AT5G27395

Tim44

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013393
Physical Location & Seq
Reverse (-)
9671393 .. 9674267
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27395.2

Sequence Viewer

Length: 942 bp
ATGTCGCTCGTGAGAAGATTTCAAACAGTTCGTTCTTTATTGAAAACAGCTGGATCAAGAGAATCTTCTTCTCTTCCATTTAGGAATGGAAATGAGTTCTCTTTGATTCAAATTGGAGATGATTTCAGATTTGGTTGTTGGAGAAGCTATCATTCTTCTCTTTGTCACGTTCCTGAAGCTCATGGAAAAAGTGCGTATTCACGTTTATATGAAGGCCACAGCGTCAATACACATTTGCTTCGATCTACAATGATTGCAGAGTTTTTGCCGTTTATGAATGAAAAAAGGTCTGCTACAACGCAAGTTAAGGCTCCACCTCAGCTACAGAAAACGGGTGCTGTTAGAGTGTCGATGGTAAGTCCTGGATTTGTATATGAACCCTATGCGCTCCGGGAGAAAATCTCGATTTGGCGGAGATGTTTTACAAGAAGTGGTTGGCGAAGAACGAAAGAGGATTTTATACGGGAGCTAAGAAGTGCCTACGCTATCGCTAAGTTAAGAAAGACTGGGTATTCGAAGAATACGTTCTACATAGAAGCACTAGAGTTATACAAACAGATTAATATTCAGATGGCCAATGGTGAGAAGAAGACGATAAGGAAAAACGTAACTGAAAGAATGTACTCTGCACTGAAGAACGAAATTAAACAAAGAGAAGCCATGTGGGATGGTGTTTACTGGGAAATGGTTGAGCCGGTTGTCAAAATCCGAACTTTACAAGCTCGACTGATTGGAATTGACCGGACGGACCTTAAGAAAGCATTTATACAACTGACACTCGAGTTTCTGACGAAGCAGAAATTTGAAGCATACGATGCAAAAGGCAATGTAGCAGCTGGAGATAAGAACAAAGAGGTGCTTGTACGTGACATATGGGTCTTTGAGAAGTCTCTTTTCCATACAGGAGCTTACTGGCGGCTCTGTGGCCGGATCAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim44

Protein Analysis

313

Amino Acids

36.49

Weight (kDa)

9.98

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim44 PF04280 142 - 292 1.1e-25 Tim44-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27395 AT5G27395
fragaria_vesca FvH4_6g05290
malus_domestica MD04G1209800.v1.1 MD12G1224400.v1.1
prunus_persica Prupe.6G330700_v2.0.a1 Prupe.6G330700_v2.0.a1
pyrus_communis pycom04g18600 pycom12g21000
rosa_chinensis RchiOBHm_Chr3g0454031
rosa_laevigata RLG00000025488
rosa_multiflora Rmu_sc0019265.1_g000003 Rmu_sc0020385.1_g000006
rosa_rugosa Rorug02G0652400
rosa_samantha Rh3AG055100 Rh3BG056700 Rh3DG057200
rosa_wichuraiana Rw3G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 875
AciI CCGC 2 cut(s) 412, 916
AclWI GGATC 2 cut(s) 61, 938
AcoI YGGCCR 2 cut(s) 573, 925
AcsI RAATTY 1 cut(s) 800
AcuI CTGAAG 2 cut(s) 195, 653
AfaI GTAC 2 cut(s) 623, 864
AflII CTTAAG 1 cut(s) 752
AgsI TTSAA 4 cut(s) 23, 43, 110, 806
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 8 cut(s) 50, 147, 179, 322, 469, 722, 836, 908
AluI AGCT 8 cut(s) 50, 147, 179, 322, 469, 722, 836, 908
Alw26I GTCTC 1 cut(s) 894
AlwI GGATC 2 cut(s) 61, 938
Ama87I CYCGRG 1 cut(s) 779
AoxI GGCC 3 cut(s) 214, 573, 925
ApeKI GCWGC 1 cut(s) 833
ApoI RAATTY 1 cut(s) 800
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
AseI ATTAAT 1 cut(s) 561
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 1 cut(s) 748
AsuC2I CCSGG 1 cut(s) 392
AsuHPI GGTGA 1 cut(s) 593
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 779
AvaII GGWCC 1 cut(s) 748
BalI TGGCCA 1 cut(s) 575
BauI CACGAG 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 596
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 845
BccI CCATC 3 cut(s) 346, 565, 662
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 363
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 894
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 752
BisI GCNGC 2 cut(s) 834, 917
BlsI GCNGC 2 cut(s) 835, 918
Bme1390I CCNGG 2 cut(s) 363, 392
Bme18I GGWCC 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 779
BmgT120I GGNCC 1 cut(s) 748
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 363, 392
BmrI ACTGGG 2 cut(s) 516, 688
BmsI GCATC 1 cut(s) 805
BmuI ACTGGG 2 cut(s) 516, 688
BpiI GAAGAC 1 cut(s) 596
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 1 cut(s) 858
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 392
BsaAI YACGTR 1 cut(s) 866
BsaWI WCCGGW 1 cut(s) 741
Bse118I RCCGGY 1 cut(s) 694
Bse1I ACTGG 3 cut(s) 511, 683, 917
Bse3DI GCAATG 1 cut(s) 832
BseBI CCWGG 1 cut(s) 363
BseGI GGATG 1 cut(s) 673
BseMI GCAATG 1 cut(s) 832
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 3 cut(s) 511, 683, 917
BseXI GCAGC 1 cut(s) 845
BsgI GTGCAG 1 cut(s) 612
BshFI GGCC 3 cut(s) 216, 575, 927
BsiHKCI CYCGRG 1 cut(s) 779
BsiSI CCGG 4 cut(s) 391, 695, 742, 928
BsmAI GTCTC 1 cut(s) 894
BsnI GGCC 3 cut(s) 216, 575, 927
BsoBI CYCGRG 1 cut(s) 779
Bsp119I TTCGAA 1 cut(s) 515
Bsp143I GATC 3 cut(s) 53, 242, 930
BspACI CCGC 2 cut(s) 412, 916
BspANI GGCC 3 cut(s) 216, 575, 927
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 2 cut(s) 61, 938
BspT104I TTCGAA 1 cut(s) 515
BspTI CTTAAG 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 832
BsrFI RCCGGY 1 cut(s) 694
BsrI ACTGG 3 cut(s) 511, 683, 917
BssAI RCCGGY 1 cut(s) 694
BssMI GATC 3 cut(s) 53, 242, 930
BssSI CACGAG 1 cut(s) 8
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 1 cut(s) 78
BstAFI CTTAAG 1 cut(s) 752
BstAPI GCANNNNNTGC 1 cut(s) 815
BstBAI YACGTR 1 cut(s) 866
BstBI TTCGAA 1 cut(s) 515
BstDEI CTNAG 3 cut(s) 318, 470, 492
BstF5I GGATG 1 cut(s) 673
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 3 cut(s) 56, 245, 933
BstMAI GTCTC 1 cut(s) 894
BstMBI GATC 3 cut(s) 53, 242, 930
BstMWI GCNNNNNNNGC 1 cut(s) 815
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 2 cut(s) 361, 390
BstSFI CTRYAG 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 845
BstV2I GAAGAC 1 cut(s) 596
BsuRI GGCC 3 cut(s) 216, 575, 927
BtsCI GGATG 1 cut(s) 673
BtsIMutI CAGTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 1 cut(s) 748
CseI GACGC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 622, 863
CviAII CATG 2 cut(s) 182, 661
CviQI GTAC 2 cut(s) 622, 863
DdeI CTNAG 3 cut(s) 318, 470, 492
DpnI GATC 3 cut(s) 55, 244, 932
DpnII GATC 3 cut(s) 53, 242, 930
DrdI GACNNNNNNGTC 1 cut(s) 875
DseDI GACNNNNNNGTC 1 cut(s) 875
EaeI YGGCCR 2 cut(s) 573, 925
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
EciI GGCGGA 1 cut(s) 427
Eco47I GGWCC 1 cut(s) 748
Eco57I CTGAAG 2 cut(s) 195, 653
Eco88I CYCGRG 1 cut(s) 779
EcoRII CCWGG 1 cut(s) 361
FaeI CATG 2 cut(s) 185, 664
FalI AAGNNNNNCTT 4 cut(s) 49, 81, 843, 875
FatI CATG 2 cut(s) 181, 660
FauNDI CATATG 1 cut(s) 872
Fnu4HI GCNGC 2 cut(s) 834, 917
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 2 cut(s) 834, 917
FspBI CTAG 1 cut(s) 542
GlaI GCGC 1 cut(s) 387
GluI GCNGC 2 cut(s) 834, 917
GsuI CTGGAG 1 cut(s) 858
HaeIII GGCC 3 cut(s) 216, 575, 927
HapII CCGG 4 cut(s) 391, 695, 742, 928
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 388
Hin1II CATG 2 cut(s) 185, 664
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HinfI GANTC 2 cut(s) 62, 106
HpaII CCGG 4 cut(s) 391, 695, 742, 928
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 4 cut(s) 128, 570, 710, 789
Hpy188III TCNNGA 4 cut(s) 10, 57, 173, 403
Hpy8I GTNNAC 1 cut(s) 676
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 5 cut(s) 168, 202, 524, 606, 865
HpyCH4V TGCA 3 cut(s) 257, 629, 818
HpyF10VI GCNNNNNNNGC 1 cut(s) 815
HpyF3I CTNAG 3 cut(s) 318, 470, 492
HpySE526I ACGT 5 cut(s) 168, 202, 524, 606, 865
Hsp92II CATG 2 cut(s) 185, 664
HspAI GCGC 1 cut(s) 386
Kzo9I GATC 3 cut(s) 53, 242, 930
LmnI GCTCC 4 cut(s) 316, 393, 466, 905
Lsp1109I GCAGC 1 cut(s) 845
LweI GCATC 1 cut(s) 805
MaeI CTAG 1 cut(s) 542
MaeII ACGT 5 cut(s) 168, 202, 524, 606, 865
MaeIII GTNAC 3 cut(s) 164, 607, 866
MalI GATC 3 cut(s) 55, 244, 932
MboI GATC 3 cut(s) 53, 242, 930
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 5 cut(s) 111, 642, 735, 800, 935
MluNI TGGCCA 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 3 cut(s) 327, 445, 847
Mox20I TGGCCA 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 524
MscI TGGCCA 1 cut(s) 575
MseI TTAA 5 cut(s) 306, 497, 561, 645, 753
Msp20I TGGCCA 1 cut(s) 575
MspA1I CMGCKG 2 cut(s) 50, 836
MspCI CTTAAG 1 cut(s) 752
MspI CCGG 4 cut(s) 391, 695, 742, 928
MspR9I CCNGG 2 cut(s) 363, 392
MvaI CCWGG 1 cut(s) 363
MwoI GCNNNNNNNGC 1 cut(s) 815
NciI CCSGG 1 cut(s) 392
NdeI CATATG 1 cut(s) 872
NdeII GATC 3 cut(s) 53, 242, 930
NlaIII CATG 2 cut(s) 185, 664
NlaIV GGNNCC 1 cut(s) 312
NmuCI GTSAC 2 cut(s) 164, 866
NspV TTCGAA 1 cut(s) 515
PaeR7I CTCGAG 1 cut(s) 779
PcsI WCGNNNNNNNCGW 1 cut(s) 521
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 2 cut(s) 62, 106
PfoI TCCNGGA 2 cut(s) 361, 390
PkrI GCNGC 2 cut(s) 835, 918
Ppu21I YACGTR 1 cut(s) 866
PshBI ATTAAT 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 748
PspXI VCTCGAGB 1 cut(s) 779
PvuII CAGCTG 2 cut(s) 50, 836
RsaI GTAC 2 cut(s) 623, 864
RsaNI GTAC 2 cut(s) 622, 863
SaqAI TTAA 5 cut(s) 306, 497, 561, 645, 753
SatI GCNGC 2 cut(s) 834, 917
Sau3AI GATC 3 cut(s) 53, 242, 930
Sau96I GGNCC 1 cut(s) 748
ScrFI CCNGG 2 cut(s) 363, 392
SfaNI GCATC 1 cut(s) 805
SfcI CTRYAG 1 cut(s) 323
Sfr274I CTCGAG 1 cut(s) 779
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 1 cut(s) 748
SlaI CTCGAG 1 cut(s) 779
SmlI CTYRAG 2 cut(s) 752, 779
SmoI CTYRAG 2 cut(s) 752, 779
Sse9I AATT 5 cut(s) 111, 642, 735, 800, 935
SsiI CCGC 2 cut(s) 412, 916
SspI AATATT 1 cut(s) 565
SspMI CTAG 1 cut(s) 542
StyD4I CCNGG 2 cut(s) 361, 390
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 5 cut(s) 171, 205, 527, 609, 868
TaqI TCGA 6 cut(s) 241, 350, 404, 515, 724, 780
TasI AATT 5 cut(s) 111, 642, 735, 800, 935
TatI WGTACW 1 cut(s) 621
TauI GCSGC 1 cut(s) 919
TfiI GAWTC 2 cut(s) 62, 106
Tru1I TTAA 5 cut(s) 306, 497, 561, 645, 753
Tru9I TTAA 5 cut(s) 306, 497, 561, 645, 753
TscAI CASTG 1 cut(s) 636
TseFI GTSAC 2 cut(s) 164, 866
TseI GCWGC 1 cut(s) 833
Tsp45I GTSAC 2 cut(s) 164, 866
TspDTI ATGAA 4 cut(s) 225, 290, 294, 390
TspGWI ACGGA 1 cut(s) 761
TspRI CASTG 1 cut(s) 636
Vha464I CTTAAG 1 cut(s) 752
VpaK11BI GGWCC 1 cut(s) 748
VspI ATTAAT 1 cut(s) 561
XapI RAATTY 1 cut(s) 800
XhoI CTCGAG 1 cut(s) 779
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.