) 913
EaeI YGGCCR 1 cut(s) 1718
Eam1104I CTCTTC 2 cut(s) 99, 304
EarI CTCTTC 2 cut(s) 99, 304
EciI GGCGGA 1 cut(s) 2014
Ecl136II GAGCTC 1 cut(s) 1946
Eco105I TACGTA 1 cut(s) 1975
Eco130I CCWWGG 7 cut(s) 1193, 1202, 1821, 1867, 2076, 2134, 2373
Eco24I GRGCYC 1 cut(s) 1948
Eco31I GGTCTC 3 cut(s) 468, 1319, 2261
Eco47I GGWCC 1 cut(s) 1190
Eco53kI GAGCTC 1 cut(s) 1946
Eco57I CTGAAG 3 cut(s) 810, 1380, 1458
Eco81I CCTNAGG 1 cut(s) 1842
Eco88I CYCGRG 1 cut(s) 2121
Eco91I GGTNACC 1 cut(s) 535
EcoICRI GAGCTC 1 cut(s) 1946
EcoO109I RGGNCCY 1 cut(s) 1232
EcoO65I GGTNACC 1 cut(s) 535
EcoRII CCWGG 5 cut(s) 490, 1448, 2057, 2287, 2347
EcoT14I CCWWGG 7 cut(s) 1193, 1202, 1821, 1867, 2076, 2134, 2373
EcoT22I ATGCAT 1 cut(s) 1507
EcoT38I GRGCYC 1 cut(s) 1948
EgeI GGCGCC 1 cut(s) 2056
EheI GGCGCC 1 cut(s) 2056
ErhI CCWWGG 7 cut(s) 1193, 1202, 1821, 1867, 2076, 2134, 2373
FalI AAGNNNNNCTT 4 cut(s) 1186, 1218, 1284, 1316
FaqI GGGAC 3 cut(s) 728, 906, 1811
FauNDI CATATG 1 cut(s) 246
FblI GTMKAC 4 cut(s) 354, 391, 738, 1698
Fnu4HI GCNGC 7 cut(s) 17, 20, 79, 327, 529, 1066, 1413
FokI GGATG 7 cut(s) 423, 560, 569, 827, 1092, 1259, 1514
FriOI GRGCYC 1 cut(s) 1948
Fsp4HI GCNGC 7 cut(s) 17, 20, 79, 327, 529, 1066, 1413
FspBI CTAG 4 cut(s) 180, 1226, 1460, 1956
GlaI GCGC 3 cut(s) 6, 1624, 2056
GluI GCNGC 7 cut(s) 17, 20, 79, 327, 529, 1066, 1413
GsuI CTGGAG 2 cut(s) 615, 1701
HaeII RGCGCY 2 cut(s) 1626, 2058
HaeIII GGCC 6 cut(s) 296, 727, 1234, 1720, 2127, 2139
HapII CCGG 2 cut(s) 2122, 2198
HhaI GCGC 3 cut(s) 7, 1625, 2057
Hin1I GRCGYC 1 cut(s) 2055
Hin6I GCGC 3 cut(s) 5, 1623, 2055
HinP1I GCGC 3 cut(s) 5, 1623, 2055
HincII GTYRAC 1 cut(s) 1776
HindII GTYRAC 1 cut(s) 1776
HinfI GANTC 4 cut(s) 107, 221, 471, 751
HpaII CCGG 2 cut(s) 2122, 2198
HphI GGTGA 3 cut(s) 2090, 2236, 2291
Hpy188I TCNGA 9 cut(s) 308, 374, 1313, 1394, 1421, 1591, 2040, 2144, 2401
Hpy99I CGWCG 1 cut(s) 1527
HpyAV CCTTC 7 cut(s) 58, 94, 779, 787, 826, 1223, 2421
HpyCH4III ACNGT 8 cut(s) 213, 239, 389, 455, 1013, 1650, 1696, 1940
HpyCH4IV ACGT 2 cut(s) 704, 1974
HpyF10VI GCNNNNNNNGC 9 cut(s) 13, 248, 1062, 1178, 1231, 1451, 1578, 1717, 2081
HpyF3I CTNAG 6 cut(s) 428, 1310, 1842, 2141, 2272, 2340
HpySE526I ACGT 2 cut(s) 704, 1974
Hsp92I GRCGYC 1 cut(s) 2055
HspAI GCGC 3 cut(s) 5, 1623, 2055
KasI GGCGCC 1 cut(s) 2054
LguI GCTCTTC 1 cut(s) 304
LmnI GCTCC 4 cut(s) 1028, 1198, 1409, 2081
Lsp1109I GCAGC 6 cut(s) 3, 6, 65, 313, 515, 1399
LweI GCATC 5 cut(s) 541, 1432, 1492, 1639, 1822
MaeI CTAG 4 cut(s) 180, 1226, 1460, 1956
MaeII ACGT 2 cut(s) 704, 1974
MaeIII GTNAC 8 cut(s) 442, 535, 605, 884, 1650, 2279, 2335, 2343
MflI RGATCY 2 cut(s) 2021, 2396
MhlI GDGCHC 2 cut(s) 244, 1948
MlsI TGGCCA 1 cut(s) 1720
MluCI AATT 9 cut(s) 73, 457, 568, 1016, 1159, 1518, 2316, 2357, 2406
MluNI TGGCCA 1 cut(s) 1720
Mly113I GGCGCC 1 cut(s) 2055
MlyI GAGTC 2 cut(s) 116, 745
MmeI TCCRAC 1 cut(s) 949
Mox20I TGGCCA 1 cut(s) 1720
Mph1103I ATGCAT 1 cut(s) 1507
MroXI GAANNNNTTC 2 cut(s) 225, 2361
MscI TGGCCA 1 cut(s) 1720
MseI TTAA 7 cut(s) 366, 768, 800, 1164, 2261, 2405, 2428
MslI CAYNNNNRTG 3 cut(s) 1140, 1706, 1712
Msp20I TGGCCA 1 cut(s) 1720
MspA1I CMGCKG 1 cut(s) 900
MspCI CTTAAG 2 cut(s) 365, 767
MspI CCGG 2 cut(s) 2122, 2198
MspR9I CCNGG 7 cut(s) 492, 1450, 2059, 2122, 2123, 2289, 2349
Mva1269I GAATGC 2 cut(s) 1415, 1494
MvaI CCWGG 5 cut(s) 492, 1450, 2059, 2289, 2349
MwoI GCNNNNNNNGC 9 cut(s) 13, 248, 1062, 1178, 1231, 1451, 1578, 1717, 2081
NarI GGCGCC 1 cut(s) 2055
NciI CCSGG 2 cut(s) 2122, 2123
NcoI CCATGG 2 cut(s) 2076, 2134
NdeI CATATG 1 cut(s) 246
NlaIV GGNNCC 7 cut(s) 295, 409, 1171, 1200, 2023, 2056, 2126
NmuCI GTSAC 3 cut(s) 1650, 2279, 2335
NsiI ATGCAT 1 cut(s) 1507
NspI RCATGY 2 cut(s) 422, 1982
NspV TTCGAA 1 cut(s) 48
PagI TCATGA 3 cut(s) 865, 1909, 1965
PciI ACATGT 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 304
PctI GAATGC 2 cut(s) 1415, 1494
PdmI GAANNNNTTC 2 cut(s) 225, 2361
PfeI GAWTC 2 cut(s) 221, 471
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 7 cut(s) 18, 21, 80, 328, 530, 1067, 1414
PleI GAGTC 2 cut(s) 115, 745
PluTI GGCGCC 1 cut(s) 2058
PpsI GAGTC 2 cut(s) 115, 745
Ppu21I YACGTR 1 cut(s) 1975
PscI ACATGT 1 cut(s) 418
Psp124BI GAGCTC 1 cut(s) 1948
Psp6I CCWGG 5 cut(s) 490, 1448, 2057, 2287, 2347
PspEI GGTNACC 1 cut(s) 535
PspGI CCWGG 5 cut(s) 490, 1448, 2057, 2287, 2347
PspN4I GGNNCC 7 cut(s) 295, 409, 1171, 1200, 2023, 2056, 2126
PspPI GGNCC 4 cut(s) 294, 1190, 1232, 2125
PstI CTGCAG 1 cut(s) 533
PsuI RGATCY 2 cut(s) 2021, 2396
RsaI GTAC 5 cut(s) 30, 498, 837, 1559, 1977
RsaNI GTAC 5 cut(s) 29, 497, 836, 1558, 1976
RseI CAYNNNNRTG 3 cut(s) 1140, 1706, 1712
SacI GAGCTC 1 cut(s) 1948
SapI GCTCTTC 1 cut(s) 304
SaqAI TTAA 7 cut(s) 366, 768, 800, 1164, 2261, 2405, 2428
SatI GCNGC 7 cut(s) 17, 20, 79, 327, 529, 1066, 1413
Sau96I GGNCC 4 cut(s) 294, 1190, 1232, 2125
ScaI AGTACT 2 cut(s) 30, 1559
SchI GAGTC 2 cut(s) 116, 745
ScrFI CCNGG 7 cut(s) 492, 1450, 2059, 2122, 2123, 2289, 2349
SduI GDGCHC 2 cut(s) 244, 1948
SfaNI GCATC 5 cut(s) 541, 1432, 1492, 1639, 1822
SfcI CTRYAG 1 cut(s) 529
SfoI GGCGCC 1 cut(s) 2056
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 1 cut(s) 1190
SmaI CCCGGG 1 cut(s) 2123
SmiMI CAYNNNNRTG 3 cut(s) 1140, 1706, 1712
SmlI CTYRAG 3 cut(s) 365, 767, 2192
SmoI CTYRAG 3 cut(s) 365, 767, 2192
SnaBI TACGTA 1 cut(s) 1975
Sse9I AATT 9 cut(s) 73, 457, 568, 1016, 1159, 1518, 2316, 2357, 2406
SsiI CCGC 5 cut(s) 900, 1065, 1893, 1960, 2025
SspDI GGCGCC 1 cut(s) 2054
SspI AATATT 1 cut(s) 1758
SspMI CTAG 4 cut(s) 180, 1226, 1460, 1956
SstI GAGCTC 1 cut(s) 1948
StyD4I CCNGG 7 cut(s) 490, 1448, 2057, 2120, 2121, 2287, 2347
StyI CCWWGG 7 cut(s) 1193, 1202, 1821, 1867, 2076, 2134, 2373
TaaI ACNGT 8 cut(s) 213, 239, 389, 455, 1013, 1650, 1696, 1940
TaiI ACGT 2 cut(s) 707, 1977
TaqI TCGA 4 cut(s) 48, 54, 1525, 1548
TasI AATT 9 cut(s) 73, 457, 568, 1016, 1159, 1518, 2316, 2357, 2406
TatI WGTACW 2 cut(s) 28, 1557
TauI GCSGC 1 cut(s) 1068
TfiI GAWTC 2 cut(s) 221, 471
Tru1I TTAA 7 cut(s) 366, 768, 800, 1164, 2261, 2405, 2428
Tru9I TTAA 7 cut(s) 366, 768, 800, 1164, 2261, 2405, 2428
TscAI CASTG 6 cut(s) 218, 834, 914, 1018, 1471, 2344
TseFI GTSAC 3 cut(s) 1650, 2279, 2335
TseI GCWGC 6 cut(s) 16, 19, 78, 326, 528, 1412
Tsp45I GTSAC 3 cut(s) 1650, 2279, 2335
TspGWI ACGGA 1 cut(s) 756
TspMI CCCGGG 1 cut(s) 2121
TspRI CASTG 6 cut(s) 218, 834, 914, 1018, 1471, 2344
Vha464I CTTAAG 2 cut(s) 365, 767
VpaK11BI GGWCC 1 cut(s) 1190
XapI RAATTY 1 cut(s) 2357
XceI RCATGY 2 cut(s) 422, 1982
XcmI CCANNNNNNNNNTGG 1 cut(s) 1200
XmaI CCCGGG 1 cut(s) 2121
XmiI GTMKAC 4 cut(s) 354, 391, 738, 1698
XmnI GAANNNNTTC 2 cut(s) 225, 2361
XspI CTAG 4 cut(s) 180, 1226, 1460, 1956
ZrmI AGTACT 2 cut(s) 30, 1559
Zsp2I ATGCAT 1 cut(s) 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.